CN114009615A - Disease-resistant sow feed and preparation method thereof - Google Patents

Disease-resistant sow feed and preparation method thereof Download PDF

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Publication number
CN114009615A
CN114009615A CN202111412811.9A CN202111412811A CN114009615A CN 114009615 A CN114009615 A CN 114009615A CN 202111412811 A CN202111412811 A CN 202111412811A CN 114009615 A CN114009615 A CN 114009615A
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parts
weight
powder
mixed
beet pulp
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郜丽欢
孙宇
刁小东
王雨涵
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Hefei Shengjia Biotechnology Co ltd
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Hefei Shengjia Biotechnology Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/20—Animal feeding-stuffs from material of animal origin
    • A23K10/22—Animal feeding-stuffs from material of animal origin from fish
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/142—Amino acids; Derivatives thereof
    • A23K20/147—Polymeric derivatives, e.g. peptides or proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/174—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/28—Silicates, e.g. perlites, zeolites or bentonites
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/20—Inorganic substances, e.g. oligoelements
    • A23K20/30—Oligoelements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses a disease-resistant sow feed and a preparation method thereof. The disease-resistant sow feed contains the raw materials of the mixed fermentation product, the sugar/beet pulp polypeptide, the mixed forage powder, the mixed protein powder and the compound vitamin which have synergistic effect, participates in the physiological activity and the metabolic regulation of organisms, can enhance the immunity of the organisms, improve the disease resistance rate and further promote the growth of pigs. The disease-resistant sow feed contains rich nutrient substances, can effectively improve the immunity, disease resistance rate and feed intake rate of sows, and has the advantages of wide raw material source, low price and no toxic or side effect. The sow feed prepared by the method has good palatability, provides balanced nutrition for pigs, and supplements microelements such as selenium and calcium for pigs.

Description

Disease-resistant sow feed and preparation method thereof
Technical Field
The invention belongs to the technical field of animal feed preparation, and particularly relates to a disease-resistant sow feed and a preparation method thereof.
Background
The breeding industry accounts for an important proportion in the national life, the feeding problem of sows is also important in live pig breeding, and the health and the growth of piglets are directly influenced by the growth quality of the sows, so that the economic benefit is further influenced. The sow is easy to be affected by factors such as environment, nutrients, management and the like in the feeding process, so that the sow has some common diseases. Therefore, scientific breeding is required in the breeding process.
The Chinese invention patent CN 104171387A discloses a disease prevention and treatment sow feed and a preparation method thereof, the disease prevention and treatment sow feed comprises the following components: comprises flour, bean curd residue, tea seed cake, corn flour, wheat bran, soybean stem and leaf, vinasse, sweet potato leaf, violet, broccoli, lactobacillus leaven, fish meal, cloth residue leaf, shrimp meat, 6-12 of mushroom, 25-30 of rice bran, 1-2 of radish seed, scutellaria baicalensis, radix stephaniae tetrandrae, pteris multifida, polygonum hydropiper, purslane, white sugar, fennel, rice husk powder, a phagostimulant and a proper amount of water. The feed disclosed by the invention is rich in nutrition and strong in disease resistance, and is an economic sow feed which is safe, reliable, economic, environment-friendly and harmless. However, the feed for preventing and treating diseases of the sows has single nutrient component, the absorption effect of the sows is not good, the nutrition is seriously lost in the sows, and meanwhile, the diseases such as milk fever and diarrhea are not treated.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a disease-resistant sow feed and a preparation method thereof.
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 40-60 parts of mixed fermentation product, 20-40 parts of mixed forage powder, 5-15 parts of mixed protein powder, 1-3 parts of composite vitamin and 15-25 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the grain size is 0.5-1.5 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 30-50 parts of corn flour, 30-50 parts of sorghum flour, 15-25 parts of wheat bran, 10-20 parts of bean curd residue and 30-50 parts of water in parts by weight to obtain a coarse mixture;
s2: uniformly mixing 8-20 parts by weight of modified zeolite powder, 0.4-0.6 part by weight of bacillus subtilis and 80-120 parts by weight of the crude mixture obtained in the step S1 to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 30-40 hours at 23-27 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 400-420 ℃ at the initial temperature of 20-30 ℃ at the heating rate of 4-6 ℃/min, keeping for 1-3 hours, then cooling to 70-90 ℃ at the cooling rate of 8-12 ℃/min, transferring to 4-6 ℃ and keeping for 20-40 minutes to obtain physically modified zeolite powder;
t2: adding 15-25 parts by weight of the physically modified zeolite powder obtained in the step T1 into 40-60 parts by weight of the modification solution, uniformly mixing, soaking at 50-70 ℃ for 2-4 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (4) transferring the mixture A obtained in the step T2 to an oven at 50-70 ℃ for drying for 8-12 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 4-6 parts by weight of silane coupling agent, 15-25 parts by weight of sulfur-containing amino acid and 5-7 parts by weight of functional yeast liquid into 40-60 parts by weight of water, and uniformly mixing to obtain the modified liquid.
The silane coupling agent is at least one of vinyltriethoxysilane, vinyltrimethoxysilane and vinyltris (beta-methoxyethoxy) silane. Preferably, the silane coupling agent is vinyltriethoxysilane.
The sulfur-containing amino acid is at least one of methionine, cystine and cystine. Preferably, the sulfur-containing amino acid is methionine.
The zeolite powder has a pore structure, has adsorption and ion exchange effects on toxins, but has insufficient specific surface area and limited adsorption capacity. The zeolite powder is thermally modified, the temperature is raised, the moisture and organic matters in the zeolite powder are removed, so that the pore passages of the zeolite powder are unobstructed, the specific surface area is increased, the adsorption effect and the ion exchange performance of the zeolite powder are fully exerted, and then the zeolite powder is subjected to quenching treatment to form thermal shock, so that impurities are easily separated, the stability of the pore structure is facilitated, and the collapse of pores is prevented; the zeolite powder modified by selenium-calcium-rich yeast and sulfur-containing amino acid is used, the zeolite powder contacts with amino group in the sulfur-containing amino acid, the sulfur-containing amino acid is fixed on the surface of the zeolite powder by a coupling agent, the selenium in the selenium-calcium-rich yeast replaces the sulfur in the sulfur-containing amino acid to form seleno-amino acid, the calcium-chelated amino acid in the selenium-calcium-rich yeast forms amino acid chelated calcium, and after the amino acid is fixed on the surface of the zeolite powder, selenium and calcium elements are fixed on the amino acid, so that the surface modification of the zeolite powder is realized, the silicon content on the surface of the zeolite powder can be reduced, the porosity is improved, the average crystal and particle size of the silicon-containing zeolite powder material are reduced, and the porosity and ion exchange performance of the zeolite powder are improved. Sodium sulfite is formed by sodium and oxygen left by substituted sulfur and sodium selenite raw materials, the sodium sulfite is incompletely ionized under an acidic condition, the ion exchange performance of the zeolite powder is modified, a certain promotion effect on an ionization reaction can be achieved, sodium bisulfite is generated, the sodium bisulfite can be used for chemical detoxification of mycotoxin in sow feed, harm of joint protection mycotoxin to pig intestines can be effectively relieved, healthy intestines are kept, a proper amount of selenium element and calcium element are added, the foraging and digestion capacity of sows is improved, the number of lactation and the lactation quality of sows are increased while the organism health of the sows is improved.
The modified zeolite powder has a honeycomb network crystal structure, enters a pig body through an oral cavity, is absorbed by intestinal mucosa and enters blood and lymph tissue, has higher specific surface area and adsorption capacity, can adsorb microorganisms such as low molecular compounds, toxic compounds, mycotoxin and the like in the body, dissolves some small molecular substances and discharges the small molecular substances out of the body; the modified zeolite powder can be used as an adsorbent of heavy metals to adsorb lead, chromium, copper and the like through cation exchange characteristics, and the antibacterial and anti-diarrhea functions of the sow feed added with the modified zeolite powder are obviously enhanced; after the sow parturites, the requirement of milk for calcium is suddenly increased, the secretion of parathyroid hormone and calcitonin cannot adapt to the sudden change, postpartum paralysis is caused when calcium is lacked, namely, the symptoms of milk fever are caused, the sow feed added with the modified zeolite powder improves the resistance of the sow and can also participate in adjusting the calcium stabilizing matrix of the postpartum sow, the ash content, the calcium content and the phosphorus content and the strength of the cavity bone are increased, and the calcium content in serum is adjusted, so that the occurrence of milk fever is avoided, the effective nipple number is increased, and the milk secretion quality is improved.
The preparation method of the functionalized yeast liquid comprises the following steps: inoculating 1-2 parts of yeast to 90-110 parts of liquid culture medium by weight, and culturing at 28-30 ℃ for 1-2 days to obtain a functionalized yeast liquid;
the liquid culture medium comprises, by weight, 10-15 parts of honey, 10 parts of a functional agent, 0.5-1 part of glucose, 0.3-0.5 part of peptone and 80-100 parts of water.
The functional agent is FeSO4、CuSO4、ZnSO4、MnSO4、MgSO4、NaSeO3KI and CaCl2At least one of (1). Preferably, the functional agent is NaSeO3And CaCl2The mixture is composed according to the mass ratio of 1 (1.5-2.0).
Selenium and calcium elements are added into the selenium-calcium-enriched yeast in the process of culturing the yeast, and the selenium and calcium are absorbed and utilized during the growth of the yeast, so that the selenium and calcium are organically combined with protein and polysaccharide in the yeast body and converted into biological selenium and biological calcium, thereby eliminating chemical selenium and chemical calcium, and avoiding toxic and side effects on pig organisms and gastrointestinal stimulation. The yeast is rich in protein, sugar and 18 vitamins, and can be used as a selenium source and a calcium source and also provide other beneficial nutrients.
The preparation method of the mixed forage powder comprises the following steps: according to the weight portion, 10-30 portions of pasture, 3-7 portions of caulis spatholobi, 3-7 portions of acanthopanax sessiliflorus, 3-7 portions of cowherb seed, 8-12 portions of dandelion and 3-7 portions of rehmannia are crushed by a beater and pass through a 60-100 mesh sieve, and the mixed forage powder is obtained.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder according to the mass ratio of (1-3) to 1.
The compound vitamin is a mixture consisting of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of (4-6): (2-3): (1-3): 1.2.
The mixed forage powder comprises pasture, caulis Spatholobi, acanthopanax sessiliflorus, cowherb seed, dandelion and rehmannia, wherein the caulis Spatholobi can promote blood circulation and nourish blood, dredge tendons and activate collaterals, and the acanthopanax sessiliflorus is rich in protocatechuic acid, has good antibacterial effect, and can cause diseases such as bacterial infection, diarrhea and the like due to weak resistance of textile sows after delivery and easy corrosion of intestines and stomach by bacteria; the cowherb seed and the dandelion can help the sow to diminish inflammation and induce diuresis, and avoid urinary system infection and the like.
A large amount of fish meal, pea protein powder, seaweed meal and compound vitamins are added into the feed, so that rich protein is provided for pigs, the pigs are helped to supplement nutrition, and the milk yield and the average number of born piglets are increased.
Further preferably, the preparation method of the disease-resistant sow feed comprises the following steps:
(1) uniformly mixing 40-60 parts of mixed fermentation product, 5-10 parts of sugar/beet pulp polypeptide, 20-40 parts of mixed forage powder, 5-15 parts of mixed protein powder, 1-3 parts of composite vitamin and 15-25 parts of water according to parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the disease-resistant sow feed, wherein the grain size is 0.5-1.5 cm.
The preparation method of the sugar/beet pulp polypeptide comprises the following steps:
(1) crushing beet pulp and sieving the crushed beet pulp with a sieve of 80 to 200 meshes to obtain beet pulp powder; adding beet pulp powder into petroleum ether according to a feed-liquid ratio of 1g (5-10) mL, heating to 60-80 ℃, performing reflux extraction for 6-10h, filtering to obtain precipitate, washing and drying to obtain pretreated beet pulp; adding the pretreated beet pulp into water according to the material-liquid ratio of 1g (10-30) mL, uniformly mixing, then adding hexadecyl trimethyl ammonium bromide accounting for 0.4-0.6 wt% of the weight of the pretreated beet pulp, heating to 40-60 ℃, reacting for 20-30min, adjusting the pH to 7-10 by adopting 1mol/L sodium hydroxide aqueous solution, then adding an enzyme preparation accounting for 1-3 wt% of the weight of the pretreated beet pulp, reacting for 6-10h at 40-60 ℃, finally heating to 80-100 ℃, reacting for 5-10min, after the reaction is finished, adjusting the reaction system to be neutral, centrifuging to take supernatant, separating the supernatant by adopting a membrane ultrafiltration method, collecting polypeptide with the molecular weight of 8-15kDa, and freeze-drying to obtain the beet pulp polypeptide.
(2) Adding beet pulp polypeptide and sugar into water, uniformly mixing, heating to 80-100 ℃, reacting for 1-5h, after the reaction is finished, and freeze-drying to obtain sugar/beet pulp polypeptide, wherein the mass ratio of the beet pulp polypeptide to the sugar to the water is (1-2) to (80-100).
The enzyme preparation is one or a mixture of two or more of neutral protease, trypsin and papain.
The sugar is one or a mixture of xylo-oligosaccharide and manno-oligosaccharide. Preferably, the sugar is formed by mixing xylo-oligosaccharide and manno-oligosaccharide in a mass ratio of (1-3) to (1-3).
Beet pulp contains abundant nutrients, such as cellulose, pectin, protein, minerals, sugar, etc. The beet pulp polypeptide is prepared by further processing the beet pulp by adopting an enzyme preparation and cetyl trimethyl ammonium bromide, is easy to absorb and enter an animal circulatory system, is directly metabolized and utilized by tissues, plays an important role in animal nutrition and metabolism, can provide rich nutrition for animals, and simultaneously improves the utilization rate of protein by the animals, enhances the functions of the animals such as immunity, disease resistance and the like, thereby promoting the growth of the animals. The activity of the kinase preparation is obviously improved by the interaction of the cetyl trimethyl ammonium bromide and the enzyme preparation, the hydrolysis capacity of protein in the beet pulp is obviously improved, and the content of polypeptide in the beet pulp is further improved. The beet pulp polypeptide has certain bitter taste, and the application of the beet pulp polypeptide in the field of feed is limited. The inventor further researches and discovers that when xylooligosaccharide, mannooligosaccharide and beet pulp polypeptide are used as raw materials and are connected by covalent bonds to perform Maillard reaction to prepare the sugar/beet pulp polypeptide, fragrance is generated in the Maillard prevention process to play a food calling effect and promote the food intake of animals. Meanwhile, xylo-oligosaccharide and mannooligosaccharide in the sugar/beet pulp polypeptide are convenient for adsorbing pathogenic bacteria in intestinal tracts and regulating intestinal flora, so that the propagation of probiotics is promoted, the immune function is regulated, and the absorption of mineral elements is promoted.
The raw materials of the mixed fermentation product, the sugar/beet pulp polypeptide, the mixed forage powder, the mixed protein powder and the compound vitamin have synergistic effect and participate in the physiological activity and the metabolic regulation of organisms, so that the immunity of the organisms can be enhanced, the disease resistance rate is improved, and the growth of pigs is further promoted.
The invention has the beneficial effects that:
1. the disease-resistant sow feed prepared by the method contains the synergistic effect of the raw materials of the mixed fermentation product, the sugar/beet pulp polypeptide, the mixed forage powder, the mixed protein powder and the compound vitamin, participates in the physiological activity and the metabolic regulation of organisms, can enhance the immunity of the organisms, improve the disease resistance rate and further promote the growth of pigs. The disease-resistant sow feed contains rich nutrient substances, can effectively improve the immunity, disease resistance rate and feed intake rate of sows, and has the advantages of wide raw material source, low price and no toxic or side effect.
2. The invention takes xylo-oligosaccharide, mannooligosaccharide and beet pulp polypeptide as raw materials, takes covalent bond connection to generate Maillard reaction to prepare the sugar/beet pulp polypeptide, generates fragrance in the Maillard prevention process to play a food calling effect, and promotes the food intake of animals. Meanwhile, xylo-oligosaccharide and mannan-oligosaccharide in the sugar/beet pulp polypeptide are convenient for adsorbing pathogenic bacteria in intestinal tracts and regulating intestinal flora, so that the propagation of probiotics is promoted, the immune function is regulated, the absorption of mineral elements is promoted, and the feed intake rate and the disease resistance are obviously improved.
Detailed Description
The raw materials used in the examples were as follows:
zeolite powder, purchased from Tianjin Zeolite mining Co., Ltd, North Tibet, CAS number: 1318-02-1, particle size: 2 mm.
Vinyltriethoxysilane: coupling agent a151, purchased from carumurus facilis chemicals, inc, CAS no: 78-08-0, content: 98 percent.
Methionine, purchased from serotonine biotechnology limited, hannan, CAS no: 63-6803, content: 99 percent.
Bacillus subtilis, latin name: bacillus subtilis, number: cic 10275, effective bacterial activity: 1000 hundred million CFU/g, purchased from China center for Industrial culture Collection of microorganisms.
In the examples, the yeast is saccharomyces cerevisiae, and the name of Latin: saccharomyces cerevisiae, accession number: cic 1001, effective bacterial activity: 1000 hundred million CFU/g, purchased from China center for Industrial culture Collection of microorganisms.
Corn flour, purchased from sierra, bioengineering, ltd, cat #: REL 0941.
Sorghum flour, purchased from mingwang food ltd.
Wheat bran, purchased from kyushu chemical limited, shandong, cat # o: 781-23578.
The bean curd refuse is purchased from information services Co., Ltd, Heishan City, and has a product number of 001.
NaSeO3Purchased from north Heibo five encyclopedia, Inc., CAS number: 10102-18-8, model: food grade, content: 99 percent.
CaCl2Purchased from porch nakoco new materials technology limited, CAS No.: 10043-52-4, model: food grade, content: 98 percent.
Pasture, purchased from bihai pasture limited, zhengyang county, model: 2020.
spatholobus stem, Latin scientific name: spatholus subelectus Dunn has the effects of dispelling wind, promoting blood circulation, relaxing tendons and activating collaterals, and is purchased from Yihongtang pharmaceutical industry Co., Ltd.
Acanthopanax sessiliflorus, Latin, famous: eleutherococcus sessiliflorus (Rupr. & Maxim.) s.y.hu, has the effects of dispelling wind and eliminating dampness, invigorating stomach and inducing diuresis, and is purchased from billion hong Tang pharmaceutical industry limited company in the city of hauo.
Cowherb seed, Latin's name: the vaccine for treating amenorrhea, dysmenorrhea, galactorrhea, acute mastitis, stranguria and painful urination has the effects of activating blood circulation to promote menstruation, promoting lactation, diminishing swelling, inducing diuresis and treating stranguria, and is purchased from Yi hong Tang pharmaceutical industry limited company in the city of Hao Zhou.
Dandelion, latin scientific name: taraxacum mongolicum hand-Mazz, belonging to Compositae, contains various health nutritional components such as taraxanol, taraxacin, choline, organic acid, inulin, etc. Sweet in nature and taste, slightly bitter and cold. It enters liver and stomach meridians. Has the effects of promoting urination, relieving diarrhea, eliminating jaundice, benefiting gallbladder, etc., and is purchased from Yi Hongtong pharmaceutical industry Co Ltd.
Rehmannia, Latin scientific name: rehmannia glutinosa (Gaetn.) libosh.ex fisch.et mey, has the efficacies of nourishing yin and tonifying kidney, nourishing blood and enriching blood and cooling blood, and is purchased from a billion Hongtong pharmaceutical industry limited company in the city of Hao Zhou.
Fish meal, purchased from Ministry of agricultural feeds in Yuzhou district, Yulin.
Pea protein powder, purchased from west apres bioengineering, ltd, cat #: 5623, content 80%.
Seaweed meal, purchased from the Fufeng Sinote Biotech limited.
Beet pulp, Qinhuang island rock bomerchan Co., Ltd.
Cetyl trimethylammonium bromide, CAS: 57-09-0, bioscience, Baimuda, Nanjing.
Neutral protease, enzyme activity: 10 ten thousand u/g, CAS: 9068-59-1, Shandong Nu Ju Biotech Co., Ltd.
Trypsin, enzyme activity: 1 ten thousand u/g, CAS: 9002-07-7, Zhengzhou Yuxing food additives Co., Ltd.
Papain, enzyme activity: 10 ten thousand u/g, CAS: 9001-73-4, North Heibei Runshou Biotech Ltd.
Xylo-oligosaccharide, bio-technology ltd, zaosmund, jiangsu.
Mannooligosaccharide, food grade, Shenzhen Lefu Biotech limited.
Example 1
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to a container filled with water at 5 ℃ and quenching for 30 minutes in a water-proof manner to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent, 20 parts by weight of sulfur-containing amino acid and 6 parts by weight of yeast liquid into 50 parts by weight of water, and uniformly mixing to obtain modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the yeast liquid comprises the following steps: inoculating 2 parts by weight of yeast to 100 parts by weight of liquid medium, and culturing at 29 ℃ for 1 day to obtain yeast liquid.
The liquid medium is composed of 12 parts by weight of honey, 0.8 part by weight of glucose, 0.4 part by weight of peptone and 90 parts by weight of water.
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 2
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent, 20 parts by weight of sulfur-containing amino acid and 6 parts by weight of functionalized yeast liquid into 50 parts by weight of water, and uniformly mixing to obtain modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the functionalized yeast liquid comprises the following steps: inoculating 2 parts by weight of yeast to 100 parts by weight of liquid culture medium, and culturing at 29 ℃ for 1 day to obtain a functionalized yeast liquid;
the liquid medium comprises 12 parts of honey, 8 parts of functional agent, 0.8 part of glucose, 0.4 part of peptone and 90 parts of water in parts by weight.
The functional agent is NaSeO3。
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 3
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent, 20 parts by weight of sulfur-containing amino acid and 6 parts by weight of functionalized yeast liquid into 50 parts by weight of water, and uniformly mixing to obtain modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the functionalized yeast liquid comprises the following steps: inoculating 2 parts by weight of yeast to 100 parts by weight of liquid culture medium, and culturing at 29 ℃ for 1 day to obtain a functionalized yeast liquid;
the liquid medium comprises 12 parts of honey, 8 parts of functional agent, 0.8 part of glucose, 0.4 part of peptone and 90 parts of water in parts by weight.
The functional agent is CaCl2。
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 4
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent, 20 parts by weight of sulfur-containing amino acid and 6 parts by weight of functionalized yeast liquid into 50 parts by weight of water, and uniformly mixing to obtain modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the functionalized yeast liquid comprises the following steps: inoculating 2 parts by weight of yeast to 100 parts by weight of liquid culture medium, and culturing at 29 ℃ for 1 day to obtain a functionalized yeast liquid;
the liquid medium comprises 12 parts of honey, 8 parts of functional agent, 0.8 part of glucose, 0.4 part of peptone and 90 parts of water in parts by weight.
The functional agent is NaSeO3And CaCl2According to the mass ratio of 1: 1.8 of the composition.
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 5
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent and 20 parts by weight of sulfur-containing amino acid into 50 parts by weight of water, and uniformly mixing to obtain the modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 6
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
putting zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain modified zeolite powder;
the preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Comparative example 1
A preparation method of sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of compound vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the sow feed, wherein the particle size is 1 cm.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts by weight of zeolite powder, 0.5 part by weight of bacillus subtilis and 100 parts by weight of the crude mixture obtained in the step S1 to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the mixed forage powder comprises the following steps:
according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is prepared from fish meal, pea protein powder and seaweed powder according to a mass ratio of 2:1:1, in a mixture of the components.
The compound vitamin comprises vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2:1.2 of the composition.
Example 7
A preparation method of a disease-resistant sow feed comprises the following steps:
(1) uniformly mixing 50 parts of mixed fermentation product, 10 parts of sugar/beet pulp polypeptide, 30 parts of mixed forage powder, 10 parts of mixed protein powder, 2 parts of composite vitamin and 20 parts of water in parts by weight to obtain mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the disease-resistant sow feed, wherein the particle size is 1 cm.
The preparation method of the sugar/beet pulp polypeptide comprises the following steps:
(1) crushing beet pulp and sieving the crushed beet pulp with a 200-mesh sieve to obtain beet pulp powder; adding beet pulp powder into petroleum ether according to the feed-liquid ratio of 1g:10mL, heating at 65 ℃, performing reflux extraction for 8h, filtering to obtain precipitate, washing and drying to obtain pretreated beet pulp; adding the pretreated beet pulp into water according to the feed-liquid ratio of 1g:25mL, uniformly mixing, adding 0.4 wt% of hexadecyl trimethyl ammonium bromide based on the weight of the pretreated beet pulp, heating to 60 ℃ for reaction for 30min, adjusting the pH to 7 by using 1mol/L of sodium hydroxide aqueous solution, adding an enzyme preparation based on 2 wt% of the weight of the pretreated beet pulp, keeping the temperature at 60 ℃ for reaction for 10h, finally heating to 100 ℃ for reaction for 6min, adjusting the reaction system to be neutral after the reaction is finished, centrifuging to obtain a supernatant, separating the supernatant by using a membrane ultrafiltration method, collecting polypeptide with the molecular weight of 10kDa, and freeze-drying to obtain the beet pulp polypeptide.
(2) Adding beet pulp polypeptide and sugar into water, uniformly mixing, heating to 85 ℃, reacting for 3h, after the reaction is finished, and freeze-drying to obtain sugar/beet pulp polypeptide, wherein the mass ratio of the beet pulp polypeptide to the sugar to the water is 1:1: 80.
The enzyme preparation is prepared by mixing neutral protease, trypsin and papain in a mass ratio of 1:1: 1.
The sugar is prepared by mixing xylo-oligosaccharide and mannan-oligosaccharide according to the mass ratio of 3: 1.
The preparation method of the mixed fermentation product comprises the following steps:
s1: uniformly mixing 40 parts of corn flour, 40 parts of sorghum flour, 20 parts of wheat bran, 15 parts of bean curd residue and 40 parts of water in parts by weight to obtain a crude mixture;
s2: uniformly mixing 18 parts of modified zeolite powder, 0.5 part of bacillus subtilis and 100 parts of the crude mixture obtained in the step S1 according to parts by weight to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 36 hours at 25 ℃ to obtain a mixed fermentation product.
The preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 420 ℃ at an initial temperature of 25 ℃ at a heating rate of 5 ℃/min, keeping for 2 hours, then cooling to 80 ℃ at a cooling rate of 10 ℃/min, transferring to 5 ℃ and keeping for 30 minutes to obtain physically modified zeolite powder;
t2: adding 20 parts by weight of the physically modified zeolite powder obtained in the step T1 into 50 parts by weight of the modification solution, uniformly mixing, soaking at 60 ℃ for 3 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (3) transferring the mixture A obtained in the step T2 to an oven at 60 ℃ for drying for 10 hours to obtain the modified zeolite powder.
The preparation method of the modified liquid comprises the following steps: adding 5 parts by weight of silane coupling agent, 20 parts by weight of sulfur-containing amino acid and 6 parts by weight of functionalized yeast liquid into 50 parts by weight of water, and uniformly mixing to obtain modified liquid.
The silane coupling agent is vinyl triethoxysilane.
The sulfur-containing amino acid is methionine.
The preparation method of the functionalized yeast liquid comprises the following steps: inoculating 2 parts by weight of yeast to 100 parts by weight of liquid culture medium, and culturing at 29 ℃ for 1 day to obtain a functionalized yeast liquid;
the liquid medium comprises 12 parts of honey, 8 parts of functional agent, 0.8 part of glucose, 0.4 part of peptone and 90 parts of water in parts by weight.
The functional agent is NaSeO3And CaCl2According to the mass ratio of 1: 1.8 of the composition.
The preparation method of the mixed forage powder comprises the following steps: according to the weight parts, 20 parts of pasture, 5 parts of caulis spatholobi, 5 parts of acanthopanax sessiliflorus, 5 parts of cowherb seed, 10 parts of dandelion and 5 parts of rehmannia are crushed by a beater and pass through a 80-mesh sieve to obtain mixed forage powder.
The mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of 2:1: 1.
The compound vitamin is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 according to the mass ratio of 5:2.3:2: 1.2.
Example 8
Essentially the same as example 7, except that: the beet pulp polypeptide is adopted to replace sugar/beet pulp polypeptide.
The preparation method of the beet pulp polypeptide comprises the following steps:
crushing beet pulp and sieving the crushed beet pulp with a 200-mesh sieve to obtain beet pulp powder; adding beet pulp powder into petroleum ether according to the feed-liquid ratio of 1g:10mL, heating at 65 ℃, performing reflux extraction for 8h, filtering to obtain precipitate, washing and drying to obtain pretreated beet pulp; adding the pretreated beet pulp into water according to the feed-liquid ratio of 1g:25mL, uniformly mixing, adding 0.4 wt% of hexadecyl trimethyl ammonium bromide based on the weight of the pretreated beet pulp, heating to 60 ℃ for reaction for 30min, adjusting the pH to 7 by using 1mol/L of sodium hydroxide aqueous solution, adding an enzyme preparation based on 2 wt% of the weight of the pretreated beet pulp, keeping the temperature at 60 ℃ for reaction for 10h, finally heating to 100 ℃ for reaction for 6min, adjusting the reaction system to be neutral after the reaction is finished, centrifuging to obtain a supernatant, separating the supernatant by using a membrane ultrafiltration method, collecting polypeptide with the molecular weight of 10kDa, and freeze-drying to obtain the beet pulp polypeptide.
The enzyme preparation is prepared by mixing neutral protease, trypsin and papain in a mass ratio of 1:1: 1.
Test example 1
The sow feed in the examples and the comparative examples is subjected to a sanitation index test, the test standard adopts GB 13078-.
Table 1: hygiene index test
Figure BDA0003374862530000201
The zeolite powder is a molecular sieve with honeycomb-like crystals, can adsorb low molecular compounds, toxic compounds, mycotoxin and the like, adsorbs heavy metals through cation exchange characteristics, and is discharged out of a body, and has certain antibacterial performance and heavy metal adsorption performance, and toxins and heavy metals are discharged out of the body along with the metabolism of the body. After the zeolite powder is modified, the pore structure is more stable, the comparative area is larger, sulfur-containing amino acid is fixed on the surface of the zeolite powder, the silicon content on the surface of the zeolite powder is reduced, the porosity is improved, the average crystal and particle size of the silicon-containing zeolite powder material are reduced, and the antibacterial property, adsorbability and cation exchange property of the zeolite powder are further enhanced.
Test example 2
The sow feed in the examples and the comparative example is subjected to a sow lactation performance test, 180 sows which are just produced are selected, randomly divided into 9 groups, and each group has 20 sows, and the sows are fed by the sow feed in the examples and the comparative example respectively, and feeding management is carried out according to the daily management rules of a pig farm.
Wherein: average daily lactation yield of sows (total milk yield of sows in 2-21 days after production) is 400; the average diarrhea rate of the sows is 400 per 2-21 days of postnatal diarrhea of each group of sows; the average incidence rate of milk fever of the sows is the total frequency/400 of the milk fever diseases of each group of sows occurring within the 2 nd to 21 th days after delivery, and the test results are shown in the table 2.
Table 2: sow lactation performance test
Figure BDA0003374862530000211
The modified zeolite powder has a honeycomb network crystal structure, enters a pig body through an oral cavity, is absorbed by intestinal mucosa and enters blood and lymph tissue, has higher specific surface area and adsorption capacity, can adsorb microorganisms such as low molecular compounds, toxic compounds, mycotoxin and the like in the body, dissolves some small molecular substances and discharges the small molecular substances out of the body; the modified zeolite powder can be used as an adsorbent of heavy metals to adsorb lead, chromium, copper and the like through cation exchange characteristics, and the antibacterial and anti-diarrhea functions of the sow feed added with the modified zeolite powder are obviously enhanced;
the selenium-rich yeast and the calcium-rich yeast are introduced with sulfur-containing amino acid to modify the zeolite powder, selenium and calcium in the yeast are fixed on the amino acid on the surface of the zeolite powder, and the zeolite powder is further modified to improve the porosity, adsorption capacity and ion exchange performance of the zeolite powder. The modified zeolite powder and the generated sodium bisulfite can be used for chemical detoxification of mycotoxin in sow feed, can effectively reduce the harm of joint protection mycotoxin to pig intestines, keep healthy intestines and obviously enhance the diarrhea resistance function; after the delivery of the sow, the requirement of the produced milk on calcium is suddenly increased, the secretion of the parathyroid hormone and the calcitonin cannot adapt to the sudden change, postpartum paralysis is caused when the sow is lack of calcium, namely, the symptoms of milk fever are caused, the sow feed added with the calcium-rich yeast and the modified zeolite powder can improve the resistance of the sow and also can participate in adjusting the calcium stable matrix of the postpartum sow, the ash content, the calcium content and the phosphorus content and the strength of the coelomic bone are increased, and the generation of the milk fever is avoided and the milk secretion quality is improved by adjusting the calcium content in the serum.

Claims (10)

1. The preparation method of the disease-resistant sow feed is characterized by comprising the following steps:
(1) uniformly mixing the mixed fermentation product, the sugar/beet pulp polypeptide, the mixed forage powder, the mixed protein powder, the compound vitamin and the water to obtain a mixed feed;
(2) and (2) placing the mixed feed obtained in the step (1) into a feed granulator for granulation to obtain the disease-resistant sow feed.
2. The method for preparing the disease-resistant sow feed as claimed in claim 1, wherein the method for preparing the sugar/beet pulp polypeptide comprises the following steps:
(1) crushing beet pulp and sieving the crushed beet pulp with a sieve of 80 to 200 meshes to obtain beet pulp powder; adding beet pulp powder into petroleum ether according to a feed-liquid ratio of 1g (5-10) mL, heating to 60-80 ℃, performing reflux extraction for 6-10h, filtering to obtain precipitate, washing and drying to obtain pretreated beet pulp; adding the pretreated beet pulp into water according to the material-liquid ratio of 1g (10-30) mL, uniformly mixing, then adding hexadecyl trimethyl ammonium bromide accounting for 0.4-0.6 wt% of the weight of the pretreated beet pulp, heating to 40-60 ℃, reacting for 20-30min, adjusting the pH to 7-10 by adopting 1mol/L sodium hydroxide aqueous solution, then adding an enzyme preparation accounting for 1-3 wt% of the weight of the pretreated beet pulp, reacting for 6-10h at 40-60 ℃, finally heating to 80-100 ℃, reacting for 5-10min, after the reaction is finished, adjusting the reaction system to be neutral, centrifuging to take supernatant, separating the supernatant by adopting a membrane ultrafiltration method, collecting polypeptide with the molecular weight of 8-15kDa, and freeze-drying to obtain the beet pulp polypeptide;
(2) adding beet pulp polypeptide and sugar into water, uniformly mixing, heating to 80-100 ℃, reacting for 1-5h, after the reaction is finished, and freeze-drying to obtain sugar/beet pulp polypeptide, wherein the mass ratio of the beet pulp polypeptide to the sugar to the water is (1-2) to (80-100).
3. The method for preparing the disease-resistant sow feed as claimed in claim 1, wherein the method for preparing the mixed fermentation product comprises the following steps:
s1: uniformly mixing 30-50 parts of corn flour, 30-50 parts of sorghum flour, 15-25 parts of wheat bran, 10-20 parts of bean curd residue and 30-50 parts of water in parts by weight to obtain a coarse mixture;
s2: uniformly mixing 8-20 parts by weight of modified zeolite powder, 0.4-0.6 part by weight of bacillus subtilis and 80-120 parts by weight of the crude mixture obtained in the step S1 to obtain a fermentation substrate;
s3: and (4) placing the fermentation substrate obtained in the step (S2) into a fermentation tank, and sealing and fermenting for 30-40 hours at 23-27 ℃ to obtain a mixed fermentation product.
4. The preparation method of the disease-resistant sow feed as claimed in claim 3, wherein the preparation method of the modified zeolite powder comprises the following steps:
t1: putting the zeolite powder into a tubular furnace, heating to 400-420 ℃ at the initial temperature of 20-30 ℃ at the heating rate of 4-6 ℃/min, keeping for 1-3 hours, then cooling to 70-90 ℃ at the cooling rate of 8-12 ℃/min, transferring to 4-6 ℃ and keeping for 20-40 minutes to obtain physically modified zeolite powder;
t2: adding 15-25 parts by weight of the physically modified zeolite powder obtained in the step T1 into 40-60 parts by weight of the modification solution, uniformly mixing, soaking at 50-70 ℃ for 2-4 hours, and filtering to obtain a filter cake to obtain a mixture A;
t3: and (4) transferring the mixture A obtained in the step T2 to an oven at 50-70 ℃ for drying for 8-12 hours to obtain the modified zeolite powder.
5. The method for preparing the disease-resistant sow feed as claimed in claim 4, wherein the method for preparing the modified liquid comprises the following steps: adding 4-6 parts by weight of silane coupling agent, 15-25 parts by weight of sulfur-containing amino acid and 5-7 parts by weight of functional yeast liquid into 40-60 parts by weight of water, and uniformly mixing to obtain the modified liquid.
6. The method for preparing the disease-resistant sow feed as claimed in claim 5, wherein the method for preparing the functionalized yeast liquid comprises the following steps: according to the weight portion, 1-2 portions of yeast are inoculated on 90-110 portions of liquid culture medium, and cultured for 1-2 days at 28-30 ℃ to obtain the functionalized yeast liquid.
7. The method for preparing the disease-resistant sow feed as claimed in claim 1, wherein the method for preparing the mixed forage comprises the following steps: according to the weight portion, 10-30 portions of pasture, 3-7 portions of caulis spatholobi, 3-7 portions of acanthopanax sessiliflorus, 3-7 portions of cowherb seed, 8-12 portions of dandelion and 3-7 portions of rehmannia are crushed by a beater and pass through a 60-100 mesh sieve, and the mixed forage powder is obtained.
8. The method for preparing the disease-resistant sow feed as claimed in claim 1, wherein the mixed protein powder is a mixture of fish meal, pea protein powder and seaweed powder in a mass ratio of (1-3):1.
9. The method for preparing the disease-resistant sow feed as claimed in claim 1, wherein the vitamin complex is a mixture of vitamin C, vitamin E, vitamin B1 and vitamin B6 in a mass ratio of (4-6): 2-3): 1-3): 1.2.
10. A disease-resistant sow feed prepared by the method of any one of claims 1-9.
CN202111412811.9A 2021-11-25 2021-11-25 Disease-resistant sow feed and preparation method thereof Withdrawn CN114009615A (en)

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