CN114209748B

CN114209748B - Traditional Chinese medicine composition for preventing African swine fever and application and preparation thereof

Info

Publication number: CN114209748B
Application number: CN202111583950.8A
Authority: CN
Inventors: 贾梧松; 王振; 杜贺平; 贾卫东; 秦军波
Original assignee: Henan Gede Animal Pharmaceutical Co ltd; Henan Traditional Chinese Veterinary Drug Research Institute Co ltd
Current assignee: Henan Gede Animal Pharmaceutical Co ltd; Henan Traditional Chinese Veterinary Drug Research Institute Co ltd
Priority date: 2021-12-22
Filing date: 2021-12-22
Publication date: 2023-04-25
Anticipated expiration: 2041-12-22
Also published as: CN114209748A

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for preventing African swine fever, and application and a preparation thereof. The composition comprises radix astragali, radix Ginseng, rhizoma Atractylodis Macrocephalae preparata, rhizoma Atractylodis, pericarpium Citri Reticulatae, rhizoma Osmundae, radix Isatidis, radix Saposhnikoviae, and radix Glycyrrhizae. According to the traditional Chinese medicine compatibility theory, the raw materials are selected and scientifically and reasonably combined, and the obtained composition has a remarkable prevention effect on African swine fever. Experiments show that the effective rate of the composition for preventing African swine fever can reach more than 95%, and the composition is obviously superior to a missing formula control.

Description

Traditional Chinese medicine composition for preventing African swine fever and application and preparation thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition for preventing African swine fever, and application and a preparation thereof.

Background

African Swine Fever (ASF) (hereinafter referred to as "African fever") has been discovered for the first time in Dongfen Kenya in 1909, and has been a history of over 100 years. Have been popular in south africa, spanish, france, copa, russia, etc., and have resulted in significant economic losses in many countries. However, no one country has developed a safe and effective vaccine or drug for prevention, nor has no country developed a therapeutic drug with good effect.

After the outbreak of non-pestilence epidemic situation in 2018 and 8 days in 2019, the department of agriculture holds remote training for controlling African swine fever, and in the training conference, the group of national non-pestilence expert group companies, yang Hanchun professor of Chinese agricultural university, teaches: non-pestilence is an infectious disease with high mortality (up to 100%), and so far, there is no safe and effective vaccine for prevention, nor effective therapeutic drug.

Exploration in western veterinarian. The main steps are as follows: lauric acid monoglyceride, enrofloxacin, naissance to house-hold pigs, egg yolk antibodies, etc., but all have been practically proven ineffective. Particularly, the gene-deleted vaccine produced privately is used for preventing the non-plague, so that not only does not have a good prevention effect, but also the vaccine is harmful to the pig body, and more serious, the gene-deleted strain is caused to pollute the environment, and atypical non-plague is caused, so that the prevention and control of the non-plague are more complicated and difficult.

And (3) exploring the medical aspect of Chinese beasts. Since the outbreak of non-pestilence in China, many veterinary science and technology personnel know that western veterinary medicines have no good way to prevent and treat the disease, and breakthrough is made in the aspects of searching for and exploring Chinese veterinary medicines in a dispute, but a traditional Chinese medicine product with good effect is not found under the guidance of the pharmacological theory of the real Chinese veterinarian.

The traditional Chinese veterinary medicine is mainly used for preventing the following cases of non-pestilence:

(1) according to the guidance of western veterinary theory, several Chinese medicines with immunity enhancing effect are pieced together, and the Chinese medicines are sold and used in the market, and have poor effects although having some effects.

(2) The theory of "taking poison as poison" is that the purpose of improving the immunity of the organism is achieved by taking the toxic Chinese medicaments such as toads and the like by oral administration, and the practice proves that the Chinese medicaments are basically ineffective.

(3) The combination of the Chinese and the western medicines not only uses some traditional Chinese medicines for clearing heat and detoxicating, but also uses antipyretic analgesic chemicals and antibiotics in a matching way, and the practice proves that the traditional Chinese medicine can only relieve symptoms of the pigs suffering from the diseases, prevent secondary bacterial infection and is ineffective for preventing and treating the non-plague.

(4) Chinese medicine + probiotics, or fermented Chinese medicine. But the effect is not obvious by taking western veterinary theory as a guide.

(5) Some of the traditional Chinese medicines are used singly, and some of the traditional Chinese medicines with the effects of clearing heat and detoxicating are bitter and cold, so that the result effect is not good.

(6) Treatment of the illegal. The traditional Chinese medicine composition also obviously violates the national regulations of 'african swine fever not allowed to treat', and western medicines or traditional Chinese medicines are used for treatment without permission, so that the traditional Chinese medicine composition is not effective and can dispel toxin.

The above prevention and treatment methods have problems:

(1) instead of guiding the basic theory of traditional Chinese medicine, the traditional Chinese medicine is marked, and the traditional Chinese medicines are spliced under the guidance of western medicine theory, so that the basic theory principle of traditional Chinese medicine is completely violated.

(2) The combination of the Chinese and western medicines completely deviates from the basic theoretical guidance and practice of the principles, methods, prescriptions, medicines and the like of the Chinese and veterinary medicines, and is not a real Chinese medicine at all.

(3) The medicine is not distinguished. The treatment based on syndrome differentiation is one of the characteristics and advantages of middle veterinarian, and can be used for treating the disease obviously without syndrome differentiation, and can be used for only tonifying qi and strengthening healthy energy, so that strengthening healthy energy and retaining evil is generated; some medicines for clearing heat and removing toxicity are only used for eliminating pathogenic factors, so that the deficiency of bitter and cold can damage yang qi, damage spleen and stomach, and damage healthy energy due to pathogenic factors; the result is not only bad effect, but also damage and reduce disease resistance and immunity of organism.

(4) The traditional Chinese medicine and the fermented traditional Chinese medicine and probiotics are the medicines under the guidance of western medicine theory, do not accord with the basic theory of traditional Chinese medicine (veterinary) at all, and lack the guidance of dialectical treatment theory.

(5) No safe and effective non-pestilent vaccine is officially approved by the government in the world. The non-pestilence gene deletion vaccine used in illegal production has no preventive effect, seriously damages the health of pig groups, and more seriously spreads the gene deletion strain into the environment to cause the transmission of atypical non-pestilence. Currently, the agricultural rural department has determined it as illegal and is looking up and processing it all over the country.

The outbreak and the popularity of the non-pestilence have great influence on politics, economy and civilian life in China, and particularly bring great harm to the pig industry and peasant income increase. Up to now, western veterinarians have neither developed safe and effective vaccine prophylaxis nor effective chemical control.

Disclosure of Invention

In view of the above, the invention provides a traditional Chinese medicine composition for preventing African swine fever. The composition has remarkable prevention effect on African swine fever, and the prevention effective rate is more than 95%.

In order to achieve the above object, the present invention provides the following technical solutions:

a Chinese medicinal composition for preventing African swine fever comprises radix astragali, ginseng radix, rhizoma Atractylodis Macrocephalae preparata, rhizoma Atractylodis, pericarpium Citri Tangerinae, rhizoma Osmundae, radix Isatidis, radix Saposhnikoviae, and Glycyrrhrizae radix.

The pestilence is a category of the pestilence in African swine fever (veterinary) doctors. Pestilence is caused by pestilence toxin attacking the exterior and weak healthy qi. Therefore, there is a discussion of "pathogenic factors are all together and their qi is deficient" in Huang Di Nei Jing ".

The formula selects astragalus root, has the effects of nourishing middle-jiao and replenishing qi, strengthening exterior and resisting pathogenic factors, replenishing primordial qi, treating consumptive disease, wen Sanjiao, strengthening spleen and stomach, and is suitable for all symptoms of deficiency. In addition, ginseng and largehead atractylodes rhizome are used as ministerial drugs, and the ginseng is sweet, warm and nontoxic, can greatly tonify primordial qi, calm the spirit, remove pathogenic qi, and is good for tonifying qi, blood and body fluid; spleen and stomach are the acquired sources of qi and blood, so that bighead atractylodes rhizome has the effects of strengthening spleen and replenishing qi, strengthening spleen and stomach, eliminating diseases, strengthening spleen and stomach, and promoting diet, and has the effect of preventing miscarriage; the two ministerial drugs assist the monarch drugs, the biochemistry and the active drugs are the same as the medical drugs. Then, cyrtomium fortunei, radix isatidis, radix sileris, rhizoma atractylodis and the like are selected to clear plague and detoxify, so as to dispel pathogenic factors of the pestilence in the heaven, prevent epidemic disease and qi, and treat all pathogenic wind. It is combined with Glycyrrhrizae radix and pericarpium Citri Tangerinae to regulate the functions of the drugs to relieve various toxic substances, and can be used for supplementing without greasy, but not excessive. In a word, the medicine is combined to play the roles of strengthening body resistance and consolidating constitution, eliminating toxin, building up body, avoiding plague and expelling epidemic disease; the aims of strengthening the body resistance without retaining the evil, eliminating the evil without damaging the body resistance and keeping the body resistance and the qi inside, and the evil can not be dried are achieved.

In the traditional Chinese medicine formula, the traditional Chinese medicines are selected based on the standard stipulated in the second part of the Chinese animal pharmacopoeia of 2015 edition and the first part of the Chinese people's republic of China, the genuine and quality-multiplied genuine traditional Chinese medicines of the production area are selected, and the traditional Chinese medicines are processed carefully according to the result of treatment based on syndrome differentiation and the formula principles of monarch, minister, assistant and guide.

The composition provided by the invention is prepared from the following raw materials in parts by weight:

26-35 parts of astragalus membranaceus, 5-12 parts of ginseng, 5-15 parts of ground-fried bighead atractylodes rhizome, 4-12 parts of rhizoma atractylodis, 5-10 parts of dried orange peel, 5-14.5 parts of cyrtomium fortunei, 8-18 parts of radix isatidis, 4-12 parts of radix sileris and 5-10 parts of liquorice.

In the invention, the weight parts of the raw materials are preferably as follows: 28-33 parts of astragalus membranaceus, 7-10 parts of ginseng, 7-13 parts of ground-fried bighead atractylodes rhizome, 6-10 parts of rhizoma atractylodis, 6-10 parts of dried orange peel, 7-12.5 parts of cyrtomium fortunei, 11-15 parts of radix isatidis, 5-7.5 parts of radix sileris and 6-9 parts of liquorice.

In the invention, the composition consists of the following optimal components in parts by mass:

30 parts of astragalus membranaceus, 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 7.5 parts of rhizoma atractylodis, 8 parts of dried orange peel, 9.5 parts of cyrtomium fortunei, 13 parts of radix isatidis, 6 parts of radix sileris and 7.5 parts of liquorice.

In the invention, each raw material is a decoction piece obtained by processing raw materials of traditional Chinese medicines, wherein the decoction piece comprises a processed product.

The purpose of processing the traditional Chinese medicine is as follows: can remove impurities, change drug properties, improve therapeutic effect, and remove toxic and side effects. The Chinese medicinal materials selected in the formula are all strictly selected and processed according to the ancient way to be used as medicines, so that the quality is ensured, and the curative effect is improved. Such as: rhizoma Atractylodis Macrocephalae stir-fried with soil: parching the fine powder of Fulong liver in a pan, adding Atractylodis rhizoma tablet, parching to slight yellow, removing soil, sieving to remove soil, and cooling.

The invention also provides application of the traditional Chinese medicine composition in preparation of medicines for preventing African swine fever.

The invention also provides a medicine for preventing African swine fever, which comprises the traditional Chinese medicine composition and pharmaceutically acceptable auxiliary materials.

The medicament is in the form of powder and granules.

The preparation method of the powder comprises the following steps: mixing the Chinese medicinal decoction pieces, pulverizing with pulverizer, sieving with 120 mesh sieve, collecting, stirring, mixing, and packaging for storage.

The granule is prepared according to the requirements of two prescriptions of Chinese animal pharmacopoeia and the production process of the granule.

In some embodiments, the dosage form is granule, and the pharmaceutically acceptable auxiliary materials comprise glucose, starch and flavoring agent. The preparation method of the granule comprises the following steps:

soaking the Chinese medicinal decoction pieces, decocting, filtering, mixing filtrates, concentrating into fluid extract, adding anhydrous glucose, starch, and flavoring agent adjuvants, granulating, and oven drying;

the mass ratio of the total weight of the traditional Chinese medicine raw materials to the granules is 10:2.

the traditional Chinese medicine composition provided by the invention comprises astragalus, ginseng, earth-fried bighead atractylodes rhizome, rhizoma atractylodis, dried orange peel, cyrtomium rhizome, radix isatidis, radix sileris and liquorice. Experiments show that the effective rate of the traditional Chinese medicine composition for preventing African swine fever can reach more than 95%, and the traditional Chinese medicine composition is obviously superior to a missing formula control. Compared with the prior art, the invention has the following beneficial effects:

(1) the prevention is mainly performed. The concept of treating disease in the middle (veterinary) doctor is taken as guidance, and the outstanding prevention is mainly achieved. The "treating disease" in Huangdi's interior meridian has an urgent discussion: "the holy person is not treated with disease, not treated with disorder, this is also called as" the holy person is not treated with disorder ". The doctor's illness is finished and then the medicine is finished, and then the medicine is treated, for example, the doctor is thirsty and penetrates the well, the cone is cast, and the doctor is not late in-! This is an important component in the theory of Chinese (veterinary) medicine, and is also an important guiding idea for preventing and controlling non-plague and the like.

(2) Strengthening healthy qi and enhancing disease resistance and immunity of organisms. The "Huangdi's Nei Jing" states that: the healthy qi is stored in the body, and pathogenic factors cannot be dried. The pig body is infected and is not pestilent, and one important pig body has direct relation with the body's vital energy deficiency (the same is true of the new crown pneumonia). Therefore, it is important to strengthen the body's vital qi, raise the threshold (threshold) for infection such as non-pestilence and protect the susceptible swinery from or less from non-pestilence. Therefore, the purpose of the formula of the application is to select astragalus root, ginseng and the like.

(3) Anti-stress and immunity enhancing. Stress can significantly reduce the immune disease resistance of the body. We pay great attention to this problem, and have made arrangements in this formulation.

(4) Dispel pathogenic qi, dispel and kill toxic qi of epidemic pestilence. Pestilence pathogenic qi is an important external factor that causes non-pestilence. Preventing non-pestilence, strengthening healthy qi, and expelling and killing the toxin of pestilence. Only when the two pipes are aligned, the effect can be good. Therefore, we also pay attention to this in the formulation against non-plague. The Chinese medicinal components such as cyrtomium fortunei and the like for dispelling pathogenic factors and killing viruses are added into the formula, and the practice proves that the effect is good!

(5) Strengthen spleen and stomach, strengthen the acquired body, treat diseases and ask the root. Spleen and stomach are the acquired sources of qi and blood. After the patients get on the diet, the organism can be healthy and the disease resistance can be stronger. Therefore, the bighead atractylodes rhizome and the rhizoma atractylodis are added in the formula, are special for spleen and stomach, strengthen spleen and replenish stomach, are aromatic and can be used for avoiding dirty, treating pestilence in four times, preventing miscarriage and achieving multiple purposes.

(6) Regulating qi and removing toxic substances, and harmonizing various drugs. According to the conditions that the pig herd in China generally has weak healthy qi, persistent pathogenic qi and deficiency as the main factor, the strategy of strengthening healthy qi and eliminating pathogenic factors is adopted. However, in order to achieve the purposes of strengthening the body resistance without retaining the evil and eliminating the evil without damaging the body resistance, besides the design in terms of medicine dosage, the dried orange peel and the liquorice are additionally added to regulate the flow of qi and detoxify and harmonize the medicines, so that the purposes of eliminating the evil and restoring the body resistance are achieved.

(7) Convenient use, gas saving and labor saving. After the formula is processed, no matter the powder or the granule is adopted, the palatability is good, pigs are loved to eat, and the feed is mixed for feeding, so that the feed is convenient and quick, and the air and the labor are saved.

(8) The prevention effect is good. For more than two years, the method is popularized in farmers in provinces such as Henan, hebei, shanxi, guangzhou, fujian and the like, the general reflection effect is good, and if matched measures (comprehensive measures such as isolation, disinfection, feeding tube, conventional immunity, biosafety and the like) are added, the effective rate can be up to more than 95%. Thereby protecting thousands of pig farms from being harmed by non-plague, and being welcomed and favored by pig farmers.

Detailed Description

The invention provides a traditional Chinese medicine composition for preventing African swine fever, application and a preparation thereof. Those skilled in the art can, with the benefit of this disclosure, suitably modify the process parameters to achieve this. It is expressly noted that all such similar substitutions and modifications will be apparent to those skilled in the art, and are deemed to be included in the present invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those skilled in the relevant art that the invention can be practiced and practiced with modification and alteration and combination of the methods and applications herein without departing from the spirit and scope of the invention.

The traditional Chinese medicine raw materials adopted in the invention are all commercially available traditional Chinese medicine decoction pieces in the market.

The invention is further illustrated by the following efficacy experiments in conjunction with the examples:

efficacy experiment 1

The powder prepared by the traditional Chinese medicine composition comprises the following components: 30 parts of astragalus membranaceus, 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 7.5 parts of rhizoma atractylodis, 8 parts of dried orange peel, 9.5 parts of cyrtomium fortunei, 13 parts of radix isatidis, 6 parts of radix sileris and 7.5 parts of liquorice.

The preparation method comprises the following steps: mixing the Chinese medicinal decoction pieces, pulverizing with pulverizer, sieving with 120 mesh sieve, collecting, stirring, mixing, and packaging for storage.

Efficacy experimental case:

the number of live pigs in the stock of the Henan Luodan river is about 2500, the non-plague is generated in 4 months in 2019, the dead and panning live pigs are more than 200, and then under the guidance of a teacher in China, the powder prepared by adopting the composition of the invention is prepared by tooth pulling, disinfection and feeding, so that the outbreak epidemic of the non-plague is effectively prevented and controlled, most live pigs in the pig farm survive, and the cultivation benefit is always good.

1. The test method comprises the following steps:

(1) The 240 normal feeding nursery pigs were selected and randomly divided into six groups, which were respectively designated as experimental group, control group 1, control group 2, control group 3, control group 4 and control group 5. Each group is provided with 40 heads, two circles are divided, each circle is provided with 20 heads, and each group is hung with the same signboard.

(2) Grouping and medication conditions:

(1) experimental group (using the above full formula powder of the invention):

the formula comprises the following components: 30 parts of astragalus, 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 7.5 parts of rhizoma atractylodis, 9.5 parts of cyrtomium rhizome, 13 parts of radix isatidis, 6 parts of divaricate saposhnikovia root, 8 parts of dried orange peel and 7.5 parts of liquorice.

(2) Control 1 group (the invention is based on the whole formula without the monarch drug astragalus)

The formula comprises the following components: 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 7.5 parts of rhizoma atractylodis, 9.5 parts of cyrtomium rhizome, 13 parts of radix isatidis, 6 parts of divaricate saposhnikovia root, 8 parts of dried orange peel and 7.5 parts of liquorice.

(3) Control group 2 (the whole prescription of the invention is removed with 8.5 parts of ministerial ginseng and 10 parts of earth-fried white atractylodes rhizome)

The formula comprises the following components: 30 parts of astragalus, 7.5 parts of rhizoma atractylodis, 9.5 parts of cyrtomium rhizome, 13 parts of radix isatidis, 6 parts of radix sileris, 8 parts of dried orange peel and 7.5 parts of liquorice.

(4) Control group 3 (the invention is based on the whole formula, 7.5 parts of rhizoma atractylodis, 9.5 parts of cyrtomium fortunei, 13 parts of radix isatidis and 6 parts of radix sileris are removed).

The formula comprises the following components: 30 parts of astragalus, 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 8 parts of dried orange peel and 7.5 parts of liquorice.

(5) Control group 4 (8 parts of dried orange peel and 7.5 parts of liquorice are removed on the basis of the whole formula of the invention).

The formula comprises the following components: 30 parts of astragalus, 8.5 parts of ginseng, 10 parts of earth-fried bighead atractylodes rhizome, 7.5 parts of rhizoma atractylodis, 9.5 parts of cyrtomium fortunei, 13 parts of radix isatidis and 6 parts of radix sileris.

(6) Control group 5 (blank control group without any drug).

(3) Purchasing and processing of test drugs: all tested medicines are selected by a professional teacher from the market of traditional Chinese medicines in the Bozhou of Anhui, and are processed according to requirements. According to the test requirement, the medicines used in each group are respectively weighed, mixed and crushed to prepare powder, the powder is strictly numbered according to the group number, and the powder is packaged for standby.

(4) The test period is 60 days (two months)

(5) Dosage of the medicine used is as follows:

(1) the usage method is as follows: mix materials and feed. The first feeding is carried out for 15 days, the feeding is stopped for 10 days, the later feeding is carried out for 10 days, and the feeding is stopped for 10 days, and the use is repeated until the test is expired.

(2) The dosage is as follows: the experimental group is added according to the total formula dosage of 1.5% for the first time and 1% for the later time; control group 5, without any addition of ingredients; the other groups (control 1, 2, 3 and 4 groups) are added with mixed materials according to the dosage and usage after removing monarch, minister, adjuvant and conductant drugs.

(6) During the test, feed, drinking water, management, "tooth extraction" technique, disinfection and the like are normally performed.

(7) Observation records are made daily during the test period, problems occur, and a teacher decides to process comments in time.

2. And (3) result judgment:

determination criteria: the pigs are judged to be effective in prevention when no non-pestilence occurs during the test period; the onset of disease (positive by PCR) was judged as: preventing the failure.

3. The statistics of the test results are shown in Table 1:

table 1 results of the different formulations

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From the table above, it can be seen that: the experimental group uses the whole formula to achieve 95% of the effective rate of preventing the non-plague, and the effect is satisfactory; the effective rate of the contrast 1 group is 70%, which indicates that the effect of the monarch drug in the whole formula is very important; the effective rate of the contrast group 2 is 80%, which is important for demonstrating ministerial drugs and also demonstrating that the monarch and ministerial compatibility can improve the prevention effect; the effective rate of the contrast 3 group is 70%, which shows that the adjuvant materials of cyrtomium fortunei, radix isatidis, rhizoma atractylodis and radix sileris have certain importance in the formula, and cannot be seen little for inhibiting viruses, dispelling plague, dispelling dampness, dispelling wind and eliminating pathogenic factors, and are also indispensable for preventing non-plague components; the comparison group 4 uses the formula of the invention, which removes two guiding drugs of dried orange peel and liquorice, and has main drugs of monarch drug, minister drug and adjuvant drug, the effect of preventing non-pestilence is less affected, the effective rate reaches 80%, and the guiding drugs cannot be absent; control group 5, without any drug, was a blank. The results show that the effective rate of 4 cases and 36 cases are invalid, and the effective rate is only 10%, so that the composition has remarkable effects of improving the healthy qi and disease resistance immunity of pigs, can remarkably improve the threshold (threshold) of the pigs on infection of non-pestilence, has remarkable effect of preventing the non-pestilence, and is worthy of popularization.

Efficacy test (II)

The composition of the invention is prepared into granules:

The preparation method comprises the following steps: the traditional Chinese medicine composition is prepared by soaking and decocting raw material decoction pieces for three times, filtering, combining filtrate, concentrating into clear paste, and preparing granules (containing auxiliary materials) according to the mass ratio of the total weight of the composition to the prepared granules of 10:2.

Efficacy experimental case:

two adjacent pig farms (field A, field B, field 500) are located in suburban areas of Tang mountain, hebei.

The two pig farms in 2020 and 9 months are both in epidemic situation, clinical symptoms, pathological section detection changes and the like of the pig farms are suspected to be African swine fever, and then the pig farms are detected by a PCR laboratory, so that the pig farms are diagnosed as non-pestilence positive, and then the pig farms A are set as an experiment group, and the pig farms B are set as a control group and are treated by adopting different methods respectively:

a field (experimental group): there were 5 pigs in succession. Immediately adopting prevention and control measures to remove sick pigs and pigs with the same ring. After the pig house is cleaned, the pig house is sterilized at high temperature by a flame spray gun, other pig groups in the pig house are fed with the granules of the embodiment 2 of the invention, 4 kg of the granules are added into each ton of feed, and the continuous period is 15 days; after that, 3 kg of feed is added per ton of feed, and after all the feed is stabilized, the feed is reduced to 2 kg of feed per ton, so that the pig farm is stabilized, and no new non-pestilence cases occur.

B field (control): 3 pigs first developed, and started to show symptoms such as fever, no eating, redness of skin and the like. Local veterinarians can take a diagnosis and treatment at home, continuously take injections for the sick pigs twice a day, and the drugs mainly comprise: cephalosporins, sulfonamides, antipyretics, dexamethasone, vitamin c and the like, but more and more sick pigs die, more than 30 pigs die within 10 days, and healthy pigs are infected successively before. And then, under the guidance of a teacher in the hospital, uniformly performing innocent treatment on pigs dying of illness, strictly isolating the pigs from illness, controlling people flow, traffic flow and logistics, thoroughly and largely sterilizing the whole field, and starting feeding the granules of the composition of the embodiment 1 of the invention to the surviving pigs. The pig farm is stable gradually, and most pigs are kept without morbidity and mortality although few sporadic cases exist.

Efficacy test (three):

1. the experimental method comprises the following steps:

(1) The method comprises the steps of selecting 90 pigs of a non-pestilence positive (with toxicity) pig farm in Henan province, randomly dividing the pigs into three groups of 30 pigs, and respectively isolating and feeding, drinking water, managing, sterilizing and tooth extraction, wherein the application of the technology and the non-pestilence detection method are the same.

(2) Grouping and formula:

(1) experiment group (using a non-pestilence-preventing formulation provided by a veterinarian in the middle aged of one county, henan province Xu Changshi:

21 parts of codonopsis pilosula, 38 parts of astragalus, 19 parts of poria cocos, 21 parts of bighead atractylodes rhizome, 18 parts of Chinese yam, 16 parts of magnolia officinalis, 9 parts of champagne and 8 parts of elecampane

(2) Experiment two groups (using a middle veterinarian technical teacher in the city of the weekly port of Henan province to provide a non-pestilence resistant traditional Chinese medicine formulation:

35 parts of gypsum, 24 parts of honeysuckle, 18 parts of coptis chinensis, 18 parts of radix scutellariae, 16 parts of gardenia, 16 parts of radix rehmanniae, 15 parts of buffalo horn and 8 parts of liquorice

(3) Experiment three groups (using national invention patent application publication/publication number CN111514269a, african swine fever prevention traditional Chinese medicine composition):

25 parts of radix pseudostellariae, 35 parts of astragalus membranaceus, 50 parts of plaster of paris, 30 parts of rheum officinale, 20 parts of radix curcumae, 50 parts of cornu bubali and 35 parts of fructus forsythiae.

(3) Purchasing and processing of experimental medicines:

all the traditional Chinese medicine decoction pieces are selected by a professional teacher from the wholesale market of the traditional Chinese medicine in the Bozhou of Anhui. Respectively weighing, mixing, pulverizing, making into powder, and packaging according to number.

(4) Dosage of experimental medicine:

(1) the usage method is as follows: mix materials and feed.

(2) The dosage is as follows: 1. the two groups are used according to the dosage of the threatening pig group, each ton of feed is 18kg, 15 days are firstly used, and then 15kg are added to each ton of feed until the experiment is expired.

Third group: the feed additive was used according to the instruction, with a 5% amount per ton of feed, and was used continuously until the experiment was completed.

(5) Observation records are made daily during the experiment, problems occur, and the technical teacher decides to treat the comments in time.

2. And (3) result judgment:

3. The statistics of the experimental results are shown in Table 2:

table 2: experimental results statistics table for different formulas

As can be seen from table 2: the traditional Chinese medicine is effective in preventing African swine fever, but different compositions show different effects. The effective rate of the first formula reaches 77%; the effective rate of the second formula reaches 63%; the third formula has the effective rate reaching 80 percent, and has a larger gap compared with the effective rate of 95 percent.

Description of the preferred embodiments

Example 1:

the traditional Chinese medicine preparation for preventing African swine fever of the embodiment comprises the following raw materials in parts by weight:

The preparation method comprises the following steps: mixing the above materials, pulverizing, sieving, and mixing to obtain powder preparation.

Case: about 1000 or more pigs are in stock in Henan Hechang pig farm, wherein more than 40 pigs can be bred, 500 pigs can be bred, and 400 pigs can be fattened. About 12 days after 10 months in 2019, the fattening house sells pigs, 3 pigs are found in two circles of the fattening house, the body temperature is increased by about 41 ℃, the feeding is avoided, the spirit is poor, the lying place is motionless, the buttock skin is reddish, the skin has a plurality of blackened and necrotic parts, and one of the pigs is bloody. Immediately cleaning out the diseased pigs and the pigs with the same circles, thoroughly sterilizing the pigsty, immediately taking comprehensive prevention and control measures, prohibiting foreigners and vehicles from entering the field, performing primary sterilization in the field environment, and performing primary sterilization every day afterwards. And (3) killing three sick pigs, and performing innocent treatment after anatomical lesions completely conform to non-pestilence pathological changes. The powder of the product of the invention is added to pigs of other sizes in a pig farm, 15-20kg of the powder is added per ton of the powder, the feeding is continued for 15 days, 1% of the powder is added later, and the powder is continuously added for one month. Later the whole herd is stable and no new cases have occurred to date.

Such examples are still numerous locally.

Example 2:

according to parts by weight, the pure traditional Chinese medicine preparation for preventing African swine fever of the embodiment is powder prepared from the following traditional Chinese medicine raw materials.

The usage amount is as follows:

the usage method is as follows: mix materials and feed. The pig group is firstly used for 15 days, then is stopped for 10 days and used for 10 days, and the cycle is performed; the threatened swinery can be continuously used until the threat is relieved, and the swinery is stable; the pig group is reduced after being stabilized and can be added all the year round.

The dosage is as follows: the general pig group is added with 10-15kg of powder per ton of feed; the threatening pig group is added with 15-20kg of powder per ton of feed; after the pigs are stable, 5kg of feed is added every ton of feed every year.

Case: the method is characterized in that in the xxx pig farm in Guangdong MaoMing, epidemic situation occurs in more than 1300 pigs and 11 months in 2020 in each stage of the stock, the epidemic situation gradually spreads from the conservation house to the fattening house, and 20 times of diseases and symptoms occur in sequence: the body temperature is 40-41.6 ℃, the spirit is inappetence, the skin of the ears and buttocks is cyanoted, the respiration is quickened, the individual pig skin has different sizes of focal necrosis, and the feces or nostril bleeding is caused; the prominent lesions seen by the dissection were: general bleeding and swelling of the lymph nodes are in a purple grape shape, and bleeding spots with different sizes are formed on the liver, the kidney, the lung, the epicardium and the gastrointestinal serosa layer, and the swelling and bleeding of the spleen are obvious; PCR self-test, non-pestilence positive.

Under the guidance of a teacher in the hospital, the non-plague positive pigs and the same pigs are all removed immediately, and the pig house is thoroughly cleaned and disinfected; the leakage and the deficiency are comprehensively detected, measures are taken to block the loopholes, thoroughly disinfect the environment inside and outside the field, and the work is maintained for a long time. Meanwhile, other non-diseased pigs in the field are fed with the powder of the invention, 18kg of the powder is added into each ton of feed, and the feed is continuously fed for 15 days, thus basically controlling the occurrence of non-plague. After the feed is added with 10 kg per ton of feed and continuously fed for 10 days, the whole field is stable without new diseases, and after the feed is added with 5kg per ton of feed, the feed is used for a long time. To date, pig farms are stable and have no new non-blast cases.

Similar to the pig farm, tens of the traditional Chinese medicine preparation of the embodiment is used for preventing and comprehensively preventing and controlling teeth extraction, isolation, disinfection, biological safety and the like, so that the lives of more than hundred thousand pigs are guaranteed, contribution is made to increasing income of farmers, and the traditional Chinese medicine preparation is well-liked by farmers.

Example 3:

the traditional Chinese medicine preparation for preventing African swine fever of the embodiment comprises the following traditional Chinese medicine raw materials in parts by weight:

The preparation method comprises the following steps: mixing, crushing, sieving and remixing the traditional Chinese medicine raw materials to obtain the traditional Chinese medicine preparation with the formula.

The usage amount is as follows:

The dosage is as follows: the general pig group is added with 10-15kg of powder per ton of feed; the threatening pig group is added with 15-20kg of powder per ton of feed; after the pigs are stable, 5kg of feed is added every ton.

Case: the Zhan Jiang city xxx sow farm in Guangdong province stores more than 400 sows. Beginning in the next 3 th 2019, epidemic situation occurs in the surrounding pig farms, most of the pig farms are african swine fever, and some pig farms are blue-ear diseases, so that more than 80% of pig farms are non-pestilence and the pig farms are closed down, and the pig farms are strictly disinfected, people, vehicles, object approach and other prevention and control measures are strictly forbidden, and the emphasis is placed on protecting susceptible pig groups, the traditional Chinese medicine preparation is added into feed, and the traditional Chinese medicine preparation is added according to the amount of 8kg per ton of feed for long-term use. So far, the pig farm is stable and epidemic, and has no outbreak of non-plague and no blue ear disease.

Example 4:

according to parts by weight, the traditional Chinese medicine granule is prepared from the following traditional Chinese medicine raw materials by using the formula of the invention for preventing African swine fever.

The usage amount is as follows: mix materials and feed. According to the situation, 1-4 kg of the feed is added into each ton of the feed.

Case: a pig farm in Zhangzhou city of Fujian province stores 800 pigs. Epidemic situation happens in 5 months in 2019, and 11 patients die successively. The African swine fever is confirmed by local veterinary clinical diagnosis, in particular by PCR detection. Under the guidance of a teacher in our hospital, the pigs with diseases and the same circles are immediately and fixed-point removed, other pigs without diseases are timely fed with the traditional Chinese medicine preparation in the embodiment, 4 kg of the traditional Chinese medicine preparation is added into each ton of feed, the feeding is continuously carried out for 15 days, and biological safety measures such as environmental sanitation, disinfection, isolation, prevention of alien vehicles and the like are enhanced, so that the pig flock is stable after one week. Gradually reducing the weight to 2 kg of feed per ton, and feeding for a long time. So far, the pig farm is stable and epidemic-free.

After more than 2 years, the traditional Chinese medicine preparation is promoted and applied in places in the provinces of Henan, hebei, shanxi, shandong, guangdong, fujian and the like, fully proves that the traditional Chinese medicine preparation has good effect in preventing African swine fever, is welcomed and favored by pig farms (households), and contributes to guaranteeing the healthy development of pig industry.

The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims

1. A Chinese medicinal composition for preventing African swine fever is characterized by being prepared from astragalus mongholicus, ginseng, earth-fried bighead atractylodes rhizome, rhizoma atractylodis, dried orange peel, cyrtomium fortunei, radix isatidis, radix sileris and liquorice;

the weight portions of the raw materials are as follows:

26-35 parts of astragalus membranaceus, 5-12 parts of ginseng, 5-15 parts of ground-fried bighead atractylodes rhizome, 4-12 parts of rhizoma atractylodis, 5-10 parts of dried orange peel, 5-14.5 parts of cyrtomium fortunei, 8-18 parts of radix isatidis, 4-12 parts of radix sileris and 5-10 parts of liquorice;

the raw materials are Chinese medicinal decoction pieces.

2. The traditional Chinese medicine composition according to claim 1, wherein the weight parts of the raw materials are as follows:

28-33 parts of astragalus membranaceus, 7-10 parts of ginseng, 7-13 parts of ground-fried bighead atractylodes rhizome, 6-10 parts of rhizoma atractylodis, 6-10 parts of dried orange peel, 7-12.5 parts of cyrtomium fortunei, 11-15 parts of radix isatidis, 5-7.5 parts of radix sileris and 6-9 parts of liquorice.

3. The traditional Chinese medicine composition according to claim 1, wherein the weight parts of the raw materials are as follows:

4. The use of the traditional Chinese medicine composition according to any one of claims 1-3 in preparing a medicine for preventing african swine fever.

5. A medicament for preventing african swine fever, which is characterized by comprising the traditional Chinese medicine composition of any one of claims 1-3 and pharmaceutically acceptable auxiliary materials.

6. The medicament according to claim 5, wherein the dosage form is powder or granule.

7. The medicine according to claim 5, wherein the dosage form is a granule, and the pharmaceutically acceptable auxiliary materials in the granule comprise at least one of glucose, starch, flavoring agent and the like.

8. The medicament according to claim 7, wherein the preparation method of the granule comprises:

soaking the Chinese medicinal decoction pieces, decocting, filtering, mixing filtrates, concentrating into fluid extract, adding anhydrous glucose, starch, and essence, granulating, and oven drying;