CN1600147A - Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage - Google Patents
Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage Download PDFInfo
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- CN1600147A CN1600147A CNA2004100427198A CN200410042719A CN1600147A CN 1600147 A CN1600147 A CN 1600147A CN A2004100427198 A CNA2004100427198 A CN A2004100427198A CN 200410042719 A CN200410042719 A CN 200410042719A CN 1600147 A CN1600147 A CN 1600147A
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Abstract
The invention relates to an immunity strengthened feedstuff additive of a glycopeptide composite, a main component of the additive is a fermentation liquor of glossy ganoderma or/and coriolus versicolor. The fermentation liquor is obtained by inoculating a mycelium the glossy ganoderma or/and coriolus versicolor on a slope culture medium of an improved potato glucose agar; then adding a liquid culture medium A to prepare a shake flask seed liquor; subsequently adding a liquid culture medium B to prepare a first level fermentation seed liquor; finally adding a liquid culture medium C and deep liquid fermentating. The fermentation liquor is further processed to obtain a glycopeptide composite immunity strengthened feedstuff additive PSPc-1 (crystal elaboration product), PSPc-2 (adsorbent powder), PSPc-3 (liquid agent) and PSPc-4 (mycelium powder) of the invention respectively. The feedstuff additive would not generate residue of antibiotics, is able to promote animal growing or improve production efficiency, stimulate growth of immune systems, enhance immunity and disease resistance, and the feedstuff additive has a goode heat stability, is difficult to dead, is an innocuous, no-residue, safety, high efficiency, low cost immunity strengthened feedstuff additive of the glycopeptide composite.
Description
Technical field
The present invention relates to a kind of fodder additives and preparation method thereof, specifically relate to a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives of using glossy ganoderma and rainbow conk fermentative production and its production and use.
Background technology
Fodder additives plays a part indispensable in promotes growth, the diseases prevention of livestock and poultry cultivation and aspect such as enhance productivity.Early stage fodder additives mainly is a microbiotic, it is bringing into play vital role aspect prevention of Animal diseases and the promotes growth, but scientific research finds that also antibiotic drug residue endangers the public's health, and the outlet of China's livestock product is all brought very big negative impact.Along with the raising of people's food safety consciousness, press for and use green feed additive to produce green livestock product, pollution-free food, nonreactive food or safety food.
About the research and development of green feed additive, abroad before more than 30 years, set about, and domestic just starting in recent years.In the process of exploitation antibiotics fodder additives substitute, China scientific worker has successively succeeded in developing microbe additive (probiotics), zymin, prebiotics or chemical probiotics (oligosaccharide), souring agent and middle medicinal herbs (or plant milk extract) fodder additives etc., and they respectively have relative merits.The weakness of zymin and probiotics is a poor heat stability, and easy inactivation is under heat-treat condition such as granulate, expanded or long-term storage all can cause inactivation.The adding proportion of souring agent big (1%～3%), cost height, destructible vitamin activity when making compound premix, and function limited (mainly adjust enteron aisle pH value, suppress some harmful microbe growth and breeding).Prebiotics or chemical probiotics mostly are the glycan class that optionally stimulates the animal intestinal probiotics to increase, as manna oligosaccharide, the few seed oligosaccharide of sucrose, fruit oligosaccharide etc., with the probiotics part that plays the same tune on different musical instruments, it is advantageous that: do not have any incompatibility when making prescription, powder, particulate material, expanded material can tolerate unfavorable factors such as all storings such as high pressure, high temperature, the extreme processing conditions of feed, so all can use.But there are the cost height in prebiotics or chemical probiotics, the drawback that effect is more weak.Chinese herbal and crude drugs preparations mostly is raw product, the effect instability, and some preparation remains in toxic side effect, also has the tooling cost height, a shortcoming such as resource-constrained when widely applying.
Summary of the invention
The fodder additives that the objective of the invention is to overcome prior art easily cause antibiotic remains, poor heat stability, easily inactivation, adding proportion is big, cost is high, some preparation remains the defective in toxic side effect, particularly overcome some microbiotic and cause that the animal immune function obviously suppresses, disease resistance descends, cause the shortcoming of secondary infection easily, thereby provide a kind of nontoxic, harmless, noresidue, safety, efficient, low-cost, can obviously promote animal immune system to grow, strengthen animal immunizing power, improve the Polysaccharide-Peptide complex immunologic facilitation fodder additives of disease resistance.
Another object of the present invention is to provide a kind of preparation method of described Polysaccharide-Peptide complex immunologic facilitation fodder additives.
A further object of the present invention is to provide a kind of purposes of described Polysaccharide-Peptide complex immunologic facilitation fodder additives.
The objective of the invention is to realize by the following technical solutions:
Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention, its main component is that glossy ganoderma is or/and the fermented liquid of rainbow conk, through further post-treatment, obtain PSPc-1 (crystal refining product), PSPc-2 (absorption pulvis), PSPc-3 (liquor) and PSPc-4 (mycelium pulvis) respectively.
Glossy ganoderma provided by the invention or/and the fermented liquid of rainbow conk make by following step:
1) slant culture activation: glossy ganoderma or/and the mycelium of rainbow conk is inoculated on the slant medium of improvement potato dextrose agar (PGA), was cultivated 4～10 days down at 25～32 ℃, covered with the inclined-plane until mycelium; The slant medium of described improvement potato dextrose agar (PGA) is made by following method: strip or little bulk are cut in potato 200～300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram yeast powders, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among the liquid nutrient medium A, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～5 days, obtain glossy ganoderma or/and the shake-flask seed liquid of rainbow conk; Described liquid nutrient medium A is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%, VB
10.002 sterilized 30 minutes for～0.005%, 121 ℃, be cooled to 25 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation is pressed 5～20% volume ratio access liquid nutrient medium B, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～7 days, obtain glossy ganoderma or/and the one grade fermemtation seed liquor of rainbow conk; Described liquid nutrient medium B is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 0.5～1.0%, dregs of beans 2.0～4.0%, corn 5.0～10.0%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%; Or dregs of beans 1.0～2.0%, corn 5.0～10.0%, wheat bran 0.5～1.5%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
4.7H
2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby;
4) submerged fermentation: the one grade fermemtation seed liquor of step 3) preparation is pressed 5～20% volume ratio access liquid nutrient medium C,, obtain glossy ganoderma or/and the fermented liquid of rainbow conk 25～35 ℃ of fermentations 2～7 days; Described liquid nutrient medium C is in following ratio material water weightmeasurement ratio (W/V) liquid mixture prepared: dregs of beans 1.0～2.5% is or/and fish meal 0.5～1.0%, corn 5.0～10.0%, wheat bran 0.5～2.0%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby.
With the phenolsulfuric acid method of routine measure this glossy ganoderma or/and the Polysaccharide-Peptide complex in the fermented liquid of rainbow conk (polysaccharide-peptide complex, content PSPc) reaches more than the 2.0g/L.
This PSPc is differentiated: this Polysaccharide-Peptide complex odorless, no sweet taste, water-absorbent is arranged, solubleness is less in the cold water, can be dissolved in the hot water, and solution is slightly acidic, is insoluble in the organic solvents such as ethanol; Be blue-greenish colour with solution after the hundred ketone reagent effects, be orange, be red-purple, take on a red color with sulfuric acid-anhydride reaction with Glacial acetic acid-strong sulfuric acid response with the phenolsulfuric acid reaction; Under vitriol oil effect,, the purple ring occurs, stir back solution and be purple at polysaccharide soln and sulfuric acid interface with alphanaphthol reaction.
The invention provides a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1 (crystal refining product), it is with glossy ganoderma or/and the fermented liquid of rainbow conk further concentrates and extracts, and concrete steps are as follows:
With above-mentioned glossy ganoderma or/and the centrifugal waste of the fermented liquid of rainbow conk, get supernatant liquor and be evaporated to 40～60% of original volume at 50～80 ℃, and then add the alcohol more than 95% of 1～3 times of volume, staticly settle after the stirring, centrifugation, recovered alcohol supernatant liquor (can recycle), after oyster white fibery precipitate drying, promptly make Polysaccharide-Peptide complex (polysaccharide-peptide complex, PSPc) extract, its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1 (crystal refining product).
The invention provides another kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2 (absorption pulvis), it is with glossy ganoderma or/and the fermented liquid of rainbow conk mixes with carrier after further concentrating, and concrete steps are as follows:
With above-mentioned glossy ganoderma or/and the fermented liquid of rainbow conk in clarifixator behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, press weight ratio=0.5～0.8 of fermentating liquid volume and mixed carrier: 1 mixed is even, in 60～80 ℃ down dry (as fluidizing drying bed), be crushed to 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2 (absorption pulvis); Described mixed carrier (sorbent material) is the mixture by following part by weight preparation: lightweight CaCO
340～60wt%, corn cob meal 20～50wt%, Zein powder 10～30wt%; The effective content of PSPc is not less than 4.0g/kg (absorption pulvis) in this additive.
The invention provides another Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3 (liquor), it is with glossy ganoderma or/and the fermented liquid of rainbow conk interpolation auxiliary material makes, and concrete steps are as follows:
Or/and the fermented liquid of rainbow conk adds the stablizer of 0.1～0.5v%, described stablizer is one or more the mixture that is selected from acetic acid, propionic acid, the citric acid, makes Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3 (liquor) with above-mentioned glossy ganoderma; The effective content of PSPc is not less than 3.5g/L in this additive.
The invention provides also a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4 (mycelium pulvis), make by following step:
1) the preparation glossy ganoderma is or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid: be same as above-mentioned glossy ganoderma or/and the 1st in the fermented liquid of rainbow conk) step and the 2nd) step;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into sterilisable chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, bed thickness 2～3cm, internal cooling to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum size is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and Semen Maydis powder 0.5～5%, sucrose 0.5～2.0%, terra alba 0.5～1.0%;
3) glossy ganoderma is or/and the post-treatment of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4 (mycelium pulvis).
Measure the PSPc in the mycelium: the glossy ganoderma of getting an amount of above-mentioned solid fermentation cultivation is or/and the wet thallus of prepared from coriolus versicolor mycelium, with stamp mill with mycelium broken wall (confirming) through microscopy, add distilled water by 1: 20 amount, 95 ℃ of following lixiviates 3 times, each extraction time is 3h, merge 3 times vat liquor, behind 10 times of concentrating under reduced pressure, 95% ethanol that adds three times of concentrated solution volumes, leave standstill behind the diel centrifugal, and with 75% ethanol repetitive scrubbing throw out, do not wash out to there being reducing sugar, this throw out is Crude polysaccharides.Measure the content of PSPc in this Crude polysaccharides with the phenolsulfuric acid method, the result is that the glossy ganoderma cultivated of above-mentioned solid fermentation is or/and in the wet mycelium of prepared from coriolus versicolor mycelium, PSPc content is not less than 2.0g/kg, and water content is 50%, and its content of doing mycelial PSPc is not less than 4.0g/kg.
The invention provides a kind of purposes of above-mentioned Polysaccharide-Peptide complex immunologic facilitation fodder additives, amount by every kilogram of feed 5.0～10.0mg in animal-feeds such as beasts, birds and aquatic products is added product of the present invention, can reach and promote growth of animal or enhance productivity, stimulating immune system is grown, the effect of strengthening immunity and disease resistance.And add in the interpolation scope of 5.0～10.0mg (PSPc)/kg (feed) and use, result of use increases with the increase of addition.
Substitute antibiotic fodder additives with existing microbe additive (probiotics), zymin, prebiotics or chemical probiotics (oligosaccharide), souring agent and middle medicinal herbs (or plant milk extract) etc. and compare, the advantage of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention is:
1) Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention is to utilize glossy ganoderma or/and rainbow conk mixed fermentation and getting contains abundant Polysaccharide-Peptide complex.Existing known, have physiological function widely as the Polysaccharide-Peptide complex of effective constituent, can the induction of immunity system produce the various kinds of cell factor, promote the generation of Interferon, rabbit, interleukin-etc.; Activating macrophage, NK cell (NK) cell and T, bone-marrow-derived lymphocyte; The activating complement system; Effects such as enhancing antibody generation; That Polysaccharide-Peptide complex also has is antibiotic, antiviral, parasiticide, antitumor, radioprotective and improve a series of effects such as breeding performonce fo animals.Thereby Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention can improve immune disease-resistance power and the production efficiency of animal.
2) glossy ganoderma and rainbow conk all are the traditional rare Chinese herbal medicines of China, contain Ganodenic acid and cellulase, wherein Ganodenic acid can suppress the growth of 7 kinds of pathogenic bacterium (as streptococcus aureus, intestinal bacteria, Pseudomonas aeruginosa, Klebsiella Pneumoniae, shigella dysenteriae, bacillus ceylonensis A, salmonella typhi), keep the health of animal intestinal, cellulase can improve the utilising efficiency of animal to feed; In addition, rainbow conk and glossy ganoderma also are rich in materials such as amino acid, VITAMIN, mineral substance and nucleosides, have nourishing function, can improve breeding performonce fo animals.
3) the liquid submerged fermentation legal system of utilizing provided by the invention is equipped with Polysaccharide-Peptide complex immunologic facilitation fodder additives, different with the plant extract method that adopted in the past, be not subjected to the few restriction of raw material, can carry out extensive, and composition is more stable, thereby reduces the production cost and the use cost of Polysaccharide-Peptide complex greatly.
4) the present invention utilize fungus secretion Polysaccharide-Peptide complex isoreactivity material as effective constituent, get through concentrating, extracting, do not have the weakness of the poor heat stability of zymin and probiotics.
In sum, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention is nontoxic, harmless, noresidue, safety, efficient, and be easy to industrialization, with low cost, particularly overcome the animal immune function inhibition that microbiotic causes, disease resistance descends, cause the shortcoming of secondary infection easily, and can obviously promote animal immune system to grow, strengthen immunizing power and the disease resistance of animal.Can be widely used in Preblend, powder, particulate material, expanded material.
Embodiment
The fermented liquid L-I of embodiment 1, preparation glossy ganoderma
1) slant culture activation: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 4 days down, cover with the inclined-plane until mycelium at 25 ℃; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 200 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram yeast powders, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml liquid nutrient medium A, and cultivation is 2 days on the shaking table of 80 rev/mins of 25 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described liquid nutrient medium A is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH
2PO
40.1%, MgSO
47H
2O 0.05%, VB
1Sterilized 30 minutes for 0.002%, 121 ℃, be cooled to 25 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that liquid nutrient medium B is housed by 5% volume ratio, cultivates 2 days on the shaking table of 80 rev/mins of 25 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described liquid nutrient medium B is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 0.5%, dregs of beans 2.0%, corn 5.0%, feed grade yeast powder 0.5%, KH
2PO
40.1%, MgSO
47H
20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby;
4) submerged fermentation: the fermentor tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with liquid nutrient medium C by 5% volume ratio access, 25 ℃ of fermentations, measure the content of the PSPc in the fermented liquid with the phenolsulfuric acid method, reach 2.0g/L when above up to PSPc content, stop jar, obtain the fermented liquid L-I of glossy ganoderma; Described liquid nutrient medium C is in following ratio material water weightmeasurement ratio (W/V) liquid mixture prepared: dregs of beans 1.0%, fish meal 0.5%, corn 5.0%, wheat bran 0.5%, feed grade yeast powder 0.5%, KH
2PO
40.1%, MgSO
47H
20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
With the phenolsulfuric acid method of routine measure this glossy ganoderma or/and the Polysaccharide-Peptide complex in the fermented liquid of rainbow conk (polysaccharide-peptide complex, content PSPc) reaches more than the 2.0g/L.
This PSPc is differentiated: this Polysaccharide-Peptide complex odorless, no sweet taste, water-absorbent is arranged, solubleness is less in the cold water, can be dissolved in the hot water, and solution is slightly acidic, is insoluble in the organic solvents such as ethanol; Be blue-greenish colour with solution after the hundred ketone reagent effects, be orange, be red-purple, take on a red color with sulfuric acid-anhydride reaction with Glacial acetic acid-strong sulfuric acid response with the phenolsulfuric acid reaction; Under vitriol oil effect,, the purple ring occurs, stir back solution and be purple at polysaccharide soln and sulfuric acid interface with alphanaphthol reaction.
The fermented liquid L-II of embodiment 2, preparation glossy ganoderma
1) slant culture activation: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 7 days down, cover with the inclined-plane until mycelium at 27 ℃; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 250 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram yeast powders, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml liquid nutrient medium A, and cultivation is 3 days on the shaking table of 120 rev/mins of 27 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described liquid nutrient medium A is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH
2PO
40.15%, MgSO
47H
2O 0.075%, VB
1Sterilized 30 minutes for 0.003%, 121 ℃, be cooled to 27 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that liquid nutrient medium B is housed by 10% volume ratio, cultivates 4 days on the shaking table of 150 rev/mins of 29 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described liquid nutrient medium B is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 0.9%, dregs of beans 3.2%, corn 7.0%, feed grade yeast powder 1.0%, KH
2PO
40.15%, MgSO
4.7H
20.075%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby;
4) submerged fermentation: the fermentor tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with liquid nutrient medium C by 10% volume ratio access, 29 ℃ of fermentations, measure the content of the PSPc in the fermented liquid with the phenolsulfuric acid method, reach 2.0g/L when above up to PSPc content, stop jar, obtain the fermented liquid L-II of glossy ganoderma; Described liquid nutrient medium C is in following ratio material water weightmeasurement ratio (W/V) liquid mixture prepared: dregs of beans 1.5%, corn 8.0%, wheat bran 1.5%, feed grade yeast powder 1.0%, KH
2PO
40.15%, MgSO
47H
20.075%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby.
Use the method for embodiment 1 that this PSPc is differentiated, obtain identical result.
The fermented liquid L-III of embodiment 3, preparation glossy ganoderma
1) slant culture activation: in the 15ml test tube, the mycelium of glossy ganoderma is inoculated on the slant medium of improvement potato dextrose agar (PGA), cultivated 10 days down, cover with the inclined-plane until mycelium at 32 ℃; The concrete method for making of the slant medium of described improvement potato dextrose agar (PGA) is: strip or little bulk are cut in potato 300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram yeast powders, add water then and be settled to 1000ml, packing, sterilization, standby;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among about 100ml liquid nutrient medium A, and cultivation is 5 days on the shaking table of 200 rev/mins of 35 ℃, rotating speed, obtains the shake-flask seed liquid of glossy ganoderma; Described liquid nutrient medium A is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH
2PO
40.2%, MgSO
47H
2O 0.1%, VB
1Sterilized 30 minutes for 0.005%, 121 ℃, be cooled to 35 ℃, standby;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation inserts the seed fermentation jar that liquid nutrient medium B is housed by 20% volume ratio, cultivates 7 days on the shaking table of 200 rev/mins of 35 ℃, rotating speed, obtains the one grade fermemtation seed liquor of glossy ganoderma; Described liquid nutrient medium B is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: glucose 1.0%, dregs of beans 4.0%, corn 10.0%, feed grade yeast powder 1.5%, KH
2PO
40.2%, MgSO
47H
20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 29 ℃, and is standby;
4) submerged fermentation: the fermentor tank that the one grade fermemtation seed liquor of step 3) preparation is equipped with liquid nutrient medium C by 20% volume ratio access, 35 ℃ of fermentations, measure the content of the PSPc in the fermented liquid with the phenolsulfuric acid method, reach 2.0g/L when above up to PSPc content, stop jar, obtain the fermented liquid L-III of glossy ganoderma; Described liquid nutrient medium C is in following ratio material water weightmeasurement ratio (W/V) liquid mixture prepared: dregs of beans 2.5%, corn 10.0%, wheat bran 2.0%, feed grade yeast powder 1.5%, KH
2PO
40.2%, MgSO
47H
20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby.
Use the method for embodiment 1 that this PSPc is differentiated, obtain identical result.
The fermented liquid L-IV of embodiment 4, preparation glossy ganoderma
Use and embodiment 1 similar methods, with the mycelium of glossy ganoderma through slant culture activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the fermented liquid L-IV of preparation glossy ganoderma.
Liquid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, corn 5.0%, wheat bran 0.5%, feed grade yeast powder 0.5%, KH
2PO
40.1%, MgSO
47H
20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
The fermented liquid L-V of embodiment 5, preparation glossy ganoderma
Use and embodiment 1 similar methods, with the mycelium of glossy ganoderma through slant culture activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the fermented liquid L-V of preparation glossy ganoderma.
Liquid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: dregs of beans 1.5%, corn 8.0%, wheat bran 1.0%, feed grade yeast powder 1.0%, KH
2PO
40.13%, MgSO
47H
20.09%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
The fermented liquid L-VI of embodiment 6, preparation glossy ganoderma
Use the method identical, the mycelium of glossy ganoderma is activated through slant culture with embodiment 1;
Re-use 3 similar methods, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the fermented liquid L-VI of preparation glossy ganoderma with embodiment.
Liquid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: dregs of beans 2.0%, corn 10.0%, wheat bran 1.5%, feed grade yeast powder 1.5%, KH
2PO
40.2%, MgSO
47H
20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby.
The fermented liquid L-VII of embodiment 7, preparation glossy ganoderma
Use and embodiment 1 similar methods, with the mycelium of glossy ganoderma through slant culture activation, preparation shake-flask seed liquid, preparation one grade fermemtation seed liquor and submerged fermentation, the fermented liquid L-VII of preparation glossy ganoderma.
Liquid nutrient medium B when step 3) prepares the one grade fermemtation seed liquor is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, corn 5.0%, wheat bran 0.5%, feed grade yeast powder 0.5%, KH
2PO
40.1%, MgSO
47H
20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
Liquid nutrient medium C when the step 4) submerged fermentation is weightmeasurement ratio (W/V) liquid mixture prepared by following material and water: dregs of beans 1.0%, fish meal 0.8%, corn 5.0%, wheat bran 0.5%, feed grade yeast powder 0.5%, KH
2PO
40.1%%, MgSO
47H
20.05%, 121 ℃ of sterilization of O 30 minutes is cooled to 25 ℃, and is standby.
Fermented liquid Y-I～the Y-VI of embodiment 8～13, preparation rainbow conk
Embodiment 8 uses the method identical with embodiment 1, is prepared the fermented liquid Y-I of rainbow conk by the mycelium of rainbow conk.
Embodiment 9 uses the method identical with embodiment 2, is prepared the fermented liquid Y-II of rainbow conk by the mycelium of rainbow conk.
Embodiment 10 uses the method identical with embodiment 3, is prepared the fermented liquid Y-III of rainbow conk by the mycelium of rainbow conk.
Embodiment 11 uses the method identical with embodiment 4, is prepared the fermented liquid Y-IV of rainbow conk by the mycelium of rainbow conk.
Embodiment 12 uses the method identical with embodiment 5, is prepared the fermented liquid Y-V of rainbow conk by the mycelium of rainbow conk.
Embodiment 13 uses the method identical with embodiment 6, is prepared the fermented liquid Y-VI of rainbow conk by the mycelium of rainbow conk.
Fermented liquid LY-I～the LY-XI of embodiment 14～24, preparation glossy ganoderma and rainbow conk
Press preceding method, with the mycelium of glossy ganoderma and rainbow conk through slant culture activation, preparation shake-flask seed liquid, and prepare one grade fermemtation seed liquor and submerged fermentation by a certain percentage, the fermented liquid LY of preparation glossy ganoderma and rainbow conk, preparation condition is listed in table 1.
Embodiment 25, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-1 (crystal refining product)
The centrifugal waste of fermented liquid L-I with the glossy ganoderma of embodiment 1 preparation, get supernatant liquor and be evaporated to 40% of original volume at 50 ℃, and then add the industrial spirit more than 95% of 1 times of volume, staticly settle after the stirring, centrifugation, recovered alcohol supernatant liquor (can recycle), after oyster white fibery precipitate drying, promptly make Polysaccharide-Peptide complex (polysaccharide-peptide complex, PSPc) extract, its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-1 (crystal refining product).
The preparation of the fermented liquid LY of table 1, glossy ganoderma and rainbow conk
| Embodiment | Fermented liquid | Step 1) | Step 2) | Step 3) | Step 4) | |
| The ratio of glossy ganoderma and rainbow conk seed liquor | Liquid nutrient medium B | |||||
| ??14 | ????LY-I | With embodiment 4 | With embodiment 4 | ?????2∶1 | With embodiment 4 | With embodiment 4 |
| ??15 | ????LY-II | With embodiment 5 | With embodiment 5 | ?????3∶2 | With embodiment 5 | With embodiment 5 |
| ??16 | ????LY-III | With embodiment 3 | With embodiment 3 | ????1.5∶1 | With embodiment 3 | With embodiment 3 |
| ??17 | ????LY-IV | With embodiment 3 | With embodiment 3 | ?????2∶1 | Dregs of beans 2.0%, corn 10.0%, wheat bran 1.5%, feed grade yeast powder 1.5%, KH 2PO 4?0.2％， ?MgSO 4·7H 20.1%, 121 ℃ of sterilization of O 30 minutes is cooled to 35 ℃, and is standby | With embodiment 3 |
| ??18 | ????LY-V | With embodiment 1 | With embodiment 1 | ?????1∶1 | With embodiment 1 | With embodiment 1 |
| ??19 | ????LY-VI | With embodiment 1 | With embodiment 1 | ?????1∶1 | With embodiment 1 | With embodiment 1 |
| ??20 | ????LY-VII | With embodiment 2 | With embodiment 2 | ?????1∶1 | With embodiment 2 | With embodiment 2 |
| ??21 | ????LY-VIII | With embodiment 3 | With embodiment 3 | ?????1∶1 | With embodiment 3 | With embodiment 3 |
| ??22 | ????LY-IX | With embodiment 1 | With embodiment 1 | ?????1∶1 | With embodiment 3 | With embodiment 2 |
| ??23 | ????LY-X | With embodiment 2 | With embodiment 2 | ?????1∶1 | With embodiment 2 | With embodiment 3 |
| ??24 | ????LY-XI | With embodiment 3 | With embodiment 3 | ?????1∶1 | With embodiment 1 | With embodiment 1 |
Embodiment 26, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-3 (crystal refining product)
The centrifugal waste of fermented liquid L-II with the glossy ganoderma of embodiment 2 preparation, get supernatant liquor and be evaporated to 50% of original volume at 65 ℃, and then add the industrial spirit more than 95% of 2 times of volumes, staticly settle centrifugation, recovered alcohol supernatant liquor (can recycle) after the stirring, after oyster white fibery precipitate drying, promptly make the extract of Polysaccharide-Peptide complex (PSPc), its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-3 (crystal refining product).
Embodiment 27, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-5 (crystal refining product)
The centrifugal waste of fermented liquid L-III with the glossy ganoderma of embodiment 3 preparation, get supernatant liquor and be evaporated to 60% of original volume at 80 ℃, and then add the industrial spirit more than 95% of 3 times of volumes, staticly settle centrifugation, recovered alcohol supernatant liquor (can recycle) after the stirring, after oyster white fibery precipitate drying, promptly make the extract of Polysaccharide-Peptide complex (PSPc), its purity reaches more than 95%, is Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-5 (crystal refining product).
Embodiment 28, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-2, PSPc-1-4, PSPc-1-6～PSPc-1-24 (crystal refining product)
According to method preparation preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1-2, PSPc-1-4, the PSPc-1-6～PSPc-1-24 (crystal refining product) of embodiment 25～27, preparation condition is listed in table 2.
The preparation of table 2, Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-1
| Embodiment | The fermented liquid that uses | The preparation method | The fodder additives that obtains |
| ???28 | Rainbow conk fermented liquid Y-I | With embodiment 25 | ?????PSPc-1-2 |
| ???29 | Rainbow conk fermented liquid Y-II | With embodiment 26 | ?????PSPc-1-4 |
| ???30 | Rainbow conk fermented liquid Y-III | With embodiment 27 | ?????PSPc-1-6 |
| ???31 | Ganoderma fermentation liquid L-IV | With embodiment 25 | ?????PSPc-1-7 |
| ???32 | Rainbow conk fermented liquid Y-IV | With embodiment 25 | ?????PSPc-1-8 |
| ???33 | The fermented liquid LY-I of glossy ganoderma and rainbow conk | With embodiment 25 | ?????PSPc-1-9 |
| ???34 | Ganoderma fermentation liquid L-V | With embodiment 25 | ?????PSPc-1-10 |
| ???35 | Rainbow conk fermented liquid Y-V | With embodiment 25 | ?????PSPc-1-11 |
| ???36 | The fermented liquid LY-II of glossy ganoderma and rainbow conk | With embodiment 25 | ?????PSPc-1-12 |
| ???37 | Ganoderma fermentation liquid L-VI | With embodiment 27 | ?????PSPc-1-13 |
| ???38 | The fermented liquid L-VI of rainbow conk | With embodiment 27 | ?????PSPc-1-14 |
| ???39 | The fermented liquid LY-III of glossy ganoderma and rainbow conk | With embodiment 27 | ?????PSPc-1-15 |
| ???40 | The fermented liquid LY-IV of glossy ganoderma and rainbow conk | With embodiment 27 | ?????PSPc-1-16 |
| ???41 | Ganoderma fermentation liquid L-VII | With embodiment 25 | ?????PSPc-1-17 |
| ???42 | The fermented liquid LY-V of glossy ganoderma and rainbow conk | With embodiment 25 | ????PSPc-1-18 |
| ???43 | The fermented liquid LY-VI of glossy ganoderma and rainbow conk | With embodiment 25 | ????PSPc-1-19 |
| ???44 | The fermented liquid LY-VII of glossy ganoderma and rainbow conk | With embodiment 26 | ????PSPc-1-20 |
| ???45 | The fermented liquid LY-VIII of glossy ganoderma and rainbow conk | With embodiment 27 | ????PSPc-1-21 |
| ???46 | The fermented liquid LY-IX of glossy ganoderma and rainbow conk | With embodiment 25 | ????PSPc-1-22 |
| ???47 | The fermented liquid LY-X of glossy ganoderma and rainbow conk | With embodiment 26 | ????PSPc-1-23 |
| ???48 | The fermented liquid LY-XI of glossy ganoderma and rainbow conk | With embodiment 27 | ????PSPc-1-24 |
Embodiment 49～72, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2-1～PSPc-2-24 (absorption pulvis)
With above-mentioned glossy ganoderma or/and the fermented liquid of rainbow conk in clarifixator behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, press volume/weight ratio=0.5～0.8 of fermentating liquid volume and mixed carrier: 1 mixed is even, in 60～80 ℃ down dry (as fluidizing drying bed), be crushed to 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2 (absorption pulvis); Described mixed carrier (sorbent material) is the mixture by following part by weight preparation: lightweight CaCO
340～60wt%, corn cob meal 20～50wt%, Zein powder 10～30wt%; The effective content of PSPc is not less than 4.0g/kg (absorption pulvis) in this additive.
Concrete preparation condition such as table 3 are listed, utilize carrier absorption glossy ganoderma and/or rainbow conk fermented liquid to prepare Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2-1～PSPc-2-24 (absorption pulvis), and the effective content of PSPc is not less than 4.0g/kg in this additive.
The preparation of table 3, Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-2
| Embodiment | The fermented liquid that uses | The composition of carrier and proportioning (wt%) | The volume/weight ratio of fermented liquid and mixed carrier | Drying temperature (℃) | The fodder additives that obtains |
| ???49 | Ganoderma fermentation liquid L-I | Lightweight CaCO 340% corn cob meal, 30% Zein powder 30% | ????0.5∶1 | ???60 | ???PSPc-2-1 |
| ???50 | Rainbow conk fermented liquid Y-I | Lightweight CaCO 350% corn cob meal, 30% Zein powder 20% | ????0.6∶1 | ???65 | ???PSPc-2-2 |
| ???51 | Ganoderma fermentation liquid L-II | Lightweight CaCO 360% corn cob meal, 30% Zein powder 10% | ????0.7∶1 | ???70 | ???PSPc-2-3 |
| ???52 | Rainbow conk fermented liquid Y-II | Lightweight CaCO 360% corn cob meal, 20% Zein powder 20% | ????0.8∶1 | ???75 | ???PSPc-2-4 |
| ??53 | Ganoderma fermentation liquid L-III | Lightweight CaCO 350% corn cob meal, 40% Zein powder 20% | ????0.5∶1 | ????80 | ????PSPc-2-5 |
| ??54 | Rainbow conk fermented liquid Y-III | Lightweight CaCO 340% corn cob meal, 50% Zein powder 10% | ????0.6∶1 | ????60 | ????PSPc-2-6 |
| ??55 | Ganoderma fermentation liquid L-IV | With embodiment 49 | ????0.7∶1 | ????65 | ????PSPc-2-7 |
| ??56 | Rainbow conk fermented liquid Y-IV | With embodiment 50 | ????0.8∶1 | ????70 | ????PSPc-2-8 |
| ??57 | The fermented liquid LY-I of glossy ganoderma and rainbow conk | With embodiment 51 | ????0.5∶1 | ????75 | ????PSPc-2-9 |
| ??58 | Ganoderma fermentation liquid L-V | With embodiment 52 | ????0.6∶1 | ????80 | ????PSPc-2-10 |
| ??59 | Rainbow conk fermented liquid Y-V | With embodiment 53 | ????0.7∶1 | ????60 | ????PSPc-2-11 |
| ??60 | The fermented liquid LY-II of glossy ganoderma and rainbow conk | With embodiment 54 | ????0.8∶1 | ????65 | ????PSPc-2-12 |
| ??61 | Ganoderma fermentation liquid L-VI | With embodiment 49 | ????0.5∶1 | ????70 | ????PSPc-2-13 |
| ??62 | Rainbow conk fermented liquid Y-VI | With embodiment 50 | ????0.6∶1 | ????75 | ????PSPc-2-14 |
| ??63 | The fermented liquid LY-III of glossy ganoderma and rainbow conk | With embodiment 51 | ????0.7∶1 | ????80 | ????PSPc-2-15 |
| ??64 | The fermented liquid LY-IV of glossy ganoderma and rainbow conk | With embodiment 52 | ????0.8∶1 | ????60 | ????PSPc-2-16 |
| ??65 | Ganoderma fermentation liquid L-VII | With embodiment 53 | ????0.5∶1 | ????65 | ????PSPc-2-17 |
| ??66 | The fermented liquid LY-V of glossy ganoderma and rainbow conk | With embodiment 54 | ????0.6∶1 | ????70 | ????PSPc-2-18 |
| ??67 | The fermented liquid LY-VI of glossy ganoderma and rainbow conk | With embodiment 49 | ????0.7∶1 | ????75 | ????PSPc-2-19 |
| ??68 | The fermented liquid LY-VII of glossy ganoderma and rainbow conk | With embodiment 50 | ????0.8∶1 | ????60 | ????PSPc-2-20 |
| ??69 | The fermented liquid LY-VIII of glossy ganoderma and rainbow conk | With embodiment 51 | ????0.5∶1 | ????65 | ????PSPc-2-21 |
| ??70 | The fermented liquid LY-IX of glossy ganoderma and rainbow conk | With embodiment 52 | ????0.6∶1 | ????70 | ????PSPc-2-22 |
| ??71 | The fermented liquid LY-X of glossy ganoderma and rainbow conk | With embodiment 53 | ????0.7∶1 | ????75 | ????PSPc-2-23 |
| ??72 | The fermented liquid LY-XI of glossy ganoderma and rainbow conk | With embodiment 54 | ????0.8∶1 | ????80 | ????PSPc-2-24 |
Embodiment 73～96, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3-1～PSPc-3-24 (liquor)
Above-mentioned glossy ganoderma or/and the fermented liquid of rainbow conk adds stablizer-acetic acid 0.2%～0.5% and/or propionic acid 0.1%～0.5% and/or the citric acid 0.1%～0.5% of 0.1～0.5v%, is made Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3 (liquor).
Concrete preparation condition such as table 4 are listed, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3-1～PSPc-3-24 (liquor), and the effective content of PSPc is not less than 3.5g/L in this additive.
The preparation of table 4, Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-3
| Embodiment | The fermented liquid that uses | The composition of stablizer and addition (V%) | The fodder additives that obtains |
| ???73 | Ganoderma fermentation liquid L-I | Propionic acid 0.1% citric acid 0.2% acetic acid 0.3% | ????PSPc-3-1 |
| ???74 | Rainbow conk fermented liquid Y-I | Propionic acid 0.3% citric acid 0.1% acetic acid 0.5% | ????PSPc-3-2 |
| ???75 | Ganoderma fermentation liquid L-H | Propionic acid 0.5% citric acid 0.1% | ????PSPc-3-3 |
| Acetic acid 0.1% | |||
| ???76 | Rainbow conk fermented liquid Y-II | Propionic acid 0.1% citric acid 0.5% acetic acid 0.2% | ????PSPc-3-4 |
| ???77 | Ganoderma fermentation liquid L-III | Propionic acid 0.1% citric acid 0.5% | ????PSPc-3-5 |
| ???78 | Rainbow conk fermented liquid Y-III | Propionic acid 0.5% citric acid 0.1% | ????PSPc-3-6 |
| ???79 | Ganoderma fermentation liquid L-IV | Acetic acid 0.1% citric acid 0.5% | ????PSPc-3-7 |
| ???80 | Rainbow conk fermented liquid Y-IV | Acetic acid 0.5% citric acid 0.1% | ????PSPc-3-8 |
| ???81 | The fermented liquid LY-1 of glossy ganoderma and rainbow conk | Propionic acid 0.1% acetic acid 0.5% | ????PSPc-3-9 |
| ???82 | Ganoderma fermentation liquid L-V | Propionic acid 0.5% acetic acid 0.1% | ????PSPc-3-10 |
| ???83 | Rainbow conk fermented liquid Y-V | Acetic acid 0.1% | ????PSPc-3-11 |
| ???84 | The fermented liquid LY-II of glossy ganoderma and rainbow conk | Acetic acid 0.3% | ????PSPc-3-12 |
| ???85 | Ganoderma fermentation liquid L-VI | Acetic acid 0.5% | ????PSPc-3-13 |
| ???86 | Rainbow conk fermented liquid Y-VI | Propionic acid 0.1% | ????PSPc-3-14 |
| ???87 | The fermented liquid LY-III of glossy ganoderma and rainbow conk | Propionic acid 0.3% | ????PSPc-3-15 |
| ???88 | The fermented liquid LY-IV of glossy ganoderma and rainbow conk | Propionic acid 0.5% | ????PSPc-3-16 |
| ???89 | Ganoderma fermentation liquid L-VII | Citric acid 0.1% | ????PSPc-3-17 |
| ???90 | The fermented liquid LY-V of glossy ganoderma and rainbow conk | Citric acid 0.3% | ????PSPc-3-18 |
| ???91 | The fermented liquid LY-VI of glossy ganoderma and rainbow conk | Citric acid 0.5% | ????PSPc-3-19 |
| ???92 | The fermented liquid LY-VII of glossy ganoderma and rainbow conk | Propionic acid 0.3% citric acid 0.2% acetic acid 0.3% | ????PSPc-3-20 |
| ???93 | The fermented liquid LY-VIII of glossy ganoderma and rainbow conk | Propionic acid 0.1% citric acid 0.4% acetic acid 0.5% | ????PSPc-3-21 |
| ???94 | The fermented liquid LY-IX of glossy ganoderma and rainbow conk | Citric acid 0.2% acetic acid 0.4% | ????PSPc-3-22 |
| ???95 | The fermented liquid LY-X of glossy ganoderma and rainbow conk | Propionic acid 0.1% acetic acid 0.3% | ????PSPc-3-23 |
| ???96 | The fermented liquid LY-XI of glossy ganoderma and rainbow conk | Citric acid 0.2% acetic acid 0.3% | ????PSPc-3-24 |
Embodiment 97～120, preparation Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4-1～PSPc-4-24 (mycelium pulvis)
1) prepare glossy ganoderma or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid: the method according to embodiment 1～24 prepares glossy ganoderma or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into sterilisable chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, bed thickness 2～3cm, internal cooling to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum size is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and Semen Maydis powder 0.5～5%, sucrose 0.5～2.0%, terra alba 0.5～1.0%;
3) glossy ganoderma is or/and the post-treatment of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4 (mycelium pulvis).
Concrete preparation condition such as table 5 are listed, make Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4-1～PSPc-4-24 (mycelium pulvis), and the effective content of PSPc is not less than 4.0g/kg in this additive.
The preparation of table 5, Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4
| Embodiment | The seed liquor of using | Solid medium is formed (wt%) | Seed liquor inoculum size (V/wt%) | Culture temperature (℃) | Incubation time (greatly) | The fodder additives that obtains |
| ???97 | The Ganderma lucidum seed liquid of embodiment 1 | The dish dregs of rice 20 wheat bran 20 cotton dregs 20 sucrose 0.5 bean cake powder 0.5 terra alba 0.5 wood chip 38 Semen Maydis powder 0.5 | ????5 | ???25 | ??4 | ???PSPc-4-1 |
| ???98 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 8 | The dish dregs of rice 20 wheat bran 20 cotton dregs 20 sucrose 0.5 bean cake powder 0.5 terra alba 0.5 wood chip 38 Semen Maydis powder 0.5 | ????5 | ???25 | ??4 | ???PSPc-4-2 |
| ???99 | The Ganderma lucidum seed liquid of embodiment 2 | Cotton dregs 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 terra albas 0.5 | ????7 | ???27 | ??5 | ???PSPc-4-3 |
| ???100 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 9 | Cotton dregs 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 terra albas 0.5 | ????7 | ???27 | ??5 | ???PSPc-4-4 |
| ???101 | The Ganderma lucidum seed liquid of embodiment 3 | The dish dregs of rice 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 terra albas 0.5 | ????8 | ???28 | ??6 | ???PSPc-4-5 |
| ???102 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 10 | The dish dregs of rice 20 wheat bran 20 bean cake powders 1.0 sucrose 0.5 wood chip 58 terra albas 0.5 | ????8 | ???28 | ??6 | ???PSPc-4-6 |
| ???103 | The Ganderma lucidum seed liquid of embodiment 4 | Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 Semen Maydis powder 3 terra albas 0.5 | ????10 | ???26 | ??7 | ???PSPc-4-7 |
| ???104 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 11 | Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 Semen Maydis powder 3 terra albas 0.5 | ????10 | ??26 | ??7 | ??PSPc-4-8 |
| ???105 | The Ganderma lucidum of embodiment 14 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 Semen Maydis powder 3 terra albas 0.5 | ????9 | ??26 | ??8 | ??PSPc-4-9 |
| ???106 | The Ganderma lucidum seed liquid of embodiment 5 | Bean cake powder 1.0 wheat bran 20 wood chips 75 sucrose 0.5 Semen Maydis powder 3 terra albas 0.5 | ????9 | ??26 | ??8 | ??PSPc-4-10 |
| ???107 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 12 | Bean cake powder 1.0 wheat bran 33 wood chips 60 sucrose 0.5 Semen Maydis powder 5 terra albas 0.5 | ????6 | ??28 | ??9 | ??PSPc-4-11 |
| ???108 | The Ganderma lucidum of embodiment 15 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Bean cake powder 1.0 wheat bran 33 wood chips 60 sucrose 0.5 Semen Maydis powder 5 terra albas 0.5 | ????6 | ??28 | ??9 | ??PSPc-4-12 |
| ???109 | The Ganderma lucidum seed liquid of embodiment 6 | Bean cake powder 2.0 wheat bran 40 wood chips 51 sucrose 1.2 Semen Maydis powders 5 terra albas 0.8 | ????6 | ??28 | ??9 | ??PSPc-4-13 |
| ???110 | The Coriolous Dersicolor (Fr.) Quel fungus seed liquor of embodiment 13 | Bean cake powder 2.0 wheat bran 40 wood chips 51 sucrose 1.2 Semen Maydis powders 5 terra albas 0.8 | ????6 | ??28 | ??9 | ??PSPc-4-14 |
| ???111 | The Ganderma lucidum of embodiment 16 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Bean cake powder 1.5 wheat bran 35 wood chips 60 sucrose 1.5 Semen Maydis powders 2 terra albas 1.0 | ????10 | ??27 | ??10 | ??PSPc-4-15 |
| ???112 | The Ganderma lucidum of embodiment 17 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Bean cake powder 1.5 wheat bran 35 wood chips 57 sucrose 1.5 Semen Maydis powders 5 terra albas 1.0 | ????10 | ??27 | ??10 | ??PSPc-4-16 |
| ???113 | The Ganderma lucidum seed liquid of embodiment 7 | Cotton dregs 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 51 terra albas 0.5 | ????7 | ??27 | ??5 | ??PSPc-4-17 |
| ???114 | The Ganderma lucidum of embodiment 18 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Cotton dregs 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 51 terra albas 0.5 | ????7 | ??27 | ??5 | ??PSPc-4-18 |
| ???115 | The Ganderma lucidum of embodiment 19 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Cotton dregs 40 wheat bran 15 bean cake powders 1.3 sucrose 2.0 wood chips 41 terra albas 0.8 | ????8 | ??29 | ??8 | ??PSPc-4-19 |
| ???116 | The Ganderma lucidum of embodiment 20 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | Cotton dregs 40 wheat bran 15 bean cake powders 1.3 sucrose 2.0 wood chips 41 terra albas 0.8 | ????8 | ??29 | ??8 | ??PSPc-4-20 |
| ???117 | The Ganderma lucidum of embodiment 21 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | The dish dregs of rice 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chip terra albas 1.0 50.5 | ????5 | ??27 | ??7 | ??PSPc-4-21 |
| ???118 | The Ganderma lucidum of embodiment 22 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | The dish dregs of rice 30 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chip terra albas 1.0 50.5 | ????5 | ??27 | ??7 | ??PSPc-4-22 |
| ??119 | The Ganderma lucidum of embodiment 23 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | The dish dregs of rice 40 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 41 terra albas 0.5 | ???8 | ??29 | ??8 | ?PSPc-4-23 |
| ??120 | The Ganderma lucidum of embodiment 24 and Coriolous Dersicolor (Fr.) Quel fungus seed liquor | The dish dregs of rice 40 wheat bran 15 bean cake powders 1.5 sucrose 2.0 wood chips 41 terra albas 0.5 | ???8 | ??29 | ??8 | ?PSPc-4-24 |
Embodiment 121, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are to the influence of laying hen disease resistance, production performance and egg product matter
In the laying hens essential ration as shown in table 6 of filling a prescription, add Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention, different PSPc products all makes an addition to egg approximately in the Preblend (being made up of trace element, VITAMIN etc.), the level that the content of PSPc all reaches in the table 7 to be indicated.
2500 Hainan ash test laying hens (60 ages in week, laying hen later stage) are divided into 10 groups (blank group and interpolation PSPc class group) at random, every group of 5 repetitions, each repeats 50 chickens.4 weeks of trial period.Laying rate, egg size, dead chicken number and yolk colourity, shell thickness, the dense albumen height of each group are listed in table 7.
The feed formulation of table 6, basal diet and trophic level
| Feedstuff raw material | Corn | Dregs of beans | Wheat bran | Stone flour | Secondary calcium phosphate | Salt | Vegetables oil | 1% Preblend * |
| Proportioning % | ??58.72 | ??26.0 | ??2.0 | ??8.4 | ??1.5 | ??0.34 | ???2.0 | ????1.0 |
| Nutritive index | Metabolizable energy (Mcal/kg) | Crude protein (%) | Calcium (%) | Total phosphorus (%) | ||||
| Trophic level | ?????????????2.71 | ??????17.26 | ????????3.50 | ????0.56 | ||||
Production performance between table 7, test group and control group and egg product matter are relatively
| Grouping | ?????1 | ?????2 | ?????3 | ?????4 | ?????5 | ?????6 | ?????7 | ?????8 | ?????9 | ????10 |
| Performance index | Blank 0mg/kg | Add PSPc-1-1 5mg/kg | Add PSPc-1-2 8mg/kg | Add PSPc-1-9 10mg/kg | Add PSPc-2-3 5mg/kg | Add PSPc-2-4 7mg/kg | Add PSPc-2-16 10mg/kg | Add PSPc-4-7 5mg/kg | Add PSPc-4-8 8mg/kg | Add PSPc-4-18 10mg/kg |
| Egg size (g) | ????61.60 | ????63.08 | ????64.19 | ????64.23 | ????63.89 | ????64.12 | ????64.45 | ????63.58 | ????64.17 | ????64.88 |
| Dense albumen height | ????5.55 | ????5.78 | ????5.58 | ????5.98 | ????5.87 | ????5.89 | ????5.90 | ????5.68 | ????5.75 | ????5.98 |
| Yolk colourity | ????5.55 | ????7.46 | ????7.68 | ????8.01 | ????7.32 | ????7.58 | ????8.21 | ????7.16 | ????7.76 | ????8.23 |
| Eggshell strength | ???3.22 | ??3.59 | ??3.67 | ??3.71 | ??3.61 | ??3.69 | ??3.73 | ??3.57 | ??3.65 | ??3.72 |
| Shell thickness | ???364.12 | ??377.04 | ??379.87 | ??380.13 | ??379.45 | ??383.55 | ??386.63 | ??378.44 | ??381.43 | ??386.87 |
| Laying rate | ???82.3 | ??85.5 | ??86.3 | ??86.5 | ??85.3 | ??85.9 | ??86.1 | ??85.6 | ??86.4 | ??86.7 |
| Average egg weight | ???63.5 | ??64.3 | ??64.6 | ??64.8 | ??64.4 | ??64.7 | ??64.8 | ??63.8 | ??64.5 | ??64.9 |
| Feedstuff-egg ratio | ???2.51 | ??2.25 | ??2.23 | ??2.24 | ??2.21 | ??2.26 | ??2.31 | ??2.33 | ??2.28 | ??2.26 |
| Anti-city epidemic disease antibody | ???0.76 | ??1.12 | ??1.23 | ??1.31 | ??1.22 | ??1.19 | ??1.25 | ??1.12 | ??1.32 | ??1.38 |
From the listed result of table 7 as can be seen, feed every index of variant production of the present invention (PSPc-1 class, PSPc-2 class, PSPc-4 class) all significantly better than common mixed feed, particularly the antibody horizontal of yolk colourity and newcastle disease vaccine is significantly higher than control group, illustrate Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention production performance that improves laying hen and egg product matter and improve the antibody horizontal of laying hen and disease resistance aspect tangible good action is all arranged.
Embodiment 122, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are to the influence of fryer in production performance and immunity system growth (disease resistance)
In the broiler chicken in different ages in week, add the PSPc product (seeing Table 9) of different agent, and control group does not add, detect its to immune organ grow, the influence of indexs such as antibody horizontal (disease resistance) and production performance.Test daily ration prescription and the trophic level that reaches see Table 8.Test-results is listed in table 9.
Table 8, broiler chicken daily ration prescription and the trophic level that reaches
| Material name | Proportioning (%) | Nutrient substance | Trophic level | ||
| 0～3 week | 4～6 weeks | 0～3 week | 4～6 weeks | ||
| Corn | ??55.05 | ??62.50 | Metabolizable energy (KCal/kg) | ????3.10 | ????3.10 |
| Dregs of beans | ??32.00 | ??25.35 | Crude protein (%) | ????21.80 | ????20.00 |
| Wheat bran | ??2.30 | ??3.00 | Calcium (%) | ????1.05 | ????1.00 |
| Fish meal (import) | ??3.00 | ??2.50 | Available phosphorus (%) | ????0.51 | ????0.45 |
| Stone flour | ??0.80 | ??0.82 | Methionin (%) | ????1.20 | ????1.05 |
| Vegetables oil | ??3.40 | ??3.12 | Methionine(Met) (%) | ????0.54 | ????0.47 |
| Methionine(Met) | ??0.16 | ??0.07 | Egg+Guang (%) | ????0.90 | ????0.75 |
| Bone meal | ??1.47 | ??1.32 | Salt (%) | ????0.38 | ????0.36 |
| Salt | ??0.25 | ??0.25 | |||
| Compound premix | ??1.00 | ??1.00 | |||
Annotate: per kilogram daily ration cupric 11mg, iodine 0.44mg, iron 110mg, manganese 100mg, selenium 0.25mg, zinc 120mg, cobalt 0.40mg, VA8800IU, VD
33000IU, VE22.5IU, VK
31.8mg, vitamin H 0.2mg, folic acid 1.0mg, nicotinic acid 50mg, pantothenic acid 11mg, riboflavin 6.6mg, VitB1 3.0mg, pyridoxol 4.4mg, VB120.02mg, choline 1300mg.
Table 9, to 6 age in week broiler chicken production performance and the influence of immune organ developmental level
| Grouping | PSPc kind and addition (%) | Body weight (g) | Material anharmonic ratio (F/G) | Thymus gland heavy (g/kg body weight) | Spleen heavy (g/kg body weight) | The fabricius bursa heavy (g/kg body weight) |
| ??1 | Control group (0mg/kg) | ???1302.2 | ????2.29 | ????1.66 | ????1.96 | ????2.18 |
| ??2 | ?PSPc-3-5(10mg/kg) | ???1418.0 | ????2.13 | ????1.99 | ????2.67 | ????2.65 |
| ??3 | ?PSPc-3-6(8mg/kg) | ???1415.7 | ????2.15 | ????1.96 | ????2.52 | ????2.62 |
| ??4 | ?PSPc-3-12(5mg/kg) | ???1408.4 | ????2.19 | ????1.93 | ????2.47 | ????2.60 |
| ??5 | ?PSPc-3-24(9mg/kg) | ???1413.1 | ????2.13 | ????1.98 | ????2.64 | ????2.64 |
| ??6 | ?PSPc-1-10(5mg/kg) | ???1410.2 | ????2.16 | ????1.93 | ????2.43 | ????2.61 |
| ??7 | ?PSPc-1-14(10mg/kg) | ???1419.6 | ????2.12 | ????1.98 | ????2.63 | ????2.63 |
| ??8 | ?PSPc-2-13(5mg/kg) | ???1411.2 | ????2.14 | ????1.97 | ????2.55 | ????2.55 |
| ??9 | ?PSPc-2-15(10mg/kg) | ???1421.3 | ????2.12 | ????2.01 | ????2.64 | ????2.67 |
| ??10 | ?PSPc-4-17(5mg/kg) | ???1415.5 | ????2.15 | ????1.94 | ????2.51 | ????2.62 |
| ??11 | ?PSPc-4-19(10mg/kg) | ???1417.4 | ????2.13 | ????1.99 | ????2.76 | ????2.66 |
Can find out from table 9, the variant production of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention all can significantly improve the speed of growth of broiler chicken, the utilization ratio of feed, and can significantly promote the growth of thymus gland, spleen and the fabricius bursa, improving its relative weight is the immune organ index, but the growth of Polysaccharide-Peptide complex immunologic facilitation fodder additives immune stimulatory organ provided by the invention is described.And immune organ is the vitals and the basic substance of immunity of performance immunocyte and humoral immunization, and increasing of immune organ weight can illustrate that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention can improve the disease resistance of animal.In sum, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention promotes growth of meat chicken and improves disease resistance to be 2 the very important indexs of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention as the microbiotic substitute.
Embodiment 123, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are to the influence of the production performance and the surviving rate of grass carp finishing period
The prescription of grass carp finishing period: test group is a fish meal 15%, soybean meal 25%, cottonseed cake 15%, rice bran 20%, rapeseed cake 10% mixes bran 11.5%, mixed vitamin 1.0%, mixed mineral salt 2.2%, Polysaccharide-Peptide complex immunologic facilitation fodder additives PSPc-4-20 provided by the invention, 10mg/kg; Control group is not for adding Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention.Crude protein content 3 in the prescription is 2.58%.
Test-results shows that the feed coefficient that adds the test group of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention is 1.83, and the feed coefficient of control group is 1.94, has clear improvement; The day weight gain of test group improves 12.3% than control group, surviving rate improves 5.7%, and the reguarity of test group is good, and mortality ratio is low, resistance against diseases is strong, illustrates that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention all has desirable influence to the production performance and the surviving rate of grass carp finishing period.
Embodiment 124, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention influence to milk yield and mastitis incidence in the cow producing milk phase
According to the nutritional need of milk cow, formula feed, prescription sees Table 10, and the nutritive ingredient and the nutrient concentration of daily ration see Table 11.60 3 tire milk cows with about 650 kilograms of body weight, be divided into 2 groups, be test group and control group, every group 30, every component is 3 repetitions, and each repeats 10, raises in the standard milk cattle farm, in the test group daily ration, added the different Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention of 10mg/kg, and control group does not add.Continuous 30 days, detect its milk yield and mastitis incidence every day, be analyzed then, the results are shown in table 12.
Table 10, the phase daily ration of giving milk are formed
Daily ration component type feed title control group proportioning % test group proportioning %
Corn 52 52
Wheat bran 19.8 20
Dregs of beans 24 24
Concentrated feed is formed
Bone meal 3.0 3.0
Stone flour 0.5 0.5
Salt 0.5 0.5
1% milk cow compound premix 1.0 1.0
Add up to 100% 100%
Above-mentioned mixed feed 11kg 11kg
Corn silage 25kg 25kg
Roughage is formed
Sheep's hay 3kg 3kg
Brewer's grains 10kg 10kg
The nutritive ingredient and the nutrient concentration of table 11, daily ration prescription
The milk cow thick egg calcium phosphorus robust fibre of giving milk
Dry-matter
Project amount unit net energy white matter
(kg)
(individual) (MJ) (g) (g) (g) (g)
Nutritive ingredient 20.5 43.31 135.91 3244.50 180.2 125.2 2900.3
Nutrient concentration 2.10 6.6 15.77% 0.88% 0.61% 14.90%
Table 12, milk yield and mastitis incidence
| Grouping and processing | Average milk yield (kg) | Mastitis incidence (%) |
| 1 group (control group, 0mg/kg) | ?????32.86 | ?????5.73 |
| 2 groups (PSPc-1-3,10mg/kg) | ?????34.76 | ?????1.19 |
| 3 groups (PSPc-1-22,10mg/kg) | ?????34.13 | ?????1.31 |
| 4 groups (PSPc-1-23,10mg/kg) | ?????35.03 | ?????1.50 |
| 5 groups (PSPc-2-10,10mg/kg) | ?????34.88 | ?????1.32 |
| 6 groups (PSPc-2-11,10mg/kg) | ?????34.35 | ?????1.33 |
| 7 groups (PSPc-2-12,10mg/kg) | ?????35.12 | ?????1.45 |
| 8 groups (PSPc-3-7,10mg/kg) | ?????35.08 | ?????1.52 |
| 9 groups (PSPc-3-8,10mg/kg) | ?????34.75 | ?????1.22 |
| 10 groups (PSPc-3-21,10mg/kg) | ?????34.82 | ?????1.43 |
| 11 groups (PSPc-4-1,10mg/kg) | ?????35.18 | ?????1.39 |
| 12 groups (PSPc-4-2,10mg/kg) | ?????34.54 | ?????1.32 |
| 13 groups (PSPc-4-23,10mg/kg) | ?????35.23 | ?????1.47 |
From the data of table 12 as can be seen, in dairy cow diet, add dissimilar Polysaccharide-Peptide complex immunologic facilitation feedstuff additive products provided by the invention, can significantly improve the per day milk yield of milk cow, and obviously reduce the mastitis incidence of milk cow.Test shows that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention can significantly improve per day milk yield and the disease resistance (immunizing power) of milk cow.
Embodiment 125, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are to the production performance of weanling pig and the influence of death rate
(1) experimental animal and design: choose body weight and be divided into five processing near 180 healthy of 35 age in days weanling pigs (male and female half and half), each handles 6 repetitions, and each repeats 6 pigs.In 5 weeks of trial period, test design sees Table 13.
Table 13, testing program
Annotate: it is basal diet that the test daily ration adopts the existing daily ration in testing ground, and PSPc makes an addition in the Preblend.
(2) feeding and management and detection index: the fs, (43-49 days) were for raising the phase in advance, observe diarrhoea situation and incidence every day, claim the original weight of every pig in 35 days and divide circle, write down the initial body weight of each multiple, mean body weight and the weight of just expecting, each multiple TBW of weighing in 42 days, mean body weight and residue material amount (8 hours on an empty stomach) are calculated the average daily gain (ADG) in first week, average daily ingestion amount (ADFI) and ADG/ADFI; In each multiple TBW of second week (50-56 days) beginning (49 days) weighing, mean body weight and residue material amount (8 hours on an empty stomach); In like manner, measure each multiple TBW, mean body weight and residue material amount (8 hours on an empty stomach) 56 days, 63 natural gift another names, calculate each all ADG, ADFI and ADG/ADFI.Calculate the average daily gain of fs, average daily ingestion amount, feed efficiency (material anharmonic ratio) and morbidity death condition then.The results are shown in table 14.
Table 14, fs average daily gain, day amount of adopting feed efficiency and death rate
| Group/addition (mg/kg) | Initial mean body weight (Kg) | Mean body weight (Kg) | ???ADG ???(g) | The material anharmonic ratio | Average daily ingestion amount (g) | A dead number of washing in a pan |
| The blank group | ????9.15±0.15 | ????15.92±0.34 | ??271±8 | ???2.23±0.10 | ??603±24 | ????3 |
| The duomycin control group | ????8.89±0.09 | ????17.05±0.55 | ??326±20 | ???1.68±0.08 | ??547±16 | ????1 |
| ????PSPc-1-6 | ????8.91±0.13 | ????16.54±0.42 | ??305±11 | ???1.76±0.08 | ??538±5 | ????1 |
| ????PSPc-1-7 | ????8.99±0.15 | ????17.40±0.61 | ??336±21 * | ???1.68±0.07 | ??566±12 * | ????0 |
| ???PSPc-1-18 | ????9.00±0.12 | ????16.92±0.25 | ??317±10 | ???1.84±0.17 | ??583±36 | ????0 |
Notes: each has 8 pigs in repeating during initial packet, and if any the only dead naughty person of pig, average food consumption was with this pig data rejecting before then basis was eliminated in the test.Blank group of fs whole test phase is dead altogether washes in a pan 3; The microbiotic group is extremely washed in a pan 1 altogether; PSPc-1-6 group (C group) is dead altogether washes in a pan 0; Other group does not have death.
Can find out from table 14, add average daily gain, daily ingestion amount that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention can significantly improve weanling pig, the two all is higher than microbiotic group control group, significant difference (p＜0.05), and death rate significantly is lower than control group (p＜0.01), imitates together with the microbiotic control group.Test shows that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention has tangible promotes growth effect and protection effect, has the antibiotic effect of substituting.
Embodiment 126, Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are to the influence of fryer immunity
For checking Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention in immunoenhancement result on the fryer and the application prospect in livestock industry, test is selected for use and is liked denapon fryer 1 age in days 500 plumages, divide 5 groups, every group of 100 fryer, divide 5 repetitions, each repeats 20 chickens, feed with 5 kinds of different daily rations, be that the A group is the blank group, the B group is the microbiotic group, C organizes feed per ton and adds Polysaccharide-Peptide complex 2.35mg/kg, and D organizes feed per ton and adds Polysaccharide-Peptide complex immunologic facilitation fodder additives 7.05mg/kg provided by the invention, and E organizes feed per ton and adds Polysaccharide-Peptide complex immunologic facilitation fodder additives 11.75mg/kg provided by the invention.7 weeks of trial period.Detecting index is blood lymphocyte increment vigor, the test of bovine serum albumin antibody titers, the results are shown in table 15, table 16 and table 17.
Table 15, to the influence of chicken blood lymphocyte proliferation activity
| Additive and concentration (mg/kg) | Blank (not adding) | Microbiotic contrast (enramycin, 3.2) | ??PSPc-3-10 ????(2.35) | ??PSPc-3-10 ????(7.05) | ??PSPc-3-10 ????(11.75) |
| T cell viability (OD value) | ???2.362 | ???????2.412 | ?????2.320 | ????2.654 | ?????2.872 |
As shown in Table 15, the interpolation of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention in feed can obviously strengthen the T cell viability, but T cell viability and bioactive peptide addition are proportionate, bioactive peptide concentration T cell viability when 11.75mg/kg is the highest, illustrates that the suitableeest addition of bioactive peptide raise immunity is 11.75mg/kg.
Table 16, to the influence of chicken blood bovine serum albumin antibody titers
| Additive and concentration (mg/kg) | Blank (not adding) | Microbiotic (enramycin, 3.2) | ??PSPc-3-10 ????(2.35) | ??PSPc-3-10 ????(7.05) | ??PSPc-3-10 ????(11.75) |
| Antibody titers (OD value) | ???1.112 | ???????1.235 | ????1.725 | ????3.546 | ????6.555 |
As shown in Table 16, the interpolation of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention in feed can obviously strengthen the antibody titers level, and antibody titers level and Polysaccharide-Peptide complex addition are proportionate.
Table 17, meat chicken production performance test-results
| Additive and concentration (mg/kg) | Blank (not adding) | Microbiotic contrast (enramycin, 3.2) | ????PSPc-3-10 ??????(2.35) | ????PSPc-3-10 ??????(7.05) | ?????PSPc-3-10 ??????(11.75) |
| Initial weight (g) | ?????39.1 | ?????39.1 | ?????39.1 | ?????39.1 | ?????39.1 |
| 1-4 week | |||||
| End heavy (g) | ?????935.7 | ?????939.0 | ?????954.4 | ?????977.1 | ?????981.0 |
| Day weight gain (g) | ??32.02±4.13 ABb | ???32.14±2.47 ABab | ???32.72±3.98 Aa | ???35.72±4.24 Aa | ???37.13±5.34 ABab |
| Daily ingestion amount (g) | ??63.89±37.07 | ???64.36±43.52 | ???63.45±32.91 | ???65.02±36.05 | ???67.43±10.13 |
| Feedstuff-meat ratio | ??2.00±0.05 | ???2.00±0.30 | ???1.92±0.07 | ???1.91±0.07 | ???1.94±0.03 |
| Dead number of elements | ????????4 | ?????????4 | ????????1 | ????????2 | ?????????1 |
| 5-7 week | |||||
| End heavy (g) | ????2352 | ????2370 | ????2350 | ????2370 | ????2374 |
| Day weight gain (g) | ????67.44±7.42 | ????68.14±5.76 | ????67.43±4.11 | ????68.14±5.76 | ????69.38±7.16 |
| Daily ingestion amount (g) | ????162.52±18.32 | ????162.76±37.39 | ????159.48±41.76 | ????162.76±37.39 | ????162.88±43.52 |
| Feedstuff-meat ratio | ????2.41±0.06 | ????2.39±0.04 | ????2.35±0.03 | ????2.39±0.04 | ????2.28±0.03 |
| Dead number of elements | ????6 | ????5 | ????4 | ????3 | ????3 |
Annotate: A, B, p between C＜0.01, a, b, p between c＜0.05.
Can find out from table 17, in the broiler chicken feed, add the heavy and efficiency of feed utilization of the day weight gain that can obviously improve broiler chicken, end-body of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention, and the degree that improves and the addition of Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention are proportionate, and can obviously reduce the mortality ratio of broiler chicken.
Comprehensive above example illustrates that Polysaccharide-Peptide complex immunologic facilitation fodder additives provided by the invention is a kind ofly can improve livestock and poultry and aquatic animal production performance, again can strengthening immunity or the immunity enhancement type green feed additive of disease resistance.
Claims (10)
1, a kind of glossy ganoderma or/and the fermented liquid of rainbow conk make by following step:
1) slant culture activation: glossy ganoderma or/and the mycelium of rainbow conk is inoculated on the slant medium of improvement potato dextrose agar, was cultivated 4～10 days down at 25～32 ℃, covered with the inclined-plane until mycelium;
2) preparation shake-flask seed liquid: the mycelial agar slant culture-medium of covering with of obtaining of step 1) is inserted among the liquid nutrient medium A, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～5 days, obtain glossy ganoderma or/and the shake-flask seed liquid of rainbow conk;
3) preparation one grade fermemtation seed liquor: with step 2) shake-flask seed liquid of preparation is pressed 5～20% volume ratio access liquid nutrient medium B, on the shaking table of 80～200 rev/mins of 25～35 ℃, rotating speed, cultivated 2～7 days, obtain glossy ganoderma or/and the one grade fermemtation seed liquor of rainbow conk;
4) submerged fermentation: the one grade fermemtation seed liquor of step 3) preparation is pressed 5～20% volume ratio access liquid nutrient medium C,, obtain glossy ganoderma or/and the fermented liquid of rainbow conk 25～35 ℃ of fermentations 2～7 days.
2, glossy ganoderma as claimed in claim 1 is or/and the fermented liquid of rainbow conk, it is characterized in that, the slant medium of the improvement potato dextrose agar of described step 1) is made by following method: strip or little bulk are cut in potato 200～300 gram peelings, add in the 500ml boiling water and boil after 30 minutes with 4 layers of filtered through gauze, in filtered juice, add 20 gram glucose, 2 gram yeast powders, add water then and be settled to 1000ml, packing, sterilization, standby.
3, glossy ganoderma as claimed in claim 1 is or/and the fermented liquid of rainbow conk is characterized in that described step 2) liquid nutrient medium A be weightmeasurement ratio liquid mixture prepared by following material and water: glucose 4.0%, peptone 0.2%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%, VB
10.002 sterilized 30 minutes for～0.005%, 121 ℃, be cooled to 25 ℃, standby.
4, glossy ganoderma as claimed in claim 1 is or/and the fermented liquid of rainbow conk, it is characterized in that, the liquid nutrient medium B of described step 3) is the weightmeasurement ratio liquid mixture prepared by following material and water: glucose 0.5～1.0%, dregs of beans 2.0～4.0%, corn 5.0～10.0%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%; Or dregs of beans 1.0～2.0%, corn 5.0～10.0%, wheat bran 0.5～1.5%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
4.7H
2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby.
5, glossy ganoderma as claimed in claim 1 is or/and the fermented liquid of rainbow conk, it is characterized in that, the liquid nutrient medium C of described step 4) is in following ratio material water weightmeasurement ratio liquid mixture prepared: dregs of beans 1.0～2.5% is or/and fish meal 0.5～1.0%, corn 5.0～10.0%, wheat bran 0.5～2.0%, feed grade yeast powder 0.5～1.5%, KH
2PO
40.1～0.2%, MgSO
47H
2O 0.05～0.1%, sterilizes 30 minutes for 121 ℃, is cooled to 25 ℃, and is standby.
6, a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives, it is with the described glossy ganoderma of claim 1 or/and the effective content of the Polysaccharide-Peptide complex that makes that the fermented liquid of rainbow conk further concentrates and extracts is not less than the crystal refining product additive of 2.0g/L, and concrete steps are as follows:
With the described glossy ganoderma of claim 1 or/and the centrifugal waste of the fermented liquid of rainbow conk, get supernatant liquor and be evaporated to 40～60% of original volume at 50～80 ℃, and then add the alcohol more than 95% of 1～3 times of volume, staticly settle after the stirring, centrifugation, after oyster white fibery precipitate drying, make Polysaccharide-Peptide complex immunologic facilitation fodder additives.
7, a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives, it is with the described glossy ganoderma of claim 1 or/and the fermented liquid of rainbow conk further concentrates the absorption pulvis that the effective content of the mixed Polysaccharide-Peptide complex that gets of back and carrier is not less than 4.0g/L, and concrete steps are as follows:
With the described glossy ganoderma of claim 1 or/and the fermented liquid of rainbow conk in clarifixator behind the homogeneous, be evaporated to 50% of original volume at 50 ℃, press weight ratio=0.5～0.8 of fermentating liquid volume and mixed carrier: 1 mixed is even, after 60～80 ℃ of following dryings, be crushed to 80 orders, obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives; Described mixed carrier is the mixture by following part by weight preparation: lightweight CaCO
340～60wt%, corn cob meal 20～50wt%, Zein powder 10～30wt%.
8, a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives, it is with the described glossy ganoderma of claim 1 or/and the effective content of the Polysaccharide-Peptide complex that the fermented liquid of rainbow conk interpolation auxiliary material makes is not less than the liquor additive of 3.5g/L, and concrete steps are as follows:
Or/and the fermented liquid of rainbow conk adds the stablizer of 0.1～0.5v%, described stablizer is one or more the mixture that is selected from acetic acid, propionic acid, the citric acid, makes Polysaccharide-Peptide complex immunologic facilitation fodder additives with the described glossy ganoderma of claim 1.
9, a kind of Polysaccharide-Peptide complex immunologic facilitation fodder additives, its effective content for dried mycelial Polysaccharide-Peptide complex is not less than the mycelium pulvis of 4.0g/L, is made by following step:
1) the preparation glossy ganoderma is or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid: be same as the described glossy ganoderma of claim 1 or/and the 1st in the fermented liquid of rainbow conk) step and the 2nd) step;
2) the preparation solid fermentation is cultivated glossy ganoderma or/and prepared from coriolus versicolor mycelium: mycelium solid medium and water are put into stainless steel steamed rice dish after by 1: 1.2 weight ratio mixing, steamed 30 minutes in 100 ℃, move into sterilisable chamber, share afterwards in the stainless steel koji tray of 40cm * 50cm, bed thickness 2～3cm, internal cooling to 25～28 ℃, with the described glossy ganoderma of step 1) or/and the Coriolous Dersicolor (Fr.) Quel fungus shake-flask seed liquid spills equably on the solid fermentation culture base-material inoculates, inoculum size is 5～10%, layering is positioned on the koji tray fermentation carriage then, the control room temperature is 25～29 ℃, relative humidity 80～90%, stirred once in per 2～3 days, cultivated 4～10 days, the glossy ganoderma that promptly gets the solid fermentation cultivation is or/and prepared from coriolus versicolor mycelium; The proportioning of described mycelium solid medium is: the dish dregs of rice 20～40% or/and cotton dregs 20～40% or/and bean cake powder 0.5～2%, wood chip 20～75%, wheat bran 20～40% be or/and Semen Maydis powder 0.5～5%, sucrose 0.5～2.0%, terra alba 0.5～1.0%;
3) glossy ganoderma is or/and the post-treatment of prepared from coriolus versicolor mycelium: with step 2) glossy ganoderma that makes is or/and the prepared from coriolus versicolor mycelium drying, and be crushed to more than 80 orders, promptly obtain Polysaccharide-Peptide complex immunologic facilitation fodder additives.
10, the purposes of a kind of claim 6,7,8 or 9 described Polysaccharide-Peptide complex immunologic facilitation fodder additivess.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387704C (en) * | 2005-04-21 | 2008-05-14 | 中国农业大学 | Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use |
| CN101455274B (en) * | 2008-12-29 | 2011-05-11 | 江南大学 | Method for producing feedstuff containing ganoderma lucidum active ingredient and feedstuff using ganoderma lucidum liquid fermentation liquid |
| CN102550805A (en) * | 2010-12-10 | 2012-07-11 | 吉林农业大学 | Chinese herbal medicine feed additive |
| CN105002095A (en) * | 2014-04-15 | 2015-10-28 | 大自然生机股份有限公司 | Composite ganoderma strain co-culture method and applications thereof |
| CN107779409A (en) * | 2017-11-22 | 2018-03-09 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato scurf bacterium fast breeding culture medium |
| CN113508866A (en) * | 2021-03-26 | 2021-10-19 | 无锡泛宁生物科技有限公司 | Production method of ganoderma lucidum functional feed additive |
| CN114651910A (en) * | 2021-11-24 | 2022-06-24 | 无锡泛宁生物科技有限公司 | Feed additive for promoting growth of broiler chickens and preparation method thereof |
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2004
- 2004-05-21 CN CNB2004100427198A patent/CN1262199C/en not_active Expired - Fee Related
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100387704C (en) * | 2005-04-21 | 2008-05-14 | 中国农业大学 | Lucid ganoderma fungus with high glycopeptide composite yield, its mutagen breeding method and use |
| CN101455274B (en) * | 2008-12-29 | 2011-05-11 | 江南大学 | Method for producing feedstuff containing ganoderma lucidum active ingredient and feedstuff using ganoderma lucidum liquid fermentation liquid |
| CN102550805A (en) * | 2010-12-10 | 2012-07-11 | 吉林农业大学 | Chinese herbal medicine feed additive |
| CN102550805B (en) * | 2010-12-10 | 2014-05-21 | 吉林农业大学 | Chinese herbal medicine feed additive |
| CN105002095A (en) * | 2014-04-15 | 2015-10-28 | 大自然生机股份有限公司 | Composite ganoderma strain co-culture method and applications thereof |
| CN107779409A (en) * | 2017-11-22 | 2018-03-09 | 江苏徐淮地区徐州农业科学研究所(江苏徐州甘薯研究中心) | A kind of sweet potato scurf bacterium fast breeding culture medium |
| CN113508866A (en) * | 2021-03-26 | 2021-10-19 | 无锡泛宁生物科技有限公司 | Production method of ganoderma lucidum functional feed additive |
| CN114651910A (en) * | 2021-11-24 | 2022-06-24 | 无锡泛宁生物科技有限公司 | Feed additive for promoting growth of broiler chickens and preparation method thereof |
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| CN1262199C (en) | 2006-07-05 |
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