CN105011020A - Wheat seedling powder high in biological selenium content and preparation method thereof - Google Patents
Wheat seedling powder high in biological selenium content and preparation method thereof Download PDFInfo
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- CN105011020A CN105011020A CN201510474448.1A CN201510474448A CN105011020A CN 105011020 A CN105011020 A CN 105011020A CN 201510474448 A CN201510474448 A CN 201510474448A CN 105011020 A CN105011020 A CN 105011020A
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- wheat seedling
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- 239000011669 selenium Substances 0.000 title claims abstract description 120
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 110
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 110
- 241000209140 Triticum Species 0.000 title claims abstract description 53
- 235000021307 Triticum Nutrition 0.000 title claims abstract description 49
- 239000000843 powder Substances 0.000 title claims abstract description 34
- 238000002360 preparation method Methods 0.000 title claims abstract description 8
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 12
- 241001668545 Pascopyrum Species 0.000 claims description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000008367 deionised water Substances 0.000 claims description 6
- 229910021641 deionized water Inorganic materials 0.000 claims description 6
- 238000010899 nucleation Methods 0.000 claims description 6
- 230000008859 change Effects 0.000 claims description 5
- 239000000047 product Substances 0.000 claims description 5
- 235000013339 cereals Nutrition 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 3
- 230000003203 everyday effect Effects 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- 230000035784 germination Effects 0.000 claims description 3
- 239000012535 impurity Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000007921 spray Substances 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 239000013589 supplement Substances 0.000 claims description 3
- 238000005406 washing Methods 0.000 claims description 3
- 230000004071 biological effect Effects 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 abstract description 2
- 230000009466 transformation Effects 0.000 abstract 1
- 244000098338 Triticum aestivum Species 0.000 description 8
- 235000016709 nutrition Nutrition 0.000 description 4
- 230000006870 function Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 206010020772 Hypertension Diseases 0.000 description 2
- 102000008114 Selenoproteins Human genes 0.000 description 2
- 108010074686 Selenoproteins Proteins 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 230000031018 biological processes and functions Effects 0.000 description 2
- 230000000875 corresponding effect Effects 0.000 description 2
- 238000000502 dialysis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000002372 labelling Methods 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 208000009525 Myocarditis Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 208000034189 Sclerosis Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000002605 anti-dotal effect Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000017531 blood circulation Effects 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 208000029078 coronary artery disease Diseases 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001506 fluorescence spectroscopy Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 230000031891 intestinal absorption Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 230000003449 preventive effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 150000003346 selenoethers Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention belongs to the technical field of bioengineering and particularly relates to wheat seedling powder high in biological selenium content and a preparation method thereof. According to the wheat seedling powder high in biological selenium content and the preparation method thereof, wheat is cultivated by utilizing selenium-enriched dried yeast, under the secondary transformation by the wheat,selenium which is contained in organic selenium and not bound to the molecular structure is transformed into biological selenium which is greater in biological effect, and the wheat seedling powder high in biological selenium content is produced. According to an obtained wheat seedling powder product rich in selenium, the biological selenium content reaches as high as 95-98%, the content of other nutrient elements in the wheat seedling powder is also increased by different degrees, and the content of the biological selenium in wheat seedling can be controlled autonomously so that it can be guaranteed that the physiological needs of human bodies are met.
Description
Technical field
The invention belongs to technical field of bioengineering, be specifically related to a kind of high Biological Selenium content Wheat Grass Powder and preparation method thereof.
Technical background
Selenium is the trace element of needed by human, and its biological effect is also multiaspect, and research shows, selenium significantly can reduce the M & M of kinds cancer, has good treatment and preventive effect; Selenium can also maintain normal configuration and the function of cardio-cerebrovascular, be conducive to the normal level maintaining blood pressure, and having regulating action to the hypertension that a variety of causes causes, selenium has repair to blood vessel, thus coronary heart disease, hypertension, artery sclerosis and myocarditis is had to the effect of prevention and therapy; Selenium or a kind of antioxidant eating source property, harmful free radicals in energy purged body also prevents vivo oxidation from damaging, and play antidotal effect, selenium also has the function strengthening immunity simultaneously.
A lot of biological functions of selenium are realized, so the selenium of not all form all has similar function by the synthesis of functional protein in participation body.Inorganic selenium may be reduced to insoluble or not easily absorbed selenides by a certain mode before entering small intestine, the selenium of this form would not by intestinal absorption, directly discharge from ight soil, and inorganic selenium toxicity is comparatively large, a lot of country has forbidden inorganic selenium to use as nutrition fortifier.And after Organic Selenium enters human body, first corresponding amino acid selenium can be converted to by small intestine, and then absorbed with the form of single amino acid selenium, selenium after absorption is transported to rapidly liver through blood circulation and is combined with selenoprotein, or be directly transported to body tissue and the protein bound in tissue, finally with the formal distribution of selenoprotein in different histocytes, and in Organic Selenium, really be attached to the Biological Selenium on structure of biological macromolecule, biological action is the strongest.Current organic selenium product difference in quality is on the market large, do not reach corresponding effect, main cause is exactly that part inorganic selenium is attached to molecular surface with physical enrichment effect and is not really attached in the structure of large biological molecule, causes the content of Biological Selenium in the Organic Selenium prepared lower.
Summary of the invention
The present invention, in order to solve the lower problem of current rich selenium product Biological Selenium content on the market, provides a kind of method being transformed the high Biological Selenium content Wheat Grass Powder of preparation by two secondary pollutants.
High Biological Selenium content Wheat Grass Powder of the present invention utilizes rich selenium dry ferment to soak to raise wheat No. 5 Wheat Species, through raising the secondary conversion of wheat No. 5 wheat seedlings, the selenium be not attached in Organic Selenium on molecular structure is changed into Biological Selenium, raises Biological Selenium content in wheat No. 5 Wheat Grass Powder products and reach 95-98%.
The object of the invention technical scheme:
1, Se-enriched yeast
The present invention's Se-enriched yeast used is provided by Angel Yeast Co., Ltd.
2, wheat seeds domestication
Selecting different wheat wheat seeds, different condition of culture is set and cultivates respectively, grow to after certain altitude until wheat seeding and cut from root, dry, pulverize, measure its rich selenium amount, determining that wheat seeds that rich selenium amount is the highest are for raising wheat No. 5.
3, cultivate
Take the rich selenium dry ferment of 36-55g, add 1L deionized water, be mixed with the solution of selenium concentration 40-60 μ g/mL, wheat seeds pick removing impurity and not full grain, take the wheat seeds of 1-2kg, sterilize and use aseptic water washing, then wheat seeds 4-6 hour is soaked with the yeast selenium solution prepared, treat that wheat seeds absorb sufficient moisture, taking out to load is lined with in the disk of wet gauze, cover with straw screen or mat, seed is allowed to germinate at the temperature of 15-20 DEG C, duration of germination deionized water supplements the moisture germinateing and consume, but be no more than 1/2 of seed height, illumination cultivation room is moved into after 1-2 days, add in disk prepare containing selenium complete culture solution, change every day and contain selenium complete culture solution 2-3 time, and carry out a blade face with the yeast selenium solution identical with seed soaking concentration and execute spray, cut from root when wheat seeding grows to 15-20cm height, after freeze drying, pulverize, be high Biological Selenium content Wheat Grass Powder.
The present invention compared with prior art its beneficial effect is: the second time biology through wheat transforms, after being transformed first time, in Organic Selenium, the selenium be not really attached on molecular structure is not converted into real Biological Selenium, the Se-rich wheat seedling product Biological Selenium content obtained is up to 98%, and the content of other nutrients also has increase in various degree in wheat seedling, and the content of Biological Selenium in the wheat seedling that the present invention can also produce from main control, to ensure the physiological requirements meeting human body.
Detailed description of the invention
The preparation method of embodiment 1 high Biological Selenium content Wheat Grass Powder and with the contrast not adding the common wheat seedling powder that selenium solution is cultivated:
(1) take the rich selenium dry ferment of 36-55g, add 1L deionized water, stir and be mixed with the solution of selenium concentration 40-60 μ g/mL;
(2) wheat No. 5 wheat seeds will be raised and pick removal of impurities and not full grain, take the wheat seeds of 1-2kg, sterilize and use aseptic water washing, wheat seeds 4-6 hour is soaked with the yeast selenium solution prepared, treat that wheat seeds absorb sufficient moisture, taking out to load is lined with in the disk of wet gauze, cover with straw screen or mat, shading treatment, and allow seed germinate at 15-20 DEG C, duration of germination deionized water supplements the moisture germinateing and consume, but be no more than 1/2 of seed height, illumination cultivation room is moved into after 1-2 days, add in disk prepare containing selenium complete culture solution, change every day and contain selenium complete culture solution 2-3 time, and with the yeast selenium solution identical with seed soaking concentration, carry out a blade face and execute spray, cut from root when wheat seeding grows to 15-20cm, freeze drying, pulverize, obtain Se-rich wheat seedling powder.
(3) cultivate with the identical Wheat Species complete culture solution not adding selenium, prepare common wheat seedling powder in contrast simultaneously.
The present invention's complete culture solution compound method used is get the K of 1mL1M
2h
2pO
4, 5mL1M KNO
3, 5mL1M Ca (NO
3)
2, 2mL1M MgSO
4adding distil water constant volume to 1L, then adds the 2.86g/L H of each 1mL in the solution prepared
3bO
2, 1.61g/L MnCL
24H
2o, 0.22g/L ZnSO
47H
2o, 0.08g/L CuSO
45H
2o, 0.02g/L H
2mnO
4h
2o, and 1mL FeEDTA solution.
Containing selenium complete culture solution namely on the basis of above-mentioned complete culture solution, add yeast selenium dry powder, make selenium concentration be 40-60 μ g/mL.
The mensuration of total Se content adopts the second method fluorescence spectrometry of regulation in GB5009.93-2010 " mensuration of Selenium in Food ".
The assay method of organic selenium content removes inorganic selenium for adopting biomembrane dialysis, namely measures inorganic selenium content in dislysate at set intervals, until inorganic selenium content does not increase in dislysate, namely dialyses complete, measure organic selenium content after dialysis.
The assay method of Biological Selenium content goes out Biological Selenium for adopting high performance liquid chromatography extraction and isolation, measures its content.
Measurement result is in Table 1-3:
Total selenium, Organic Selenium, Biological Selenium content in table 1 Se-enriched yeast powder
Total selenium, Organic Selenium, Biological Selenium content in table 2 common wheat seedling powder
Total selenium, Organic Selenium, Biological Selenium content in table 3 high Biological Selenium content Wheat Grass Powder
From table 1,2,3, in the Wheat Grass Powder of high Biological Selenium content prepared by the present invention, the content of Organic Selenium and Biological Selenium is greatly improved compared with common wheat seeding, and the conversion ratio of Organic Selenium, Biological Selenium is significantly improved compared with Se-enriched yeast.
The change of embodiment 2 Se-rich wheat seedling powder nutritional labeling compared with common wheat seedling powder
The present invention by analyze measure, compare add selenium cultivate rich selenium wheat seedling powder and hydroponic common wheat seedling powder nutrient content change.The results are shown in Table 4
Table 4 Se-rich wheat seedling powder every 100g Middle nutrition cellulose content compared with common wheat seedling powder changes
From above data, wheat is cultivated with Organic Selenium, transformed by two secondary pollutants of wheat seeding, the Wheat Grass Powder cultivated not only is rich in the Biological Selenium that human absorptivity is higher, biological action is strong, and in Wheat Grass Powder, other nutritional labeling also has increase in various degree compared with common wheat seedling powder.
Claims (2)
1. one kind high Biological Selenium content Wheat Grass Powder, it is characterized in that utilizing rich selenium dry ferment to soak raises wheat No. 5 Wheat Species, through raising the secondary conversion of wheat No. 5 wheat seedlings, the selenium be not attached in Organic Selenium on molecular structure is changed into Biological Selenium, raises Biological Selenium content in wheat No. 5 Wheat Grass Powder products and reach 95-98%.
2. the preparation method of a kind of high Biological Selenium content Wheat Grass Powder according to claim 1, is characterized in that being made up of following steps:
(1) take the rich selenium dry ferment of 36-55g, add 1L deionized water, be mixed with the solution of selenium concentration 40-60 μ g/mL:
(2) wheat No. 5 wheat seeds will be raised and pick removal of impurities and not full grain, and take the wheat seeds of 1-2kg, sterilize and use aseptic water washing:
(3) wheat seeds 4-6 hour is soaked with the yeast selenium solution prepared, treat that wheat seeds absorb sufficient moisture, taking out to load is lined with in the disk of wet gauze, cover with straw screen or mat, seed is allowed to germinate at 15-20 DEG C of temperature, duration of germination deionized water supplements the moisture germinateing and consume, but be no more than 1/2 of seed height, within 1-2 days, add in backward disk prepare containing selenium complete culture solution, change every day and contain selenium complete culture solution 2-3 time, and carry out a blade face with the yeast selenium solution identical with seed soaking concentration and execute spray, cut from root when wheat seeding grows to 15-20cm, freeze drying, pulverize, obtain Se-rich wheat seedling powder.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510474448.1A CN105011020A (en) | 2015-08-05 | 2015-08-05 | Wheat seedling powder high in biological selenium content and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510474448.1A CN105011020A (en) | 2015-08-05 | 2015-08-05 | Wheat seedling powder high in biological selenium content and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN105011020A true CN105011020A (en) | 2015-11-04 |
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| CN201510474448.1A Pending CN105011020A (en) | 2015-08-05 | 2015-08-05 | Wheat seedling powder high in biological selenium content and preparation method thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258454A (en) * | 2016-09-29 | 2017-01-04 | 印天寿 | A kind of family oriented produces the method for wheat seedling rich in selenium powder |
| CN106489512A (en) * | 2016-11-30 | 2017-03-15 | 广州市瑞硒康生物科技有限公司 | A kind of inoculation method of selenium-rich wheat seeding and the method for preparing selenium-rich wheat seedling powder |
| CN107495385A (en) * | 2017-08-04 | 2017-12-22 | 陕西博硒农业科技有限公司 | A kind of selenium-rich food additive and preparation method thereof |
| CN107950895A (en) * | 2017-12-25 | 2018-04-24 | 安徽过湾农业科技有限公司 | A kind of production method of organic selenium-rich cereal bean sprout freeze-dried powder |
| CN109122126A (en) * | 2018-08-10 | 2019-01-04 | 安徽硒无忧现代农业科技有限公司 | A kind of preparation process of product containing organic selenium powder |
| CN112155200A (en) * | 2020-09-16 | 2021-01-01 | 合肥中科启奥生物科技有限公司 | Preparation method of selenium-rich barley green juice powder |
| CN116369205A (en) * | 2023-04-24 | 2023-07-04 | 安新县益农农业科技有限公司 | Selenium-enriched wheat breeding method |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106258454A (en) * | 2016-09-29 | 2017-01-04 | 印天寿 | A kind of family oriented produces the method for wheat seedling rich in selenium powder |
| CN106489512A (en) * | 2016-11-30 | 2017-03-15 | 广州市瑞硒康生物科技有限公司 | A kind of inoculation method of selenium-rich wheat seeding and the method for preparing selenium-rich wheat seedling powder |
| CN107495385A (en) * | 2017-08-04 | 2017-12-22 | 陕西博硒农业科技有限公司 | A kind of selenium-rich food additive and preparation method thereof |
| CN107950895A (en) * | 2017-12-25 | 2018-04-24 | 安徽过湾农业科技有限公司 | A kind of production method of organic selenium-rich cereal bean sprout freeze-dried powder |
| CN109122126A (en) * | 2018-08-10 | 2019-01-04 | 安徽硒无忧现代农业科技有限公司 | A kind of preparation process of product containing organic selenium powder |
| CN112155200A (en) * | 2020-09-16 | 2021-01-01 | 合肥中科启奥生物科技有限公司 | Preparation method of selenium-rich barley green juice powder |
| CN116369205A (en) * | 2023-04-24 | 2023-07-04 | 安新县益农农业科技有限公司 | Selenium-enriched wheat breeding method |
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Application publication date: 20151104 |