CN114250122B - 自悬浮生物酶长效绿色洗涤剂 - Google Patents

自悬浮生物酶长效绿色洗涤剂 Download PDF

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CN114250122B
CN114250122B CN202111648733.2A CN202111648733A CN114250122B CN 114250122 B CN114250122 B CN 114250122B CN 202111648733 A CN202111648733 A CN 202111648733A CN 114250122 B CN114250122 B CN 114250122B
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biological enzyme
self
detergent
suspending
long
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CN114250122A (zh
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庄巧妮
余春柳
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Hengan Fujian Holding Group Co Ltd
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Hengan Fujian Holding Group Co Ltd
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Abstract

本发明公开一种自悬浮生物酶长效绿色洗涤剂，所述的洗涤剂由生物酶凝胶制成，所述的生物酶为蛋白酶、淀粉酶、纤维素酶、脂肪酶、甘露聚糖酶、果胶酶中的两种以上。本发明通过提高生物酶的使用比例达到清洁效果，并将复配生物酶包裹形成稳定生物酶凝胶，可以解决酶制剂在洗涤剂中应用受限；其次，α‑CD接枝改性细菌纤维素包裹生物酶，形成自悬浮生物酶水凝胶，方便在洗涤剂中的应用；再次，细菌纤维素具有极细的纤维直径(80‑100nm),悬浮力强，透明度高；整个生物酶的包裹工艺反应条件温和简单，反应时间短，反应成分绿色安全，包裹率高达80％以上。

Description

自悬浮生物酶长效绿色洗涤剂
技术领域
本发明涉及一种添加生物酶的绿色洗涤剂领域，具体涉及一种自悬浮生物酶长效绿色洗涤剂。
背景技术
现有传统洗涤剂依靠表面活性剂去污，当表面活性剂的活性浓度达到一定时，去污力也随之达到瓶颈效果。酶制剂分子通过在空间中形成专一的立体3D 结构发挥作用精准剪切污渍，可以减少表面活性剂的用量，进一步的提升洗涤效果，为新一代的去污成分。但是酶制剂存在易受表面活性剂、pH、自由水、螯合剂和温度等众多影响而失去活性。如何让生物酶在洗涤剂中长久、稳定的发挥功效成了行业难点。
发明内容
为克服上述缺陷，本发明的目的在于提供一种自悬浮生物酶长效绿色洗涤剂
为达到上述目的，本发明的自悬浮生物酶长效绿色洗涤剂，所述的洗涤剂由生物酶凝胶制成，所述的生物酶为蛋白酶、淀粉酶、纤维素酶、脂肪酶、甘露聚糖酶、果胶酶中的两种以上。
进一步的，所述的生物酶为：蛋白酶、淀粉酶和纤维素酶；其中，各生物酶的质量百分比为：蛋白酶：淀粉酶：纤维素酶＝2-5％：0.5-2.5％：1.8-3.8％。
进一步的，所述的生物酶为：淀粉酶、脂肪酶和果胶酶；其中，各生物酶质量百分比为：淀粉酶：脂肪酶：果胶酶＝2.5-3.5％：1.2-3.2％：0.8-2.8％。
进一步的，所述的蛋白酶为丝氨酸内切蛋白酶的protease unit PRMU-U，至少为100PRMU-U/g；所述的纤维素酶的CELLULASE unit CNU-R至少为 4500CNU-R/g；
所述的脂肪酶为lipase LCLU-SL至少为100LCLU-SL/g；
内切-1,4-β-甘露聚糖酶mannanase unit MIUM至少为8MIUN/g；
果胶酯酶的pectin degrading enzyme PDEU至少为1000PDEU/g。
进一步的，所述的生物酶凝胶是按下述步骤制备的：
1)将1份α-环糊精(α-CD)置于1-1.5份90℃丁烷四羧酸(BTCA)水溶液中溶解，加入催化剂0.3-0.6份次亚磷酸钠(SHP)，预交联完全后，加入细菌纤维素搅拌扩散处理1h；
2)利用0.2-0.5wt％辣根过氧化氢酶催化0.1-0.3wt％2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)生成正离子自由基ABTS●+，引发巯基-烯点击化学反应，令20-30wt％四臂聚乙二醇丙烯酸酯(4arm-PEG-AA)和15-25wt％四臂巯基聚乙二醇两种PEG衍生物与45-55wt％α-CD接枝细菌纤维素发生自组装，同时将上述两中以上的生物酶包裹在PRX水凝胶中形成自悬浮生物酶水凝胶。
进一步的，所述的洗涤剂添加有烷基糖苷(C8-C10、C12-C14)、脂肪醇聚氧乙烯醚羧酸钠、醇醚羧酸钠、脂肪醇聚氧乙烯醚、椰油酰胺丙基甜菜碱中的两种或多种。
进一步的，所述的洗涤剂添加有谷氨酸二乙酸四钠(GLDA)、DTMPA、柠檬酸钠中的一种。
进一步的，所述的洗涤剂添加有过碳酸钠、过氧化氢、聚丙烯酸钠的随意一种或多种。
进一步的，所述的洗涤剂添加有卡松、BIOBANTM551S(陶氏化学)中的一种或两种。
进一步的，所述的洗涤剂由下述质量份的原料制成：水50-85份、脂肪醇聚氧乙烯醚羧酸钠5-10份、醇醚羧酸钠5-15、椰油酰胺丙基甜菜碱3-10、谷氨酸二乙酸四钠0.1-0.5份、细菌纤维素1-5份、生物酶凝胶3-15份、卡松0.2-1份。
本发明通过提高生物酶的使用比例达到清洁效果，并将复配生物酶包裹形成稳定生物酶凝胶，可以解决酶制剂在洗涤剂中应用受限；其次，α-CD接枝改性细菌纤维素包裹生物酶，形成自悬浮生物酶水凝胶，方便在洗涤剂中的应用；再次，细菌纤维素具有极细的纤维直径(80-100nm),悬浮力强，透明度高；更佳的，生物酶的包裹工艺反应条件温和简单，反应时间短，反应成分绿色安全，包裹率高达80％以上。
具体实施方式
下面结合实施例对本发明作进一步的说明。
本发明添加生物酶包括蛋白酶、淀粉酶、纤维素酶、脂肪酶、甘露聚糖酶、果胶酶。.选用的蛋白酶为丝氨酸内切蛋白酶的protease unit PRMU-U至少为 100PRMU-U/g；纤维素酶的CELLULASE unit CNU-R至少为4500CNU-R/g；脂肪酶为lipase LCLU-SL至少为100LCLU-SL/g；内切-1,4-β-甘露聚糖酶mannanase unit MIUM至少为8MIUN/g；果胶酯酶的pectin degrading enzyme PDEU至少为 1000PDEU/g。
生物酶复合物可以根据需求，由一种或多种自由组合，具体推荐方案1(建议添加至衣料用洗涤剂)：蛋白酶：淀粉酶：纤维素酶＝2-5％：0.5-2.5％：1.8-3.8％；具体推荐方案2(建议添加至餐具净)：淀粉酶：脂肪酶：果胶酶＝2.5-3.5％： 1.2-3.2％：0.8-2.8％。
本发明添加表面活性剂包括烷基糖苷(C8-C10、C12-C14)、脂肪醇聚氧乙烯醚羧酸钠、醇醚羧酸钠、脂肪醇聚氧乙烯醚、椰油酰胺丙基甜菜碱中的两种或多种；
本发明添加螯合剂包括谷氨酸二乙酸四钠(GLDA)、DTMPA、柠檬酸钠的随意一种；
本发明添加洗涤助剂包括过碳酸钠、过氧化氢、聚丙烯酸钠的随意一种或多种；
本发明添加防腐剂包括卡松、BIOBANTM551S(陶氏化学)中的随意一种或两种。
洗涤剂的推荐方案：水50-85份、脂肪醇聚氧乙烯醚羧酸钠5-10份、醇醚羧酸钠5-15、椰油酰胺丙基甜菜碱3-10、谷氨酸二乙酸四钠0.1-0.5份、细菌纤维素1-5份、生物酶凝胶3-15份、卡松0.2-1份。
洗涤剂的工艺：先将水凝胶生物酶悬浮剂与3倍量的水4000r/min均质完全做悬浮液备用，再将剩余水加入烧杯，升温至60-70℃，依次溶解表面活性剂、螯合剂，降至室温加入防腐剂和悬浮液至均相。
上述的生物酶凝胶的制备过程包括：
生物酶的包裹方案：
α-CD接枝细菌纤维素：将1份α-环糊精(α-CD)置于1-1.5份90℃丁烷四羧酸(BTCA)水溶液中溶解，加入催化剂0.3-0.6份次亚磷酸钠(SHP)，预交联完全后，加入细菌纤维素搅拌扩散处理1h。
工艺：利用0.2-0.5wt％辣根过氧化氢酶催化0.1-0.3wt％2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)生成正离子自由基ABTS●+，引发巯基-烯点击化学反应，令20-30wt％四臂聚乙二醇丙烯酸酯(4arm-PEG-AA)和15-25wt％四臂巯基聚乙二醇两种PEG衍生物与45-55wt％α-CD接枝细菌纤维素发生自组装，同时将上述配方酶包裹在PRX水凝胶中形成自悬浮生物酶水凝胶。
其中，四臂聚乙二醇丙烯酸酯(4arm-PEG-AA)：99％纯度，20000分子量，厦门赛诺邦格生物科技股份有限公司；四臂聚乙二醇硫醇(4arm-PEG-SH)：99％纯度，20000分子量，厦门赛诺邦格生物科技股份有限公司；α-环糊精(α-CD)： 99％上海阿拉丁生化科技股份有限公司；辣根过氧化物酶(horse radish peroxidase，HRP)：300U/mg生工生物工程(上海)股份有限公司；细菌纤维素：CPKelco公司。

Claims (9)

1.一种自悬浮生物酶长效绿色洗涤剂，其特征在于，所述的洗涤剂由生物酶凝胶制成，所述的生物酶为蛋白酶、淀粉酶、纤维素酶、脂肪酶、甘露聚糖酶、果胶酶中的两种以上；
所述的生物酶凝胶是按下述步骤制备的：
1)将1份α-环糊精(α-CD)置于1-1.5份90℃丁烷四羧酸(BTCA)水溶液中溶解，加入催化剂0.3-0.6份次亚磷酸钠(SHP)，预交联完全后，加入细菌纤维素搅拌扩散处理1h；
2)利用0.2-0.5wt％辣根过氧化氢酶催化0.1-0.3wt％2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)生成正离子自由基ABTS●+，引发巯基-烯点击化学反应，令20-30wt％四臂聚乙二醇丙烯酸酯(4arm-PEG-AA)和15-25wt％四臂巯基聚乙二醇两种PEG衍生物与45-55wt％α-CD接枝细菌纤维素发生自组装，同时将上述两中以上的生物酶包裹在PRX水凝胶中形成自悬浮生物酶水凝胶。
2.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的生物酶为：蛋白酶、淀粉酶和纤维素酶；其中，各生物酶的质量百分比为：蛋白酶：淀粉酶：纤维素酶＝2-5％：0.5-2.5％：1.8-3.8％。
3.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的生物酶为：淀粉酶、脂肪酶和果胶酶；其中，各生物酶质量百分比为：淀粉酶：脂肪酶：果胶酶＝2.5-3.5％：1.2-3.2％：0.8-2.8％。
4.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，其特征在于，所述的蛋白酶为丝氨酸内切蛋白酶的protease unit PRMU-U，至少为100PRMU-U/g；所述的纤维素酶的CELLULASE unit CNU-R至少为4500CNU-R/g；
所述的脂肪酶为lipase LCLU-SL至少为100LCLU-SL/g；
内切-1,4-β-甘露聚糖酶mannanase unit MIUM至少为8MIUN/g；
果胶酯酶的pectin degrading enzyme PDEU至少为1000PDEU/g。
5.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的洗涤剂添加有烷基糖苷(C8-C10、C12-C14)、脂肪醇聚氧乙烯醚羧酸钠、醇醚羧酸钠、脂肪醇聚氧乙烯醚、椰油酰胺丙基甜菜碱中的两种或多种。
6.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的洗涤剂添加有谷氨酸二乙酸四钠(GLDA)、DTMPA、柠檬酸钠中的一种。
7.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的洗涤剂添加有过碳酸钠、过氧化氢、聚丙烯酸钠的随意一种或多种。
8.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的洗涤剂添加有卡松、BIOBANTM551S(陶氏化学)中的一种或两种。
9.如权利要求1所述的自悬浮生物酶长效绿色洗涤剂，，其特征在于，所述的洗涤剂由下述质量份的原料制成：水50-85份、脂肪醇聚氧乙烯醚羧酸钠5-10份、醇醚羧酸钠5-15、椰油酰胺丙基甜菜碱3-10、谷氨酸二乙酸四钠0.1-0.5份、细菌纤维素1-5份、生物酶凝胶3-15份、卡松0.2-1份。
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