CN114642616B - Mulberry leaf polyphenol-based mouthwash and preparation method thereof - Google Patents
Mulberry leaf polyphenol-based mouthwash and preparation method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/345—Alcohols containing more than one hydroxy group
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/02—Stomatological preparations, e.g. drugs for caries, aphtae, periodontitis
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- A61P31/02—Local antiseptics
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q11/00—Preparations for care of the teeth, of the oral cavity or of dentures; Dentifrices, e.g. toothpastes; Mouth rinses
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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Abstract
The invention discloses mulberry leaf polyphenol-based mouthwash and a preparation method thereof, wherein the mulberry leaf polyphenol-based mouthwash comprises the following components: the mulberry leaf polyphenol extract, black tea, xylitol, honeysuckle, wild chrysanthemum flower and amur corktree bark, and the balance of water; the preparation method comprises the following steps: soaking black tea, honeysuckle, wild chrysanthemum flower and phellodendron bark in a certain proportion by pure water, boiling, and decocting with small fire to obtain a first decocted soup; taking out the first decocted medicinal soup, adding pure water into the pot, and decocting again to obtain the second decocted medicinal soup; mixing the decoction obtained by decocting twice, adding folium Mori polyphenol extract and xylitol, adding water to 100%, mixing, and pasteurizing. By improving and innovating the components of the mouthwash, the invention not only enhances the antibacterial effect on the basis of cleaning the oral cavity, but also enhances the efficacy of preventing decayed teeth and canker sores, reduces the oral problems of contemporary people, and has better life.
Description
Technical Field
The invention relates to the technical field of mouthwashes, in particular to a mulberry She Duofen-based mouthwash and a preparation method thereof.
Background
The mouthwashes in the market are various, and most mouthwashes can achieve the effects of cleaning the oral cavity and keeping fresh, but have the defects in preventing dental caries and dental ulcer. With the increasing pressure of modern people, the diet is irregular, and the two diseases are more common. At present, people pay more attention to the oral health problem, and meanwhile, the pursuit of beauty promotes the vigorous development of the mouthwash related industry, and focuses more on the technical improvement on dental caries and dental ulcer.
Caries is commonly known as dental caries and dental caries, and is a bacterial disease, and the main bacteria causing caries are streptococcus mutans, streptococcus light and streptococcus blood, which can secondary pulpitis and periapical periodontitis, and even can cause alveolar bone and jawbone inflammation.
Canker sores are commonly known as "aphtha", and are a common ulcerative injury disease occurring on oral mucosa. The occurrence of canker sores is the result of a combination of factors including localized trauma, stress, food, medications, malnutrition, altered hormone levels, and vitamin or trace element deficiency; bacteria such as Streptococcus sanguis and helicobacter pylori are also closely related to canker sores.
Sang Sheshi dried leaves of Morus alba L.China is the country of the world where the mulberry planting area is the largest and the annual output of mulberry leaves is happy. The mulberry leaf is rich in various active ingredients such as flavonoid, alkaloids, polysaccharides and the like, has various functions of resisting oxidation, reducing blood fat, reducing blood sugar and the like, and can be widely applied. Related researches show that mulberry leaf polyphenol, namely a bioactive phenol secondary metabolite extracted from mulberry leaves, is an important material basis for forming the oxidation capacity of Sang Shekang. The mulberry leaf polyphenol has stronger DPPH free radical, hydroxyl free radical and superoxide anion free radical scavenging capability. Although the mulberry leaves and polyphenol extracts thereof in Chongqing are rich in resources, the mulberry leaves are not high in utilization rate, are mostly used for raising silkworms, a large amount of mulberry leaves are not effectively utilized, the resource waste degree is high, and especially the mulberry leaves polyphenol is not effectively applied as an active ingredient of the mouthwash.
The invention enhances the effective application of the mulberry leaf polyphenol natural product, improves and innovates the components of the mouthwash, not only enhances the antibacterial effect on the basis of cleaning the oral cavity, but also enhances the efficacy of preventing decayed teeth and canker sores, reduces the oral problems of contemporary people, and has better life.
Disclosure of Invention
The invention aims to provide a mulberry She Duofen-based mouthwash and a preparation method thereof, which can enhance the antibacterial effect of the mouthwash and prevent dental caries and dental ulcer.
In order to achieve the above purpose, the present invention provides the following technical solutions:
1. a mulberry leaf polyphenol-based mouthwash comprises the following components: the mulberry leaf polyphenol extract, black tea, xylitol, honeysuckle, wild chrysanthemum flower, phellodendron bark and the balance of water.
Preferably, the mulberry leaf polyphenol extracting solution comprises the following raw materials in percentage by weight: black tea: xylitol: honeysuckle flower: wild chrysanthemum flower: phellodendron = 5-8: 3 to 6:2 to 6:1.2 to 1.6:1 to 2.5: 2-3, and the balance being water.
Preferably, the raw materials comprise the following components in terms of total volume of 50 mL: 3.22mL of mulberry leaf polyphenol extract, 2.53g of black tea, 2g of xylitol, 0.75g of honeysuckle, 1.25g of wild chrysanthemum flower, 1.25g of amur corktree bark and the balance of water.
2. A preparation method of a mulberry leaf polyphenol-based mouthwash comprises the following steps:
soaking black tea, honeysuckle, wild chrysanthemum flower and phellodendron bark in a certain proportion by pure water, boiling, and decocting with small fire to obtain a first decocted soup;
taking out the first decocted medicinal soup, adding pure water into the pot, and decocting again to obtain the second decocted medicinal soup;
mixing the decoction obtained by decocting twice, adding folium Mori polyphenol extract and xylitol, adding water to 100%, mixing, and pasteurizing.
Preferably, the volume of water added during soaking of each Chinese medicinal component is 1-3 cm above the medicinal surface.
Preferably, the volume of water added during the second decoction is the same as the volume during the first soaking.
Preferably, the time for soaking, decocting and re-decocting the traditional Chinese medicine components is 30min.
Wherein, the pharmacological actions of the components are as follows:
mulberry leaf polyphenol extracting solution: antibacterial and biofilm elimination;
black tea: preventing dental caries and resisting oxidation;
xylitol: a sweetener to improve the mouth feel of the mouthwash;
wild chrysanthemum flower: clearing heat and detoxicating, dispelling wind and dissipating heat;
honeysuckle flower: clearing heat and detoxicating, anti-inflammatory, tonifying deficiency and treating wind;
cortex Phellodendri: clearing heat and drying dampness, purging fire and removing steam, and detoxicating and treating sore.
The beneficial effects are that:
the invention is based on the blending of mulberry leaf polyphenol and the preparation of the mouthwash, thereby enhancing the effective application of mulberry leaf polyphenol natural products. Through improvement and innovation of the components of the mouthwash, on the basis of cleaning the oral cavity, the antibacterial effect is enhanced, meanwhile, the effects of preventing decayed teeth and canker sores are enhanced, the oral cavity problem of contemporary people is relieved, and better life is provided.
Drawings
FIG. 1 is a response surface diagram of interaction of mulberry leaf polyphenol and dark tea affecting comprehensive indexes of mouthwash;
FIG. 2 is a response surface graph of interaction of mulberry leaf polyphenols with wild chrysanthemum for influencing comprehensive indexes of mouthwash;
FIG. 3 is a response surface graph of interaction of black tea and wild chrysanthemum affecting comprehensive indexes of mouthwash;
FIG. 4 is a graph showing the effect of the polyphenol extract from mulberry leaves on the antibacterial effect of mouthwash in the single factor test results in example 2;
FIG. 5 is a graph showing the effect of dark tea content on the antibacterial effect of mouthwash in the single factor test results of example 2;
FIG. 6 is a graph showing the effect of the amounts of honeysuckle, wild chrysanthemum and phellodendron on the mouthwash content in the single factor test result of example 2.
Detailed Description
The invention is further described in detail below in connection with specific embodiments.
Example 1
In order to further prove that the mouthwash has a strong antibacterial effect, the embodiment combines experimental researches to prove that the mulberry leaf polyphenol mouthwash has good efficacy on oral health.
1. Bacteriostasis experiment
A single colony of Staphylococcus aureus was cultured in 20mL of LB at 37℃with shaking for 16h. Mixing 1mL of bacterial solution with 9mL of mulberry leaf polyphenol mouthwash, performing gradient dilution with 0.85% sterile physiological saline, and respectively diluting to 10 -1 、10 -2 、10 -3 、10 -4 、10 -5 And 10 -6 . Under sterile conditions, the sample dilutions were dripped onto nutrient agar plates for colony counting and incubated at 37℃for 16h.
2. Experiment for eliminating biofilm
Taking a single bacterium of staphylococcus aureusFalls into 20mL of LB, and is cultured for 16h at 37 ℃. 1mL of the bacterial liquid was mixed with 9mL of mulberry leaf polyphenol mouthwash, and added to a 50mL sterile centrifuge tube containing stainless steel (2.0X12.0X10.1 cm). After stationary culture at 32℃for 48 hours, the bacterial culture was removed and the stainless steel sheet was washed 2 times with 10mL of 0.85% sodium chloride. The liquid in the centrifuge tube was removed and the stainless steel plate was washed 2 times with 0.85% sodium chloride. After all the washing buffer was removed, 10mL of 0.2% crystal violet was added to the centrifuge tube and treated at 25℃for 30min, and the stainless steel plate was washed with 0.85% sodium chloride 2 times and naturally air-dried. Subsequently, the biofilm on the stainless steel plate was dissolved with 1mL of 95% ethanol, and OD was measured 600 The smaller the value, the smaller the amount of biofilm, the better the biofilm removal effect of the mouthwash.
Example 2
Single factor test: determining the influence of the content of each component on the antibacterial effect by controlling the variable, soaking the black tea, the honeysuckle, the wild chrysanthemum and the phellodendron in proportion with pure water, boiling, and decocting with small fire to obtain a medicinal soup; adding pure water into the pot, and decocting again to obtain decoction. Mixing the two obtained medicinal decoctions, adding folium Mori polyphenol extract and xylitol, mixing with water to 100%, and pasteurizing under the variable setting conditions shown in table 1.
TABLE 1 mouthwash Each component content gradient
The results are shown in figures 4-6, and the contents of the honeysuckle and the phellodendron have remarkable differences and have the content with the optimal antibacterial effect, so that the content is directly determined. The optimal content ratio is determined by performing response surface test because the polyphenol extract of mulberry leaves, the black tea and the wild chrysanthemum have no significant difference.
Example 3
A preparation method of a mulberry leaf polyphenol-based mouthwash comprises the following raw materials in percentage by weight, 2.5mL of mulberry leaf polyphenol extract, 3g of black tea, 2g of xylitol, 0.75g of honeysuckle, 0.88g of wild chrysanthemum, 1.25g of phellodendron and the balance of water. Soaking the Chinese medicinal materials in pure water for 30min, boiling, and decocting with slow fire for 30min to obtain decoction; adding pure water into the pot, and decocting for 30min again to obtain decoction. Mixing the two obtained medicinal soup, adding folium Mori polyphenol extract and xylitol, mixing with water to 100%, and pasteurizing.
Example 4
A preparation method of a mulberry leaf polyphenol-based mouthwash comprises the following raw materials in percentage by weight, 2.5mL of mulberry leaf polyphenol extract, 2.25g of dark green tea, 2g of xylitol, 0.75g of honeysuckle, 0.5g of wild chrysanthemum, 1.25g of phellodendron and the balance of water. Soaking the Chinese medicinal materials in pure water for 30min, boiling, and decocting with slow fire for 30min to obtain decoction; adding pure water into the pot, and decocting for 30min again to obtain decoction. Mixing the two obtained medicinal soup, adding folium Mori polyphenol extract and xylitol, mixing with water to 100%, and pasteurizing.
Example 5
A preparation method of a mulberry leaf polyphenol-based mouthwash comprises the following raw materials in percentage by weight, 2.5mL of mulberry leaf polyphenol extract, 2.25g of dark green tea, 2g of xylitol, 0.75g of honeysuckle, 1.25g of wild chrysanthemum, 1.25g of golden cypress and the balance of water. Soaking the Chinese medicinal materials in pure water for 30min, boiling, and decocting with slow fire for 30min to obtain decoction; adding pure water into the pot, and decocting for 30min again to obtain decoction. Mixing the two obtained medicinal soup, adding folium Mori polyphenol extract and xylitol, mixing with water to 100%, and pasteurizing.
The remaining examples are similar to examples 3-5, except for the amounts of polyphenol extract from mulberry leaves, black tea, honeysuckle, wild chrysanthemum and phellodendron, which are shown in Table 2.
TABLE 2 summary of the effects of mouthwash inhibition and biofilm removal for each example formulation
Note that: total index = bacteriostatic effect x 0.5+ biofilm x 0.5.
Response surface analysis and optimal condition determination: the response surface analysis chart (figures 1-3) is drawn by using Design-Expert 8.0, and as can be seen from the response surface chart, interaction exists among the mulberry leaf polyphenol extract (A), the black tea content (B) and the wild chrysanthemum content (C), and the interaction between the black tea content (B) and the wild chrysanthemum content (C) is not obvious. And combining a response surface and a regression model of the influence of interaction of all factors on the comprehensive index of the mouthwash, and when the content (A) of the polyphenol extract of the mulberry leaves, the content (B) of the black tea and the content (C) of the wild chrysanthemum are respectively 3.22mL,2.53g and 1.25g, the effect of the comprehensive index is predicted to be optimal.
The above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered by the scope of the claims of the present invention.
Claims (6)
1. A mulberry leaf polyphenol-based mouthwash is characterized in that: the composition comprises the following components: the mulberry leaf polyphenol mouthwash comprises a mulberry leaf polyphenol extracting solution, black tea, xylitol, honeysuckle, wild chrysanthemum flower and phellodendron bark, and the balance of water, wherein the raw materials of the polyphenol mouthwash comprise the following components in percentage by weight: black tea: xylitol: honeysuckle flower: wild chrysanthemum flower: phellodendron = 5-8: 3 to 6:2 to 6:1.2 to 1.6:1 to 2.5:2 to 3.
2. The mulberry leaf polyphenol-based mouthwash of claim 1, wherein: the raw materials are calculated according to the total volume of the mouthwash of 50mL, and comprise the following components: 3.22mL of mulberry leaf polyphenol extract, 2.53g of black tea, 2g of xylitol, 0.75g of honeysuckle, 1.25g of wild chrysanthemum flower, 1.25g of amur corktree bark and the balance of water.
3. A method for preparing the mulberry leaf polyphenol based mouthwash according to claim 1 or 2, wherein: the method comprises the following steps:
soaking black tea, honeysuckle, wild chrysanthemum flower and phellodendron bark in a certain proportion by pure water, boiling, and decocting with small fire to obtain a first decocted soup;
taking out the first decocted medicinal soup, adding pure water into the pot, and decocting again to obtain the second decocted medicinal soup;
mixing the decoction obtained by decocting twice, adding folium Mori polyphenol extract and xylitol, adding water to 100%, mixing, and pasteurizing.
4. A method for preparing a mulberry leaf polyphenol based mouthwash according to claim 3, wherein: the volume of water added during soaking of each Chinese medicinal component is 1-3 cm above the medicinal surface.
5. The method for preparing the mulberry leaf polyphenol-based mouthwash according to claim 4, which is characterized in that: when the traditional Chinese medicine components are decocted for the second time, the volume of added water is the same as the volume of the primary soaking.
6. The method for preparing the mulberry leaf polyphenol-based mouthwash according to claim 5, wherein the method comprises the following steps: the soaking, decocting and re-decocting time of each traditional Chinese medicine component is 30min.
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| CN103735473A (en) * | 2013-12-04 | 2014-04-23 | 何小刚 | Herbal mouthwash and preparation method thereof |
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| CN103735473A (en) * | 2013-12-04 | 2014-04-23 | 何小刚 | Herbal mouthwash and preparation method thereof |
| CN103735437A (en) * | 2013-12-04 | 2014-04-23 | 何小刚 | Herbal mouthwash |
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