CN114681362B

CN114681362B - Shampoo capable of improving scalp micro-ecology and achieving high conditioning performance

Info

Publication number: CN114681362B
Application number: CN202210365981.4A
Authority: CN
Inventors: 华沈智仁; 李东翠; 吉冈正人; 成晓松; 叶茂权; 解鹏辉; 周龙兵
Original assignee: Zhanghua Cosmetics Technology Co ltd
Current assignee: Zhanghua Cosmetics Technology Co ltd
Priority date: 2022-04-08
Filing date: 2022-04-08
Publication date: 2023-02-03
Anticipated expiration: 2042-04-08
Also published as: CN114681362A

Abstract

The invention relates to the technical field of daily chemicals, and provides a shampoo capable of improving scalp microecology and high conditioning, which contains a microecological functional composition and a high conditioning hydrolyzed protein composition; the microecological functional composition comprises the following components: 0.1 to 3 weight percent of polyhydroxy propyl arginine and derivatives thereof; 0.25 to 5 weight percent of polyhydroxy sugar; 0.1 to 1 percent of salicylic acid and derivatives thereof; the high conditioning hydrolyzed protein composition comprises the following components: stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin 0.05-1 wt%; 0.05 to 1 weight percent of hydrolyzed collagen PG-propylmethylsilanediol; 0.05 to 1wt% of hydrolyzed keratin. The shampoo can provide a healthy microecological environment for scalp and hair root, and uniform protein deposition and high conditioning effect for hair by the cooperation of the microecological functional composition and the hydrolyzed protein composition.

Description

Shampoo capable of improving scalp microecology and achieving high conditioning performance

Technical Field

The invention relates to the technical field of daily chemicals, and particularly relates to a shampoo capable of improving micro-ecology of scalp and having high washing, protecting and conditioning performances.

Background

CN202110275920.4 discloses polyhydroxypropyl arginine and a derivative-polyhydroxy sugar compound thereof, and a preparation method and application thereof. The molecules of the polyhydroxypropyl arginine and the derivatives thereof are active ingredients formed by fusing glycerol, arginine and the derivatives thereof; glycerin is a high-efficiency moisturizing component; arginine and derivatives are natural moisturizing factor components, positive charges carried by the arginine and the derivatives generate strong electrostatic adsorption force with skin or damaged hair, and hydroxyl oxygen of glycerol and nitrogen groups of the arginine and the derivatives are combined to easily form hydrogen bonds with other hydrogen bond donors such as skin/damaged hair and the like, so that long-acting and lasting moisturizing effect is achieved. Further utilizes the special composite structure and the cationic electrostatic action of the poly-hydroxypropyl arginine and the derivatives to achieve more efficient interface adsorption performance, has good moisturizing performance and excellent skin feel, and can be applied to various daily chemical washing and skin care formula systems.

The scheme adopts the hydroxypropyl arginine and the derivative thereof-polyhydroxy sugar complex, and is mainly used for improving the water retention rate of skin and improving the moisturizing effect.

CN201811617577.1 discloses a hydrolyzed keratin hair conditioner, which is prepared from the following raw materials in percentage by weight: 2-5% of hydrolyzed keratin, 1-3% of hydrolyzed vegetable protein PG-propyl silanetriol, 1-3% of hydrolyzed collagen, 0.5-2% of collagen amino acids, 5-10% of glycerol, 1-3% of cetrimonium methylsulfate, 1-3% of cetearyl alcohol, 1-3% of panthenol, 1-2% of behenyl trimethyl ammonium methylsulfate, 330.1-0.3% of quaternary ammonium salt, 0.1-0.3% of guar hydroxypropyl trimethyl ammonium chloride, 0.1-0.3% of allantoin, 0.2-0.4% of DMDM hydantoin, 0.1-0.3% of EDTA disodium, 0.05-0.2% of essence and the balance of deionized water.

The scheme is mainly used for repairing damaged hair, and the used hydrolyzed protein comprises the following components: hydrolyzed keratin, hydrolyzed plant protein PG-propyl silantriol and hydrolyzed collagen. It can be seen that complex proteolytic proteins have been identified for hair repair. However, there is no case to describe the inside-out repairing effect of the complex proteins with precise triple molecular weight and chemical modification (small molecule cationic hydrolyzed keratin, medium molecule silanediol modified hydrolyzed collagen, and large molecule hydrolyzed keratin) on hair.

CN110934762A proposes a scalp multi-azimuth care composition containing dihydroxypropyl arginine HCL and application thereof, the proposal discloses a scalp multi-azimuth care composition containing arginine derivatives, which is characterized by comprising the following components in percentage by weight: 30-45% of polyhydroxy humectant with carbon chain length of C3-C6; 30-45% of pyrrolidone carboxylate; dihydroxypropyl arginine HCl 0.5-20%; 0.1 to 25 percent of bacteriostatic agent; 0.1-10% of anti-inflammatory agent; the sum of the weight percentages of the components is less than 100 percent, and the balance is water;

however, CN110934762A has the following characteristics to be noted: 1) The concentration of dihydroxypropyl arginine HCL used was 0.5% to 20%, which we considered not to be the optimal amount to solve the problem of the present invention. 2) The invention provides an elution formula with emphasis on a scalp-resident formula; 3) The related composition contains necessary bacteriostatic agents, and the invention focuses on developing an elution type formula which has bacteriostatic function and is based on no bacteriostatic agent.

In the process of removing dandruff and caring scalp, the improvement of the microecology of scalp and hairy roots is an important means for reducing dandruff and scalp itching, reducing alopecia, building scalp oil-water balance, improving scalp barrier function and improving hair nutrition and injury. Scalp microbiota is a wild, complex phenomenon that always seeks balance. Scalp microflora protects the scalp from harmful organisms, helps maintain scalp pH, and has an important barrier function, helps the scalp to attack, e.g., pollution, extreme temperatures, etc., below the outside, while producing nutrients and essential lipids for the scalp, keeping the scalp healthy.

At the present stage, no method for balancing scalp microecological theory and simultaneously realizing high hair conditioning performance is provided. Formula researches are carried out by most theories around the theories of mildly cleaning the scalp, reducing the nutrient content of the formula, thereby lightening the scalp burden, inhibiting and killing harmful flora and the like; few studies have been directed to maintaining scalp micro-ecological health using probiotics, prebiotics, metazoans, etc., but no complete solution has been proposed to balance scalp micro-ecological theory while providing highly organized wash care, particularly to methods of combining basic amino acid derivatives, polyhydroxy bioactive sugars, polycarboxyl acidic materials, and triple hydrolyzed proteins and derivatives.

Malassezia, propionibacterium and Staphylococcus, the main bacterium and fungus group that affect dandruff, in the dandruff patient, probiotic staphylococcus epidermidis decline, harmful bacterium Staphylococcus aureus rise, fungus restrictive Malassezia rise apparently; seborrhoic dermatitis patients usually have very remarkable abnormal proliferation of propionibacterium acnes, and researches show that compared with healthy scalp population, scalp flora microecology of dandruff and seborrhoic dermatitis patients is destroyed.

The technical problem to be solved by the scheme is as follows: the hair conditioner not only can kill harmful flora through simple inhibition of the bacteriostatic agent, but also can effectively improve the microecological balance state of the flora on the scalp, improve the oil-water balance and barrier function of the scalp, reduce alopecia, and simultaneously repair hair injury and improve the nutritional state of the hair with high conditioning property through the reasonable substance combination synergistic function.

Disclosure of Invention

One of the objectives of the present invention is to provide a shampoo with improved scalp micro-ecology and high conditioning performance, which can create a healthy micro-ecological environment for scalp and hair roots through the cooperation of a micro-ecological functional composition and a conditioning hydrolyzed protein composition, and simultaneously achieve an excellent hair restoration effect.

In order to achieve the aim, the invention provides a shampoo with functions of improving scalp microecology and high conditioning, which contains a microecological functional composition and a high conditioning protein hydrolysate composition;

the microecological functional composition comprises the following components:

0.1 to 3 weight percent of polyhydroxy propyl arginine and derivatives thereof;

0.25 to 5 weight percent of polyhydroxy sugar;

0.1 to 1 percent of salicylic acid and derivatives thereof;

the high conditioning hydrolyzed protein composition comprises the following components:

stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin 0.05-1 wt%;

0.05 to 1 weight percent of hydrolyzed collagen PG-propylmethylsilanediol;

0.05 to 1 weight percent of hydrolyzed keratin;

the molecular weight Mw of the stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin is 400-1000 g/mol;

the molecular weight Mw of the hydrolyzed collagen PG-propylmethylsilanediol is 1000-4000 g/mol;

the molecular weight Mw of the hydrolyzed keratin is 10000-30000 g/mol.

The shampoo for improving scalp microecology and high conditioning also comprises an oil regulator:

0.1 to 1 weight percent of maleic acid castor oil ester;

0.1-1 wt% of shea butter.

The shampoo for improving scalp microecology and high conditioning also comprises a surfactant system, an essential oil composition, a functional additive and a cationic conditioner,

in the shampoo for improving the micro-ecology of the scalp and high conditioning performance, the epiphyte living bodies comprise the following components:

7-20 wt% of sodium laureth sulfate;

0.3 to 15 weight percent of sodium lauroyl methyl aminopropionate;

1-5 wt% of cocamidopropyl betaine;

0-2 wt% of cocamide MEA;

0-2 wt% of lauryl glucoside APG.

In the above scalp micro-ecology improving, high-conditioning shampoo, the essential oil composition comprises the following components:

1-6 wt% of PEG-40 hydrogenated castor oil;

0 to 2 weight percent of polysorbate-20;

0.01 to 0.2 weight percent of Ouguibai fruit oil;

0.01 to 0.3 weight percent of rosemary essential oil;

0 to 1.5 weight percent of essence;

the functional additive comprises the following components:

0 to 0.1 percent of chelating agent

0 to 1 weight percent of anti-dandruff agent;

0-0.5 wt% of antioxidant;

0 to 1.5 weight percent of preservative;

0 to 2 weight percent of viscosity regulator;

0 to 1 weight percent of PH regulator;

the cationic conditioner is one or more of polyquaternium-10, polyquaternium-6, polyquaternium-7, polyquaternium-22, polyquaternium-47 and polyquaternium-67; the dosage of the polyquaternium is 0.1 to 1wt percent.

In the shampoo for improving the micro-ecology of the scalp and improving the conditioning performance, the structure of the poly-hydroxypropyl arginine and the derivative thereof is shown as a formula I:

in the formula I, X is a hydrogen atom or-CH ₂ CH(OH)CH ₂ (OH); y is hydrogen, alkali metal, ammonium, organic ammonium or-CH ₂ CH(OH)CH ₂ (OH)。

In the shampoo for improving the micro-ecology of the scalp and improving the conditioning performance, the poly-hydroxypropyl arginine is dihydroxypropyl arginine, wherein X is H, and Y is H.

In the shampoo capable of improving the micro-ecology of the scalp and realizing high conditioning performance, the polyhydroxy sugar is at least one of trehalose, beta-glucan, chitosan oligosaccharide, hyaluronic acid, tremella polysaccharide, fructo-oligosaccharide, maltooligosaccharide, hydrogenated hydrolyzed starch, ganoderma lucidum polysaccharide, lycium barbarum polysaccharide, lentinan, auricularia auricula polysaccharide, laminarin, pine pollen polysaccharide, marine bioactive polysaccharide, marine plant active polysaccharide and marine microbial polysaccharide.

In the shampoo for improving scalp microecology and high conditioning performance, the salicylic acid and the derivatives thereof are at least one of salicylic acid, caprylyl salicylic acid, salicylic acid MEA salt, salicylic acid TEA salt, potassium salicylate, water soluble salicylic acid, betaine salicylate, glycol salicylate and chitosan salicylate.

In the above-mentioned scalp micro-ecology improving and high conditioning shampoo, the polyhydroxy sugar is composed of the following components:

0.05 to 1 weight percent of fructo-oligosaccharide;

glycosyl trehalose 0.1-1 wt%;

0 to 1 weight percent of seaweed oligosaccharide.

It should be noted that: in the present invention, wt% represents the weight percentage of the total shampoo weight unless particularly emphasized.

In the formula, deionized water is added to make the total amount of the formula be 100wt%.

Advantageous effects

Compared with the prior art, the invention can build a healthy micro-ecological environment for scalp and hairy root through the synergy of the micro-ecological functional composition and the hydrolyzed protein composition, wherein: this solution differs from the patents referred to in the background art in that we combine cationic hydrolyzed keratin, silanediol modified hydrolyzed collagen, and hydrolyzed keratin, which otherwise would be considered by others to be less compatible in solubility in the traditional sense. The three components are matched with a special and accurate 3D molecular weight to achieve excellent layer-by-layer permeation, so that the hair conditioner has excellent conditioning performance of wet hair and dry hair. The actual repair of the hair from the cortex to the cuticle.

The different effect of the 3D protein deposition of the present protocol and the random addition of common proteins can be seen by protein fluorescence.

The invention combines the alkaline polyhydroxypropylarginine and the derivative thereof, the polyhydroxy bioactive sugar, the acidic substance salicylic acid and the compound synergistic composition of the derivative thereof, and the protein hydrolysate and the derivative thereof matched with triple molecular weights, and human and in vitro tests show that the composition can 1) balance the flora microecology of the scalp, increase the diversity of flora, promote the proliferation of beneficial bacteria (such as staphylococcus epidermidis) groups, not serve as additional nutrient substances to ensure that harmful flora (such as staphylococcus aureus and propionibacterium acnes) excessively proliferate, properly inhibit the proliferation of harmful fungus restrictive malassezia produced by scurf, and realize scalp maintenance by regulating and controlling reasonable flora proportion; 2) The shampoo containing the composition can improve scalp itching, dandruff, peculiar smell, scalp oil-water balance and hair silk conditioning by human body experiment questionnaire; 3) The triple molecular weight protein can effectively permeate into the hair layer by layer, and the hair can be efficiently repaired and conditioned.

Compared with the patent cited in the background of the invention, the scheme has the following advantages: 1) For CN110934762A, the dihydroxypropyl arginine in the scheme is less than 0.5 percent, and the combined concentration of the polyhydroxy sugar and the acid is far lower than the concentration of 30 to 45 percent of that of CN110934762A, so that good maintenance and bacteriostasis effects are achieved; 2) Although CN202110275920.4 discloses an arginine derivative, and indicates that the arginine derivative has the effects of deposition improvement, long-acting moisturizing effect improvement and skin feel improvement after adsorption synergistic enhancement, the single use of the derivative cannot achieve the effects of improving scalp micro-ecological environment, dandruff, scalp oil-water balance and barriers; 3) The composition related to CN110934762A has necessary bacteriostatic agent, the microecological composition of the patent does not necessarily contain bacteriostatic agent, and the patent does not aim at killing bacteria or fungi by using the bacteriostatic agent alone, but emphasizes the microecological balance effect of a flora. And CN110934762A only carries out the bacteriostatic performance evaluation of single fungus malassezia, the oil-water index of human body and the evaluation of head itching and scurf, and does not carry out complete single strain culture test on the composition and study on the change of the microecology of the complete flora of human scalp.

Drawings

FIG. 1 is a comparison of the abundance of bacterial flora, chao index, after 3 weeks for example 2 and comparative examples 4-6;

FIG. 2 is a comparison of the uniformity index of the bacterial flora after 3 weeks for the samples of example 2 and comparative examples 4-6;

FIG. 3 is a Shannon index comparison of bacterial flora diversity after 3 weeks for samples of example 2 and comparative examples 4-6;

FIG. 4 shows a hydrolyzed protein deposition fluorescence experiment.

Detailed Description

The invention will now be further described with reference to the following examples, which are not to be construed as limiting the invention in any way, and any limited number of modifications which can be made within the scope of the claims of the invention are still within the scope of the claims of the invention.

Example 1, comparative examples 1 to 3

Compositions containing the compositions of examples and comparative examples in Table 1, and the balance water were prepared, and the compositions were mixed and stirred uniformly for use.

Table 1 formula table

Examples 2 to 5

Shampoo examples 2 to 5 containing the composition according to the invention were prepared as shown in table 2:

table 2 formula table

The preparation method comprises the following steps:

1. heating phase A to 80 ℃, and stirring and dispersing until the phase A is completely uniform.

Pre-dispersing the phase B in a small amount of water, adding the uniformly mixed phase A at 80 ℃, and stirring and uniformly mixing.

3. And adding the substances in the phase D in sequence, continuously stirring uniformly, and then cooling.

4. When the temperature is reduced to 45 ℃, adding the phase C which is uniformly mixed in advance, and continuously stirring uniformly.

5. Adding the substances in the E phase and the F phase in sequence, and continuing stirring uniformly.

6. When the temperature is reduced to 25-30 ℃, the pH value of the citric acid in the F phase is adjusted to 5.5-5.8.

The supplier of stearyldimethylammonium hydroxypropyl hydrolyzed keratin is Japanese Kabushiki Kaisha; the stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin can be synthesized by Japanese Kabushiki Kaisha

Any one of Q, CAQ and SAQ type products in the series can achieve similar effects.

The supplier of the hydrolyzed collagen PG-propylmethylsilanediol is Japanese Kabushiki Kaisha; the hydrolyzed collagen PG-propylmethylsilanediol can be synthesized by Japanese Kabushiki Kaisha

SIG model products within the series.

The supplier of hydrolyzed keratin is the Japanese Kabushiki Kaisha. The hydrolyzed keratin can be made into Japanese-style synthetic meeting

Similar effects can be achieved by products of WK-GB or KR-30 types in the series.

Comparative examples 4 to 8

In general, the same as example 2, except for the formulation, reference is made in particular to table 3 below:

table 3 formula table

Performance test

1. Scalp micro-ecological test data

1.1 in vitro bacteria and fungi selective qualification experiment:

the experimental method comprises the following steps:

bacterial culture experiments: to the BPW medium containing the composition of Table 1, staphylococcus epidermidis (strain: NBRC 100911), staphylococcus aureus (strain: NBRC 13276), or Propionibacterium acnes (strain: NBRC 107605) were added individually and mixed uniformly, and the mixture was cultured at 37 ℃ for 48 hours. The optical concentration (OD 620 value) was measured at each stage (after mixing, after 24 hours of culture, and after 48 hours of culture). In addition, GAM agar plates after 48 hours of culture were observed. If the value of OD620 is higher than that of the bacterial culture hole plate without the reference group, the additive has stronger proliferation effect on the cultured strains; if there is no difference in the OD620 value compared to the reference bacterial culture well plate without the added group, the composition has no obvious proliferation or inhibition effect on the cultured strain; if the OD620 value is lower compared to the reference non-supplemented bacterial culture well plate, the composition is indicative of an inhibitory effect on the cultured species.

Fungal culture experiments: the sterilized filter paper was placed in the low nutrient LNA agar medium containing the composition of Table 1

About 5. Mu.l of Malassezia (Malassezia furfur) was sown on the filter paper and cultured at 30 ℃ for 7 days. The proliferation range of the cultured malassezia was evaluated. Taking the initial circle center of the cultured bacteria as a concentric circle, culturing the malassezia by referring to blank non-added substances after culture, and if the range of the concentric circle of the sample is enlarged, indicating that the malassezia has a proliferation effect; if the concentric circle range of the sample is similar to that of the blank reference, the sample has the function of inhibiting the proliferation of malassezia.

The OD620 values of the in vitro bacterial cultures and the size of the proliferation cycle of the fungus Malassezia are shown in Table 4:

TABLE 4 OD620 values of in vitro bacterial cultures and growth cycle size data of the fungus Malassezia

By in vitro culture of individual flora, it can be seen that the combined functional bag (dihydroxypropyl arginine HCL + polyhydroxy glycoconjugate + salicylic acid) has a balance regulating effect on bacteria and fungi on the surface of the scalp, i.e. can selectively promote proliferation, do not act or inhibit proliferation according to the species of bacteria and fungi, thereby balancing the flora changes on the scalp: 1) significant proliferation-promoting effects on beneficial groups such as staphylococcus epidermidis in table 4, 2) main harmful groups such as staphylococcus aureus and propionibacterium acnes affecting dandruff, not over-proliferating as additional nutrients (i.e., not proliferating harmful bacteria as additional nutrients, conventionally many sugars and ester additives have proliferation-promoting effects on harmful bacteria to burden the scalp) and 3) moderate inhibition of proliferation of harmful fungi-limiting malassezia produced by dandruff. Through the effects, the reasonable flora balance proportion is regulated and controlled, and the maintenance of scalp microecological balance can be realized.

Comparative example 1, which lacks dihydroxypropylarginine HCl and contains only complex polyhydroxy sugar and salicylic acid composition as compared to example 1, could control the hyperproliferation of the harmful bacteria staphylococcus aureus and propionibacterium acnes to some extent, but could not effectively promote the proliferation of the beneficial bacteria staphylococcus epidermidis, and at the same time, could not effectively inhibit the proliferation of the limiting malassezia, and thus could not regulate the flora balance state.

Compared with the example 1, the composition which lacks complex polyhydroxy sugar and only contains dihydroxypropyl arginine HCl and salicylic acid can control the excessive proliferation of harmful bacteria staphylococcus aureus and propionibacterium acnes to a certain extent, effectively inhibit the proliferation of the restrictive malassezia but cannot effectively promote the proliferation of beneficial bacteria staphylococcus epidermidis, so that the flora balance state cannot be adjusted.

Comparative example 3, which lacks salicylic acid and contains only dihydroxypropylarginine HCl and the polyhydroxy saccharide composition as compared to example 1, failed to control the excessive proliferation of harmful bacteria staphylococcus aureus and propionibacterium acnes, failed to effectively inhibit the proliferation of the limited malassezia bacteria, and failed to effectively promote the proliferation of the beneficial bacteria staphylococcus epidermidis, and failed to regulate the state of flora balance.

The salicylic acid plays a very important role in the processes of cutin exfoliation, scalp oil-water balance, bacteriostasis and flora balance during excessive keratinization, and the main reason is that carboxyl of the salicylic acid and dihydroxypropyl arginine can form a strong hydrogen bond structure, the structure is based on the strong persistence formed by the dihydroxypropyl arginine and polyhydroxy saccharides, and meanwhile, the hydrogen bond structure is also beneficial to the exertion of the flora balance and the regulation and control of the oil-water balance.

The above examples and comparative examples, cultured in vitro from single bacterial and fungal strains, illustrate that the compositions of the present invention are lacking in order to more effectively regulate the micro-ecological balance of the scalp flora.

2. Detecting genes of micro-ecological bacteria flora on human scalp: balance the micro-ecology of flora on the scalp, and increase the richness, uniformity and diversity of flora

The results of in vitro culture of a single strain are set forth in 1.1, which indicates the basic direction for the composition to proliferate beneficial and harmful bacteria. Further to better illustrate the effect of the compositions described herein on the homeostasis of the human scalp micro-ecological flora, we performed human experiments on the changes in the scalp micro-ecological bacterial flora.

To rule out the effects of gender, the study was conducted on only 12 male volunteers with severe seborrheic dermatitis and dandruff problems. The volunteers were divided into three groups of four persons, and sampling and comparative analysis of bacterial diversity and composition characteristics of the left and right head surfaces of the volunteers were conducted before and after using the shampoos of example 2 (left head) and comparative example 4 or comparative example 5 or comparative example 6 (right head) for 3 consecutive weeks on the left and right heads, respectively. Sample collection follows the conventional 16sRNA sampling detection method, namely, a clean cotton swab is used for wiping the corresponding part of the scalp, and 16S rRNA gene sequence data obtained by high-throughput pyrosequencing is used for analyzing and evaluating bacterial communities.

The alpha diversity analysis reflects the diversity of species in the ecosystem, and comprises comprehensive indexes of richness, uniformity and diversity. 1) And (3) richness: the more abundant the species class, the higher the diversity. Richness is characterized by the Chao index: chao1 is an index for estimating the number of OTUs contained in the community by using a Chao1 algorithm, and the larger the Chao is, the larger the number of OTUs contained in the community is, the larger the abundance of the community is. 2) Uniformity: the number of different species is uniform, the more uniform the diversity. 3) Shannon index: one of the indexes of diversity used to estimate microorganisms in a sample is the commonly used index of alpha diversity. The larger the Shannon value, the higher the colony diversity (including abundance and uniformity).

The richness of the bacterial flora, chao index, was compared among 12 volunteers after three weeks of use of the shampoos containing example 2 and comparative examples 4-6, and the data are shown in fig. 1: compared with the initial value of the abundance of the bacterial flora in the scalp micro-ecology of 12 volunteers before any test sample is used, the shampoo in the embodiment 2 can obviously improve the abundance Chao index of the bacterial flora, and the abundance Chao indexes of the bacterial flora are reduced in different degrees in the comparative examples 4, 5 and 6.

The uniformity of bacterial flora was compared in 12 volunteers after three weeks of use of the shampoos containing example 2 and comparative examples 4-6, and the data is shown in fig. 2: the shampoo of the embodiment 2 can obviously improve the uniformity of the bacterial flora, the comparative examples 4 and 6 reduce the uniformity of the bacterial flora to different degrees, and the comparative example 5 has no obvious change.

The diversity of bacterial flora Shannon index of 12 volunteers after three weeks of use of the shampoo containing example 2 and comparative examples 4-6 was compared, and the data is shown in figure 3: the shampoo of the embodiment 2 can obviously improve the diversity Shannon index of the bacterial flora, and the comparative examples 4, 5 and 6 reduce the diversity Shannon index of the bacterial flora to different degrees.

In conclusion, the gene analysis of the bacterial flora of the human scalp microecology shows that the embodiment 2 of the invention can improve the abundance, uniformity and diversity of the scalp flora, thereby creating more beneficial conditions for the microecology balance of the scalp flora, and the comparative examples 4, 5 and 6 can not have positive influence on the abundance, uniformity and diversity of the flora.

Subjective evaluation of volunteers and in vitro experiment of protein deposition: high regulation, uniform and effective protein deposition, improvement of oil-water balance of scalp and pruritus

Subjective evaluation of volunteers: aiming at scalp pruritus, excessive sebum secretion or abnormal dry and dandruff of scalp, 24 male and female experimental objects of 20-60 years old are selected, the initial scalp and hair state is observed, the experimental objects are used for 1 time every 1 day, a double-blind shampoo sample (containing example 2 and comparative examples 4, 5, 6, 7 and 8) is continuously used for 4 weeks, the performance of the shampoo is scored according to the self state by subjective feedback, and the evaluation performance index is mainly as follows: the grading is divided into 5 grades: very severe (score 1), slightly severe (score 2), medium (score 3), significantly improved (score 4), and completely improved (score 5). The results are shown in Table 5 after the statistical scoring and averaging.

In vitro protein deposition experiments: the conditioning and repair properties of hair are related to the amount and uniformity of deposition of the hydrolyzed protein, conditioning agents, moisturizing agents, and ester-forming agents on the hair. In order to compare the hair conditioning conditions of the examples and the comparative examples, the protein deposition amount and uniformity of the hair after being washed and protected by different shampoos were tested by using a fluorescence microscope photographing experiment. The higher the fluorescence intensity, the more uniform the fluorescence distribution, indicating the better the coverage of the protein on the hair, the higher the corresponding hair restoration and opsonization.

The specific experimental operation is as follows:

1) And (3) perming pretreatment: standard hair tresses (hair taken from asian hair, 2.3 g, 15 cm) were subjected to a standard wound treatment using a solution containing 7% sodium bromate and 0.5% disodium hydrogen phosphate (ph 6.5), and the above permanent wave treatment 3 was repeated for use;

2) Protein deposition by fluorescence microscopy:

preparing fluorescent protein shampoo: preparing corresponding protein connected with fluorescent group into corresponding shampoo samples according to the proportion and the concentration of the corresponding protein connected with the fluorescent group in example 2 and comparative examples 4-8

Hair fluorescent dyeing: the hair treated by 1) permanent waving was put into a fluorescent shampoo containing 10 times of dilution, heated to 40 ℃ and left to stand (40 ℃,10 minutes).

Wiped with a Kimwipe and rinsed with ion-exchanged water (20 seconds).

The washed (ion-exchanged water in 3) samples were subjected to microscopic observation.

And (3) observation conditions: 400 multiplying power, a U-MNIBA fluorescence filter, emission wavelength: absorption wavelength of 470 to 495 nm: 510-550nm

TABLE 5 volunteer test results

The results of volunteers' evaluation of shampoo properties are averaged and combined as shown in table 5. The comparison results show that the shampoo containing the microecological composition (dihydroxypropyl arginine and derivatives thereof, polyhydroxy sugar composition, salicylic acid and derivatives) and the triple-compounded protein composition (small molecular weight (Mw = 400-1000 g/mol) stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin, medium molecular weight (Mw = 1000-4000 g/mol) hydrolyzed collagen PG-propyl methyl silane glycol, and large molecular weight (Mw =10,000-30,000g/mol) hydrolyzed keratin) can bring oil-water balance of scalp, improve the problems of over-dry scalp or oil odor, improve pruritus of scalp, and simultaneously, can take care of high dry and wet conditioning of hair. While comparative examples 4, 5, 6 were not effective in improving the condition of the volunteers' scalp due to the absence of any of the micro-ecological compositions, similar to the results of scalp micro-ecology described above. Comparative examples 7, 8 lack one of the triple molecular weight protein compositions of the present invention and are unable to balance high conditioning of hair.

To further illustrate this situation, fig. 4 is a comparison graph of the results of the fluorescence experiment of protein deposition, and the results are qualitatively counted as two indicators of the amount of protein deposition (intensity of fluorescence) and the uniformity of protein deposition in table 5. The results show that shampoo containing the microecological composition and a triple-compounded protein composition (small molecular weight (Mw = 400-1000 g/mol) stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin, medium molecular weight (Mw = 1000-4000 g/mol) hydrolyzed collagen PG-propyl methyl silane glycol, and large molecular weight (Mw =10,000-30,000g/mol) hydrolyzed keratin) can bring uniform high deposition amount of protein, thereby having better hair silk conditioning and repairing effects. Comparative examples 4, 5, 6, 7, and 8, however, lack any of the probiotic compositions, or any of the triple molecular weight protein compositions of the present invention, did not balance the balance of hair conditioning with scalp conditioning.

Claims

1. A shampoo with high conditioning performance for improving scalp microecology is characterized by comprising a microecological functional composition and a high conditioning hydrolyzed protein composition;

the microecological functional composition comprises the following components:

0.1 to 3wt% of polyhydroxypropylarginine and derivatives thereof;

0.25 to 5wt% of polyhydroxy sugar;

salicylic acid and derivatives thereof 0.1 to 1%;

stearyl dimethyl ammonium hydroxypropyl hydrolyzed keratin 0.05 to 1wt%;

0.05 to 1wt% of hydrolyzed collagen PG-propylmethylsilanediol;

0.05 to 1wt% of hydrolyzed keratin;

the molecular weight Mw of the stearyldimethylammonium hydroxypropyl hydrolyzed keratin is 400 to 1000g/mol;

the molecular weight Mw of the hydrolyzed collagen PG-propylmethylsilanediol is 1000 to 4000g/mol;

the molecular weight Mw of the hydrolyzed keratin is 10000 to 30000g/mol;

the structure of the poly-hydroxypropyl arginine and the derivative thereof is shown as a formula I:

formula I

In the formula I, X is a hydrogen atom or-CH ₂ CH(OH)CH ₂ (OH); y is hydrogen, alkali metal, ammonium, organic amine or-CH ₂ CH(OH)CH ₂ (OH)；

The salicylic acid and its derivatives are at least one of salicylic acid, octanoyl salicylic acid, salicylic acid MEA salt, salicylic acid TEA salt, potassium salicylate, water soluble salicylic acid, betaine salicylate, glycol salicylate, and chitosan salicylate.

2. The scalp micro-ecology improving high-conditioning shampoo according to claim 1, further comprising a fat conditioner:

0.1 to 1wt% of maleic acid castor oil ester;

0.1 to 1wt% of shea butter.

3. A scalp micro-ecology improving, high-conditioning shampoo according to claim 1 further comprising an surfactant system, an essential oil composition, a functional additive, a cationic conditioner;

the functional additive comprises the following components by taking the total weight of the shampoo capable of improving the scalp microecology and realizing high conditioning as 100 percent:

0 to 0.1% of a chelating agent

0 to 1wt% of an antidandruff agent;

0 to 0.5wt% of an antioxidant;

0 to 1.5wt% of preservative;

0 to 2wt% of viscosity regulator;

0 to 1wt% of a pH regulator.

4. The scalp micro-ecology improving and high-conditioning shampoo according to claim 3, wherein the surfactant has the following components:

7-20wt% of sodium laureth sulfate;

0.3 to 15wt% of sodium lauroyl methylaminopropionate;

1 to 5wt% of cocamidopropyl betaine;

0-2wt% of cocamide MEA;

0 to 2wt% of lauryl glucoside APG.

5. The scalp micro-ecology improving high-conditioning shampoo according to claim 3, characterized in that the essential oil composition comprises the following components:

1-6 wt% of PEG-40 hydrogenated castor oil;

polysorbate-20 to 2wt%;

0.01 to 0.2wt% of the fruit oil of the Ougelia;

rosemary essential oil 0.01 to 0.3wt%;

0 to 1.5wt% of essence;

the cationic conditioner is polyquaternium, and the polyquaternium is one or more of polyquaternium-10, polyquaternium-6, polyquaternium-7, polyquaternium-22, polyquaternium-47 and polyquaternium-67; the dosage of the polyquaternium is 0.1 to 1wt%.

6. The scalp micro-ecology improving and high-conditioning shampoo according to claim 5, wherein the polyhydroxypropylarginine is dihydroxypropylarginine, wherein X is H and Y is H.

7. The shampoo for improving the microecology of the scalp and improving the conditioning performance as claimed in any one of claims 1 to 4, wherein the polyhydroxy sugar is at least one of trehalose, beta-glucan, chitosan oligosaccharide, hyaluronic acid, tremella polysaccharide, fructo-oligosaccharide, maltooligosaccharide, hydrogenated hydrolyzed starch, ganoderma lucidum polysaccharide, lycium barbarum polysaccharide, lentinan, auricularia auricular polysaccharide, laminarin, pine pollen polysaccharide, marine plant active polysaccharide and marine microbial polysaccharide.

8. The scalp micro-ecology improving high-conditioning shampoo according to claim 6, wherein the polyhydroxy sugar consists of:

0.05 to 1wt% of fructo-oligosaccharide;

glycosyl trehalose 0.1 to 1wt%;

and 0-1wt% of algal oligosaccharide.