CN114404388B - 一种细胞外囊泡体外装载mRNA的方法 - Google Patents
一种细胞外囊泡体外装载mRNA的方法 Download PDFInfo
- Publication number
- CN114404388B CN114404388B CN202210329162.4A CN202210329162A CN114404388B CN 114404388 B CN114404388 B CN 114404388B CN 202210329162 A CN202210329162 A CN 202210329162A CN 114404388 B CN114404388 B CN 114404388B
- Authority
- CN
- China
- Prior art keywords
- mrna
- extracellular vesicles
- loading
- vitro
- extracellular
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
- A61K9/50—Microcapsules having a gas, liquid or semi-solid filling; Solid microparticles or pellets surrounded by a distinct coating layer, e.g. coated microspheres, coated drug crystals
- A61K9/5005—Wall or coating material
- A61K9/5063—Compounds of unknown constitution, e.g. material from plants or animals
- A61K9/5068—Cell membranes or bacterial membranes enclosing drugs
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/7088—Compounds having three or more nucleosides or nucleotides
- A61K31/7105—Natural ribonucleic acids, i.e. containing only riboses attached to adenine, guanine, cytosine or uracil and having 3'-5' phosphodiester links
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/88—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation using microencapsulation, e.g. using amphiphile liposome vesicle
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Pharmacology & Pharmacy (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Organic Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Virology (AREA)
- Botany (AREA)
- Medicinal Preparation (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
Description
Claims (8)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210329162.4A CN114404388B (zh) | 2022-03-31 | 2022-03-31 | 一种细胞外囊泡体外装载mRNA的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210329162.4A CN114404388B (zh) | 2022-03-31 | 2022-03-31 | 一种细胞外囊泡体外装载mRNA的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN114404388A CN114404388A (zh) | 2022-04-29 |
CN114404388B true CN114404388B (zh) | 2022-07-26 |
Family
ID=81263101
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210329162.4A Active CN114404388B (zh) | 2022-03-31 | 2022-03-31 | 一种细胞外囊泡体外装载mRNA的方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN114404388B (zh) |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108904468A (zh) * | 2017-08-31 | 2018-11-30 | 李牧青 | 一种构建靶向细胞外囊泡的方法 |
CN110548007B (zh) * | 2018-05-31 | 2023-03-28 | 北京泰德制药股份有限公司 | 阳离子脂质体、基于其的核酸类药物递送系统及其制备方法与用途 |
CN110714015B (zh) * | 2019-10-29 | 2021-03-16 | 珠海丽凡达生物技术有限公司 | 一种mRNA狂犬病疫苗 |
CN110638759A (zh) * | 2019-10-29 | 2020-01-03 | 珠海丽凡达生物技术有限公司 | 一种用于体外转染和体内递送mRNA的制剂 |
CN113292608B (zh) * | 2021-07-27 | 2021-11-12 | 天九再生医学(天津)科技有限公司 | 一种外泌体药物递送系统及其制备方法和应用 |
CN114081943B (zh) * | 2021-11-08 | 2024-04-02 | 中国医学科学院医学生物学研究所 | 一种水痘-带状疱疹mRNA疫苗组合物及其制备方法和应用 |
CN114099533A (zh) * | 2021-11-25 | 2022-03-01 | 中国药科大学 | 核酸药物递送系统、制备方法、药物组合物和应用 |
-
2022
- 2022-03-31 CN CN202210329162.4A patent/CN114404388B/zh active Active
Also Published As
Publication number | Publication date |
---|---|
CN114404388A (zh) | 2022-04-29 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
AU2021200486B2 (en) | Compositions comprising synthetic polynucleotides encoding CRISPR related proteins and synthetic sgRNAs and methods of use | |
Avci-Adali et al. | Optimized conditions for successful transfection of human endothelial cells with in vitro synthesized and modified mRNA for induction of protein expression | |
AU2023201630A1 (en) | Circular RNA for translation in eukaryotic cells | |
JP4339852B2 (ja) | 遺伝子サイレンシングに関する方法および組成物 | |
CN105567641B (zh) | 一种携带抗肿瘤蛋白靶向性exosome的制备方法及其应用 | |
CN116323945A (zh) | 驱动环状rna翻译的遗传元件和使用方法 | |
CN108929874B (zh) | 一种特异性结合高表达pdl1蛋白的细胞的核酸适配体及其应用 | |
JP2022517275A (ja) | Trem組成物及びその使用 | |
CN105985978B (zh) | 一种新型rna环化表达载体的构建及其应用 | |
WO2023213237A1 (zh) | 一种含开环核苷结构的起始加帽寡核苷酸引物 | |
CN105671082B (zh) | 一种表达外泌体标记物的慢病毒载体及其构建方法和应用 | |
KR20220126725A (ko) | 유전자 편집을 위한 변형된 가이드 rna | |
JP2022523117A (ja) | ボルテックスミキサならびにその関連する方法、システム、及び装置 | |
US20080153763A1 (en) | Efficient Process For Producing Dumbbell Dna | |
CN115404240A (zh) | 制备环形rna的构建体、方法及其用途 | |
CN113423418A (zh) | 纤毛疾病的治疗 | |
CN116113697A (zh) | 用于治疗癫痫的方法和组合物 | |
WO2018184267A1 (zh) | mRNA编码的纳米抗体及其应用 | |
CN114404388B (zh) | 一种细胞外囊泡体外装载mRNA的方法 | |
CN111088323A (zh) | 筛选跨膜靶蛋白适配体的细胞selex方法及跨膜靶蛋白适配体及其应用 | |
CN115927480A (zh) | 一种基于核酸纳米结构的基因递送系统及其制备方法和应用 | |
CN111424017B (zh) | 一种装载shRNA的外泌体及其构建方法与应用 | |
Sawai et al. | A novel method of cell-specific mRNA transfection | |
CN114107306B (zh) | Cas9蛋白的核酸适配体及其应用 | |
JP2023507181A (ja) | ポリヌクレオチドをエクソソームに送達するための核酸コンストラクト |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Ge Xiaohu Inventor after: Lu Lu Inventor after: Zhang Quan Inventor after: Chai Tiancong Inventor after: Tian Yingzhou Inventor after: Wang Da Inventor after: Yin Jianxin Inventor after: Wang Miao Inventor after: Han Chunle Inventor before: Ge Xiaohu Inventor before: Chai Tiancong Inventor before: Lu Lu Inventor before: Zhang Quan Inventor before: Tian Yingzhou Inventor before: Wang Da Inventor before: Yin Jianxin Inventor before: Wang Miao Inventor before: Han Chunle |
|
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20220629 Address after: 300457 906, building 22, Green Valley Health Industrial Park, No. 59, Kangtai Avenue, Binhai Science Park, Binhai New Area, Tianjin Applicant after: Tianjin exosome Technology Co.,Ltd. Address before: 300457 No. 59, Kangtai Avenue, Binhai Science Park, Binhai New Area, Tianjin Applicant before: Tianjiu regenerative medicine (Tianjin) Technology Co.,Ltd. |
|
GR01 | Patent grant | ||
GR01 | Patent grant |