CN114702343A - Biological additive for enhancing efficiency of alfalfa green manure - Google Patents

Biological additive for enhancing efficiency of alfalfa green manure Download PDF

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Publication number
CN114702343A
CN114702343A CN202210450780.4A CN202210450780A CN114702343A CN 114702343 A CN114702343 A CN 114702343A CN 202210450780 A CN202210450780 A CN 202210450780A CN 114702343 A CN114702343 A CN 114702343A
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Prior art keywords
alfalfa
green manure
biological additive
microbial agent
rice
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CN202210450780.4A
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Chinese (zh)
Inventor
梁秀芝
郑敏娜
韩志顺
康佳惠
陈燕妮
李霄峰
郭芳
王慧
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Institute Of Crops In High And Cold Regions Shanxi Agricultural University
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Institute Of Crops In High And Cold Regions Shanxi Agricultural University
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Priority to CN202210450780.4A priority Critical patent/CN114702343A/en
Publication of CN114702343A publication Critical patent/CN114702343A/en
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F17/00Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
    • C05F17/20Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05DINORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D9/00Other inorganic fertilisers
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G5/00Fertilisers characterised by their form
    • C05G5/20Liquid fertilisers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02WCLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00Technologies for solid waste management
    • Y02W30/40Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Inorganic Chemistry (AREA)
  • Fertilizers (AREA)

Abstract

The invention relates to the technical field of green manure synergism, in particular to a biological additive for alfalfa green manure synergism, which is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mulginosus) and Cytophaga hutchinsonii (Cytophagahutchusonii). The biological additive can effectively promote the decomposition and release of soluble phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements in alfalfa green manure and soil, and increase the absorption of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements by rice, so that the rice yield is increased, the growth of the rice can be promoted, and the disease incidence rate in the rice planting process is reduced.

Description

Biological additive for enhancing effect of alfalfa green manure
Technical Field
The invention relates to the technical field of green manure synergism, and in particular relates to a biological additive for alfalfa green manure synergism.
Background
The alfalfa is a perennial herb of the genus Medicago of the family Leguminosae, has a name of the king of pasture at home and abroad due to high yield, wide adaptability and good economic benefit, and is an excellent green manure plant resource due to the fact that the stem leaves of the alfalfa are rich in nitrogen and phosphorus.
Through the literature search of the prior art, Zhao Lu et al (2012) report that alfalfa green manure has the effects of obviously improving the soil of a rice field, promoting the growth of rice and increasing the yield in the 29 th volume 7 (page 1142-; the types and the effects of soil phosphorus-dissolving microorganisms are reported in a review article of Li Haiyun et al (2013) in No. 48 volume No. 7 (pages 1-5) of biological Notification, and the soil phosphorus-dissolving microorganisms are proposed to improve the release of solidified phosphorus in soil, but the research on measures for promoting the decomposition and the release of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum, manganese and the like in green manure and improving the yield of the green manure is not related.
Disclosure of Invention
In order to solve the problems, the invention provides a biological additive for enhancing the efficiency of alfalfa green manure.
In order to achieve the purpose, the invention adopts the technical scheme that:
the biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mularginosus) and Cytophaga hutchinsonii (Cytophagahutchuninii).
As a preferred technical scheme, the alfalfa fermentation broth is obtained by cutting alfalfa, pulping with distilled water, filtering, adding sodium chloride according to the proportion of 0.5g/Kg, sealing, and fermenting in the dark for 72 hours.
As an optimized technical scheme, the dosage of the microbial agent is 5-6% of the mass of the alfalfa fermentation liquor.
As an optimal technical scheme, the consumption of the beet molasses is 3-6% of the mass of the alfalfa fermentation liquor;
as an optimized technical scheme, the dosage of the soybean peptide is 1-3% of the mass of the alfalfa fermentation liquor.
As the preferred technical scheme, the dosage of the clear water is 6 times of the fermentation liquor of the alfalfa.
Preferably, the microbial agent contains Trichoderma longibrachiatum T61X 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m。
The invention has the following beneficial effects:
the biological additive can effectively promote the decomposition and release of soluble phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements in alfalfa green manure and soil, and increase the absorption of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements by rice, so that the rice yield is increased, the growth of the rice can be promoted, and the disease incidence rate in the rice planting process is reduced.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Preparing raw materials:
alfalfa fermentation liquor: chopping alfalfa, pulping with distilled water, filtering, adding sodium chloride at a ratio of 0.5g/Kg, sealing, and fermenting in dark for 72h to obtain the final product;
and (3) microbial agent: taking a proper amount of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mucoginosus) and cellucophagus hatsuensis (Cytophagahutchinonii) fermentation liquor for compounding, and preparing the fermentation liquor containing the trichoderma longibrachiatum T61 multiplied by 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m microbial inoculum.
Beet molasses, soybean peptide: and (5) outsourcing.
Example 1
A biological additive for enhancing the efficiency of alfalfa green manure is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of 5 percent of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 3 percent of the mass of the alfalfa fermentation liquor; the dosage of the soybean peptide is 1 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
Example 2
The biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of 6 percent of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 6 percent of the mass of the alfalfa fermentation liquor; the using amount of the soybean peptide is 3 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
Example 3
The biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent is composed of 5.5% of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 4.5 percent of the mass of the alfalfa fermentation liquor; the using amount of the soybean peptide is 2 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
The preparation method comprises the following steps:
placing the microbial agent, the fermented liquid of herba Medicaginis, beet molasses, soybean peptide and clear water in a high speed mixer, mixing for 20min, and standing for 1 hr.
Setting in the rice field:
three test groups: alfalfa green manure + example 1 group, alfalfa green manure + example 2 group, alfalfa green manure + example 3 group;
two control groups: alfalfa green manure group and blank control group;
wherein, the alfalfa green manure and the group treatment of the embodiment 1 adopt 96L of the biological additive obtained in the embodiment 1, are uniformly sprayed on the surface of fresh alfalfa grass of 1200 kg/mu, and are ploughed into the soil.
Alfalfa green manure + example 2 group treatment 100L of the biological additive obtained in example 2 was uniformly sprayed on 1200 kg/mu surface of alfalfa fresh grass and plowed into the soil.
Alfalfa green manure + example 3 group treatment 108L of the biological additive obtained in example 3 was uniformly sprayed on 1200 kg/mu surface of alfalfa fresh grass and plowed into the soil.
The alfalfa green manure treatment adopts 1200 kg/mu alfalfa fresh grass as green manure, the alfalfa fresh grass is uniformly scattered on the surface of a rice field before rice is planted, and then the alfalfa green manure is ploughed into soil to be used as a fertilizer;
the blank control treatment adopts green manure without alfalfa, biological additives and other green manure;
there were no significant differences between the groups for the remaining treatments.
As a result:
Figure 267544DEST_PATH_IMAGE002
the foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.

Claims (7)

1. The biological additive is characterized by being prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mulginosus) and Cytophaga hutchinsonii (Cytophagahutchusonini).
2. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the alfalfa fermentation broth is obtained by chopping alfalfa, pulping with distilled water, filtering, adding sodium chloride at a ratio of 0.5g/Kg, sealing, and fermenting in the dark for 72 hours.
3. The alfalfa green manure synergistic biological additive according to claim 1, wherein the amount of the microbial agent is 5-6% of the mass of the alfalfa fermentation broth.
4. The biological additive for enhancing the efficiency of the alfalfa green manure as claimed in claim 1, wherein the amount of the beet molasses is 3-6% of the mass of the alfalfa fermentation broth.
5. The biological additive for enhancing the efficiency of the alfalfa green manure as claimed in claim 1, wherein the amount of the soybean peptide is 1-3% of the mass of the alfalfa fermentation broth.
6. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the amount of the clear water is 6 times of the amount of the alfalfa fermentation broth.
7. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the microbial inoculum comprises trichoderma longibrachiatum T61 x 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m。
CN202210450780.4A 2022-04-27 2022-04-27 Biological additive for enhancing efficiency of alfalfa green manure Pending CN114702343A (en)

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Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007195542A (en) * 2005-12-27 2007-08-09 Kumiai Chem Ind Co Ltd Method for producing spore of bacterium belonging to the genus bacillus, suitable for the preparation of microorganism agrochemical
CN102887777A (en) * 2012-10-25 2013-01-23 新疆禾稼欢肥业科技有限责任公司 Polypeptide nitrogen fertilizer
CN104193549A (en) * 2014-08-11 2014-12-10 上海交通大学 Biological additive for promoting alfalfa green manure to increase crop yield
CN107325990A (en) * 2017-08-14 2017-11-07 许海霞 A kind of complex microorganism decomposing agent and its purposes for tobacco cultivation
CN108314574A (en) * 2018-01-08 2018-07-24 赵月平 It is a kind of to utilize the production method that water-soluble humic acid fertilizer is produced with the waste water of bean product
CN111705019A (en) * 2020-06-30 2020-09-25 靳凯文 Garden waste degradation microbial inoculum

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2007195542A (en) * 2005-12-27 2007-08-09 Kumiai Chem Ind Co Ltd Method for producing spore of bacterium belonging to the genus bacillus, suitable for the preparation of microorganism agrochemical
CN102887777A (en) * 2012-10-25 2013-01-23 新疆禾稼欢肥业科技有限责任公司 Polypeptide nitrogen fertilizer
CN104193549A (en) * 2014-08-11 2014-12-10 上海交通大学 Biological additive for promoting alfalfa green manure to increase crop yield
CN107325990A (en) * 2017-08-14 2017-11-07 许海霞 A kind of complex microorganism decomposing agent and its purposes for tobacco cultivation
CN108314574A (en) * 2018-01-08 2018-07-24 赵月平 It is a kind of to utilize the production method that water-soluble humic acid fertilizer is produced with the waste water of bean product
CN111705019A (en) * 2020-06-30 2020-09-25 靳凯文 Garden waste degradation microbial inoculum

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