CN104012804B - A kind of compound premix for laying period of layers and application thereof improving intestinal health state - Google Patents
A kind of compound premix for laying period of layers and application thereof improving intestinal health state Download PDFInfo
- Publication number
- CN104012804B CN104012804B CN201410267447.5A CN201410267447A CN104012804B CN 104012804 B CN104012804 B CN 104012804B CN 201410267447 A CN201410267447 A CN 201410267447A CN 104012804 B CN104012804 B CN 104012804B
- Authority
- CN
- China
- Prior art keywords
- parts
- intestinal health
- laying
- compound premix
- health state
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Abstract
The invention discloses a kind of compound premix for laying period of layers and the application thereof that improve intestinal health state, belong to fodder compound technical field.Adopt the ANFs in the complex enzyme formulation removal dregs of beans containing 'beta '-mannase, 1,4 beta-glucanase, acid protease, phytase, the mold toxin sorbent of use yeast cell wall class effectively removes the factor that a large amount of mycotoxin using corn to bring affects intestinal health, stomach invigorating acid is used effectively to adjust the pH value of enteron aisle, the wood chip quality using the present invention to prepare is soft, content of cellulose is high, not postcibal diarrhea, pollution-free, effectively can strengthen the absorption and digestion function of laying hen, bearing function and absorbent function stronger; By the application of above-mentioned various aggregate measures systematic science, guarantee laying cycle of laying hens optimal gut health status, improve immunity of layer chicken and resistance against diseases, play the maximum egg laying performance of laying hen, reduce ight soil water content, significantly improve the maximum economic benefit of the plant using premix.
Description
Technical field
The present invention relates to fodder compound, be specifically related to a kind of compound premix for laying period of layers and the application thereof that improve intestinal health state.
Background technology
Because breeding layer chicken scale is large, use the chicken house of premix many, premix belongs to the minimum feed of industrialization degree compared with complete diet pellet, concentrate feed, and at present, use premix existing problems a lot, subject matter is loose and watery stool, and intestinal health situation is bad.Because dregs of beans use amount is large when using premix to fit in complete diet pellet, in dregs of beans, ANFs content is high, and in dregs of beans, potassium content height is also the factor causing ight soil water content high; Main Antinutritional Factors in dregs of beans is trypsin ihhibitor, and it can reduce pancreas (gruel) proteinase activity, and retarding of growing causes pancreatic regeneration, enlargement; Secondly be soybean agglutinin, cause intestines wall to damage, have inhibitory action to growth of animal, affect digesting and assimilating of nutriment, wherein to the obvious effect of nitrogen metabolism, urinary nitrogen loss obviously increases along with the raising of daily ration agglutinin level; Impact (Li Zhentian, 2003) is had on Intestinal structure and function; Research also finds to gavage the intestinal villi atrophy that soybean protein causes pig, pit cell hyperplasia to piglet, causes Disorder of Digestion and A orption, growth retardation and allergic diarrhea (a watchtower bodyguard man of virtue and ability etc., 1995 simultaneously; ).Use 'beta '-mannase, 1,4 beta-glucanase effectively can reduce the harm of ANFs in dregs of beans.
The daily ration corn use amount using premix to be made into is large, and the corn quality broken kernel content of raiser is high, there is no removal of impurities, so mycotoxin content is just high in corn, destroy the integrality of laying hen intestinal epithelial cell, it is the key factor affecting intestinal health, use the mold toxin sorbent of appropriate yeast cell wall class, beta-mannase, the bioactive ingredients such as beta glucan overcome rapidly the immunosupress that mycotoxin and pathogen are caused while effectively inhaling mould detoxification detoxification, Promote immunity function and resistance against diseases, improve immune response and antibody titer, thus improve intestinal health state, the chicken house daily ration construction materials of 5% premix is single, only have corn, dregs of beans, stone flour, important nutrient in feed---cellulosic nutrient content is low, also be a kind of factor of chicken loose and watery stool, make it excrete because the important physiological action of cellulose nutrition is absorbing toxin, absorption large quantity of moisture, promote intestines peristalsis, being required for poultry intestinal tract normal configuration and function, and being conducive to Escherichia coli synthesis multivitamin in enteron aisle, is the important nutrient ensureing intestinal health.Premix in the market just meets vitamin, trace element, amino acid whose needs, does not consider the needs of chicken intestinal health.The intestinal health of laying hen goes wrong, and brings series of problems accordingly, and loose and watery stool is presentation, and nutrition waste, immunity is poor, premunition is poor, ahead of time eliminate undercarriage, and whole egg production is low, is the greatest problem affecting performance in layers.Therefore, improve laying hen intestinal health extremely important, concrete measure is adjustment gut pH, eliminates ANFs in daily ration, reduces mycotoxin harm, increases crude fibre absorbent function etc.
Summary of the invention
Technical problem solved by the invention is: the defect prepared for existing egg feedstuff compound premix and layer diets, in composite premix feed for laying hen the composite wood chip of science, complex enzyme formulation, mold toxin sorbent, stomach invigorating acid etc. raw material, think that laying hen provides the nutritive additive and cellulose nutrition being rich in and safeguarding intestinal health state, laying hen intestinal health is made to be in optimum state, the rate of digestion of daily ration reaches maximum, play best egg laying performance, reduce ight soil water content, significantly improve the result of use of compound premix.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of compound premix for laying period of layers improving intestinal health state, be made up of the raw material of following parts by weight: carrier 20-40 part, calcium monohydrogen phosphate 15-25 part, zeolite powder 10-20 part, rapeseed dregs 3-15 part, salt 4-8 part, stomach invigorating acid 2-5 part, DL-METHIONINE 2.0-3.5 part, composite trace element 1.0-3.5 part, inhale mould clever 1.5-3 part, complex enzyme formulation 1.0-2.0 part, probiotics 0.8-2 part, Choline Chloride 0.6-2 part, B B-complex 0.4-1.2 part, plain 0.4-1.0 part is imitated with U.S.A, 30 betaine 0.4-0.7 parts, fresh No. 5 pulvis 0.1-0.15 parts,
Described carrier is the cellulose family raw materials such as wood chip, rice chaff, alfalfa meal, not only can be used as the mixed carrier of compound premix, and what is more important can be laying hen and provides nutritional fiber element;
Preferably, described carrier is wood chip, and in general timber, cellulose accounts for 40 ~ 50%, also has the hemicellulose of 10 ~ 30% and the lignin of 20 ~ 30%.Compared with other cellulosic material, wood chip quality is fine and soft soft, content of cellulose is high, not postcibal diarrhea, to laying hen enteron aisle not damaged, be very beneficial for lactic acid bacteria and butyric fermentation, while providing nutritional fiber element for laying hen, more effectively can strengthen the absorption and digestion function of laying hen, bearing function and absorbent function stronger;
The preparation method of described wood chip comprises the steps: to remove foreign material by without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.3-0.4MPa boiling 20-30min, then put microwave dryer in 2000W, 120 DEG C carry out intermittent drying 3min, make it moisture and reach 8-10%, last 2.0 sieves are pulverized, are packed and obtain wood chip;
In described supersonic wave cleaning machine, cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stops 10s, so repeats, cyclic drying.
Described stomach invigorating acid is produced by Shenyang Fengmei Biotechnology Co., Ltd., and registration mark Famebio, consists of phosphatase 11 3 parts by weight, lactic acid 30 parts, fumaric acid 15 parts, citric acid 15 parts, malic acid 2 parts.
Described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, and described carrier is the wood chip of above-mentioned preparation.
The parts by weight of described complex enzyme formulation consist of: phytase 15-20 part, zytase 6-7 part, 'beta '-mannase 5-6 part, 1,4 beta-glucanase 2-3 part, acid protease 3-6 part;
Described mannosan enzyme activity is 1200u/g, and dextranase vigor is 300u/g, and acid protease activity is 3000u/g.
Described probiotics is obtained through mixed culture fermentation, reduced pressure concentration, low-temperature grinding by bacillus subtilis CGMCCNO.7926 and Lactobacillus plantarum CGMCCNO.7928;
Described bacillus subtilis CGMCCNO.7926 and Lactobacillus plantarum CGMCCNO.7928 is the Chinese patent disclosed microorganism fungus kind of application number 201310743042.X;
The preparation method of described probiotics comprises the steps:
Bacillus subtilis CGMCCNO.7926 and Lactobacillus plantarum CGMCCNO.7928 expands cultivation step by step through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot respectively and obtains liquid seeds, then 1-3:3-5 Homogeneous phase mixing by volume, with 10% inoculum concentration access secondary seed tank, cultivate 15-24h for 30-36 DEG C and obtain mixing material seed, mixing speed 200-700r/m, ventilation (V/V) 1:1-3; By mixing material seed with 6% inoculum concentration access fermentation tank, 30-36 DEG C cultivate 10-15h, mixing speed 200-700rpm, ventilation (V/V) 1:1-3, then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, constant temperature culture 15-20h; Continue with 1-2 DEG C/h rate of temperature fall slow cooling to 2-5 DEG C, now, mixing material seed is added access fermentation tank with 4% inoculum concentration, constant temperature culture 20-30h; Finally slowly be warming up to 10-15 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Continue slowly to be warming up to 30-36 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate, zymotic fluid reduced pressure concentration, 20-25 DEG C is crushed to granularity 0.2-0.5mm and namely obtains probiotics;
Described mixing material seed cell concentration is: 7.0x10
8-8.0x10
8individual/ml;
Described zymotic fluid cell concentration is: 7.0x10
10-8.0x10
10individual/ml;
Described probiotics viable bacteria content is: 5.0x10
11-7.0x10
11individual/g.
Described slant medium consists of: beef extract 3-10g, sodium chloride 5-12g, peptone 10-20g, glucose 2-5g, (NH
3)
2sO
43-5g, K
2hPO
46-8g, CaCl
21-3g, Chinese herbal medicine powder 0.5-1.5g, agar 15-20g, distilled water l000mL, pH value 7.0-7.2,121 DEG C of sterilizing 20min;
Described one-level, secondary, three grades of seed culture medium percentage by weights consist of:
Dusty yeast 0.3-0.5%, glucose 1-1.5%, peptone 0.3-0.5%, beef extract 0.5-0.8%, dipotassium hydrogen phosphate 0.8-1.5%, trehalose 1-3%, calcium sulfate 0.1%, magnesium chloride 0.2%, natrium citricum 0.1-0.3%, Chinese herbal medicine powder 0.1-0.3%, insufficient section pure water is supplied, pH value 7.0-7.2,121-123 DEG C of sterilizing 30-40min.
Described one-level, secondary seed tank culture medium percentage by weight consist of:
Maltodextrin 5-15%, dusty yeast 0.4-0.8%, trehalose 1-3%, peptone 0.1-0.5%, corn steep liquor 0.1-0.5%, dipotassium hydrogen phosphate 0.8-1.5%, magnesium sulfate 0.05-0.1%, natrium citricum 0.1-0.5%, Chinese herbal medicine powder 0.5-1.5%, insufficient section pure water is supplied, pH value 7.0-7.2,121-123 DEG C of sterilizing 30-40min.
Described fermentation medium consists of: maltodextrin 50-150g, corn flour 50-60g, beancake powder 15-25g, trehalose 30-40g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1-3g, dipotassium hydrogen phosphate 1-2g, potassium dihydrogen phosphate 1-2g, natrium citricum 1-5g, Chinese herbal medicine powder 20-40g, defoamer 0.1-1g, pure water l000mL, pH value 7.0-7.2,121 DEG C of sterilizing 20min;
The preparation method of described Chinese herbal medicine powder is as follows:
Count by weight, take Radix Astragali 20-30 part, Radix Codonopsis 10-18 part, radix bupleuri 10-15 part, root of large-flowered skullcap 10-15 part, Radix Angelicae Sinensis 8-10 part, cordate houttuynia 8-10 part, motherwort 8-10 part, respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 3-6 times of weight in container, control temperature 70 DEG C ~ 90 DEG C keeps 2 ~ 4h, add the mixture of mixed material 2-3 times of w ethanol and propyl alcohol, the mass ratio of mixing is 1:1-2, Hybrid mode temperature to 60 DEG C ~ 78 DEG C keeps 3 ~ 4h, extract Vacuum Concentration postlyophilization obtains Chinese herbal medicine powder,
Described concentration of alcohol >=75%.
Described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, vitamin B6 1800mg, vitamin B12 1200mg, inositol 5000mg, vitamin E 600mg, vitamine D3 5500mg, Vitamin K3 4000mg; Its surplus is carrier, and described carrier is the wood chip of above-mentioned preparation.
The mould spirit of described suction is that available from Schering Plough (China) Co., Ltd, and the special trading company of Dutch Maas produces, and registration mark Gu doctor, main component is calcium base HSCA, the bioactive ingredients such as beta-mannase, beta glucan.
Described and U.S. effect element is that Nuo Weisi international trade (Shanghai) Co., Ltd. produces, and registration mark is and the plain zero R-HT feed enzyme preparation of U.S. effect, raises No. 002nd, accurate word outside (2011); Main component is beta-mannase.
Described fresh No. 5 pulvis are antioxidant, and produce by building bright industry (Zhuhai) Co., Ltd, registration mark is fresh, main component ethoxyquinoline, butylated hydroxy anisole >=11%.
Described DL-METHIONINE is that EvoinkDegussaGmbH company produces, and registration mark is MetAMINO.
Described Choline Chloride is that Shandong NB Technology Co., Ltd. produces, and registration mark is grace shellfish.
The preparation method of above-mentioned compound premix is:
First rapeseed dregs 2.0 sieves are pulverized for subsequent use, then by carrier and zeolite powder according to mass ratio 2:1 Homogeneous phase mixing, finally namely obtain the compound premix for laying period of layers that can improve intestinal health state according to fill a prescription feeding, Homogeneous phase mixing successively of above-mentioned raw materials.
The using method of above-mentioned compound premix is: mixing composite by above-mentioned compound premix with other day grain raw material is complete feed, and wherein the consumption of compound premix is 5% of complete feed weight.
The application of compound premix of the present invention in layer breeding.
Beneficial effect:
The present invention adopts containing 'beta '-mannase, 1,4 beta-glucanase, acid protease, the complex enzyme formulation of phytase removes the ANFs in dregs of beans, the mold toxin sorbent of use yeast cell wall class effectively removes the factor that a large amount of mycotoxin using corn to bring affects intestinal health, stomach invigorating acid is used effectively to adjust the pH value of enteron aisle, the wood chip quality using the present invention to prepare is soft, content of cellulose is high, not postcibal diarrhea, pollution-free, to laying hen enteron aisle not damaged, be very beneficial for lactic acid bacteria and butyric fermentation, while providing nutritional fiber element for laying hen, more effectively can strengthen the absorption and digestion function of laying hen, bearing function and absorbent function stronger, by the application of above-mentioned various aggregate measures systematic science, guarantee laying cycle of laying hens optimal gut health status, improve immunity of layer chicken and resistance against diseases, play the maximum egg laying performance of laying hen, reduce ight soil water content, significantly improve the maximum economic benefit of the plant using compound premix.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention only limited by claims.To those skilled in the art, under the prerequisite not deviating from essence of the present invention and scope, the various change carry out the material component in these embodiments and consumption or change also belong to protection scope of the present invention.
Composition of raw materials in following examples and source:
Described stomach invigorating acid is produced by Shenyang Fengmei Biotechnology Co., Ltd., and registration mark Famebio, consists of phosphatase 11 3 parts by weight, lactic acid 30 parts, fumaric acid 15 parts, citric acid 15 parts, malic acid 2 parts.
Described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, and described carrier is wood chip prepared by embodiment 1.
The parts by weight of described complex enzyme formulation consist of: phytase 18 parts, zytase 7 parts, 'beta '-mannase 6 parts, 1,4 beta-glucanase 3 parts, acid protease 5 parts;
Described mannosan enzyme activity is 1200u/g, and dextranase vigor is 300u/g, and acid protease activity is 3000u/g.
Described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, vitamin B6 1800mg, vitamin B12 1200mg, inositol 5000mg, vitamin E 600mg, vitamine D3 5500mg, Vitamin K3 4000mg; Its surplus is carrier, and described carrier is wood chip prepared by embodiment 1.
The mould spirit of described suction is that available from Schering Plough (China) Co., Ltd, and the special trading company of Dutch Maas produces, and registration mark Gu doctor, main component is calcium base HSCA, the bioactive ingredients such as beta-mannase, beta glucan.
Described and U.S. effect element is that Nuo Weisi international trade (Shanghai) Co., Ltd. produces, and registration mark is and the plain zero R-HT feed enzyme preparation of U.S. effect, raises No. 002nd, accurate word outside (2011); Main component is beta-mannase.
Described fresh No. 5 pulvis are antioxidant, and produce by building bright industry (Zhuhai) Co., Ltd, registration mark is fresh, main component ethoxyquinoline, butylated hydroxy anisole >=11%.
Described DL-METHIONINE is that EvoinkDegussaGmbH company produces, and registration mark is MetAMINO.
Described Choline Chloride is that Shandong NB Technology Co., Ltd. produces, and registration mark is grace shellfish.
The preparation of embodiment 1 wood chip
The preparation method of wood chip comprises the steps: to remove foreign material by without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.35MPa boiling 25min, then put microwave dryer in 2000W, 120 DEG C carry out intermittent drying 3min, make it moisture and reach 9%, last 2.0 sieves are pulverized, are packed and obtain wood chip;
Described cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stops 10s, so repeats, cyclic drying.
The preparation of embodiment 2 probiotics
The preparation method of described probiotics comprises the steps: that bacillus subtilis CGMCCNO.7926 and Lactobacillus plantarum CGMCCNO.7928 expands cultivation step by step through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot respectively and obtains liquid seeds, then 2:4 Homogeneous phase mixing by volume, with 10% inoculum concentration access secondary seed tank, cultivate 20h for 35 DEG C and obtain mixing material seed, mixing speed 500r/m, ventilation (V/V) 1:2; By mixing material seed with 6% inoculum concentration access fermentation tank, cultivate 12h, mixing speed 400rpm, ventilation (V/V) 1:3 for 33 DEG C, then with 2 DEG C/h rate of temperature fall slow cooling to 12 DEG C, constant temperature culture 15-20h; Continue with 2 DEG C/h rate of temperature fall slow cooling to 4 DEG C, now, mixing material seed is added access fermentation tank with 4% inoculum concentration, constant temperature culture 25h; Finally slowly be warming up to 12 DEG C, constant temperature culture 18h with 2 DEG C/h heating rate; Continue slowly to be warming up to 36 DEG C, constant temperature culture 20h with 2 DEG C/h heating rate, zymotic fluid reduced pressure concentration, 20 DEG C are crushed to granularity 0.4mm and namely obtain probiotics;
Described mixing material seed cell concentration is: 8.0x10
8individual/ml;
Described zymotic fluid cell concentration is: 8.0x10
10individual/ml;
Described probiotics viable bacteria content is: 7.0x10
11individual/g.
Described slant medium consists of: beef extract 7g, sodium chloride 8g, peptone 15g, glucose 3g, (NH
3)
2sO
44g, K
2hPO
47g, CaCl
22g, Chinese herbal medicine powder 1g, agar 18g, distilled water l000mL, pH value 7.2,121 DEG C of sterilizing 20min;
Described one-level, secondary, three grades of seed culture medium percentage by weights consist of:
Dusty yeast 0.4%, glucose 1.2%, peptone 0.4%, beef extract 0.6%, dipotassium hydrogen phosphate 1.2%, trehalose 2%, calcium sulfate 0.1%, magnesium chloride 0.2%, natrium citricum 0.2%, Chinese herbal medicine powder 0.2%, insufficient section pure water is supplied, pH value 7.2,121 DEG C of sterilizing 30min.
Described one-level, secondary seed tank culture medium percentage by weight consist of:
Maltodextrin 10%, dusty yeast 0.6%, trehalose 2%, peptone 0.3%, corn steep liquor 0.3%, dipotassium hydrogen phosphate 1.2%, magnesium sulfate 0.07%, natrium citricum 0.3%, Chinese herbal medicine powder 1%, insufficient section pure water is supplied, pH value 7.2,121 DEG C of sterilizing 30min.
Described fermentation medium consists of: maltodextrin 100g, corn flour 55g, beancake powder 20g, trehalose 35g, dusty yeast 6g, corn steep liquor 3g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, natrium citricum 3g, Chinese herbal medicine powder 30g, defoamer 0.5g, pure water l000mL, pH value 7.2,121 DEG C of sterilizing 20min.
The preparation method of described Chinese herbal medicine powder is as follows:
Count by weight, take the Radix Astragali 25 parts, Radix Codonopsis 15 parts, radix bupleuri 12 parts, the root of large-flowered skullcap 12 parts, Radix Angelicae Sinensis 9 parts, cordate houttuynia 9 parts, motherwort 9 parts, respectively said herbal medicine being crushed to particle diameter is less than 2 millimeters, then Homogeneous phase mixing add the water of 5 times of weight in container, control temperature 80 DEG C keeps 3h, and add the mixture of mixed material 3 times of w ethanol and propyl alcohol, the mass ratio of mixing is 1:1.5, Hybrid mode temperature to 75 DEG C keeps 4h, extract Vacuum Concentration postlyophilization, pulverizes and obtains Chinese herbal medicine powder.
Described concentration of alcohol is 80%.
Embodiment 3
Improve a compound premix for laying period of layers for intestinal health state, be made up of the raw material of following parts by weight: wood chip 30 parts, calcium monohydrogen phosphate 20 parts, zeolite powder 15 parts, rapeseed dregs 11.34 parts, salt 6.5 parts, stomach invigorating acid 3.2 parts, DL-METHIONINE 3.3 parts, composite trace element 3 parts, inhale mould clever 2 parts, complex enzyme formulation 1 part, probiotics 1.4 parts, Choline Chloride 1.2 parts, B B-complex 0.6 part, and U.S.'s effect element 0.5 part, 30 betaines 0.6 part, fresh 0.12 part, No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Namely preparation method: first pulverize for subsequent use by rapeseed dregs 2.0 sieves, then by wood chip and zeolite powder Homogeneous phase mixing, finally obtain the compound premix for laying period of layers that can improve intestinal health state according to fill a prescription feeding, Homogeneous phase mixing successively of above-mentioned raw materials.
Embodiment 4
Improve a compound premix for laying period of layers for intestinal health state, be made up of the raw material of following parts by weight: wood chip 20 parts, calcium monohydrogen phosphate 15 parts, zeolite powder 10 parts, rapeseed dregs 3 parts, salt 4 parts, stomach invigorating acid 2 parts, DL-METHIONINE 2.0 parts, composite trace element 1.0 parts, inhale mould clever 1.5 parts, complex enzyme formulation 1.0 parts, probiotics 0.8 part, Choline Chloride 0.6 part, B B-complex 0.4 part, and U.S.'s effect element 0.4 part, 30 betaines 0.4 part, fresh 0.1 part, No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Preparation method is with embodiment 3.
Embodiment 5
Improve a compound premix for laying period of layers for intestinal health state, be made up of the raw material of following parts by weight: wood chip 40 parts, calcium monohydrogen phosphate 25 parts, zeolite powder 20 parts, rapeseed dregs 15 parts, salt 8 parts, stomach invigorating acid 5 parts, DL-METHIONINE 3.5 parts, composite trace element 3.5 parts, inhale mould clever 3 parts, complex enzyme formulation 2.0 parts, probiotics 2 parts, Choline Chloride 2 parts, B B-complex 1.2 parts, and U.S.'s effect element 1.0 parts, 30 betaines 0.7 part, fresh 0.15 part, No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Preparation method is with embodiment 3.
Embodiment 6 laying hen feeding effect is tested
Test period and raw material:
Tested in March, 2012-mid-June, in Huan Renxian Mu Yuzi town, Liaoning Province Xinhua, chicken house carries out, and control group is common 5% composite premix feed for laying hen, and test group is compound premix prepared by the embodiment of the present invention 3.
Test chicken divides into groups: test adopts single factor experiment design, and experiment material selects red No. 1 commodity egg 1920 in 231 age in days capital, is divided into 2 groups, often organizes 5 repetitions, each repetition 192.
Table 1, of the present invention group of compound premix and common material feed formula composition by weight
| Of the present invention group | Common material group | |
| Dregs of beans | 30 6 --> | |
| Wood chip | 30 | |
| Stone flour | 8 | |
| Zeolite powder | 15 | 7 |
| Rapeseed dregs | 11.34 | 19.44 |
| Calcium monohydrogen phosphate | 20 | 20 |
| Salt | 6.5 | 6.5 |
| DL-METHIONINE | 3.3 | 3.3 |
| 30 betaines | 0.6 | 0.6 |
| Choline Chloride | 1.2 | 1.2 |
| Composite trace element | 3 | 3 |
| B B-complex | 0.6 | 0.6 |
| With U.S.'s effect element | 0.5 |
| Stomach invigorating acid | 3.2 | |
| Fresh No. 5 pulvis | 0.12 | 0.12 |
| Complex enzyme formulation | 1 | |
| Probiotics | 1.4 | |
| Inhale mould spirit | 2 |
Fitting in complete diet pellet recommends proportioning to be: corn 63.2%, dregs of beans 23%, stone flour 8.8%, compound premix 5%.
Result of the test:
Table 2 the present invention is on the impact of laying hen intestinal health and egg laying performance
Note: female different expression significant difference (P < 0.05) of colleague's shoulder marking-up.
In stools scored and ight soil water content, be all better than control group by visible of the present invention group of table 2, illustrate that intestinal health state is better than control group; Ight soil is the first index of reaction intestinal health, and sufficient proof the present invention has laying hen intestinal health situation and significantly improves.
All high than control group in egg size, laying rate, and significant difference.Provable intestinal health state is good, and the digestion and absorption rate of nutrient such as crude protein, energy is high, can show in production performance.Although two feed groups are the same in the nutritive indexs such as crude protein, amino acid, metabolizable energy with large raw material composition, but with U.S. effect is plain, stomach invigorating is sour, enzyme preparation, the bioactive ingredients such as yeast cell wall are to removing mycotoxin, safeguard that the aspects such as intestinal health state adjustment have played the benefit that high-tech brings, absolutely proved science of the present invention and advance.
Claims (4)
1. one kind is improved the compound premix for laying period of layers of intestinal health state, be made up of the raw material of following parts by weight: carrier 20-40 part, calcium monohydrogen phosphate 15-25 part, zeolite powder 10-20 part, rapeseed dregs 3-15 part, salt 4-8 part, stomach invigorating acid 2-5 part, DL-METHIONINE 2.0-3.5 part, composite trace element 1.0-3.5 part, inhale mould clever 1.5-3 part, complex enzyme formulation 1.0-2.0 part, probiotics 0.8-2 part, Choline Chloride 0.6-2 part, B B-complex 0.4-1.2 part, plain 0.4-1.0 part is imitated with U.S.A, 30 betaine 0.4-0.7 parts, fresh No. 5 pulvis 0.1-0.15 parts,
Described carrier is wood chip;
The preparation method of described wood chip comprises the steps: to remove foreign material by without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.3-0.4MPa boiling 20-30min, then put microwave dryer in 2000W, 120 DEG C carry out intermittent drying 3min, make it moisture and reach 8-10%, last 2.0 sieves are pulverized, are packed and obtain wood chip;
In described supersonic wave cleaning machine, cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stops 10s, so repeats, cyclic drying;
The parts by weight of described complex enzyme formulation consist of: phytase 15-20 part, zytase 6-7 part, 'beta '-mannase 5-6 part, 1,4 beta-glucanase 2-3 part, acid protease 3-6 part;
The preparation method of described probiotics comprises the steps: that bacillus subtilis CGMCCNO.7926 and Lactobacillus plantarum CGMCCNO.7928 expands cultivation step by step through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot respectively and obtains liquid seeds, then 1-3:3-5 Homogeneous phase mixing by volume, with 10% inoculum concentration access secondary seed tank, cultivate 15-24h for 30-36 DEG C and obtain mixing material seed, mixing speed 200-700r/m, ventilation (V/V) 1:1-3; By mixing material seed with 6% inoculum concentration access fermentation tank, 30-36 DEG C cultivate 10-15h, mixing speed 200-700rpm, ventilation (V/V) 1:1-3, then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, constant temperature culture 15-20h; Continue with 1-2 DEG C/h rate of temperature fall slow cooling to 2-5 DEG C, now, mixing material seed is added access fermentation tank with 4% inoculum concentration, constant temperature culture 20-30h; Finally slowly be warming up to 10-15 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Continue slowly to be warming up to 30-36 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate, zymotic fluid reduced pressure concentration, 20-25 DEG C is crushed to granularity 0.2-0.5mm and namely obtains probiotics;
Described probiotics viable bacteria content is: 5.0 × 10
11-7.0 × 10
11individual/g.
2. a kind of compound premix for laying period of layers improving intestinal health state as claimed in claim 1, it is characterized in that, described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, and described carrier is wood chip.
3. a kind of compound premix for laying period of layers improving intestinal health state as claimed in claim 1, is characterized in that, described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, vitamin B6 1800mg, vitamin B12 1200mg, inositol 5000mg, vitamin E 600mg, vitamine D3 5500mg, Vitamin K3 4000mg; Its surplus is carrier, and described carrier is wood chip.
4. a kind of compound premix for laying period of layers improving intestinal health state as claimed in claim 1, it is characterized in that, the preparation method of described compound premix is: first pulverize for subsequent use by rapeseed dregs 2.0 sieves, then by carrier and zeolite powder according to mass ratio 2:1 Homogeneous phase mixing, finally namely obtain the compound premix for laying period of layers that can improve intestinal health state according to composition of raw materials successively feeding, Homogeneous phase mixing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267447.5A CN104012804B (en) | 2014-06-16 | 2014-06-16 | A kind of compound premix for laying period of layers and application thereof improving intestinal health state |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201410267447.5A CN104012804B (en) | 2014-06-16 | 2014-06-16 | A kind of compound premix for laying period of layers and application thereof improving intestinal health state |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104012804A CN104012804A (en) | 2014-09-03 |
| CN104012804B true CN104012804B (en) | 2016-04-13 |
Family
ID=51430034
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410267447.5A Active CN104012804B (en) | 2014-06-16 | 2014-06-16 | A kind of compound premix for laying period of layers and application thereof improving intestinal health state |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104012804B (en) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104905077A (en) * | 2015-07-06 | 2015-09-16 | 四川国凤中科生物科技有限公司 | Granulated chicken feed |
| CN105285451A (en) * | 2015-11-04 | 2016-02-03 | 成都心连心农业有限公司 | Compound feed additive for laying hens and preparation method of compound feed additive |
| CN105595008B (en) * | 2016-03-08 | 2021-06-22 | 禾丰食品股份有限公司 | Laying period compound feed for improving healthy chick rate of egg-laying hens and preparation method thereof |
| CN106616233A (en) * | 2016-12-30 | 2017-05-10 | 深圳市前海金卓生物技术有限公司 | Premixed feed for degradating mycotoxin, preparation method thereof and pig daily diet |
| CN106923040A (en) * | 2017-03-06 | 2017-07-07 | 徐州科星科技发展有限公司 | A kind of egg feedstuff for extending laying period and preparation method thereof |
| CN106901060A (en) * | 2017-04-26 | 2017-06-30 | 盐城惠民饲料科技有限公司 | A kind of laying cycle of laying hens green glycolysis mixed feed and preparation method thereof |
| CN107912360B (en) * | 2017-11-09 | 2020-11-24 | 辽宁禾丰牧业股份有限公司 | White feather broiler feeding mode |
| CN107744069A (en) * | 2017-11-23 | 2018-03-02 | 定远县朱湾镇绿农养殖专业合作社 | It is a kind of containing chicken manure and to be not easy smelly rotten feedstuff for live pigs |
| CN114568599A (en) * | 2022-01-05 | 2022-06-03 | 安池(山东)动物营养研究院有限公司 | Premix mate biological function bag for improving intestinal health of laying hens and application thereof |
| CN115005327B (en) * | 2022-06-13 | 2023-08-11 | 河北斐默特生物科技有限公司 | Fermentation type feeding carrier and preparation method thereof |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101218961A (en) * | 2007-01-11 | 2008-07-16 | 河南商都生物技术股份有限公司 | High-efficiency concentration material directly supplied to chicken |
| CN101485397A (en) * | 2009-02-16 | 2009-07-22 | 山东众成饲料科技有限公司 | Premix feed with probiotics for laying hen |
| CN101606634B (en) * | 2009-07-21 | 2012-03-21 | 辽宁众友饲料有限公司 | High-efficacy environment-friendly compound feed for layer hens |
| CN102919548B (en) * | 2012-11-16 | 2013-12-04 | 唐山师范学院 | Ecotype premix additive for fowl and livestock |
| CN103766650B (en) * | 2013-12-27 | 2015-11-18 | 金湖县安农生态农业发展有限公司 | A kind of feed addictive of layer chicken and preparation thereof |
| CN103819239B (en) * | 2014-03-04 | 2016-05-11 | 重庆圣沛农业科技有限公司 | A kind of citrus peel residue biofermentation method |
-
2014
- 2014-06-16 CN CN201410267447.5A patent/CN104012804B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN104012804A (en) | 2014-09-03 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104012804B (en) | A kind of compound premix for laying period of layers and application thereof improving intestinal health state | |
| CN104106731A (en) | Laying hen composite premix | |
| CN103478488B (en) | Pig feed additive capable of effectively improving pork quality and promoting growth | |
| CN104186948A (en) | Total-effect health concentrated feed for pigs | |
| CN105325719A (en) | Growth promoting fodder special for growing and fattening pigs | |
| CN103734549B (en) | Large-grain milk replacer and preparing method thereof | |
| CN105685477A (en) | Preparation method of antibiotic substitute rich in subtilin and effective Chinese herbal medicine ingredients for feeding | |
| CN103598485A (en) | Preparation method and application of Chinese herbal immunopotentiator used for snakeheads | |
| CN109315586A (en) | A kind of preparation method and application of ruminant fermentation of Chinese herbal medicine biological feedstuff | |
| CN105124230A (en) | Taihu sheep feed and production method thereof | |
| CN103667220B (en) | Neutral protease-containing growing pig dedicated enzyme and preparation method thereof | |
| CN105010885A (en) | Conservation concentrated feed with low antigenicity and Chinese herbal medicine | |
| CN108271938B (en) | Functional seaweed feed additive for improving sow constipation | |
| CN103766611B (en) | Neutral protease-containing coarse grain daily ration enzyme and preparation method thereof | |
| CN107319148A (en) | A kind of growth promotion ecological immunity type sika deer special feed and preparation method thereof | |
| CN103844141A (en) | Feed suitable for being used in growing period and fattening period of portunus trituberculatus and use method of feed | |
| CN104041708A (en) | Preparation method for compound premix in egg producing period | |
| CN101194674A (en) | Feed additive for improving pigling intestinal canal function and immunity function, and use method thereof | |
| CN105795387A (en) | Method for preparing fruit enzyme through culture expanding fermentation with fruit juice as culture medium | |
| CN102907585A (en) | Probiotics and vitamin premix and mixture for middle pig | |
| CN106819639A (en) | A kind of large yellow croaker is with new glasswort feed | |
| CN104322948B (en) | A kind of Chinese herbal feed additive and the application in mixed feed thereof | |
| CN105267957B (en) | One boar biological antidiarrheic and its preparation method and application | |
| CN106689817A (en) | Speckled trout puffing mixed feed for resource utilization of salicornia europaea | |
| CN107183400A (en) | A kind of egg feedstuff |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CP03 | Change of name, title or address |
Address after: 110164 Liaoning city of Shenyang province Shenbei New Area Huishan Street No. 169 Patentee after: Hefeng Food Co.,Ltd. Address before: Room 151, building 18, 28-8, Dongling West Road, Shenyang, Liaoning, 110043 Patentee before: Liaoning Hefeng Animal Husbandry Co.,Ltd. |
|
| CP03 | Change of name, title or address |