CN114455998A - Wine grape yellowing disease correction biological agent and preparation method and use method thereof - Google Patents
Wine grape yellowing disease correction biological agent and preparation method and use method thereof Download PDFInfo
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- 235000014787 Vitis vinifera Nutrition 0.000 title claims abstract description 139
- 240000006365 Vitis vinifera Species 0.000 title claims abstract description 129
- 238000004383 yellowing Methods 0.000 title claims abstract description 64
- 239000003124 biologic agent Substances 0.000 title claims abstract description 46
- 201000010099 disease Diseases 0.000 title claims abstract description 38
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 38
- 238000002360 preparation method Methods 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 23
- 241000196324 Embryophyta Species 0.000 claims abstract description 45
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims abstract description 42
- 239000002002 slurry Substances 0.000 claims abstract description 42
- 239000002699 waste material Substances 0.000 claims abstract description 24
- 239000007788 liquid Substances 0.000 claims abstract description 23
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000006227 byproduct Substances 0.000 claims abstract description 18
- 229910052742 iron Inorganic materials 0.000 claims abstract description 16
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 14
- 150000002910 rare earth metals Chemical class 0.000 claims abstract description 14
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000011591 potassium Substances 0.000 claims abstract description 13
- 229910052700 potassium Inorganic materials 0.000 claims abstract description 13
- 239000000843 powder Substances 0.000 claims abstract description 13
- YTSDVWYUJXKXCC-UHFFFAOYSA-L zinc;2-[2-[carboxylatomethyl(carboxymethyl)amino]ethyl-(carboxymethyl)amino]acetate Chemical compound [Zn+2].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O YTSDVWYUJXKXCC-UHFFFAOYSA-L 0.000 claims abstract description 13
- 238000003973 irrigation Methods 0.000 claims description 48
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- 239000012528 membrane Substances 0.000 claims description 6
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- 238000009395 breeding Methods 0.000 claims description 4
- 230000001488 breeding effect Effects 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 4
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000010413 mother solution Substances 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 238000003756 stirring Methods 0.000 claims description 4
- ZOXJGFHDIHLPTG-UHFFFAOYSA-N Boron Chemical compound [B] ZOXJGFHDIHLPTG-UHFFFAOYSA-N 0.000 claims description 3
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims description 3
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims description 3
- 150000001413 amino acids Chemical class 0.000 claims description 3
- 229910052796 boron Inorganic materials 0.000 claims description 3
- 239000011575 calcium Substances 0.000 claims description 3
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- 239000011777 magnesium Substances 0.000 claims description 3
- 229910052749 magnesium Inorganic materials 0.000 claims description 3
- BEZDDPMMPIDMGJ-UHFFFAOYSA-N pentamethylbenzene Chemical compound CC1=CC(C)=C(C)C(C)=C1C BEZDDPMMPIDMGJ-UHFFFAOYSA-N 0.000 claims description 3
- 230000008569 process Effects 0.000 claims description 3
- 239000011782 vitamin Substances 0.000 claims description 3
- 235000013343 vitamin Nutrition 0.000 claims description 3
- 229940088594 vitamin Drugs 0.000 claims description 3
- 229930003231 vitamin Natural products 0.000 claims description 3
- 210000003278 egg shell Anatomy 0.000 claims description 2
- 238000005516 engineering process Methods 0.000 claims description 2
- 238000000855 fermentation Methods 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- 150000002989 phenols Chemical class 0.000 claims description 2
- -1 pyroligneous liquor Substances 0.000 claims description 2
- 238000009287 sand filtration Methods 0.000 claims description 2
- 206010047642 Vitiligo Diseases 0.000 claims 1
- 230000002550 fecal effect Effects 0.000 claims 1
- 230000012010 growth Effects 0.000 abstract description 10
- 235000016709 nutrition Nutrition 0.000 abstract description 5
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- 230000002195 synergetic effect Effects 0.000 abstract description 4
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- 235000015816 nutrient absorption Nutrition 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 17
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- 239000002689 soil Substances 0.000 description 7
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- 239000000203 mixture Substances 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N phenol group Chemical group C1(=CC=CC=C1)O ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
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- 238000009472 formulation Methods 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
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- 239000004410 anthocyanin Substances 0.000 description 2
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- PRPINYUDVPFIRX-UHFFFAOYSA-N 1-naphthaleneacetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CC=CC2=C1 PRPINYUDVPFIRX-UHFFFAOYSA-N 0.000 description 1
- GHRYSOFWKRRLMI-UHFFFAOYSA-N 1-naphthyloxyacetic acid Chemical compound C1=CC=C2C(OCC(=O)O)=CC=CC2=C1 GHRYSOFWKRRLMI-UHFFFAOYSA-N 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
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- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 206010048259 Zinc deficiency Diseases 0.000 description 1
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- 239000003899 bactericide agent Substances 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- NKWPZUCBCARRDP-UHFFFAOYSA-L calcium bicarbonate Chemical compound [Ca+2].OC([O-])=O.OC([O-])=O NKWPZUCBCARRDP-UHFFFAOYSA-L 0.000 description 1
- 229910000020 calcium bicarbonate Inorganic materials 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 229930002869 chlorophyll b Natural products 0.000 description 1
- NSMUHPMZFPKNMZ-VBYMZDBQSA-M chlorophyll b Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C=O)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 NSMUHPMZFPKNMZ-VBYMZDBQSA-M 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
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- 239000011574 phosphorus Substances 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/02—Cultivation of hops or vines
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Botany (AREA)
- Soil Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Medicines Containing Plant Substances (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a wine grape yellowing disease correction biological agent and a preparation method and a use method thereof, wherein the wine grape yellowing disease correction biological agent comprises the following components in parts by weight: 100 parts of biogas slurry, 11-19 parts of biogas slurry concentrated byproducts, 1-5 parts of waste egg liquid, 1-9 parts of pyroligneous liquor, 1-5 parts of potassium humate, 1-5 parts of citric acid, 0.1-0.5 part of EDTA-zinc, 0.1-0.5 part of EDDHA-iron and 0.1-0.3 part of rare earth powder. According to the invention, the wine grape yellowing correction biological agent is utilized, and under the mutual synergistic effect of multiple effective components, through the establishment of root zone environment, the growth promotion and resistance of root systems, the addition of external nutrition sources, the fixed activation and the supplement and matching of leaf nutrition, a good root system structure is constructed, the smooth nutrient absorption and operation are promoted, and the problem of plant yellowing is solved.
Description
Technical Field
The invention relates to the technical field of biological preparations for wine grapes, and particularly relates to a wine grape yellowing correction biological preparation and a preparation method and a use method thereof.
Background
The yellow flower disease is the phenomenon that leaves and partial expansion period fruits are yellowed and even white flower appears due to the fact that chlorophyll synthesis is blocked in the growth process of fruit trees. The formation of the yellow mosaic disease is mainly caused by the fact that the synthesis of chlorophyll is blocked due to root system morbidity, and the planting of the wine grapes is important for the correction of the yellow mosaic disease.
In northwest arid and semi-arid regions, the matrix of the formed soil is mainly loess-shaped sediments and alluvial deposits, the soil is highly saturated with salt base and is in alkaline reaction, and the formed soil is rich in calcareous substances such as calcium carbonate or calcium bicarbonate, so that the symptoms of plant malnutrition, iron deficiency, phosphorus deficiency, zinc deficiency and the like are easily caused; meanwhile, the high-concentration bicarbonate can inhibit the growth of plant root systems, so that the root system loss is caused, the absorption and operation of water and nutrients are hindered, and the healthy growth of crops is influenced.
In arid and semi-arid regions in northwest, the wine grape leaf yellowing is a physiological disease commonly occurring in wine grapes, the direct cause of the physiological disease is caused by imbalance of required nutrient elements, and the indirect cause is linkage reaction caused by soil barrier problem, so that plant yellowing and even dying is caused, and the healthy and high-quality development of the wine grape industry is seriously restricted.
In view of this, the research and development of the wine grape yellowing disease correction biological agent, the preparation method and the application method thereof are significant.
Disclosure of Invention
The invention aims to solve the technical problem of providing a wine grape yellowing disease correcting biological preparation and a preparation method and a using method thereof.
The invention provides a wine grape yellowing disease correction biological agent, which comprises the following components in parts by weight: 100 parts of biogas slurry, 11-19 parts of biogas slurry concentrated byproducts, 1-5 parts of waste egg liquid, 1-9 parts of pyroligneous liquor, 1-5 parts of potassium humate, 1-5 parts of citric acid, 0.1-0.5 part of EDTA-zinc, 0.1-0.5 part of EDDHA-iron and 0.1-0.3 part of rare earth powder.
Preferably, the composition comprises the following components in parts by weight: 100 parts of biogas slurry, 15 parts of biogas slurry concentrated byproducts, 3 parts of waste egg liquid, 5 parts of pyroligneous liquor, 3 parts of potassium humate, 2.5 parts of citric acid, 0.3 part of EDTA-zinc, 0.3 part of EDDHA-iron and 0.2 part of rare earth powder.
Preferably, the biogas slurry is 5 times of concentrated solution of the anaerobic fermentation biogas slurry of the waste eggs and the feces, and the effective components in the biogas slurry comprise: trace elements, methyl naphthylacetate and phenols, wherein the trace elements comprise calcium, magnesium, iron and boron.
Preferably, the effective components in the biogas slurry concentration by-product comprise: naphthalene, pentamethylbenzene and 2-methylnaphthalene.
Preferably, the effective components in the waste egg liquid comprise: amino acids and vitamins.
The invention also provides a preparation method of the wine grape yellowing disease correction biological agent, which comprises the following steps:
(1) naturally settling waste eggs and excrement generated in laying hen breeding, then performing sand filtration and security filtration, then feeding into a concentrated liquid tank, pressurizing by a pump, and feeding into an ultrafiltration membrane separator for concentration and separation to obtain biogas slurry;
(2) in the process of ultrafiltration membrane separation technology, a biogas slurry concentrated by-product is obtained through trapped liquid generated by an ultrafiltration membrane separator;
(3) stirring waste eggs produced by laying hen breeding and filtering eggshells to obtain waste egg liquid;
(4) taking the biogas slurry as a mother solution, sequentially adding a certain amount of biogas slurry concentrated byproducts, pyroligneous liquor, potassium humate, waste egg liquid, citric acid, EDTA-zinc, EDDHA-iron and rare earth powder into the mother solution, and uniformly stirring and mixing to obtain the vintage grape old and young tree correcting biological preparation.
The invention also provides a use method of the wine grape yellowing disease correction biological agent, which comprises the following steps:
(1) the first year is used: in the first year, aiming at a etiolated plant or etiolated region, the vintage grape small old tree correction biological agent is applied to the vintage grape in a pit digging root irrigation mode;
(2) subsequent application: and beginning in the second year, applying the biological agent for correcting the old grape vine by adopting a mode of combining root irrigation and foliage spraying for 2-3 years, irrigating the wine grape vine for 3-4 times at the root of each year, and spraying the biological agent for correcting the old grape vine for 3-4 times on the foliage of the same period of the root irrigation.
Preferably, the application method in the step (1) is as follows:
in the first year: irrigating the wine grapes for 1 time respectively in the young sprout period and the autumn sprout period; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, diluting and then pouring the diluted solution into tree pits according to the amount of 2-3 kg/plant.
Preferably, the application method in the step (2) is as follows:
in the second year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after diluting, filling the plant into tree pits according to the amount of 2-3 kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: spraying the daylily plants to the leaf surfaces for 3-4 times after 300 dilution;
third year and fourth year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug in the diagonal line of the root irrigation position in the last year in a staggered mode, and the biological preparation for correcting wine grape yellowing and water are 1: 5, after diluting, filling the plant into tree pits according to the amount of 2-3 kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: and after 300 times of dilution, spraying the daylily plants to the leaf surfaces for 3-4 times.
The principle of the invention is as follows: the wine grape yellowing disease correction biological preparation comprises the following components in parts by weight: 100 parts of biogas slurry, 11-19 parts of biogas slurry concentrated byproducts, 1-9 parts of pyroligneous liquor, 1-5 parts of potassium humate, 1-5 parts of waste egg liquid, 1-5 parts of citric acid, 0.1-0.5 part of EDTA-zinc, 0.1-0.5 part of EDDHA-iron and 0.1-0.3 part of rare earth powder, wherein the components are as follows:
the biogas slurry is 5 times of biogas slurry concentrated solution, wherein the biogas slurry is rich in medium and trace elements such as calcium, magnesium, iron and boron, and can provide rich medium and trace nutrients for root growth; meanwhile, the plant growth regulator is rich in methyl naphthylacetate and a large amount of phenolic substances, wherein the methyl naphthylacetate can be directly absorbed by plant roots, stems and leaves and is a plant growth regulator with auxin activity; phenolic substances are important raw materials of bactericides, preservatives and insecticides;
the biogas slurry concentrated byproduct is rich in plant source chemical substances such as naphthalene, pentamethylbenzene, 2-methylnaphthalene and the like, is an intermediate of plant growth regulators such as naphthylacetic acid, naphthyloxyacetic acid and the like, has remarkable root promoting, rooting and sterilizing effects, and can promote the growth of wine grape root systems;
the addition of the citric acid can adjust the soil pH value of the root zone, break the soil calcium deposit layer, activate the soil to hold nutrients and construct a root system development space;
the waste egg liquid is rich in various beneficial substances such as amino acids, vitamins and the like, and provides nutrition and growth power for the growth and development of grape root systems;
the trace rare earth can enhance the life activity of plant roots and is an effective plant growth regulator.
The invention has the beneficial effects that: under the mutual synergistic action of various components, the wine grape yellowing correction biological agent constructs a good root system structure through the establishment of a root zone environment, the growth promotion and stress resistance of the root system, the addition of a nutrient external source, the fixed activation and the supplement and matching of leaf nutrition, promotes the smooth absorption and operation of nutrients, and solves the problem of plant yellowing.
Detailed Description
In order to make the technical solution of the present invention easier to understand, the technical solution of the present invention is now clearly and completely described by using the specific embodiments.
Example 1:
the wine grape yellowing disease correction biological agent comprises the following components in parts by weight: 100 parts of biogas slurry, 15 parts of biogas slurry concentrated byproducts, 3 parts of waste egg liquid, 5 parts of pyroligneous liquor, 3 parts of potassium humate, 2.5 parts of citric acid, 0.3 part of EDTA-zinc, 0.3 part of EDDHA-iron and 0.2 part of rare earth powder.
The application method of the wine grape yellowing disease correction biological agent comprises the following steps:
in the first year: irrigating the wine grapes for 1 time respectively in the young sprout period and the autumn sprout period; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5 diluting the plant, and then filling the plant into tree pits according to the amount of 2.5kg per plant;
in the second year: root irrigation is carried out for 1 time in the green turning period, 2 times in the expansion period and 1 time in the coloring period of the wine grapes; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5 diluting the plant, and then filling the plant into tree pits according to the amount of 2.5kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: 300, spraying yellow flower plants on the leaf surfaces for 4 times;
third year and fourth year: root irrigation is carried out for 1 time in the green turning period, 2 times in the expansion period and 1 time in the coloring period of the wine grapes; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug in the diagonal line of the root irrigation position in the last year in a staggered mode, and the biological preparation for correcting wine grape yellowing and water are 1: 5 diluting the plant, and then filling the plant into tree pits according to the amount of 2.5kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: after 300 dilution, spraying the day lily plants on the leaf surfaces for 4 times.
Example 2:
the wine grape yellowing disease correction biological agent comprises the following components in parts by weight: 100 parts of biogas slurry, 11 parts of biogas slurry concentrated byproducts, 1 part of waste egg liquid, 1 part of pyroligneous liquor, 1 part of potassium humate, 1 part of citric acid, 0.1 part of EDTA-zinc, 0.1 part of EDDHA-iron and 0.1 part of rare earth powder.
The application method of the wine grape yellowing disease correction biological agent comprises the following steps:
in the first year: irrigating the wine grapes for 1 time respectively in the young sprout period and the autumn sprout period; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after diluting, irrigating the plant into tree pits according to the amount of 2kg per plant;
in the second year: root irrigation is carried out for 1 time in the green turning period, 2 times in the expansion period and 1 time in the coloring period of the wine grapes; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after diluting, irrigating the plant into tree pits according to the amount of 2kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: 300, spraying yellow flower plants on the leaf surfaces for 4 times;
in the third year: root irrigation is carried out for 1 time in the green turning period, 1 time in the expansion period and 1 time in the coloring period of the wine grapes; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug in the diagonal line of the root irrigation position in the last year in a staggered mode, and the biological preparation for correcting wine grape yellowing and water are 1: 5, after diluting, irrigating the plant into tree pits according to the amount of 2kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: 300 dilution, spraying the daylily plant on the leaf surface for 3 times.
Example 3:
the wine grape yellowing disease correction biological agent comprises the following components in parts by weight: 100 parts of biogas slurry, 19 parts of biogas slurry concentrated byproducts, 5 parts of waste egg liquid, 9 parts of pyroligneous liquor, 5 parts of potassium humate, 5 parts of citric acid, 0.5 part of EDTA-zinc, 0.5 part of EDDHA-iron and 0.3 part of rare earth powder.
The application method of the wine grape yellowing disease correction biological agent comprises the following steps:
in the first year: irrigating the wine grapes respectively for 1 time in the young sprout period and the autumn sprout period; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after dilution, irrigating the plant into tree pits according to the amount of 3kg per plant;
in the second year: root irrigation is carried out for 1 time in the green turning period, 1 time in the expansion period and 1 time in the coloring period of the wine grapes; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after dilution, irrigating the plant into tree pits according to the amount of 3kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: 300 diluting, and spraying daylily plants to the leaf surfaces for 3 times;
third year and fourth year: root irrigation is carried out for 1 time in the green turning period, 1 time in the expansion period and 1 time in the coloring period of the wine grapes; outside the root irrigation position 20cm away from the main grape vine stem, a fan-shaped tree pit with the depth of 30cm is dug in the root irrigation position in the last year in a staggered mode, and the wine grape yellowing disease correction biological agent and water are mixed according to the weight ratio of 1: 5, after dilution, irrigating the plant into tree pits according to the amount of 3kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: 300 dilution, spraying the daylily plant on the leaf surface for 3 times.
Comparative example 1:
the wine grape yellowing disease correction biological preparation comprises the following components in parts by weight: 1 part of pyroligneous, 1 part of potassium humate, 1 part of citric acid, 0.1 part of EDTA-zinc, 0.1 part of EDDHA-iron and 0.1 part of rare earth powder.
The application method of the wine grape yellowing correction biological agent of the comparative example is the same as that of example 1.
Comparative example 2:
the wine grape yellowing disease correction biological preparation comprises the following components in parts by weight: 100 parts of biogas slurry, 11 parts of a biogas slurry concentrated by-product, 1 part of waste egg liquid, 0.1 part of EDTA-zinc, 0.1 part of EDDHA-iron and 0.1 part of rare earth powder.
The application method of the wine grape yellowing correction biological agent of the comparative example is the same as that of example 1.
Comparative example 3:
the wine grape yellowing disease correction biological preparation comprises the following components in parts by weight: 100 parts of biogas slurry, 11 parts of biogas slurry concentrated byproducts, 1 part of waste egg liquid, 1 part of wood vinegar liquid, 1 part of potassium humate and 1 part of citric acid.
The application method of the wine grape yellowing correction biological agent of the comparative example is the same as that of example 1.
Comparative example 4:
in the comparative example, the treatment of wine grapes comprises the following steps:
in the first year: irrigating the wine grapes for 1 time respectively in the young sprout period and the autumn sprout period; digging a 30 cm-deep fan-shaped tree pit at the diagonal position of a root irrigation position 20cm away from the main stem of the grape tree, and irrigating water into the tree pit according to the amount of 2-3 kg per plant;
in the second year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; digging a 30 cm-deep fan-shaped tree pit at the diagonal position of a root irrigation position 20cm away from the main stem of the grape tree, and irrigating water into the tree pit according to the amount of 2-3 kg per plant; spraying the daylily plants to the leaf surfaces for 3-4 times by using water in the root irrigation period;
third year and fourth year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug in the root irrigation position in the last year in a staggered mode, and water is irrigated into the tree pit according to the amount of 2-3 kg per tree; and spraying the daylily plants to the leaf surfaces for 3-4 times by using water in the root irrigation period.
TABLE 1 Effect of different formulations on photosynthetic pigments and enzymatic Activity in leaves of Vitis vinifera
Table 1 can be found: compared with the blank control treatment in the comparative example 4, the contents of chlorophyll a, chlorophyll b, carotenoid, catalase and polyphenol oxidase in the leaves can be obviously increased in the examples 1 to 3. The wine grape yellowing correction biological preparation formula in the embodiment 1 has the best effect, and the chlorophyll a content is respectively increased by 31.55% and 18.84% compared with the contents in the embodiments 2 and 3; compared with the carotenoid content of 47.37 percent and 21.74 percent respectively in examples 2 and 3; examples 1 and 3 had little effect on catalase activity and polyphenol oxidase activity, but were significantly higher than in example 2. Meanwhile, compared with the blank control treatment in the comparative example 4, the blank control treatment in the comparative examples 1 to 3 has certain influence on the photosynthetic pigments and the enzyme activity of the leaves of the grapes for brewing, but the improvement on the indexes is limited and is far lower than that in the examples 1 to 3.
TABLE 2 Effect of different formulations on photosynthetic parameters of leaves of Vitis vinifera
Table 2 can be obtained; compared with the white control treatment in the comparative example 4, the net photosynthetic rate is remarkably improved in the examples 1 to 3, wherein the effect of the example 1 is most obvious, and 65.24% and 36.50% are respectively increased in the examples 2 and 3; transpiration rate, intercellular CO2The concentration is reduced under the treatment of the embodiments 1-3, but the effective utilization rate of the water is obviously improved, and the effective utilization rate of the water under the treatment of the embodiment 1 is increased by 1.93 times compared with the white contrast treatment in the contrast ratio 4; example 1, which had the greatest porosity conductance, was followed by example 3, which increased 14.58% and 8.33% respectively over example 2, and 72.14% and 58.90% respectively over the white control treatment of comparative example 4. Meanwhile, compared with the blank control treatment in the comparative example 4, the blank control treatment in the comparative examples 1-3 has certain influence on the photosynthetic parameters of the leaves of the wine grapes, but the improvement on the indexes is limited and is far lower than that in the examples 1-3.
TABLE 3 influence of different formulations on the yield and quality of wine grapes
Table 3 may be obtained; compared with the white-air contrast treatment in the comparative example 4, the yields of the wine grapes in the examples 1-3 are respectively and remarkably increased by 2.93 times, 2.55 times and 2.65 times, the yield of the example 1 is the highest, and the yields of the wine grapes in the examples 2 and 3 are respectively increased by 14.66% and 10.40%; example 1 also has an obvious effect on quality improvement, the content of soluble solids is obviously increased, the content of titratable acid is reduced, and the sugar-acid ratio is improved by 26.68% compared with comparative example 4 in the air-white control treatment; examples 1 to 3 can obviously reduce the tannin content and increase the contents of anthocyanin and total phenol, wherein the anthocyanin content is the highest under the treatment of example 1, and is increased by 17.78% and 19.46% respectively compared with examples 2 and 3, and the influence effect of example 1 and example 3 on the total phenol is basically consistent, and is increased by 5.69% and 4.90% respectively compared with example 2. Meanwhile, compared with the blank control treatment in the comparison ratio 4, the blank control treatment in the comparison ratios 1 to 3 has certain influence on the yield and the quality of the wine grapes, but the improvement on the indexes is limited and is far lower than that in the embodiments 1 to 3.
In conclusion, the wine grape yellowing correction biological agent is used for treating the wine grapes, so that the growth of the wine grapes and the grape quality can be effectively improved, and the improvement of the growth of the wine grapes and the product indexes after partial components are removed from the agent is extremely limited, so that the improvement effect of the wine grape yellowing correction biological agent on the wine grapes can not be achieved. Therefore, the ingredients of the yellows correcting biological preparation have a synergistic effect, and under the mutual synergistic effect of the ingredients, a good root system structure is constructed through establishment of root zone environment, growth promotion and stress resistance of the root system, addition and fixed activation of nutrient external sources and supplement and cooperation of leaf nutrition, so that smooth nutrient absorption and operation are promoted, and the problem of plant yellowing is solved.
It should be noted that the embodiments described herein are only some embodiments of the present invention, and not all implementations of the present invention, and the embodiments are only examples, which are only used to provide a more intuitive and clear understanding of the present invention, and are not intended to limit the technical solutions of the present invention. All other embodiments, as well as other simple substitutions and various changes to the technical solutions of the present invention, which can be made by those skilled in the art without inventive work, are within the scope of the present invention without departing from the spirit of the present invention.
Claims (9)
1. The wine grape yellowing disease correction biological agent is characterized by comprising the following components in parts by weight: 100 parts of biogas slurry, 11-19 parts of biogas slurry concentrated byproducts, 1-5 parts of waste egg liquid, 1-9 parts of pyroligneous liquor, 1-5 parts of potassium humate, 1-5 parts of citric acid, 0.1-0.5 part of EDTA-zinc, 0.1-0.5 part of EDDHA-iron and 0.1-0.3 part of rare earth powder.
2. The viniferous yellows correction biological agent as claimed in claim 1, comprising the following components in parts by weight: 100 parts of biogas slurry, 15 parts of biogas slurry concentrated byproducts, 3 parts of waste egg liquid, 5 parts of pyroligneous liquor, 3 parts of potassium humate, 2.5 parts of citric acid, 0.3 part of EDTA-zinc, 0.3 part of EDDHA-iron and 0.2 part of rare earth powder.
3. The wine grape yellowing correction biological agent according to claim 1 or 2, wherein the biogas slurry is a 5-fold concentrated solution of waste egg and fecal anaerobic fermentation biogas slurry, and the effective components in the biogas slurry comprise: trace elements, methyl naphthylacetate and phenols, wherein the trace elements comprise calcium, magnesium, iron and boron.
4. The wine grape yellowing correction biological agent according to claim 1 or 2, wherein the biogas slurry concentration by-product comprises the following effective components: naphthalene, pentamethylbenzene and 2-methylnaphthalene.
5. The wine grape yellowing correction biological agent according to claim 1 or 2, wherein the effective components in the waste egg liquid comprise: amino acids and vitamins.
6. The preparation method of the wine grape yellowing disease correction biological agent is characterized by comprising the following steps:
(1) naturally settling waste eggs and excrement generated in laying hen breeding, then performing sand filtration and security filtration, then feeding into a concentrated liquid tank, pressurizing by a pump, and feeding into an ultrafiltration membrane separator for concentration and separation to obtain biogas slurry;
(2) in the process of ultrafiltration membrane separation technology, a biogas slurry concentrated by-product is obtained through trapped liquid generated by an ultrafiltration membrane separator;
(3) stirring waste eggs produced by laying hen breeding and filtering eggshells to obtain waste egg liquid;
(4) taking the biogas slurry as a mother solution, sequentially adding a certain amount of biogas slurry concentrated byproducts, pyroligneous liquor, potassium humate, waste egg liquid, citric acid, EDTA-zinc, EDDHA-iron and rare earth powder into the mother solution, and uniformly stirring and mixing to obtain the vintage grape old and young tree correcting biological preparation.
7. The application method of the wine grape yellowing disease correction biological agent is characterized by comprising the following steps:
(1) the first year is used: in the first year, aiming at a etiolated plant or etiolated region, the vintage grape small old tree correction biological agent is applied to the vintage grape in a pit digging root irrigation mode;
(2) subsequent application: and beginning in the second year, applying the biological agent for correcting the old grape vine by adopting a mode of combining root irrigation and foliage spraying for 2-3 years, irrigating the wine grape vine for 3-4 times at the root of each year, and spraying the biological agent for correcting the old grape vine for 3-4 times on the foliage of the same period of the root irrigation.
8. The method for using the vintage old tree biological preparation as claimed in claim 7, wherein the application method in step (1) is as follows:
in the first year: irrigating the wine grapes for 1 time respectively in the young sprout period and the autumn sprout period; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, diluting and then pouring the diluted solution into tree pits according to the amount of 2-3 kg/plant.
9. The use method of the vitiligo correction biological agent according to claim 7, wherein the application method in the step (2) is as follows:
in the second year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug at the diagonal position, and the biological preparation for correcting wine grape yellowing and water are mixed according to the weight ratio of 1: 5, after diluting, filling the plant into tree pits according to the amount of 2-3 kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: spraying the daylily plants to the leaf surfaces for 3-4 times after 300 dilution;
third year and fourth year: irrigating the wine grapes for 1 time at the green turning stage, irrigating the wine grapes for 1-2 times at the swelling stage and irrigating the wine grapes for 1 time at the coloring stage; the root irrigation position is 20cm away from the main stem of the grape vine, a fan-shaped tree pit with the depth of 30cm is dug in the diagonal line of the root irrigation position in the last year in a staggered mode, and the biological preparation for correcting wine grape yellowing and water are 1: 5, after diluting, filling the plant into tree pits according to the amount of 2-3 kg per plant; the wine grape yellowing disease correction biological agent and water used in the root irrigation synchronization are mixed according to the weight ratio of 1: and after 300 times of dilution, spraying the daylily plants to the leaf surfaces for 3-4 times.
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