CN114395584B - 表达方法 - Google Patents

表达方法 Download PDF

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Publication number
CN114395584B
CN114395584B CN202111508425.XA CN202111508425A CN114395584B CN 114395584 B CN114395584 B CN 114395584B CN 202111508425 A CN202111508425 A CN 202111508425A CN 114395584 B CN114395584 B CN 114395584B
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CN
China
Prior art keywords
expression
polypeptide
host cell
target
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202111508425.XA
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English (en)
Chinese (zh)
Other versions
CN114395584A (zh
Inventor
F·L·桑德斯
A·L·多兹
A·M·G·贝亚德
B·V·卡拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Fujifilm Diosynth Biotechnologies UK Ltd
Original Assignee
Fujifilm Diosynth Biotechnologies UK Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GB201308017A external-priority patent/GB201308017D0/en
Priority claimed from GB201320339A external-priority patent/GB201320339D0/en
Application filed by Fujifilm Diosynth Biotechnologies UK Ltd filed Critical Fujifilm Diosynth Biotechnologies UK Ltd
Publication of CN114395584A publication Critical patent/CN114395584A/zh
Application granted granted Critical
Publication of CN114395584B publication Critical patent/CN114395584B/zh
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/30—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells
    • C07K16/3076—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties
    • C07K16/3092—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants from tumour cells against structure-related tumour-associated moieties against tumour-associated mucins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
CN202111508425.XA 2013-05-03 2014-04-29 表达方法 Active CN114395584B (zh)

Applications Claiming Priority (6)

Application Number Priority Date Filing Date Title
GB1308017.1 2013-05-03
GB201308017A GB201308017D0 (en) 2013-05-03 2013-05-03 Expression process
GB1320339.3 2013-11-18
GB201320339A GB201320339D0 (en) 2013-11-18 2013-11-18 Expression Process
CN201480024993.4A CN105392889A (zh) 2013-05-03 2014-04-29 表达方法
PCT/GB2014/000165 WO2014177826A1 (en) 2013-05-03 2014-04-29 Expression process

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201480024993.4A Division CN105392889A (zh) 2013-05-03 2014-04-29 表达方法

Publications (2)

Publication Number Publication Date
CN114395584A CN114395584A (zh) 2022-04-26
CN114395584B true CN114395584B (zh) 2025-01-28

Family

ID=50639794

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202111508425.XA Active CN114395584B (zh) 2013-05-03 2014-04-29 表达方法
CN201480024993.4A Pending CN105392889A (zh) 2013-05-03 2014-04-29 表达方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201480024993.4A Pending CN105392889A (zh) 2013-05-03 2014-04-29 表达方法

Country Status (12)

Country Link
US (3) US9873891B2 (https=)
EP (1) EP2992104B1 (https=)
JP (1) JP6545153B2 (https=)
KR (1) KR102180660B1 (https=)
CN (2) CN114395584B (https=)
BR (1) BR112015027373A2 (https=)
CA (1) CA2910877C (https=)
DK (1) DK2992104T3 (https=)
ES (1) ES2732907T3 (https=)
RU (1) RU2731717C2 (https=)
SG (1) SG11201508447TA (https=)
WO (1) WO2014177826A1 (https=)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3323826A1 (en) * 2016-11-21 2018-05-23 Danmarks Tekniske Universitet Native chinese hamster ovary cell secretion signal peptides for production of recombinant polypeptides
CN111465686B (zh) 2017-12-11 2024-03-22 富士胶片株式会社 动物细胞、动物细胞的制造方法及靶蛋白的制造方法
WO2020122049A1 (ja) 2018-12-11 2020-06-18 富士フイルム株式会社 動物細胞、動物細胞の製造方法および目的タンパク質の製造方法
CN109837277A (zh) * 2019-03-01 2019-06-04 深圳生生凡非基因技术有限公司 一种cpgk嵌合型启动子、腺相关病毒载体及其应用
WO2021107123A1 (ja) 2019-11-29 2021-06-03 富士フイルム株式会社 細胞培養方法、抗体製造方法、有機酸除去方法、及び、抗体
WO2022149607A1 (ja) 2021-01-07 2022-07-14 富士フイルム株式会社 細胞の選択方法、装置、及び装置システム
WO2022196710A1 (ja) 2021-03-17 2022-09-22 富士フイルム株式会社 細胞培養方法、及び有用物質の製造方法
CN112980852B (zh) * 2021-05-18 2021-08-13 北京华芢生物技术有限公司 新型冠状病毒b.1.351南非突变株rbd的基因及其应用
US20230011134A1 (en) 2021-05-27 2023-01-12 Janssen Biotech, Inc. Compositions and methods for the treatment of prostate cancer
EP4410957A4 (en) 2021-09-30 2026-01-28 Fujifilm Corp CELL CULTURE PROCESS AND PROCESS FOR PRODUCING USEFUL SUBSTANCE
EP4450609A1 (en) 2021-12-15 2024-10-23 FUJIFILM Corporation Method for producing product, and cell culture apparatus
CN118974267A (zh) 2022-03-28 2024-11-15 富士胶片株式会社 产物的生产方法及产物
JPWO2023190829A1 (https=) 2022-03-31 2023-10-05
JPWO2023238949A1 (https=) 2022-06-10 2023-12-14
EP4596704A4 (en) 2022-09-29 2026-03-11 Fujifilm Corp PRODUCT PRODUCTION PROCESS
WO2024248118A1 (ja) 2023-05-31 2024-12-05 富士フイルム株式会社 細胞培養方法および生産物の製造方法
CN121175432A (zh) 2023-05-31 2025-12-19 富士胶片株式会社 生产物的生产方法
EP4722377A1 (en) 2023-05-31 2026-04-08 FUJIFILM Corporation Method for producing product
WO2025244971A1 (en) 2024-05-20 2025-11-27 Janssen Biotech, Inc. Compositions and methods for the treatment of prostate cancer

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093295A2 (en) * 2002-04-30 2003-11-13 University Of North Carolina At Chapel Hill Secretion signal vectors
WO2012173344A2 (ko) * 2011-06-13 2012-12-20 주식회사종근당 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7186699B2 (en) * 2003-06-03 2007-03-06 Cell Genesys, Inc. Method for treating cancer by vector-mediated delivery of one or more anti-angiogenic or pro-apoptotic genes
US8029783B2 (en) * 2005-02-02 2011-10-04 Genentech, Inc. DR5 antibodies and articles of manufacture containing same
EP1739179A1 (en) * 2005-06-30 2007-01-03 Octapharma AG Serum-free stable transfection and production of recombinant human proteins in human cell lines
GB0602173D0 (en) * 2006-02-03 2006-03-15 Avecia Ltd Expression system
MX2009007139A (es) * 2006-12-28 2009-10-08 Centocor Ortho Biotech Inc Metodos y vectores para generar inmunoglobulinas no sialiladas.
KR101618767B1 (ko) * 2008-09-02 2016-05-09 한국생명공학연구원 외래단백질을 고효율로 생산하는 리더서열
CA2746350C (en) * 2008-12-31 2018-06-12 Bharat Serums And Vaccines Ltd. Anti-rhd monoclonal antibodies
TWI541021B (zh) 2009-03-05 2016-07-11 艾伯維有限公司 Ｉｌ－１７結合蛋白
GB0905331D0 (en) * 2009-03-27 2009-05-13 Avecia Biolog Ltd Expression process

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003093295A2 (en) * 2002-04-30 2003-11-13 University Of North Carolina At Chapel Hill Secretion signal vectors
WO2012173344A2 (ko) * 2011-06-13 2012-12-20 주식회사종근당 Ｃｓｐ－ｂ ５'-ｓａｒ 인자를 포함하는 동물세포 발현 벡터 및 이를 이용한 재조합 단백질의 제조방법

Also Published As

Publication number Publication date
US20180127777A1 (en) 2018-05-10
CA2910877A1 (en) 2014-11-06
US20220213500A1 (en) 2022-07-07
DK2992104T3 (da) 2019-07-15
CA2910877C (en) 2021-08-03
RU2015151626A3 (https=) 2018-03-20
EP2992104A1 (en) 2016-03-09
JP6545153B2 (ja) 2019-07-17
SG11201508447TA (en) 2015-11-27
CN114395584A (zh) 2022-04-26
EP2992104B1 (en) 2019-04-17
KR20160003705A (ko) 2016-01-11
BR112015027373A2 (pt) 2017-08-29
US11306323B2 (en) 2022-04-19
JP2016517691A (ja) 2016-06-20
US9873891B2 (en) 2018-01-23
RU2731717C2 (ru) 2020-09-08
WO2014177826A1 (en) 2014-11-06
ES2732907T3 (es) 2019-11-26
US20160076051A1 (en) 2016-03-17
KR102180660B1 (ko) 2020-11-19
CN105392889A (zh) 2016-03-09
RU2015151626A (ru) 2017-06-08

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