JP7344421B2 - 細胞の分化状態の評価方法 - Google Patents

細胞の分化状態の評価方法 Download PDF

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Publication number
JP7344421B2
JP7344421B2 JP2021534873A JP2021534873A JP7344421B2 JP 7344421 B2 JP7344421 B2 JP 7344421B2 JP 2021534873 A JP2021534873 A JP 2021534873A JP 2021534873 A JP2021534873 A JP 2021534873A JP 7344421 B2 JP7344421 B2 JP 7344421B2
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cells
pluripotent stem
indicator substance
differentiation
stem cells
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JPWO2021014515A1 (https=
JPWO2021014515A5 (https=
Inventor
圭祐 原
慎一 五味
智久 永田
健一 加川
康弘 大島
崇 鈴木
雅俊 高橋
貴子 山本
伸 川真田
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CYTO-FACTO INC.
Shimadzu Corp
Tokyo Electron Ltd
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CYTO-FACTO INC.
Shimadzu Corp
Tokyo Electron Ltd
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0618—Cells of the nervous system
    • C12N5/0621—Eye cells, e.g. cornea, iris pigmented cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/50—Proteins

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Reproductive Health (AREA)
  • Urology & Nephrology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Ophthalmology & Optometry (AREA)
  • Neurology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
JP2021534873A 2019-07-19 2019-07-19 細胞の分化状態の評価方法 Active JP7344421B2 (ja)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/JP2019/028556 WO2021014515A1 (ja) 2019-07-19 2019-07-19 細胞の分化状態の評価方法

Publications (3)

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JPWO2021014515A1 JPWO2021014515A1 (https=) 2021-01-28
JPWO2021014515A5 JPWO2021014515A5 (https=) 2022-03-17
JP7344421B2 true JP7344421B2 (ja) 2023-09-14

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US (1) US20220260580A1 (https=)
EP (1) EP4001425A4 (https=)
JP (1) JP7344421B2 (https=)
CN (1) CN114450418A (https=)
TW (1) TWI855102B (https=)
WO (1) WO2021014515A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114846151A (zh) * 2020-01-17 2022-08-02 株式会社日立高新技术 细胞分化状态的评价方法以及细胞培养系统

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015166845A1 (ja) 2014-05-01 2015-11-05 株式会社島津製作所 細胞の分化状態の評価方法
JP2017504311A (ja) 2013-12-11 2017-02-09 ファイザー・リミテッドＰｆｉｚｅｒ Ｌｉｍｉｔｅｄ 網膜色素上皮細胞を生成する方法
WO2017068727A1 (ja) 2015-10-23 2017-04-27 株式会社島津製作所 細胞の分化状態の評価方法

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
DK2209888T3 (da) * 2007-10-12 2020-01-20 Astellas Inst For Regenerative Medicine Forbedrede fremgangsmåder til fremstilling af rpe-celler og sammensætninger af rpe-celler
WO2012056997A1 (ja) * 2010-10-28 2012-05-03 国立大学法人熊本大学 多能性幹細胞の分化誘導効率を改善するための方法及び培地
KR101357851B1 (ko) * 2011-11-02 2014-02-06 제일약품주식회사 낭성구조물로부터 망막색소상피세포의 분화를 유도하는 방법
US10519422B2 (en) * 2012-02-29 2019-12-31 Riken Method of producing human retinal pigment epithelial cells
KR102254082B1 (ko) 2012-08-24 2021-05-18 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 망막 색소 상피 세포 시트의 제조 방법
SG11201801770VA (en) * 2015-09-08 2018-04-27 Sumitomo Dainippon Pharma Co Ltd Method for producing retinal pigment epithelial cells
CN106609256B (zh) * 2015-10-22 2020-04-07 同济大学 体外诱导人胚胎干细胞分化为视网膜色素上皮细胞的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017504311A (ja) 2013-12-11 2017-02-09 ファイザー・リミテッドＰｆｉｚｅｒ Ｌｉｍｉｔｅｄ 網膜色素上皮細胞を生成する方法
WO2015166845A1 (ja) 2014-05-01 2015-11-05 株式会社島津製作所 細胞の分化状態の評価方法
WO2017068727A1 (ja) 2015-10-23 2017-04-27 株式会社島津製作所 細胞の分化状態の評価方法

Also Published As

Publication number Publication date
WO2021014515A1 (ja) 2021-01-28
EP4001425A1 (en) 2022-05-25
TWI855102B (zh) 2024-09-11
EP4001425A4 (en) 2023-04-19
TW202117019A (zh) 2021-05-01
JPWO2021014515A1 (https=) 2021-01-28
CN114450418A (zh) 2022-05-06
US20220260580A1 (en) 2022-08-18

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