WO2013056455A1

WO2013056455A1 - Lipase feed additive, processing method and application thereof

Info

Publication number: WO2013056455A1
Application number: PCT/CN2011/081075
Authority: WO
Inventors: 时本利; 王剑英; 兰瑛; 陈立; 谢英明; 朱维叶
Original assignee: 深圳市绿微康生物工程有限公司
Priority date: 2011-10-20
Filing date: 2011-10-20
Publication date: 2013-04-25

Abstract

A feed additive containing lipase comprises the following components: 40 to 70 parts by weight of corn, 5 to 15 parts by weight of bean pulp, 1 to 5 parts by weight of fish flour, 15 to 23 parts by weight of wheat bran, 2 to 6 parts by weight of premix, and 0.01 to 0.05 part by weight of lipase, the lipase being made from modified penicillium expansum.

Description

Lipase feed additive, processing method and application thereof

Technical field

The invention relates to an animal feed containing lipase, in particular to an active ingredient derived from a feed for expanding Penicillium lipase, a processing method thereof and application thereof in animal breeding, and belongs to the technical field of bioengineering and feed processing. Background technique

Microbial lipases play an important role in various fields of production and life, and are currently used in industrial production for leather, food baking, detergents, oil processing and functional foods. It is also reported in the application of lipase to animal feeds. It is common to use lipase as a feed additive in combination with the main ingredient.

The principle of lipase as an animal feed additive is: Fat is an important energy supply and energy storage material for animal organisms. It can provide essential fatty acids to the body, has the nutritional and physiological functions of regulating body metabolism, and is an essential nutrient for animals. Lipase is the most basic enzyme used in fat digestion. It can hydrolyze fat into free fatty acids, glycerol and monoglycerides for animal absorption and utilization. Monogastric animals can secrete lipase by themselves, but the digestive function of young animals is not well developed, endogenous digestive enzymes are insufficiently secreted, and fat digestion and absorption rate is low. The value of adding lipase to the diet is to address the shortcomings of the animal's endogenous digestive enzyme deficiency, thereby improving the digestion and absorption of fat, reducing the incidence of intestinal diseases in animals, improving the energy utilization of fat in feed, and promoting fat. Absorption of soluble vitamins, exerting animal production potential and reducing feed costs.

Chinese patent application (publication number CN1 01204198A) discloses a feed additive using 0.1% lipase for 28-day-old weaned piglets. The main ingredients of the feed include corn and suckling pig concentrates, and the feed also includes fat powder. The results of the feeding showed that the feed promoted the digestion and absorption rate of the piglets and improved the performance of the piglets. The Chinese patent application (publication number CN1 01961 068A) discloses a compound enzyme feed additive for pigs, including lipase, cellulase, mannanase and xylanase, and also provides the utilization efficiency of pig feed digestion and Improve the body's immune function. However, the lipase-containing feed provided in the above patent application has a disadvantage that the cost of the lipase source is high, the industrialization is difficult, and the digestion and absorption rate of the animal is not high, which is not conducive to large-scale popularization and application. In addition, in recent years, with the rising price of energy feed materials, especially oils and fats, how to improve the utilization of energy in feed, reduce the amount of oil added, avoid the harm caused by high oil, save costs, and become the focus of attention. .

Summary of the invention

The present invention is directed to the above-mentioned deficiencies existing in the field of feed additives, and provides an active ingredient from a feed additive for expanding Penicillium lipase, a method for producing the same, and an application thereof as a plurality of animal feed additives. .

One of the objects of the present application is to provide a lipase feed additive, wherein the feed additive comprises the following components in parts by weight:

40 to 70 parts of corn and 5 to 15 parts of soybean meal,

1 to 5 servings of fishmeal and 15 to 23 parts of wheat bran,

Premix 2 ~ 6 parts, lipase 0. 01 ~ 0. 05 parts.

Further, the lipase is a lipase produced from a modified Penic i I ium expansum.

Among them, the modified nucleotide sequence of the Penicillium lipase gene, the construction and expression method, and the amino acid sequence of the modified modified Penicillium lipase have been applied in the applicant's previous application "modified Penicillium lipase The gene and its construction and expression method are fully disclosed in the application date of July 1, 2011, application No. 201110183472. 1 , and the modified extended Penicillium lipase can be obtained by a person of ordinary skill in the art according to the above application.

Another object of the present invention is to provide a method for processing a lipase in a feed additive comprising a modified Penicillium sp. (Pen i c i 11 i expus) lipase, which comprises the following steps:

1. Preparation before production:

Check the valves of the tank for leaks, ensure no leakage, ensure the air holding pressure test of the tank, ensure that the tank has no leakage and the mechanical seal is intact, and keep the air system disinfected at 121 °C for 30 minutes; 2, the real tank disinfection:

Indirect heating from the interlayer to the temperature of 90 ° C, and then steam from the sampling tube, the inlet pipe, the transfer pipe, direct heating, while closing the sandwich steam into the valve, heating to 121 ° C, for 30 min, in At the end of sterilization, the cooling system is immediately turned on for cooling. Before introducing sterile air, the pressure in the tank must be lower than the filter pressure of the sterile air, the temperature is lowered to 28 °C, and the flame is inoculated;

3. Seed culture:

The process requires a culture temperature of 28 °C, a tank pressure of 0. IMpa, aeration volume of 18 cubic meters per hour, and a culture time of 24 hours, to be transplanted;

4, transplanting pipeline disinfection:

The steam is introduced from the relevant pipe, so that the steam pressure in the pipe reaches 0. 147MPa, which is maintained for 45min. The sterilization process discharges air from the relevant valve and the side valve, and the steam passes through the dead angle sterilization. After the sterilization is completed, the steam is turned off, and the pipe is to be in the pipe. When the pressure is lower than the pressure of the air filter, it is filled with sterile air to maintain a pressure of 0. 098MPa, and the pressure is reserved for use;

5, transplant:

According to the process requirements, when the transplanting pipeline and the medium reach the required temperature, the seeding is started, the pressure of the upper tank is increased, and there is a certain pressure difference. When the liquid is moved, the first tank can be closed and the valve is closed. Level shifting valve, after the seeding is finished, the steam is passed into the transplanting pipeline for disinfection;

6. Fermentation culture:

The process requires a culture temperature of 28 °C, a tank pressure of 0. IMpa, aeration volume of 120 cubic meters per hour, and a culture time of 50 hours, which is to be filtered by the plate;

7, plate frame filtering:

After the fermentation is finished, the fermentation tank discharge valve is opened, the fermentation hydraulic pressure is used to push the fermentation hydraulic pressure into the plate frame filter machine, the plate frame filter press discharge valve is opened, and the liquid lipase is pressed into the storage tank to be concentrated;

8. Ultrafiltration concentration:

According to the enzyme activity and product requirements, determine the concentration multiple, concentrate according to the ultrafiltration machine operating procedures, thick After the shrinking is completed, the concentrate is pumped into the storage tank to be mixed; the feed pressure is 2 Mpa, and the discharge pressure is 1. 5 Mpa;

In the mixer cylinder container, after adding a certain amount of dry starch, screwing on the gland, sealing it, opening the drive motor, raising the concentration of the concentrate tank to 0.1 Mpa, turning on the atomizer, you can start Operation, mixing for 20 minutes, after the materials have been mixed to meet the requirements, the drive motor can be turned off, the control power is turned off, the butterfly valve is opened, and the material is poured out.

Further, the seed tank production ingredients include the following components:

Bean cake powder 3. Okg, yeast powder 0. lkg,

Tap water 180L, starch 1. 0kg.

Further, the fermenter ingredients include the following components:

Bean cake powder 50. 0kg , yeast powder 1. 0kg,

Tap water 1600 L, starch 10. 0kg.

Another object of the present application is to provide the use of the feed additive comprising the modified Penicillium expansum lipase in animal feeding.

Specifically include:

1. The application of piglets in the pre-conservation period can reduce the feed-to-meat ratio, and reduce the feed cost of piglets' weight gain while saving the cost per ton of feed;

2. The effects on the reproductive performance of lactating sows and the performance of piglets, including increasing the weaning weight of piglets, increasing the weight gain of piglets and significantly shortening the interval of returning sows;

3. Significantly increase the lactation volume of the sows and the birth weight of the piglets, significantly reduce the mortality rate of the piglets during the lactation period, shorten the postpartum estrus interval of the sows, shorten the weaning age and increase the weaning weight.

detailed description

Hereinafter, the lipase feed additive, the processing method and the application thereof described in the application of the present invention are described in conjunction with specific embodiments, in order to better understand the technique of the present application. The solution, rather than the limitation of the technical content, in fact, on the same or similar principle, the modification of the feed additive component, the processing and manufacturing process conditions and the process are improved to achieve substantially the same effect. The purpose is all within the technical solution claimed in the application of the present application.

Embodiment 1

A lipase feed additive, wherein the feed additive comprises the following components in various weight percentages:

40 servings of corn and 5 servings of soybean meal,

1 serving of fishmeal and 15 servings of wheat bran,

Premix 2 parts, lipase 0.1 parts.

Embodiment 2

70 servings of corn and 15 servings of soybean meal,

5 parts of fishmeal, 23 parts of wheat bran,

Premix 6 parts, lipase 0. 05 parts.

Embodiment 3

A lipase tanning additive, wherein the feed additive comprises the following components in various weight percentages:

50 servings of corn and 10 servings of soybean meal,

3 parts of fishmeal, 18 parts of wheat bran,

4份。 Premix 4 parts, lipase 0. 03 parts. Embodiment 4

The method for processing a fatty enzyme preparation in a feed additive comprising a modified Penicillium expansens (Penicilli strain expans) lipase comprises the following steps:

1. Preparation before production:

Check the valves of the tank for leaks, ensure no leakage, ensure the air holding pressure test of the tank, ensure that the tank has no leakage and the mechanical seal is intact, and keep the air system disinfected at 121 °C for 30 minutes;

2. Real tank disinfection:

Indirect heating from the interlayer to the temperature of 90 ° C, and then steam from the sampling tube, the inlet pipe, the transfer pipe, direct heating, while closing the sandwich steam into the valve, the temperature is raised to 121 ° C, for 30 min, At the end of sterilization, the cooling system is immediately turned on for cooling. Before introducing sterile air, the pressure in the tank must be lower than the filter pressure of the sterile air, the temperature is lowered to 28 ° C, and the flame is inoculated;

3. Seed culture:

Process requires culture temperature 28 ° C, tank pressure 0. IMpa, ventilation 18 cubic / hour, incubation time 24 hours, to be transplanted;

4. Displacement pipeline disinfection:

Steam is introduced from the relevant pipeline so that the steam pressure in the pipe reaches 0.147 MPa for 45 minutes. The sterilization process removes air from the relevant valves and side valves, and the steam passes through the dead angle sterilization. After the sterilization is completed, the steam is closed. Below the air filter pressure, the sterile air is pressurized to 0.098 MPa, and the pressure is maintained for use;

5. Transfer:

6. Fermentation culture:

The process requires a culture temperature of 28 ° C, a tank pressure of 0. IMpa, aeration of 120 cubic meters / hour, incubation time 50 hours, to be filtered by the frame;

7. Board frame filtering:

8. Ultrafiltration concentration:

According to the enzyme activity and product requirements, determine the concentration multiple, concentrate according to the ultrafiltration machine operating procedure, concentrate the concentrate into the storage tank to be mixed; the feed pressure is 2 Mpa, the discharge pressure is 1. 5 Mpa;

9. Mix:

Embodiment 5

Application of feed lipase in piglets (5-7 weeks old) in the pre-conservation stage:

I. Materials and methods

1. Test materials: Nursery piglets, lipase (provided by Shenzhen Green Bioengineering Co., Ltd.).

2. Test time: A total of 17 days, of which the pre-feeding period is 3 days.

3. Test site: Yangpeng Breeding Farm of Liangzhu Original Breeding Farm of Guangxi Nongken Yongxin Animal Husbandry Group.

4. Test and diet preparation

Twenty-eight-day-old pigs with close weights were randomly divided into two treatment groups, the control group and the experimental group. Each treatment group had 3 replicates (column), and 30 piglets per replicate. Each treatment group was fed a different diet, in which the control diet was the basal diet, and the experimental group was able to reduce the 50 kca l/kg energy value + 200 mg/kg lipase by adjusting the fat addition amount on the basal diet. The nutrient value of the test diet formula is shown in Table 1. The premixes required for the test are produced by Green Company and re-tested according to the A and B numbers. The full price of the corresponding test is produced by the Liangzhu Original Breeding Farm Feed Factory. Table 1 Dietary formula and nutritional value of the nursery stage test

^if « Pre-care formula

Yuan/kg control group

Corn 2. 36 540 550

Cardamom 3. 2 90 90

Puffed soybeans 5 1 00 100

Milk replacer 7. 8 120 120

Secondary powder 2. 3 30 30

Fishmeal 1 3. 9 50 50

Soybean oil 1 0. 5 20 10

GL502A 14. 5 50

GL502B 50

Total 1000 1 000

Formula cost (yuan / ton) 4697. 5 4640

Digestive energy (kca l /kg ) 3275 3221

Crude protein (%) 21. 0 21. 0

Lysine (%) 1. 51 1. 52

Ileum digestible lysine (%) 1. 06 1. 06 ileal digestible methionine (%) 0. 30 0. 30 ileal digestible threonine (%) 0. 80 0. 80 ileal digestible tryptophan ( %) 0. 18 0. 18

5, feeding management

The test pigs were carried out in the same nursery, according to the current feeding mode of the farm; the piglets were grouped and pre-fed separately for the test materials before the start of the test, and the piglets were weighed on an empty stomach the next morning after the pre-feeding period. The trial was officially started; during the test period, the pigs were allowed to eat and drink freely. The feed intake and diarrhea of each piglet were recorded every day. The piglets were weighed on the morning of the next morning after the test. If the pig died during the trial, the date of death and weight were recorded.

6, measurement indicators and calculation formula

Head average daily feed intake, head daily gain, feed to weight ratio, weight gain cost, diarrhea rate and skin tone. Among them, the diarrhea rate is calculated according to the following formula.

(number of diarrhea * days of diarrhea) *100

Diarrhea rate (%) = ∑

Total number of trials * number of trial days

7, statistical analysis of data

3⁄4 ^ data was first sorted and preliminary calculated with Excel2003, and then analyzed by DPS statistical analysis software for variance analysis. The Duncan method was used for multiple comparisons, and the data was expressed by the average standard error (X Shi SE).

Second, the results and analysis

1, 3⁄4 ^ fruit

The effect of feed lipase on the growth performance of pre-conservation piglets is detailed 2 .

Table 2 Effect of feed lipase on growth performance of pre-conservation piglets

Group/indicator Control group Experimental group Number of heads 105 103

Days 14 14 Test initial weight (kg) i ₃ .31 ±0.05 ^a 13.01 ±0.36 ^a test end weight (kg) ₂₀ . _{37 ± 0} . ₅₅ ^a 19.98±0.5i ^a average daily gain (kg/d) o. _{505 ± 0} . ₀₄ ^a 0.4 _{98 ±} 0. n ^a Average daily feed intake (kg/d)

0.729 + 0.01 0.651 + 0.01

Material to weight ratio

1.461士 0.04 1.311士 0.11 Weight gain cost (yuan/kg)

6.863 ± 0.52 6.073 ± 0.18 diarrhea rate (%)

1.05 ± 0.59 0.82 ± 0.37 Note: The same letter is the same, the difference is not significant (P > 0.05), the same below.

2, the result analysis

2.1 Average daily feed intake

Compared with the control group, the test group decreased by 78 g / day, a decrease of 10.7%, the difference was extremely significant (P < 0.01) ₀ 2.2 head daily gain

Compared with the control group, the test group decreased by 7 g / day, 1.4%, and the difference was not significant (0.05).

2.3 material to weight ratio

Compared with the control group, the test group decreased by 0.150: 1, and the score decreased by 10.3%.

2.4 unit weight gain cost

Compared with the control group, the test group decreased by 0.78 yuan / kg, a decrease of 11.4%.

2.5 diarrhea rate

Compared with the control group, the experimental group of diarrhea of piglets decreased 21.9%, but no significant difference between (0.05) ₀

2.6 skin tone

In terms of piglet skin color, the test group was better than the control group when observed by the test site technician. Third, the technical wire

The results of this experiment showed that the digestion energy of 50kcal/kg was reduced on the basis of the diet of the control group. With 200g/p 屯 lipase, the daily average feed intake index of piglets decreased compared with the control group. The average weight gain of piglets was slightly lower than that of the control group, but the difference was not significant. The lipase group reduced the feed-to-meat ratio and the unit weight gain feed cost. Although the difference was not significant, the economic benefits were considerable. The diarrhea rate of the test group was lower than that of the control group, but the difference was not significant. The piglets in the experimental group also had better skin color than the control group.

In summary, the application of lipase in nursery pigs was successful. The lipase group was better than the control group, which could reduce the feedstock ratio by 0.1 or more, and reduced the feed cost of piglet weight gain while saving. The cost per p屯 feed is nearly 50 yuan.

Embodiment 6

Effects on reproductive performance of lactating sows and performance of piglets

1 Materials and methods

1. 1 Test material: Lienkang (provided by the Shenzhen Green Micro-Korean Bioengineering Co., Ltd.) containing the modified Penicillium sp.

1. 2 Test site: Fuqing City Fengze Agriculture and Animal Husbandry Technology Development Co., Ltd. The first pig farm 1. 3 Test:

Choose healthy, poor physical condition, 30 pairs of 3-8 sows, and randomly group according to public opinion. There are three treatment groups, I, II, and III, each with five replicates and two replicates each. The three feeding materials A, B and C were fed separately, and the whole test was blindly tested. 1. 4 test diet

Table 3. Test grouping

Group test material

Group A, control group

Group B +200g / t Li Nengkang

Group C +300g / t Li Nengkang

1. 5 Measurement indicators Sows, feed intake, piglet birth weight, piglet weaning weight, weaning weight gain.

1. 6 Data statistics The data were analyzed by sps s l 3. 0, and one-way ANOVA was used for significance test (LSD method). 2 test results

Table 4. Summary of the indicators for the whole process of the test

Item A (Control) B ( 200g Li Nengkang) C ( 300g Li Nengkang) The average weight of the primary is 1. 5312 ± 0. 17530 1. 4804 ± 0. 26631 1. 5215 ± 0. 22651 Band number 10. 6000 + 2. 06559 10. 8000 士士

0. 87560

Actual initial weight 1. 5300士0. 17372 1. 4560士0. 26442 1. 5540士0. 20506 After a week, the weight is 2.6004 ± 0. 12354 2. 2519 ± 0. 44771 2. 9583 + 0. 34340 After two weeks Weight 4. 3236 ± 0. 26894 4. 2466 ± 0. 63878 4. 6635 ± 0. 44558

14-21日增重1. 782士0.: 2020 2. 232 + 0. 3075 2. 312 + 0. 5229 Weaning weight 6.1062 ± 0. 51838 6. 4790 ± 6. 9757 ± 0. 99637 Weaning Weight gain 4. 5768 ± 0. 44188 5. 0234 ± 0. 71305 5. 4212 ± 0. 48970 Average feed intake 5. 9440 ± 0. 27302 5. 4900 ± 5. 9130 ± 0. 61646

0. 73087

Reciting (16,000 before the birth of 16.6000 ± 16. 4000 months) 2. 45855 3. 59011 3. 89301

Reciting (1,1000, 16.16, 6,000 days before birth) 3. 10734 4. 22164 4. 45845

Reciting (weaning) 14. 6000 士 14. 9000 士

3. 16930 2. 49666

Weaning mating interval 5. 0556 士 0. 63465 4. 6111 士 0. 48591 4. 5556 士 0· 30046

(days)

3 Results analysis and economic benefit analysis 3.1 Results analysis Through this test, the following conclusions can be drawn:

1. Adding different concentrations of Lienkang in the lactating sow feed can increase the weaning weight of piglets, the difference is significant (P < 0.05), of which 2002.8g is increased by 372.8g in the 200g/t group; 25.95g is increased in the 300g/t group. %.

2. In the stage where the sow milk is most depleted in the 14-21 days after the birth of the piglet, in this stage, the addition of different concentrations of lienkang in the lactating sow feed can increase the weight gain of the piglets, and the difference is extremely significant (P< 0.01). ), wherein 200g/t group increased 450g accounted for 6.11%; 300g/t group increased 530g accounted for 14.24%.

3. The addition of different concentrations of Lienkang in lactating sows had no significant effect on sow feed intake during the whole experimental period (P>0.05), and the 300g/t group had a tendency to increase feed intake.

4, the addition of different concentrations of Lienkang in the lactating sow feed can significantly shorten the sow return interval (P <

0.05), the 200g/t group shortened 0.4445 days to 8.79%, and the 300g/t shortened 0.5 days to 9.89%.

3.2 Analysis of economic benefits

Fujian Fengze Agriculture and Animal Husbandry Feed Technology Co., Ltd. has a scale of 3,000 sows per year. The annual use of Likenkang is 600 to 900kg, which is added at 200g/t. The added cost is 72,000 yuan. According to 300g/t ^力口, ^力口 is 108,000 L.

Weaned piglets are calculated at a market price of 30-30 kg/kg of 30 yuan/kg. The annual production of 3000 sows is calculated from 25 counts and 75,000 heads per year. Added by 200g/t, the minimum profit is 838,800 yuan; according to 300g/t, the minimum profit is 1,596,400 yuan.

This test shows that, if it is fully used in Fengze breeding farm, it can generate additional income: 200g/t added amount is 768,800 yuan; 300g/t added quantity is 1,488.4 thousand yuan, the effect is ideal, it is worth applying and popularizing. Example 7

Effect on the performance of breeding sows

1 Materials and methods 1. 1 test sample Likenkang (provided with the modified extended penicillium lipase described in the application of the present invention, provided by Shenzhen Luweikang Bioengineering Co., Ltd.)

1. 2 Test 3 Full time and place The breeding test lasted for 60 days and the test site was in a certain pig farm in Maoming. 1. 3 Trial 3 Whole Animals and Grouping 50 pregnant sows with similar maternal parity and healthy prenatal 30 days were randomly divided into 2 groups, 5 replicates in each group and 5 replicates in each group. The control group was fed a basal diet. The treatment group was the experimental group, and 200 g/t of Likenkang was added to the basal diet. The trial was conducted in two stages (30 days before delivery, 30 days after delivery) for a total of 60 days. 1. 4 Basic diets The basic diet formula and nutritional level in late pregnancy are shown in Table 5. Table 5 Basic diet composition (%) and nutrition level

Raw material ratio (%) nutrition level

Corn 61 Metabolizable Energy (Kca l /kg ) 3000

Cardamom 14 Crude protein (%) 16. 0 Fishmeal 2 Calcium (%) 0. 65 Wheat bran 19 Total phosphorus (%) 0. 54 Premix 4 Lysine (%) 0. 90

Proline (%) 0. 90

Total 100 Methionine (%) 0. 41

The formula and nutritional level of lactating sows are shown in Table 6 Table 6 Basic diet composition (%) and nutrition level

Raw material ratio (%) nutrition level

Corn 61 generation Xie Neng 3250

( Kc al/kg ) Cardamom 27 Crude protein (%) 17. 3 Grease 2 Calcium (%) 0. 65

Wheat bran 4 total phosphorus (%) 0. 54

Premix 6 Lysine (%) 1. 20 Proline (%) 1. 0

Total 100 Methionine (%) 0. 50

1. 5 Measurement indicators Record the number of births in the sow breeding stage; the birth weight of the piglets; the stillbirth rate of the piglets; the estrus interval of the sows; the diarrhea rate, mortality, weaning age and weaning weight of the piglets during lactation. 1. 6 Data Processing All data were analyzed by SPSS ( 10. 0 ) statistical processing software for variance analysis. LSD multiple comparisons were used. The test data was expressed as the mean standard error. 2 Results and analysis

2. 1 Effect of Lienkang on reproductive performance of sows Table 7.1 Effect of Lienkang on sow reproduction

Control group (basic day test group (basic diet + 200g / t benefit)

Food) can)

Average number of births (head) 10. 81 + 0. 53 11. 14 ± 0. 46

Piglet birth weight (kg) 1. 473 士 1. 581 + 0. 085a

0. 122

Stillbirth rate% 0. 059 士 0. 000 + 0. 000

0. 091

Weaning to estrus interval 5. 24 ± 0. 86 4. 19 ± 0. 45 a

(d) It can be seen from Table 7-1 that the addition of 200 g/t to the diet in the later stages of the sow is reflected in the test. The obvious advantage is that the average number of births is not significantly different from the control group. The number of fertilized eggs in the pre-pregnancy period directly affects the number of births. Therefore, the addition of Lienkang in the late pregnancy has little effect on the number of sows born. The difference between the experimental group and the control group is not significant. The newborns in the experimental group are significantly higher. In the control group, it showed that the piglets developed well in the sows. Li Nengkang helped the digestion and absorption of crude fat, so that the sows gained more energy and nutrients, which provided protection for the healthy development of the piglets. Practice has proved that piglets are born. The difference in weight directly affects the performance of pigs. The birth weight of piglets in the experimental group is significantly higher than that of the control group, which lays a solid foundation for the production of pigs. There is only one litter in the control group, and the difference between the test group and the test group is not significant. There were no stillbirths in the test group, indicating that Lienkang helped to maintain the growth and development of sows and piglets. The stillbirth in the control group may be caused by the stress of the sows in the middle and late pregnancy, and may also be related to the nutrition of the sows. The results from pig weaning to estrus interval showed that the test group was significantly lower than the control group, and the study showed that the sow was weaned to the estrus interval and the sow's body condition. Related, sow pregnancy and production need to consume a lot of energy and nutrients, postpartum lactation nutrition can not keep up with the body and body protein to maintain lactation needs, so the sow body condition often declines after delivery, the degree of fallout and pregnancy Later, it is related to the nutrition of lactation, which also directly affects the return of the sow. The experiment shows that the addition of Likenkang to the diet helps the sow maintain the body condition and lays a foundation for lactation and return.

2. 2 Effect of Likenkang on the performance of lactating sows Table 7. 2 Effect of Likenkang on the performance of lactating sows

S5

Indicator control group (basic day test group (basic diet + ² 00 g / 1 benefit)

Food) can)

;3⁄4|L 3⁄4 (sensory refers to +++ +++++ a

Standard)

Weaning weight of piglets (kg) 6. 253 士 7. 681 ± 0. 185a

0. 822 Weaning mortality rate 15. 8 ± 7. 1 2. 3 ± 1. 8 a

Weaning age (d) 23 23

Note: The difference between the superscript letters in the same column data is significant (P<0.05), and the difference is not significant (P>0.05).

It can be seen from Table 7-2 that the addition of Likenkang to the diet of the sows can significantly improve the performance of the sows. The milk production of the sows in the test group is significantly higher than that of the control group, because the sows cannot be breast-fed during lactation. Extrusion, the sow's milk production could not be directly measured. The piglets in the experimental group were less likely to change their breasts during breastfeeding than the control group, and the lactation time was longer than that of the control group. The piglets showed a satisfactory state after the lactation of the sows. In the control group, the frequency of piglets changing breasts was higher. The number and frequency of watering of piglets after sow lactation was higher, and the breasts of the sows were lower than those of the test group. The piglets in the experimental group and the control group were weaned on the 23rd. The weaning weight of the experimental group was significantly higher than that of the control group, and the mortality of weaning was significantly lower than that of the control group. Sows consume a lot of postpartum physical energy, and activities such as lactation require high nutrients from the diet. The energy needs of lactating sows are large, and the decrease in feed intake and dietary nutrient digestion and absorption directly affect the mother. The milk production in pigs, the higher mortality rate in the control group is mainly due to the higher rate of sows in the sows. The nutrition of the diet can not meet the needs of piglets. The malnutrition and diarrhea caused by dehydration during lactation are more likely. The intake of piglets caused by breastfeeding was insufficient. From the production, the weaning mortality of piglets increased, the weaning weight was lower than that of the test group, and the uniformity of piglets was not high. The addition of Likenkang helps the sow to digest and absorb the nutrients of the diet, thereby obtaining more nutrients. Weaning weight is also very important for improving the growth performance after weaning. Studies have shown that after weaning weight is increased from 3.8 kg to 6 kg, the weight gain during weaning to 42 days can be increased by 4.2 kg, or equivalent to the increase in weaning weight. At 1 kg, the daily gain is increased by 45 g/day. This has also been confirmed in other pig center research.

Many factors such as heredity, dietary quality, dietary composition, and the environment all affect the feed intake of lactating sows. The supply of feed energy has a great influence on the feed intake of sows at all stages, and the total duration of sows during lactation. Feed intake is positively correlated with lactation performance and subsequent reproductive performance, and sows need to maintain energy nutrition 5-10% higher during pregnancy than during pregnancy. Almost 75% of the energy needed for lactating sows is used for lactation. When the essential nutrients in the diet do not meet the production and even maintenance needs of the different stages of breeding sows, the sows will mobilize and consume the fat and protein of their tissues. To meet their nutritional needs during the breeding phase. Insufficient long-term nutrient intake will cause the sow to fall off. Excessive mites will not only affect the weight gain rate of the suckling piglets, but also seriously affect the production performance of the next pig's next child, so that the time from weaning to re-estrus is prolonged, and the conception rate is decreased. , litter size and piglet birth weight decreased. Therefore, meeting the nutritional needs of sows during lactation and ensuring better feed intake are the basis for maintaining good sow condition and optimal reproductive performance.

Energy is the most difficult dietary factor in lactating sow diets. In most cases, lactating sows are hard to eat enough feed to meet their daily energy needs, and lactating sows, due to maternal instinct, will consume the mother's own tissue nutrients to convert to meet basic lactation needs. When the energy intake increases, the weight loss during lactation decreases, which shortens the time of estrus after weaning. It can be seen that the feed intake during lactation has a profound effect on reproductive performance after weaning. The concentration of feed nutrients during lactation was negatively correlated with the amount of loss and weight loss during lactation, and had an effect on the interval between gestation periods after weaning. Increasing the energy concentration of the diet and the feed intake of the lactating sow can increase the energy and nutrient intake, which will effectively avoid the weight loss of the sow during lactation and ensure better reproductive performance of the lactating sow. In addition to adding high-energy raw materials such as oil and fat to the grain, increasing the digestibility of the diet can also increase the nutrient concentration of the diet. Adding Likenkang to the diet can significantly improve the diet. The digestibility of medium and thick fats, thereby increasing the energy concentration of the diet, confirms this conclusion. It has been reported that the feeding strategy during lactation is to maximize the food intake and maximize the energy demand, while the lack of nutrition during lactation will increase the weight loss of the sow, delay in estrus after weaning, and decrease the number of litters in the litter. At the same time, we must also fully consider the balance of other nutrients in the diet, in order to give full play to the breeding potential of lactating sows, shorten the sow estrus interval, increase the survival rate and litter weight of weaned piglets, thereby improving the reproductive performance of sows and The economic benefits of the pig industry. It can be seen that the addition of Likenkang to the feed can significantly increase the lactation volume of the sow and the birth weight of the piglets; significantly reduce the mortality rate of the piglets during the lactation period; shorten the postpartum estrus interval of the sows; shorten the weaning age and increase the weaning weight, for the meat The production of pigs lays a good foundation.

Claims

A lipase feed additive, characterized in that: the feed additive comprises the following components in parts by weight:

40 to 70 parts of corn and 5 to 15 parts of soybean meal.

1 to 5 servings of fishmeal and 15 to 23 parts of wheat bran,

Premix 2 ~ 6 parts, lipase 0.01 ~ 0.05 parts.

2. The lipase feed additive according to claim 1, wherein: the lipase is a lipase produced from a modified Penicillium sp.

3. The method for producing a lipase derived from a modified Penicillium sp. in a lipase feed additive according to claim 2, wherein the method comprises the steps of:

1) Preparation before production:

2) Real tank disinfection:

3) Seed culture:

4) Disinfection of transplanting lines:

Steam is introduced from the relevant pipeline so that the steam pressure in the pipe reaches 0.147 MPa for 45 minutes. The sterilization process removes air from the relevant valves and side valves, and the steam passes through the dead angle sterilization. After the sterilization is completed, the steam is closed. Below the air filter pressure, the sterile air is pressurized to 0.098 MPa, and the pressure is maintained for use; 5) Transfer:

6) Fermentation culture:

Process requires culture temperature 28 ° C, tank pressure 0. IMpa, ventilation 120 cubic / hour, incubation time 50 hours, to be framed;

7) Board frame filtering:

8) Ultrafiltration concentration:

According to the enzyme activity and product requirements, determine the concentration multiple, concentrate according to the ultrafiltration machine operating procedure, concentrate the concentrate into the storage tank to be mixed; the feed pressure is 2 Mpa, the discharge pressure is 1.5 Mpa;

9) Mixing:

In the mixer barrel, add a certain amount of dry starch, screw the gland, seal it, open the drive motor, raise the pressure of the concentrate tank to 0.1 Mpa, turn on the atomizer, and start operation. Mix for 20 minutes. After the material has been mixed to meet the requirements, the drive motor can be turned off, the control power is turned off, the butterfly valve is opened, and the material is poured out.

4. The method of manufacturing according to claim 3, wherein the production material in the seed tank comprises the following components:

Bean cake powder 2.0-5.0kg, yeast powder 0.05 ~ 0.20kg, tap water 150 - 200L, starch 0.8 ~ 1.5kg.

5. The method of manufacturing according to claim 3, wherein the ingredients in the fermentor comprise the following components:

Bean cake powder 40.0~ 80.0kg, yeast powder 0.5 ~ 2.0kg, Tap water 1400 ~ 1800 L, starch 8. 0 ~ 15kg.

6. The use of the lipase feed additive of claim 1 in animal feeding.

The application according to claim 6, wherein: the application specifically includes:

1) The application in the pre-conservation piglets can reduce the feed-to-meat ratio, and reduce the feed cost per pig's weight gain while saving the cost per feed.

2) The effects on the reproductive performance of lactating sows and the performance of piglets, including increasing the weaning weight of piglets, increasing the weight gain of piglets and significantly shortening the interval of returning sows;

3) Significantly increase the lactation volume of the sows and the birth weight of the piglets, significantly reduce the mortality rate of the piglets during the lactation period, shorten the postpartum estrus interval of the sows, shorten the weaning age and increase the weaning weight.