CN114702343A - Biological additive for enhancing efficiency of alfalfa green manure - Google Patents
Biological additive for enhancing efficiency of alfalfa green manure Download PDFInfo
- Publication number
- CN114702343A CN114702343A CN202210450780.4A CN202210450780A CN114702343A CN 114702343 A CN114702343 A CN 114702343A CN 202210450780 A CN202210450780 A CN 202210450780A CN 114702343 A CN114702343 A CN 114702343A
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- CN
- China
- Prior art keywords
- alfalfa
- green manure
- biological additive
- microbial agent
- rice
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- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000219823 Medicago Species 0.000 title claims abstract description 65
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 title claims abstract description 64
- 210000003608 fece Anatomy 0.000 title claims abstract description 32
- 239000010871 livestock manure Substances 0.000 title claims abstract description 32
- 239000000654 additive Substances 0.000 title claims abstract description 22
- 230000000996 additive effect Effects 0.000 title claims abstract description 21
- 230000002708 enhancing effect Effects 0.000 title claims description 9
- 238000000855 fermentation Methods 0.000 claims abstract description 31
- 230000004151 fermentation Effects 0.000 claims abstract description 31
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 17
- 230000000813 microbial effect Effects 0.000 claims abstract description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- 235000016068 Berberis vulgaris Nutrition 0.000 claims abstract description 13
- 241000335053 Beta vulgaris Species 0.000 claims abstract description 13
- 244000068988 Glycine max Species 0.000 claims abstract description 13
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 13
- 235000013379 molasses Nutrition 0.000 claims abstract description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 13
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 7
- 241000881860 Paenibacillus mucilaginosus Species 0.000 claims abstract description 7
- 241000223262 Trichoderma longibrachiatum Species 0.000 claims abstract description 7
- 239000007633 bacillus mucilaginosus Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 241000605111 Cytophaga hutchinsonii Species 0.000 claims abstract description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 6
- 239000012153 distilled water Substances 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 3
- 238000004537 pulping Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 239000011780 sodium chloride Substances 0.000 claims description 3
- 239000002068 microbial inoculum Substances 0.000 claims description 2
- 230000002195 synergetic effect Effects 0.000 claims 1
- 241000209094 Oryza Species 0.000 abstract description 13
- 235000007164 Oryza sativa Nutrition 0.000 abstract description 13
- 235000009566 rice Nutrition 0.000 abstract description 13
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract description 10
- 239000002689 soil Substances 0.000 abstract description 10
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 7
- 239000011574 phosphorus Substances 0.000 abstract description 7
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 7
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 abstract description 5
- PWHULOQIROXLJO-UHFFFAOYSA-N Manganese Chemical compound [Mn] PWHULOQIROXLJO-UHFFFAOYSA-N 0.000 abstract description 5
- ZOKXTWBITQBERF-UHFFFAOYSA-N Molybdenum Chemical compound [Mo] ZOKXTWBITQBERF-UHFFFAOYSA-N 0.000 abstract description 5
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 5
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 abstract description 5
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 abstract description 5
- 229910052742 iron Inorganic materials 0.000 abstract description 5
- 239000011777 magnesium Substances 0.000 abstract description 5
- 229910052749 magnesium Inorganic materials 0.000 abstract description 5
- 229910052748 manganese Inorganic materials 0.000 abstract description 5
- 239000011572 manganese Substances 0.000 abstract description 5
- 229910052750 molybdenum Inorganic materials 0.000 abstract description 5
- 239000011733 molybdenum Substances 0.000 abstract description 5
- 239000011591 potassium Substances 0.000 abstract description 5
- 229910052700 potassium Inorganic materials 0.000 abstract description 5
- 239000011593 sulfur Substances 0.000 abstract description 5
- 229910052717 sulfur Inorganic materials 0.000 abstract description 5
- 239000011701 zinc Substances 0.000 abstract description 5
- 229910052725 zinc Inorganic materials 0.000 abstract description 5
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 abstract description 4
- 239000011575 calcium Substances 0.000 abstract description 4
- 229910052791 calcium Inorganic materials 0.000 abstract description 4
- 239000002609 medium Substances 0.000 abstract description 4
- 239000011573 trace mineral Substances 0.000 abstract description 4
- 235000013619 trace mineral Nutrition 0.000 abstract description 4
- 238000000354 decomposition reaction Methods 0.000 abstract description 3
- 230000001965 increasing effect Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 201000010099 disease Diseases 0.000 abstract description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 238000011282 treatment Methods 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 241000220485 Fabaceae Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000013332 literature search Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000012946 outsourcing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Tropical Medicine & Parasitology (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to the technical field of green manure synergism, in particular to a biological additive for alfalfa green manure synergism, which is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mulginosus) and Cytophaga hutchinsonii (Cytophagahutchusonii). The biological additive can effectively promote the decomposition and release of soluble phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements in alfalfa green manure and soil, and increase the absorption of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements by rice, so that the rice yield is increased, the growth of the rice can be promoted, and the disease incidence rate in the rice planting process is reduced.
Description
Technical Field
The invention relates to the technical field of green manure synergism, and in particular relates to a biological additive for alfalfa green manure synergism.
Background
The alfalfa is a perennial herb of the genus Medicago of the family Leguminosae, has a name of the king of pasture at home and abroad due to high yield, wide adaptability and good economic benefit, and is an excellent green manure plant resource due to the fact that the stem leaves of the alfalfa are rich in nitrogen and phosphorus.
Through the literature search of the prior art, Zhao Lu et al (2012) report that alfalfa green manure has the effects of obviously improving the soil of a rice field, promoting the growth of rice and increasing the yield in the 29 th volume 7 (page 1142-; the types and the effects of soil phosphorus-dissolving microorganisms are reported in a review article of Li Haiyun et al (2013) in No. 48 volume No. 7 (pages 1-5) of biological Notification, and the soil phosphorus-dissolving microorganisms are proposed to improve the release of solidified phosphorus in soil, but the research on measures for promoting the decomposition and the release of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum, manganese and the like in green manure and improving the yield of the green manure is not related.
Disclosure of Invention
In order to solve the problems, the invention provides a biological additive for enhancing the efficiency of alfalfa green manure.
In order to achieve the purpose, the invention adopts the technical scheme that:
the biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mularginosus) and Cytophaga hutchinsonii (Cytophagahutchuninii).
As a preferred technical scheme, the alfalfa fermentation broth is obtained by cutting alfalfa, pulping with distilled water, filtering, adding sodium chloride according to the proportion of 0.5g/Kg, sealing, and fermenting in the dark for 72 hours.
As an optimized technical scheme, the dosage of the microbial agent is 5-6% of the mass of the alfalfa fermentation liquor.
As an optimal technical scheme, the consumption of the beet molasses is 3-6% of the mass of the alfalfa fermentation liquor;
as an optimized technical scheme, the dosage of the soybean peptide is 1-3% of the mass of the alfalfa fermentation liquor.
As the preferred technical scheme, the dosage of the clear water is 6 times of the fermentation liquor of the alfalfa.
Preferably, the microbial agent contains Trichoderma longibrachiatum T61X 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m。
The invention has the following beneficial effects:
the biological additive can effectively promote the decomposition and release of soluble phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements in alfalfa green manure and soil, and increase the absorption of phosphorus and potassium elements, calcium, sulfur, magnesium, iron, zinc, molybdenum and manganese and other medium and trace elements by rice, so that the rice yield is increased, the growth of the rice can be promoted, and the disease incidence rate in the rice planting process is reduced.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Preparing raw materials:
alfalfa fermentation liquor: chopping alfalfa, pulping with distilled water, filtering, adding sodium chloride at a ratio of 0.5g/Kg, sealing, and fermenting in dark for 72h to obtain the final product;
and (3) microbial agent: taking a proper amount of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mucoginosus) and cellucophagus hatsuensis (Cytophagahutchinonii) fermentation liquor for compounding, and preparing the fermentation liquor containing the trichoderma longibrachiatum T61 multiplied by 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m microbial inoculum.
Beet molasses, soybean peptide: and (5) outsourcing.
Example 1
A biological additive for enhancing the efficiency of alfalfa green manure is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of 5 percent of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 3 percent of the mass of the alfalfa fermentation liquor; the dosage of the soybean peptide is 1 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
Example 2
The biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of 6 percent of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 6 percent of the mass of the alfalfa fermentation liquor; the using amount of the soybean peptide is 3 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
Example 3
The biological additive is prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent is composed of 5.5% of the mass of the alfalfa fermentation liquor; the using amount of the beet molasses is 4.5 percent of the mass of the alfalfa fermentation liquor; the using amount of the soybean peptide is 2 percent of the mass of the alfalfa fermentation liquor; the amount of the clear water is 6 times of the fermentation liquor of the alfalfa.
The preparation method comprises the following steps:
placing the microbial agent, the fermented liquid of herba Medicaginis, beet molasses, soybean peptide and clear water in a high speed mixer, mixing for 20min, and standing for 1 hr.
Setting in the rice field:
three test groups: alfalfa green manure + example 1 group, alfalfa green manure + example 2 group, alfalfa green manure + example 3 group;
two control groups: alfalfa green manure group and blank control group;
wherein, the alfalfa green manure and the group treatment of the embodiment 1 adopt 96L of the biological additive obtained in the embodiment 1, are uniformly sprayed on the surface of fresh alfalfa grass of 1200 kg/mu, and are ploughed into the soil.
Alfalfa green manure + example 2 group treatment 100L of the biological additive obtained in example 2 was uniformly sprayed on 1200 kg/mu surface of alfalfa fresh grass and plowed into the soil.
Alfalfa green manure + example 3 group treatment 108L of the biological additive obtained in example 3 was uniformly sprayed on 1200 kg/mu surface of alfalfa fresh grass and plowed into the soil.
The alfalfa green manure treatment adopts 1200 kg/mu alfalfa fresh grass as green manure, the alfalfa fresh grass is uniformly scattered on the surface of a rice field before rice is planted, and then the alfalfa green manure is ploughed into soil to be used as a fertilizer;
the blank control treatment adopts green manure without alfalfa, biological additives and other green manure;
there were no significant differences between the groups for the remaining treatments.
As a result:
the foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (7)
1. The biological additive is characterized by being prepared by mixing and fermenting a microbial agent, alfalfa fermentation liquor, beet molasses, soybean peptide and clear water, wherein the microbial agent consists of trichoderma longibrachiatum T6, Bacillus mucilaginosus (Bacillus mulginosus) and Cytophaga hutchinsonii (Cytophagahutchusonini).
2. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the alfalfa fermentation broth is obtained by chopping alfalfa, pulping with distilled water, filtering, adding sodium chloride at a ratio of 0.5g/Kg, sealing, and fermenting in the dark for 72 hours.
3. The alfalfa green manure synergistic biological additive according to claim 1, wherein the amount of the microbial agent is 5-6% of the mass of the alfalfa fermentation broth.
4. The biological additive for enhancing the efficiency of the alfalfa green manure as claimed in claim 1, wherein the amount of the beet molasses is 3-6% of the mass of the alfalfa fermentation broth.
5. The biological additive for enhancing the efficiency of the alfalfa green manure as claimed in claim 1, wherein the amount of the soybean peptide is 1-3% of the mass of the alfalfa fermentation broth.
6. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the amount of the clear water is 6 times of the amount of the alfalfa fermentation broth.
7. The biological additive for enhancing the efficiency of alfalfa green manure as claimed in claim 1, wherein the microbial inoculum comprises trichoderma longibrachiatum T61 x 1010CFU/m, Bacillus mucilaginosus (Bacillus mucoarginosus) 1X 1010CFU/m, Cytophagahutchusonii (Cytophagahutchinonii) 1X 1010CFU/m。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202210450780.4A CN114702343A (en) | 2022-04-27 | 2022-04-27 | Biological additive for enhancing efficiency of alfalfa green manure |
Applications Claiming Priority (1)
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CN202210450780.4A CN114702343A (en) | 2022-04-27 | 2022-04-27 | Biological additive for enhancing efficiency of alfalfa green manure |
Publications (1)
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CN114702343A true CN114702343A (en) | 2022-07-05 |
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CN202210450780.4A Pending CN114702343A (en) | 2022-04-27 | 2022-04-27 | Biological additive for enhancing efficiency of alfalfa green manure |
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Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007195542A (en) * | 2005-12-27 | 2007-08-09 | Kumiai Chem Ind Co Ltd | Method for producing spore of bacterium belonging to the genus bacillus, suitable for the preparation of microorganism agrochemical |
CN102887777A (en) * | 2012-10-25 | 2013-01-23 | 新疆禾稼欢肥业科技有限责任公司 | Polypeptide nitrogen fertilizer |
CN104193549A (en) * | 2014-08-11 | 2014-12-10 | 上海交通大学 | Biological additive for promoting alfalfa green manure to increase crop yield |
CN107325990A (en) * | 2017-08-14 | 2017-11-07 | 许海霞 | A kind of complex microorganism decomposing agent and its purposes for tobacco cultivation |
CN108314574A (en) * | 2018-01-08 | 2018-07-24 | 赵月平 | It is a kind of to utilize the production method that water-soluble humic acid fertilizer is produced with the waste water of bean product |
CN111705019A (en) * | 2020-06-30 | 2020-09-25 | 靳凯文 | Garden waste degradation microbial inoculum |
-
2022
- 2022-04-27 CN CN202210450780.4A patent/CN114702343A/en active Pending
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2007195542A (en) * | 2005-12-27 | 2007-08-09 | Kumiai Chem Ind Co Ltd | Method for producing spore of bacterium belonging to the genus bacillus, suitable for the preparation of microorganism agrochemical |
CN102887777A (en) * | 2012-10-25 | 2013-01-23 | 新疆禾稼欢肥业科技有限责任公司 | Polypeptide nitrogen fertilizer |
CN104193549A (en) * | 2014-08-11 | 2014-12-10 | 上海交通大学 | Biological additive for promoting alfalfa green manure to increase crop yield |
CN107325990A (en) * | 2017-08-14 | 2017-11-07 | 许海霞 | A kind of complex microorganism decomposing agent and its purposes for tobacco cultivation |
CN108314574A (en) * | 2018-01-08 | 2018-07-24 | 赵月平 | It is a kind of to utilize the production method that water-soluble humic acid fertilizer is produced with the waste water of bean product |
CN111705019A (en) * | 2020-06-30 | 2020-09-25 | 靳凯文 | Garden waste degradation microbial inoculum |
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