KR101106690B1 - A composition for anti-sebum and acne-remedy cosmetics - Google Patents

Abstract

The present invention relates to a cosmetic composition for sebum suppression and acne improvement, which comprises at least one extract selected from the group consisting of a counter electrode and a repellent.

The use of the cosmetic composition containing the extract of the present invention or the extract of the present invention suppresses the activity of 5-alpha-reductase and inhibits the production of active male hormone and inhibits the growth of Propionibacterium acnes, Thereby preventing sebum secretion and improving acne. Further, since the composition of the present invention contains a natural herbal extract, it can be safely used because it has no side effects even when it is used for a long time.

Antipode, prescription, sebum, acne, cosmetics, composition

Description

[0001] The present invention relates to a composition for anti-sebum and acne-remedy cosmetics,

The present invention relates to a cosmetic composition for suppressing sebum and improving acne, and more specifically, to a cosmetic composition for sebum suppression and acne improvement, which contains at least one extract selected from the group consisting of antiperspirant and depressed.

Acne is associated with sebum and is caused by excess sebum produced by male hormones. In order to provide fundamental treatment, it is necessary to suppress the excessive production of sebum by inhibiting the activity of male hormone.

Testosterone, one of the male hormones, is converted to the active male hormone dihydrotestosterone (DHT) by 5-alpha-reductase, and abnormal hyperactivity of this hormone is found in sebaceous glands sebocyte, which causes sebum to act as a major cause of overproduction. Therefore, inhibiting the activity of 5-alpha-reductase, which induces excessive production of dihydrotestosterone, can prevent and treat acne fundamentally and effectively.

In addition, when the sebum produced is not discharged out of the pores, primary micro-comedones are formed, and secondary inflammation is caused by the action of the anaerobic skin microorganism, Propionibacterium acnes, which is present in the sebum of the pores. Which causes acne.

Therefore, in order to treat the above-mentioned acne, anti-androgens used to inhibit sebum production, nonsteroidal anti-inflammatory agents used as anti-inflammatory agents, resorcinol, sulfur and benzoylperoxide having antibiotic effect, Erythromycin, macrocycline and the like, and the like. Recently, a treatment method using a vitamin A derivative such as retinoic acid has also been widely used.

However, the above methods have some effects on acne treatment and prevention, but have side effects in terms of skin safety. In other words, the use of hormones may cause adverse effects such as inhibition of growth of the epidermis. Retinoic acid and benzoyl peroxide may cause skin irritation and contact dermatitis. Antibiotics such as tetracycline may cause bacterial and photosensitization Problems are reported.

In order to solve the above problems, the present inventors have intensively studied the activity of 5α-reductase, an enzyme directly involved in the production of active male hormone, which is a fundamental cause of acne, As a result of repeated studies on various herbal medicines in order to find a substance which has excellent antimicrobial action against Propionibacterium acnes which is known as a major cause of acne deterioration and which is safe even after prolonged use, The present invention has been accomplished based on the discovery that the anticoporant extract having action and antimicrobial action and the suppressant known as having immunity and antibacterial action have an excellent therapeutic effect against acne.

Accordingly, it is a main object of the present invention to provide a cosmetic composition for acne skin which contains sebum extract and extract as an active ingredient and exhibits sebum suppression and excellent acne treatment effect.

According to one aspect of the present invention, there is provided a cosmetic composition for the prevention of sebum and for improving acne, which comprises at least one extract selected from the group consisting of a counter electrode and a retractor.

In the cosmetic composition of the present invention, the extract is preferably contained in an amount of 0.001 to 10% by weight, based on the total weight of the cosmetic composition, by dry weight.

According to another aspect of the present invention, there is provided a method for inhibiting sebum and improving acne, comprising the step of adding an extraction solvent having a concentration of 1 to 5 vol. The present invention provides a method for producing a cosmetic composition for use in cosmetics.

According to still another aspect of the present invention, there is provided a method for producing a fermented milk, comprising the step of adding 1 to 5 vol of the extraction solvent to the mixture of the counter electrode and the feed of 0.01 to 1: 1 to 0.01 weight ratio, heating or cooling the mixture, And a method for producing a cosmetic composition for suppressing sebum and improving acne.

In the process for producing a cosmetic composition according to the present invention, it is preferable that the extraction solvent is a mixture of one or more selected from the group consisting of lower alcohols having 1 to 5 carbon atoms, hexane, ethyl acetate and diethyl ether.

In the method for producing a cosmetic composition according to the present invention, the heating is preferably performed at a temperature of 50 to 100 ° C for 1 to 10 hours.

In the method for producing a cosmetic composition according to the present invention, it is preferable that the cold beating is performed at a temperature of 0 to 40 ° C for 1 to 30 days.

Hereinafter, the cosmetic composition for preventing sebum and improving acne according to the present invention will be described in more detail step by step.

The present invention relates to a cosmetic composition which uses sebum suppression effect and acne improvement effect of a herbal medicine or a herbal medicine. The main pole refers to the root of Euphorbia pekinensis Ruprecht which belongs to the Euphorbiaceae , It is known to have components such as euphorbic acid, euphorbone, and euphorin. The pharmacological actions include diarrhea, diuretic action, antimicrobial action, etc., and sterilization and insecticidal effect are recognized for external use.

It is also known that Cretotympana pustulata Fabricus belongs to Cicadidae, and that it is a shell that melts when it becomes an adult of insects. It is wild in many parts of Korea, Japan and China and contains a large amount of chitin, flavonoid, Sterols, proteins, organic acids, phenols, amino acids, and fatty acids. Pharmacological actions include anticonvulsant action, sedative action, analgesic action, antiallergic action, antibacterial action, taking tetanus, chlamydia, and purulent otitis media and taking it out or taking it out.

The extracts of the antipodes and the extracts used in the present invention can be prepared by leaching or transferring from the herbal medicines or by concentrating the whole or part of the leaching solution again or by drying the concentrates again, And the chemical substance itself contained in the herbal medicines and exhibiting the main effect.

Examples of the method for extracting the medicinal herb extract of the present invention include a method of extracting the medicinal herb with a lower alcohol having 1 to 5 carbon atoms, hexane, ethyl acetate or diethyl ether, or a method of extracting hexane, ethyl acetate, A method of extracting a fraction of the water layer is used. In the case of the counter electrode, methanol is most preferably added and extracted by heating, and ethyl acetate is most preferably used in the case of repelling. The extraction method will be described in more detail as follows.

A commercially available counter electrode and a commercially available counter electrode were purchased, and only a genuine product was finely pulverized. An organic solvent such as ethanol or methanol was added to the pulverized product in an amount of 3 to 5 times as much as the dry weight of the pulverized product. To 100 占 폚 for 1 to 10 hours, or extracting the active ingredient by immersing the herbal medicines in the solvent at a temperature of 0 to 40 占 폚 for 1 to 30 days. The two herbal extracts thus extracted may be used in the form of a liquid or dried to be used in a powder state. Preferably, the dried extract is completely dried using a reduced pressure concentrated dryer to be used as a dry powder.

It is preferable that the composition of the present invention contains at least one kind of extract selected from the group consisting of a counter electrode and a repelling agent in an amount of 0.001 to 10% by weight based on the total weight of the composition. This is because the effect is insignificant when it is used in an amount less than 0.001% by weight, and irritation may be caused in the skin when it is used in an amount exceeding 10% by weight.

The composition of the present invention can be formulated into a conventional formulation that can be applied to the skin. For example, the composition of the present invention may be formulated into conventional formulations applicable to the skin, for example, cosmetics such as lotion, cream, lotion, skin lotion, foam, and the like. Each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulations, and the type and amount of these ingredients may be readily selected by those skilled in the art.

Hereinafter, the present invention will be described in more detail with reference to examples and test examples. These embodiments are only for illustrating the present invention, and thus the scope of the present invention is not construed as being limited by these embodiments.

Example 1 Preparation of Dipole Extract

To 100 g of the counter electrode, 150 ml of 70% methanol was added. The mixture was heated by an extractor equipped with a condenser for 2 hours and then filtered twice with Whatmat # 2 filter paper to remove the residue to prepare a counter electrode extract. The extract was concentrated under reduced pressure at 60 ° C using a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain 5.4 g of a dry powder.

Example 2: Preparation of extract

200 g of ethyl acetate was added to 100 g of the supernatant, and the mixture was heated in an extractor equipped with a cooling condenser for about 2 hours and then filtered twice with Whatmat # 2 filter paper to remove debris and make a preliminary extract. The extract was concentrated under reduced pressure at 60 ° C using a distillation apparatus equipped with a cooling condenser and then lyophilized to obtain 6.7 g of a dry powder.

Test Example 1. Test for inhibiting 5-alpha-reductase activity

(0.32 M sucrose, 0.1 mM dithiothreitol, 20 mM sodium acetate) was added to the liver of a male Sprague-Dawley male rats (Sprague-Dawley rats) of 8-10 weeks old and finely pulverized and then pulverized by a pulverizer. The suspension was centrifuged The supernatant was taken and partially purified to prepare a 5-alpha-reductase enzyme reaction solution to be used in this test example.

The radioactive labeled testosterone (3H-testosterone) was used as a substrate for 5-alpha-reductase, and enzyme reaction solution: substrate solution: herbal medicine extract , The dry extract powder of Example 2, or the same amount of the two) were mixed at a weight ratio of 70: 20: 10, respectively, and reacted in a incubator for 2 minutes.

After the reaction, the solvent was removed and the remaining material was dissolved in developing solvent and developed on a thin-layer chromatography plate. The amount of developed testosterone and the amount of dihydrotestosterone were quantified using a densitometer As a result, the conversion rate of testosterone to dihydrotestosterone by 5-alpha-reductase was calculated.

The results were compared with the control group (conversion when the extract was not added) to evaluate the 5-alpha-reductase inhibition rate.

The inhibition rate evaluation method was as follows.

[Equation 1]

5-alpha-reductasease inhibition rate (%) = [(A-B) / A]

A is the conversion of testosterone to dihydrotestosterone at the time of no addition of the extract (control group), and B is the conversion of testosterone to dihydrotestosterone upon addition of the extract.

The results of the test by the method of calculating the inhibition rate are shown in Tables 1 and 2 below.

[Table 1] Conversion rate to dihydrotestosterone

Dry powder concentration (%) 0.1 0.01 0.001 0.0001
Conversion rate% (B)
Example 1 9.2 13.6 48.2 73.1 Example 2 6.8 11.7 45.2 72.3 Example 1 + Example 2 1.9 8.4 42.1 70.6

[Table 2] 5-Alpha-reductase inhibitory rate

Dry powder concentration (%) 0.1 0.01 0.001 0.0001
% Inhibition
Example 1 90.8 86.4 51.8 26.9 Example 2 93.2 88.3 54.8 27.7 Example 1 + Example 2 98.1 91.6 57.9 29.4

As a result, one or more extracts selected from the group consisting of Daphnia and Porphyra were inhibitory to 5-alpha-reductase and the inhibitory rate was more than 50% when the dry powder concentration was over 0.001% Could know.

Test Example 2. Sterilization test for acne bacteria

In order to confirm the effect of preventing and treating acne in each of the dry powders prepared in Examples 1 and 2, the following experiment was conducted.

First, for activation of Propionibacterium acnes , culture was carried out in a BHI liquid medium for 48 hours. The bacterial culture prepared by the above method was diluted to a proper concentration in physiological saline, and 0.1 ml of each of 0.1 ml of BHI solid medium And then dried. Each of the dry powders of Example 1 and Example 2 was diluted to 5% concentration in sterilized saline, and 0.5 ml of the dried powder was dispensed on the prepared solid medium. The cells were incubated for 3 days in an anaerobic incubator at 35 ° C. ) Were measured. As a control group, 0.5 ml of the sterilized saline solution was dispensed on a solid medium, and the cells were incubated in a 35 ° C anaerobic incubation tank for 3 days. The number of colonies (N1) was measured and the respective sterilization powers were calculated by the following formula The results are shown in Table 3 below.

[Equation 2]

Sterilization rate (%) for acne bacteria = (N1-N2) / N1 100

[Table 3] Sterilization rate of acne bacterium

Name of sample N1 N2 Sterilization rate (%) Example 1 790 64 91.9 Example 2 790 68 91.4 Example 1 + Example 2 790 61 92.3

As can be seen from Table 3, it was confirmed that each of the extracts of the present invention had a germicidal effect against acne bacteria of 90% or more and exhibited excellent acne bactericidal effect.

Formulation Example 1. Lotion Production

[Table 4] Lotion Composition

ingredient Content (% by weight) Major + retirement 1.00 Liquid paraffin 5.00 Caprylic / capric triglyceride 5.00 Wax 4.00 glycerin 3.00 Butylene glycol 3.00 Propylene glycol 3.00 Polysorbate 1.50 Sorbitan sesquioleate 0.70 Carboxyvinyl polymer 0.10 Triethanolamine 0.20 antiseptic Suitable amount Pigment Suitable amount Spices Suitable amount Purified water to 100

Lotion was prepared with the composition shown in Table 4 above.

Test Example 3. Effect of suppressing sebum secretion and alleviating acne

Ten men and women who felt that sebum secretion was large on the face apart from 10 men and women who were suffering from acne were selected, and the place of acne and face sebum secretion for 4 weeks were made every day by the prescription of Formulation Example 1 above.

The effectiveness of the test was scored according to the degree of the testee 's feelings and the inhibition of sebum secretion was measured using a sebum analyzer. The results of inhibition of sebum secretion are shown in Table 5, and the acne mitigation effect is shown in Table 6.

[Table 5] Effect of suppressing sebum secretion

Tester Pre-test fatigue (㎍ / ㎠h) Test 2 and after Exam 4 and after One 77 65 63 2 55 38 43 3 35 43 45 4 88 71 62 5 71 65 55 6 23 33 38 7 48 43 44 8 65 40 46 9 79 66 61

[Table 6] Effect of alleviating acne

Tester
Pre-test condition
Effect determination Two weeks after the test After 4 weeks of testing One One - + 2 3 ++ ++ 3 2 + ++ 4 One + + 5 2 - + 6 3 - ++ 7 2 ++ +++ 8 One + ++ 9 2 + + 10 2 + ++ * Pre-test status: 1 - a little acne 2 - acne moderate 3 - acne very severe
* Effect determination; -: Little effect +: Little effect
++: Very effective +++: Very effective (Almost healed)

From the results of Tables 5 and 6, it can be seen that the composition according to the present invention can inhibit sebum and thus alleviate acne.

As described above, the cosmetic composition containing the extract of the present invention or the extract of the present invention suppresses the activity of 5-alpha-reductase to inhibit the production of active male hormone, By suppressing the growth of ness, sebum secretion can be suppressed and acne can be improved. In addition, since the composition of the present invention contains a natural herbal extract, it can be safely used without long term use without side effects.

Claims (9)

A cosmetic composition for suppressing sebum and improving acne containing a dipole extract as an active ingredient. A cosmetic composition for suppressing sebum and improving acne, comprising the extract as an active ingredient. A cosmetic composition for improving sebum suppression and improving acne, comprising as an active ingredient, a mixed extract of herbal medicines comprising 0.01 to 1: The cosmetic composition for improving sebum suppression and acne according to any one of claims 1 to 3, wherein the extract is contained in an amount of 0.001 to 10% by weight, based on the total weight of the cosmetic composition. delete And a step of concentrating the extract obtained by adding 1 to 5 times by volume of an extraction solvent to the mixture of the counter electrode and the repetition of 0.01 to 1: 1 to 0.01 time by weight, heating or cooling the mixture, and then removing the residue. Gt; 7. The method of claim 6, wherein the extraction solvent is one or more of a lower alcohol having 1 to 5 carbon atoms, hexane, ethyl acetate, and diethyl ether. 7. The method of claim 6, wherein the heating is performed at a temperature of 50 to 100 DEG C for 1 to 10 hours. [7] The method according to claim 6, wherein the cold-rolling is performed at a temperature of 0 to 40 DEG C for 1 to 30 days.