CN101053368A - Feed additive - Google Patents
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- CN101053368A CN101053368A CNA200710074204XA CN200710074204A CN101053368A CN 101053368 A CN101053368 A CN 101053368A CN A200710074204X A CNA200710074204X A CN A200710074204XA CN 200710074204 A CN200710074204 A CN 200710074204A CN 101053368 A CN101053368 A CN 101053368A
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Abstract
The present invention relates to feed additive field, provides a kind of lipase feed additive. The said feed additive is mainly composed of lipase, emulsifying agent and plumleaf cratoxylum tea extract. The said feed additive of the present invention contains lipase and plumleaf cratoxylum tea extract, aiming at digesting and absorbing the fatty of feed, enhancing digestion rate of fat, promoting animal growing, reducing animal diarrhea resulting in fat indigestion, improving the survival rate of animal and feed conversion rate, especially feeding to the young animal and feeding high fat content to animal having obvious effects, it is a kind of highly effective, fully, green and environmental protection production.
Description
Technical field
The present invention relates to feed additive field, particularly a kind of lipase feed addictive.
Background technology
Functional feedstuff additive be meant have strengthen the animal body immunocompetence, regulate the organism physiology rhythm and pace of moving things, prevent disease takes place, promote the functional feed composition of body rehabilitation, it provides reliable assurance for the safety of the growing up healthy and sound of animal, livestock products.Functional feedstuff additive is generally classified non-nutritional feed additive as.In recent years along with biotechnology and engineered developing rapidly, the application of a series of feed addictives in feed such as enzyme preparation, microorganism, organic trace element and Yolk antibody are also more and more, and it has obvious effects at aspects such as promoting breeding performonce fo animals and raising animal health level.Enzyme preparation is widely used in production of fodder processing a few days ago.Enzyme is the material that is had the height catalytic activity by the class that organism produces, and claims biocatalyst again.Fodder enzyme mostly is the hydrolysis serial enzymes, as protease, lipase, cellulase, pectase, amylase, phytase etc.Lipase acts on the ester bond in the fat, and lipolysis is become aliphatic acid and glycerine.Lipase is used for improving the decomposition efficiency of fat in the feed more in the feed industry, to improve the utilization ratio that can be worth in the feed.
Early-weaned piglets is because digestive function zoon not as yet symptoms such as the disorders of digestion, diarrhoea can occur usually after wean.Therefore wherein most of trophism diarrhea that causes because of indigestion manages to improve the digestibility of piglet to nutrient (protein, fat), will be a kind of means of effective solution ablactation stress.Not only the low problem of fat digestion absorption efficiency can appear in piglet, and other animal same problem can occur the children period in age.Feed enzyme preparation commonly used on the market at present is the industrial products that contain single enzyme or mixed enzyme that process by specific production technology, but also has the low problem of feed efficiency.
Summary of the invention
The object of the present invention is to provide a kind of feed addictive, contain lipase and emulsifying agent, can improve feed efficiency.
For achieving the above object, concrete technical scheme of the present invention is: a kind of feed addictive, mainly form by lipase, emulsifying agent and Folum Ilicis extract, the usage ratio of above-mentioned three kinds of compositions is that every kilogram of feed additive contains 2000～6000 unit lipase, 0.1～0.5 gram emulsifying agent and 10～50 gram Folum Ilicis extracts, and all the other compositions are auxiliary material.
The usage ratio of described lipase, emulsifying agent and Folum Ilicis extract is that every kilogram of feed additive contains 4000 unit lipase, 0.2 gram emulsifying agent and 40 gram Folum Ilicis extracts, and all the other compositions are auxiliary material.
Described auxiliary material is a starch.
Described lipase derives from black-koji mould, rhizopus, sickle mycete, geotrichum sp, candidiasis, penicillium expansum or penicillium cyclopium.
Described emulsifying agent is the edible emulsifying agent.
Described edible emulsifying agent is glycerin monostearate, soybean lecithin, sorbitan fatty acid ester, fatty acid cane sugar ester, CSL, xylitol acid anhydride stearate or polyoxyethylene xylitol acid anhydride stearate.
Lipase activity definition: under certain experiment condition (temperature, pH), the enzyme amount of the substrate of per minute catalysis 1 μ mol is 1 unit of activity, represents with u/ml or u/g, and its unit represents with u/g in the present invention, below identical.
Feed addictive of the present invention contains lipase, at digesting and assimilating of fat in the feed, improve the digestibility of grease, promote the growth of animal, reduce the diarrhoea that animal produces because of fat indigestion, improve animal survival rate and feed conversion rate, especially the animal to the young animal and the high fat content feed of feeding has remarkable result, Folum Ilicis extract can obviously reduce oxygen radical, triglyceride and low-density lipoprotein content in the organism, changes blood viscosity.Feed addictive of the present invention be a kind of efficient, fully, the product of green, environmental protection.
The specific embodiment
(1) embodiment
Embodiment 1
Material: aspergillus niger lipase, available from Shenzhen green dimension health bioengineering Co., Ltd; Soybean lecithin is available from Shanghai Xi Run chemical industry Co., Ltd; Folum Ilicis extract is available from Yunnan Plant Pharmaceutical Industry Co., Ltd.; Starch, commercially available.
A kind of feed addictive contains 200,000 unit aspergillus niger lipase, in enzyme activity 20000U/g, needs to add 10.0 gram aspergillus niger lipase; 10.0 gram soybean lecithin, 40.0 gram Folum Ilicis extract and 49.94 kilograms of starch.
Embodiment 2
Material: rizolipase, available from Novozymes Company; Sorbitan fatty acid ester is available from Shanghai Xi Run chemical industry Co., Ltd; Folum Ilicis extract is available from Yunnan Plant Pharmaceutical Industry Co., Ltd.; Starch, commercially available.
A kind of feed addictive contains 100,000 unit rizolipases, in enzyme activity 20000U/g, needs to add 5.0 gram aspergillus niger lipase; 5.0 gram sorbitan fatty acid ester, 10 gram Folum Ilicis extract and 49.98 kilograms of starch.
Embodiment 3
Material: the mould lipase of reaping hook, available from Novozymes Company; Fatty acid cane sugar ester is available from Shanghai Xi Run chemical industry Co., Ltd; Folum Ilicis extract is available from Yunnan Plant Pharmaceutical Industry Co., Ltd.; Starch, commercially available.
A kind of feed addictive contains the mould lipase of 300,000 unit reaping hooks, in enzyme activity 20000U/g, needs to add 15.0 gram aspergillus niger lipase; 25.0 gram sorbitan fatty acid ester, 50 gram Folum Ilicis extract and 49.91 kilograms of starch.
Embodiment 4
Material: penicillium cyclopium lipase, available from Novozymes Company; Polyoxyethylene xylitol acid anhydride stearate is available from Shanghai Xi Run chemical industry Co., Ltd; Folum Ilicis extract is available from Yunnan Plant Pharmaceutical Industry Co., Ltd.; Starch, commercially available.
A kind of feed addictive contains the mould lipase of 150,000 unit reaping hooks, in enzyme activity 20000U/g, needs to add 7.5 gram aspergillus niger lipase; 12.5 gram polyoxyethylene xylitol acid anhydride stearate, 20 gram Folum Ilicis extract and 49.96 kilograms of starch.
Can also adopt the mould lipase in ground, lipase from candida sp or penicillium expansum lipase in the foregoing description, emulsifying agent can also adopt CSL or xylitol acid anhydride stearate.
(2) experimental example
Experimental example 1
With the embodiment 1 described feed addictive piglet of feeding, its effect is described.
1. materials and methods
1.1 experimental animal: 28 age in days weanling pigs, body weight is about 8 kilograms.
1.2 test material: fatty powder, commercially available.1.3 experimental design: see Table 1,128 of 28 age in days weanling pigs are selected in test for use, and are identical by genetic background, body weight is close (about 8 kilograms), is equally divided into four processing, 4 repetitions of every processing, 8 weanling pigs of every repetition, male and female half and half.
1.4 basal diet is formed and trophic level sees Table 2.
1.5 test period place feeding and management
Tested in August, 2006 and carry out on feed institute of the Chinese Academy of Agricultural Sciences scientific research pig farm, Routine Management and the vaccine program by the test pig farm carries out fully, writes down the piglet feed intake every day, and diarrhoea pig's head number and diarrhoea degree are in time treated the morbidity piglet.
Table 1 experimental design
| Control group | Handle 1 | Handle 2 | Handle 3 | |
| Every processing number | 32 | 32 | 32 | 32 |
| Every repetition number | 8 | 8 | 8 | 8 |
| Embodiment 1 feed addictive | 0 | 0.1％ | 0 | 0.1％ |
| Fatty powder | 0 | 0 | 3％ | 3％ |
| Add up to | 128 | |||
Table 2 basal diet is formed and trophic level (%)
| Daily ration is formed | Control group | Handle 1 | Handle 2 | Handle 3 |
| Fatty powder | 0 | 0 | 3 | 3 |
| Embodiment 1 feed addictive | 0 | 0.1 | 0 | 0.1 |
| Corn | 60 | 60 | 57 | 57 |
| 40% sucking pig concentrate feed | 40 | 40 | 40 | 40 |
| Add up to | 100 | 100 | 100 | 100 |
| Nutritive index | ||||
| Digestible energy (KCAL/KG) | 3300 | 3300 | 3500 | 3500 |
| Crude protein (%) | 20.2 | 20.2 | 20 | 20 |
| Calcium (%) | 0.84 | 0.84 | 0.84 | 0.84 |
| Phosphorus (%) | 0.65 | 0.65 | 0.65 | 0.65 |
| Lysine (%) | 1.25 | 1.25 | 1.25 | 1.25 |
Annotate: 40% sucking pig concentrate feed can satisfy the demand of piglet to mineral matter, trace element, vitamin, amino acid etc.
2. testing index
2.1 diarrhea rate and diarrhoea index:
Write down the piglet number of suffering from diarrhoea every day, calculate the ratio of total number of experimental period diarrhoea and test fate, piglet number, thereby calculate the grice diarrhoea rate.
Diarrhoea index scoring: with artificial scoring to grice diarrhoea serious and degree make classification, be generally 1～5 fen.
2.2 feed intake: every hurdle pig feed intake every day is accurately measured and write down to duration of test, and calculate average daily ingestion amount (ADFI).
2.3 daily gain: weighing in morning piglet is heavy on an empty stomach when the off-test of test initial sum respectively, calculates average daily gain (ADG).
2.4 feedstuff-meat ratio: the ratio (F/G) of average daily ingestion amount and average daily gain.
2.5 the death rate
3. experimental result
As shown in Table 3, compare with other three groups, add the body weight that embodiment 1 feed addictive can significantly improve test end of term piglet in the feed of fatty powder, explanation is thus added embodiment 1 feed addictive and can be promoted growth in piglets.Add the fatty powder group separately, feed efficiency is minimum, body weight is also minimum, the digestive function that piglet is described does not also have zoon, digestibility to fatty powder is still very low, although embodiment 1 feed addictive can significantly reduce the daily ingestion amount (p＜0.05) of piglet separately, yet the average daily progression rate of piglet is not made significant difference, illustrate that adding embodiment 1 feed addictive has improved the feed efficiency of piglet.After adding fatty powder separately, owing to lack lipase in the piglet alimentary canal, thereby suppressed growth in piglets, when adding fat, add embodiment 1 feed addictive, the piglet daily gain is significantly higher than other three groups, this result confirms that embodiment 1 feed addictive is a substrate with the fatty powder, has improved growth in piglets.Add the fatty powder group separately, the average daily ingestion amount of piglet is significantly higher than and adds embodiment 1 feed addictive group, and all there is not significant difference between control group, interpolation fatty powder and embodiment 1 feed addictive group (handling three), simultaneously the feed efficiency of this processed group but is minimum, therefore explanation, add fatty powder separately after, feed efficiency reduces, the increase even if search for food does not finally improve daily gain and body weight yet, and another has proved that fatty powder is difficult to the fact that is digested by piglet.
The influence (KG) of piglet production performance is handled in table 3 test
| Group | Control group | Handle 1 | Handle 2 | Handle 3 | ||||
| Number | 32 | SD | 32 | SD | 32 | SD | 32 | SD |
| Initial counterpoise | 8.15a | 0.346 | 7.84a | 0.275 | 8.19a | 0.228 | 7.993a | 0.153 |
| Finish counterpoise | 20.57a | 0.959 | 20.27a | 1.27 | 19.05a | 2.07 | 21.37b | 0.98 |
| Average daily gain | 0.496b | 0.0259 | 0.497b | 0.0415 | 0.434a | 0.0746 | 0.535c | 0.0340 |
| Average daily ingestion amount | 0.625b | 0.0558 | 0.567a | 0.0421 | 0.612b | 0.0612 | 0.609b | 0.0316 |
| Feedstuff-meat ratio | 1.30a | 0.140 | 1.14a | 0.040 | 1.43b | 0.188 | 1.14a | 0.112 |
| Diarrhea rate | 0.65％ | 0.50％ | 0.75％ | 0.50％ | ||||
Annotate: colleague's shoulder motes letter different table differential different significantly (P＜0.05)
Sum up above result, draw as drawing a conclusion: embodiment 1 feed addictive passes through to promote the digestion of fatty powder, and then has improved the production performance of piglet.
4. Economic and Efficiency Analysis
Carry out Economic and Efficiency Analysis according to current market conditions (feed and piglet price), as table 4:
Table 4
| The piglet sum | Feed price | Feed consumption rate | Feed cost | Total augment weight | The weightening finish cost | The piggy price | Weightening finish is worth | The weightening finish benefit | Average every capillus profit | Compare gross profit increases with control group | |
| Head | Unit/kg | kg | Unit | kg | Unit/kg | Unit/kg | Unit | Unit | Unit/head | Unit/head | |
| Control group | 30 | 3.2 | 469 | 1500.8 | 359.93 | 4.17 | 15 | 5398.95 | 3898.15 | 129.94 | 0 |
| Handle 1 | 30 | 3.24 | 425.5 | 1378.62 | 373.05 | 3.70 | 15 | 5595.75 | 4217.13 | 140.57 | 10.63 |
| Handle 2 | 30 | 3.37 | 459.05 | 1547.00 | 325.8 | 4.75 | 15 | 4887 | 3340.00 | 111.33 | -18.60 |
| Handle 3 | 30 | 3.41 | 457 | 1558.37 | 401.35 | 3.88 | 15 | 6020.25 | 4461.88 | 148.73 | 18.79 |
As can be seen from Table 4, aspect the piglet gross income, compare with control group, add every piglet of embodiment 1 feed addictive in the feed separately and can improve 10.63 yuan, reduced gross profit on the contrary and add fatty powder separately, every piglet gross profit has improved 18.79 yuan under the situation that embodiment 1 feed addictive and fatty powder add simultaneously.
Experimental example 2
With the embodiment 3 described feed addictives eel of feeding, its effect is described.
1 materials and methods
1.1 test material
1.1.1 embodiment 3 feed addictives
1.1.2 experiment place: the horizontal Li Manyuyangzhichang in Fanyu
1.1.3 the experimental design test is divided into control group and two processing of test group, each handles two mouthfuls of pools, is respectively: the control group 6# pool, the 8# pool, the test group 7# pool, the 9# pool.The concrete grouping as table 5:
Table 5 experimental design
| Group | Control group | Test group | ||
| The pool number | 6# | 8# | 7# | 9# |
| Fish pond area (mu) | 9 | 11 | 10 | 8 |
| The depth of water (rice) | 1.4 | 1.4 | 1.4 | 1.4 |
| Bait | Basal diet | Basal diet | Basal diet+0.2% embodiment, 3 feed addictives | Basal diet+0.2% embodiment, 3 feed addictives |
| Put mantissa's (tail) in a suitable place to breed | 30000 | 30000 | 30000 | 20000 |
| Breeding density (tail/mu) | 3333 | 2727 | 3000 | 2500 |
1.1.4 feeding and management
Regularly throw something and feed every day 2 times, the morning 05:30 and afternoon 17:30 respectively feed once, day feeding volume is calculated by 2% of eel TBW, or eats up in 15 minutes with feed and to be as the criterion, and is adjusted according to weather condition and material feeding situation.A feed table is established on every mouthful of pool, and fixed point is thrown something and fed.Feed adopts the eel material of Pu'er, Guangzhou feed corporation,Ltd, and every batch of feed formula is constant, and it is stable that quality keeps.
Observe eel material feeding situation every day, keep a close eye on the eel active situation, finds illly in time to suit the remedy to the case, regularly carry out water-quality determination in addition, observe the variation of physical and chemical index, except that the daily blowdown of strict demand with change the water, carry out water body disinfection weekly 1 time.
2 results and analysis
2.1 result of the test
In process of the test, the test group of interpolation embodiment 3 feed addictives has obviously improved the speed of growth, and the bodily form is round strong, and growth is neat, even, and the control group speed of growth obviously is not so good as test group, and the eel growth is not of uniform size.The diarrhoea phenomenon appears in the test group end, and the diarrhoea phenomenon then often appears in control group, and enteritis appears in test group eel end, and control group enteritis is more serious.Concrete data such as table 6.
Table 6 test data
| Group | Control group | Test group | ||
| The pool number | The 6# pool | The 8# pool | The 7# pool | The 9# pool |
| Eel is starting weight (gram) just | 25 | 50 | 50 | 50 |
| Total inventory (kilogram) | 1100 | 1500 | 1650 | 1350 |
| The test fate (my god) | 30 | 30 | 30 | 30 |
| Test end heavy (weight) | 50 | 85 | 95 | 110 |
Analyze analysis result such as table 7 by the test data in the table 6.
Table 7 test data analyzer
| Group | Control group | Test group | ||
| The pool number | The 6# pool | The 8# pool | The 7# pool | The 9# pool |
| Average daily gain (gram/sky) | 0.833a | 1.167b | 1.500c | 2.000d |
| Average daily ingestion amount (gram/sky. tail) | 1.222a | 1.667b | 1.833b | 2.250c |
| Feed conversion rate | 68.17％a | 70.00％a | 81.83％b | 88.89％c |
| Feed coefficient | 1.47a | 1.43a | 1.22b | 1.13b |
Annotate: colleague's shoulder motes letter identical table differential different not remarkable (P＞0.05) in the table, alphabetical different table differential different significantly (P＜0.05).
2.2 interpretation of result
In four pools, 6# pool eel seedling is starting weight and other several pools significant difference just, and the primary condition difference is not had a comparativity, and the 7# pool, the 8# pool and 9# pool primary condition are approaching, have comparativity.With the 8# pool is control group, and 7#, the 9# pool are that test group is analyzed, and analysis result is as follows:
2.2.1 average daily gain: test group has improved 28.53% and 71.38% than the average daily gain of control group respectively, and difference is extremely remarkable.
2.2.2 feed conversion rate: test group has improved 16.90% and 26.99% than the feed conversion rate of control group respectively, and difference is extremely remarkable.
2.2.3 feed coefficient: test group has reduced by 14.69% and 20.98% than the feed coefficient of control group respectively, and difference is extremely remarkable.
3 conclusions
By eel feeding experiment and test result analysis, embodiment 3 feed addictives have very big influence to the production performance of eel, it improve extremely significantly eel daily gain, improve food conversion ratio, reduced feed coefficient, reduced the diarrhea rate of eel extremely significantly, reduce eel enteritis incidence, thereby improved economic benefit significantly.Evidence, the application of embodiment 3 feed addictives on eel has significant effect.
Claims (6)
1, a kind of feed addictive, it is characterized in that it mainly is made up of lipase, emulsifying agent and Folum Ilicis extract, the usage ratio of above-mentioned three kinds of compositions is that every kilogram of feed additive contains 2000～6000 unit lipase, 0.1～0.5 gram emulsifying agent and 10～50 gram Folum Ilicis extracts, and all the other compositions are auxiliary material.
2, a kind of feed addictive according to claim 1, the usage ratio that it is characterized in that described lipase, emulsifying agent and bitter leaves bitter edible plant extract is that every kilogram of feed additive contains 4000 unit lipase, 0.2 gram emulsifying agent and 40 gram Folum Ilicis extracts, and all the other compositions are auxiliary material.
3, a kind of feed addictive according to claim 1 is characterized in that described auxiliary material is a starch.
4, a kind of feed addictive according to claim 1 is characterized in that described lipase derives from black-koji mould, rhizopus, sickle mycete, geotrichum sp, candidiasis, penicillium expansum or penicillium cyclopium.
5, a kind of feed addictive according to claim 1 is characterized in that described emulsifying agent is the edible emulsifying agent.
6, a kind of feed addictive according to claim 5 is characterized in that described edible emulsifying agent is glycerin monostearate, soybean lecithin, sorbitan fatty acid ester, fatty acid cane sugar ester, CSL, xylitol acid anhydride stearate or polyoxyethylene xylitol acid anhydride stearate.
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| Application Number | Priority Date | Filing Date | Title |
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| CNA200710074204XA CN101053368A (en) | 2007-04-25 | 2007-04-25 | Feed additive |
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| CNA200710074204XA CN101053368A (en) | 2007-04-25 | 2007-04-25 | Feed additive |
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| CN101904440A (en) * | 2010-08-02 | 2010-12-08 | 重庆威士化工有限公司 | Granulated feed modifier for improving bulkiness of granulated feed |
| CN102389027A (en) * | 2011-10-26 | 2012-03-28 | 中国农业大学 | Compound emulsifier as well as preparation method and application thereof |
| CN102860421A (en) * | 2012-02-17 | 2013-01-09 | 重庆威士化工有限公司 | Feed emulsifying agent for improving digestibility of fat and oil |
| CN102987095A (en) * | 2012-12-30 | 2013-03-27 | 青岛蔚蓝生物集团有限公司 | Compound emulsifier for livestock and poultry feed |
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| CN106562079A (en) * | 2016-09-20 | 2017-04-19 | 湖北华扬科技发展有限公司 | Enzyme-linked emulsifier and application thereof |
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| CN101904440A (en) * | 2010-08-02 | 2010-12-08 | 重庆威士化工有限公司 | Granulated feed modifier for improving bulkiness of granulated feed |
| WO2013056455A1 (en) * | 2011-10-20 | 2013-04-25 | 深圳市绿微康生物工程有限公司 | Lipase feed additive, processing method and application thereof |
| CN102389027A (en) * | 2011-10-26 | 2012-03-28 | 中国农业大学 | Compound emulsifier as well as preparation method and application thereof |
| CN103181458B (en) * | 2011-12-27 | 2014-05-14 | 杭州康德权饲料有限公司 | Grease emulsifying agent for coated feed and preparation process thereof |
| CN103181458A (en) * | 2011-12-27 | 2013-07-03 | 杭州康德权饲料有限公司 | Grease emulsifying agent for coated feed and preparation process thereof |
| CN102860421A (en) * | 2012-02-17 | 2013-01-09 | 重庆威士化工有限公司 | Feed emulsifying agent for improving digestibility of fat and oil |
| CN102987095A (en) * | 2012-12-30 | 2013-03-27 | 青岛蔚蓝生物集团有限公司 | Compound emulsifier for livestock and poultry feed |
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| CN103315169B (en) * | 2013-05-21 | 2016-02-10 | 程庆宝 | A kind of feedstuff for long-hair rabbits containing Ilex Latifolia Thunb |
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| CN107319151A (en) * | 2017-05-19 | 2017-11-07 | 东北农业大学 | It is a kind of to improve the feed addictive of sow feed nutrient digestibility and breast matter |
| CN109329588A (en) * | 2018-10-24 | 2019-02-15 | 浙江海洋大学 | Compound feed for marine fish for deep-water cage culture |
| CN109329588B (en) * | 2018-10-24 | 2022-02-08 | 浙江海洋大学 | Compound feed for marine fish for deep-water cage culture |
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