CN110432288B - Application of chafer metarhizium anisopliae microspore variety CQMa421 in preparation of medicament for preventing and treating bud rot disease - Google Patents
Application of chafer metarhizium anisopliae microspore variety CQMa421 in preparation of medicament for preventing and treating bud rot disease Download PDFInfo
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Abstract
The invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to an application of metarhizium anisopliae microsporum CQMa421 in preparation of a medicament for preventing and treating bean sprout rot. The strain is a chafer metarhizium anisopliae microsporum CQMa421 strain, has a good prevention and treatment effect on the rot disease of the sprouts, and can remarkably promote the growth of the sprouts.
Description
Technical Field
The invention belongs to the technical field of biocides containing microbial fungi, and particularly relates to an application of metarhizium anisopliae microsporum CQMa421 in preparation of a medicament for preventing and treating bean sprout rot.
Background
Edible sprouts, bud balls, tender buds and young stem and young tips can be directly grown by utilizing plant seeds or other nutritional organs (rhizomes, branches and the like) under dark and weak light (or no shading), and are called sprout vegetables for short (common problems and countermeasures in sprout vegetable production, high delicacy, modern agriculture, 12 th stage 2012, page 8, lines 1-3 and 12/31/2012).
The bean sprouts are also named as sprouting vegetables, smart sprouts, bean sprouts, ruyi vegetables, jia vegetables, silver sprouts, silver needles, silver seedlings, sprout cores, soybean sprouts, bean sprouts in clear water and the like, and edible 'sprouts' are cultivated from seeds of various cereals, beans and trees and are also called 'living vegetables'. The bean sprouts are rich in variety and nutrition and are common vegetables, and the common bean sprouts comprise soybean sprouts, mung bean sprouts, peanut sprouts, Chinese toon sprouts, broad bean sprouts and the like. The sprouts contain various nutrient elements necessary for human body, various necessary amino acids decomposed from protein, and because the sprouts have moderate price and unlimited growing season, the sprouts are more and more favored by consumers.
However, diseases such as root rot, mildew spot and insect interception easily occur in the process of producing the bean sprouts. The conventional onset time of the bud disease generally occurs after the second day of the germination period. After the third day of the disease, the disease gradually spreads and expands, and the disease becomes more serious day by day. If the condition is discovered in time after the occurrence of the disease, the failure of taking measures and preventing can cause destructive cylinder reversing and failure, thus causing economic loss. The bud diseases mainly comprise ripe bud diseases (also known as scald bud diseases), red root diseases (also known as bean sprout common cold diseases), rootlet diseases, lumbar fold diseases, root rot diseases, brown spot diseases and the like (the bean sprout disease control, Liu Gong, rural practical technology and information, at the 7 th stage in 2000, the 1 st section of the left column on page 26, the 1 st to 6 th lines of the left column on page 26, the 3 rd section of the left column on page 26, the 1 st line to the 4 th section of page 27, and the 31 th 12 month in 2000.
Disclosure of Invention
In view of the above, the present invention aims to provide an application of a metarhizium anisopliae microsporum CQMa421 strain in preparing a medicament for preventing and treating a bean sprout rot disease.
In order to achieve the purpose, the technical scheme of the invention is as follows:
application of metarhizium anisopliae microsporum CQMa421 strain in preparing medicament for preventing and treating bud rot disease is provided.
The control refers to the ability of a substance to reduce or prevent pathogenic microorganisms from harming crops.
The bean sprouts are also named as sprouting vegetables, smart sprouts, bean sprouts, ruyi vegetables, jia vegetables, silver sprouts, silver needles, silver seedlings, sprout cores, soybean sprouts, clear water bean sprouts and the like, and edible bean sprouts can be cultivated from seeds of various cereals, beans and trees.
The rot disease refers to a disease that the lower part of the hypocotyl or the upper part of the radicle of the bean sprouts start to rot due to germ infection under the conditions of germ carrying, incomplete disinfection, inappropriate growth conditions and the like of bean seeds, and the bean sprouts in the whole container are rotten when the disease is serious.
The inventor unexpectedly discovers that the metarhizium anisopliae microsporum CQMa421 strain has good control effect on the rotten diseases of the sprouts in the research process.
Further, the concentration of the Metarhizium anisopliae microspore variety is 2X 106-2×107Chafer greenThe spore/ml of the small spore variant CQMa of the stiff fungus is 421.
The second purpose of the invention is to protect the application of the metarhizium anisopliae microsporum CQMa421 strain in preparing the medicament for promoting the growth of the bud vegetables.
The inventor unexpectedly discovers that the metarhizium anisopliae microsporum CQMa421 strain can obviously promote the growth of sprouts in the research process.
Further, the concentration of the Metarhizium anisopliae microspore variety is 2X 106-2×107Metarhizium anisopliae microspore variety CQMa421 spore/ml.
The invention also aims to provide a production method for protecting sprouts, which comprises the steps of seed selection, seed soaking, cultivation and harvesting, wherein the seed soaking is to soak seeds of the sprouts by using a metarhizium anisopliae microsporum CQMa421 spore suspension.
Further, spraying the bud vegetable seeds with a chafer green muscardine fungus microspore CQMa421 spore suspension in the cultivation process.
Further, the concentration of Metarhizium anisopliae var minimalis in the spore suspension is 2X 106-2×107Metarhizium anisopliae subsp.minitans CQMa421 spore/ml spore suspension.
Further, the seed soaking time is 20-24 hours.
Further, the temperature during the incubation is 20-25 ℃.
Further, the spraying frequency is 3-5 times per day.
The invention has the beneficial effects that:
the bacterial strain has good prevention and treatment effect on the rot disease of the bean sprouts.
The strain can obviously promote the growth of the bean sprouts.
Detailed Description
The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art.
Example 1
The method for producing soybean sprouts by using the metarhizium anisopliae microspore variety CQMa421 comprises the following specific steps:
s1 seed selection: selecting full soybean seeds with high maturity and no moth impurities, and dividing the soybean seeds into two groups randomly, wherein one group is an experimental group, and the other group is a control group;
s2 preparation of spore suspension: using 25 ℃ civil tap water to respectively dilute 80 hundred million spores/ml metarhizium anisopliae small spore variety CQMa421 oil suspending agent (strain preservation number: CGMCC N0.4609, preparation manufacturer: Chongqing Jurisxin biology Co., Ltd.) by 400 and 4000 times to prepare spore suspensions with the concentrations of 2 x 107 and 2 x 106 spores/ml;
s3 seed soaking: transfer the experimental group soybean seeds to 2X 107Soaking Metarhizium anisopliae microspore CQMa421 spore/ml spore suspension for 24 hours; soaking the soybean seeds of the control group in tap water for 24 hours;
s4 culture: transferring soaked semen glycines seed to cleaned and sterilized sprout tray or sprout container, maintaining temperature at 20-25 deg.C, and using 2 × 10 of experimental group per day6Spraying 4 times of spore suspension of Metarhizium anisopliae microsporum CQMa421 spore/ml; spraying tap water to the control group, wherein the spraying water amount and the spraying frequency of the control group are the same as those of the experimental group;
and S5 harvesting: after culturing for 6 days, detecting the growth condition of bacteria and the weight of hundreds of bean sprouts, and observing whether the bean sprouts are rotten or not, wherein the results are shown in table 1;
the method for detecting the growth condition of the bacteria comprises the following steps: randomly weighing a certain weight of (M1) bean sprouts, transferring the bean sprouts into a certain volume of (V1) 0.85 wt% NaCl solution, uniformly stirring, continuously diluting the uniformly mixed solution by 10 times, coating 50ul of the uniformly mixed solution on an LB solid culture medium flat plate, and performing culture counting (selecting a flat plate for counting about 200 bacterial colonies for calculation, wherein the bacterial colony number x is 20 multiplied by x y multiplied by V1/M1 per gram of bean sprouts, and taking the average value of three times of repetition;
the method for measuring the weight of hundreds of bean sprouts comprises the following steps: randomly selecting 100 bean sprouts, weighing to obtain the weight of hundreds of bean sprouts, and taking the average value of the three repetitions.
TABLE 1 test results
| Source | Bacteria count/cFU | Weight/g of hundred bean sprouts | Whether or not rot occurs |
| Experimental group | 2.35×103 | 96.82 | Is not present |
| Control group | 1.92×108 | 81.93 | Obvious rot condition appears |
As can be seen from Table 1, the number of bacteria in the experimental group was significantly lower than that in the control group after 6 days of culture; compared with the control group, the weight of the hundreds of bean sprouts in the experimental group is improved by 17.17 percent. Therefore, the chafer metarhizium anisopliae microsporum CQMa421 strain can obviously reduce the quantity of the bean sprouts, has good prevention and treatment effect on the rotten diseases of the bean sprouts, and can obviously promote the growth of the bean sprouts.
Example 2
The method for producing the mung bean sprouts by using the metarhizium anisopliae microspore variety CQMa421 comprises the following specific steps:
s1 seed selection: selecting full mung bean seeds with high maturity and no moth impurities, and dividing the mung bean seeds into two groups randomly, wherein one group is an experimental group, and the other group is a control group;
s2 preparation of spore suspension: 80 hundred million spores/ml metarhizium anisopliae small spore variety CQMa421 oil suspending agent (strain preservation number: CGMCC NO.4609, preparation manufacturer: Chongqing Polyricin organism Co., Ltd.) is diluted 400 times and 4000 times respectively by 30 ℃ civil tap water to prepare 2 x 107And 2X 106Spore/ml spore suspension;
s3 seed soaking: transfer the experimental group of mung bean seeds to 2X 107Soaking Metarhizium anisopliae microspore CQMa421 spore/ml spore suspension for 24 hours; soaking the seeds of the control group in tap water for 24 hours;
s4 cultivation: transferring the soaked seeds to a cleaned and disinfected sprout tray or a sprout growing container, keeping the temperature at 20-25 ℃, and spraying the mung bean seeds of the experimental group for 4 times by using 2 × 106 metarhizium anisopliae microspore variant CQMa421 spore/ml spore suspension every day; spraying the mung bean seeds of the control group by using tap water, wherein the spraying water amount and the spraying frequency of the control group are the same as those of the experimental group;
and S5 harvesting: after culturing for 6 days, detecting the growth condition of bacteria and the weight of hundreds of bean sprouts, and observing whether the bean sprouts are rotten or not, wherein the results are shown in table 2;
the method for detecting the growth condition of the bacteria comprises the following steps: randomly weighing a certain weight (M1) of bean sprouts, transferring the bean sprouts into a certain volume (V1) of 0.85% NaCl solution, uniformly stirring, continuously diluting the uniformly mixed solution by 10 times, coating 50ul of the uniformly mixed solution on an LB solid culture medium flat plate, and performing culture counting (selecting a flat plate for counting about 200 bacterial colonies for calculation, wherein the bacterial colony number is x, and the dilution multiple is y). The number of bacteria per gram of bean sprouts is 20 XxyYXV 1/M1, and the results are averaged in triplicate.
The method for measuring the weight of hundreds of bean sprouts comprises the following steps: randomly selecting 100 bean sprouts, weighing to obtain the weight of hundreds of bean sprouts, and taking the average value of the three repetitions.
TABLE 2 test results
| Source | Bacteria count/cFU | Weight/g of hundred bean sprouts | Whether or not rot occurs |
| Experimental group | 4.55×102 | 57.77 | Is not present |
| Control group | 8.82×106 | 37.11 | Obvious rot condition appears |
As can be seen from Table 2, after 6 days of culture, the number of bacteria in the experimental group was significantly lower than that in the control group; compared with the control group, the weight of the hundreds of bean sprouts in the experimental group is increased by 55.67%. Therefore, the chafer metarhizium anisopliae microsporum CQMa421 strain can obviously reduce the quantity of the bean sprouts, has good prevention and treatment effect on the rotten diseases of the bean sprouts, and can obviously promote the growth of the bean sprouts.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (6)
1. Metarhizium anisopliae var minimalis (A. chafer)Metarhizium anisopli aevar.anisopliae) Application of CQMa421 strain in preparation of medicament for promoting growth of bean sprouts, wherein concentration of metarhizium anisopliae microspore variant in medicament is 2 x 106 -2×10 7Spores/ml;
the preservation number of the metarhizium anisopliae microspore variety CQMa421 is CGMCC NO. 4609.
2. The production method for promoting the growth of the bean sprouts is characterized by comprising the steps of seed selection, seed soaking, cultivation and harvesting, wherein the seed soaking is to soak the bean sprouts seeds by using a metarhizium anisopliae microsporum CQMa421 spore suspension, the bean sprouts seeds are sprayed by using the metarhizium anisopliae microsporum CQMa421 spore suspension in the cultivation process, and the concentration of the metarhizium anisopliae microsporum in the spore suspension is 2 x 106 -2×10 7Spore/ml spore suspension;
the preservation number of the metarhizium anisopliae microspore variety CQMa421 is CGMCC NO. 4609.
3. The method of claim 2, wherein the seed soaking time is 20 to 24 hours.
4. The method for producing according to claim 2 or 3, wherein the temperature during the incubation is 20 to 25 ℃.
5. The production method according to claim 2 or 3, wherein the spraying frequency is 3 to 5 times/day.
6. The production method according to claim 4, wherein the spraying frequency is 3 to 5 times per day.
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|---|---|---|---|---|
| CN103688975A (en) * | 2013-12-31 | 2014-04-02 | 辽宁师范大学 | Preparation method of nano-antibacterial growth regulator for biological source sprouting vegetable |
| CN104694395A (en) * | 2013-12-09 | 2015-06-10 | 河北农业大学 | Metarhizium anisopliae with peanut fruit rot prevention effect, and fermentation process thereof |
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| KR20160139521A (en) * | 2015-05-28 | 2016-12-07 | 충북대학교 산학협력단 | Entomopathogenic fungi Metarhizium anisopliae SD4-2 and Beauveria bassiana SD15 having antimicrobial activities and insectcide |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104694395A (en) * | 2013-12-09 | 2015-06-10 | 河北农业大学 | Metarhizium anisopliae with peanut fruit rot prevention effect, and fermentation process thereof |
| CN103688975A (en) * | 2013-12-31 | 2014-04-02 | 辽宁师范大学 | Preparation method of nano-antibacterial growth regulator for biological source sprouting vegetable |
Non-Patent Citations (4)
| Title |
|---|
| 2016年登记的新农药品种简介;刘刚;《农药市场信息》;20170228(第3期);第37页左栏 * |
| 4种绿僵菌乙酸乙酯初提物抗菌活性比较;华梅 等;《西部林业科学》;20180430;第47卷(第2期);第67页表1 * |
| New antibacterial isocoumarin glycosides from a wetland soil derived fungal strain Metarhizium anisopliae;Tian, JF et al.;《Bioorganic & Medicinal Chemistry Letters》;20160331;第26卷(第5期);第1391-1396页 * |
| 绿豆芽生产过程中微生物的生长、分布及消毒剂处理效果评价;罗欣 等;《食品与机械》;20170731;第33卷(第7期);第51页摘要 * |
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