CN114450418A - 细胞的分化状态的评价方法 - Google Patents

细胞的分化状态的评价方法 Download PDF

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Publication number
CN114450418A
CN114450418A CN201980098491.9A CN201980098491A CN114450418A CN 114450418 A CN114450418 A CN 114450418A CN 201980098491 A CN201980098491 A CN 201980098491A CN 114450418 A CN114450418 A CN 114450418A
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cells
differentiation
amount
cell
pluripotent stem
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Pending
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CN201980098491.9A
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Chinese (zh)
Inventor
原圭祐
五味慎一
永田智久
加川健一
大岛康弘
铃木崇
高桥雅俊
山本贵子
川真田伸
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Cytokines Co ltd
Shimadzu Corp
Tokyo Electron Ltd
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Shimadzu Corp
Tokyo Electron Ltd
Foundation for Biomedical Research and Innovation at Kobe
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
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  • Developmental Biology & Embryology (AREA)
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  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
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  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
CN201980098491.9A 2019-07-19 2019-07-19 细胞的分化状态的评价方法 Pending CN114450418A (zh)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/028556 WO2021014515A1 (ja) 2019-07-19 2019-07-19 細胞の分化状態の評価方法

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CN114450418A true CN114450418A (zh) 2022-05-06

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CN201980098491.9A Pending CN114450418A (zh) 2019-07-19 2019-07-19 细胞的分化状态的评价方法

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US (1) US20220260580A1 (https=)
EP (1) EP4001425A4 (https=)
JP (1) JP7344421B2 (https=)
CN (1) CN114450418A (https=)
TW (1) TWI855102B (https=)
WO (1) WO2021014515A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114846151A (zh) * 2020-01-17 2022-08-02 株式会社日立高新技术 细胞分化状态的评价方法以及细胞培养系统

Citations (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2013065914A1 (ko) * 2011-11-02 2013-05-10 제일약품 주식회사 낭성구조물로부터 망막색소상피세포로의 분화 유도 방법
US20130224156A1 (en) * 2012-02-29 2013-08-29 Riken Method of producing human retinal pigment epithelial cells
EP2644694A1 (en) * 2010-10-28 2013-10-02 National University Corporation Kumamoto University Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
CN104946591A (zh) * 2007-10-12 2015-09-30 奥卡塔治疗公司 制备rpe 细胞和rpe 细胞的组合物的改良方法
CN105814194A (zh) * 2013-12-11 2016-07-27 辉瑞有限公司 产生视网膜色素上皮细胞的方法
CN106460028A (zh) * 2014-05-01 2017-02-22 株式会社岛津制作所 细胞的分化状态的评价方法
WO2017068727A1 (ja) * 2015-10-23 2017-04-27 株式会社島津製作所 細胞の分化状態の評価方法
CN106609256A (zh) * 2015-10-22 2017-05-03 同济大学 体外诱导人胚胎干细胞分化为视网膜色素上皮细胞的方法
CN108350421A (zh) * 2015-09-08 2018-07-31 大日本住友制药株式会社 用于制备视网膜色素上皮细胞的方法

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
KR102254082B1 (ko) 2012-08-24 2021-05-18 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 망막 색소 상피 세포 시트의 제조 방법

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104946591A (zh) * 2007-10-12 2015-09-30 奥卡塔治疗公司 制备rpe 细胞和rpe 细胞的组合物的改良方法
EP2644694A1 (en) * 2010-10-28 2013-10-02 National University Corporation Kumamoto University Method and culture medium for improving pluripotent stem cell differentiation inducing efficiency
WO2013065914A1 (ko) * 2011-11-02 2013-05-10 제일약품 주식회사 낭성구조물로부터 망막색소상피세포로의 분화 유도 방법
US20130224156A1 (en) * 2012-02-29 2013-08-29 Riken Method of producing human retinal pigment epithelial cells
CN105814194A (zh) * 2013-12-11 2016-07-27 辉瑞有限公司 产生视网膜色素上皮细胞的方法
CN106460028A (zh) * 2014-05-01 2017-02-22 株式会社岛津制作所 细胞的分化状态的评价方法
CN108350421A (zh) * 2015-09-08 2018-07-31 大日本住友制药株式会社 用于制备视网膜色素上皮细胞的方法
CN106609256A (zh) * 2015-10-22 2017-05-03 同济大学 体外诱导人胚胎干细胞分化为视网膜色素上皮细胞的方法
WO2017068727A1 (ja) * 2015-10-23 2017-04-27 株式会社島津製作所 細胞の分化状態の評価方法
CN108350480A (zh) * 2015-10-23 2018-07-31 株式会社岛津制作所 细胞的分化状态的评价方法

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WO2021014515A1 (ja) 2021-01-28
EP4001425A1 (en) 2022-05-25
TWI855102B (zh) 2024-09-11
EP4001425A4 (en) 2023-04-19
TW202117019A (zh) 2021-05-01
JPWO2021014515A1 (https=) 2021-01-28
JP7344421B2 (ja) 2023-09-14
US20220260580A1 (en) 2022-08-18

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