CN104041708A - Preparation method for compound premix in egg producing period - Google Patents

Preparation method for compound premix in egg producing period Download PDF

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CN104041708A
CN104041708A CN201410268197.7A CN201410268197A CN104041708A CN 104041708 A CN104041708 A CN 104041708A CN 201410268197 A CN201410268197 A CN 201410268197A CN 104041708 A CN104041708 A CN 104041708A
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preparation
compound premix
laying period
parts
premix
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邵素英
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    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention discloses a preparation method for a compound premix in an egg producing period, belonging to the technical field of feed compositions. Anti-nutritional factors are removed from soybean meal through a compound enzyme preparation containing beta-mannase, beta-dextranase, acid proteinase and phytase, and factors, such as mycotoxin, which is caused by use of a large quantity of corns and affect the intestinal health, can be effectively removed through the yeast cell wall type mycotoxin adsorbent; the pH value of the intestinal tract is effectively adjusted by using stomach invigorating acid. Sawdust prepared by the preparation method disclosed by the invention is soft, is high in cellulose content, and is free from pollution; the intestinal tract does not need to be scraped; the digestion and absorption functions of laying hens can be effectively enhanced, and the bearing function and the water absorption function are high. Due to the scientific application of various comprehensive measure systems, the optimal intestinal tract health states of the laying hens in the egg producing period are guaranteed, the immunity and the disease resistance of the laying hens are enhanced, the maximum laying performance of the laying hens is achieved, the moisture content of excrements is reduced, and the maximum economic benefit of a farm using the premix is obviously improved.

Description

A kind of preparation method of compound premix for laying period
Technical field
The present invention relates to fodder compound, be specifically related to a kind of preparation method of compound premix for laying period.
Background technology
Because breeding layer chicken scale is large, use the chicken house of premix many, premix belongs to the feed that industrialization degree is minimum compared with complete diet pellet, concentrate feed, at present, uses premix existing problems a lot, and subject matter is loose and watery stool, and intestinal health situation is bad.Because dregs of beans use amount is large while using premix to fit in complete diet pellet, in dregs of beans, ANFs content is high, and in dregs of beans, potassium content height is also the factor that causes ight soil water content high; Main Antinutritional Factors in dregs of beans is trypsin ihhibitor, and it can reduce pancreas (gruel) proteinase activity, and retarding of growing, causes pancreatic regeneration, enlargement; Secondly be soybean agglutinin, cause the damage of intestines wall, growth of animal is had to inhibitory action, affect digesting and assimilating of nutriment, the wherein obvious effect to nitrogen metabolism, urinary nitrogen loss obviously increases along with the raising of daily ration agglutinin level; Intestinal structure and function is had to impact (Li Zhentian, 2003); Research also finds that gavaging soybean protein to piglet causes the intestinal villi atrophy of pig, and pit cell hyperplasia causes Disorder of Digestion and A orption, growth retardation and allergic diarrhea (a watchtower bodyguard man of virtue and ability etc., 1995 simultaneously; ).Use 'beta '-mannase, 1,4 beta-glucanase can effectively reduce the harm of ANFs in dregs of beans.
The daily ration corn use amount that uses premix to be made into is large, and raiser's corn quality broken kernel content is high, there is no removal of impurities, so mycotoxin content is just high in corn, destroy the integrality of laying hen intestinal epithelial cell, it is the key factor that affects intestinal health, use the mold toxin sorbent of appropriate yeast cell wall class, beta-mannase, the bioactive ingredients such as beta glucan overcome rapidly the immunosupress that mycotoxin and pathogen are caused in effectively inhaling mould detoxification detoxification, Promote immunity function and resistance against diseases, improve immune response and antibody titer, thereby improve intestinal health state, the chicken house daily ration structure raw material of 5% premix is single, only have corn, dregs of beans, stone flour, important nutrient in feed---cellulosic nutrient content is low, also be a kind of factor of chicken loose and watery stool, because the important physiological action of cellulose nutrition is absorbing toxin, it excreted, absorption large quantity of moisture, promote intestines peristalsis, being essential for poultry intestinal tract normal configuration and function, and being conducive to the synthetic multivitamin of Escherichia coli in enteron aisle, is the important nutrient that ensures intestinal health.Premix in the market just meets vitamin, trace element, amino acid whose needs, does not consider the needs of chicken intestinal health.The intestinal health of laying hen goes wrong, and bringing accordingly series of problems, loose and watery stool is presentation, and nutrition waste, immunity is poor, premunition is poor, eliminate ahead of time undercarriage, and whole egg production is low, is the greatest problem that affects performance in layers.Therefore, improve laying hen intestinal health extremely important, concrete measure is for adjusting enteron aisle pH value, eliminate ANFs in daily ration, reduce mycotoxin harm, increasing crude fibre absorbent function etc.
Summary of the invention
Technical problem solved by the invention is: for the defect of existing egg feedstuff compound premix and layer diets preparation, the raw material such as the composite wood chip of science, complex enzyme formulation, mold toxin sorbent, stomach invigorating acid in composite premix feed for laying hen, thinking that laying hen provides is rich in nutritive additive and the cellulose nutrition of safeguarding intestinal health state, make laying hen intestinal health in optimum state, it is maximum that the rate of digestion of daily ration reaches, bring into play best egg laying performance, reduce ight soil water content, significantly improve the result of use of compound premix.
In order to achieve the above object, the present invention is by the following technical solutions:
A kind of preparation method of compound premix for laying period, comprise the steps: that described compound premix is made up of the raw material of following parts by weight: wood chip 30-50 part, calcium monohydrogen phosphate 15-25 part, zeolite powder 15-25 part, rapeseed dregs 3-15 part, salt 4-8 part, stomach invigorating acid 3-6 part, DL-METHIONINE 2.0-3.5 part, composite trace element 1.0-3.5 part, inhale mould clever 1.5-3 part, complex enzyme formulation 1.0-2.0 part, probiotics 1.5-2 part, Choline Chloride 0.6-2 part, B B-complex 0.4-1.2 part, imitate plain 0.4-1.0 part with U.S.A, 30 betaine 0.4-0.7 parts, fresh No. 5 pulvis 0.1-0.15 parts,
First rapeseed dregs is for subsequent use with 2.0 sieves pulverizing, then wood chip is evenly mixed according to mass ratio 2:1 with zeolite powder, finally obtain compound premix for laying period according to composition of raw materials successively feeding, even mixing.
The preparation method of described wood chip comprises the steps: to remove foreign material without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.3-0.4MPa boiling 20-30min, then put microwave dryer and carry out intermittent drying 3min in 2000W, 120 DEG C, make it moisture and reach 8-10%, wood chip is pulverized, packed and obtain to last 2.0 sieves;
In described supersonic wave cleaning machine, cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stop 10s, and so repeat cyclic drying.
Described stomach invigorating acid is produced by Shenyang Fengmei Biotechnology Co., Ltd., registration mark Famebio.
Described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, the wood chip that described carrier is above-mentioned preparation.
The parts by weight of described complex enzyme formulation consist of: phytase 15-20 part, zytase 6-7 part, 'beta '-mannase 5-6 part, 1,4 beta-glucanase 2-3 part, acid protease 3-6 part;
Described mannosan enzyme activity is 1200u/g, and dextranase vigor is 300u/g, and acid protease activity is 3000u/g.
Described probiotics is made through mixed culture fermentation, reduced pressure concentration, low-temperature grinding by bacillus subtilis CGMCC NO.7926 and Lactobacillus plantarum CGMCC NO.7928;
Described bacillus subtilis CGMCC NO.7926 and Lactobacillus plantarum CGMCC NO.7928 are the disclosed microorganism fungus kind of Chinese patent of application number 201310743042.X
The preparation method of described probiotics comprises the steps:
Bacillus subtilis CGMCC NO.7926 and Lactobacillus plantarum CGMCC NO.7928 expand step by step to cultivate through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot respectively and obtain liquid seeds, then 1-3:3-5 evenly mixes by volume, with 10% inoculum concentration access secondary seed tank, cultivate 15-24h for 30-36 DEG C and obtain mixing material seed, mixing speed 200-700r/m, ventilation (V/V) 1:1-3; Mixing material seed, with 6% inoculum concentration access fermentation tank, is cultivated to 10-15h for 30-36 DEG C, mixing speed 200-700rpm, ventilation (V/V) 1:1-3, then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, constant temperature culture 15-20h; Continuation to 2-5 DEG C, now, is appended access fermentation tank, constant temperature culture 20-30h by mixing material seed with 4% inoculum concentration with 1-2 DEG C/h rate of temperature fall slow cooling; Finally slowly be warming up to 10-15 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate; Continue to be slowly warming up to 30-36 DEG C with 1-2 DEG C/h heating rate, constant temperature culture 15-20h, zymotic fluid reduced pressure concentration, 20-25 DEG C is crushed to granularity 0.2-0.5mm and obtains probiotics;
Described mixing material seed cell concentration is: 7.0x10 8-8.0x10 8individual/ml;
Described zymotic fluid cell concentration is: 7.0x10 10-8.0x10 10individual/ml;
Described probiotics viable bacteria content is: 5.0x10 11-7.0x10 11individual/g.
Described slant medium consists of: beef extract 3-10g, sodium chloride 5-12g, peptone 10-20g, glucose 2-5g, (NH 3) 2sO 43-5g, K 2hPO 46-8g, CaCl 21-3g, Chinese herbal medicine powder 0.5-1.5g, agar 15-20g, distilled water l000mL, pH value 7.0-7.2,121 DEG C of sterilizing 20min;
Described one-level, secondary, three grades of seed culture medium percentage by weights consist of:
Dusty yeast 0.3-0.5%, glucose 1-1.5%, peptone 0.3-0.5%, beef extract 0.5-0.8%, dipotassium hydrogen phosphate 0.8-1.5%, trehalose 1-3%, calcium sulfate 0.1%, magnesium chloride 0.2%, natrium citricum 0.1-0.3%, Chinese herbal medicine powder 0.1-0.3%, insufficient section pure water is supplied, pH value 7.0-7.2,121-123 DEG C of sterilizing 30-40min.
Described one-level, secondary seed tank culture medium percentage by weight consist of:
Maltodextrin 5-15%, dusty yeast 0.4-0.8%, trehalose 1-3%, peptone 0.1-0.5%, corn steep liquor 0.1-0.5%, dipotassium hydrogen phosphate 0.8-1.5%, magnesium sulfate 0.05-0.1%, natrium citricum 0.1-0.5%, Chinese herbal medicine powder 0.5-1.5%, insufficient section pure water is supplied, pH value 7.0-7.2,121-123 DEG C of sterilizing 30-40min.
Described fermentation tank culture medium consists of: maltodextrin 50-150g, corn flour 50-60g, beancake powder 15-25g, trehalose 30-40g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1-3g, dipotassium hydrogen phosphate 1-2g, potassium dihydrogen phosphate 1-2g, natrium citricum 1-5g, Chinese herbal medicine powder 20-40g, defoamer 0.1-1g, pure water l000mL, pH value 7.0-7.2,121 DEG C of sterilizing 20min;
The preparation method of described Chinese herbal medicine powder is as follows:
Meter by weight, take Radix Astragali 20-30 part, Radix Codonopsis 10-18 part, radix bupleuri 10-15 part, root of large-flowered skullcap 10-15 part, Radix Angelicae Sinensis 8-10 part, cordate houttuynia 8-10 part, motherwort 8-10 part, respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control temperature 70 C～90 DEG C and keep 2～4h, add the mixture of 2-3 times of weight ethanol of mixed material and propyl alcohol, the mass ratio mixing is 1:1-2, mix and control temperature to 60 DEG C～78 DEG C and keep 3～4h, extract Vacuum Concentration postlyophilization obtains Chinese herbal medicine powder,
Described concentration of alcohol >=75%.
Described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, pyridoxamine 1800mg, cobalamin 1200mg, inositol 5000mg, vitamin E 600mg, neo dohyfral D3 5500mg, prokayvit 4000mg; Its surplus is carrier, the wood chip that described carrier is above-mentioned preparation.
The mould spirit of described suction is that available from Schering Plough (China) Co., Ltd, and the special trading company of Dutch Maas produces, registration mark Gu doctor, and main component is calcium base HSCA, the bioactive ingredients such as beta-mannase, beta glucan.
Described and U.S. effect element is that Nuo Weisi international trade (Shanghai) Co., Ltd. produces, and registration mark is and the plain zero R-HT feed enzyme preparation of U.S. effect that (2011) are outer raises accurate word No. 002; Main component is beta-mannase.
Described fresh No. 5 pulvis are antioxidant, produce by building bright industry (Zhuhai) Co., Ltd, and registration mark is fresh, main component ethoxyquinoline, butylated hydroxy anisole >=11%.
Described DL-METHIONINE is that Evoink Degussa GmbH company produces, and registration mark is Met AMINO.
Described Choline Chloride is that Shandong NB Technology Co., Ltd. produces, and registration mark is grace shellfish.
The using method of the compound premix of preparation is as stated above: it is complete feed that above-mentioned compound premix is mixed composite with other day grain raw material, and wherein the consumption of compound premix is 5% of complete feed weight.
Beneficial effect:
The present invention adopts and contains 'beta '-mannase, 1,4 beta-glucanase, acid protease, the complex enzyme formulation of phytase is removed the ANFs in dregs of beans, use the mold toxin sorbent mycotoxins that effectively a large amount of use of removal corns bring of yeast cell wall class to affect the factor of intestinal health, use stomach invigorating acid effectively to adjust the pH value of enteron aisle, the wood chip quality softness that uses the present invention to prepare, content of cellulose is high, not postcibal diarrhea, pollution-free, to laying hen enteron aisle not damaged, be very beneficial for lactic acid bacteria and butyric fermentation, providing trophism the cellulosic while for laying hen, more can effectively strengthen digestion and the absorption function of laying hen, bearing function and absorbent function are stronger, by the application of above-mentioned various aggregate measures systematic sciences, guarantee laying cycle of laying hens optimal gut health status, improve immunity of layer chicken and resistance against diseases, the maximum egg laying performance of performance laying hen, reduce ight soil water content, significantly improve the maximum economic benefit of the plant that uses compound premix.
Detailed description of the invention
Below by specific embodiment narration the present invention.Unless stated otherwise, in the present invention, technological means used is method known in those skilled in the art.In addition, embodiment is interpreted as illustrative, but not limits the scope of the invention, and the spirit and scope of the invention are only limited by claims.To those skilled in the art, do not deviating under the prerequisite of essence of the present invention and scope various changes that the material component in these embodiments and consumption are carried out or change and also belong to protection scope of the present invention.
Composition of raw materials in following examples and source:
Described stomach invigorating acid is produced by Shenyang Fengmei Biotechnology Co., Ltd., registration mark Famebio.
Described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, and described carrier is wood chip prepared by embodiment 1.
The parts by weight of described complex enzyme formulation consist of: 18 parts of phytases, 7 parts of zytases, 6 parts of 'beta '-mannases, 3 parts of 1,4 beta-glucanases, 5 parts of acid proteases;
Described mannosan enzyme activity is 1200u/g, and dextranase vigor is 300u/g, and acid protease activity is 3000u/g.
Described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, pyridoxamine 1800mg, cobalamin 1200mg, inositol 5000mg, vitamin E 600mg, neo dohyfral D3 5500mg, prokayvit 4000mg; Its surplus is carrier, and described carrier is wood chip prepared by embodiment 1.
The mould spirit of described suction is that available from Schering Plough (China) Co., Ltd, and the special trading company of Dutch Maas produces, registration mark Gu doctor, and main component is calcium base HSCA, the bioactive ingredients such as beta-mannase, beta glucan.
Described and U.S. effect element is that Nuo Weisi international trade (Shanghai) Co., Ltd. produces, and registration mark is and the plain zero R-HT feed enzyme preparation of U.S. effect that (2011) are outer raises accurate word No. 002; Main component is beta-mannase.
Described fresh No. 5 pulvis are antioxidant, produce by building bright industry (Zhuhai) Co., Ltd, and registration mark is fresh, main component ethoxyquinoline, butylated hydroxy anisole >=11%.
Described DL-METHIONINE is that Evoink Degussa GmbH company produces, and registration mark is Met AMINO.
Described Choline Chloride is that Shandong NB Technology Co., Ltd. produces, and registration mark is grace shellfish.
The preparation of embodiment 1 wood chip
The preparation method of wood chip comprises the steps: to remove foreign material without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.35MPa boiling 25min, then put microwave dryer in carrying out intermittent drying 3min in 2000W, 120 DEG C, make it moisture and reach 9%, wood chip is pulverized, packed and obtain to last 2.0 sieves;
Described cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stop 10s, and so repeat cyclic drying.
The preparation of embodiment 2 probioticses
The preparation method of described probiotics comprises the steps: that bacillus subtilis CGMCC NO.7926 and Lactobacillus plantarum CGMCC NO.7928 expand to cultivate through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot respectively step by step and obtains liquid seeds, then 2:4 evenly mixes by volume, with 10% inoculum concentration access secondary seed tank, cultivate 20h for 35 DEG C and obtain mixing material seed, mixing speed 500r/m, ventilation (V/V) 1:2; Mixing material seed, with 6% inoculum concentration access fermentation tank, is cultivated to 12h for 33 DEG C, mixing speed 400rpm, ventilation (V/V) 1:3, then with 2 DEG C/h rate of temperature fall slow cooling to 12 DEG C, constant temperature culture 15-20h; Continue with 2 DEG C/h rate of temperature fall slow cooling to 4 DEG C, now, mixing material seed is appended to access fermentation tank, constant temperature culture 25h with 4% inoculum concentration; Finally slowly be warming up to 12 DEG C with 2 DEG C/h heating rate, constant temperature culture 18h; Continue to be slowly warming up to 36 DEG C with 2 DEG C/h heating rate, constant temperature culture 20h, zymotic fluid reduced pressure concentration, 20 DEG C are crushed to granularity 0.4mm and obtain probiotics;
Described mixing material seed cell concentration is: 8.0x10 8individual/ml;
Described zymotic fluid cell concentration is: 8.0x10 10individual/ml;
Described probiotics viable bacteria content is: 7.0x10 11individual/g.
Described slant medium consists of: beef extract 7g, sodium chloride 8g, peptone 15g, glucose 3g, (NH 3) 2sO 44g, K 2hPO 47g, CaCl 22g, Chinese herbal medicine powder 1g, agar 18g, distilled water l000mL, 7.2,121 DEG C of sterilizing 20min of pH value;
Described one-level, secondary, three grades of seed culture medium percentage by weights consist of:
Dusty yeast 0.4%, glucose 1.2%, peptone 0.4%, beef extract 0.6%, dipotassium hydrogen phosphate 1.2%, trehalose 2%, calcium sulfate 0.1%, magnesium chloride 0.2%, natrium citricum 0.2%, Chinese herbal medicine powder 0.2%, insufficient section pure water is supplied, 7.2,121 DEG C of sterilizing 30min of pH value.
Described one-level, secondary seed tank culture medium percentage by weight consist of:
Maltodextrin 10%, dusty yeast 0.6%, trehalose 2%, peptone 0.3%, corn steep liquor 0.3%, dipotassium hydrogen phosphate 1.2%, magnesium sulfate 0.07%, natrium citricum 0.3%, Chinese herbal medicine powder 1%, insufficient section pure water is supplied, 7.2,121 DEG C of sterilizing 30min of pH value.
Described fermentation tank culture medium consists of: maltodextrin 100g, corn flour 55g, beancake powder 20g, trehalose 35g, dusty yeast 6g, corn steep liquor 3g, ammonium sulfate 2g, dipotassium hydrogen phosphate 2g, potassium dihydrogen phosphate 2g, natrium citricum 3g, Chinese herbal medicine powder 30g, defoamer 0.5g, pure water l000mL, 7.2,121 DEG C of sterilizing 20min of pH value.
The preparation method of described Chinese herbal medicine powder is as follows:
Meter by weight, take 25 parts of the Radixs Astragali, 15 parts of Radix Codonopsis, 12 parts of radix bupleuri, 12 parts of the roots of large-flowered skullcap, 9 parts of Radix Angelicae Sinensis, 9 parts of cordate houttuynias, 9 parts of motherworts, respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 5 times of weight, control 80 DEG C of temperature and keep 3h, add the mixture of 3 times of weight ethanol of mixed material and propyl alcohol, the mass ratio of mixing is 1:1.5, mix and control temperature to 75 DEG C and keep 4h, extract Vacuum Concentration postlyophilization, pulverize and obtain Chinese herbal medicine powder.
Described concentration of alcohol is 80%.
Embodiment 3
A kind of preparation method of compound premix for laying period, comprise the steps: that described compound premix is made up of the raw material of following parts by weight: 40 parts of wood chips, 20 parts of calcium monohydrogen phosphates, 20 parts of zeolite powders, 11.34 parts of rapeseed dregs, 6.5 parts of salt, 5 parts of stomach invigorating acid, 3.3 parts of DL-METHIONINEs, 3 parts of composite trace elements, inhale mould clever 2 parts, 1 part of complex enzyme formulation, 1.8 parts of probioticses, 1.2 parts of Choline Chlorides, 0.6 part of B B-complex, and beautiful 0.5 part of the element of imitating, 30 0.6 part of betaine, 0.12 part, fresh No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Preparation method: first rapeseed dregs is pulverized for subsequent usely by 2.0 sieves, then wood chip is evenly mixed with zeolite powder, finally obtain compound premix for laying period according to fill a prescription successively feeding, even mixing of above-mentioned raw materials.
Embodiment 4
A preparation method for compound premix for laying period, comprises the steps: that described compound premix is made up of the raw material of following parts by weight: 30 parts of wood chips, 15 parts of calcium monohydrogen phosphates, 15 parts of zeolite powders, 3 parts of rapeseed dregs, 4 parts of salt, 3 parts of stomach invigorating acid, 2.0 parts of DL-METHIONINEs, 1.0 parts of composite trace elements, inhale mould clever 1.5 parts, 1.0 parts of complex enzyme formulations, 1.5 parts of probioticses, 0.6 part of Choline Chloride, 0.4 part of B B-complex, and beautiful 0.4 part of the element of imitating, 30 0.4 part of betaine, 0.1 part, fresh No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Preparation method: first rapeseed dregs is pulverized for subsequent usely by 2.0 sieves, then wood chip is evenly mixed with zeolite powder, finally obtain compound premix for laying period according to fill a prescription successively feeding, even mixing of above-mentioned raw materials.
Embodiment 5
A preparation method for compound premix for laying period, comprises the steps: that described compound premix is made up of the raw material of following parts by weight: 50 parts of wood chips, 25 parts of calcium monohydrogen phosphates, 25 parts of zeolite powders, 15 parts of rapeseed dregs, 8 parts of salt, 6 parts of stomach invigorating acid, 3.5 parts of DL-METHIONINEs, 3.5 parts of composite trace elements, inhale mould clever 3 parts, 2.0 parts of complex enzyme formulations, 2 parts of probioticses, 2 parts of Choline Chlorides, 1.2 parts of B B-complexes, and beautiful 1.0 parts of the elements of imitating, 30 0.7 part of betaine, 0.15 part, fresh No. 5 pulvis;
Described wood chip is prepared by embodiment 1;
Described probiotics is prepared by embodiment 2;
Preparation method: first rapeseed dregs is pulverized for subsequent usely by 2.0 sieves, then wood chip is evenly mixed with zeolite powder, finally obtain compound premix for laying period according to fill a prescription successively feeding, even mixing of above-mentioned raw materials.
Embodiment 6 laying hen feeding effect tests
Test period and raw material:
Tested in March, 2012-mid-June, in Huan Renxian Mu Yuzi town, Liaoning Province Xinhua, chicken house carries out, and control group is common 5% composite premix feed for laying hen, and test group is compound premix prepared by the embodiment of the present invention 3.
Test chicken grouping: test adopts single factor experiment design, and experiment material is selected 1920 of red No. 1 commodity eggs in 231 age in days capital, is divided into 2 groups, every group of 5 repetitions, 192 of each repetitions.
Table 1, of the present invention group of compound premix and common material feed formula composition by weight
? Of the present invention group Common material group
Dregs of beans ? 30
Wood chip 40 ?
Stone flour ? 8
Zeolite powder 20 7
Rapeseed dregs 11.34 19.44
Calcium monohydrogen phosphate 20 20
Salt 6.5 6.5
DL-METHIONINE 3.3 3.3
30 betaines 0.6 0.6
Choline Chloride 1.2 1.2
Composite trace element 3 3
B B-complex 0.6 0.6
With U.S.'s effect element 0.5 ?
Stomach invigorating acid 5 ?
Fresh No. 5 pulvis 0.12 0.12
Complex enzyme formulation 1 ?
Probiotics 1.8 ?
Inhale mould spirit 2 ?
Fitting in complete diet pellet recommends proportioning to be: corn 63.2%, dregs of beans 23%, stone flour 8.8%, compound premix 5%.
Result of the test:
The impact of table 2 the present invention on laying hen intestinal health and egg laying performance
Note: the female different significant differences (P < 0.05) that represent of colleague's shoulder marking-up.
Aspect stools scored and ight soil water content, be all better than control group by visible of the present invention group of table 2, illustrating that intestinal health state is better than control group; Ight soil is the first index of reaction intestinal health, fully proves that the present invention has significantly and improves laying hen intestinal health situation.
All high than control group aspect egg size, laying rate, and significant difference.Provable intestinal health state is good, and the digestion and absorption rate of nutrients such as crude protein, energy are high, can show in production performance.Although two feed groups are the same aspect the nutritive indexs such as crude protein, amino acid, metabolizable energy and large raw material composition, but and U.S. effect is plain, stomach invigorating is sour, enzyme preparation, the bioactive ingredients such as yeast cell wall are to removing mycotoxin, safeguard that the aspects such as intestinal health state adjustment have brought into play the benefit that high-tech brings, absolutely proved science of the present invention and advance.

Claims (10)

1. the preparation method of a compound premix for laying period, comprise the steps: that described compound premix is made up of the raw material of following parts by weight: wood chip 30-50 part, calcium monohydrogen phosphate 15-25 part, zeolite powder 15-25 part, rapeseed dregs 3-15 part, salt 4-8 part, stomach invigorating acid 3-6 part, DL-METHIONINE 2.0-3.5 part, composite trace element 1.0-3.5 part, inhale mould clever 1.5-3 part, complex enzyme formulation 1.0-2.0 part, probiotics 1.5-2 part, Choline Chloride 0.6-2 part, B B-complex 0.4-1.2 part, imitate plain 0.4-1.0 part with U.S.A, 30 betaine 0.4-0.7 parts, fresh No. 5 pulvis 0.1-0.15 parts,
First rapeseed dregs is for subsequent use with 2.0 sieves pulverizing, then wood chip is evenly mixed according to mass ratio 2:1 with zeolite powder, finally obtain compound premix for laying period according to composition of raw materials successively feeding, even mixing.
2. the preparation method of a kind of compound premix for laying period as claimed in claim 1, is characterized in that, described composite trace element per kilogram comprises iodine 1000mg, potassium 180mg, iron 1800mg, selenium 80mg, cobalt 200mg, magnesium 145mg, manganese 1400mg, chromium 20mg, zinc 1100mg, copper 800mg; Its surplus is carrier, and described carrier is wood chip.
3. the preparation method of a kind of compound premix for laying period as claimed in claim 1, is characterized in that, described B B-complex per kilogram comprises dehydroretinol 800mg, orotic acid 000mg, Catergen 000mg, riboflavin 1600mg, pantothenic acid 1900mg, pyridoxamine 1800mg, cobalamin 1200mg, inositol 5000mg, vitamin E 600mg, neo dohyfral D3 5500mg, prokayvit 4000mg; Its surplus is carrier, and described carrier is wood chip.
4. the preparation method of a kind of compound premix for laying period as described in as arbitrary in claim 1-3, it is characterized in that, the preparation method of described wood chip comprises the steps: to remove foreign material without mouldy, anosis worm sawmilling end or waste wood, be placed in supersonic wave cleaning machine and clean 10min in 200W, 30KHz, rinse, drain, put into steam still in 0.3-0.4MPa boiling 20-30min, then put microwave dryer and carry out intermittent drying 3min in 2000W, 120 DEG C, make it moisture and reach 8-10%, wood chip is pulverized, packed and obtain to last 2.0 sieves;
In described supersonic wave cleaning machine, cleaning fluid is the sodium bicarbonate solution of mass percent concentration 0.5%;
Described intermittent drying technique is: microwave 10s, stop 10s, and so repeat cyclic drying.
5. the preparation method of a kind of compound premix for laying period as claimed in claim 1, is characterized in that, the parts by weight of described complex enzyme formulation consist of: phytase 15-20 part, zytase 6-7 part, 'beta '-mannase 5-6 part, 1,4 beta-glucanase 2-3 part, acid protease 3-6 part.
6. the preparation method of a kind of compound premix for laying period as claimed in claim 1, it is characterized in that, the preparation method of described probiotics comprises the steps: that bacillus subtilis CGMCC NO.7926 and Lactobacillus plantarum CGMCC NO.7928 are respectively through slant strains activation and one-level, secondary, three grades of liquid seeds and first class seed pot expand step by step to cultivate and obtain liquid seeds, then 1-3:3-5 evenly mixes by volume, with 10% inoculum concentration access secondary seed tank, cultivate 15-24h for 30-36 DEG C and obtain mixing material seed, mixing speed 200-700r/m, ventilation (V/V) 1:1-3, mixing material seed, with 6% inoculum concentration access fermentation tank, is cultivated to 10-15h for 30-36 DEG C, mixing speed 200-700rpm, ventilation (V/V) 1:1-3, then with 1-2 DEG C/h rate of temperature fall slow cooling to 10-15 DEG C, constant temperature culture 15-20h, continuation to 2-5 DEG C, now, is appended access fermentation tank, constant temperature culture 20-30h by mixing material seed with 4% inoculum concentration with 1-2 DEG C/h rate of temperature fall slow cooling, finally slowly be warming up to 10-15 DEG C, constant temperature culture 15-20h with 1-2 DEG C/h heating rate, continue to be slowly warming up to 30-36 DEG C with 1-2 DEG C/h heating rate, constant temperature culture 15-20h, zymotic fluid reduced pressure concentration, 20-25 DEG C is crushed to granularity 0.2-0.5mm and obtains probiotics.
7. the preparation method of a kind of compound premix for laying period as claimed in claim 6, is characterized in that, described one-level, secondary seed tank culture medium percentage by weight consist of: maltodextrin 5-15%, dusty yeast 0.4-0.8%, trehalose 1-3%, peptone 0.1-0.5%, corn steep liquor 0.1-0.5%, dipotassium hydrogen phosphate 0.8-1.5%, magnesium sulfate 0.05-0.1%, natrium citricum 0.1-0.5%, Chinese herbal medicine powder 0.5-1.5%, insufficient section pure water is supplied, pH value 7.0-7.2.
8. the preparation method of a kind of compound premix for laying period as claimed in claim 6, is characterized in that, described fermentation tank culture medium consists of: maltodextrin 50-150g, corn flour 50-60g, beancake powder 15-25g, trehalose 30-40g, dusty yeast 4-8g, corn steep liquor 1-5g, ammonium sulfate 1-3g, dipotassium hydrogen phosphate 1-2g, potassium dihydrogen phosphate 1-2g, natrium citricum 1-5g, Chinese herbal medicine powder 20-40g, defoamer 0.1-1g, pure water l000mL, pH value 7.0-7.2.
9. the preparation method of a kind of compound premix for laying period as described in as arbitrary in claim 7-8, it is characterized in that, the preparation method of described Chinese herbal medicine powder is as follows: meter by weight, take Radix Astragali 20-30 part, Radix Codonopsis 10-18 part, radix bupleuri 10-15 part, root of large-flowered skullcap 10-15 part, Radix Angelicae Sinensis 8-10 part, cordate houttuynia 8-10 part, motherwort 8-10 part, respectively said herbal medicine being crushed to particle diameter is below 2 millimeters, then in container, evenly mix and add the water of 3-6 times of weight, control temperature 70 C～90 DEG C and keep 2～4h, add the mixture of 2-3 times of weight ethanol of mixed material and propyl alcohol, the mass ratio mixing is 1:1-2, mix and control temperature to 60 DEG C～78 DEG C and keep 3～4h, extract Vacuum Concentration postlyophilization obtains Chinese herbal medicine powder,
Described concentration of alcohol >=75%.
10. the preparation method of a kind of compound premix for laying period as claimed in claim 6, is characterized in that, described probiotics viable bacteria content is: 5.0x10 11-7.0x10 11individual/g.
CN201410268197.7A 2014-06-16 2014-06-16 Preparation method for compound premix in egg producing period Pending CN104041708A (en)

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