JPWO2021014515A5 - - Google Patents
Download PDFInfo
- Publication number
- JPWO2021014515A5 JPWO2021014515A5 JP2021534873A JP2021534873A JPWO2021014515A5 JP WO2021014515 A5 JPWO2021014515 A5 JP WO2021014515A5 JP 2021534873 A JP2021534873 A JP 2021534873A JP 2021534873 A JP2021534873 A JP 2021534873A JP WO2021014515 A5 JPWO2021014515 A5 JP WO2021014515A5
- Authority
- JP
- Japan
- Prior art keywords
- cells
- abundance
- pluripotent stem
- differentiation
- indicator substance
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000004027 cell Anatomy 0.000 claims 35
- 239000000126 substance Substances 0.000 claims 26
- 238000000034 method Methods 0.000 claims 25
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 22
- 230000004069 differentiation Effects 0.000 claims 19
- 210000000844 retinal pigment epithelial cell Anatomy 0.000 claims 18
- 239000012228 culture supernatant Substances 0.000 claims 17
- 230000024245 cell differentiation Effects 0.000 claims 10
- AHLPHDHHMVZTML-BYPYZUCNSA-N L-Ornithine Chemical compound NCCC[C@H](N)C(O)=O AHLPHDHHMVZTML-BYPYZUCNSA-N 0.000 claims 6
- RHGKLRLOHDJJDR-BYPYZUCNSA-N L-citrulline Chemical compound NC(=O)NCCC[C@H]([NH3+])C([O-])=O RHGKLRLOHDJJDR-BYPYZUCNSA-N 0.000 claims 6
- RHGKLRLOHDJJDR-UHFFFAOYSA-N Ndelta-carbamoyl-DL-ornithine Natural products OC(=O)C(N)CCCNC(N)=O RHGKLRLOHDJJDR-UHFFFAOYSA-N 0.000 claims 6
- AHLPHDHHMVZTML-UHFFFAOYSA-N Orn-delta-NH2 Natural products NCCCC(N)C(O)=O AHLPHDHHMVZTML-UHFFFAOYSA-N 0.000 claims 6
- UTJLXEIPEHZYQJ-UHFFFAOYSA-N Ornithine Natural products OC(=O)C(C)CCCN UTJLXEIPEHZYQJ-UHFFFAOYSA-N 0.000 claims 6
- 229960002173 citrulline Drugs 0.000 claims 6
- 235000013477 citrulline Nutrition 0.000 claims 6
- 230000001939 inductive effect Effects 0.000 claims 6
- 229960003104 ornithine Drugs 0.000 claims 6
- OYIFNHCXNCRBQI-UHFFFAOYSA-N 2-aminoadipic acid Chemical compound OC(=O)C(N)CCCC(O)=O OYIFNHCXNCRBQI-UHFFFAOYSA-N 0.000 claims 4
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims 4
- 210000001671 embryonic stem cell Anatomy 0.000 claims 4
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 claims 4
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 claims 2
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims 2
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 claims 2
- 108010024636 Glutathione Proteins 0.000 claims 2
- 229930010555 Inosine Natural products 0.000 claims 2
- UGQMRVRMYYASKQ-KQYNXXCUSA-N Inosine Chemical compound O[C@@H]1[C@H](O)[C@@H](CO)O[C@H]1N1C2=NC=NC(O)=C2N=C1 UGQMRVRMYYASKQ-KQYNXXCUSA-N 0.000 claims 2
- HXEACLLIILLPRG-YFKPBYRVSA-N L-pipecolic acid Chemical compound [O-]C(=O)[C@@H]1CCCC[NH2+]1 HXEACLLIILLPRG-YFKPBYRVSA-N 0.000 claims 2
- 229960005305 adenosine Drugs 0.000 claims 2
- 230000004663 cell proliferation Effects 0.000 claims 2
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 claims 2
- 229960003180 glutathione Drugs 0.000 claims 2
- 229960003786 inosine Drugs 0.000 claims 2
- HXEACLLIILLPRG-RXMQYKEDSA-N l-pipecolic acid Natural products OC(=O)[C@H]1CCCCN1 HXEACLLIILLPRG-RXMQYKEDSA-N 0.000 claims 2
- 238000004519 manufacturing process Methods 0.000 claims 2
- 238000004949 mass spectrometry Methods 0.000 claims 2
- KIDHWZJUCRJVML-UHFFFAOYSA-N putrescine Chemical compound NCCCCN KIDHWZJUCRJVML-UHFFFAOYSA-N 0.000 claims 2
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 claims 1
- CKTSBUTUHBMZGZ-ULQXZJNLSA-N 4-amino-1-[(2r,4s,5r)-4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-tritiopyrimidin-2-one Chemical compound O=C1N=C(N)C([3H])=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-ULQXZJNLSA-N 0.000 claims 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 claims 1
- XUJNEKJLAYXESH-REOHCLBHSA-N L-Cysteine Chemical compound SC[C@H](N)C(O)=O XUJNEKJLAYXESH-REOHCLBHSA-N 0.000 claims 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims 1
- 239000005700 Putrescine Substances 0.000 claims 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 claims 1
- 235000018417 cysteine Nutrition 0.000 claims 1
- 229960002433 cysteine Drugs 0.000 claims 1
- 210000002919 epithelial cell Anatomy 0.000 claims 1
- 210000004263 induced pluripotent stem cell Anatomy 0.000 claims 1
- 239000000790 retinal pigment Substances 0.000 claims 1
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| PCT/JP2019/028556 WO2021014515A1 (ja) | 2019-07-19 | 2019-07-19 | 細胞の分化状態の評価方法 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2021014515A1 JPWO2021014515A1 (https=) | 2021-01-28 |
| JPWO2021014515A5 true JPWO2021014515A5 (https=) | 2022-03-17 |
| JP7344421B2 JP7344421B2 (ja) | 2023-09-14 |
Family
ID=74193489
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2021534873A Active JP7344421B2 (ja) | 2019-07-19 | 2019-07-19 | 細胞の分化状態の評価方法 |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20220260580A1 (https=) |
| EP (1) | EP4001425A4 (https=) |
| JP (1) | JP7344421B2 (https=) |
| CN (1) | CN114450418A (https=) |
| TW (1) | TWI855102B (https=) |
| WO (1) | WO2021014515A1 (https=) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114846151A (zh) * | 2020-01-17 | 2022-08-02 | 株式会社日立高新技术 | 细胞分化状态的评价方法以及细胞培养系统 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8048999B2 (en) | 2005-12-13 | 2011-11-01 | Kyoto University | Nuclear reprogramming factor |
| DK2209888T3 (da) * | 2007-10-12 | 2020-01-20 | Astellas Inst For Regenerative Medicine | Forbedrede fremgangsmåder til fremstilling af rpe-celler og sammensætninger af rpe-celler |
| WO2012056997A1 (ja) * | 2010-10-28 | 2012-05-03 | 国立大学法人熊本大学 | 多能性幹細胞の分化誘導効率を改善するための方法及び培地 |
| KR101357851B1 (ko) * | 2011-11-02 | 2014-02-06 | 제일약품주식회사 | 낭성구조물로부터 망막색소상피세포의 분화를 유도하는 방법 |
| US10519422B2 (en) * | 2012-02-29 | 2019-12-31 | Riken | Method of producing human retinal pigment epithelial cells |
| KR102254082B1 (ko) | 2012-08-24 | 2021-05-18 | 고쿠리쓰 겐큐 가이하쓰 호징 리가가쿠 겐큐소 | 망막 색소 상피 세포 시트의 제조 방법 |
| MX2016007671A (es) * | 2013-12-11 | 2016-10-14 | Pfizer Ltd | Metodo para producir celulas del epitelio pigmentario retiniano. |
| US11022600B2 (en) * | 2014-05-01 | 2021-06-01 | Shimadzu Corporation | Method for assessing state of differentiation of cells |
| SG11201801770VA (en) * | 2015-09-08 | 2018-04-27 | Sumitomo Dainippon Pharma Co Ltd | Method for producing retinal pigment epithelial cells |
| CN106609256B (zh) * | 2015-10-22 | 2020-04-07 | 同济大学 | 体外诱导人胚胎干细胞分化为视网膜色素上皮细胞的方法 |
| WO2017068727A1 (ja) * | 2015-10-23 | 2017-04-27 | 株式会社島津製作所 | 細胞の分化状態の評価方法 |
-
2019
- 2019-07-19 WO PCT/JP2019/028556 patent/WO2021014515A1/ja not_active Ceased
- 2019-07-19 US US17/628,023 patent/US20220260580A1/en active Pending
- 2019-07-19 JP JP2021534873A patent/JP7344421B2/ja active Active
- 2019-07-19 CN CN201980098491.9A patent/CN114450418A/zh active Pending
- 2019-07-19 EP EP19938680.6A patent/EP4001425A4/en active Pending
-
2020
- 2020-06-29 TW TW109121789A patent/TWI855102B/zh active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Arouri et al. | Corporate social responsibility and M&A uncertainty | |
| Gouw et al. | Quantitative proteomics by metabolic labeling of model organisms | |
| Wyatt | Margalef's mandala and phytoplankton bloom strategies | |
| Kabukçuoğlu et al. | Inflation as a global phenomenon—Some implications for inflation modeling and forecasting | |
| Melonek et al. | Multifunctionality of plastid nucleoids as revealed by proteome analyses | |
| Nelson et al. | Quantitative analysis of protein turnover in plants | |
| WO2021183917A8 (en) | Systems and methods for deconvolution of expression data | |
| Zhang et al. | Natural succession on abandoned cropland effectively decreases the soil erodibility and improves the fungal diversity | |
| Goffaux et al. | Improving continuous–discrete interval observers with application to microalgae-based bioprocesses | |
| Muklason et al. | Fairness in examination timetabling: Student preferences and extended formulations | |
| BRPI0818507B8 (pt) | Método de otimização de uma sequência nucleotídica para expressão em um organismo alvo | |
| WO2006091918A3 (en) | Process, composition and kit for providing a stable whole blood calibrator/control | |
| RU2018104839A (ru) | Модифицированные нуклеозиды, нуклеотиды и нуклеиновые кислоты и их применение | |
| JP2018516077A5 (https=) | ||
| Xu et al. | Proteomics analysis reveals distinct involvement of embryo and endosperm proteins during seed germination in dormant and non-dormant rice seeds | |
| JPWO2021014515A5 (https=) | ||
| Tuckwell | Quantitative aspects of L-type Ca2+ currents | |
| Dierckx et al. | A new estimation method for Weibull-type tails based on the mean excess function | |
| Zhao et al. | High temperatures and urban entrepreneurship levels: Evidence from China | |
| Chun-ming et al. | Dynamic change of evapotranspiration and influenced factors in the spring maize field in Northeast China | |
| Fernández et al. | Nothofagus pumilio forest affected by recent tephra deposition in northern Patagonia. II-Shifts in diversity and structure of rhizosphere fungal communities | |
| Imai et al. | On finite truncation of infinite shot noise series representation of tempered stable laws | |
| Takashina et al. | A geometric approach to scaling individual distributions to macroecological patterns | |
| Barkan et al. | Multiple phase transitions shape biodiversity of a migrating population | |
| WO2018117773A3 (ko) | 변형된 당 대사 경로를 갖는 재조합 균주 및 이를 이용한 당이성화 효소의 스크리닝 방법 |