CN106562059A - Lactating sow feed and preparation method thereof - Google Patents
Lactating sow feed and preparation method thereof Download PDFInfo
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- CN106562059A CN106562059A CN201610969209.8A CN201610969209A CN106562059A CN 106562059 A CN106562059 A CN 106562059A CN 201610969209 A CN201610969209 A CN 201610969209A CN 106562059 A CN106562059 A CN 106562059A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
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- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention discloses a lactating sow feed and belongs to the technical field of animal feed. The lactating sow feed comprises fermented broussonetia papyrifera leaf powder, corns, brown rice, extruded soybeans, beet pulp, flaxseeds, soybean hull, alfalfa meal, cane molasses, calcium hydrophosphate, mountain flour, phytase, salt, lysine, methionine, threonine, choline chloride, mildew preventive, microecologics, a compound premixed feed and antioxidant. The fabrication method includes the steps of preliminary screening, crushing, compounding, feeding, mixing, pelletizing and the like. The coarse fiber content of fermented broussonetia papyrifera leaves is low, the coarse protein content is increased, amino acid is more complete, palatability is greatly improved, and the mixed feed fabricated by matching the fermented broussonetia papyrifera leaves with other raw materials can increase food intake of sows, improve the constipation condition of the sows, improve growth performance of suckling pigs and thus increase pig farm production benefits.
Description
Technical field
The invention belongs to technical field of animal, and in particular to a kind of agent of feed for nursing sow and preparation method thereof.
Background technology
Sow is the electromotor on a pig farm, and the reproductive performance height of sow determines a pig farm production in a way
The quality of index, the reproductive performance of prevention of sow constipation meeting extreme influence sow, shows as：Reduce feed intake, duration of labor extend, it is dead
Tire several litres are high, induction MMA (mastitiss-metritiss-agalactia syndrome), reduce lactation amount, wean son's number living and reduce etc..And,
Sow milk Aquatic product amount and quality, determine the speed of growth and body constitution of pigletss.So solving prevention of sow constipation and improving sow galactopoiesis
Performance is to lift sow production performance, and then improves the premise of whole pig farm production target.
Broussonetia papyrifera original name paper mulberry, is Moraceae structure platymiscium, and growing power is extremely strong, and Broussonetia papyrifera is barren-resistant, drought-resistant, few pest and disease damage, branch
It is leafiness, big and fleshy, spread all over the gully of China mountain area, hilly country, aboundresources.Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., sweet in the mouth cool in nature, removing heat from blood, diuretic.Control
Haematemesis, metrorrhagia, traumatic hemorrhage, edema, hernia, dysentery, tinea skin ulcer.Simultaneously leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. contains abundant aminoacid, crude protein, micro
Element etc..It is nutritious in the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of air bells dry basis, wherein protein content up to 17～29%, crude fiber content 12～
20%, crude fat 5～12%, calcium 2～5%, phosphorus 0.4～2%.The nutrient substance enriched in leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. can meet sow to albumen
The demand of matter, vitamin, crude fibre and calcium phosphorus etc..The crude fibre enriched especially in leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. and trace element and its property of medicine are to changing
Kind prevention of sow constipation has good effect, and then improves suckling pig growth performance.Meanwhile, Broussonetia papyrifera is also weight of the country as poverty alleviation
Point project, using leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. as feedstuff, is both a new way of protein feed resources exploitation, and the one of increasing peasant income
Item source, is to kill two birds with one stone.But, due to crude fibre higher in leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. and poor palatability, which is limited in pig
Consumption in the monogastric animal feeds such as fowl.
The content of the invention
In order to solve the above problems, it is an object of the invention to provide a kind of agent of feed for nursing sow and preparation method thereof.
For achieving the above object, the technical solution used in the present invention is as follows：
A kind of agent of feed for nursing sow, the raw material containing following weight portion：Fermented papermulberry tree powder 5-15 parts, Semen Maydiss 20-50 parts,
It is brown rice 5-10 parts, puffing Semen sojae atricolor 4-6 parts, beet pulp 3-4 parts, Semen Lini 2-3 parts, soybean cover 3-5 part, alfalfa meal 3-4 parts, sweet
Cane molasses 2-3 parts, calcium hydrogen phosphate 0.8-1.2 parts, stone powder 0.8-1.2 parts, high temperature resistant phytase 0.01-0.03 parts, Tiny ecosystem system
Agent 0.01-0.04 parts, Sal 0.3-0.5 parts, lysine 0.2-0.6 parts, threonine 0.06-0.1 parts, methionine 0.05-0.15
Part, choline chloride 0.1-0.2 parts, antifungus agent 0.05-0.25 parts, compound premixed feed 0.2-0.6 parts and antioxidant 0.02-
0.07 part.
In one embodiment of the invention, described agent of feed for nursing sow, the raw material containing following weight portion：Fermentation structure
10 parts of leaf powder, 40.3 parts of Semen Maydiss, 6.5 parts of brown rice, 18 parts of bean cake, 5.5 parts of puffing Semen sojae atricolor, 3.5 parts of beet pulp, Semen Lini 3.22
Part, 3.8 parts of soybean cover, 4 parts of alfalfa meal, 2 parts of cane molasses, 0.86 part of calcium hydrogen phosphate, 0.84 part of stone powder, high temperature resistant phytase
0.01 part, 0.41 part of Sal, 0.03 part of microbial ecological agent, 0.33 part of lysine, 0.1 part of threonine, 0.04 part of methionine, chlorination
0.15 part of choline, 0.1 part of antifungus agent, 0.25 part of compound premixed feed, 0.06 part of antioxidant.
Wherein, described fermented papermulberry tree powder is prepared as follows：The leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing and 10% mass concentration
Glucose solution mixing after, add aspergillus niger and bacillus subtilises mixed bacteria liquid, after stirring, control material water content
For 40～50%, constant temperature koji tray solid fermentation carries out drying and crushing after fermentation ends.
Wherein, the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder and the mixed proportion of glucose solution are mass ratio 2:1.
Wherein, by aspergillus niger bacterium solution (1 × 106～7Individual/mL) and bacillus subtilises bacterium solution (1 × 107～8Individual/mL) by matter
Amount is than being 2:1 ratio is mixed into mixed bacteria liquid, and mixed bacteria liquid inoculum concentration is 10%～15%.
Wherein, the viable count of the aspergillus niger bacterium solution is 1 × 106～7CFU/mL, the viable bacteria of the bacillus subtilises bacterium solution
Number is, 1 × 107～8CFU/mL。
Wherein, fermentation temperature is 30～37 DEG C, and fermentation time is 4～8 days.
Wherein, the compound micro-ecological preparation is made up of probiotic bacteria and adjuvant, and the probiotic bacteria is by Lactobacillus plantarum
(lactobacillus plantarum) CGMCC No.11262, bacillus subtilises (Bacillus subtilis) CGMCC
No.11261 and Bacillus pumilus (Bacillus pumilus) CGMCC No.12197 compositions, every gram of compound micro-ecological preparation
In, the viable count of Lactobacillus plantarum (lactobacillus plantarum) CGMCC No.11262 is 75-100 hundred million, hay bud
The viable count of spore bacillus (Bacillus subtilis) CGMCC No.11261 be 25-75 hundred million, Bacillus pumilus
The viable count of (Bacillus pumilus) CGMCC No.12197 is 75-125 hundred million.
The mycopowder of each probiotic bacteria is prepared respectively by the conventional method in this area, is then mixed.
The Lactobacillus plantarum (Lactobacillus plantarum) of the present invention separates multiple through primary dcreening operation from piglet feces
Sieve is obtained, and the colonial morphology in MRS culture medium is bacterium colony pale, circular, moistening, and droplet-like is raised, and surface is smooth, has
Gloss, neat in edge are opaque.Thalline is shaft-like, it is single or in short chain arrangement exist, G+ bacteria strain, without spore.
It is initially identified as Lactobacillus plantarum.
Identify after 16s rDNA PCR amplifications are carried out to the bacterial strain for filtering out using bacterial universal primers, Jing NCBI sequence ratios
To result similarity highest (100%) is Lactobacillus plantarum, therefore Molecular Identification DZS12 is Lactobacillus plantarum.
It is Lactobacillus plantarum DZS12 (lactobacillus plantarum DZS12) by the Strain Designation, in 2015
August is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, abbreviation CGMCC, address on the 20th：Beijing
No. 3 Institute of Microorganism, Academia Sinica of institute of Chaoyang District North Star West Road 1, deposit number is：CGMCC No.11262.
By extracorporeal bacteria inhibitor test, growth of the bacterial strain to E.coli K88, e. coli k99 and Salmonella has
Effect is significantly inhibited, preferably, its bacteriostasis rate to E.coli K88 reaches 85.34% to antibacterial resultant effect；To escherichia coli
The bacteriostasis rate of K99 reaches 81.59%；75.56% is reached to the bacteriostasis rate of Salmonella simultaneously.
The bacillus subtilises of the present invention separate from piglet feces and obtain through primary dcreening operation secondary screening.The bacterial strain is acidproof, resistance to gallbladder
Salt, with very high enzymatic productivity.Quantitative determine through amylase activity, the amylase activity of the bacterial strain is 3285.87U.Jing
Prolease activity quantitative determination is crossed, the neutral protease vigor of the bacterial strain is 65.9U, and acid protease activity is 41.6U.Pass through
Zoopery finds which can effectively facilitate the production performance of poultry.It is bacillus subtilises DZS11 by the Strain Designation
(Bacillus subtilis DZS11), is preserved in China Committee for Culture Collection of Microorganisms on 20th in August in 2015 general
Logical microorganism center, abbreviation CGMCC, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese Academy of Sciences microbe research
Institute, deposit number is：CGMCC No.11261.
The present invention is separated from tilapia Cultivated water, oriented primary dcreening operation and secondary screening obtain a bacillus pumilus.Its
Trophocyte thin rod shape, its spore are oval or long tubular, are that middle life or secondary end are given birth to, and sporangiocyst slightly expands.Isolate or in short chain, bar
End is semicircle.Bacterium colony can be formed in 12～16h, bacterium colony is circle, or irregularly, edge is hair-like, and bacteria colony white is impermeable
It is bright, corrugationless.Gram-positive.
Bacillus pumilus to obtaining have carried out biocidal property, heat resistance, fermentation character experiment.In flat board test process
In, it is found that the bacterial strain can significantly inhibit the growth of streptococcus agalactiae, form significantly transparent suppression circle.The bacterial strain spore temperature tolerance
Can be good, spore is in 85 DEG C of water-bath 10min, spore survival rate more than 80%.
The research of early stage finds that the bacterial strain is can apply in aquatic feeds, improves resistivity of the Fish to streptococcicosises.
By carrying out extracorporeal bacteria inhibitor test discovery to the bacterial strain again, the bacterial strain also can effectively suppress escherichia coli, Staphylococcus aureus
Bacterium and Salmonella, reach 86.57% to the bacteriostasis rate of E.coli K88；The bacteriostasis rate of e. coli k99 is reached
84.28%；82.14% is reached to the bacteriostasis rate of staphylococcus aureuses, 79.68% is reached to the bacteriostasis rate of Salmonella.Together
When, being determined by external cellulase activity, the cellulase activity of the bacterial strain has reached 1692.13U/mL, is very beneficial for mother
Pig digests and assimilates the cellulose in feedstuff.
The Bacillus pumilus for screening (Bacillus pumilus) are preserved in into Chinese micro- life on March 9th, 2016
Thing culture presevation administration committee common micro-organisms center, address：Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 Chinese science
Institute of microbiology of institute, deposit number is：CGMCC No.12197.
Wherein, contain in per kilogram of compound premixed feed：Vitamin A 0.08-0.28g, vitamin D3
0.024-0.1424g, Vitamin E 1.3-7mg, vitamin K33.36-4.8g, vitamin B12-3.5g, vitamin B212-
14g, vitamin B67-9g, vitamin B1238-46mg, nicotiamide 56-67g, D-VB5 calcium 30-42g, Folic Acid 10.5-
12.4g, biotin 1.05-2.5g, copper sulfate 3-12.5g, ferrous sulfate 30-165g, zinc sulfate 50-100g, manganese sulfate monohydrate
25-85g, sodium selenite 65-320mg, calcium iodate 0.5-5g, balance of carrier powdered rice hulls.
Wherein, the antioxidant includes ethoxy quinoline, dibenzylatiooluene, Butylated hydroxyanisole, Galla Turcica (Galla Helepensis)
Two or more in acid.
Wherein, the antifungus agent include propanoic acid, calcium propionate, formic acid, calcium formate, sodium propionate, benzoic acid or sorbic acid and its
Salt apoplexy due to endogenous wind one or two.
In order to preferably realize foregoing invention purpose, present invention also offers a kind of making side of breastfeeding sow compound feed
Method, the manufacture method include：
By Semen Maydiss, bean cake is crushed powdering with the sieve of 3.0mm, enters and treat batch bin through primary dcreening operation process, after crushing
Semen Maydiss, brown rice, bean cake, beet pulp, Semen Lini, soybean cover and fermented papermulberry tree powder, puffing Semen sojae atricolor and alfalfa meal are by big
Material burden balance are proportionally added into mixer, then respectively by calcium hydrogen phosphate, stone powder, Sal, lysine, methionine, threonine, multiple
Close premixed feed, choline chloride, phytase, compound micro-ecological preparation, antifungus agent, antioxidant to weigh from micro-balance or manually
Mixer is added, and is pelletized into storehouse to be pelletized after cane molasses mix homogeneously being added after the mixing 10-15 seconds.
In one embodiment of the invention, grinding particle size 3.0-4.0mm is equal through the incorporation time mixing of 90-150 seconds
It is even, to be pelletized under conditions of vapour pressure 0.2-0.25Mpa, temperature 60-85 DEG C, particle diameter is 3.5-4.0mm, and cool time is
≥5min。
Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. crude protein content 20.13%, crude fiber content 14.32%, lysine content 1.26% used by of the invention.
After leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. is fermented, crude protein content 23.15～26.30% improves 15～30.65%；Crude fiber content be 8.65～
11.78%, reduce 17.74～39.59%；Lysine content is 1.78～2.07%, improves 41.27～64.29%, battalion
Foster value is substantially improved, while palatability is also greatly improved.
After leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. is fermented by the present invention, crude fiber content is reduced, increases crude protein content, make aminoacid more comprehensive,
Its palatability is substantially improved, the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. after fermentation solves prevention of sow constipation feelings with the latter made mixed feed of other material matchings
Condition, improves sow milk water quality and yield, improves piglet growth performance.
The agent of feed for nursing sow of the present invention meeting the nutritional need of nursing sow while, substantially make use of fermentation structure
Leaveves.Using the enough dietary fiber nutrients of beet pulp, soybean cover, alfalfa meal and fermented papermulberry tree guarantee feedstuff and reasonably
Coarse fiber structure, increases sow satiety, promotes gastrointestinal motility, effectively prevents stiff sow feces, gastric ulcer and mechanical row
For generation, meanwhile, do not use the crude fibre raw material of the such easy infection mycete of wheat bran, make sow material safer；Addition cane suger
Honey improves the palatability of sow mixed feed, increases sow feed intake, and the magnesium salt in cane molasses has the gently effect of letting out, effectively
Prevent prevention of sow constipation；Addition puffing Semen sojae atricolor and Semen Lini, central unsaturated fatty acid is to sow development of breast and fetal growth
Development is with good facilitation；Present invention addition compound micro-ecological preparation, with excellent synergy, adjusts intestinal jointly
Road Bacterial community, keeps microecological balance, probiotic bacteria and its metabolite promote digesting and assimilating for nutrient substance, improves and raise
The utilization rate of material, improves the milk yield and milk water quality of sow, so as to improve the production performance of piglet.
Specific embodiment
By the following example by the more specific description present invention, it should be understood that the embodiment is merely to illustrate this
Invention, rather than the scope of the present invention is limited by any way.
The preparation of 1 fermented papermulberry tree powder of embodiment
(1) prepared by aspergillus niger and bacillus subtilises mixed bacteria liquid：
It is prepared by Aspergillus niger spores suspension：
It is prepared by seed culture medium (PDA)：Distilled water 1000mL, Rhizoma Solani tuber osi leach powder 6g, glucose 20g, agar 16g, 115
DEG C sterilizing 30min；
It is prepared by strain：The mycete for having activated is accessed in PDA culture medium with inoculating loop, constant temperature culture 3-4d at 30 DEG C is treated
Spore is ripe；
It is prepared by spore suspension：Spore is eluted with appropriate sterilized water, is inserted in the shaking flask equipped with bead,
140r/min levels shake 1h, after menses ball plate is counted, make concentration for 1 × 106～7The spore suspension of CFU/mL.
It is prepared by bacillus subtilises bacterium solution：Appropriate steaming is dissolved according to 3g/L Carnis Bovis seu Bubali creams, 10g/L peptones and 5g/L Sodium Chloride
In distilled water, 121 DEG C of sterilizing 30min.The bacillus subtilis liquid 2ml that access has been activated, shaken cultivation 24 hours.Menses ball plate is counted
Afterwards, concentration is made for 1 × 107～8CFU/mL。
By the Aspergillus niger spores suspension for preparing with bacillus subtilis bacterium solution according to mass ratio (2:1) mix.
(2) mixed fermentation：
By the G/W of the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing and 10% mass concentration according to (W:W=2：1) mix after, respectively plus
Enter 10% Aspergillus niger spores suspension and bacillus subtilises mixed bacteria liquid, it is after stirring, under 30 DEG C of constant temperature, bent
Disk solid fermentation 8 days.
(3) drying and crushing detection：
Drying and crushing is carried out after fermentation ends, 40 mesh sieves are crossed.
Before fermentation, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is detected：Crude protein content 20.13%, crude fiber content 14.32%, lysine content
1.26%.In fermented papermulberry tree powder：Crude protein content 23.15%, crude fiber content 11.78%, lysine 1.78%.
The preparation of 2 fermented papermulberry tree powder of embodiment
(1) prepared by aspergillus niger and bacillus subtilises mixed bacteria liquid：With embodiment 1
(2) mixed fermentation：
By the G/W of the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing and 10% mass concentration according to (W:W=2：1) mix after, respectively plus
Enter 12% aspergillus niger and bacillus subtilises mixed bacteria liquid, after stirring, under 32 DEG C of constant temperature, koji tray solid fermentation
6 days.
(3) drying and crushing detection：
Drying and crushing is carried out after fermentation ends, 40 mesh sieves are crossed.
Before fermentation, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is detected：Crude protein content 20.13%, crude fiber content 14.32%, lysine content
1.26%.In fermented papermulberry tree powder：Crude protein content 24.75%, crude fiber content 9.71%, lysine 2.02%.
The preparation of 3 fermented papermulberry tree powder of embodiment
(1) prepared by aspergillus niger and bacillus subtilises mixed bacteria liquid：With embodiment 1
(2) mixed fermentation：
By the G/W of the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing and 10% mass concentration according to (W:W=2：1) mix after, respectively plus
Enter 15% aspergillus niger and bacillus subtilises mixed bacteria liquid, after stirring, under 35 DEG C of constant temperature, koji tray solid fermentation
5 days.
(3) drying and crushing detection：
Drying and crushing is carried out after fermentation ends, 40 mesh sieves are crossed.
Before fermentation, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is detected：Crude protein content 20.13%, crude fiber content 14.32%, lysine content
1.26%.After testing in fermented papermulberry tree powder：Crude protein content 23.95%, crude fiber content 9.86%, lysine 2.07%.
The preparation of 4 fermented papermulberry tree powder of embodiment
(1) prepared by aspergillus niger and bacillus subtilises mixed bacteria liquid：With embodiment 1
(2) mixed fermentation：
By the G/W of the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing and 10% mass concentration according to (W:W=2：1) mix after, respectively plus
Enter 15% aspergillus niger and bacillus subtilises mixed bacteria liquid, after stirring, under 36 DEG C of constant temperature, koji tray solid fermentation
6 days.
(3) drying and crushing detection：
Drying and crushing is carried out after fermentation ends, 40 mesh sieves are crossed.
Before fermentation, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is detected：Crude protein content 20.13%, crude fiber content 14.32%, lysine content
1.26%.After testing in fermented papermulberry tree powder：Crude protein content 26.3%, crude fiber content 8.65%, lysine 1.96%.
By comparing, the fermented papermulberry tree index of embodiment 4 is even more ideal, therefore selects the fermented papermulberry tree powder of embodiment 4
Prepare breastfeeding sow compound feed.
The preparation of 5 breastfeeding sow compound feed of embodiment
Following component is prepared by batch weight part：5 parts of fermented papermulberry tree powder, 43.3 parts of Semen Maydiss, 6.5 parts of brown rice, bean cake
20 parts, 5.5 parts of puffing Semen sojae atricolor, 3.5 parts of beet pulp, 3.22 parts of Semen Lini, 3.8 parts of soybean cover, 4 parts of alfalfa meal, cane molasses 2
Part, 0.86 part of calcium hydrogen phosphate, 0.84 part of stone powder, 0.01 part of high temperature resistant phytase, 0.41 part of Sal, 0.03 part of microbial ecological agent,
0.33 part of lysine, 0.1 part of threonine, 0.04 part of methionine, 0.15 part of choline chloride, 0.1 part of antifungus agent, compound premixing are closed raises
0.25 part of material, 0.06 part of antioxidant are made.
By Semen Maydiss, bean cake after primary dcreening operation process is crushed powdering with the sieve of 3.0mm, enters and treat batch bin, will crush
Semen Maydiss afterwards, brown rice, bean cake, beet pulp, Semen Lini, soybean cover and fermented papermulberry tree powder, puffing Semen sojae atricolor and alfalfa meal pass through
Fructus Anisi Stellati burden balance are proportionally added into mixer, then respectively by calcium hydrogen phosphate, stone powder, Sal, lysine, methionine, threonine,
Compound premixed feed, choline chloride, phytase, compound micro-ecological preparation, antifungus agent, antioxidant claim from micro-balance or manually
After amount adds mixer, mixing cane molasses mixing to be added after 15 seconds 120 seconds, in vapour pressure 0.2Mpa, the bar that 80 DEG C of temperature
Pelletized under part, particle diameter 3.5mm, cool time are 10min.
The preparation of 6 breastfeeding sow compound feed of embodiment
Following component is prepared by batch weight part：10 parts of fermented papermulberry tree powder, 40.3 parts of Semen Maydiss, 6.5 parts of brown rice, bean cake
18 parts, 5.5 parts of puffing Semen sojae atricolor, 3.5 parts of beet pulp, 3.22 parts of Semen Lini, 3.8 parts of soybean cover, 4 parts of alfalfa meal, cane molasses 2
Part, 0.86 part of calcium hydrogen phosphate, 0.84 part of stone powder, 0.01 part of high temperature resistant phytase, 0.41 part of Sal, 0.03 part of microbial ecological agent,
0.33 part of lysine, 0.1 part of threonine, 0.04 part of methionine, 0.15 part of choline chloride, 0.1 part of antifungus agent, compound premixing are closed raises
0.25 part of material, 0.06 part of antioxidant are made.
Preparation method is with embodiment 5.
The preparation of 7 breastfeeding sow compound feed of embodiment
Following component is prepared by batch weight part：15 parts of fermented papermulberry tree powder, 38.3 parts of Semen Maydiss, 6.5 parts of brown rice, bean cake
16.5 parts, 5.5 parts of puffing Semen sojae atricolor, 3 parts of beet pulp, 3.22 parts of Semen Lini, 3.3 parts of soybean cover, 4 parts of alfalfa meal, cane molasses 2
Part, 0.86 part of calcium hydrogen phosphate, 0.84 part of stone powder, 0.01 part of high temperature resistant phytase, 0.41 part of Sal, 0.03 part of microbial ecological agent,
0.33 part of lysine, 0.1 part of threonine, 0.04 part of methionine, 0.15 part of choline chloride, 0.1 part of antifungus agent, compound premixing are closed raises
0.25 part of material, 0.06 part of antioxidant are made.
Preparation method is with embodiment 5.
The screening of 1 leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. fermented bacterium of test example
Using each single or composite bacteria as fermented bacterium, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder and mass concentration 10% G/W (W:W=
2:1) it is fermentation substrate, at 32 DEG C, after 5.5 days, dries pulverizing is detected, as a result as shown in table 1 koji tray solid fermentation.
The screening of 1 fermented bacterium of table
As known from Table 1, aspergillus niger+bacillus subtilises complex group, ferment effect are optimal.
Impact of the breastfeeding sow compound feed that 2 fermented papermulberry tree of test example is prepared to prevention of sow constipation and reproductive performance
Test material：
The basically identical sow 80 of test and Selection kind, body weight, parity, is randomly divided into 4 groups：Former leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. group (uses structure
Fermented papermulberry tree powder in leaf powder alternative embodiment 6, other compositions are constant) (matched group), test I group (prepare by embodiment 5
Sow material), test II group (by embodiment 6 prepare sow material), test III group (by embodiment 7 prepare sow material).Often
Group 20, each repeats 1.Test and carry out in Guizhou Danzhai great Bei agriculture agricultural developments company limited, test period is 2016 3
10-May 10 moon.
Feeding and management
During test, each group rearing conditions are consistent；Each group gestation starts to feed nursing sow material on the 85th day, until under
Secondary breeding.Agent of feed for nursing sow scale of feeding was gradually lowered at antenatal 1 week, and childbirth same day feeding is reduced to 1.5kg, puerperal first three day
Gradually increase scale of feeding, free choice feeding after three days.Begin to use creep feed feeding piglet 1 week puerperal, it is until 21 ages in days wean, young
Pig injects chalybeate of enriching blood, 7 ages in days inoculation swine Fever Vaccine, the pseudo- mad vaccine of 15 age in days collunariums after being born 3 days.
Testing index
During test, strict record items test data, essential record tests below index：Sows in lactation day searches for food
Amount, prevention of sow constipation situation, sow lacation, go out cub's number, go out the young number of life, piglet birth counterpoise, piglet diarrhea rate, wean
Son's number living and ablactational baby pig average weight.
Record each and repeat the daily total constipation head number of sow, calculate constipation frequency.Wherein constipation frequency=often repeats sow
There is head day sum/(sow head number × test natural law) × 100% of constipation.
Wherein diarrhea frequency=often repeat piglet occur diarrhoea head day sum/(piglet head number × test natural law) ×
100%.
Data analysiss
Significance analysis are carried out using the independent sample T testing procedures in 17.0 statistical softwares of SPSS, with P<0.05 conduct
Significance of difference criterion.As a result represented with mean+SD.
Result of the test
Knowable to following table data：Using after the agent of feed for nursing sow that the present invention makes, sow is from pregnant 85th day to childbirth
21 days feces are significantly improved afterwards, and the average daily feed intake of sow in lactation also increases, wherein test II group significantly improving
20.11% (P≤0.01).Additionally, go out after addition fermented papermulberry tree the young number of life, the individual counterpoise of birth, 21 ages in days live young number,
21 ages in days individuality counterpoise and survival rate are above matched group, and piglet diarrhea rate is below matched group.Wherein, 21 days of II group are tested
Age individuality counterpoise improves 7.90% (P≤0.01).For synthesis, the effect for testing II group (10% fermented papermulberry tree) is managed the most
Think.
The impact of nursing sow batch prepared by 2 fermented papermulberry tree powder of table to prevention of sow constipation and reproductive performance
Conclusion
By contrast test, the nursing sow material that fermented papermulberry tree is prepared can substantially reduce sending out for prevention of sow constipation frequency
Raw 16.6～29.43% (P≤0.01), can substantially increase the feed intake 15.61～20.11% (P≤0.01) of sows in lactation,
Which gives birth to the young number of piglet work and nascent individuality counterpoise is above matched group, and improves survival rate, and the individuality of 21 age in days piglets
Counterpoise 1.44～7.90%.Meanwhile, more preferably, Incidence of Diarrhea is reduced piglet regularity.Illustrate after fermentation, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. nutrition
Value is improved, and the digestibility such as protein, crude fibre increases, while palatability is greatly improved, fermented papermulberry tree can be preferably
Originate as the albumen feedstuff of sow, have preferable effect to improving sow production performance.Wherein, the fermentation Broussonetia papyrifera of addition 10%
Leaf best results.
The breastfeeding sow compound feed effect evaluation and test of 3 present invention of test example
EXPERIMENTAL DESIGN
The basically identical sow 40 of test and Selection kind, body weight, parity, is randomly divided into 2 groups：Customary commercial nursing sow
Batch group (matched group), breastfeeding sow compound feed of the present invention (test group).20 per group, each repeats 1.Test in expensive
State Danzhai great Bei agriculture agricultural developments company limited carries out, and test period is 20 days-July 20 May in 2016.
3 customary commercial nursing sow batch formula table of table and trophic level
| Project | Content % |
| Semen Maydiss (two grades) | 58.80 |
| Bean cake (43%) | 23.00 |
| Wheat bran | 7.00 |
| Testa oryzae | 6.00 |
| Fish flour (CP >=65%) | 1.50 |
| Soybean oil | 0.16 |
| 98.5% lysine hydrochloride | 0.23 |
| Stone powder | 0.83 |
| Sodium bicarbonate | 0.12 |
| Calcium hydrogen phosphate | 0.90 |
| Sal | 0.35 |
| Premix material | 1.00 |
| Trophic level | |
| NE(kcal/kg) | 2387 |
| Crude protein | 17.88 |
| Lysine | 1.10 |
| Threonine | 0.68 |
| Methionine | 0.33 |
| Calcium | 0.76 |
| Total phosphorus | 0.66 |
Note：120g containing Fe in every kilogram of premix material, Zn 150mg, Mn 40g, Cu 45g, I 60mg, VA 816250IU,
VD323188IU, VE 3078IU, VK 560mg, VB1640mg, VB21.6g, VB3460mg, VB4280mg, VB5
600mg,VB117.7mg, VH 6mg, V1266.8mg, choline chloride 165g, balance of carrier.
Feeding and management
During test, each group rearing conditions are consistent；Each group gestation starts to feed nursing sow material on the 85th day, until under
Secondary breeding.Agent of feed for nursing sow scale of feeding was gradually lowered at antenatal 1 week, and childbirth same day feeding is reduced to 1.5kg, puerperal first three day
Gradually increase scale of feeding, free choice feeding after three days.Begin to use creep feed feeding piglet 1 week puerperal, it is until 21 ages in days wean, young
Pig injects chalybeate of enriching blood, 7 ages in days inoculation swine Fever Vaccine, the pseudo- mad vaccine of 15 age in days collunariums after being born 3 days.
Testing index
It is during test, strict to record every test data, essential record or the following index of measure：Micro- life in sow feces
Thing flora, sows in lactation daily ingestion amount, prevention of sow constipation situation, sow lacation, go out cub's number, go out the young number of life, piglet
Birth counterpoise, piglet diarrhea rate, wean son's number living and ablactational baby pig average weight.
Record each and repeat the daily total constipation head number of sow, calculate constipation frequency.Wherein constipation frequency=often repeats sow
There is head day sum/(sow head number × test natural law) × 100% of constipation.
Wherein diarrhea frequency=often repeat piglet occur diarrhoea head day sum/(piglet head number × test natural law) ×
100%.
Microorganism species in sow feces：In sow suckling the 14th day, the fresh excrement of two groups of each random collectings, 6 sows
Just, the measure with the method for plate culture count to fecal microorganism flora.Escherichia coli adopt maconkey agar culture medium culturing, bud
Spore bacillus is adopted MRS culture medium culturings, is adopted using bacillus culture medium (being purchased from the rich biology in Qingdao sea) culture, lactobacilluss
Ten times of gradient dilution counting methods carry out flora counting, at 37 DEG C, escherichia coli and lactobacilluss Anaerobic culturel 48h, and bud
Spore bacillus 24h, records clump count, common logarithm value lg (CFU/g) table of fecal microorganism Microflora colony-forming units
Show.
Data analysiss
Significance analysis are carried out using the independent sample T testing procedures in 17.0 statistical softwares of SPSS, with P<0.05 conduct
Significance of difference criterion.As a result represented with mean+SD.
Result of the test
Knowable to 4 data of table：After the agent of feed for nursing sow that the feeding embodiment of the present invention makes, indices have different journeys
The improvement of degree.Wherein, prevention of sow constipation frequency declines 26.75% (P≤0.01), and the average daily feed intake of sow in lactation is significantly improved
20.71% (P≤0.01).The individual counterpoise of birth and the individual counterpoise of 21 ages in days wean be respectively increased 8.33% (P≤0.05),
9.62% (P≤0.01).Additionally, going out the young number of life, the young number of 21 ages in days work and survival rate is above matched group, piglet diarrhea rate is low
In matched group.
The comparison of 4 nursing sow material of the present invention of table and customary commercial nursing sow material production performance
| Project | Matched group (n=20) | Test group (n=20) |
| Prevention of sow constipation frequency (%) | 4.15±2.51A | 3.04±1.35B |
| Go out the young number of life | 10.02±1.17 | 10.51±1.82 |
| The individual counterpoise (kg) of birth | 1.32±0.37a | 1.43±0.27b |
| The average daily feed intake of sows in lactation (kg) | 3.67±1.75A | 4.43±1.77B |
| The young number (head/nest) of 21 ages in days work | 9.62±1.10 | 10.32±1.52 |
| 21 ages in days individuality counterpoise (kg) | 5.54±1.15a | 6.13±1.66b |
| 21 age in days survival rates (%) | 96.01 | 98.19 |
| Piglet diarrhea frequency (%) | 4.16 | 3.29 |
The comparison of microorganism species in 5 two groups of nursing sow feces of table
| Project | Matched group (n=20) | Test group (n=20) |
| Escherichia coli (CFU/g) | 8.60±0.065a | 8.31±0.038b |
| Lactobacilluss (CFU/g) | 6.98±0.024a | 7.34±0.046b |
| Bacillus cereuss (CFU/g) | 7.12±0.014A | 7.76±0.062B |
As shown in Table 5, matched group is compared, escherichia coli bacterium in the sow feces of breastfeeding sow compound feed of the present invention is fed
Group's quantity reduces 3.37% (P≤0.05), lactobacilluss and bacillus cereuss Microflora be respectively increased 5.16% (P≤
0.05), 8.99% (P≤0.01).As a result show, the sow feed of the present invention with the addition of new microbial ecological agent, it is female improving
Pig fecal bacteria, in terms of maintaining intestinal health, with significantly effect.
The breastfeeding sow compound feed choice materials of the present invention, design science, comprehensively.Feedstuff is come from various crude fibres
Source, abandons Testa oryzae and wheat bran that perishable, mycotoxin is difficult to control, various crude fibre source structure, to preventing prevention of sow constipation
Effect is more projected, meanwhile, add the palatability that Sugar cane molasses improve feedstuff, effectively improve the feed intake of sow；Add expanded
Semen sojae atricolor and Semen Lini, central unsaturated fatty acid can promote breast cell proliferation, fetal development；Microbial ecological agent is added, is adjusted
Whole microbial bacteria group structure, maintains intestinal health, improves digestibility of the sow to feedstuff.Result of the test shows, with customary commercial
Nursing sow material is compared, and the agent of feed for nursing sow of the present invention can reduce prevention of sow constipation frequency 26.75% (P≤0.05), age of sucking
The average daily feed intake of sow significantly improves 20.71% (P≤0.01).The individual counterpoise of birth and the individual counterpoise difference of 21 ages in days wean
Improve 8.33% (P≤0.05), 9.62% (P≤0.01).Additionally, it is equal to go out the young number of life, the young number of 21 ages in days work and survival rate
Higher than matched group, piglet diarrhea rate is less than matched group.In terms of sow intestinal microflora is improved, the nursing sow of the present invention
Material can substantially reduce fecal coli quantity 3.37%, improve lactobacilluss and bacillus cereuss quantity 5.16% (P≤0.05),
8.99% (P≤0.01).Therefore, nursing sow material of the invention can preferably prevent prevention of sow constipation, improve enteric microorganism bacterium
Group, maintains intestinal health, improves sow feed intake and milk water quality, increases piglet birth weight, wean individual weight and survival rate, enters
And lift pig farm productivity effect.
Obviously, the above embodiment of the present invention is only intended to clearly illustrate example of the present invention, and is not right
The restriction of embodiments of the present invention, for those of ordinary skill in the field, may be used also on the basis of the above description
To make other changes in different forms, all of embodiment cannot be exhaustive here, it is every to belong to this
Bright technical scheme it is extended obvious change or change still in protection scope of the present invention row.
Claims (10)
1. a kind of agent of feed for nursing sow, it is characterised in that the raw material containing following weight portion：Fermented papermulberry tree powder 5-15 parts, jade
Rice 20-50 parts, brown rice 5-10 parts, puffing Semen sojae atricolor 4-6 parts, beet pulp 3-4 parts, Semen Lini 2-3 parts, soybean cover 3-5 part, Alfalfa
Powder 3-4 parts, cane molasses 2-3 parts, calcium hydrogen phosphate 0.8-1.2 parts, stone powder 0.8-1.2 parts, high temperature resistant phytase 0.01-0.03
Part, microbial ecological agent 0.01-0.04 parts, Sal 0.3-0.5 parts, lysine 0.2-0.6 parts, threonine 0.06-0.1 parts, egg ammonia
Sour 0.05-0.15 parts, choline chloride 0.1-0.2 parts, antifungus agent 0.05-0.25 parts, compound premixed feed 0.2-0.6 parts and anti-
Oxidant 0.02-0.07 parts.
2. agent of feed for nursing sow according to claim 1, it is characterised in that the raw material containing following weight portion：Fermentation structure
10 parts of leaf powder, 40.3 parts of Semen Maydiss, 6.5 parts of brown rice, 18 parts of bean cake, 5.5 parts of puffing Semen sojae atricolor, 3.5 parts of beet pulp, Semen Lini 3.22
Part, 3.8 parts of soybean cover, 4 parts of alfalfa meal, 2 parts of cane molasses, 0.86 part of calcium hydrogen phosphate, 0.84 part of stone powder, high temperature resistant phytase
0.01 part, 0.41 part of Sal, 0.03 part of microbial ecological agent, 0.33 part of lysine, 0.1 part of threonine, 0.04 part of methionine, chlorination
0.15 part of choline, 0.1 part of antifungus agent, 0.25 part of compound premixed feed, 0.06 part of antioxidant.
3. agent of feed for nursing sow according to claim 1 and 2, it is characterised in that described fermented papermulberry tree powder is by as follows
It is prepared by method：After the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of dries pulverizing is mixed with the glucose solution of 5-10%, aspergillus niger and bacillus subtilises are added
Mixed bacteria liquid, after stirring, control material water content is 40～50%, and constant temperature koji tray solid fermentation is carried out after fermentation ends
Drying and crushing.
4. agent of feed for nursing sow according to claim 3, it is characterised in that the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is mixed with glucose solution
Composition and division in a proportion example is mass ratio 2:1.
5. agent of feed for nursing sow according to claim 3, it is characterised in that by aspergillus niger bacterium solution and bacillus subtilises bacterium
Liquid is 2 in mass ratio:1 ratio is mixed into mixed bacteria liquid, and mixed bacteria liquid inoculum concentration is 10%～15%, wherein, the black fermented preparation
The viable count of mycete liquid is 1 × 106～7CFU/mL, the viable count of the bacillus subtilises bacterium solution is, 1 × 107～8CFU/mL。
6. agent of feed for nursing sow according to claim 3, it is characterised in that fermentation temperature is 30～37 DEG C, fermentation time
For 4～8 days.
7. agent of feed for nursing sow according to claim 1 and 2, it is characterised in that the compound micro-ecological preparation is by prebiotic
Bacterium and adjuvant are made, and the probiotic bacteria is by Lactobacillus plantarum (lactobacillus plantarum) CGMCC No.11262, withered
Careless bacillus cereuss (Bacillus subtilis) CGMCC No.11261 and Bacillus pumilus (Bacillus pumilus)
CGMCC No.12197 are constituted, in every gram of compound micro-ecological preparation, Lactobacillus plantarum (lactobacillus plantarum)
The viable count of CGMCC No.11262 is 75-100 hundred million, bacillus subtilises (Bacillus subtilis) CGMCC
The viable count of No.11261 be 25-75 hundred million, the viable bacteria of Bacillus pumilus (Bacillus pumilus) CGMCC No.12197
Number is 75-125 hundred million.
8. agent of feed for nursing sow according to claim 1 or claim 2, it is characterised in that in every kilogram of compound premixed feed
Contain：Vitamin A 0.08-0.28g, vitamin D30.024-0.1424g, Vitamin E 1.3-7mg, vitamin K3 3.36-
4.8g, vitamin B12-3.5g, vitamin B212-14g, vitamin B67-9g, vitamin B1238-46mg, nicotiamide 56-
67g, D-VB5 calcium 30-42g, Folic Acid 10.5-12.4g, biotin 1.05-2.5g, copper sulfate 3-12.5g, ferrous sulfate 30-
165g, zinc sulfate 50-100g, manganese sulfate monohydrate 25-85g, sodium selenite 65-320mg, calcium iodate 0.5-5g, balance of carrier
Powdered rice hulls.
9. the preparation method of the agent of feed for nursing sow described in any one of claim 1-8, it is characterised in that by Semen Maydiss, bean cake Jing
Cross after primary dcreening operation processes, powdering is crushed with the sieve of 3.0mm, enter and treat batch bin, it is by the Semen Maydiss after crushing, brown rice, bean cake, sweet
Rapeseed cake, Semen Lini, soybean cover and fermented papermulberry tree powder, puffing Semen sojae atricolor and alfalfa meal are proportionally added into mixing by Fructus Anisi Stellati burden balance
Conjunction machine, then respectively by calcium hydrogen phosphate, stone powder, Sal, lysine, methionine, threonine, compound premixed feed, chlorination gallbladder
Alkali, phytase, compound micro-ecological preparation, antifungus agent, antioxidant are weighed from micro-balance or manually and add mixer, mixing 10-
Pelletized into storehouse to be pelletized after cane molasses mix homogeneously is added after 15 seconds.
10. the preparation method of agent of feed for nursing sow according to claim 9, it is characterised in that grinding particle size 3.0-
4.0mm, through the incorporation time mix homogeneously of 90-150 seconds, under conditions of vapour pressure 0.2-0.25Mpa, temperature 60-85 DEG C
Pelletized, particle diameter is 3.5-4.0mm, is >=5min cool time.
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