CN113906133A - 工程改造的iPSC和免疫效应细胞中的CD3重建 - Google Patents

工程改造的iPSC和免疫效应细胞中的CD3重建 Download PDF

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CN113906133A
CN113906133A CN202080031737.3A CN202080031737A CN113906133A CN 113906133 A CN113906133 A CN 113906133A CN 202080031737 A CN202080031737 A CN 202080031737A CN 113906133 A CN113906133 A CN 113906133A
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cell
tcr
cells
human
tgcd3
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B·瓦拉梅尔
T·T·李
A·威蒂
M·普利巴蒂
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Fate Therapeutics Inc
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Fate Therapeutics Inc
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CN202080031737.3A 2019-04-11 2020-04-08 工程改造的iPSC和免疫效应细胞中的CD3重建 Pending CN113906133A (zh)

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US201962832622P 2019-04-11 2019-04-11
US62/832,622 2019-04-11
PCT/US2020/027327 WO2020210398A1 (en) 2019-04-11 2020-04-08 CD3 RECONSTITUTION IN ENGINEERED iPSC AND IMMUNE EFFECTOR CELLS

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CN (1) CN113906133A (https=)
AU (1) AU2020271523A1 (https=)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528453A (zh) * 2021-07-07 2021-10-22 金宇保灵生物药品有限公司 一种永生化猪巨噬细胞株及其构建方法和应用

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11629340B2 (en) 2017-03-03 2023-04-18 Obsidian Therapeutics, Inc. DHFR tunable protein regulation
SG11202104524YA (en) 2018-11-01 2021-05-28 Gracell Biotechnologies Shanghai Co Ltd Compositions and methods for t cell engineering
AU2021292695A1 (en) 2020-06-19 2023-02-02 Fate Therapeutics, Inc. Combining iPSC-derived effector cell types for immunotherapy use
CN114525259B (zh) * 2020-11-03 2025-02-14 南京北恒生物科技有限公司 靶向cd7的嵌合抗原受体及其用途
JP2023548467A (ja) * 2020-11-04 2023-11-17 フェイト セラピューティクス,インコーポレイティド 操作されたiPSC及び持続性免疫エフェクター細胞
JP2023549098A (ja) * 2020-11-04 2023-11-22 フェイト セラピューティクス,インコーポレイティド 固形腫瘍を標的とする多重操作されたiPSC及び免疫エフェクター細胞
US11591381B2 (en) 2020-11-30 2023-02-28 Crispr Therapeutics Ag Gene-edited natural killer cells
AU2021392032A1 (en) 2020-12-03 2023-06-22 Century Therapeutics, Inc. Genetically engineered cells and uses thereof
US11661459B2 (en) 2020-12-03 2023-05-30 Century Therapeutics, Inc. Artificial cell death polypeptide for chimeric antigen receptor and uses thereof
AR124414A1 (es) 2020-12-18 2023-03-22 Century Therapeutics Inc Sistema de receptor de antígeno quimérico con especificidad de receptor adaptable
CA3204417A1 (en) * 2021-01-29 2022-08-04 Allogene Therapeutics, Inc. Knockdown or knockout of one or more of tap2, nlrc5, ?2m, trac, rfx5, rfxap and rfxank to mitigate t cell recognition of allogeneic cell products
CN117377756A (zh) * 2021-03-05 2024-01-09 菲克特生物科学股份有限公司 工程化免疫细胞疗法
GB202110363D0 (en) * 2021-07-19 2021-09-01 Celyad S A NKG2D CAR cells expressing IL-18 for adoptive cell therapy
EP4504221A4 (en) * 2022-03-22 2026-02-25 Celyntra Therapeutics Sa Compositions and methods for generating cells with reduced immunogenicity
WO2023233342A2 (en) * 2022-06-01 2023-12-07 Crispr Therapeutics Ag Gene-edited natural killer cells
US20260071238A1 (en) * 2022-09-02 2026-03-12 Janssen Biotech, Inc. Materials and processes for engineering hypoimmunogenicity
WO2025076485A1 (en) * 2023-10-05 2025-04-10 Mink Therapeutics, Inc. Novel invariant natural killer t cells and t cell engager compositions and methods of use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170044500A1 (en) * 2014-04-24 2017-02-16 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
WO2018115887A1 (en) * 2016-12-21 2018-06-28 Ucl Business Plc Therapeutic cells
CN108368520A (zh) * 2015-11-04 2018-08-03 菲特治疗公司 多能细胞的基因组工程改造

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2014248119B2 (en) * 2013-04-03 2019-06-20 Memorial Sloan-Kettering Cancer Center Effective generation of tumor-targeted T-cells derived from pluripotent stem cells
WO2016007570A2 (en) * 2014-07-09 2016-01-14 The Regents Of The University Of California Engineered invariant natural killer t (inkt) cells and methods of making and using thereof
GB201506642D0 (en) * 2015-04-20 2015-06-03 Ucl Business Plc T cell receptor
CN117070468A (zh) * 2015-10-05 2023-11-17 精密生物科学公司 包含经修饰的人T细胞受体α恒定区基因的经遗传修饰的细胞
US11377637B2 (en) * 2016-04-15 2022-07-05 Memorial Sloan Kettering Cancer Center Transgenic T cell and chimeric antigen receptor T cell compositions and related methods
US20210015859A1 (en) * 2017-12-08 2021-01-21 Fate Therapeutics, Inc. IMMUNOTHERAPIES USING ENHANCED iPSC DERIVED EFFECTOR CELLS
CA3093449A1 (en) * 2018-03-09 2019-09-12 TCR2 Therapeutics Inc. Compositions and methods for tcr reprogramming using fusion proteins

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20170044500A1 (en) * 2014-04-24 2017-02-16 Board Of Regents, The University Of Texas System Application of induced pluripotent stem cells to generate adoptive cell therapy products
CN108368520A (zh) * 2015-11-04 2018-08-03 菲特治疗公司 多能细胞的基因组工程改造
WO2018115887A1 (en) * 2016-12-21 2018-06-28 Ucl Business Plc Therapeutic cells

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
TORIKAI,H. 等: "A foundation for universal T-cell based immunotherapy: T cells engineered to express a CD19-specific chimeric-antigen-receptor and eliminate expression of endogenous TCR", BLOOD, vol. 119, no. 34, pages 5697 - 5705 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113528453A (zh) * 2021-07-07 2021-10-22 金宇保灵生物药品有限公司 一种永生化猪巨噬细胞株及其构建方法和应用

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