CN113973530B - Method for promoting graft scion shelling of watermelon grafted seedlings - Google Patents
Method for promoting graft scion shelling of watermelon grafted seedlings Download PDFInfo
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- CN113973530B CN113973530B CN202111362143.3A CN202111362143A CN113973530B CN 113973530 B CN113973530 B CN 113973530B CN 202111362143 A CN202111362143 A CN 202111362143A CN 113973530 B CN113973530 B CN 113973530B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/05—Fruit crops, e.g. strawberries, tomatoes or cucumbers
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- Developmental Biology & Embryology (AREA)
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Abstract
The invention discloses a method for promoting the shelling of watermelon grafted seedling scions, which comprises the following steps: step 1: soaking watermelon seeds in warm soup at 45-55 ℃ for 20-40 minutes, and step 2: after seed soaking, placing the seeds in a gibberellin solution with the concentration of 600ppm to 1000ppm for soaking for 3 to 5 hours, then cleaning and draining, and the step 3: and (4) accelerating germination of the drained watermelon seeds, and step 4: and (4) sowing and covering the germinated watermelon seeds, and continuing to accelerate germination for 3-5 days until the height of the seedlings exceeds 8cm, thus obtaining the scion seedlings for watermelon grafting. According to the method for promoting the scion shelling of the watermelon grafted seedling, provided by the invention, the natural shelling rate of the scion can be effectively improved through seed soaking and germination acceleration of gibberellin and river sand covering, the 'capped' unearthing of the scion seed is greatly reduced, the labor for manually removing the scion seed shell is saved, the damage rate of the cotyledon of the scion is reduced, the scion seedling forming time is saved by 1-2 days compared with that of a common method, and a good foundation is laid for the development of the watermelon grafted seedling work.
Description
Technical Field
The invention relates to a method for promoting graft scion shelling of watermelon seedlings, and belongs to the technical field of watermelon cultivation.
Background
The watermelon is one of fruits which people like to eat, the cultivation area of the watermelon in China is 189.72 ten thousand hectares, and the yield of the watermelon is 7939.58 ten thousand tons. The watermelon is planted specially, if the watermelon is planted on the same land for two years, the disease resistance of plants is reduced, a great amount of blight occurs to cause serious watermelon death, the yield is reduced if the watermelon is light, and the watermelon cannot be harvested if the watermelon is heavy, so that the watermelon is a great obstacle which seriously affects the watermelon industry.
In order to solve the problem of continuous cropping obstacle of watermelons, people adopt a grafting seedling raising method to graft seedlings of watermelons onto rootstock plants such as cucurbits or pumpkins, wherein only cotyledons are reserved, and the method can effectively prevent the continuous cropping problems such as blight and the like, prevent the problems of yield reduction and even complete harvest and the like, and enable the watermelons to be continuously cropped, so that the method is widely adopted.
However, in the process of grafting and seedling raising of watermelon, scion (watermelon) seeds often have shells and come out of the soil, the shells on the scion cotyledons need to be manually removed, the seedling raising cost is greatly increased, and the scion leaves are easy to damage and are susceptible to diseases, so that an effective scion shelling technology is urgently needed to reduce the number of 'capped' seeds coming out of the soil, improve the uniformity and consistency of the seedling coming out of the soil and facilitate the development of the watermelon grafting work.
Gibberellin is used as an important plant hormone, plays an important role in regulation and control of seed germination, leaf extension, stem and root elongation, flower and fruit development and the like, and is widely used in daily management of crops. At present, gibberellin is reported to promote plant seed germination, mainly in that the gibberellin can improve the germination rate and the germination vigor of seeds, but the research on the shelling influence of plant seeds with thicker seed coats is not reported yet.
Disclosure of Invention
In order to solve the technical problems, the invention greatly improves the natural shelling rate of the watermelon grafted seedling scion, reduces the labor for seed shelling, avoids the damage of seed leaves of seedlings caused by manual seed shelling and reduces the possibility of disease infection of the scion by soaking gibberellin and changing a covering on the basis of not changing the original watermelon scion cultivation method. In order to achieve the purpose of automatically shelling watermelon scions, the invention discloses a method for promoting shelling of watermelon grafted seedlings scions, which comprises the following specific technical scheme:
step 1: soaking watermelon seeds in warm soup at 45-55 deg.C for 20-40 min,
step 2: soaking in 600-1000 ppm gibberellin solution for 3-5 hr, washing, filtering,
and 3, step 3: the filtered watermelon seeds are subjected to pregermination,
and 4, step 4: and (4) sowing and covering the germinated watermelon seeds, and continuing to accelerate germination for 3-5 days until the height of the seedlings exceeds 8cm, thus obtaining the scion seedlings for watermelon grafting.
Further, in the step 1, the broth temperature is 55 ℃, and the seed soaking time is 30 minutes.
Furthermore, the gibberellin solution in step 2 has the best concentration of 800ppm, and the soaking time is specifically 4 hours.
Furthermore, in the germination accelerating process in the step 3, the watermelon seeds are wrapped with air-permeable and moisture-retaining wrappings in the whole process.
Further, the seeding and covering specific operation process in the step 4 comprises the following steps: sowing the germinated watermelon seeds in a flat-bottom plate, covering the seeds with river sand, wherein the adopted matrix is peat and perlite mixed compound matrix.
Furthermore, the river sand coverage thickness is 0.3-1cm, and the used matrix is specifically compounded by peat and perlite according to the volume ratio of 3: 1.
Further, the river sand coverage thickness is specifically 0.5 cm.
Further, the germination temperature in step 3 and step 4 is 30 ℃.
Further, in the step 4, the germination is continued for 4 days until the height of the seedling is 10cm, and the scion seedling can be used for watermelon grafting.
Further, the breathable and moisture-preserving wrap is specifically a sterile towel or gauze.
Has the advantages that: according to the method for promoting the scion shelling of the watermelon grafted seedling, provided by the invention, the natural shelling rate of the scion can be effectively improved through seed soaking and germination acceleration of gibberellin and river sand covering, the 'capped' unearthing of the scion seed is greatly reduced, the labor for manually removing the scion seed shell is saved, the damage rate of the cotyledon of the scion is reduced, the scion seedling forming time is saved by 1-2 days compared with that of a common method, and a good foundation is laid for the development of the watermelon grafted seedling work.
Detailed Description
The present invention is further illustrated by the following examples, which are intended to be purely exemplary and are not intended to limit the scope of the invention, as various equivalent modifications of the invention will occur to those skilled in the art upon reading the present disclosure and fall within the scope of the appended claims.
The method for promoting the graft scion shelling of the watermelon grafted seedlings comprises the following steps:
step 1: soaking watermelon seeds in warm soup at 45-55 deg.C for 20-40 min,
and 2, step: soaking in 600-1000 ppm gibberellin solution for 3-5 hr, washing, filtering,
and step 3: the filtered watermelon seeds are subjected to pregermination,
and 4, step 4: and (4) sowing and covering the germinated watermelon seeds, and continuing to accelerate germination for 3-5 days until the height of the seedlings exceeds 8cm, thus obtaining the scion seedlings for watermelon grafting.
In the step 1, the optimal temperature of the broth is 55 ℃, and the seed soaking time is 30 minutes. In the step 2, the gibberellin solution has the best concentration of 800ppm, and the soaking time is the best time of 4 hours. And 3, in the germination accelerating process, the watermelon seeds are wrapped with a breathable and moisture-preserving wrapper, specifically an aseptic towel or gauze. Step 4, the specific operation process of sowing and covering is as follows: sowing the germinated watermelon seeds in a flat-bottom plate, covering with river sand, wherein the thickness of the river sand coverage is 0.3-1cm, preferably 0.5cm, and the adopted matrix is peat and perlite mixed compound matrix which is specifically prepared by compounding peat and perlite according to the volume ratio of 3: 1. The germination accelerating temperature in the step 3 and the step 4 is preferably 30 ℃. And 4, continuing germination for 4 days until the height of the seedling is 10cm, and obtaining the scion seedling for watermelon grafting.
Example 1:
step 1: soaking 100 watermelon seeds in warm water at 55 ℃ for 30 minutes,
step 2: then placing the mixture into a gibberellin solution with the concentration of 600ppm to be soaked for 3 hours, cleaning and filtering the mixture to be dry,
and step 3: wrapping with towel with good air permeability and moisture retention, placing into a germination accelerating box for accelerating germination until seeds germinate,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom plate, covering by river sand with the thickness of 0.3cm, and compounding peat and perlite according to a volume ratio of (3:1) to form a matrix. After the flat-bottom tray is sowed and covered, pregermination is carried out in a germination accelerating room, and scion seedlings for watermelon grafting can be obtained after the height of the scion reaches 10 cm.
Example 2
Step 1: soaking 100 watermelon seeds in warm soup at 55 ℃ for 30 minutes,
step 2: then placing the mixture into 800ppm gibberellin solution to be soaked for 4 hours, cleaning and filtering the mixture to be dry,
and step 3: wrapping with towel with good air permeability and moisture retention, placing into a germination accelerating box for accelerating germination until seeds germinate,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom plate, covering with river sand with the thickness of 0.5cm, and compounding peat and perlite according to the volume ratio of (3:1) to obtain the matrix. After the flat-bottom tray is sowed and covered, pregermination is carried out in a germination accelerating room, and scion seedlings for watermelon grafting can be obtained after the height of the scion reaches 10 cm.
Example 3
Step 1: soaking 100 watermelon seeds in warm soup at 55 ℃ for 30 minutes,
step 2: then placing the mixture into a gibberellin solution with the concentration of 1000ppm for soaking for 5 hours, cleaning and filtering the mixture,
and step 3: wrapping with towel with good air permeability and moisture retention, placing into a germination accelerating box for accelerating germination until seeds germinate,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom plate, covering with river sand with the thickness of 0.8cm, and compounding peat and perlite according to the volume ratio of (3:1) to obtain the matrix. After the flat-bottom tray is sowed and covered, pregermination is carried out in a germination accelerating room, and scion seedlings for watermelon grafting can be obtained after the height of the scion reaches 10 cm.
Example 4
Step 1: soaking 100 watermelon seeds in warm soup at 55 ℃ for 30 minutes,
step 2: then placing the mixture into 800ppm gibberellin solution to be soaked for 4 hours, cleaning and filtering the mixture to be dry,
and step 3: wrapping with towel with good air permeability and moisture retention, placing into a germination accelerating box for accelerating germination until seeds germinate,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom plate, covering with river sand with the thickness of 0.8cm, and compounding peat and perlite according to the volume ratio of (3:1) to obtain the matrix. After the flat-bottom tray is sowed and covered, pregermination is carried out in a germination accelerating room, and scion seedlings for watermelon grafting can be obtained after the height of the scion reaches 10 cm.
Comparative example 1
Step 1: soaking 100 watermelon seeds in warm soup at 55 ℃ for 30 minutes,
step 2: then soaking for 4 hours, cleaning, filtering to dry,
and step 3: wrapping with towel with good air permeability and moisture retention, placing into a germination accelerating box for accelerating germination until seeds germinate,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom tray, covering by seedling raising medium with the thickness of 1cm, wherein the medium is formed by compounding peat and perlite according to the volume ratio of (3: 1). After the flat-bottom tray is sowed and covered, pregermination is carried out in a germination accelerating room, and scion seedlings for watermelon grafting can be obtained after the height of the scion reaches 10 cm.
Statistics of results
The germination rates and emergence rates of the seeds in examples 1 to 4 and comparative example 1 were counted, and the shelling rates of the scions were continuously counted after the scions were established. The data are shown in table 1.
TABLE 1 germination rates, spontaneous shelling rates and seedling time of scions of watermelon in examples 1-4 and comparative examples
Treatment group | Percentage of germination (%) | Percent ratio of natural shelling (%) | Time to seedling (d) |
Example 1 | 86% | 43% | 3 |
Example 2 | 91% | 48% | 3 |
Example 3 | 87% | 45% | 3 |
Example 4 | 91% | 47% | 4 |
Comparative example 1 | 81% | 28% | 5 |
Conclusion
As can be seen from Table 1, the natural shelling rate and the seedling days of the watermelon scions in examples 1 to 4 are both superior to those of comparative example 1, the germination rate and the natural shelling rate of the scions can be improved by soaking the watermelon seeds with gibberellin and adding a proper covering, the natural shelling rate is improved by 15 to 20 percent, the seedling days are also improved by 1 to 2 days compared with that of comparative example 1, the manual investment for shelling the scions is reduced, the damage rate of cotyledons of the scions is reduced, and meanwhile, the energy consumption for cultivating the scions is reduced due to the reduction of the seedling days, so that the cost and the labor are saved, and the cost of the watermelon grafted seedlings is further reduced.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.
Claims (1)
1. A method for promoting the graft scion shelling of watermelon grafted seedlings is characterized by comprising the following steps:
step 1: soaking watermelon seeds in warm soup at 55 ℃ for 30 minutes,
step 2: after the seed soaking, the seed soaking solution is placed in a gibberellin solution with the concentration of 800ppm for soaking for 4 hours, and then the seed soaking solution is cleaned and drained,
and step 3: wrapping the whole course of the drained watermelon seeds with a sterile towel or gauze, accelerating germination at 30 ℃,
and 4, step 4: sowing the germinated watermelon seeds in a flat-bottom plate, covering the flat-bottom plate with river sand with the thickness of 0.5cm, continuously accelerating germination for 4 days at the temperature of 30 ℃, wherein the matrix is formed by compounding peat and perlite according to the volume ratio of 3:1 until the height of the seedlings is 10cm, and the seedlings can be used for scion grafting of the watermelon.
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