CN1262199C - Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage - Google Patents

Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage Download PDF

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CN1262199C
CN1262199C CNB2004100427198A CN200410042719A CN1262199C CN 1262199 C CN1262199 C CN 1262199C CN B2004100427198 A CNB2004100427198 A CN B2004100427198A CN 200410042719 A CN200410042719 A CN 200410042719A CN 1262199 C CN1262199 C CN 1262199C
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pspc
glossy ganoderma
polysaccharide
peptide complex
rainbow conk
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CN1600147A (en
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张日俊
王铮
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China Agricultural University
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China Agricultural University
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Abstract

The invention relates to an immunity strengthened feedstuff additive of a glycopeptide composite, a main component of the additive is a fermentation liquor of glossy ganoderma or/and coriolus versicolor. The fermentation liquor is obtained by inoculating a mycelium the glossy ganoderma or/and coriolus versicolor on a slope culture medium of an improved potato glucose agar; then adding a liquid culture medium A to prepare a shake flask seed liquor; subsequently adding a liquid culture medium B to prepare a first level fermentation seed liquor; finally adding a liquid culture medium C and deep liquid fermentating. The fermentation liquor is further processed to obtain a glycopeptide composite immunity strengthened feedstuff additive PSPc-1 (crystal elaboration product), PSPc-2 (adsorbent powder), PSPc-3 (liquid agent) and PSPc-4 (mycelium powder) of the invention respectively. The feedstuff additive would not generate residue of antibiotics, is able to promote animal growing or improve production efficiency, stimulate growth of immune systems, enhance immunity and disease resistance, and the feedstuff additive has a goode heat stability, is difficult to dead, is an innocuous, no-residue, safety, high efficiency, low cost immunity strengthened feedstuff additive of the glycopeptide composite.

Description

A kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive and its production and use
Technical field
The present invention relates to a kind of feed addictive and preparation method thereof, specifically relate to a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive of using glossy ganoderma and rainbow conk fermenting and producing and its production and use.
Background technology
Feed addictive plays a part indispensable in growth promotion, the diseases prevention of livestock and poultry cultivation and aspect such as enhance productivity.Early stage feed addictive mainly is an antibiotic, it is bringing into play important function aspect prevention of Animal diseases and the growth promotion, but scientific research finds that also antibiotic medicament residue endangers the public's health, and the outlet of China's livestock products is all brought very big negative effect.Along with the raising of people's food security consciousness, press for and use green feed additive to produce green livestock products, pollution-free food, nonreactive food or safety food.
About the research and development of green feed additive, abroad before more than 30 years, set about, and domestic just starting in recent years.In the process of exploitation antibiotics feed addictive substitute, China scientific worker has successively succeeded in developing microbe additive (probiotic), enzyme preparation, prebiotics or chemical probiotic (oligosaccharide), acidulant and middle medicinal herbs (or plant extracts) feed addictive etc., and they respectively have pluses and minuses.The weakness of enzyme preparation and probiotic is a poor heat stability, and easy inactivation is under heat-treat condition such as granulate, expanded or long-term storage all can cause inactivation.The adding proportion of acidulant big (1%～3%), cost height, destructible vitamin activity when making compound premix, and function limited (mainly adjust enteron aisle pH value, suppress some harmful microbe growth and breeding).Prebiotics or chemical probiotic mostly are the glycan class that optionally stimulates the animal intestinal beneficial bacterium to increase, as manna oligosaccharide, the few seed oligosaccharide of sucrose, fruit oligosaccharide etc., with the probiotics part that plays the same tune on different musical instruments, it is advantageous that: do not have any incompatibility when making prescription, powder, particulate material, expanded material can tolerate unfavorable factors such as all storings such as high pressure, high temperature, the extreme processing conditions of feed, so all can use.But there are the cost height in prebiotics or chemical probiotic, the drawback that effect is more weak.Chinese herbal and crude drugs preparations mostly is semifinished product, the effect instability, and some preparation remains in toxic and side effect, also has the processing cost height, a shortcoming such as resource-constrained when widely applying.
Summary of the invention
The feed addictive that the objective of the invention is to overcome prior art easily cause antibiotic residue, poor heat stability, easily inactivation, adding proportion is big, cost is high, some preparation remains the defective in toxic and side effect, particularly overcome some antibiotic and cause that the animal immune function obviously suppresses, premunition descends, cause the shortcoming of scabies secondary infection easily, thereby provide a kind of nontoxic, harmless, noresidue, safety, efficient, low-cost, can obviously promote animal immune system to grow, strengthen animal immunizing power, improve the Polysaccharide-Peptide complex immunologic facilitation feed addictive of premunition.
Another object of the present invention is to provide a kind of preparation method of described Polysaccharide-Peptide complex immunologic facilitation feed addictive.
A further object of the present invention is to provide a kind of purposes of described Polysaccharide-Peptide complex immunologic facilitation feed addictive.
The objective of the invention is to realize by the following technical solutions:
Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention, its main component is that glossy ganoderma is or/and the zymotic fluid of rainbow conk, through further back processing, obtain PSPc-1 (crystal refining product), PSPc-2 (absorption pulvis), PSPc-3 (liquor) and PSPc4 (mycelium pulvis) respectively.
Glossy ganoderma provided by the invention or/and the zymotic fluid of rainbow conk make by following step:
1) activation is cultivated on the inclined-plane: glossy ganoderma or/and the mycelium of rainbow conk is inoculated on the slant medium of improvement potato dextrose agar (PGA), was cultivated 4～10 days down at 25～32 ℃, covered with the inclined-plane until mycelium; The slant medium of described improvement potato dextrose agar (PGA) is made by following method: strip or little bulk are cut in potato 200～300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram dusty yeasts, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among the fluid nutrient medium A, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～5 days, obtain glossy ganoderma or/and the shake-flask seed liquid of rainbow conk; Described fluid nutrient medium A is w/v (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%, VB 10.002 sterilized 30 minutes for～0.005%, 121 ℃, be cooled to 25 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation is pressed 5～20% volume ratio access fluid nutrient medium B, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～7 days, obtain glossy ganoderma or/and the one grade fermemtation seed liquor of rainbow conk; Described fluid nutrient medium B is w/v (W/V) liquid mixture prepared by following material and water: glucose 0.5～1.0%, dregs of beans 2.0～4.0%, corn 5.0～10.0%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%; Or dregs of beans 1.0～2.0%, corn 5.0～10.0%, wheat bran 0.5～1.5%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 4.7H 2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby;
4) submerged fermentation: the one grade fermemtation seed liquor of step 3) preparation is pressed 5～20% volume ratio access fluid nutrient medium C,, obtain glossy ganoderma or/and the zymotic fluid of rainbow conk 25～35 ℃ of fermentations 2～7 days; Described fluid nutrient medium C is in following ratio material water w/v (W/V) liquid mixture prepared: dregs of beans 1.0～2.5% is or/and fish meal 0.5～1.0%, corn 5.0～10.0%, wheat bran 0.5～2.0%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby.
With the phenol-sulfuric acid process of routine measure this glossy ganoderma or/and the Polysaccharide-Peptide complex in the zymotic fluid of rainbow conk (polysaccharide-peptide complex, content PSPc) reaches more than the 2.0g/L.
This PSPc is differentiated: this Polysaccharide-Peptide complex odorless, no sweet taste, water imbibition is arranged, solubility is less in the cold water, can be dissolved in the hot water, and solution is faintly acid, is insoluble in the organic solvents such as ethanol; Be blue-green with solution after the hundred ketone reagent effects, be salmon pink, be aubergine, take on a red color with sulfuric acid-anhydride reaction with glacial acetic acid-strong sulfuric acid response with phenol-sulfuric acid reaction; Under concentrated sulfuric acid effect,, the purple ring occurs, stir back solution and be purple at polysaccharide solution and sulfuric acid interface with alphanaphthol reaction.
The invention provides a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1 (crystal refining product), it is with glossy ganoderma or/and the zymotic fluid of rainbow conk further concentrates and extracts, and concrete steps are as follows:
With above-mentioned glossy ganoderma or/and the centrifugal waste of the zymotic fluid of rainbow conk, get supernatant and be evaporated to 40～60% of original volume at 50～80 ℃, and then add the alcohol more than 95% of 1～3 times of volume, staticly settle after the stirring, centrifugation, recovered alcohol supernatant (can recycle), after milky fibery precipitate drying, promptly make Polysaccharide-Peptide complex (polysaccharide-peptide complex, PSPc) extract, its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1 (crystal refining product).
The invention provides another kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2 (absorption pulvis), it is with glossy ganoderma or/and the zymotic fluid of rainbow conk mixes with carrier after further concentrating, and concrete steps are as follows:
With above-mentioned glossy ganoderma or/and the zymotic fluid of rainbow conk in homogenizer behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, weight ratio=0.5～0.8 in fermentating liquid volume and mixed carrier: 1 ratio mixes, in 60～80 ℃ down dry (as fluidizing drying bed), be crushed to 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2 (absorption pulvis); Described mixed carrier (adsorbent) is the mixture by following part by weight preparation: lightweight CaCO 340～60wt%, maize cob meal 20～50wt%, corn protein powder 10～30wt%; The effective content of PSPc is not less than 4.0g/kg (absorption pulvis) in this additive.
The invention provides another Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3 (liquor), it is with glossy ganoderma or/and the zymotic fluid of rainbow conk interpolation auxiliary material makes, and concrete steps are as follows:
Or/and the zymotic fluid of rainbow conk adds the stabilizing agent of 0.1～0.5v%, described stabilizing agent is one or more the mixture that is selected from acetic acid, propionic acid, the citric acid, makes Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3 (liquor) with above-mentioned glossy ganoderma; The effective content of PSPc is not less than 3.5g/L in this additive.
The invention provides also a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4 (mycelium pulvis), make by following step:
1) the preparation glossy ganoderma is or/and the manyzoned polypore bacteria shake-flask seed liquid: be same as above-mentioned glossy ganoderma or/and the 1st in the zymotic fluid of rainbow conk) step and the 2nd) step;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into desinfection chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, thickness of feed layer 2～3cm, in be cooled to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the manyzoned polypore bacteria shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum concentration is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and corn flour 0.5～5%, sucrose 0.5～2.0%, land plaster 0.5～1.0%;
3) glossy ganoderma is or/and the back processing of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4 (mycelium pulvis).
Measure the PSPc in the mycelium: the glossy ganoderma of getting an amount of above-mentioned solid fermentation cultivation is or/and the wet thallus of prepared from coriolus versicolor mycelium, with bruisher with mycelium broken wall (confirming) through microscopy, add distilled water by 1: 20 amount, 95 ℃ of following lixiviates 3 times, each extraction time is 3h, merge 3 times leaching liquor, after concentrating 10 times through reducing pressure, 95% ethanol that adds three times of concentrate volumes, leave standstill behind the diel centrifugal, and with 75% ethanol cyclic washing sediment, do not wash out to there being reduced sugar, this sediment is thick polysaccharide.Measure the content of PSPc in this thick polysaccharide with phenol-sulfuric acid process, the result is that the glossy ganoderma cultivated of above-mentioned solid fermentation is or/and in the wet mycelium of prepared from coriolus versicolor mycelium, PSPc content is not less than 2.0g/kg, and water content is 50%, and its content of doing mycelial PSPc is not less than 4.0g/kg.
The invention provides a kind of purposes of above-mentioned Polysaccharide-Peptide complex immunologic facilitation feed addictive, amount by every kilogram of feed 5.0～10.0mg in animal feeds such as beasts, birds and aquatic products is added product of the present invention, can reach and promote growth of animal or enhance productivity, stimulating immune system is grown, and strengthens the effect of immunity and premunition.And add in the interpolation scope of 5.0～10.0mg (PSPc)/kg (feed) and use, result of use increases with the increase of addition.
Substitute antibiotic feed addictive with existing microbe additive (probiotic), enzyme preparation, prebiotics or chemical probiotic (oligosaccharide), acidulant and middle medicinal herbs (or plant extracts) etc. and compare, the advantage of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention is:
1) Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention is to utilize glossy ganoderma or/and rainbow conk mixed culture fermentation and getting contains abundant Polysaccharide-Peptide complex.Existing known, have physiological function widely as the Polysaccharide-Peptide complex of active ingredient, can the induction of immunity system produce the various kinds of cell factor, promote the generation of interferon, interleukins etc.; Activating macrophage, NK (NK) cell and T, bone-marrow-derived lymphocyte; The activating complement system; Effects such as enhancing antibody generation; That Polysaccharide-Peptide complex also has is antibiotic, antiviral, anti parasitic, antitumor, radioresistance and improve a series of effects such as breeding performonce fo animals.Thereby Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention can improve immune disease-resistance power and the production efficiency of animal.
2) glossy ganoderma and rainbow conk all are the traditional rare Chinese herbal medicines of China, contain ganoderic acid and cellulase, wherein ganoderic acid can suppress the growth of 7 kinds of pathogenic bacteria (as staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella Pneumoniae, shigella dysenteriae, bacillus ceylonensis A, salmonella typhi), keep the health of animal intestinal, cellulase can improve the utilization ratio of animal to feed; In addition, rainbow conk and glossy ganoderma also are rich in materials such as amino acid, vitamin, mineral matter and nucleosides, have health care, can improve breeding performonce fo animals.
3) the liquid deep layer fermenting legal system of utilizing provided by the invention is equipped with Polysaccharide-Peptide complex immunologic facilitation feed addictive, different with the plant extract method that adopted in the past, be not subjected to the few restriction of raw material, can carry out extensive, and composition is more stable, thereby reduces the production cost and the use cost of Polysaccharide-Peptide complex greatly.
4) the present invention utilize fungus secretion Polysaccharide-Peptide complex isoreactivity material as active ingredient, get through concentrating, extracting, do not have the weakness of the poor heat stability of enzyme preparation and probiotic.
In sum, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention is nontoxic, harmless, noresidue, safety, efficient, and be easy to industrialization, with low cost, particularly overcome the animal immune function inhibition that antibiotic causes, premunition descends, cause the shortcoming of scabies secondary infection easily, and can obviously promote animal immune system to grow, strengthen immunity and the premunition of animal.Can be widely used in premix, powder, particulate material, expanded material.
The specific embodiment
The zymotic fluid L-I of embodiment 1, preparation glossy ganoderma
1) activation is cultivated on the inclined-plane: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 4 days down at 25 ℃, cover with the inclined-plane until mycelium; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 200 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram dusty yeasts, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml fluid nutrient medium A, and cultivation is 2 days on the shaking table of 80 rev/mins of 25 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described fluid nutrient medium A is w/v (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH 2PO 40.1%, MgSO 47H 2O 0.05%, VB 1Sterilized 30 minutes for 0.002%, 121 ℃, be cooled to 25 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that fluid nutrient medium B is housed by 5% volume ratio, cultivates 2 days on the shaking table of 80 rev/mins of 25 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described fluid nutrient medium B is w/v (W/V) liquid mixture prepared by following material and water: glucose 0.5%, dregs of beans 2.0%, corn 5.0%, feed grade dusty yeast 0.5%, KH 2PO 40.1%, MgSO 47H 20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby;
4) submerged fermentation: the fermentation tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with fluid nutrient medium C by 5% volume ratio access, 25 ℃ of fermentations, measure the content of the PSPc in the zymotic fluid with phenol-sulfuric acid process, reach 2.0g/L when above up to PSPc content, stop jar, obtain the zymotic fluid L-I of glossy ganoderma; Described fluid nutrient medium C is in following ratio material water w/v (W/V) liquid mixture prepared: dregs of beans 1.0%, fish meal 0.5%, corn 5.0%, wheat bran 0.5%, feed grade dusty yeast 0.5%, KH 2PO 40.1%, MgSO 47H 20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
With the phenol-sulfuric acid process of routine measure this glossy ganoderma or/and the Polysaccharide-Peptide complex in the zymotic fluid of rainbow conk (polysaccharide-peptide complex, content PSPc) reaches more than the 2.0g/L.
This PSPc is differentiated: this Polysaccharide-Peptide complex odorless, no sweet taste, water imbibition is arranged, solubility is less in the cold water, can be dissolved in the hot water, and solution is faintly acid, is insoluble in the organic solvents such as ethanol; Be blue-green with solution after the hundred ketone reagent effects, be salmon pink, be aubergine, take on a red color with sulfuric acid-anhydride reaction with glacial acetic acid-strong sulfuric acid response with phenol-sulfuric acid reaction; Under concentrated sulfuric acid effect,, the purple ring occurs, stir back solution and be purple at polysaccharide solution and sulfuric acid interface with alphanaphthol reaction.
The zymotic fluid L-II of embodiment 2, preparation glossy ganoderma
1) activation is cultivated on the inclined-plane: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 7 days down at 27 ℃, cover with the inclined-plane until mycelium; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 250 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram dusty yeasts, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml fluid nutrient medium A, and cultivation is 3 days on the shaking table of 120 rev/mins of 27 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described fluid nutrient medium A is w/v (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH 2PO 40.15%, MgSO 47H 2O 0.075%, VB 1Sterilized 30 minutes for 0.003%, 121 ℃, be cooled to 27 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that fluid nutrient medium B is housed by 10% volume ratio, cultivates 4 days on the shaking table of 150 rev/mins of 29 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described fluid nutrient medium B is w/v (W/V) liquid mixture prepared by following material and water: glucose 0.9%, dregs of beans 3.2%, corn 7.0%, feed grade dusty yeast 1.0%, KH 2PO 40.15%, MgSO 47H 20.075%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby;
4) submerged fermentation: the fermentation tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with fluid nutrient medium C by 10% volume ratio access, 29 ℃ of fermentations, measure the content of the PSPc in the zymotic fluid with phenol-sulfuric acid process, reach 2.0g/L when above up to PSPc content, stop jar, obtain the zymotic fluid L-II of glossy ganoderma; Described fluid nutrient medium C is in following ratio material water w/v (W/V) liquid mixture prepared: dregs of beans 1.5%, corn 8.0%, wheat bran 1.5%, feed grade dusty yeast 1.0%, KH 2PO 40.15%, MgSO 47H 20.075%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby.
Use the method for embodiment 1 that this PSPc is differentiated, obtain identical result.
The zymotic fluid L-III of embodiment 3, preparation glossy ganoderma
1) activation is cultivated on the inclined-plane: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 10 days down at 32 ℃, cover with the inclined-plane until mycelium; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram dusty yeasts, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml fluid nutrient medium A, and cultivation is 5 days on the shaking table of 200 rev/mins of 35 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described fluid nutrient medium A is w/v (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH 2PO 40.2%, MgSO 47H 2O 0.1%, VB 1Sterilized 30 minutes for 0.005%, 121 ℃, be cooled to 35 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that fluid nutrient medium B is housed by 20% volume ratio, cultivates 7 days on the shaking table of 200 rev/mins of 35 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described fluid nutrient medium B is w/v (W/V) liquid mixture prepared by following material and water: glucose 1.0%, dregs of beans 4.0%, corn 10.0%, feed grade dusty yeast 1.5%, KH 2PO 40.2%, MgSO 47H 20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby;
4) submerged fermentation: the fermentation tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with fluid nutrient medium C by 20% volume ratio access, 35 ℃ of fermentations, measure the content of the PSPc in the zymotic fluid with phenol one sulfuric acid process, reach 2.0g/L when above up to PSPc content, stop jar, obtain the zymotic fluid L-III of glossy ganoderma; Described fluid nutrient medium C is in following ratio material water w/v (W/V) liquid mixture prepared: dregs of beans 2.5%, corn 10.0%, wheat bran 2.0%, feed grade dusty yeast 1.5%, KH 2PO 40.2%, MgSO 47H 20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby.
Use the method for embodiment 1 that this PSPc is differentiated, obtain identical result.
The zymotic fluid L-IV of embodiment 4, preparation glossy ganoderma
Use and embodiment 1 similar methods, the mycelium of glossy ganoderma is cultivated activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the zymotic fluid L-IV of preparation glossy ganoderma through the inclined-plane.
Fluid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is w/v (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, corn 5.0%, wheat bran 0.5%, feed grade dusty yeast 0.5%, KH 2PO 40.1%, MgSO 47H 20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
The zymotic fluid L-V of embodiment 5, preparation glossy ganoderma
Use and embodiment 1 similar methods, the mycelium of glossy ganoderma is cultivated activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the zymotic fluid L-V of preparation glossy ganoderma through the inclined-plane.
Fluid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is w/v (W/V) liquid mixture prepared by following material and water: dregs of beans 1.5%, corn 8.0%, wheat bran 1.0%, feed grade dusty yeast 1.0%, KH 2PO 40.13%, MgSO 47H 20.09%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
The zymotic fluid L-VI of embodiment 6, preparation glossy ganoderma
Use the method identical, the mycelium of glossy ganoderma is cultivated through the inclined-plane activated with embodiment 1;
Re-use 3 similar methods, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the zymotic fluid L-VI of preparation glossy ganoderma with embodiment.
Fluid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is w/v (W/V) liquid mixture prepared by following material and water: dregs of beans 2.0%, corn 10.0%, wheat bran 1.5%, feed grade dusty yeast 1.5%, KH 2PO 40.2%, MgSO 47H 20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby.
The zymotic fluid L-VII of embodiment 7, preparation glossy ganoderma
Use and embodiment 1 similar methods, the mycelium of glossy ganoderma is cultivated activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the zymotic fluid L-VII of preparation glossy ganoderma through the inclined-plane.
Fluid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is w/v (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, corn 5.0%, wheat bran 0.5%, feed grade dusty yeast 0.5%, KH 2PO 40.1%, MgSO 47H 20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
Fluid nutrient medium C when the step 4) submerged fermentation is w/v (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, fish meal 0.8%, corn 5.0%, wheat bran 0.5%, feed grade dusty yeast 0.5%, KH 2PO 40.1%%, MgSO 47H 20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
Zymotic fluid Y-I～the Y-VI of embodiment 8～13, preparation rainbow conk
Embodiment 8 uses the method identical with embodiment 1, is prepared the zymotic fluid Y-I of rainbow conk by the mycelium of rainbow conk.
Embodiment 9 uses the method identical with embodiment 2, is prepared the zymotic fluid Y-II of rainbow conk by the mycelium of rainbow conk.
Embodiment 10 uses the method identical with embodiment 3, is prepared the zymotic fluid Y-III of rainbow conk by the mycelium of rainbow conk.
Embodiment 11 uses the method identical with embodiment 4, is prepared the zymotic fluid Y-IV of rainbow conk by the mycelium of rainbow conk.
Embodiment 12 uses the method identical with embodiment 5, is prepared the zymotic fluid Y-V of rainbow conk by the mycelium of rainbow conk.
Embodiment 13 uses the method identical with embodiment 6, is prepared the zymotic fluid Y-VI of rainbow conk by the mycelium of rainbow conk.
Zymotic fluid LY-I～the LY-XI of embodiment 14～24, preparation glossy ganoderma and rainbow conk
Press preceding method, the mycelium of glossy ganoderma and rainbow conk is cultivated activation, preparation shake-flask seed liquid, also prepared one grade fermemtation seed liquor and submerged fermentation by a certain percentage through the inclined-plane, the zymotic fluid LY of preparation glossy ganoderma and rainbow conk, preparation condition is listed in table 1.
Embodiment 25, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-1 (crystal refining product)
The centrifugal waste of zymotic fluid L-I with the glossy ganoderma of embodiment 1 preparation, get supernatant and be evaporated to 40% of original volume at 50 ℃, and then add the industrial alcohol more than 95% of 1 times of volume, staticly settle after the stirring, centrifugation, recovered alcohol supernatant (can recycle), after milky fibery precipitate drying, promptly make Polysaccharide-Peptide complex (polysaccharide-peptide complex, PSPc) extract, its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-1 (crystal refining product).
The preparation of the zymotic fluid LY of table 1, glossy ganoderma and rainbow conk
Embodiment Zymotic fluid Step 1) Step 2) Step 3) Step 4)
The ratio of glossy ganoderma and rainbow conk seed liquor Fluid nutrient medium B
14 LY-I With embodiment 4 With embodiment 4 2∶1 With embodiment 4 With embodiment 4
15 LY-II With embodiment 5 With embodiment 5 3∶2 With embodiment 5 With embodiment 5
16 LY-III With embodiment 3 With embodiment 3 1.5∶1 With embodiment 3 With embodiment 3
17 LY-IV With embodiment 3 With embodiment 3 2∶1 Dregs of beans 2.0%, corn 10.0%, wheat bran 1.5%, feed grade dusty yeast 1.5%, KH 2PO 4 0.2％， MgSO 4·7H 20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby With embodiment 3
18 LY-V With embodiment 1 With embodiment 1 1∶1 With embodiment 1 With embodiment 1
19 LY-VI With embodiment 1 With embodiment 1 1∶1 With embodiment 1 With embodiment 1
20 LY-VII With embodiment 2 With embodiment 2 1∶1 With embodiment 2 With embodiment 2
21 LY-VIII With embodiment 3 With embodiment 3 1∶1 With embodiment 3 With embodiment 3
22 LY-IX With embodiment 1 With embodiment 1 1∶1 With embodiment 3 With embodiment 2
23 LY-X With embodiment 2 With embodiment 2 1∶1 With embodiment 2 With embodiment 3
24 LY-XI With embodiment 3 With embodiment 3 1∶1 With embodiment 1 With embodiment 1
Embodiment 26, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-3 (crystal refining product)
The centrifugal waste of zymotic fluid L-II with the glossy ganoderma of embodiment 2 preparation, get supernatant and be evaporated to 50% of original volume at 65 ℃, and then add the industrial alcohol more than 95% of 2 times of volumes, staticly settle centrifugation, recovered alcohol supernatant (can recycle) after the stirring, after milky fibery precipitate drying, promptly make the extract of Polysaccharide-Peptide complex (PSPc), its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-3 (crystal refining product).
Embodiment 27, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-5 (crystal refining product)
The centrifugal waste of zymotic fluid L-III with the glossy ganoderma of embodiment 3 preparation, get supernatant and be evaporated to 60% of original volume at 80 ℃, and then add the industrial alcohol more than 95% of 3 times of volumes, staticly settle centrifugation, recovered alcohol supernatant (can recycle) after the stirring, after milky fibery precipitate drying, promptly make the extract of Polysaccharide-Peptide complex (PSPc), its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-5 (crystal refining product).
Embodiment 28, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-2, PSPc-1-4, PSPc-1-6～PSPc-1-24 (crystal refining product)
According to method preparation preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1-2, PSPc-1-4, the PSPc-1-6～PSPc-1-24 (crystal refining product) of embodiment 25～27, preparation condition is listed in table 2.
The preparation of table 2, Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-1
Embodiment The zymotic fluid that uses The preparation method The feed addictive that obtains
28 Rainbow conk zymotic fluid Y-I With embodiment 25 PSPc-1-2
29 Rainbow conk zymotic fluid Y-II With embodiment 26 PSPc-1-4
30 Rainbow conk zymotic fluid Y-III With embodiment 27 PSPc-1-6
31 Ganoderma lucidum fermented liquid L-IV With embodiment 25 PSPc-1-7
32 Rainbow conk zymotic fluid Y-IV With embodiment 25 PSPc-1-8
33 The zymotic fluid LY-I of glossy ganoderma and rainbow conk With embodiment 25 PSPc-1-9
34 Ganoderma lucidum fermented liquid L-V With embodiment 25 PSPc-1-10
35 Rainbow conk zymotic fluid Y-V With embodiment 25 PSPc-1-11
36 The zymotic fluid LY-II of glossy ganoderma and rainbow conk With embodiment 25 PSPc-1-12
37 Ganoderma lucidum fermented liquid L-VI With embodiment 27 PSPc-1-13
38 The zymotic fluid L-VI of rainbow conk With embodiment 27 PSPc-1-14
39 The zymotic fluid LY-III of glossy ganoderma and rainbow conk With embodiment 27 PSPc-1-15
40 The zymotic fluid LY-IV of glossy ganoderma and rainbow conk With embodiment 27 PSPc-1-16
41 Ganoderma lucidum fermented liquid L-VII With embodiment 25 PSPc-1-17
42 The zymotic fluid LY-V of glossy ganoderma and rainbow conk With embodiment 25 PSPc-1-18
43 The zymotic fluid LY-VI of glossy ganoderma and rainbow conk With embodiment 25 PSPc-1-19
44 The zymotic fluid LY-VII of glossy ganoderma and rainbow conk With embodiment 26 PSPc-1-20
45 The zymotic fluid LY-VIII of glossy ganoderma and rainbow conk With embodiment 27 PSPc-1-21
46 The zymotic fluid LY-IX of glossy ganoderma and rainbow conk With embodiment 25 PSPc-1-22
47 The zymotic fluid LY-X of glossy ganoderma and rainbow conk With embodiment 26 PSPc-1-23
48 The zymotic fluid LY-XI of glossy ganoderma and rainbow conk With embodiment 27 PSPc-1-24
Embodiment 49～72, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2-1～PSPc-2-24 (absorption pulvis)
With above-mentioned glossy ganoderma or/and the zymotic fluid of rainbow conk in homogenizer behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, volume/weight ratio=0.5～0.8 in fermentating liquid volume and mixed carrier: 1 ratio mixes, in 60～80 ℃ down dry (as fluidizing drying bed), be crushed to 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2 (absorption pulvis); Described mixed carrier (adsorbent) is the mixture by following part by weight preparation: lightweight CaCO 340～60wt%, maize cob meal 20～50wt%, corn protein powder 10～30wt%; The effective content of PSPc is not less than 4.0g/kg (absorption pulvis) in this additive.
Concrete preparation condition such as table 3 are listed, utilize carrier absorption glossy ganoderma and/or rainbow conk zymotic fluid to prepare Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2-1～PSPc-2-24 (absorption pulvis), and the effective content of PSPc is not less than 4.0g/kg in this additive.
The preparation of table 3, Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-2
Embodiment The zymotic fluid that uses The composition of carrier and proportioning (wt%) The volume/weight ratio of zymotic fluid and mixed carrier Baking temperature (℃) The feed addictive that obtains
49 Ganoderma lucidum fermented liquid L-I Lightweight CaCO 340% maize cob meal, 30% corn protein powder 30% 0.5∶1 60 PSPc-2-1
50 Rainbow conk zymotic fluid Y-I Lightweight CaCO 350% maize cob meal, 30% corn protein powder 20% 0.6∶1 65 PSPc-2-2
51 Ganoderma lucidum fermented liquid L-II Lightweight CaCO 360% maize cob meal, 30% corn protein powder 10% 0.7∶1 70 PSPc-2-3
52 Rainbow conk zymotic fluid Y-II Lightweight CaCO 360% maize cob meal, 20% corn protein powder 20% 0.8∶1 75 PSPc-2-4
53 Ganoderma lucidum fermented liquid L-III Lightweight CaCO 350% maize cob meal, 40% corn protein powder 20% 0.5∶1 80 PSPc-2-5
54 Rainbow conk zymotic fluid Y-III Lightweight CaCO 340% maize cob meal, 50% corn protein powder 10% 0.6∶1 60 PSPc-2-6
55 Ganoderma lucidum fermented liquid L-IV With embodiment 49 0.7∶1 65 PSPc-2-7
56 Rainbow conk zymotic fluid Y-IV With embodiment 50 0.8∶1 70 PSPc-2-8
57 The zymotic fluid LY-I of glossy ganoderma and rainbow conk With embodiment 51 0.5∶1 75 PSPc-2-9
58 Ganoderma lucidum fermented liquid L-V With embodiment 52 0.6∶1 80 PSPc-2-10
59 Rainbow conk zymotic fluid Y-V With embodiment 53 0.7∶1 60 PSPc-2-11
60 The zymotic fluid LY-II of glossy ganoderma and rainbow conk With embodiment 54 0.8∶1 65 PSPc-2-12
61 Ganoderma lucidum fermented liquid L-VI With embodiment 49 0.5∶1 70 PSPc-2-13
62 Rainbow conk zymotic fluid Y-VI With embodiment 50 0.6∶1 75 PSPc-2-14
63 The zymotic fluid LY-III of glossy ganoderma and rainbow conk With embodiment 51 0.7∶1 80 PSPc-2-15
64 The zymotic fluid LY-IV of glossy ganoderma and rainbow conk With embodiment 52 0.8∶1 60 PSPc-2-16
65 Ganoderma lucidum fermented liquid L-VII With embodiment 53 0.5∶1 65 PSPc-2-17
66 The zymotic fluid LY-V of glossy ganoderma and rainbow conk With embodiment 54 0.6∶1 70 PSPc-2-18
67 The zymotic fluid LY-VI of glossy ganoderma and rainbow conk With embodiment 49 0.7∶1 75 PSPc-2-19
68 The zymotic fluid LY-VII of glossy ganoderma and rainbow conk With embodiment 50 0.8∶1 60 PSPc-2-20
69 The zymotic fluid LY-VIII of glossy ganoderma and rainbow conk With embodiment 51 0.5∶1 65 PSPc-2-21
70 The zymotic fluid LY-IX of glossy ganoderma and rainbow conk With embodiment 52 0.6∶1 70 PSPc-2-22
71 The zymotic fluid LY-X of glossy ganoderma and rainbow conk With embodiment 53 0.7∶1 75 PSPc-2-23
72 The zymotic fluid LY-XI of glossy ganoderma and rainbow conk With embodiment 54 0.8∶1 80 PSPc-2-24
Embodiment 73～96, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3-1～PSPc-3-24 (liquor)
Above-mentioned glossy ganoderma or/and the zymotic fluid of rainbow conk adds stabilizing agent-acetic acid 0.2%～0.5% and/or propionic acid 0.1%～0.5% and/or the citric acid 0.1%～0.5% of 0.1～0.5v%, is made Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3 (liquor).
Concrete preparation condition such as table 4 are listed, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3-1～PSPc-3-24 (liquor), and the effective content of PSPc is not less than 3.5g/L in this additive.
The preparation of table 4, Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-3
Embodiment The zymotic fluid that uses The composition of stabilizing agent and addition (V%) The feed addictive that obtains
73 Ganoderma lucidum fermented liquid L-I Propionic acid 0.1% citric acid 0.2% acetic acid 0.3% PSPc-3-1
74 Rainbow conk zymotic fluid Y-I Propionic acid 0.3% citric acid 0.1% acetic acid 0.5% PSPc-3-2
75 Ganoderma lucidum fermented liquid L-II Propionic acid 0.5% citric acid 0.1% PSPc-3-3
Acetic acid 0.1%
76 Rainbow conk zymotic fluid Y-II Propionic acid 0.1% citric acid 0.5% acetic acid 0.2% PSPc-3-4
77 Ganoderma lucidum fermented liquid L-III Propionic acid 0.1% citric acid 0.5% PSPc-3-5
78 Rainbow conk zymotic fluid Y-III Propionic acid 0.5% citric acid 0.1% PSPc-3-6
79 Ganoderma lucidum fermented liquid L-IV Acetic acid 0.1% citric acid 0.5% PSPc-3-7
80 Rainbow conk zymotic fluid Y-IV Acetic acid 0.5% citric acid 0.1% PSPc-3-8
81 The zymotic fluid LY-I of glossy ganoderma and rainbow conk Propionic acid 0.1% acetic acid 0.5% PSPc-3-9
82 Ganoderma lucidum fermented liquid L-V Propionic acid 0.5% acetic acid 0.1% PSPc-3-10
83 Rainbow conk zymotic fluid Y-V Acetic acid 0.1% PSPc-3-11
84 The zymotic fluid LY-II of glossy ganoderma and rainbow conk Acetic acid 0.3% PSPc-3-12
85 Ganoderma lucidum fermented liquid L-VI Acetic acid 0.5% PSPc-3-13
86 Rainbow conk zymotic fluid Y-VI Propionic acid 0.1% PSPc-3-14
87 The zymotic fluid LY-III of glossy ganoderma and rainbow conk Propionic acid 0.3% PSPc-3-15
88 The zymotic fluid LY-IV of glossy ganoderma and rainbow conk Propionic acid 0.5% PSPc-3-16
89 Ganoderma lucidum fermented liquid L-VII Citric acid 0.1% PSPc-3-17
90 The zymotic fluid LY-V of glossy ganoderma and rainbow conk Citric acid 0.3% PSPc-3-18
91 The zymotic fluid LY-VI of glossy ganoderma and rainbow conk Citric acid 0.5% PSPc-3-19
92 The zymotic fluid LY-VII of glossy ganoderma and rainbow conk Propionic acid 0.3% citric acid 0.2% acetic acid 0.3% PSPc-3-20
93 The zymotic fluid LY-VIII of glossy ganoderma and rainbow conk Propionic acid 0.1% citric acid 0.4% acetic acid 0.5% PSPc-3-21
94 The zymotic fluid LY-IX of glossy ganoderma and rainbow conk Citric acid 0.2% acetic acid 0.4% PSPc-3-22
95 The zymotic fluid LY-X of glossy ganoderma and rainbow conk Propionic acid 0.1% acetic acid 0.3% PSPc-3-23
96 The zymotic fluid LY-XI of glossy ganoderma and rainbow conk Citric acid 0.2% acetic acid 0.3% PSPc-3-24
Embodiment 97～120, preparation Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4-1～PSPc-4-24 (mycelium pulvis)
1) prepare glossy ganoderma or/and the manyzoned polypore bacteria shake-flask seed liquid: the method according to embodiment 1～24 prepares glossy ganoderma or/and the manyzoned polypore bacteria shake-flask seed liquid;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into desinfection chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, thickness of feed layer 2～3cm, in be cooled to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the manyzoned polypore bacteria shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum concentration is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and corn flour 0.5～5%, sucrose 0.5～2.0%, land plaster 0.5～1.0%;
3) glossy ganoderma is or/and the back processing of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4 (mycelium pulvis).
Concrete preparation condition such as table 5 are listed, make Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4-1～PSPc-4-24 (mycelium pulvis), and the effective content of PSPc is not less than 4.0g/kg in this additive.
The preparation of table 5, Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4
Embodiment The seed liquor of using Solid medium is formed (wt%) Seed liquor inoculum concentration (V/wt%) Cultivation temperature (℃) Incubation time (my god) The feed addictive that obtains
97 The sub-liquid of the lucidum strain of embodiment 1 The dish dregs of rice 20 wheat bran 20 cotton dregs 20 sucrose 0.5 bean cake powder 0.5 land plaster 0.5 wood chip 38 corn flour 0.5 5 25 4 PSPc-4-1
98 The manyzoned polypore bacteria seed liquor of embodiment 8 The dish dregs of rice 20 wheat bran 20 cotton dregs 20 sucrose 0.5 bean cake powder 0.5 land plaster 0.5 wood chip 38 corn flour 0.5 5 25 4 PSPc-4-2
99 The sub-liquid of the lucidum strain of embodiment 2 Cotton dregs 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 land plasters 0.5 7 27 5 PSPc-4-3
100 The manyzoned polypore bacteria seed liquor of embodiment 9 Cotton dregs 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 land plasters 0.5 7 27 5 PSPc-4-4
101 The sub-liquid of the lucidum strain of embodiment 3 The dish dregs of rice 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 land plasters 0.5 8 28 6 PSPc-4-5
102 The manyzoned polypore bacteria seed liquor of embodiment 10 The dish dregs of rice 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 land plasters 0.5 8 28 6 PSPc-4-6
103 The sub-liquid of the lucidum strain of embodiment 4 Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 corn flour 3 land plasters 0.5 10 26 7 PSPc-4-7
104 The manyzoned polypore bacteria seed liquor of embodiment 11 Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 corn flour 3 land plasters 0.5 10 26 7 PSPc-4-8
105 The glossy ganoderma bacterium of embodiment 14 and manyzoned polypore bacteria seed liquor Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 corn flour 3 land plasters 0.5 9 26 8 PSPc-4-9
106 The sub-liquid of the lucidum strain of embodiment 5 Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 corn flour 3 land plasters 0.5 9 26 8 PSPc-4-10
107 The manyzoned polypore bacteria seed liquor of embodiment 12 Bean cake powder 1.0 wheat bran 33 wood chips 60 sucrose 0.5 corn flour 5 land plasters 0.5 6 28 9 PSPc-4-11
108 The glossy ganoderma bacterium of embodiment 15 and manyzoned polypore bacteria seed liquor Bean cake powder 1.0 wheat bran 33 arts are considered 60 sucrose, 0.5 corn flour, 5 land plasters 0.5 to be worth doing 6 28 9 PSPc-4-12
109 The sub-liquid of the lucidum strain of embodiment 6 Bean cake powder 2.0 wheat bran 40 wood chips 51 sucrose 1.2 corn flours 5 land plasters 0.8 6 28 9 PSPc-4-13
110 The manyzoned polypore bacteria seed liquor of embodiment 13 Bean cake powder 2.0 wheat bran 40 wood chips 51 sucrose 1.2 corn flours 5 land plasters 0.8 6 28 9 PSPc-4-14
111 The glossy ganoderma bacterium of embodiment 16 and manyzoned polypore bacteria seed liquor Bean cake powder 1.5 wheat bran 35 wood chips 60 sucrose 1.5 corn flours 2 land plasters 1.0 10 27 10 PSPc-4-15
112 The glossy ganoderma bacterium of embodiment 17 and manyzoned polypore bacteria seed liquor Bean cake powder 1.5 wheat bran 35 wood chips 57 sucrose 1.5 corn flours 5 land plasters 1.0 10 27 10 PSPc-4-16
113 The sub-liquid of the lucidum strain of embodiment 7 Cotton dregs 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 51 land plasters 0.5 7 27 5 PSPc-4-17
114 The glossy ganoderma bacterium of embodiment 18 and manyzoned polypore bacteria seed liquor Cotton dregs 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 51 land plasters 0.5 7 27 5 PSPc-4-18
115 The glossy ganoderma bacterium of embodiment 19 and manyzoned polypore bacteria seed liquor Cotton dregs 40 wheat bran 15 bean cake powders 1.3 sucrose 2.0 wood chips 41 land plasters 0.8 8 29 8 PSPc-4-19
116 The glossy ganoderma bacterium of embodiment 20 and manyzoned polypore bacteria seed liquor Cotton dregs 40 wheat bran 15 bean cake powders 1.3 sucrose 2.0 wood chips 41 land plasters 0.8 8 29 8 PSPc-4-20
117 The glossy ganoderma bacterium of embodiment 21 and manyzoned polypore bacteria seed liquor The dish dregs of rice 30 wheat bran 15 bean cake powders 1.5 sucrose 20 wood chip land plasters 1.0 50.5 5 27 7 PSPc-4-21
118 The glossy ganoderma bacterium of embodiment 22 and manyzoned polypore bacteria seed liquor The dish dregs of rice 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chip land plasters 10 50.5 5 27 7 PSPc4-22
119 The glossy ganoderma bacterium of embodiment 23 and manyzoned polypore bacteria seed liquor The dish dregs of rice 40 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 41 land plasters 0.5 8 29 8 PSPc-4-23
120 The glossy ganoderma bacterium of embodiment 24 and manyzoned polypore bacteria seed liquor The dish dregs of rice 40 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 41 land plasters 0.5 8 29 8 PSPc-4-24
Embodiment 121, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are to the influence of laying hen premunition, production performance and egg product matter
In the laying hens essential ration as shown in table 6 of filling a prescription, add Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention, different PSPc products all makes an addition to egg approximately in the premix (being made up of trace element, vitamin etc.), the level that the content of PSPc all reaches in the table 7 to be indicated.
2500 Hainan ash test laying hens (60 ages in week, laying hen later stage) are divided into 10 groups (blank group and interpolation PSPc class group) at random, every group of 5 repetitions, each repeats 50 chickens.4 weeks of experimental period.Laying rate, egg size, dead chicken number and yolk colourity, shell thickness, the dense albumen height of each group are listed in table 7.
The feed formula of table 6, basal diet and trophic level
Feedstuff Corn Dregs of beans Wheat bran Stone flour Calcium monohydrogen phosphate Salt Vegetable oil 1% premix *
Proportioning % 58.72 26.0 2.0 84 1.5 0.34 2.0 1.0
Nutritive index Metabolizable energy (Mcal/kg) Thick protein (%) Calcium (%) Total phosphorus (%)
Trophic level 2.71 17.26 3.50 0.56
Production performance between table 7, test group and control group and egg product matter are relatively
Grouping 1 2 3 4 5 6 7 8 9 10
Performance indications Blank 0mg/kg Add PSPc-1-1 5mg/kg Add PSPc-1-2 8mg/kg Add PSPc-1-9 10mg/kg Add PSPc-2-3 5mg/kg Add PSPc-2-4 7mg/kg Add PSPc-2-16 10m/kg Add PSPc-4-7 5mg/kg Add PSPc-4-8 8mg/kg Add PSPc-4-18 10mg/kg
Egg size (g) 61.60 63.08 64.19 64.23 63.89 64.12 64.45 63.58 64.17 64.88
Dense albumen height 5.55 5.78 5.58 5.98 5.87 5.89 5.90 5.68 5.75 5.98
Yolk colourity 5.55 7.46 7.68 8.01 7.32 7.58 8.21 7.16 7.76 8.23
Eggshell strength 3.22 3.59 3.67 3.71 3.61 3.69 3.73 3.57 3.65 3.72
Shell thickness 364.12 377.04 379.87 380.13 379.45 383.55 386.63 378.44 381.43 386.87
Laying rate 82.3 85.5 86.3 86.5 85.3 85.9 86.1 85.6 86.4 86.7
Average egg weight 63.5 64.3 64.6 64.8 64.4 64.7 64.8 63.8 64.5 64.9
Feedstuff-egg ratio 2.51 2.25 2.23 2.24 2.21 2.26 2.31 2.33 2.28 2.26
Anti-city epidemic disease antibody 0.76 1.12 1.23 1.31 1.22 1.19 1.25 1.12 1.32 1.38
From the listed result of table 7 as can be seen, feed every index of different product of the present invention (PSPc-1 class, PSPc-2 class, PSPc-4 class) all significantly better than common mixed feed, particularly the antibody horizontal of yolk colourity and newcastle disease vaccine is significantly higher than control group, illustrate Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention production performance that improves laying hen and egg product matter and improve the antibody horizontal of laying hen and premunition aspect tangible good action is all arranged.
Embodiment 122, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are to the influence of fryer in production performance and immune system growth (premunition)
In the meat chick in different ages in week, add the PSPc product (seeing Table 9) of different agent, and control group does not add, detect its to immune organ grow, the influence of indexs such as antibody horizontal (premunition) and production performance.Test daily ration prescription and the trophic level that reaches see Table 8.Result of the test is listed in table 9.
Table 8, meat chick daily ration prescription and the trophic level that reaches
Material name Proportioning (%) Nutrient Trophic level
0～3 week 4～6 weeks 0～3 week 4～6 weeks
Corn 55.05 62.50 Metabolizable energy (KCal/kg) 3.10 3.10
Dregs of beans 32.00 25.35 Crude protein (%) 21.80 20.00
Wheat bran 2.30 3.00 Calcium (%) 1.05 1.00
Fish meal (import) 3.00 2.50 Available phosphorus (%) 0.51 0.45
Stone flour 0.80 0.82 Lysine (%) 1.20 1.05
Vegetable oil 3.40 3.12 Methionine (%) 0.54 0.47
Methionine 0.16 0.07 Egg+Guang (%) 0.90 0.75
Bone meal 1.47 1.32 Salt (%) 0.38 0.36
Salt 0.25 0.25
Compound premix 1.00 1.00
Annotate: per kilogram daily ration cupric 11mg, iodine 0.44mg, iron 110mg, manganese 100mg, selenium 0.25mg, zinc 120mg, cobalt 0.40mg, VA8800IU, VD 33000IU, VE22.5IU, VK 31.8mg, biotin 0.2mg, folic acid 1.0mg, niacin 50mg, pantothenic acid 11mg, riboflavin 6.6mg, thiamine 3.0mg, pyridoxol 4.4mg, VB 120.02mg, choline 1300mg.
Table 9, to 6 age in week meat chick production performance and the influence of immune organ developmental level
Grouping PSPc kind and addition (%) Body weight (g) Material anharmonic ratio (F/G) Thymus gland heavy (g/kg body weight) Spleen heavy (g/kg body weight) The bursa of farbricius heavy (g/kg body weight)
1 Control group (0mg/kg) 1302.2 2.29 1.66 1.96 2.18
2 PSPc-3-5(10mg/kg) 1418.0 2.13 1.99 2.67 2.65
3 PSPc-3-6(8mg/kg) 1415.7 2.15 1.96 2.52 2.62
4 PSPc-3-12(5mg/kg) 1408.4 2.19 1.93 2.47 2.60
5 PSPc-3-24(9mg/kg) 1413.1 2.13 1.98 2.64 2.64
6 PSPc-1-10(5mg/kg) 1410.2 2.16 1.93 2.43 2.61
7 PSPc-1-14(10mg/kg) 1419.6 2.12 1.98 2.63 2.63
8 PSPc-2-13(5mg/kg) 1411.2 2.14 1.97 2.55 2.55
9 PSPc-2-15(10mg/kg) 1421.3 2.12 2.01 2.64 2.67
10 PSPc-4-17(5mg/kg) 1415.5 2.15 1.94 2.51 2.62
11 PSPc-4-19(10mg/kg) 1417.4 2.13 1.99 2.76 2.66
Can find out from table 9, the different product of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention all can significantly improve the speed of growth of meat chick, the utilization rate of feed, and can significantly promote the growth of thymus gland, spleen and the bursa of farbricius, improving its relative weight is the immune organ index, but the growth of Polysaccharide-Peptide complex immunologic facilitation feed addictive immune stimulatory organ provided by the invention is described.And immune organ is the vitals and the material base of immunity of performance immunocyte and humoral immunity, and increasing of immune organ weight can illustrate that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention can improve the premunition of animal.In sum, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention promotes growth of meat chicken and improves premunition to be 2 the very important indexs of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention as the antibiotic substitute.
Embodiment 123, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are to the influence of the production performance and the survival rate of grass carp finishing period
The prescription of grass carp finishing period: test group is a fish meal 15%, big dregs of beans 25%, cottonseed cake 15%, rice bran 20%, rape cake 10% mixes bran 11.5%, mixed vitamin 1.0%, mixed mineral salt 2.2%, Polysaccharide-Peptide complex immunologic facilitation feed addictive PSPc-4-20 provided by the invention, 10mg/kg; Control group is not for adding Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention.Crude protein content 3 in the prescription is 2.58%.
Result of the test shows that the feed coefficient that adds the test group of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention is 1.83, and the feed coefficient of control group is 1.94, has clear improvement; The daily gain of test group improves 12.3% than control group, survival rate improves 5.7%, and the regularity of test group is good, and the death rate is low, resistance against diseases is strong, illustrates that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention all has desirable influence to the production performance and the survival rate of grass carp finishing period.
Embodiment 124, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention influence to the output of milk and mammitis incidence in the cow producing milk phase
According to the nutritional need of milk cow, formula feed, prescription sees Table 10, and the nutritional labeling and the nutrient concentration of daily ration see Table 11.60 3 tire milk cows with about 650 kilograms of body weight, be divided into 2 groups, be test group and control group, every group 30, every component is 3 repetitions, and each repeats 10, raises in the standard milk cattle farm, in the test group daily ration, added the different Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention of 10mg/kg, and control group does not add.Continuous 30 days, detect its output of milk and mammitis incidence every day, be analyzed then, the results are shown in table 12.
Table 10, the phase daily ration of giving milk are formed
The daily ration component type The feed title Control group proportioning % Test group proportioning %
Concentrated feed is formed Corn bran dregs of beans bone meal stone flour salt 1% milk cow compound premix 52 19.8 24 3.0 0.5 0.5 1.0 52 20 24 3.0 0.5 0.5 1.0
Add up to 100％ 100％
Roughage is formed Above-mentioned mixed feed corn silage sheep's hay brewex's grains 11kg 25kg 3kg 10kg 11kg 25kg 3kg 10kg
The nutritional labeling and the nutrient concentration of table 11, daily ration prescription
Project Dry (kg) Milk cow energy unit (individual) NEL (MJ) Thick protein (g) Calcium (g) Phosphorus (g) Crude fibre (g)
The nutritional labeling nutrient concentration 20.5 43.31 2.10 135.91 6.6 3244.50 15.77％ 180.2 0.88％ 125.2 0.61％ 2900.3 14.90％
Table 12, the output of milk and mammitis incidence
Grouping and processing The average output of milk (kg) Mammitis incidence (%)
1 group (control group, 0mg/kg) 32.86 5.73
2 groups (PSPc-1-3,10mg/kg) 34.76 1.19
3 groups (PSPc-1-22,10mg/kg) 34.13 1.31
4 groups (PSPc-1-23,10mg/kg) 35.03 1.50
5 groups (PSPc-2-10,10mg/kg) 34.88 1.32
6 groups (PSPc-2-11,10mg/kg) 34.35 1.33
7 groups (PSPc-2-12,10mg/kg) 35.12 1.45
8 groups (PSPc-3-7,10mg/kg) 35.08 1.52
9 groups (PSPc-3-8,10mg/kg) 34.75 1.22
10 groups (PSPc-3-21,10mg/kg) 34.82 1.43
11 groups (PSPc-4-1,10mg/kg) 35.18 1.39
12 groups (PSPc-4-2,10mg/kg) 34.54 1.32
13 groups (PSPc-4-23,10mg/kg) 35.23 1.47
From the data of table 12 as can be seen, in dairy cow diet, add dissimilar Polysaccharide-Peptide complex immunologic facilitation feedstuff additive products provided by the invention, can significantly improve the per day output of milk of milk cow, and obviously reduce the mammitis incidence of milk cow.Test shows that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention can significantly improve the per day output of milk and the premunition (immunity) of milk cow.
Embodiment 125, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are to the production performance of weanling pig and the influence of death rate
(1) experimental animal and design: choose body weight and be divided into five processing near 180 healthy of 35 age in days weanling pigs (male and female half and half), each handles 6 repetitions, and each repeats 6 pigs.In 5 weeks of experimental period, experimental design sees Table 13.
Table 13, testing program
Figure C20041004271900261
Annotate: it is basal diet that the test daily ration adopts the existing daily ration in test site, and PSPc makes an addition in the premix.
(2) feeding and management and detection index: the phase I, (43-49 days) were for raising the phase in advance, observe diarrhoea situation and incidence every day, claim the original weight of every pig in 35 days and divide circle, write down initial body weight, the average weight of each repetition and the weight of just expecting, the TBW of each repetition of weighing in 42 days, average weight and residue material amount (8 hours on an empty stomach) are calculated the average daily gain (ADG) in first week, average daily ingestion amount (ADFI) and ADG/ADFI; TBW, average weight and residue material amount (8 hours on an empty stomach) in second week (50-56 days) beginning (49 days) each repetition of weighing; In like manner, measure TBW, average weight and the residue material amount (8 hours on an empty stomach) of each repetition 56 days, 63 natural gift another names, calculate each all ADG, ADFI and ADG/ADFI.Calculate the average daily gain of phase I, average daily ingestion amount, feed efficiency (material anharmonic ratio) and morbidity death condition then.The results are shown in table 14.
Table 14, phase I average daily gain, day amount of adopting feed efficiency and death rate
Group/addition (mg/kg) Initial average weight (Kg) Average weight (kg) ADG (g) The material anharmonic ratio Average daily ingestion amount (g) A dead number of washing in a pan
The blank group 9.15±0.15 15.92±0.34 271±8 2.23±0.10 603±24 3
The aureomycin control group 8.89±0.09 17.05±0.55 326±20 1.68±0.08 547±16 1
PSPc-1-6 8.91±0.13 16.54±0.42 305±11 1.76±0.08 538±5 1
PSPc-1-7 8.99±0.15 17.40±0.61 336±21 * 1.68±0.07 566±12 * 0
PSPc-1-18 9.00±0.12 16.92±0.25 317±10 1.84±0.17 583±36 0
Notes: each has 8 pigs in repeating during initial packet, and if any the only dead naughty person of pig, average feed intake was with this pig data rejecting before then basis was eliminated in the test.Blank group of phase I whole test phase is dead altogether washes in a pan 3; The antibiotic group is extremely washed in a pan 1 altogether; PSPc-1-6 group (C group) is dead altogether washes in a pan 0; Other group does not have death.
Can find out from table 14, add average daily gain, daily ingestion amount that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention can significantly improve weanling pig, the two all is higher than antibiotic group control group, significant difference (p＜0.05), and death rate significantly is lower than control group (p＜0.01), imitates together with the antibiotic control group.Test shows that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention has tangible growth promotion effect and protection effect, has the antibiotic effect of substituting.
Embodiment 126, Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are to the influence of fryer immunity
For checking Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention in immunoenhancement result on the fryer and the application prospect in animal husbandry, test is selected for use and is liked denapon fryer 1 age in days 500 plumages, divide 5 groups, every group of 100 fryer, divide 5 repetitions, each repeats 20 chickens, feed with 5 kinds of different daily rations, be that the A group is the blank group, the B group is the antibiotic group, C organizes feed per ton and adds Polysaccharide-Peptide complex 2.35mg/kg, and D organizes feed per ton and adds Polysaccharide-Peptide complex immunologic facilitation feed addictive 7.05mg/kg provided by the invention, and E organizes feed per ton and adds Polysaccharide-Peptide complex immunologic facilitation feed addictive 11.75mg/kg provided by the invention.7 weeks of experimental period.Detecting index is blood lymphocyte increment vigor, the test of bovine serum albumin(BSA) antibody titer, the results are shown in table 15, table 16 and table 17.
Table 15, to the influence of chicken blood lymphocyte proliferation activity
Additive and concentration (mg/kg) Blank (not adding) Antibiotic contrast (enramycin, 3.2) PSPc-3-10 (2.35) PSPc-3-10 (7.05) PSPc-3-10 (11.75)
T cell viability (OD value) 2.362 2.412 2.320 2.654 2.872
As shown in Table 15, the interpolation of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention in feed can obviously strengthen the T cell viability, but T cell viability and active peptide addition are proportionate, active peptide concentration T cell viability when 11.75mg/kg is the highest, illustrates that the suitableeest addition of active peptide enhancing immunologic function is 11.75mg/kg.
Table 16, to the influence of chicken blood bovine serum albumin(BSA) antibody titer
Additive and concentration (mg/kg) Blank (not adding) Antibiotic (enramycin, 3.2) PSPc-3-10 (2.35) PSPc-3-10 (7.05) PSPc-3-10 (11.75)
Antibody titer (OD value) 1.112 1.235 1.725 3.546 6.555
As shown in Table 16, the interpolation of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention in feed can obviously strengthen the antibody titer level, and antibody titer level and Polysaccharide-Peptide complex addition are proportionate.
Table 17, meat chicken production performance result of the test
Additive and concentration (mg/kg) Blank (not adding) Antibiotic contrast (enramycin, 3.2) PSPc-3-10 (2.35) PSPc-3-10 (7.05) PSPc-3-10 (11.75)
Initial weight (g) 39.1 39.1 39.1 39.1 39.1
1-4 week
End heavy (g) 935.7 939.0 954.4 977.1 981.0
Daily gain (g) 32.02±4.13ABb 32.14±2.47ABab 32.72±3.98Aa 35.72±4.24Aa 37.13±5.34ABab
Daily ingestion amount (g) 63.89±37.07 64.36±43.52 63.45±32.91 65.02±36.05 67.43±10.13
Feedstuff-meat ratio 2.00±0.05 2.00±0.30 1.92±0.07 1.91±0.07 1.94±0.03
Dead number of elements 4 4 1 2 1
5-7 week
End heavy (g) 2352 2370 2350 2370 2374
Daily gain (g) 67.44±7.42 68.14±5.76 67.43±4.11 68.14±5.76 69.38±7.16
Daily ingestion amount (g) 162.52±18.32 162.76±37.39 159.48±41.76 162.76±37.39 162.88±43.52
Feedstuff-meat ratio 2.41±0.06 2.39±0.04 2.35±0.03 2.39±0.04 2.28±0.03
Dead number of elements 6 5 4 3 3
Annotate: A, B, p between C＜0.01, a, b, p between c＜0.05.
Can find out from table 17, in the meat chick fodder, add the heavy and efficiency of feed utilization of the daily gain that can obviously improve the meat chick, end-body of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention, and the degree that improves and the addition of Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention are proportionate, and can obviously reduce the death rate of meat chick.
Comprehensive above example illustrates that Polysaccharide-Peptide complex immunologic facilitation feed addictive provided by the invention is a kind ofly can improve livestock and poultry and aquatic livestock production performance, can strengthen the immunity enhancement type green feed additive of immunity or premunition again.

Claims (7)

1, a kind of glossy ganoderma or/and the zymotic fluid of rainbow conk make by following step:
1) activation is cultivated on the inclined-plane: glossy ganoderma or/and the mycelium of rainbow conk is inoculated on the slant medium of improvement potato dextrose agar, was cultivated 4～10 days down at 25～32 ℃, covered with the inclined-plane until mycelium;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among the fluid nutrient medium A, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～5 days, obtain glossy ganoderma or/and the shake-flask seed liquid of rainbow conk;
Described fluid nutrient medium A is the w/v liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%, VB 10.002 sterilized 30 minutes for～0.005%, 121 ℃, be cooled to 25 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation is pressed 5～20% volume ratio access fluid nutrient medium B, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～7 days, obtain glossy ganoderma or/and the one grade fermemtation seed liquor of rainbow conk;
Described fluid nutrient medium B is the w/v liquid mixture prepared by following material and water: glucose 0.5～1.0%, dregs of beans 2.0～4.0%, corn 5.0～10.0%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%; Or dregs of beans 1.0～2.0%, corn 5.0～10.0%, wheat bran 0.5～1.5%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 4.7H 2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby;
4) submerged fermentation: the one grade fermemtation seed liquor of step 3) preparation is pressed 5～20% volume ratio access fluid nutrient medium C,, obtain glossy ganoderma or/and the zymotic fluid of rainbow conk 25～35 ℃ of fermentations 2～7 days;
Described fluid nutrient medium C is in following ratio material water w/v liquid mixture prepared: dregs of beans 1.0～2.5% is or/and fish meal 0.5～1.0%, corn 5.0～10.0%, wheat bran 0.5～2.0%, feed grade dusty yeast 0.5～1.5%, KH 2PO 40.1～0.2%, MgSO 47H 2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby.
2, glossy ganoderma as claimed in claim 1 is or/and the zymotic fluid of rainbow conk, it is characterized in that, the slant medium of the improvement potato dextrose agar of described step 1) is made by following method: strip or little bulk are cut in potato 200～300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram dusty yeasts, add water then and be settled to 1000ml, packing, sterilization, standby.
3, a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive, it is with the described glossy ganoderma of claim 1 or/and the effective content of the Polysaccharide-Peptide complex that makes that the zymotic fluid of rainbow conk further concentrates and extracts is not less than the crystal refining product additive of 2.0g/L, and concrete steps are as follows:
With the described glossy ganoderma of claim 1 or/and the centrifugal waste of the zymotic fluid of rainbow conk, get supernatant and be evaporated to 40～60% of original volume at 50～80 ℃, and then add the alcohol more than 95% of 1～3 times of volume, staticly settle after the stirring, centrifugation, after milky fibery precipitate drying, make Polysaccharide-Peptide complex immunologic facilitation feed addictive.
4, a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive, it is with the described glossy ganoderma of claim 1 or/and the zymotic fluid of rainbow conk further concentrates the absorption pulvis that the effective content of the mixed Polysaccharide-Peptide complex that gets of back and carrier is not less than 4.0g/L, and concrete steps are as follows:
With the described glossy ganoderma of claim 1 or/and the zymotic fluid of rainbow conk in homogenizer behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, weight ratio=0.5～0.8 in fermentating liquid volume and mixed carrier: 1 ratio mixes, after 60～80 ℃ of following dryings, be crushed to 80 orders, obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive; Described mixed carrier is the mixture by following part by weight preparation: lightweight CaCO 340～60wt%, maize cob meal 20～50wt%, corn protein powder 10～30wt%.
5, a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive, it is with the described glossy ganoderma of claim 1 or/and the effective content of the Polysaccharide-Peptide complex that the zymotic fluid of rainbow conk interpolation auxiliary material makes is not less than the liquor additive of 3.5g/L, and concrete steps are as follows:
With the described glossy ganoderma of claim 1 or/and the zymotic fluid of rainbow conk adds percent by volume is 0.1～0.5% stabilizing agent, described stabilizing agent is one or more the mixture that is selected from acetic acid, propionic acid, the citric acid, makes Polysaccharide-Peptide complex immunologic facilitation feed addictive.
6, a kind of Polysaccharide-Peptide complex immunologic facilitation feed addictive, its effective content for dried mycelial Polysaccharide-Peptide complex is not less than the mycelium pulvis of 4.0g/L, is made by following step:
1) the preparation glossy ganoderma is or/and the manyzoned polypore bacteria shake-flask seed liquid: be same as the described glossy ganoderma of claim 1 or/and the 1st in the zymotic fluid of rainbow conk) step and the 2nd) step;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into desinfection chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, thickness of feed layer 2～3cm, in be cooled to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the manyzoned polypore bacteria shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum concentration is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and corn flour 0.5～5%, sucrose 0.5～2.0%, land plaster 0.5～1.0%;
3) glossy ganoderma is or/and the back processing of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation feed addictive.
7, a kind of claim 3,4,5 or 6 described Polysaccharide-Peptide complex immunologic facilitation feed addictives are as the purposes of the additive of beasts, birds and aquatic products animal feed.
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CN100387704C (en) * 2005-04-21 2008-05-14 中国农业大学 Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use
CN101455274B (en) * 2008-12-29 2011-05-11 江南大学 Method for producing feedstuff containing ganoderma lucidum active ingredient and feedstuff using ganoderma lucidum liquid fermentation liquid
CN102550805B (en) * 2010-12-10 2014-05-21 吉林农业大学 Chinese herbal medicine feed additive
CN105002095A (en) * 2014-04-15 2015-10-28 大自然生机股份有限公司 Composite ganoderma strain co-culture method and applications thereof
CN107779409A (en) * 2017-11-22 2018-03-09 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) A kind of sweet potato scurf bacterium fast breeding culture medium
CN113508866A (en) * 2021-03-26 2021-10-19 无锡泛宁生物科技有限公司 Production method of ganoderma lucidum functional feed additive
CN114651910A (en) * 2021-11-24 2022-06-24 无锡泛宁生物科技有限公司 Feed additive for promoting growth of broiler chickens and preparation method thereof

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