CN114052019A

CN114052019A - Attractant for monitoring oriental fruit moth and application thereof

Info

Publication number: CN114052019A
Application number: CN202111203461.5A
Authority: CN
Inventors: 孔维娜; 王怡; 郭永福; 柴晓晗; 胡学丰; 李捷; 马瑞燕
Original assignee: Shanxi Agricultural University
Current assignee: Shanxi Agricultural University
Priority date: 2021-10-15
Filing date: 2021-10-15
Publication date: 2022-02-18

Abstract

The invention relates to the technical field of pest attraction, in particular to an attractant for monitoring oriental fruit moth and application thereof. The attractant comprises: sugar, glacial acetic acid and absolute ethyl alcohol; the mass ratio of the sugar to the glacial acetic acid to the absolute ethyl alcohol is 1: 3.15: 0.79 or 1: 1.05: 2.37. according to the invention, sugar, glacial acetic acid and absolute ethyl alcohol in a specific proportion are compounded, so that not only can a strong antennal potential reaction of the oriental fruit moth be caused under a meta-acid proportion, but also a strong directional flight reaction of the oriental fruit moth can be caused under a meta-alcohol proportion, and the monitoring potential of the oriental fruit moth is verified in the field. The embodiment shows that the attractant provided by the invention has the best effect on the antennal potential reaction and the aerodynamic behavior reaction of the unmated female moths, the unmated male moths and the white moth of the oriental fruit moths, and has obvious attraction effect.

Description

Attractant for monitoring oriental fruit moth and application thereof

Technical Field

The invention relates to the technical field of pest attraction, in particular to an attractant for monitoring oriental fruit moth and application thereof.

Background

The oriental fruit moth is a main economic pest, and mainly takes larvae boring into the young tips and fruits of the fruit trees of the Rosaceae family. Feces accumulated in the moth holes gradually turn black and rot to form black scars, which are commonly called as black plaster; when reaching the core, the fruit is eaten around the core and is convenient to eat, commonly called as the bean paste filling, so that the fruit falls off, is rotten and is not storage-resistant. When the damage is serious, the insect fruit rate can reach about 70 percent.

The traditional chemical pesticide for preventing and treating the oriental fruit moth has the problems of multiple times of application and improper time, has very poor prevention effect, and can cause a series of problems of pesticide residue, food safety, natural enemy killing and the like because the field hazard rate after prevention and treatment can still reach more than 50%. The biological attractant is used for monitoring or preventing the oriental fruit moth, and the effect of accurately applying chemical pesticides (including insecticides or ovicides) in a decrement way is obvious according to the occurrence rule of imagoes. However, the sex pheromone only aims at male moths at present, and the sex pheromone is lack of effective monitoring on female moths, particularly female moths with the pregnant eggs and the egg laying by diffusion.

Disclosure of Invention

In order to solve the problems, the invention provides an attractant for monitoring oriental fruit moth and application thereof. The attractant provided by the invention has good attraction effect on the male and female Grapholitha molesta Busck, plays a role in monitoring the male and female moths, and also plays a guiding role in other control means.

In order to achieve the above purpose, the invention provides the following technical scheme:

the invention provides an attractant for monitoring oriental fruit moth, which comprises the following components: sugar, glacial acetic acid and absolute ethyl alcohol; the mass ratio of the sugar to the glacial acetic acid to the absolute ethyl alcohol is 1: 3.15: 0.79 or 1: 1.05: 2.37.

preferably, the sugar comprises glucose.

Preferably, the attractant further comprises water; the mass ratio of sugar to water in the attractant is 1: 80.

the invention also provides the application of the attractant in the means for preventing and controlling the oriental fruit moth; the means for guiding the medicine control of the oriental fruit moth comprises the quantitative reduction application of the medicine.

Preferably, the oriental fruit moth drug comprises an oriental fruit moth insecticide or an oriental fruit moth ovicide.

The invention also provides application of the attractant in preventing and/or monitoring the oriental fruit moth.

The invention also provides a method for trapping oriental fruit moth, which comprises the following steps: putting the attractant into a carrier;

and (3) putting the carrier containing the attractant into a trapping device to trap the oriental fruit moth.

Preferably, the trap device comprises a basin trap.

Preferably, the carrier comprises a centrifuge tube.

Preferably, the spacing of the traps is > 30 m.

Has the advantages that:

the invention provides an attractant for attracting oriental fruit moth, which comprises the following components: sugar, glacial acetic acid and absolute ethyl alcohol; the mass ratio of the sugar to the glacial acetic acid to the absolute ethyl alcohol is 1: 3.15: 0.79 or 1: 1.05: 2.37. according to the invention, sugar, glacial acetic acid and absolute ethyl alcohol in a specific proportion are compounded, so that strong antennal potential reaction of the oriental fruit moth can be caused under the condition of a partial acid proportion, strong directional flight reaction of the oriental fruit moth can be caused under the condition of a partial alcohol proportion, and the compound pesticide has a good monitoring effect on the oriental fruit moth in the field. The embodiment shows that the attractant provided by the invention has the best effect on the antennal potential reaction and the aerodynamic behavior reaction of the unmated female moths, the unmated male moths and the white moth of the oriental fruit moths, and has obvious attraction effect.

Drawings

FIG. 1 shows the response values of the antennal potential (EAG) of the unmated female moth, the unmated male moth and the female moth of the pregnant egg to different sugar-vinegar wine formulas;

FIG. 2 shows the behavioral response rates of the unmated female moths, the unmated male moths and the female moths with eggs of the oriental fruit moths to the directional flight of different sugar-vinegar wine formulas;

FIG. 3 shows the behavioral response rates of the unmatched female moths, the unmatched male moths and the female moths with eggs for the surrounding luring flying of different sugar and vinegar wine formulas;

FIG. 4 shows the field trapping situation of Grapholitha molesta female moth and male moth by different sweet and sour liquor formulas; different lower case letters above the bar graph indicate significance of difference within different genders; "+" indicates that the trapping amount of male moth and female moth is different;

FIG. 5 shows the response values of the antennal potential (EAG) of the positive polar moth, the positive polar moth and the female egg moth of the oriental fruit moth to different attractants;

FIG. 6 shows the behavior response rates of the unmated female moths, the unmated male moths and the female moths with eggs for the surrounding attraction flying of different attractants of the oriental fruit moth;

FIG. 7 shows the field trapping situation of Grapholitha molesta female moths and male moths by different attractants.

Detailed Description

The invention provides an attractant for attracting oriental fruit moth, which comprises the following components: sugar, glacial acetic acid and absolute ethyl alcohol; the mass ratio of the sugar to the glacial acetic acid to the absolute ethyl alcohol is 1: 3.15: 0.79 or 1: 1.05: 2.37.

unless otherwise specified, the present invention does not require any particular source for each component of the attractant, and any commercially available product known to those skilled in the art may be used.

The attractant comprises 1 part by mass of sugar. In the present invention, the sugar preferably includes glucose. The sugar provided by the invention has the effect of attracting Grapholitha molesta Busck as sweet bait, and particularly has a good attraction effect on both female moths and male moths of Grapholitha molesta Busck.

The attractant comprises 1.05 parts by mass or 3.15 parts by mass of glacial acetic acid based on the parts by mass of the sugar, and more preferably 1.05 parts by mass. In the present invention, the density of glacial acetic acid is preferably 1.05g/cm³. The glacial acetic acid can be used as food or oviposition olfactory clues of the oriental fruit moth and has the function of attracting the oriental fruit moth.

The attractant comprises 0.79 part by mass or 2.37 parts by mass of absolute ethyl alcohol based on the mass part of the sugar, and more preferably 2.37 parts by mass. In the present invention, the density of the absolute ethanol is preferably 0.79g/cm³. The glacial acetic acid has the function of remote signals, and can help release the smell of sugar and glacial acetic acid, so that the olfactory reaction of the oriental fruit moth is further promoted.

According to the invention, the sugar, the glacial acetic acid and the absolute ethyl alcohol in a specific ratio are compounded to cause strong antennal potential and directional flight reaction of the oriental fruit moth female and the male moth, so that the oriental fruit moth female and the male moth can be effectively monitored in the field.

The attractant of the present invention preferably further comprises 80 parts by mass of water, based on the parts by mass of the sugar. In the present invention, the density of the water is preferably 1g/cm³. According to the invention, through compounding the unmated female moths, the unmated male moths and the pregnant female moths of the oriental fruit moths with water in a specific ratio, the obtained attractant has the best effects on the antennal potential reaction and the aerodynamic behavior reaction of the unmated female moths, the unmated male moths and the pregnant female moths of the oriental fruit moths; in addition, the tropism of the female moth to the attractant is obviously higher than that of the male moth, the female moth is firstly attracted to the vicinity by the attractant, and the male moth can also be attracted by the female moth in the vicinity of the attractant, so that the attraction effect of the male moth is enhanced.

The invention also provides application of the attractant in guiding the means for preventing and controlling the oriental fruit moth. In the present invention, the means for directing the medicinal control of oriental fruit moth preferably comprises the application of a drug in a quantitative reduction manner; the oriental fruit moth medicament preferably comprises an oriental fruit moth insecticide or an oriental fruit moth ovicide. The attractant provided by the invention has the best effect on the antenna potential reaction and the aerodynamic behavior reaction of the grapholitha molesta, and the grapholitha molesta attractant prepared by utilizing the characteristic provides data support for other prevention and control means such as medicament reduction application and the like.

The invention also provides application of the attractant in preventing and/or monitoring the oriental fruit moth. In the invention, the oriental fruit moth preferably comprises oriental fruit moth female moth and/or oriental fruit moth. The attractant provided by the invention has good attraction effect on the oriental fruit moth and the male moth, can be used for preventing and/or monitoring oriental fruit moth, and has the effects of reducing oriental fruit moth population and remarkably monitoring; in addition, by utilizing the green prevention and control technology represented by the sweet and sour wine liquid, the pesticide reduction can be achieved, the method has important significance for improving the ecological environment safety, and the sustainable development of agriculture is facilitated.

The invention also provides a method for trapping oriental fruit moth, which comprises the following steps: putting the oriental fruit moth attractant into a carrier;

and putting the carrier containing the oriental fruit moth attractant into a trapping device to trap female moths and/or male moths of the oriental fruit moth.

In the present invention, the carrier preferably comprises a centrifuge tube; the centrifugal tube preferably has an aperture structure, and more preferably, the tube cover of the centrifugal tube is provided with the aperture structure; the volume of the centrifugal tube is preferably 1.5 mL; the liquid loading amount of the attractant is preferably 1 mL. According to the invention, the attractant is loaded into the carrier, so that the attractant can be prevented from directly contacting with the trapping device, and the trapping effect is influenced; in addition, the attractant is filled into the carrier, so that the attracting cost can be reduced, and the trapping of the natural enemies of the oriental fruit moth can be avoided; moreover, the aperture structure is arranged in the centrifugal tube, so that effective components in the attractant can be volatilized, and the attraction effect is achieved.

In the present invention, the trap preferably comprises a basin trap; the diameter of the basin trap is preferably 20cm, and the height of the basin trap is preferably 10 cm; the height of water filled in the basin trap is preferably 8-9 cm; the basin trap preferably further comprises washing powder; the mass ratio of the washing powder to the water contained in the basin trap is preferably 1: 50; the invention can reduce the surface tension of water by mixing the washing powder with the water, thereby preventing the trapped moths from escaping.

In the present invention, the distance of the carrier from the water surface in the basin trap is preferably 1 cm; the trap is preferably 1.5m from the ground; the spacing of the traps is > 30 m. The invention can reduce the interference among the traps by setting the proper interval of the traps, thereby improving the trapping effect.

To further illustrate the present invention, an attractant for monitoring grapholitha molesta and its application provided by the present invention will be described in detail with reference to the following examples, which should not be construed as limiting the scope of the present invention.

Example 1

A grapholitha molesta attractant, the attractant being: 1g of glucose and 3.15g of glacial acetic acid (density 1.05 g/cm)³) And 0.79g of absolute ethyl alcohol (density of 0.79 g/cm)³)。

Example 2

A grapholitha molesta attractant, the attractant being: 1g of glucose and 1.05g of glacial acetic acid (density 1.05 g/cm)³) And 2.37g of absolute ethyl alcohol (density 0.79 g/cm)³)。

Example 3

A grapholitha molesta attractant, the attractant being: 1g of glucose and 3.15g of glacial acetic acid (density 1.05 g/cm)³) 0.79g of absolute ethyl alcohol (density of 0.79 g/cm)³) And 80g of water (density 1 g/cm)³)。

Example 4

A grapholitha molesta attractant, the attractant being: 1g of glucose and 1.05g of glacial acetic acid (density 1.05 g/cm)³) 2.37g of absolute ethyl alcohol (density 0.79 g/cm)³) And 80g of water (density 1 g/cm)³)。

Application example 1

The grapholitha molesta does not mate female moths, male moths and female moths from pregnancy to the EAG of different sugar-vinegar wine formulas.

And collecting the feelers at the head of the moth by using a micro-scissors, cutting off the tail end of the feeler, saving the feeler by 0.1mm, and connecting the feelers and the tail end of the feeler between fork-shaped metal electrodes of the EAG probe PRG-3 by using conductive adhesive. The electrodes at the antenna ends are connected to a signal acquisition controller SYNTECHIDAC-2, which is connected to the EAGv2.6 software. Stimulation was started after baseline plateaus. The continuous gas flow is 200mL/min, the pulse stimulation gas flow is 20mL/min, the pulse interval-stimulation duration is 0.3s, and the stimulation interval is 1 min. Dripping 10 μ l of sugar and vinegar liquor to be measured on the strip-shaped filter paper, completely pushing into 1000 μ l of pipette tip, connecting to the stimulus source conduit, and introducing the tip of pipette tip into the main airflow tube. One antenna was used per female, the same antenna was stimulated only once, and each treatment was repeated 6 times. The test is carried out from 6 months to 8 months in 2017 and from 6 months to 8 months in 2018, the average value of the two tests is taken, and the specific test result is shown in table 2 and figure 1.

TABLE 1 sweet and sour wine for luring oriental fruit moth and 9 kinds of formula

TABLE 2 response values of the antennal potential (EAG) of the unmatched female moth, the unmatched male moth and the female moth of the Huai ovum to different sugar-vinegar wine formulas

Formulation of	Unmated male	Unmated female	Pregnant female
				#1	1.95±0.07b	1.24±0.04d'	1.51±0.34a”
#2	4.86±0.22d	5.32±0.51d'e'	4.87±1.04b”c”
				#3	6.91±0.25e	6.50±0.13e'	5.64±0.52c”
#4	2.55±0.12bc	2.69±0.13b'c'	2.46±0.17a”b”
				#5	4.39±0.26d	3.66±0.52b'c'd'	5.37±1.02b”c”
#6	7.37±0.25e	5.61±0.28d'e'	6.68±1.29c”
				#7	1.38±0.14a	2.16±0.24a'b'	3.69±0.28b”c”
#8	2.19±0.26b	3.78±0.53b'c'd'	5.14±0.66b”c”
				#9	3.28±0.32c	4.53±1.16c'd'e'	4.21±0.74b”c”

As can be seen from table 2 and fig. 1, there was a significant interaction between different kava formulations and different moth-forming types in the moth-forming EAG reaction (F: 2.868, df: 16, P < 0.05). The differences of the response of the 9 formulas to the EAG were not significant (F ═ 2.522, df ═ 2, and P ═ 0.084). However, the three moth-forming types showed significant differences in EAG responses to 9 cider formulations (F35.57, df 8, P < 0.05). The EAG response of adult moths to formulations #1 and #7 was significantly lower than for formulations #3 and # 6. The virgin male moths had a higher EAG response to formulas #3 and #6 than formula #7 (F-81.168, df-8, 53, P < 0.05). The virgin female moth responded to EAG higher than formulation #1 (F-12.838, df-8, 53, P <0.05) to formulation # 3. Pregnant females had higher EAG response to formulations #3 and #6 than formulation #1(F ═ 5.9, df ═ 8,53, P < 0.05).

Application example 2

The grapholitha molesta is not copulated by female moths, male moths and female moths from pregnancy eggs to react with the pneumatic behaviors of different sugar-vinegar wine formulas.

The wind tunnel main body device is an organic silicon plastic cuboid with the thickness of 50cm multiplied by 150cm, filtered air is provided by a fan set to be 0.3cm/s through a pneumatic inlet, and air inside the wind tunnel is conveyed to the outside of a laboratory through a sealed pipeline. The wind-driven top irradiates 250Lx diffused red light, the room temperature is kept at 23 +/-2 ℃, and the relative humidity is kept at 50-70%. The adult moth is moved into a pneumatic chamber 2h before each test, and the test is started 2h before the natural dark period is started and lasts for 3 h. The two supports are placed 10cm away from the two ends of the wind tunnel and 30cm away from the bottom of the wind tunnel. The absorbent cotton ball with 10 μ L of sugar-vinegar liquor added is placed on the upper wind end support, and a release cage (diameter 7cm, height 9cm) containing 10 adult moths is placed on the lower wind end support, with the mouth of the release cage facing the wind-driven upwind end. One set of experiments was conducted for 20min, and two sets of experiments were ventilated at 20min intervals, and all adult moths were used once, and 6 sets were repeated. The typical behavior of adult moth individuals was determined: (1) after leaving the release cage, the aircraft was flown in a directed manner, and the results are shown in Table 3 and FIG. 2; (2) results of the circle lure flight measurements are shown in table 4 and fig. 3. The test is carried out from 6 months to 8 months in 2017 and from 6 months to 8 months in 2018, the average value of the two tests is taken, and the specific test result is shown in table 3, table 4, figure 2 and figure 3.

TABLE 3 behavioral response rates of the unpaired female moth, the unpaired male moth, and the pregnant female moth of Grapholitha molesta to the directional flight of different sugar-vinegar wine formulations

Formulation of	Unmated male	Unmated female	Pregnant female
				#1	1.67±1.67a₁	10.00±3.65b₁	15.00±2.24b₁
#2	0.00±0.00a₂	3.33±3.33a₂b₂	13.33±4.94b₂
				#3	0.00±0.00a₃	8.33±6.54a₃b₃	11.67±3.07b₃
#4	1.67±1.67a₄	5.00±3.42a₄	20.00±3.65b₄
				#5	1.67±1.67a₅	6.67±4.22a₅	13.33±4.94a₅
#6	1.67±1.67a₆	1.67±1.67a₆	5.00±2.24a₆
				#7	1.67±1.67a₇	5.00±3.42a₇	16.67±5.58b₇
#8	3.33±2.11a₈	11.67±4.77a₈	16.67±4.94a₈
				#9	1.67±1.67a₉	1.67±1.67a₉	11.67±6.54a₉

TABLE 4 behavioral response rates of the unpaired female moth, unmated male moth, and white egg female moth to the surrounding luring flight of different sugar and vinegar wine formulas

As can be seen from tables 3, 4, 2 and 3, there was no significant interaction between different formula of the sweet and sour liquors and different types of the moth-forming species in both the moth-forming directional flight and the surrounding lure flight reactions (directional flight: F0.542, df 16, P0.92; surrounding lure flight F1.088, df 16, P0.372). The behavioral response difference of the adult moths to the directional flight and the surrounding attraction flight of 9 sweet and sour liquor formulas is not obvious (the directional flight: F is 1.679, df is 8, and P is 0.109; the surrounding attraction flight: F is 0.929, df is 8, and P is 0.494). However, in each formula of the sweet and sour wine, the behavioral responses of 3 moth-forming type directional flights and surrounding lure flights were significantly different (directional flight: F: 31.845, df: 2, P < 0.05; surrounding lure flight: F12.473, df: 2, P < 0.05).

As can be seen from table 3 and fig. 2, the response rate for the unmated female moths was generally higher than that for the unmated male moths and lower than that for the pregnant female moths for the directional flights. For formulas #1 (F-7.729, df-2, 17, P <0.05), #2 (F-4.626, df-2, 17, P <0.05) and #3 (F-4.39, df-2, 17, P <0.05), female white moths responded more than the virgin males, but they all differed insignificantly from the virgin females. The female white-egg moth responded to formulations #4 (F-10.994, df-2, 17, P <0.05) and #7 (F-4.539, df-2, 17, P <0.05) more than the female and male virgin moths. However, there was no significant difference in the reaction rate between formulations #5(F ═ 2.075, df ═ 2,17, P ═ 0.16), #6(F ═ 1.053, df ═ 2,17, P ═ 0.373), #8(F ═ 2.534, df ═ 2,17, P ═ 0.113) and #9(F ═ 1.793, df ═ 2,17, P ═ 0.2) that differed in the moth type.

As can be seen from table 4 and fig. 3, the response rate of the unmated female moths was generally higher than that of the unmated male moths and the female moths with eggs for the round lure flights. Formulations #5 (F-10, df-2, 17, P <0.05) and #1 (F-5.509, df-2, 17, P <0.05) obeyed this rule. Different moth-forming types have no significant difference in the reaction rate of formulation #2(F is 0.5, df is 2,17, P is 0.616), #3(F is 1.304, df is 2,17, P is 0.3), #4(F is 2.17, df is 2,17, P is 0.149), #6(F is 2.394, df is 2,17, P is 0.149), #7(F is 1, df is 2,17, P is 0.125), #7(F is 1, df is 2,17, P is 0.391), #8(F is 1.644, df is 2,17, P is 0.226, #9(F is 0.238, df is 2,17, P is 0.791> 0.05).

Application example 3

The different sugar-vinegar wine liquid formulas can trap the oriental fruit moth and the male moth in the field.

The field test site is located in a peach orchard of 15 hectares in west Shanbei village in Taigu area of Shanxi province, jin Zhong City, and the test is carried out in two main peak periods of 6-8 months of adult moths in 2019. The peach tree is cultivated by conventional farming management, wherein the peach tree is a long-term guarantee (the height is 2.5m, the row spacing is 2.5m, and the row spacing is 5 m). A red basin trap (with the diameter of 20cm and the height of 10cm) is adopted, 8-9 cm of water is filled in a basin, and 2 wt% (mass percentage of the water in the basin) of washing powder is added into the water. The traps are suspended from the branches about 1.5m from the ground, with the traps spaced at least 30m apart to reduce interference between traps. All treatments and controls were repeated 4 times according to a completely random design. Counting the moth trapping amount at 07: 00-10: 00 every day, and simultaneously removing moth trapping and sundries and adding water. The lure was changed 7 days after the lure at each peak, and each peak test was carried out for 14 days, with the test results shown in table 5 and fig. 4.

TABLE 5 field trapping situation of Grapholitha molesta Busck female moth and male moth by different sweet and sour liquor formula

Formulation of	Female part	Male part
			#1	0.00±0.00a	9.00±3.94a'b'
#2	0.00±0.00a	3.50±1.19a'b'
			#3	1.00±0.71a	15.25±8.23a'b'
#4	0.00±0.00a	5.00±2.04a'b'
			#5	0.25±0.25a	3.75±1.38a'b'
#6	1.00±0.41a	4.75±1.80a'b'
			#7	1.55±0.39a	25.10±9.6b'
#8	0.50±0.29a	2.75±1.03a'b'
			#9	0.50±0.29a	9.50±4.09a'b'
No induced source illumination	0.25±0.25a	2.00±1.68a'

As can be seen from table 5 and fig. 4, the attracting effect of the 9 sweet and sour wine formulas on female moths was not significantly different from that of the control (F ═ 1.889, df ═ 9,39, P ═ 0.093> 0.05). However, formulation #7 had significantly higher trapping effect on male moths than the control (F ═ 2.467, df ═ 9,39, P < 0.05). Formulations #1 (t-3.663, df-3, P <0.05), #4 (t-5.064, df-3, P <0.05), #5 (t-3.489, df-6, P <0.05), #6 (t-2.521, df-6, P <0.05), #7 (t-3.546, df-6, P <0.05) were significantly higher than those of males, and formulations #2 (t-2.984, df-3, P-0.058), #3 (t-2.282, df-3.845, P-087), #8 (t-2.431, df-6, P-0519, P-9, P-982, t-3, P-982, P-3, P-982, P-3, P-988, P-3, P-982, P-3, P-988, P-3, P-982, P-3, P-9, and P-9, and P-9, and controls. The field trapping test further proves that the attractant prepared in the embodiment 3 and the embodiment 4 has the best effect on the antenna potential reaction and the aerodynamic behavior reaction of the oriental fruit moth, can be used for preventing and/or monitoring the oriental fruit moth, particularly has a remarkable effect on monitoring the oriental fruit moth, can achieve pesticide reduction through the utilization of a green prevention and control technology represented by sugar-vinegar wine liquid, has an important significance on improving the ecological environment safety, and is beneficial to the sustainable development of agriculture.

Comparative example 1

A grapholitha molesta attractant, the attractant being: 3g of soft sugar, 1mL of glacial acetic acid, 3mL of absolute ethyl alcohol and 80mL of water.

Comparative application example 1

An assay similar to that of application example 1 was conducted except that the attractants to be tested were the lure prepared in comparative example 1 and the lure prepared in example 3, respectively, and the results of the assay are shown in Table 6 and FIG. 5.

TABLE 6 response values of antennal potential (EAG) of the unpaired female moth, the unpaired male moth and the white moth of oriental fruit moth to different attractants

Formulation of	Unmated male	Unmated female	Pregnant female
				Comparative example 1	2.09±0.18a	6.91±0.32b	5.59±0.33b
Example 3	6.91±0.25b	6.50±0.13b	5.64±0.52b

As can be seen from table 6 and fig. 5, the moth-forming antenna potential responses of the attractants prepared in comparative example 1 and the attractants prepared in example 3 are significantly different (F: 58.381, df: 5,35, P < 0.05). The feeler potential reaction of the male moth caused by the attractant prepared in the embodiment 3 is obviously higher than that of the attractant prepared in the comparative example 1.

Comparative application example 2

An assay similar to that of application example 2 was conducted except that the attractants to be tested were the lure prepared in comparative example 1 and the lure prepared in example 3, respectively, and the results of the assay are shown in Table 7 and FIG. 6.

TABLE 7 behavioral response rates of the unpaired females, unmated males and females of the oriental fruit moth to the flying around lures of different attractants

Formulation of	Unmated male	Unmated female	Pregnant female
				Comparative example 1	5.00±3.42a	0.00±0.00a	0.00±0.00a
Example 3	1.67±1.67a	6.67±3.33a	1.67±1.67a

As can be seen from table 7 and fig. 6, the attractant prepared in comparative example 1 and the attractant prepared in example 3 caused no significant difference in moth-formation surrounding attraction (F ═ 1.612, df ═ 5,35, P ═ 0.187) reactions. The sweet and sour wine liquid formula can cause the behavior of the female moths flying around the luring source. Meanwhile, the sweet and sour wine liquid can cause the surrounding luring flying behavior of the unmated female moths and the pregnant female moths.

Comparative application example 3

An assay similar to that of application example 3 was conducted except that the attractants to be tested were the lure prepared in comparative example 1 and the lure prepared in example 3, respectively, and the results of the assay are shown in Table 8 and FIG. 7.

TABLE 8 field trapping situation of Grapholitha molesta female moth and male moth by different attractants

Formulation of	Male part	Female part
			Comparative example 1	5.00±2.27ab	1.25±0.48b
Example 3	15.25±8.23a	1.00±0.71b

As can be seen from table 8 and fig. 7, the attractant prepared in comparative example 1 and the attractant prepared in example 3 had a significant difference in attracting effect to adult moths in the field (F ═ 3.384, df ═ 3,15, and P ═ 0.05). The attraction effect of the sweet and sour wine liquid formula on male moths is higher than that of the comparative sweet and sour wine liquid formula.

In conclusion, the compound attractant has the best effect on the antennal potential reaction and the aerodynamic behavior reaction of the unmated female moths, the unmated male moths and the white eggs of the oriental fruit moths by compounding the sugar, the glacial acetic acid and the absolute ethyl alcohol in a specific ratio, and has a remarkable attracting effect.

Although the present invention has been described with reference to the preferred embodiments, it should be understood that various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims

1. An attractant for monitoring grapholitha molesta, said attractant comprising: sugar, glacial acetic acid and absolute ethyl alcohol; the mass ratio of the sugar to the glacial acetic acid to the absolute ethyl alcohol is 1: 3.15: 0.79 or 1: 1.05: 2.37.

2. a lure according to claim 1, wherein the sugar comprises glucose.

3. An attractant as claimed in claim 1 wherein said attractant further comprises water; the mass ratio of sugar to water in the attractant is 1: 80.

4. use of the attractant of any one of claims 1 to 3 for guiding a means of medicinal control of Grapholitha molesta Busck; the means for guiding the medicine control of the oriental fruit moth comprises the quantitative reduction application of the medicine.

5. The use of claim 4, wherein the Grapholitha molesta drug comprises a Grapholitha molesta insecticide or a Grapholitha molesta ovicide.

6. Use of the attractant of any one of claims 1 to 3 for controlling and/or monitoring grapholitha molesta.

7. A method for trapping Grapholitha molesta Busck, which is characterized by comprising the following steps: placing the attractant of any one of claims 1-3 into a carrier;

8. The method of claim 7, wherein the trap device comprises a basin trap.

9. The method of claim 8, wherein the carrier comprises a centrifuge tube.

10. Method according to claim 7 or 8, wherein the spacing of the traps is > 30 m.