CN114532088A - Tomato grafting wound healing method - Google Patents
Tomato grafting wound healing method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
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- A01G7/045—Electric or magnetic or acoustic treatment of plants for promoting growth with electric lighting
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- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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Abstract
The application relates to the technical field of tomato seedling culture, and particularly discloses a tomato grafting wound healing method. The healing method comprises the following steps: root irrigation is carried out on the seedling scion and the stock before grafting, the seedling scion is grafted to the stock, the grafted seedling is placed at the humidity of 85-95% and the temperature of 18-24 ℃ for wound healing, and a regulator is regularly sprayed to the wound; supplementing light for 4 days after grafting, wherein the intensity of the light supplemented light for the first day is 20-40umol/m2And/s, then gradually increasing the fill light intensity until the fill light intensity is increased to 200umol/m2(s) receiving natural light intensity after 10 days of grafting; and (4) the light supplementing time in the first day is 4h, the light supplementing time is gradually increased after the light supplementing is started, the longest light supplementing time is 12h, and seedling culture is carried out. The tomato grafting wound healing method has the advantage of improving the survival rate of tomato seedlings through the synergistic effect of the steps.
Description
Technical Field
The application relates to the technical field of tomato seedling culture, in particular to a tomato grafting wound healing method.
Background
The tomato is an annual or perennial herbaceous plant, the fruit is oblate or nearly spherical, orange or bright red, the fruit is rich in nutrition and has special flavor, and the tomato can be eaten raw, cooked, processed into tomato sauce or can be stored in a whole fruit can, and the like. Among them, cherry tomatoes are one of the varieties of tomatoes.
The root systems of greenhouse cherry tomato varieties mostly have the defects of weak disease resistance, poor water absorption and the like, so that the scions of the cherry tomatoes need to be grafted to rootstocks with developed root systems and strong disease resistance. The stem of one tomato is grafted onto the stem of another tomato so that the two parts joined together grow into a complete plant, a technique known as tomato seedling grafting.
The grafting method is a prerequisite for survival of tomato seedlings, and the wound healing process after grafting determines the grafting effect and the health condition of the seedling stage. At present, the grafting technology of tomatoes is very common, but the survival rate of grafted tomato seedlings is not high.
Disclosure of Invention
In order to improve the survival rate of grafted tomato seedlings, the application provides a tomato grafting wound healing method.
The application provides a tomato grafting wound healing method, which adopts the following technical scheme:
a tomato grafting wound healing method comprises the following steps:
s1: performing root irrigation on the seedling scion and the stock by using nutrient solution once 48 hours before grafting, grafting the seedling scion to the stock to complete grafting of the seedling scion, placing the grafted seedling at 85-95% humidity and 18-24 ℃ for wound healing, and regularly spraying a regulator to the wound;
s2: supplementing light for 4 days after grafting, wherein the intensity of the light supplemented light for the first day is 20-40umol/m2And/s, then gradually increasing the fill light intensity until the fill light intensity is increased to 200umol/m2(s) receiving natural light intensity after 10 days of grafting; the light supplement time of the first day is 4h, and the light supplement time is gradually increased after the light supplement is started to supplement lightThe light time is 12h at most, and seedling culture is carried out.
By adopting the technical scheme, the tomato grafting wound healing method can promote healing of grafting wounds and improve survival rate of tomato seedlings through synergistic effect of the raw materials of the regulator and synergistic effect of the steps, wherein the survival rate of the tomato seedlings is 89-98%.
Firstly, the scion of the seedling is grafted on the stock, and then the stock is placed under proper humidity and temperature for wound healing, so that the wound can be better healed, and the survival rate of the seedling is improved. The healing of the grafting wound is to control the moisture content in the body, if the moisture of the root is too high, the high root pressure can lead the moisture to be accumulated at the grafting wound, and the healing of the wound is seriously influenced; if the water content of the leaves is too low, the leaves will wilting, and the due physiological function will be lost before the wound is healed, which will affect the health condition of the seedling. The regulator is sprayed to the wound, which is helpful for the wound to heal as soon as possible and can also improve the survival rate of the seedlings. Light intensity also is an important factor of tomato grafting wound healing, and light filling intensity increases gradually, can improve grafting wound's healing effect, if after the light intensity exceeded certain limit, can destroy the activity of grafting healing in-process relevant enzyme, leads to grafting wound tissue browning and necrosis to reduce the healing effect, and then also can influence the survival rate of tomato seedling. The grafted seedling scions are subjected to light supplement, so that the grafted seedling scions can receive enough light, physiological functions of the grafted seedling scions can be promoted, and survival and growth of the seedlings are facilitated.
Preferably, the method comprises the following steps: the regulator comprises the following raw materials in parts by weight: 300 parts of indoleacetic acid 220-one, 30-50 parts of kinetin, 15-28 parts of brassinolide, 8-16 parts of amino-oligosaccharin, 6-15 parts of 6-benzyladenine, 10-18 parts of naphthylacetic acid, 8.8-15 parts of quaternary ammonium salt compound and 1 multiplied by 10 of water5-1×106And (4) portions are obtained.
By adopting the technical scheme, the grafting wound can be promoted to heal through the synergistic effect of the raw materials of the regulator, the growth of cells is promoted, and the survival rate of seedlings is conveniently improved. Specifically, water is used as a solvent, indoleacetic acid is used as a basic component, and the indoleacetic acid can promote the elongation and the differentiation of cells, can induce the expansion of callus, and further promotes the healing of grafting wounds. The kinetin has the function of promoting cell division and can promote the growth of buds of callus. 6-benzyladenine can over-stimulate cell division to cause plant growth and development. Indoleacetic acid belongs to auxin, kinetin and 6-benzyladenine belong to cytokinin, the auxin mainly induces tissue dedifferentiation and root formation, the cytokinin is beneficial to bud generation, and not only can healing of callus be promoted, but also rooting and sprouting of tomatoes can be promoted through synergistic effect of the indoleacetic acid, the kinetin and the 6-benzyladenine, so that the survival rate of tomato seedlings is improved. The brassinolide can promote growth, seedling extraction, seedling protection and seedling strengthening, has an obvious promotion effect on cell division, and can promote healing of grafting wounds. The amino-oligosaccharin has cell activation effect, is helpful for wound recovery, and has root-promoting effect. The naphthylacetic acid has the functions of promoting growth and rooting, promoting the growth of grafted tissues, and promoting wound healing and seedling survival rate. The quaternary ammonium salt compound can also promote the healing of wounds.
Preferably, the method comprises the following steps: the weight ratio of the quaternary ammonium salt compound to the indoleacetic acid is 1: (25-27).
The addition amount of the quaternary ammonium salt compound is too small, and the healing effect on the grafting wound is not obvious; the addition amount of the quaternary ammonium salt compound is too much, so that the grafting wound healing cannot be promoted, the adverse effect on rhizomes, leaves and the like can be generated, and the survival rate of seedlings is further influenced. Through adopting above-mentioned technical scheme, when the weight ratio of quaternary ammonium salt compound and indoleacetic acid is in above-mentioned within range, not only can play the effect of promotion to grafting wound healing, can also improve tomato seedling's survival rate.
Preferably, the method comprises the following steps: the quaternary ammonium salt compound is prepared by the following method: putting N-isobutyl sorbamide and toluene into water, uniformly mixing, adding chlormequat chloride, uniformly mixing, heating to 100 ℃ and 140 ℃, refluxing, performing rotary evaporation to obtain a solid, and drying to obtain the quaternary ammonium salt compound.
Further, the quaternary ammonium salt compound is prepared by the following method: putting N-isobutyl sorbamide and toluene into water, stirring for 30-40min, adding chlormequat chloride, stirring for 10-20min, heating to 140 ℃ at 100 ℃, refluxing for 1-2h, performing rotary evaporation to obtain a solid, and drying to obtain a quaternary ammonium salt compound;
wherein the weight ratio of the N-isobutyl sorbamide to the chlormequat chloride is 1:1, the addition amount of toluene in 1g of the N-isobutyl sorbamide is 4-5mL, and the addition amount of water is 1-2 mL.
By adopting the technical scheme and utilizing the method to prepare the quaternary ammonium salt compound, the prepared quaternary ammonium salt compound can better play a role, and the survival rate of seedlings is convenient to improve.
Preferably, the method comprises the following steps: the regulator in the step S1 is sprayed once every 2-4 days, and the spraying amount of the regulator is 4-5mL4-5 mL/plant, and the spraying is carried out 3-5 times in total.
By adopting the technical scheme, the spraying amount and the spraying frequency of the regulator are limited, the using amount of the regulator is too small, the effect on tomato wounds is not great, and the healing of tomato grafting wounds cannot be promoted well; the excessive consumption of the regulator can affect the physiological functions of the tomatoes, easily burn the tomato seedlings and affect the survival rate of the seedlings.
Preferably, the method comprises the following steps: the fill light intensity of the first day in the step S2 is 20-40umol/m2S, and 20umol/m per day in the first five days after the light supplement is started2The fill-in light intensity is increased at a speed of 40umol/m per day2Increasing the light intensity at the speed of/s until the light intensity is increased to 200umol/m2After/s, receiving natural light intensity; the light supplementing time in the first day is 4h, the light supplementing time is increased at a speed of 1h/d in the first five days after the light supplementing is started, then the light supplementing time is increased at a speed of 2h/d, and the longest light supplementing time is 12 h.
Through adopting above-mentioned technical scheme, further inject light filling intensity and light filling time, help guaranteeing the normal physiological function of tomato seedling, be convenient for promote the healing of grafting wound, be convenient for improve the survival rate of tomato seedling.
Preferably, the method comprises the following steps: the irrigation amount of each seedling scion and rootstock in the step S1 is 39.67-43.67mL respectively.
Through adopting above-mentioned technical scheme, carry out root irrigation to scion and stock two days before the grafting, it is too big to reduce root moisture, otherwise makes moisture pile up in the grafting wound easily, can influence the healing of wound, influences the seedling survival rate.
Preferably, the method comprises the following steps: the nutrient solution comprises the following raw materials in parts by weight: solution A: 24-28 parts of calcium ammonium nitrate, 4.56-6.56 parts of calcium chloride, 0.42 part of Fe-DTPA-110.22 and 165.02-171.22 parts of water; and B, liquid B: 6-8 parts of potassium nitrate, 3.04-5.04 parts of monopotassium phosphate, 16.2-18.2 parts of magnesium sulfate heptahydrate, 6-8 parts of potassium sulfate, 0.15-0.35 part of zinc, 0.15-0.35 part of manganese, 0.118-0.138 part of copper, 0.715-0.915 part of boron, 0.02-0.04 part of molybdenum and 158.967-167.607 parts of water.
Through adopting above-mentioned technical scheme, utilize above-mentioned nutrient solution to irrigate the tomato seedling, provide sufficient nutrition and moisture for the seedling, provide suitable environment for the growth of seedling, be convenient for the survival rate of tomato seedling.
Preferably, the method comprises the following steps: when the light supplement is started in step S2, irrigation is performed together.
Further, in the step S2, the irrigation volume of each tomato seedling on the 0 th to 3 th days after the light supplement is started is 12.89-14.89mL, the irrigation volume of each tomato seedling on the 4 th to 6 th days after the light supplement is started is 25.78-29.78mL, and the irrigation volume of each tomato seedling on the 7 th days after the light supplement is started is 39.67-43.67 mL.
Through adopting above-mentioned technical scheme, slowly increase the irrigation volume in the light filling, for the tomato seedling provides sufficient moisture, promote the growth of seedling, improve the survival rate of seedling.
In summary, the present application includes at least one of the following beneficial technical effects:
1. because the regulator is sprayed on the grafting wound to promote the healing of the wound, the synergistic effect of the regulator on the raw materials promotes cell division and callus expansion, promotes rooting and germination, is convenient to improve the survival rate of the tomato seedlings, and can ensure that the survival rate of the tomato seedlings reaches 98%.
2. The quaternary ammonium salt compound is preferably selected in the application, so that the healing of a grafting wound can be promoted, and the survival rate of tomato seedlings can be further promoted.
Detailed Description
The present application is described in further detail below with reference to specific contents.
Raw materials
Indoleacetic acid is technical grade and has a CAS number of 87-51-4; the molecular weight of the kinetin is 215.21, the CAS number is 525-79-1, and the drying weight loss is less than or equal to 2 percent; brassinolide density of 1.141g/cm3Molecular weight is 480.677, boiling point is 623.7 ℃, flash point is 202.3 ℃, CAS number is 72962-43-7; the molecular weight of the amino-oligosaccharin is 483.37, and the CAS number is 219714-96-2; the melting point of the 6-benzyladenine is 230-232 ℃, the drying weight loss is less than or equal to 0.50 percent, and the CAS number is 1214-39-7; the CAS number of the naphthylacetic acid is 86-87-3; the molecular weight of the N-isobutyl sorbamide is 167.25, and the CAS number is 82240-09-3; the CAS number of chlormequat chloride is 999-81-5; the solubility of Fe-DTPA-11 is 110g/L, the content of iron is more than or equal to 11.1 percent, and the content of chlorine is less than or equal to 0.05 percent; magnesium sulfate heptahydrate has a melting point of 1124 ℃ and a CAS number of 10034-99-8.
Preparation example
Preparation example 1
A quaternary ammonium salt compound is prepared by the following method:
putting N-isobutyl sorbamide and toluene into water, stirring for 35min, adding chlormequat chloride, stirring for 15min, heating to 120 ℃, refluxing for 1.5h, performing rotary evaporation to obtain a solid, and drying to obtain a quaternary ammonium salt compound;
wherein the weight ratio of the N-isobutyl sorbamide to the chlormequat chloride is 1:1, the addition amount of toluene in 1g of the N-isobutyl sorbamide is 4.5mL, and the addition amount of water is 1.5 mL.
Preparation example 2
A regulator prepared by the following method:
indole acetic acid, kinetin, brassinolide, amino-oligosaccharin, 6-benzyl adenine and naphthylacetic acid, and the quaternary ammonium salt compound prepared by the preparation example 1 are put into water and stirred for 40min to obtain the regulator, wherein the raw material ratio is shown in table 1.
Preparation examples 3 to 9
A regulator is different from the regulator prepared in preparation example 2 in the raw material ratio shown in Table 1.
TABLE 1 PREPARATION EXAMPLES 2-9 amount of each material (unit: kg) of the regulators
Preparation example 10
A nutrient solution is prepared by adopting the following method:
mixing 26kg of calcium ammonium nitrate, 5.56kg of calcium chloride, 0.32kg of Fe-DTPA-11 and 168.12kg of water, and stirring for 20min to obtain solution A; mixing 7kg potassium nitrate, 4.04kg potassium dihydrogen phosphate, 17.2kg magnesium sulfate heptahydrate, 7kg potassium sulfate, 0.25kg zinc, 0.25kg manganese, 0.128kg copper, 0.815kg boron, 0.03kg molybdenum and 163.287kg water, and stirring for 1h to obtain solution B; mixing solution A and solution B, and stirring for 30min to obtain nutritional liquid.
Examples
Example 1
A tomato grafting wound healing method comprises the following steps:
s1.1: performing root irrigation on the seedling scion and the rootstock by using the nutrient solution prepared in the preparation example 10 48 hours before grafting, wherein the irrigation volume of each seedling scion and rootstock is 41.67 mL;
s1.2: grafting the seedling scion to the rootstock to complete grafting of the seedling scion, placing the grafted seedling scion at the humidity of 90% and the temperature of 20 ℃ for wound healing, and spraying the regulator prepared in the preparation example 2 to the wound every 3 days, wherein the spraying amount is 4.5 mL/plant for 4 times in total;
s2: and after grafting for 3 days, performing light supplement and irrigation on the grafted seedlings, wherein the specific light supplement intensity, light supplement time and irrigation amount are shown in a table 2, and performing seedling culture.
TABLE 2 light intensity, light supplement time, irrigation amount of grafted supplement
| Days after grafting | 0-3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | After 11 days |
| Fill light intensity (umol/m)2/s) | 0 | 40 | 60 | 80 | 100 | 120 | 160 | 200 | Natural light intensity |
| Time for light supplement (h/d) | 0 | 4 | 5 | 6 | 7 | 8 | 10 | 12 | 12 |
| Irrigation volume (mL/plant) | 0 | 13.89 | 13.89 | 13.89 | 27.78 | 27.78 | 27.78 | 41.67 | 41.67 |
Examples 2 to 8
The tomato grafting wound healing method is different from the tomato grafting wound healing method in the embodiment 1 in that the source of the regulator is different, and the regulator is prepared by the preparation examples 3-9.
Comparative example
Comparative example 1
A tomato grafting wound healing method, which is different from the embodiment 1 in that no regulator is sprayed on the grafted seedlings in the step S1.2.
Comparative example 2
The tomato grafting wound healing method is different from the embodiment 1 in that the quaternary ammonium salt compound is not added in the raw materials of the regulator.
Comparative example 3
A tomato grafting wound healing method, which differs from example 1 in that the humidity in step S1.2 is 70%.
Comparative example 4
A tomato grafting wound healing method, which is different from embodiment 1 in that the light supplement intensity 4 days after grafting in step S2 is 200umol/m2S, and remain unchanged.
Comparative example 5
A tomato grafting wound healing method, which is different from the method in embodiment 1 in that the light supplement time 4 days after grafting in step S2 is 12h and is kept unchanged.
Comparative example 6
A tomato grafting wound healing method, which is different from embodiment 1 in that in step S1.1, the nutrient solution prepared in preparation example 10 is used for carrying out root irrigation once on the seedling scion and the rootstock 2h before grafting.
Comparative example 7
A tomato grafting wound healing method, which is different from example 1 in that root irrigation is not performed before a seedling scion is grafted to a rootstock.
Performance test
7500 seedling scions and stocks with similar growth conditions are respectively taken and randomly divided into 15 groups, grafting and culturing are respectively carried out by the same operator according to the tomato grafting wound healing method in the examples 1-8 and the comparative examples 1-7, the survival rate of the tomato seedlings after 10 days of grafting is observed and recorded, and the detection results are shown in the table 3.
TABLE 3 test results
As can be seen from table 3, the tomato grafting wound healing method of the present application can not only promote healing of grafting wounds, but also improve survival rate of tomato seedlings through synergistic effect between the raw materials of the regulator and synergistic effect between the steps, wherein the survival rate of tomato seedlings is 89-98%.
As can be seen by combining example 1 and comparative examples 1-2, the survival rate of the seedlings in example 1 is 89%, which is superior to that in comparative examples 1-2, and the healing of the wound can be accelerated by spraying the wound with the conditioner, and the survival rate of the tomato seedlings can be further improved by adding the quaternary ammonium salt compound into the conditioner.
As can be seen by combining example 1 and comparative example 3, the survival rate of the seedlings in example 1 is 89%, which is better than that in comparative example 3, and shows that the grafted seedlings are cultured at 90% humidity more suitably, which is beneficial to healing of grafted wounds and is convenient for improving the survival rate of tomato seedlings.
As can be seen by combining the example 1 and the comparative example 4, the survival rate of the seedlings in the example 1 is 89%, which is better than that in the comparative example 4, and indicates that the light supplement intensity is more suitable to be gradually increased, and if the light intensity exceeds a certain limit, the activity of related enzymes in the grafting healing process is damaged, so that the tissue of the grafting wound is browned and necrotized, the healing effect of the grafting wound is reduced, and the survival rate of the tomatoes is also influenced.
Combining the example 1 and the comparative example 5, it can be seen that the survival rate of the seedlings in the example 1 is 89%, which is better than that in the comparative example 5, and the gradual increase of the light supplement time is more suitable, so that the healing of the grafting wound can be promoted, and the survival rate of the tomato seedlings can be improved.
Combining the example 1 and the comparative example 6, the survival rate of the seedlings in the example 1 is 89%, which is better than that in the comparative example 6, and shows that root irrigation of the seedling scions and the rootstocks is more suitable 48h before grafting, the condition of wound ponding caused by high root pressure is reduced, healing of grafting wounds is facilitated, and meanwhile the survival rate of the seedlings is improved.
Combining example 1 and comparative example 7, it can be seen that the survival rate of the seedlings in example 1 is 89%, which is better than that in comparative example 6, and shows that the irrigation of the seedling scions and the rootstocks before grafting is more suitable, the moisture of the tomato seedlings within 3 days after grafting can be provided, the healing of grafting wounds can be promoted, and the survival rate of the tomato seedlings can be improved.
Combining with examples 1-8, it can be seen that the survival rate of the seedlings in example 7 is 98%, which is better than that of other examples, and shows that the weight ratio of the quaternary ammonium salt compound and the indoleacetic acid in example 7 is more suitable, and the mixing amount of other raw materials is also more suitable, so that the healing of the grafting wound can be promoted, and the survival rate of the seedlings can be improved.
The embodiments of the present invention are preferred embodiments of the present application, and the scope of the present application is not limited by the embodiments of the present application, so: all equivalent changes made according to the structure, shape and principle of the present application shall be covered by the protection scope of the present application.
Claims (9)
1. The tomato grafting wound healing method is characterized by comprising the following steps:
s1: performing root irrigation on the seedling scion and the stock by using nutrient solution for one time 48 hours before grafting, then grafting the seedling scion to the stock to complete grafting of the seedling scion, placing the grafted seedling at 85-95% humidity and 18-24 ℃ for wound healing, and regularly spraying a regulator to the wound;
s2: supplementing light for 4 days after grafting, wherein the intensity of the light supplemented light for the first day is 20-40umol/m2And/s, then gradually increasing the fill light intensity until the fill light intensity is increased to 200umol/m2(s) receiving natural light intensity after 10 days of grafting; and (4) the light supplementing time in the first day is 4h, the light supplementing time is gradually increased after the light supplementing is started, the longest light supplementing time is 12h, and seedling culture is carried out.
2. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the regulator comprises the following raw materials in parts by weight: 300 parts of indoleacetic acid 220-one, 30-50 parts of kinetin, 15-28 parts of brassinolide, 8-16 parts of amino-oligosaccharin, 6-15 parts of 6-benzyladenine, 10-18 parts of naphthylacetic acid, 8.8-15 parts of quaternary ammonium salt compound and 1 multiplied by 10 of water5-1×106And (4) portions are obtained.
3. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the weight ratio of the quaternary ammonium salt compound to the indoleacetic acid is 1: (25-27).
4. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the quaternary ammonium salt compound is prepared by the following method: putting N-isobutyl sorbamide and toluene into water, uniformly mixing, adding chlormequat chloride, uniformly mixing, heating to 100 ℃ and 140 ℃, refluxing, performing rotary evaporation to obtain a solid, and drying to obtain the quaternary ammonium salt compound.
5. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the regulator in the step S1 is sprayed once every 2-4 days, and the spraying amount of the regulator is 4-5 mL/plant for 3-5 times.
6. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the fill light intensity of the first day in the step S2 is 20-40umol/m2S, and 20umol/m per day in the first five days after the supplementary lighting is started2The fill-in light intensity is increased at a speed of 40umol/m per day2The light intensity of the supplementary light is increased at the speed of/s until the speed is increased to 200umol/m2After/s, receiving natural light intensity; the light supplementing time of the first day is 4h, the light supplementing time is increased at a speed of 1h/d in the first five days after the light supplementing is started, the light supplementing time is increased at a speed of 2h/d, and the longest light supplementing time is 12 h.
7. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: the irrigation amount of each seedling scion and rootstock in the step S1 is 39.67-43.67mL respectively.
8. The tomato grafting wound healing method according to claim 7, wherein the method comprises the following steps: the nutrient solution comprises the following raw materials in parts by weight: solution A: 24-28 parts of calcium ammonium nitrate, 4.56-6.56 parts of calcium chloride, 0.42 part of Fe-DTPA-110.22 and 165.02-171.22 parts of water; and B, liquid B: 6-8 parts of potassium nitrate, 3.04-5.04 parts of monopotassium phosphate, 16.2-18.2 parts of magnesium sulfate heptahydrate, 6-8 parts of potassium sulfate, 0.15-0.35 part of zinc, 0.15-0.35 part of manganese, 0.118-0.138 part of copper, 0.715-0.915 part of boron, 0.02-0.04 part of molybdenum and 158.967-167.607 parts of water.
9. The tomato grafting wound healing method according to claim 1, wherein the method comprises the following steps: when the light supplement is started in step S2, irrigation is performed together.
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