CN114469812A

CN114469812A - Fermented turmeric gel and lavender flower water compound essence and preparation method thereof

Info

Publication number: CN114469812A
Application number: CN202210153016.0A
Authority: CN
Inventors: 魏娟; 陈旻; 庞颖; 潘莎
Original assignee: Hangzhou Formulator Technology Co ltd
Current assignee: Hangzhou Formulator Technology Co ltd
Priority date: 2022-02-18
Filing date: 2022-02-18
Publication date: 2022-05-13

Abstract

The invention discloses a fermented turmeric gel and lavender flower water compound essence and a preparation method thereof, wherein the essence comprises the following components in percentage by mass: 1-20% of a compound product, 0-7% of an emulsifier, 0-10% of an emollient, 0-3% of a thickener, 3-10% of a humectant, 0-3% of a skin conditioner and 50-90% of water, wherein the compound product is obtained by mixing a fermentation type turmeric gel and lavender flower water according to a ratio of 2: 1-6 and then carrying out ultrasonic treatment, and the fermentation type turmeric gel is obtained by fermenting turmeric powder by using lactic acid bacteria and carrying out ethanol extraction, reduced pressure concentration and freeze drying; when the aqua essence is prepared, water, humectant and thickener are mixed and heated in proportion, and are homogenized and stirred uniformly; and (3) adding the skin conditioner after the primary cooling, uniformly stirring, adding the compound product after the secondary cooling, and uniformly stirring to obtain the aqua essence. The invention has the advantages that the effective components in the fermented turmeric gel and the lavender flower water cooperate with each other to resist bacteria and oxidation, and the acne can be quickly and long-effectively removed and inhibited.

Description

Fermented turmeric gel and lavender flower water compound essence and preparation method thereof

Technical Field

The invention belongs to the technical field of essence, and particularly relates to a fermentation type turmeric gel and lavender flower water compound essence and a preparation method thereof.

Background

Curcumin (curculin) is a diketone plant polyphenol substance extracted from Chinese medicinal material Curcuma rhizome, and has a chemical formula of C and a content of about 3-6% in Curcuma rhizome₂₁H₂₀O₆Orange yellow crystalline powder, insoluble in water. Curcumin is found to have antibacterial, anti-tumor, anti-inflammatory, antioxidant and myocardial injury effects in recent research, and can be used for clinical treatment of tumor, osteoarthritis, spinal cord injury, etc. The poor water solubility of the compound causes low bioavailability, and the application of the compound is greatly limited.

Lavender flower water is byproduct of lavender essential oil extraction process, is distilled water generated by steam distillation of Lavender, and has low concentration of effective component mainly including eucalyptol (C)₁₀H₁₈O), limonene oxide, 3-octyl acetate and the like, but also has bactericidal and antioxidant activities and is widely applied to skin care products.

Due to the reasons of high modern life pressure, unhealthy living habits, poor air quality and the like, the number of people with skin acne problems is increasing year by year. The pathogenesis of acne mainly comprises androgen secretion abnormality, mass propagation of propionibacterium acnes, inflammatory damage, immune disorder, follicular sebaceous gland duct keratosis and the like. The moderate and severe acne needs to be treated by adopting medical means and simultaneously carrying out oral administration and external medicine treatment. For mild and moderate acne, acne-removing skin care products can be selected to solve the acne trouble. In addition, when skin cells of the acne muscle population are damaged by inflammation, the organism can generate various free radicals, the reactivity is strong, the organism can rapidly react with various biomolecules in living cells to cause DNA, cell membranes and other signal molecules to be damaged, the aging and the darkness of the skin are reflected, and the skin care product using the antioxidant component can immediately resist the damage of the free radicals and repair the skin barrier.

Most of acne-removing essence in the market is only concentrated in solving the problem of skin inflammation or rapidly changing skin by using stripping components, can not continuously and effectively realize the acne-removing and acne-inhibiting effects, and easily causes the skin barrier to be damaged.

Disclosure of Invention

The invention aims to solve the problems in the background art and provides a compound essence of fermented turmeric gel and lavender flower water and a preparation method thereof, which can enable active ingredients in the fermented turmeric gel and the lavender flower water to cooperate with antibacterial and antioxidant effects, realize quick and long-acting acne removing and acne inhibiting effects under the condition of no acid stripping, quickly repair skin and maintain stable skin.

In order to achieve the purpose, the invention provides a fermentation type turmeric gel and lavender flower water compound essence which comprises the following components in percentage by mass: the skin conditioner is characterized by comprising the following components, by weight, 1-20% of a compound product, 0-7% of an emulsifier, 0-10% of an emollient, 0-3% of a thickener, 3-10% of a humectant, 0-3% of a skin conditioner and 50-90% of water, wherein the compound product is obtained by mixing a fermentation type turmeric gel and lavender flower water according to a ratio of 2: 1-6 and then carrying out ultrasonic treatment, and the fermentation type turmeric gel is obtained by fermenting turmeric powder with lactic acid bacteria and carrying out ethanol extraction, reduced pressure concentration and freeze drying.

Preferably, the fermentation type turmeric gel is prepared by the following preparation method:

s01, culturing and proliferating lactic acid bacteria in an MRS culture medium to obtain a culture solution;

s02, inoculating a culture medium containing turmeric powder to the culture solution for fermentation, maintaining for 2-3 hours after the pH of fermentation liquor is reduced to 4-4.3 to complete fermentation, adjusting the pH of the fermentation liquor to 6.5-7.0 after the fermentation is finished, and centrifuging and filtering to obtain a supernatant;

s03, carrying out primary reduced pressure concentration on the supernatant, extracting with ethanol, carrying out secondary reduced pressure concentration, heating to evaporate part of ethanol, adding a drying auxiliary agent, and carrying out freeze drying to obtain a fermentation type turmeric gel;

wherein the lactobacillus is selected from Lactobacillus plantarum with the strain number of CGMCC 1.12934.

Preferably, the drying auxiliary agent is starch, and the MRS culture medium contains 2% of glucose, 2% of yeast powder, 2% of peptone, 0.5% of sodium acetate, 0.2% of sodium citrate and 0.1% of CaCl₂And 0.1% of Tween 80, wherein the culture medium contains 3-5% of turmeric powder, 1% of glucose, 1% of peptone, 0.5% of sodium acetate, 0.2% of sodium citrate and 0.1% of CaCl₂0.1% tween 80.

Preferably, the moisturizer comprises at least one of polyethylene glycol-32, glyceryl polyether-26, propylene glycol, allantoin, 1, 2-hexanediol, niacinamide, 1, 2-pentanediol, glycerin, propylene glycol, butylene glycol, arginine, phytosphingosine, panthenyl ethyl ether.

Preferably, the thickener includes at least one of sodium acryloyldimethyl taurate, xanthan gum, stearic acid, diphenyldimethicone/vinyldiphenyldimethicone/silsesquioxane crosspolymer, cetyl alcohol.

Preferably, the skin conditioning agent comprises at least one of p-hydroxyacetophenone, caprylhydroxamic acid, 1, 2-hexanediol, butylene glycol, and ethylhexylglycerin.

Preferably, the ultrasonic treatment is carried out at an ultrasonic power of 200W and at 25 ℃.

Preferably, the emulsifier comprises at least one of glyceryl stearate, PEG-100 stearate, cetearyl alcohol, hydrogenated lecithin, isocetyl stearate, PEG-150 pentaerythritol tetrastearate, ceteth-30, and the emollient comprises at least one of polydimethylsiloxane, hydrogenated ethylhexyl olivate, isononyl isononanoate, cyclopentadimethylsiloxane, cyclohexasiloxane, PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, caprylic/capric triglyceride, cocoa butter, cetyl ethylhexanoate, tri (ethylhexanoate), petrolatum.

The invention also provides a preparation method of the essence, wherein the essence is water essence prepared by the following steps: mixing water, a humectant and a thickening agent in proportion, heating to 75-85 ℃, and homogenizing and stirring until the material is uniform; cooling to 45-50 ℃, adding a skin conditioner, stirring uniformly, continuously cooling to below 45 ℃, adding a compound product, and stirring until the material body is uniform to obtain the aqua essence.

The invention also provides a preparation method of the essence, wherein the essence is emulsified essence, and the emulsified essence is prepared by the following steps: mixing water, a humectant and a thickening agent in proportion, heating to 75-85 ℃, and homogenizing and stirring until the material is uniform to obtain a phase A; mixing and heating the emulsifier and the emollient to 75-85 ℃ in proportion, and uniformly stirring to obtain a phase B; adding the phase B into the phase A, homogenizing and stirring until emulsification is uniform, cooling to 45-50 ℃, adding a skin conditioner, stirring uniformly, continuously cooling to below 45 ℃, adding a compound product, and stirring uniformly to obtain the emulsified body essence.

The invention has the beneficial effects that: according to the invention, the fermentation type turmeric gel and the lavender flower water are compounded according to a certain proportion, and ultrasonic treatment is carried out, so that multiple effective components are cooperated to realize antibiosis and antioxidation, the acne removing and suppressing effects with a long-acting effect are realized under the condition of not using acid stripping, the skin is repaired quickly, and the skin is maintained to be stable; the curcumin in the turmeric powder is enriched by using a fermentation technology, the water solubility of the curcumin is enhanced, the bioavailability is improved, and a fermentation type turmeric gel product with good water solubility, high curcumin content and no coloration is obtained, and the product has inflammation inhibition and broad-spectrum antibacterial effects.

The features and advantages of the present invention will be described in detail by embodiments in conjunction with the accompanying drawings.

Drawings

Fig. 1 is a flow chart of a manufacturing process of a fermented turmeric gel according to an embodiment.

FIG. 2 is a graph comparing DPPH clearance for an example fermented turmeric gel, lavender floral water and a formulated product.

FIG. 3 is a comparative illustration of the antimicrobial properties of an example fermented turmeric gel, lavender floral water and a combination product.

Detailed Description

The invention provides a fermentation type turmeric gel and lavender flower water compound essence, which comprises the following components in percentage by mass: 1-20% of a compound product, 0-7% of an emulsifier, 0-10% of an emollient, 0-3% of a thickener, 3-10% of a humectant, 0-3% of a skin conditioner and 50-90% of water, wherein the compound product is obtained by mixing a fermentation type turmeric gel and lavender flower water according to a ratio of 2: 1-6 and then carrying out ultrasonic treatment, the fermentation type turmeric gel is obtained by fermenting turmeric powder by using lactic acid bacteria and carrying out ethanol extraction, reduced pressure concentration and freeze drying, wherein the ultrasonic treatment is carried out at an ultrasonic power of 200W and at a temperature of 25 ℃, and the ultrasonic treatment time is 2 hours.

The humectant comprises at least one of polyethylene glycol-32, glyceryl polyether-26, propylene glycol, allantoin, 1, 2-hexanediol, nicotinamide, 1, 2-pentanediol, glycerol, propylene glycol, butylene glycol, arginine, phytosphingosine, and panthenyl ethyl ether.

The thickener comprises at least one of sodium acryloyl dimethyl taurate, xanthan gum, stearic acid, diphenyl dimethyl siloxane/vinyl diphenyl dimethyl siloxane/silsesquioxane cross-linked polymer and cetyl alcohol.

The skin conditioner comprises at least one of p-hydroxyacetophenone, caprylhydroxamic acid, 1, 2-hexanediol, butylene glycol, and ethylhexylglycerin.

The emulsifier comprises at least one of glyceryl stearate, PEG-100 stearate, cetearyl alcohol, hydrogenated lecithin, isocetyl stearate, PEG-150 pentaerythritol tetrastearate, ceteth-30, and the emollient comprises at least one of dimethicone, hydrogenated ethylhexyl olivate, isononyl isononanoate, cyclopentadimethicone, cyclohexasiloxane, PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, caprylic/capric triglyceride, cocoa butter, cetyl ethylhexanoate, glyceryl tri (ethylhexanoate), petrolatum.

Referring to fig. 1, the fermented turmeric gel is specifically prepared according to the following preparation method:

s01, putting lactobacillus in MRCulturing and proliferating in S culture medium to obtain culture solution, wherein MRS culture medium contains 2% glucose, 2% yeast powder, 2% peptone, 0.5% sodium acetate, 0.2% sodium citrate, and 0.1% CaCl₂0.1 percent of Tween 80, and the culture of the lactic acid bacteria is carried out under the condition of keeping the temperature at 37 ℃ for 24 hours;

s02, inoculating a culture medium containing turmeric powder to the culture solution, placing the culture solution on a shaking table at 37 ℃ and 50rpm for fermentation, maintaining the pH of the fermentation solution for 2-3 hours after the pH of the fermentation solution is reduced to 4-4.3 to complete the fermentation, adjusting the pH of the fermentation solution to 6.5-7.0 by using 10% NaOH after the fermentation is finished, centrifuging and filtering to obtain a supernatant, wherein the culture medium contains 3-5% of turmeric powder, 1% of glucose, 1% of peptone, 0.5% of sodium acetate, 0.2% of sodium citrate and 0.1% of CaCl₂0.1% tween 80;

s03, carrying out primary reduced pressure concentration on the supernatant, extracting with 2-3 times of ethanol, carrying out secondary reduced pressure concentration, heating to evaporate most of ethanol, adding starch, and carrying out freeze drying to obtain a fermentation type turmeric gel;

the Lactobacillus is selected from Lactobacillus plantarum with the strain number of CGMCC1.12934, the fermentation type turmeric gel is light yellow transparent gel and has good water solubility, high molecules such as plant fibers and proteins in the turmeric are converted into organic acids after the Lactobacillus is fermented, the organic acids increase the solubility of curcumin on one hand, and on the other hand, curcumin precursors existing in turmeric powder effectively synthesize the curcumin so as to improve the content of the curcumin in the turmeric gel, the curcumin precursors comprise coumaric acid and derivatives thereof, ferulic acid and derivatives thereof, and the curcumin mentioned in the application is curcumin (C)₂₁H₂₀O₆) Demethoxycurcumin (C)₂₀H₁₈O₅) And bisdemethoxycurcumin (C)₁₉H₁₆O₄) Is a general term for (1).

Compared with other fermentation strains, the fermentation strain with the strain number has excellent morphological state of the lactic acid bacteria during culture, and the curcumin content in the fermentation liquor after fermentation by the fermentation strain with the strain number is obtained through comparison by establishing an absorbance standard curve of curcumin ethanol solutions with different concentrations at 405nm through an enzyme linked immunosorbent assay instrument and fitting to determine the curcumin content in the fermentation liquor of each strain.

Antioxidant analysis on the compounded product: dividing a plurality of identical color tubes with plugs into four groups, adding 2ml of sample into the four groups of tubes, respectively, wherein the four samples are respectively fermentation type turmeric gel, lavender flower water, compound product (subjected to ultrasonic treatment) and compound product (not subjected to ultrasonic treatment), and adding 2ml of 1 × 10 into the four groups of tubes respectively^-4And (3) uniformly mixing the DPPH ethanol solution in mol/L, storing the mixture at room temperature in a dark place for 30min, and measuring the absorbance value of the mixture at the wavelength of 517nm by using an ultraviolet spectrophotometer, wherein the specific detection result is shown in figure 2. DPPH clearance was calculated as follows:

in the formula, A₀The absorbance value of 2ml of DPPH solution added with 2ml of ethanol solution; a. the₁Adding 2ml of ethanol solution into 2ml of sample solution to obtain a light absorption value; a. the₂Absorbance values for 2ml sample solution plus 2ml dpph solution.

As can be seen from fig. 2, the DPPH clearance of the compounded product (subjected to ultrasonic treatment) is far higher than the sum of DPPH clearance of single components such as the fermented turmeric gel and the madecassoside, and the DPPH clearance of the compounded product (subjected to ultrasonic treatment) is higher than the DPPH clearance of the compounded product (not subjected to ultrasonic treatment), so that the effective components in the fermented turmeric gel and the lavender flower water realize synergistic antioxidation through ultrasonic treatment, and the overall inoxidizability of the essence is improved.

And (3) antibacterial property analysis of the compound product: and (3) measuring the antibacterial effect of the fermentation type turmeric gel sample by using a filter paper sheet diffusion method. The specific determination method is as follows: a plurality of round sterile filter paper sheets with the diameter of 6mm are equally divided into four groups, the two groups of sterile filter paper sheets are respectively put into equal amounts of fermentation type turmeric gel, lavender flower water, a compound product (subjected to ultrasonic treatment) and a compound product (not subjected to ultrasonic treatment) sample solution to be soaked for 30min, 0.1ml of different bacterial suspensions (staphylococcus aureus, escherichia coli and bacillus subtilis) are dropwise added on a solid culture medium to be uniformly coated, the filter paper sheets clamped and soaked by sterile clamps are pasted on the culture medium after being dried slightly, tweezers are used for culturing for 12h at 37 ℃, observation results are obtained after 24h and 36h, the three bacteria are obviously inhibited in 12h, the inhibition effect is weakened in 24h, the inhibition effect is invalid later, the diameter of an inhibition zone is measured for 12h, and the specific measurement result is shown in figure 3.

As can be seen from fig. 3, the bacteriostatic diameter of the compound product (after ultrasonic treatment) is significantly larger than that of the sample of the fermented turmeric gel, lavender flower water and the compound product (without ultrasonic treatment), the compound product can effectively prolong the bacteriostatic time, and the ultrasonic treatment has a certain promotion effect on the compound product in prolonging the bacteriostatic time.

The invention is further illustrated by the following specific examples, which are intended to be illustrative only and not limiting:

example 1

The fermented turmeric gel and lavender flower water compound essence is aqueous essence, and the aqueous essence consists of the following components in percentage by mass: 10% of compound product, 0.1% of allantoin, 0.6% of p-hydroxyacetophenone, 0.3% of xanthan gum, 3% of 1, 2-hexanediol, 3% of glycerol and 83% of water.

The aqueous essence is prepared by the following method: mixing water, allantoin, glycerol and xanthan gum in proportion, heating to 80 ℃, homogenizing and stirring until the mixture is completely dissolved to obtain a phase A; and (3) when the phase A is cooled to 50 ℃, adding the pre-dissolved p-hydroxyacetophenone and the 1, 2-hexanediol, uniformly stirring, continuously cooling to below 40 ℃, adding the compound product, stirring until the system is completely transparent, and uniformly mixing the materials to obtain the aqua essence.

Example 2

The fermented turmeric gel and lavender flower water compound essence is emulsified body essence, and the emulsified body essence comprises the following components in percentage by mass: 10% of compound product, 0.1% of allantoin, 0.8% of p-hydroxyacetophenone, 0.5% of xanthan gum, 5% of 1, 2-hexanediol, 3% of glycerol, 1% of glycerol isostearate, 2% of polydimethylsiloxane, 2% of caprylic/capric triglyceride and 75.6% of water.

The emulsion essence is prepared by the following method: mixing water, glycerol, allantoin and xanthan gum in proportion, heating to 80 deg.C, homogenizing and stirring to obtain phase A; mixing and heating the isostearic acid ester, the polydimethylsiloxane and the caprylic/capric triglyceride according to a proportion to 80 ℃, and stirring for 30min to ensure that the material body is uniform to obtain a phase B; adding phase B into phase A, homogenizing and stirring for 30min until emulsification is uniform, cooling to 50 deg.C, adding pre-dissolved p-hydroxyacetophenone and 1, 2-hexanediol, stirring, continuously cooling to below 40 deg.C, adding the compound product, and stirring until the material is uniform to obtain emulsified essence.

The above embodiments are illustrative of the present invention, and are not intended to limit the present invention, and any simple modifications of the present invention are within the scope of the present invention.

Claims

1. A fermentation type turmeric gel and lavender flower water compound essence is characterized in that: the essence consists of the following components in percentage by mass: the skin conditioner is characterized by comprising the following components, by weight, 1-20% of a compound product, 0-7% of an emulsifier, 0-10% of an emollient, 0-3% of a thickener, 3-10% of a humectant, 0-3% of a skin conditioner and 50-90% of water, wherein the compound product is obtained by mixing a fermentation type turmeric gel and lavender flower water according to a ratio of 2: 1-6 and then carrying out ultrasonic treatment, and the fermentation type turmeric gel is obtained by fermenting turmeric powder with lactic acid bacteria and carrying out ethanol extraction, reduced pressure concentration and freeze drying.

2. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the fermentation type turmeric gel is prepared by the following preparation method:

3. The essence of claim 2, wherein: the drying auxiliary agent is starch, and the MRS culture medium contains 2% of glucose, 2% of yeast powder, 2% of peptone, 0.5% of sodium acetate, 0.2% of sodium citrate and 0.1% of CaCl₂And 0.1% of Tween 80, wherein the culture medium contains 3-5% of turmeric powder, 1% of glucose, 1% of peptone, 0.5% of sodium acetate, 0.2% of sodium citrate and 0.1% of CaCl₂0.1% tween 80.

4. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the humectant comprises at least one of polyethylene glycol-32, glyceryl polyether-26, propylene glycol, allantoin, 1, 2-hexanediol, nicotinamide, 1, 2-pentanediol, glycerol, propylene glycol, butylene glycol, arginine, phytosphingosine, and panthenyl ethyl ether.

5. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the thickening agent comprises at least one of sodium acryloyl dimethyl taurate, xanthan gum, stearic acid, diphenyl polydimethylsiloxane/vinyl diphenyl polydimethylsiloxane/silsesquioxane cross-linked polymer and cetyl alcohol.

6. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the skin conditioner comprises at least one of p-hydroxyacetophenone, caprylhydroxamic acid, 1, 2-hexanediol, butanediol and ethylhexyl glycerin.

7. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the ultrasonic treatment is carried out under the conditions of ultrasonic power of 200W and 25 ℃.

8. The fermented turmeric gel and lavender flower water compounded essence of claim 1, wherein: the emulsifier comprises at least one of glyceryl stearate, PEG-100 stearate, cetearyl alcohol, hydrogenated lecithin, isocetyl stearate, PEG-150 pentaerythritol tetrastearate, ceteth-30, and the emollient comprises at least one of polydimethylsiloxane, hydrogenated ethylhexyl olivate, isononyl isononanoate, cyclopentadimethicone, cyclohexasiloxane, PPG-17/IPDI/DMPA copolymer, PEG/PPG-10/30 copolymer, caprylic/capric triglyceride, cocoa butter, cetyl ethylhexanoate, tri (ethylhexanoate) glyceride, petrolatum.

9. A method for preparing the essence according to any one of claims 1 to 7, wherein: the essence is water essence which is prepared by the following method: mixing water, a humectant and a thickening agent in proportion, heating to 75-85 ℃, and homogenizing and stirring until the material is uniform; cooling to 45-50 ℃, adding a skin conditioner, stirring uniformly, continuously cooling to below 45 ℃, adding a compound product, and stirring until the material body is uniform to obtain the aqua essence.

10. A method for preparing the essence according to any one of claims 1 to 8, characterized in that: the essence is emulsified essence, and the emulsified essence is prepared by the following method: mixing water, a humectant and a thickening agent in proportion, heating to 75-85 ℃, and homogenizing and stirring until the material is uniform to obtain a phase A; mixing and heating the emulsifier and the emollient to 75-85 ℃ in proportion, and uniformly stirring to obtain a phase B; adding the phase B into the phase A, homogenizing and stirring until emulsification is uniform, cooling to 45-50 ℃, adding a skin conditioner, stirring uniformly, continuously cooling to below 45 ℃, adding a compound product, and stirring uniformly to obtain the emulsified body essence.