CN112425609A - Metarhizium anisopliae preparation for promoting growth of banana plants and preventing and treating banana wilt - Google Patents
Metarhizium anisopliae preparation for promoting growth of banana plants and preventing and treating banana wilt Download PDFInfo
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- CN112425609A CN112425609A CN202011505011.7A CN202011505011A CN112425609A CN 112425609 A CN112425609 A CN 112425609A CN 202011505011 A CN202011505011 A CN 202011505011A CN 112425609 A CN112425609 A CN 112425609A
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- Prior art keywords
- banana
- metarhizium anisopliae
- plants
- medicament
- anisopliae
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M17/00—Apparatus for the destruction of vermin in soil or in foodstuffs
- A01M17/002—Injection of toxic gases or fluids into the soil
Abstract
The invention belongs to the technical field of microorganisms, and particularly relates to a metarhizium anisopliae microbial inoculum for promoting banana plant growth and preventing banana wilt. The invention can obviously promote the growth of banana plants; has good prevention effect on banana vascular wilt.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and particularly relates to a metarhizium anisopliae preparation for promoting banana plant growth and preventing banana wilt.
Background
Bananas (MusaAAA Group) are known as sugarcane, Japanese banana, also known as Chinese dwarf banana, Japanese banana, and pink banana, and are perennial plants of the genus Musa of the family Musaceae. The banana primordial tropical asian region is widely distributed in tropical and subtropical regions between 30 degrees north and south latitude, and at present, more than one hundred countries and regions are used for cultivating bananas (the banana and the health care function thereof, zhuyean, Chinese food and nutrition, 2006, 3 rd stage, 51 st section, 1 st to 4 th line on the left column of page 51, and published 2006, 12 th and 31 th).
The banana plants are large-scale operation, are sent out from the rhizome and form a false stem with the height of 3-6 meters from the lower part of the leaf sheath; the leaves are round to oval, some of the leaves are 3-3.5 meters long, and 10-20 of the leaves cluster with stem tops; the cymbidium-shaped inflorescence sags and is pulled out from the top end of the false rod, most flowers are in light yellow; the fruit is curvy, and the fruit is 20-20 clusters, about 50-150. The plants die after fruiting, and the sucking roots grown from the rhizome continue to reproduce, and each plant can live for many years.
The banana fruit is rich in calcium, phosphorus and iron elements, carotene, thiamine, riboflavin, hydrochloric acid and vitamin C, and contains a small amount of magnesium, sulfur and copper elements, vitamin D, E and the like. The banana fruit contains a large amount of sugar which is easily absorbed by human bodies and also contains a large amount of polyphenol oxidase. The banana is sweet and cold in nature, can clear heat, moisten intestines, moisten lung and detoxify, and has the effects of treating melancholia, preventing gastric ulcer, reducing blood pressure, preventing and treating stroke, relaxing bowel, treating batch rhagadia and the like. In addition, bananas also have the effects of preventing and treating arthritis and ventilation, treating skin inflammation and herpes, relieving skin itch, treating toothache, improving athlete skills, and the like ("bananas and health care functions thereof", zhuyean, chinese food and nutrition, No. 3 in 2006, No. 51 right column, No. 5, No. 1 line to No. 52 right column, No. 4, No. 1 line, published 2006, 12 and 31 days).
However, at present, banana plants are short and restrict the industrial production of bananas.
Disclosure of Invention
In view of the above, the present invention aims to provide a metarhizium anisopliae preparation for promoting the growth of banana plants.
In order to achieve the purpose, the technical scheme of the invention is as follows:
application of Metarrhizium anisopliae in preparation of medicament for promoting banana plant growth.
Further, the metarhizium anisopliae comprises metarhizium anisopliae.
Further, the metarhizium anisopliae comprises metarhizium anisopliae CQMa 421.
Further, the medicament is an oil suspending agent, and the content of the metarhizium anisopliae in the medicament is 20-100 hundred million spores/ml.
The other object of the present invention is to provide a method for promoting the growth of banana plants by root irrigation or soil treatment of banana plants with an agent comprising metarhizium anisopliae.
Furthermore, the content of the metarhizium anisopliae in the medicament is 20-100 hundred million spores/ml.
The invention also aims to protect the application of the metarhizium anisopliae in preparing medicaments for preventing and treating banana vascular wilt (also known as banana yellow leaf disease and banana panama disease).
Further, the metarhizium anisopliae comprises metarhizium anisopliae.
Further, the metarhizium anisopliae comprises metarhizium anisopliae CQMa 421.
Further, the medicament is an oil suspending agent, and the content of the metarhizium anisopliae in the medicament is 20-100 hundred million spores/mL.
The invention has the beneficial effects that:
the invention can obviously promote the growth of banana plants.
The invention has good prevention effect on the banana yellow leaf disease.
The invention has the advantages of safety, high efficiency, no drug residue, no toxic or side effect and the like.
The method is simple and is beneficial to realizing industrial production.
Detailed Description
The examples are provided for better illustration of the present invention, but the present invention is not limited to the examples. Therefore, those skilled in the art should make insubstantial modifications and adaptations to the embodiments of the present invention in light of the above teachings and remain within the scope of the invention.
The following metarhizium anisopliae CQMa421 oil suspending agent is purchased from Chongqing Jiexin bioengineering Co., Ltd, and the spore content is 80 hundred million spores/mL.
Example 1
Growth promoting effect of green muscardine tortoise CQMa421 oil suspending agent on indoor banana potting
Banana variety: brazil banana (available from Guangdong Yitong agricultural development Co., Ltd.);
and (3) test treatment: transplanting the banana seedlings into a pot, after the banana seedlings survive, respectively irrigating roots with 200-time diluent, 400-time diluent and 800-time diluent of metarhizium anisopliae CQMa421 oil suspending agent, and performing root irrigation treatment by using clear water as a control, wherein each pot of plants is treated by 500ml, each pot is treated by 30 pots, and the steps are repeated for 3 times; after the test treatment, the height of the plants is measured in 40 days, the plants are pulled out and cleaned, the dry weights of the overground parts and the roots of the banana plants are respectively weighed after the plants are de-enzymed and dried in an oven, the average values are calculated, statistical analysis data are adopted by SPSS and Excel, and the results are shown in Table 1.
TABLE 1 Metarhizium anisopliae CQMa421 effect on growth promotion of banana
Remarking: the difference in lower case letters indicates a significant difference.
As can be seen from Table 1, compared with the control group, the plant height, the above-ground dry weight and the root dry weight of the banana plants treated by the 200-fold and 800-fold diluted liquid medicine of the metarhizium anisopliae CQMa421 oil suspending agent are obviously improved. Therefore, the root irrigation treatment of the banana plants by using the metarhizium anisopliae CQMa421 oil suspending agent can remarkably promote the growth of the plants and increase the dry matter accumulation of the plants.
Example 2
Verification of effect of metarhizium anisopliae CQMa421 on indoor plate test of banana fusarium wilt
Experimental methods
Fusarium oxysporum f.p. cubense, FOC, a gibbsiella speciality, was provided by life academy of sciences of the university of chongqing.
Transferring the banana vascular wilt disease bacteria cake (diameter 9mm) to be tested to the center of a plate by adopting a plate confronting culture method, inoculating CQMa421 bacteria at 2cm positions around the plate, culturing in a biochemical incubator at 27 ℃, repeating each treatment for 3 times, and taking the average value as a detection result;
when the control group hyphae grow over the plate, the colony diameter and the diameter of the bacteriostatic zone are measured by a cross method, the average value is calculated, the inhibition rate of the tomato anthracnose is calculated according to the following formula, and the result is shown in table 2.
TABLE 2 indoor inhibitory Activity against Banana wilt bacteria
| Test strains | Inhibition ratio (%) |
| Fusarium oxysporum of Guba specialization type | 68.5 |
As can be seen from Table 2, Metarrhizium anisopliae CQMa421 has significant inhibitory effect on the growth of hyphae of banana vascular wilt, and the hyphae growth inhibition rate is 68.5%.
Example 3
Metarhizium anisopliae CQMa421 oil suspending agent for indoor potted planting control of banana wilt
Pathogen: fusarium oxysporum f.p. cubense, FOC, a specialized strain of Cuba Fusarium oxysporum, was provided by the Life sciences college of Chongqing university
Banana variety: brazil banana (available from Guangdong Yitong agricultural development Co., Ltd.);
preparation of pathogen: inoculating Fusarium oxysporum on potato agarose (PDA) culture medium, culturing at 25 deg.C for 7 days, punching 3 fungus cakes on the edges of the colony with a puncher, inoculating the fungus cakes into 100ml potato glucose liquid culture solution, shake culturing at 25 deg.C and 180r/min for 5 days, filtering with 4 layers of sterile mirror wiping paper, adjusting spore concentration to 1 × 10 with a blood count plate6Spores/ml for use.
And (3) test treatment: soaking roots of banana seedlings in 200-time diluted liquid, 400-time diluted liquid, 800-time diluted liquid and clear water of metarhizium anisopliae CQMa421 oil suspending agent respectively before transplanting the banana seedlings into a pot, transplanting the banana seedlings into the pot after soaking for 2h, respectively irrigating the roots of the banana seedlings with liquid medicine corresponding to the root-soaking concentration after the banana seedlings survive, repeating the steps for 3 times for treating 30 bananas, irrigating the roots of the banana seedlings for 500ml each pot plant, irrigating the roots for 1 week for 1 time, irrigating the roots for 3 times totally, inoculating pathogens after treating the roots for 1 week for 1 time by irrigating the roots, wherein the pathogen concentration is 1 multiplied by 106Culturing spores/ml in a pot at the temperature of 25 +/-5 ℃, performing conventional management, observing the growth and blight occurrence conditions of bananas, inoculating for about 35 days, observing the stem and leaf yellowing conditions of overground parts, measuring the height of a plant, counting the disease occurrence conditions, recording the disease level according to a grading standard, and calculating the disease incidence, disease index and prevention effect;
grading standard: grade 0-plants without withered yellow symptoms; level 1-slight withered yellow symptom appears on the lower leaves of the plant, tender leaves are intact, a small part of roots are slightly browned, and the stems are water-stain-shaped browned; level 2-obvious withered and yellow symptoms appear on the lower leaves of the plants, but tender leaves are intact, brown stains appear on the root systems, and water stain-like brown stains appear on the stems and pseudostems; grade 3-withered and yellow symptoms appear on the whole plant, the root system is browned and rotted, the stem part and the pseudostem part are browned and connected, and a few petioles are brownish red; grade 4-the plants show withered and dead symptoms, and the root systems are seriously browned and rotten.
The calculation method comprises the following steps: calculating the morbidity, disease index and prevention effect according to the survey data;
incidence (%) ═ number of diseased plants/total number of investigated plants × 100;
disease index: and (4) calculating according to the formula (1), and reserving 1 bit after the decimal point.
In the formula:
x is disease index;
Ni-number of diseased leaves in each stage;
i-relative step number;
n-survey total leaf number.
In the formula:
p-control effect, in percent (%);
CK-placebo disease index;
PT-agent treatment disease index.
The prevention and treatment effect is as follows: and (4) calculating according to the formula (2), and reserving 1 bit after the decimal point.
Statistical analysis: the data were statistically analyzed using SPSS and Excel, and the results are shown in table 3.
TABLE 3 determination of control effect in indoor pot culture test
| Treatment of | Incidence (%) | Index of disease condition | Control effect (%) | Plant height (cm) |
| 200 times of diluent | 43.3b | 19.2b | 56.6a | 61.6a |
| 400 times of diluent | 50.0b | 20.8b | 52.9a | 59.8a |
| 800 times of diluent | 53.3b | 21.7b | 51.0a | 58.9a |
| Clean water | 90.0a | 41.2a | - | 43.0b |
Remarking: the difference in lower case letters indicates a significant difference.
As can be seen from Table 3, compared with the clear water group, the heights of the banana plants treated by the metarhizium anisopliae oil suspending agent with 200 times of diluent, 400 times of diluent and 800 times of diluent are obviously improved. Therefore, the metarhizium anisopliae can remarkably promote the growth of banana plants.
Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (10)
1. Application of Metarrhizium anisopliae in preparation of medicament for promoting banana plant growth.
2. The use of claim 1, wherein the metarhizium anisopliae comprises metarhizium anisopliae.
3. The use of claim 2, wherein the metarhizium anisopliae comprises metarhizium anisopliae CQMa 421.
4. The use according to any one of claims 1 to 3, wherein the medicament is an oil suspension and the content of Metarhizium anisopliae in the medicament is 20 to 100 hundred million spores/ml.
5. A method for promoting the growth of banana plants is characterized in that the banana plants are subjected to root irrigation treatment or soil treatment by adopting a medicament comprising Metarrhizium anisopliae.
6. The method of claim 5, wherein the agent comprises 20 to 100 billion spores/ml of Metarhizium anisopliae.
7. Application of Metarrhizium anisopliae in preparing medicament for preventing and treating banana vascular wilt is provided.
8. The use of claim 7, wherein the metarhizium anisopliae comprises metarhizium anisopliae.
9. The use of claim 8, wherein the metarhizium anisopliae comprises metarhizium anisopliae CQMa 421.
10. The use according to any one of claims 7 to 9, wherein the medicament is an oil suspension and the amount of metarhizium anisopliae in the medicament is 20 to 100 billion spores/mL.
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105385643A (en) * | 2015-12-27 | 2016-03-09 | 中国热带农业科学院南亚热带作物研究所 | Compound microbial agent for preventing and treating banana wilt disease and prevention and treatment method |
| CN105732216A (en) * | 2016-02-15 | 2016-07-06 | 海南大学 | Compound inoculant used for producing liquid organic fertilizer with fish meal wastewater and application |
| CN107027811A (en) * | 2016-11-02 | 2017-08-11 | 广西农战士高工效农业技术有限公司 | A kind of composition pesticide and its medicament-fertilizer mixture containing the composition pesticide |
| CN107312734A (en) * | 2017-08-21 | 2017-11-03 | 广东省农业科学院农业资源与环境研究所 | Banana blight Antagonistic Fungi and biological organic fertilizer and its preparation method and application |
| CN109964939A (en) * | 2017-12-27 | 2019-07-05 | 营口富里实业有限公司 | A kind of botanical pesticide for preventing and treating wilt disease |
| CN110402967A (en) * | 2019-08-19 | 2019-11-05 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae bacterial strain in the medicament of preparation prevention and treatment gray mold |
| US20190338317A1 (en) * | 2015-11-18 | 2019-11-07 | Provivi, Inc. | Microorganisms for the production of insect pheromones and related compounds |
| CN110432288A (en) * | 2019-08-19 | 2019-11-12 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae CQMa421 in the medicament of preparation prevention and treatment bean sprout rot disease |
| CN110447661A (en) * | 2019-08-19 | 2019-11-15 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae in the medicament of the preparation prevention and treatment late blight of potato |
| CN110521736A (en) * | 2019-07-22 | 2019-12-03 | 华中农业大学 | Application of the Metarhizium anisopliae on prevention and treatment sclerotinia sclerotiorum |
-
2020
- 2020-12-18 CN CN202011505011.7A patent/CN112425609A/en active Pending
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20190338317A1 (en) * | 2015-11-18 | 2019-11-07 | Provivi, Inc. | Microorganisms for the production of insect pheromones and related compounds |
| CN105385643A (en) * | 2015-12-27 | 2016-03-09 | 中国热带农业科学院南亚热带作物研究所 | Compound microbial agent for preventing and treating banana wilt disease and prevention and treatment method |
| CN105732216A (en) * | 2016-02-15 | 2016-07-06 | 海南大学 | Compound inoculant used for producing liquid organic fertilizer with fish meal wastewater and application |
| CN107027811A (en) * | 2016-11-02 | 2017-08-11 | 广西农战士高工效农业技术有限公司 | A kind of composition pesticide and its medicament-fertilizer mixture containing the composition pesticide |
| CN107312734A (en) * | 2017-08-21 | 2017-11-03 | 广东省农业科学院农业资源与环境研究所 | Banana blight Antagonistic Fungi and biological organic fertilizer and its preparation method and application |
| CN109964939A (en) * | 2017-12-27 | 2019-07-05 | 营口富里实业有限公司 | A kind of botanical pesticide for preventing and treating wilt disease |
| CN110521736A (en) * | 2019-07-22 | 2019-12-03 | 华中农业大学 | Application of the Metarhizium anisopliae on prevention and treatment sclerotinia sclerotiorum |
| CN110402967A (en) * | 2019-08-19 | 2019-11-05 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae bacterial strain in the medicament of preparation prevention and treatment gray mold |
| CN110432288A (en) * | 2019-08-19 | 2019-11-12 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae CQMa421 in the medicament of preparation prevention and treatment bean sprout rot disease |
| CN110447661A (en) * | 2019-08-19 | 2019-11-15 | 重庆谷百奥生物研究院有限公司 | Application of the Metarhizium anisopliae in the medicament of the preparation prevention and treatment late blight of potato |
Non-Patent Citations (3)
| Title |
|---|
| GARCÍA JULIA ELENA等: "Metarhizium anisopliae (Metschnikoff) Sorokin Promotes Growth and Has Endophytic Activity in Tomato Plants", 《ADVANCES IN BIOLOGICAL RESEARCH》 * |
| I.L. BISUTTI等: "Influence of antagonistic micro-organisms on the growth of strawberry plants in the presence of Verticillium dahliae and Phytophthora cactorum", 《SHORT CONTRIBUTIONS》 * |
| 齐永霞等: "金龟子绿僵菌对棉花枯萎病菌的拮抗作用研究", 《棉花学报》 * |
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Application publication date: 20210302 |