CN113508866A - Production method of ganoderma lucidum functional feed additive - Google Patents
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- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 98
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- 239000003674 animal food additive Substances 0.000 title claims abstract description 44
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 31
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- 238000012258 culturing Methods 0.000 claims description 11
- 239000007836 KH2PO4 Substances 0.000 claims description 10
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- 239000002609 medium Substances 0.000 claims description 10
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- 238000000034 method Methods 0.000 claims description 9
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- 238000003756 stirring Methods 0.000 claims description 7
- 239000000843 powder Substances 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 5
- 229920002472 Starch Polymers 0.000 claims description 5
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- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 5
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- 230000001954 sterilising effect Effects 0.000 claims description 5
- 244000068988 Glycine max Species 0.000 claims description 4
- 235000010469 Glycine max Nutrition 0.000 claims description 4
- 238000005273 aeration Methods 0.000 claims description 4
- 241001465754 Metazoa Species 0.000 abstract description 11
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- 150000004676 glycans Chemical class 0.000 abstract description 6
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- RDMQPKIDHAFXKA-JNORPAGFSA-N Ganoderic Acid Am1 Chemical compound C([C@@]12C)C[C@H](O)C(C)(C)[C@@H]1CC(=O)C1=C2C(=O)C[C@]2(C)[C@@H]([C@@H](CC(=O)CC(C)C(O)=O)C)CC(=O)[C@]21C RDMQPKIDHAFXKA-JNORPAGFSA-N 0.000 abstract description 4
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- 229910052732 germanium Inorganic materials 0.000 description 1
- GNPVGFCGXDBREM-UHFFFAOYSA-N germanium atom Chemical compound [Ge] GNPVGFCGXDBREM-UHFFFAOYSA-N 0.000 description 1
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- 239000011573 trace mineral Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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Abstract
The invention provides a production method of a ganoderma lucidum functional feed additive, which comprises the following steps: s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor; s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation broth to obtain homogenized fermentation broth with uniform particle size distribution; and S3, carrying out spray drying on the homogenate fermentation liquor to obtain the ganoderma lucidum functional feed additive. The ganoderma lucidum functional feed additive is prepared by liquid submerged fermentation, homogenization treatment and spray drying, contains rich active substances such as ganoderma lucidum polysaccharide and ganoderic acid, has wide physiological activities such as immunity enhancement, antibacterial property enhancement, virus resistance, tumor resistance, radiation resistance and the like, can obviously improve the quality of animal feed when being used as the feed additive, and enhances the body immunity while improving the quality of meat quality of cultured animals.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a production method of a ganoderma lucidum functional feed additive.
Background
Ganoderma, also known as Rumex, Ganoderma lucidum, Mesona chinensis Benth, Yao grass, caulis et folium Gaultheriae Yunnanensis, LINZHONGLING, JUNLINGZHI, WANNIANZHU, LINGCAO, CHIZHI, DANZHI, QIONGZHEN, etc., is a fungus of Polyporaceae. Ganoderma lucidum is recorded in Ben Cao gang mu compiled by Li Shi Zhen of Ming Dynasty medical scientists. The ganoderma lucidum is rich in polysaccharide, nucleoside, furan, sterol, alkaloid, triterpenes, grease, various amino acids, protein, enzyme, organic germanium, various trace elements and the like; ganoderma polysaccharides has effects in regulating immunity, reducing blood sugar and blood lipid, resisting oxidation, resisting aging and resisting tumor; the triterpenes can purify blood and protect liver function; the Ganoderma preparation has tranquilizing, anticonvulsive, heart tonifying, arrhythmia relieving, blood pressure lowering, cough relieving, and asthma relieving effects; in addition, Ganoderma has anticoagulant, platelet aggregation inhibiting and antiallergic effects. At present, two ways of obtaining ganoderma triterpenes are available, wherein one way is to obtain ganoderma triterpenes from sporocarp and spore powder, and the other way is to obtain ganoderma triterpenes from fermentation liquor or mycelium.
Since the 80 s of the 20 th century, with the continuous 20 years of the livestock breeding industry in China and the high-speed growth of more than 9% per year on average, the problem of shortage of feed resources in livestock production areas will become more and more serious. In recent years, the feed production cost of China is high, the price of raw materials is increased, feed production enterprises are at the margin of loss, the economic benefit of the breeding industry is difficult to guarantee, and the development of the animal husbandry of China is severely restricted; aiming at the current situations of serious shortage of feed resources and high price of raw materials in China, the key technical achievements of conventional energy and protein resource substitution technologies and unconventional feed resource development and utilization in China are integrated, novel feed resources are developed, feed raw material supply is increased, and feed shortage in China is relieved.
Active substances contained in the lucid ganoderma have the characteristics of high efficiency, no toxicity, immunity enhancement, virus resistance, bacteria resistance and the like, so that the lucid ganoderma feed additive is a brand-new and effective green feed additive, and at present, a small amount of reports about preparing the feed additive by the lucid ganoderma are provided, but reports about producing the functional feed additive by using a liquid submerged fermentation mode are not provided.
Therefore, it is required to develop a method for producing a functional feed additive of ganoderma lucidum, thereby improving the quality of animal feed products.
Disclosure of Invention
Aiming at the defects in the prior art, the invention provides a production method of a ganoderma lucidum functional feed additive, which contains rich active substances such as ganoderma lucidum polysaccharide, ganoderic acid and the like and has wide physiological activities of enhancing immunity, antibacterial property, antivirus, antitumor, antiradiation and the like. The technical scheme adopted by the invention is as follows:
a production method of a ganoderma lucidum functional feed additive comprises the following steps: the method comprises the following steps:
s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor;
s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation broth to obtain homogenized fermentation broth with uniform particle size distribution;
and S3, carrying out spray drying on the homogenate fermentation liquor to obtain the ganoderma lucidum functional feed additive.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the step S1 of producing the ganoderma lucidum mycelium liquid submerged fermentation liquid comprises the following steps:
s11, activating the ganoderma lucidum strain: sterilizing a potato glucose agar culture medium at 115-125 ℃ for 20-40 min, then pouring the culture medium into a flat plate to obtain a potato glucose agar culture medium flat plate, and cooling for later use; picking up a ganoderma lucidum block on an ultraclean workbench, placing the ganoderma lucidum block in the center of a potato glucose agar culture medium flat plate, and then putting the potato glucose agar culture medium into a constant-temperature incubator at 20-30 ℃ for culture until ganoderma lucidum mycelia grow full of the flat plate to obtain an activated ganoderma lucidum strain;
s12, preparing a ganoderma lucidum mycelium seed culture solution: 10-30g/L of glucose, 2-7g/L of yeast powder and MgSO4·7H2O 0.5-2.0g/L，KH2PO40.5-2.0g/L, mixing the raw materials to obtain a liquid culture medium, and subpackaging the liquid culture medium in triangular flasks; selecting 4-6 activated Ganoderma strains on a superclean bench, inoculating into a triangular flask, and culturing on a rotary shaking table for 5-14 days to obtain seed culture solution;
s13, preparing mycelium liquid submerged fermentation liquor: adding the fermentation culture medium into a fermentation tank, transferring the seed culture solution into the fermentation tank, stirring, introducing air, and culturing for 3-10 days to obtain Ganoderma mycelia liquid submerged fermentation broth.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the pH of the liquid culture medium in the step S12 is 4.5, and the size of the ganoderma lucidum strain is2-6mm2。
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the temperature of the rotary table in the step S12 is 20-30 ℃, and the rotating speed is 50-200 rpm.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the step S13 fermentation medium comprises soybean flour 10-30g/L, glucose 10-40g/L, soluble starch 5-30g/L, corn flour 5-30g/L, MgSO4·7H2O 0.5-3.0g/L，KH2PO40.5-3.0 g/L; the pH of the fermentation medium was 5.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the inoculation amount of the step S13 is 3-10% (v/v), the fermentation temperature is 20-32 ℃, the stirring speed is 0-200rpm, and the aeration ratio is 0.5-2.0 vvm.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: and S2, controlling the homogenizing temperature to be 40-60 ℃ and the homogenizing pressure to be 10-20 MPa.
Preferably, the production method of the ganoderma lucidum functional feed additive comprises the following steps: the spray drying temperature of the step S3 is 50-60 ℃.
The invention has the advantages that: the ganoderma lucidum functional feed additive is prepared by liquid submerged fermentation, homogenization treatment and spray drying, contains rich active substances such as ganoderma lucidum polysaccharide and ganoderic acid, has wide physiological activities such as immunity enhancement, antibacterial property, antivirus, antitumor and anti-radiation, can obviously improve the quality of animal feed when being used as the feed additive, and can bring great benefits to human health and enhance the body immunity while improving the quality of meat quality of cultured animals.
Detailed Description
The present invention will be further described with reference to the following specific examples.
Example 1
A production method of a ganoderma lucidum functional feed additive comprises the following steps:
s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor;
s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation liquor to obtain homogenized fermentation liquor with uniform particle size distribution, wherein the homogenizing temperature is controlled to be 50 ℃, and the homogenizing pressure is 10 MPa;
and S3, carrying out spray drying on the homogenate fermentation liquor at the temperature of 55 ℃ to obtain the ganoderma lucidum functional feed additive.
The production steps of the step S1 ganoderma lucidum mycelium liquid submerged fermentation liquid specifically comprise:
s11, activating the ganoderma lucidum strain: sterilizing potato glucose agar culture medium at 115 deg.C for 30min, pouring into flat plate to obtain potato glucose agar culture medium flat plate, and cooling; selecting Ganoderma lucidum block of 4mm2 size on an ultraclean workbench, placing in the center of a potato glucose agar culture medium plate, placing the potato glucose agar culture medium in a constant temperature incubator at 26 deg.C, and culturing until Ganoderma lucidum mycelia grows over the plate to obtain activated Ganoderma lucidum strain; the ganoderma lucidum block is ganoderma lucidum, is provided and preserved by Wuxi-Nianning biotechnology limited company, and has the preservation number: CCGMC5.616, published in [ bacteriology newspaper 2020, volume 39, phase 1, 164-;
s12, preparing a ganoderma lucidum mycelium seed culture solution: glucose 20g/L, yeast powder 4g/L, MgSO4·7H2O 1.0g/L，KH2PO4Mixing the above raw materials at a pH of 4.5 and 1.5g/L to obtain a liquid culture medium, and packaging the liquid culture medium in triangular flasks; on the clean bench, 4-6 pieces with size of 4mm are picked2Inoculating the activated Ganoderma strain into a triangular flask, wherein the liquid loading amount is 70% of the volume of the flask, and culturing the triangular flask on a rotary shaking table at 25 deg.C and 120rpm for 8 days to obtain seed culture solution.
S13, preparing mycelium liquid submerged fermentation liquor: the fermentation medium comprises soybean flour 10g/L, glucose 10g/L, soluble starch 10g/L, corn flour 10g/L, MgSO4·7H2O 1.5g/L，KH2PO42.0g/L, pH 5, adding fermentation medium into fermentation tank, liquid loading amount is 70% of fermentation tank volume, and adding seed culture solutionTransferring into a fermentation tank, stirring, introducing air at an inoculation amount of 8% (v/v), a temperature of 27 deg.C, a rotation speed of 50rpm, an aeration ratio of 1.25vvm, and culturing for 6 days to obtain Ganoderma mycelia liquid submerged fermentation broth.
Example 2 feeding animal experiments
Piglet feeding experiments were carried out by using the ganoderma lucidum functional feed additive obtained in example 1, 3 experimental groups and 1 control group were set in total, the control group was fed with a common feed without any additive, and the effect after 60 days of feeding was as shown in table 1
TABLE 1
Example 3
A production method of a ganoderma lucidum functional feed additive comprises the following steps:
s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor;
s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation liquor to obtain homogenized fermentation liquor with uniform particle size distribution, wherein the homogenizing temperature is controlled to be 40 ℃, and the homogenizing pressure is 10 MPa;
and S3, carrying out spray drying on the homogenate fermentation liquor at the temperature of 50 ℃ to obtain the ganoderma lucidum functional feed additive.
The production steps of the step S1 ganoderma lucidum mycelium liquid submerged fermentation liquid specifically comprise:
s11, activating the preserved ganoderma lucidum strain: sterilizing potato glucose agar culture medium at 121 deg.C for 20min, pouring into flat plate to obtain potato glucose agar culture medium flat plate, and cooling; selecting Ganoderma lucidum block of 2mm2 size on an ultraclean workbench, placing in the center of a potato glucose agar culture medium plate, placing the potato glucose agar culture medium in a constant temperature incubator at 20 deg.C, and culturing until Ganoderma lucidum mycelia grows over the plate to obtain activated Ganoderma lucidum strain; the ganoderma lucidum block is ganoderma lucidum, is provided and preserved by Wuxi-Nianning biotechnology limited company, and has the preservation number: CCGMC5.616, published in [ bacteriology newspaper 2020, volume 39, phase 1, 164-;
s12, preparing a ganoderma lucidum mycelium seed culture solution: 10g/L glucose, 2g/L yeast powder and MgSO4·7H2O 0.5g/L，KH2PO4Mixing the above raw materials at a pH of 4.5 and 0.5g/L to obtain liquid culture medium, and packaging the liquid culture medium in triangular flask; on the clean bench, 4-6 pieces with size of 4mm are picked2Inoculating the activated ganoderma strain into a triangular flask, wherein the liquid loading amount is 70% of the volume of the shake flask, and placing the triangular flask on a rotary shaking table for culturing for 5 days at the temperature of 20 ℃ and the rotating speed of 50rpm to obtain a seed culture solution;
s13, preparing mycelium liquid submerged fermentation liquor: the fermentation medium comprises soybean flour 10g/L, glucose 10g/L, soluble starch 5g/L, corn flour 5g/L, MgSO4·7H2O 0.5g/L，KH2PO40.5g/L and pH 5, adding a fermentation medium into a fermentation tank, wherein the liquid loading amount is 70% of the volume of the fermentation tank, transferring a seed culture solution into the fermentation tank, stirring, introducing air, wherein the inoculation amount is 3% (v/v), the temperature is 20 ℃, the rotating speed is 0rpm, the aeration ratio is 0.5vvm, and the culture time is 3 days, so that the ganoderma lucidum mycelium liquid submerged fermentation broth is obtained.
Example 4 feeding animal experiments
Piglet feeding experiments were carried out by using the ganoderma lucidum functional feed additive obtained in example 3, 3 experimental groups and 1 control group were set in total, the control group was fed with a common feed without any additive, and the effect after feeding for 60 days was as shown in table 2.
TABLE 2
Example 5
A production method of a ganoderma lucidum functional feed additive comprises the following steps:
s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor;
s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation liquor to obtain homogenized fermentation liquor with uniform particle size distribution, wherein the homogenizing temperature is controlled to be 60 ℃, and the homogenizing pressure is controlled to be 60 MPa;
and S3, carrying out spray drying on the homogenate fermentation liquor at the temperature of 60 ℃ to obtain the ganoderma lucidum functional feed additive.
The production steps of the step S1 ganoderma lucidum mycelium liquid submerged fermentation liquid specifically comprise:
s11, activating the preserved ganoderma lucidum strain: sterilizing potato glucose agar culture medium at 125 deg.C for 40min, pouring into flat plate to obtain potato glucose agar culture medium flat plate, and cooling; selecting Ganoderma lucidum block of 6mm2 size on an ultraclean workbench, placing in the center of a potato glucose agar culture medium plate, placing the potato glucose agar culture medium in a 30 deg.C constant temperature incubator, and culturing until Ganoderma lucidum mycelia grows over the plate to obtain activated Ganoderma lucidum strain; the ganoderma lucidum block is ganoderma lucidum, is provided and preserved by Wuxi-Nianning biotechnology limited company, and has the preservation number: CCGMC5.616, published in [ bacteriology newspaper 2020, volume 39, phase 1, 164-;
s12, preparing a ganoderma lucidum mycelium seed culture solution: 30g/L glucose, 7g/L yeast powder and MgSO4·7H2O 2g/L，KH2PO4Mixing the raw materials at a pH of 4.5 of 2g/L to obtain a liquid culture medium, and subpackaging the liquid culture medium in triangular flasks; on the clean bench, 4-6 pieces with size of 4mm are picked2Inoculating the activated ganoderma strain into a triangular flask, wherein the liquid loading amount is 70% of the volume of the shake flask, and placing the triangular flask on a rotary shaking table for culturing for 14 days at the temperature of 30 ℃ and the rotation speed of 200rpm to obtain a seed culture solution;
s13, preparing mycelium liquid submerged fermentation liquor: the fermentation medium comprises 30g/L of soybean meal, 40g/L of glucose, 30g/L of soluble starch, 30g/L of corn flour and MgSO4·7H2O 3.0g/L，KH2PO43.0g/L, pH 5, adding the fermentation medium into a fermentation tank, wherein the liquid loading amount is 70% of the volume of the fermentation tank, transferring the seed culture solution into the fermentation tank, stirring and introducing air, wherein the inoculation amount is 10% (v/v), the temperature is 32 ℃, the rotating speed is 200rpm, the air introduction ratio is 2.0vvm, and the culture time is 10 days, so that the ganoderma lucidum mycelium liquid submerged fermentation broth is obtained.
Example 6 experiment on animals fed
Piglet feeding experiments were carried out by using the functional feed additive of ganoderma lucidum obtained in example 5, 3 experimental groups and 1 control group were set in total, and the effect after feeding for 60 days is shown in table 3
TABLE 3
The ganoderma lucidum functional feed additive is prepared by liquid submerged fermentation, homogenization treatment and spray drying, contains rich active substances such as ganoderma lucidum polysaccharide and ganoderic acid, has wide physiological activities such as immunity enhancement, antibacterial property, antivirus, antitumor and anti-radiation, can obviously improve the quality of animal feed when being used as the feed additive, and can bring great benefits to human health and enhance the body immunity while improving the quality of meat quality of cultured animals.
Finally, it should be noted that the above embodiments are only for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to examples, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, which should be covered by the claims of the present invention.
Claims (8)
1. A production method of a ganoderma lucidum functional feed additive is characterized by comprising the following steps: the method comprises the following steps:
s1, producing ganoderma lucidum mycelium liquid submerged fermentation liquor;
s2, homogenizing the ganoderma lucidum mycelium liquid deep fermentation broth to obtain homogenized fermentation broth with uniform particle size distribution;
and S3, carrying out spray drying on the homogenate fermentation liquor to obtain the ganoderma lucidum functional feed additive.
2. The method for producing the ganoderma lucidum functional feed additive according to claim 1, wherein the method comprises the following steps: the step S1 of producing the ganoderma lucidum mycelium liquid submerged fermentation liquid comprises the following steps:
s11, activating the ganoderma lucidum strain: sterilizing a potato glucose agar culture medium at 115-125 ℃ for 20-40 min, then pouring the culture medium into a flat plate to obtain a potato glucose agar culture medium flat plate, and cooling for later use; picking up a ganoderma lucidum block on an ultraclean workbench, placing the ganoderma lucidum block in the center of a potato glucose agar culture medium flat plate, and then putting the potato glucose agar culture medium into a constant-temperature incubator at 20-30 ℃ for culture until ganoderma lucidum mycelia grow full of the flat plate to obtain an activated ganoderma lucidum strain;
s12, preparing a ganoderma lucidum mycelium seed culture solution: 10-30g/L of glucose, 2-7g/L of yeast powder and MgSO4·7H2O 0.5-2.0 g/L，KH2PO40.5-2.0g/L, mixing the raw materials to obtain a liquid culture medium, and subpackaging the liquid culture medium in triangular flasks; selecting 4-6 activated Ganoderma strains on a superclean bench, inoculating into a triangular flask, and culturing on a rotary shaking table for 5-14 days to obtain seed culture solution;
s13, preparing mycelium liquid submerged fermentation liquor: adding the fermentation culture medium into a fermentation tank, transferring the seed culture solution into the fermentation tank, stirring, introducing air, and culturing for 3-10 days to obtain Ganoderma mycelia liquid submerged fermentation broth.
3. The method for producing the ganoderma lucidum functional feed additive according to claim 2, wherein the method comprises the following steps: the pH of the liquid culture medium in the step S12 is =4.5, and the size of the ganoderma lucidum strain is 2-6 mm2。
4. The method for producing the ganoderma lucidum functional feed additive according to claim 2, wherein the method comprises the following steps: the temperature of the rotary table in the step S12 is 20-30 ℃, and the rotating speed is 50-200 rpm.
5. The method for producing the ganoderma lucidum functional feed additive according to claim 2, wherein the method comprises the following steps: the step S13 fermentation medium comprises soybean flour 10-30g/L, glucose 10-40g/L, soluble starch 5-30g/L, corn flour 5-30g/L, MgSO4·7H2O 0.5-3.0 g/L，KH2PO40.5-3.0 g/L; the pH of the fermentation medium = 5.
6. The method for producing the ganoderma lucidum functional feed additive according to claim 2, wherein the method comprises the following steps: the inoculation amount of the step S13 is 3-10% (v/v), the fermentation temperature is 20-32 ℃, the stirring speed is 0-200rpm, and the aeration ratio is 0.5-2.0 vvm.
7. The method for producing the ganoderma lucidum functional feed additive according to claim 1, wherein the method comprises the following steps: and S2, controlling the homogenizing temperature to be 40-60 ℃ and the homogenizing pressure to be 10-20 MPa.
8. The method for producing the ganoderma lucidum functional feed additive according to claim 1, wherein the method comprises the following steps: the spray drying temperature of the step S3 is 50-60 ℃.
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| CN114190485A (en) * | 2021-12-10 | 2022-03-18 | 无锡泛宁生物科技有限公司 | Feed additive for improving pig immunity and preparation method thereof |
| CN114651910A (en) * | 2021-11-24 | 2022-06-24 | 无锡泛宁生物科技有限公司 | Feed additive for promoting growth of broiler chickens and preparation method thereof |
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| CN1600147A (en) * | 2003-09-22 | 2005-03-30 | 中国农业大学 | Immunity strengthened feedstuff additive of glycopeptide composite, preparation method and usage |
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| CN114651910A (en) * | 2021-11-24 | 2022-06-24 | 无锡泛宁生物科技有限公司 | Feed additive for promoting growth of broiler chickens and preparation method thereof |
| CN114176176A (en) * | 2021-11-29 | 2022-03-15 | 无锡泛宁生物科技有限公司 | Feed additive for aquatic animals and preparation method thereof |
| CN114190485A (en) * | 2021-12-10 | 2022-03-18 | 无锡泛宁生物科技有限公司 | Feed additive for improving pig immunity and preparation method thereof |
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