CN103688975B - Preparation method of nano-antibacterial growth regulator for biological source sprouting vegetable - Google Patents
Preparation method of nano-antibacterial growth regulator for biological source sprouting vegetable Download PDFInfo
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- CN103688975B CN103688975B CN201310745498.XA CN201310745498A CN103688975B CN 103688975 B CN103688975 B CN 103688975B CN 201310745498 A CN201310745498 A CN 201310745498A CN 103688975 B CN103688975 B CN 103688975B
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Abstract
The invention discloses a preparation method of a nanometer regulator, wherein the nanometer preparation obtained through the method uses agricultural streptomycin and cytokinin as raw materials. Not only is the effect of regulating the plant growth of the cytokinin kept, but also a sterilizing effect is achieved, and the synergy of agricultural streptomycin and cytokinin are enhanced.
Description
Technical field
The present invention relates to a kind of preparation method of growth regulator, particularly a kind of preparation method of biogenic shoot vegetable nano-antibacterial growth regulator.
Background technology
Bud dish is a kind of vegetables of new development in recent years, it utilizes the seed, rhizome, branch etc. of crop as propagating materials, under the low light level, thermophilic condition, grow bud seedling, gemmule, tender shoots, young stem or the young tip edible, the health care of its quality fresh and tender, nutritious, pollution-free and tool is got rid of.Bud dish is with short production cycle, productivity effect is high, can form seed 4-8 output doubly, and economic benefit is very remarkable, has boundless development prospect.So research can make its prospect growing better novel agent also just self-evident.
Streptomycin is a kind of antibiotic medical pharmacy, and rough being suitable for prevents and treats crops multiple diseases.Soluble in water, to people and animals' low toxicity.Agricultural streptomycin has systemic action, can prevent and treat various plants bacterium and fungal disease.Be mainly used in spraying, also can do to fill with root and seed soaking sterilization etc.Working concentration is generally no more than 220ppm, in case produce poisoning.
The basic element of cell division has another name called 6-BA, 6-benzyl aminoadenine, it is a kind of auxiliary agent of efficient Promoting plant growth, the positive effect of product is the photosynthetic of enhancing plant, effective quickening plant cell extends, growing of plant, and plant fallen flowers can be reduced, shedding, improve plant fruiting rate, also stenospermocarpy can be formed, also seed can be broken, the dormancy of the plant organs such as stem tuber, stratification, that in plant modifying agent, product is the most short long, disease-resistant, this product improves a kind of novel product specification of crop yield especially. and basic structure has an adenine ring.Cell division natural in plant corpus have zeatin, dihydro zeatin, isopentennyladenine, ribosylzeatin, isopentenyladenosine etc.The position mainly tip of a root that they synthesize in vivo.The basic element of cell division of Prof. Du Yucang, except kinetin, also has 6-benzylaminopurine etc.The most obvious physiological action of the basic element of cell division has two kinds: one to be promote cell division and its differentiation of regulation and control.In tissue cultures, plant organ differentiation in the scale effect of the basic element of cell division and growth hormone, during usual ratio height, is conducive to the differentiation of bud; When ratio is low, be conducive to the differentiation of root.Two is delay protein and chlorophyllous degraded, delay senility.The activity of the various basic element of cell division is variant, such as in the biologic test of growth promoting effects, the natural basic element of cell division is as zeatin, isopentennyladenine, than Prof. Du Yucang the basic element of cell division as 6-benzylaminopurine and kinetin high, and in the biologic test delaying chlorophyll decomposition, the former is high for the latter's specific activity.Be one class promote cell division, induced bud formation and promote its growing plants hormone.Mainly be distributed in and carry out fissional position, as inner in the seed of stem apex, the tip of a root, immature seed, sprouting, the fruit that grows.
And there is no information dissemination as the agriculture streptomycin of the plant growth regulator of outstanding kind and the mixing nanometer formulation of the basic element of cell division.
Summary of the invention
The present invention is the above-mentioned deficiency in order to solve existing for prior art, proposes a kind of preparation method of growth regulator, and the conditioning agent utilizing the method to prepare can regulating plant growth, and has bactericidal action.
Technical solution of the present invention is: a kind of preparation method of biogenic shoot vegetable nano-antibacterial growth regulator, is characterized in that: described method is carried out according to following steps:
The 3ppm cell division cellulose solution of a, the 15ppm agricultural streptomycin solution getting 5 parts of volumes and 1 part of volume adds in beaker, mixed solution pH to 4.7-5.7 in adjustment beaker, the soybean lecithin of the 1.5mg/ml of 1.5 parts of volumes is added in beaker, ice bath magnetic agitation 35-35min under the condition of ultrasound works frequency 80KHz, ultrasonic electric power 500W, 2000r/min
B, the solution after a step process is added the beaker being arranged in ultrasonic cleaning machine, adition process continues 9-10min, then to this solution impulse ultrasound process 30min under the condition of power 85%, On 5s/Off 3s, leave standstill 15min, magnetic agitation 30min, the STPP solution that concentration is 1% is added in beaker, the volume of the STPP solution added is 1.5% of agricultural streptomycin volume, continue impulse ultrasound process 30min, obtain the finely dispersed basic element of cell division-agricultural streptomycin nanometer formulation, i.e. biogenic shoot vegetable nano-antibacterial growth regulator.
Compared with the existing technology, tool has the following advantages in the present invention:
The invention discloses a kind of preparation method of nanometer regulator, utilize the nanometer formulation that this kind of method obtains, adopt agricultural streptomycin and the basic element of cell division as raw material.It not only remains the effect of basic element of cell division regulating plant growth, also has bactericidal action simultaneously, and strengthens the common action effect of both agricultural streptomycin and the basic element of cell division.
Accompanying drawing explanation
Fig. 1 is the photo under 400 times, the microscope of the nanometer regulator nano particle SEM figure utilizing the method for the invention to obtain.
Fig. 2 is the photo under 400 times, same concentration bio-compounding agent former dose of SEM figure microscope.
Fig. 3 be same concentration nanometer regulator (on) with former dose (under) comparison diagram (5 days).
Fig. 4 be same concentration nanometer regulator (on) with former dose (under) comparison diagram (10 days).
Embodiment
The embodiment of the embodiment of the present invention is described below in conjunction with accompanying drawing, as shown in Figures 1 to 4: a kind of preparation method of biogenic shoot vegetable nano-antibacterial growth regulator, carries out according to following steps:
First the agricultural streptomycin solution of 5 parts of volumes and the cell division cellulose solution of 1 part of volume is got, above-mentioned two solution are together added in beaker, then the pH of the mixed solution in beaker is adjusted to 4.7-5.7, the soybean lecithin of the 1.5mg/ml of 1.5 parts of volumes is added as dispersant in beaker, under the condition of ultrasound works frequency 80KHz, ultrasonic electric power 500W, 2000r/min, ice bath magnetic agitation is carried out to the solution after adding dispersant, mixing time is 35-35min
Solution after above-mentioned process is slowly added the beaker that (be preferably and dropwise add) is arranged in ultrasonic cleaning machine, the whole process lasts 9-10min added, then to this solution at power 85%, impulse ultrasound process 30min under the condition of On 5s/Off 3s, 15min is left standstill after process, magnetic agitation 30min again, backward beaker in add the STPP solution that 1ml concentration is 1%, and the volume of the STPP solution added is 1.5% of agricultural streptomycin volume, continue impulse ultrasound process 30min, obtain the finely dispersed basic element of cell division-agricultural streptomycin nanometer formulation, i.e. biogenic shoot vegetable nano-antibacterial growth regulator, this kind of nanometer regulator is suspended emulsion, its particle size is less than 500nm, and be uniformly dispersed.
Embodiment 1
Get raw cell mitogen 3ppm, agricultural streptomycin 15ppm, the two is mixed, and be placed on magnetic stirring apparatus and carry out stirring 30min, put it into after solution mixes in supersonic wave cleaning machine and carry out impulse ultrasound process 30min, obtain the required basic element of cell division-agricultural streptomycin nanometer formulation, i.e. biogenic shoot vegetable nano-antibacterial growth regulator.
The research of biogenic shoot vegetable nano-antibacterial growth regulator is carried out for mung bean.Get the nanometer formulation that appropriate embodiment 1 obtains, with its solution seed soaking 8 hours, then transfer in the middle of culture dish.Wheat seed uses the HgCl of 0.5% in advance
2sterilization.Culture dish is through cleaning and sterilizing, and the absorbent cotton spreading thick about 0.5cm at the bottom of ware is used to keep moisture.By the size chosen, the mung bean seed that form is all identical is put in culture dish, 50, every ware.Soak seed with the formulation soln of corresponding concentration, every ware 20ml, makes seed maintenance moistening and ventilated, guarantees that seed can normally germinate.Repetition like this, 3d observes germination, and 4d adds up germination rate, waters every day afterwards with running water, and drip the basic element of cell division and the agricultural streptomycin mixing nanometer Ji solution 5d of respective concentration every day, 10d measures indices.
Result of study data see the following form:
Table 1 basic element of cell division and agricultural streptomycin mixing nanometer formulation and former dose cultivate lower mung bean sprouts germination rate
Table one illustrates, mung bean germination vigor after chemicals treatment is better than clear water treatment effect, and after the seed soaking of variable concentrations culture fluid, former dose of 15ppm mung bean sprouts germination vigor and rooting rate best, nanometer formulation is also that 15ppm mung bean sprouts germination vigor and rooting rate are best, improve 3.75% than with concentration former dose of germination rate, rooting rate improves 4.75% than with former dose of concentration.Wherein 10ppm, 15ppm nanometer formulation promotes that mung bean germination rate is the highest, and seed germination effect is best.
Table 2 basic element of cell division and agricultural streptomycin mixing nanometer formulation and former dose cultivate the lower every physical signs of mung bean sprouts
Table two illustrates, after former dose of the solution of variable concentrations and nanometer formulation process mung bean seed, former dose of mung bean sprouts growing way under 10ppm concentration is better, is secondly 15ppm, but is all better than clear water control group.Wherein former dose of the obvious excellent variable concentrations of 5ppm nanometer formulation growing way, illustrates that this medicament nanometer formulation not only can promote the growth of mung bean sprouts, can also effectively improve absorption efficiency and availability, saves medication.
The table bilobular plant number of-3 growth regulation (the 10th day)
Table three illustrates that the growth of use to the night of shoot vegetable of agricultural streptomycin and basic element of cell division mixing nanometer formulation antimicrobial growth conditioning agent has facilitation, and former dose is 10ppm with the optimum concentration of nanometer formulation, but the facilitation of nanometer formulation is obviously better than former dose,
Note: the fresh weight of seedling is cultivation after 5 days, removes endosperm, uses electronic balance weighing gained; The fresh weight of root is also that cultivation weighs for 5 days afterwards
Experiment conclusion: agricultural streptomycin and the basic element of cell division mixing nanometer formulation effect mung bean seed of seeing variable concentrations embodiment from table 1 is less on the germination rate impact of mung bean seed; From table 2, when concentration is lower than 2ppm, with the solution seed soaking of agricultural streptomycin and basic element of cell division mixing nanometer formulation, the fresh weight of its seedling, the fresh weight of root are all better than untreated agricultural streptomycin and the process of basic element of cell division mixed solution, and when 20ppm, show the suppression to root.
Claims (1)
1. a preparation method for biogenic shoot vegetable nano-antibacterial growth regulator, is characterized in that: described method is carried out according to following steps:
The 3ppm cell division cellulose solution of a, the 15ppm agricultural streptomycin solution getting 5 parts of volumes and 1 part of volume adds in beaker, mixed solution pH to 4.7-5.7 in adjustment beaker, the soybean lecithin of the 1.5mg/ml of 1.5 parts of volumes is added in beaker, ice bath magnetic agitation 35min under the condition of ultrasound works frequency 80KHz, ultrasonic electric power 500W, 2000r/min
B, the solution after a step process is added the beaker being arranged in ultrasonic cleaning machine, adition process continues 9-10min, then to this solution impulse ultrasound process 30min under the condition of power 85%, On 5s/Off 3s, leave standstill 15min, magnetic agitation 30min, the STPP solution that concentration is 1% is added in beaker, the volume of the STPP solution added is 1.5% of agricultural streptomycin volume, continue impulse ultrasound process 30min, obtain the finely dispersed basic element of cell division-agricultural streptomycin nanometer formulation, i.e. biogenic shoot vegetable nano-antibacterial growth regulator.
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| CN101990904A (en) * | 2009-08-28 | 2011-03-30 | 霍智彬 | Plant regulator |
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| 细胞分裂素在蔬菜上的应用;李云乐;《农业技术与装备》;20121231(第238期);第45－46页 * |
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