CN113893197A - Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof - Google Patents
Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof Download PDFInfo
- Publication number
- CN113893197A CN113893197A CN202111273771.4A CN202111273771A CN113893197A CN 113893197 A CN113893197 A CN 113893197A CN 202111273771 A CN202111273771 A CN 202111273771A CN 113893197 A CN113893197 A CN 113893197A
- Authority
- CN
- China
- Prior art keywords
- skin
- skin whitening
- composition
- glycation
- soluble
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000002087 whitening effect Effects 0.000 title claims abstract description 78
- 239000000203 mixture Substances 0.000 title claims abstract description 76
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 239000000017 hydrogel Substances 0.000 claims abstract description 37
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 claims abstract description 21
- QRYRORQUOLYVBU-VBKZILBWSA-N Carnosic acid Natural products CC([C@@H]1CC2)(C)CCC[C@]1(C(O)=O)C1=C2C=C(C(C)C)C(O)=C1O QRYRORQUOLYVBU-VBKZILBWSA-N 0.000 claims abstract description 14
- 108010087806 Carnosine Proteins 0.000 claims abstract description 14
- CQOVPNPJLQNMDC-UHFFFAOYSA-N N-beta-alanyl-L-histidine Natural products NCCC(=O)NC(C(O)=O)CC1=CN=CN1 CQOVPNPJLQNMDC-UHFFFAOYSA-N 0.000 claims abstract description 14
- CQOVPNPJLQNMDC-ZETCQYMHSA-N carnosine Chemical compound [NH3+]CCC(=O)N[C@H](C([O-])=O)CC1=CNC=N1 CQOVPNPJLQNMDC-ZETCQYMHSA-N 0.000 claims abstract description 14
- 229940044199 carnosine Drugs 0.000 claims abstract description 14
- CSHZYWUPJWVTMQ-UHFFFAOYSA-N 4-n-Butylresorcinol Chemical compound CCCCC1=CC=C(O)C=C1O CSHZYWUPJWVTMQ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000000034 method Methods 0.000 claims abstract description 10
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 8
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 8
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 8
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 36
- 238000003756 stirring Methods 0.000 claims description 30
- 239000002537 cosmetic Substances 0.000 claims description 29
- 229920002385 Sodium hyaluronate Polymers 0.000 claims description 24
- 239000007788 liquid Substances 0.000 claims description 24
- 229940010747 sodium hyaluronate Drugs 0.000 claims description 24
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 claims description 24
- 239000002313 adhesive film Substances 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 235000011187 glycerol Nutrition 0.000 claims description 13
- 239000004745 nonwoven fabric Substances 0.000 claims description 11
- 238000001035 drying Methods 0.000 claims description 9
- 230000003796 beauty Effects 0.000 claims description 8
- 230000001681 protective effect Effects 0.000 claims description 7
- 239000011248 coating agent Substances 0.000 claims description 6
- 238000000576 coating method Methods 0.000 claims description 6
- 229920002134 Carboxymethyl cellulose Polymers 0.000 claims description 5
- FEWJPZIEWOKRBE-UHFFFAOYSA-N Tartaric acid Natural products [H+].[H+].[O-]C(=O)C(O)C(O)C([O-])=O FEWJPZIEWOKRBE-UHFFFAOYSA-N 0.000 claims description 5
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 5
- 239000001768 carboxy methyl cellulose Substances 0.000 claims description 5
- 235000010948 carboxy methyl cellulose Nutrition 0.000 claims description 5
- 229920001495 poly(sodium acrylate) polymer Polymers 0.000 claims description 5
- NNMHYFLPFNGQFZ-UHFFFAOYSA-M sodium polyacrylate Chemical compound [Na+].[O-]C(=O)C=C NNMHYFLPFNGQFZ-UHFFFAOYSA-M 0.000 claims description 5
- 235000002906 tartaric acid Nutrition 0.000 claims description 5
- 239000011975 tartaric acid Substances 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 235000007866 Chamaemelum nobile Nutrition 0.000 claims description 3
- 244000042664 Matricaria chamomilla Species 0.000 claims description 3
- 235000007232 Matricaria chamomilla Nutrition 0.000 claims description 3
- 240000001341 Reynoutria japonica Species 0.000 claims description 3
- 235000018167 Reynoutria japonica Nutrition 0.000 claims description 3
- 240000004534 Scutellaria baicalensis Species 0.000 claims description 3
- 235000017089 Scutellaria baicalensis Nutrition 0.000 claims description 3
- BWZOPYPOZJBVLQ-UHFFFAOYSA-K aluminium glycinate Chemical compound O[Al+]O.NCC([O-])=O BWZOPYPOZJBVLQ-UHFFFAOYSA-K 0.000 claims description 3
- 229940059958 centella asiatica extract Drugs 0.000 claims description 3
- 239000001331 rosmarinus officinalis leaf Substances 0.000 claims description 3
- 229940092665 tea leaf extract Drugs 0.000 claims description 3
- GULNRVTWPJODDE-UHFFFAOYSA-K aluminum;2-(dihydroxyamino)acetate Chemical compound [Al+3].ON(O)CC([O-])=O.ON(O)CC([O-])=O.ON(O)CC([O-])=O GULNRVTWPJODDE-UHFFFAOYSA-K 0.000 claims description 2
- 239000000835 fiber Substances 0.000 claims description 2
- 239000002985 plastic film Substances 0.000 claims description 2
- 229920006255 plastic film Polymers 0.000 claims description 2
- 229920000642 polymer Polymers 0.000 claims description 2
- 230000001815 facial effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 32
- 102000003425 Tyrosinase Human genes 0.000 abstract description 10
- 108060008724 Tyrosinase Proteins 0.000 abstract description 10
- 230000006870 function Effects 0.000 abstract description 7
- 238000010521 absorption reaction Methods 0.000 abstract description 6
- 230000008099 melanin synthesis Effects 0.000 abstract description 6
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 238000005282 brightening Methods 0.000 abstract description 5
- -1 carbohydrate compounds Chemical class 0.000 abstract description 5
- 150000001720 carbohydrates Chemical class 0.000 abstract description 5
- 230000008569 process Effects 0.000 abstract description 4
- 239000003053 toxin Substances 0.000 abstract description 4
- 231100000765 toxin Toxicity 0.000 abstract description 4
- 108700012359 toxins Proteins 0.000 abstract description 4
- 210000003491 skin Anatomy 0.000 description 73
- 239000000243 solution Substances 0.000 description 18
- 239000000047 product Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 14
- 238000006206 glycosylation reaction Methods 0.000 description 12
- 230000013595 glycosylation Effects 0.000 description 11
- 108010005094 Advanced Glycation End Products Proteins 0.000 description 10
- XUMBMVFBXHLACL-UHFFFAOYSA-N Melanin Chemical compound O=C1C(=O)C(C2=CNC3=C(C(C(=O)C4=C32)=O)C)=C2C4=CNC2=C1C XUMBMVFBXHLACL-UHFFFAOYSA-N 0.000 description 8
- 235000000346 sugar Nutrition 0.000 description 8
- 235000014633 carbohydrates Nutrition 0.000 description 7
- 230000000052 comparative effect Effects 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- KNFLNGRLKALWRF-LDXSYGEZSA-N CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)CC1=CC=CC=C1 Chemical compound CSCC[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CCCCN)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](C(C)C)C(N)=O)CC1=CC=CC=C1 KNFLNGRLKALWRF-LDXSYGEZSA-N 0.000 description 6
- 230000002255 enzymatic effect Effects 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 108090000623 proteins and genes Proteins 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- 230000036252 glycation Effects 0.000 description 5
- 230000008859 change Effects 0.000 description 4
- 238000011156 evaluation Methods 0.000 description 4
- 230000035699 permeability Effects 0.000 description 4
- 239000004480 active ingredient Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 description 2
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 206010070834 Sensitisation Diseases 0.000 description 2
- 206010040880 Skin irritation Diseases 0.000 description 2
- 102000019197 Superoxide Dismutase Human genes 0.000 description 2
- 108010012715 Superoxide dismutase Proteins 0.000 description 2
- 102000008314 Type 1 Melanocortin Receptor Human genes 0.000 description 2
- 108010021428 Type 1 Melanocortin Receptor Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000004027 cell Anatomy 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000003405 preventing effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000008313 sensitization Effects 0.000 description 2
- 230000036556 skin irritation Effects 0.000 description 2
- 231100000475 skin irritation Toxicity 0.000 description 2
- 102100030310 5,6-dihydroxyindole-2-carboxylic acid oxidase Human genes 0.000 description 1
- 101710163881 5,6-dihydroxyindole-2-carboxylic acid oxidase Proteins 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 206010013786 Dry skin Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000628997 Flos Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- 244000258271 Galium odoratum Species 0.000 description 1
- 235000008526 Galium odoratum Nutrition 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 239000000637 Melanocyte-Stimulating Hormone Substances 0.000 description 1
- 108010007013 Melanocyte-Stimulating Hormones Proteins 0.000 description 1
- DFPAKSUCGFBDDF-UHFFFAOYSA-N Nicotinamide Chemical compound NC(=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-UHFFFAOYSA-N 0.000 description 1
- 230000002292 Radical scavenging effect Effects 0.000 description 1
- 230000006750 UV protection Effects 0.000 description 1
- 229930003427 Vitamin E Natural products 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 229940098773 bovine serum albumin Drugs 0.000 description 1
- 108091006003 carbonylated proteins Proteins 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010094 cellular senescence Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 239000007854 depigmenting agent Substances 0.000 description 1
- 210000004207 dermis Anatomy 0.000 description 1
- 210000002249 digestive system Anatomy 0.000 description 1
- VJNCICVKUHKIIV-UHFFFAOYSA-N dopachrome Chemical compound O=C1C(=O)C=C2NC(C(=O)O)CC2=C1 VJNCICVKUHKIIV-UHFFFAOYSA-N 0.000 description 1
- 235000006694 eating habits Nutrition 0.000 description 1
- 238000005265 energy consumption Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- IDGUHHHQCWSQLU-UHFFFAOYSA-N ethanol;hydrate Chemical compound O.CCO IDGUHHHQCWSQLU-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 210000002510 keratinocyte Anatomy 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 210000002752 melanocyte Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229960003966 nicotinamide Drugs 0.000 description 1
- 235000005152 nicotinamide Nutrition 0.000 description 1
- 239000011570 nicotinamide Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000008055 phosphate buffer solution Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000011514 reflex Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000009210 therapy by ultrasound Methods 0.000 description 1
- 230000009466 transformation Effects 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/0208—Tissues; Wipes; Patches
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/34—Alcohols
- A61K8/347—Phenols
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
- A61K8/494—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds with more than one nitrogen as the only hetero atom
- A61K8/4946—Imidazoles or their condensed derivatives, e.g. benzimidazoles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
- A61K8/735—Mucopolysaccharides, e.g. hyaluronic acid; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/52—Stabilizers
- A61K2800/522—Antioxidants; Radical scavengers
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/74—Biological properties of particular ingredients
- A61K2800/78—Enzyme modulators, e.g. Enzyme agonists
- A61K2800/782—Enzyme inhibitors; Enzyme antagonists
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0046—Solid microneedles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M37/00—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin
- A61M37/0015—Other apparatus for introducing media into the body; Percutany, i.e. introducing medicines into the body by diffusion through the skin by using microneedles
- A61M2037/0053—Methods for producing microneedles
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Botany (AREA)
- Emergency Medicine (AREA)
- Biotechnology (AREA)
- Medical Informatics (AREA)
- Anesthesiology (AREA)
- Heart & Thoracic Surgery (AREA)
- Hematology (AREA)
- Cosmetics (AREA)
Abstract
The invention discloses a soluble microneedle patch composition for skin whitening and anti-saccharification and a preparation method thereof. The micro-needle film-sticking composition comprises a base, a needle body positioned on the base and a hydrogel film with the functions of relieving and fixing the needle body, wherein the needle body is internally embedded with a skin whitening and anti-saccharification composition: 0.1-3% of glycyrrhiza glabra root extract, 0.1-2% of 4-butyl resorcinol, 10.1-3% of nonapeptide and 0.1-5% of carnosine. The composition provided by the invention can inhibit the activity of tyrosinase in the three processes of before, during and after melanin synthesis, and has multiple effects of whitening; meanwhile, active carbohydrate compounds are removed, carbohydrate toxins are removed, AGEs are prevented from generating multiple anti-glycation, and the skin is finally white and bright due to the multi-path synergistic effect. The whitening and anti-glycation composition is embedded in the hyaluronic acid soluble microneedle body, so that the composition can be directly supplied to deep skin through the skin cuticle, the percutaneous absorption efficiency is improved, the skin is relieved and continuously moisturized, and the effects of efficient whitening, brightening and skin color uniformization are achieved.
Description
Technical Field
The invention relates to a skin care product and a preparation method thereof, belongs to the technical field of daily chemicals, and particularly relates to a soluble microneedle film composition for skin whitening and anti-saccharification and beauty treatment and a preparation method thereof.
Background
The phenomenon of saccharification is the physiological reaction of the combination of protein in our body and sugar in the food and drink. Carbohydrates taken in every day are decomposed into glucose and fructose through a digestive system, then are transported to cells, and carry out complex chemical reactions under the action of enzymes to release energy to meet the requirements of human bodies.
In young age, the activity is large, the metabolism is normal, most of the ingested sugar is metabolized, and the sugar cannot be accumulated in the body. However, with the age, the pressure of life and work increases, and the abnormal life and eating habits and metabolic disorders are added, so that the ingested sugar cannot be metabolized immediately and can be accumulated in the body.
Free sugars react with collagen in the dermis without enzymatic action, forming reversible primary "glycosylation products" and then irreversible "advanced glycosylation end products (AGEs)" throughout the process, which is the "glycation reaction". The saccharified collagen turns yellow in color and crisp in texture, and the skin gradually turns yellow with the accumulation of large amounts of AGEs. In addition, AGEs can stimulate the AGEs receptor to promote melanin production.
Healthy and fair skin is a hot spot pursued by all people who love beauty at present, but the whitening skin care products on the market generally have the following problems:
the whitening mechanism is single: most whitening products only aim at inhibiting the activity of tyrosinase and cannot realize full-effect whitening;
secondly, the product has poor stability: the whitening components in most whitening products are directly added into the formula, so that the problems of color change, flavor change, layering, coarseness and the like of the products are easily caused;
③ the whitening efficiency is low: because the skin has a natural skin barrier, the whitening active ingredients are difficult to permeate into the bottom of the skin, the absorption rate of the active substances is low, and the whitening effect is poor;
fourthly, easily causing skin irritation: such as niacinamide, if added to skin care products at too high a concentration, it is likely to cause skin irritation, even inflammation.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention aims to provide the soluble microneedle patch composition for skin whitening and anti-glycation and beautifying, which is embedded in a hyaluronic acid soluble microneedle body, is beneficial to directly supplying the composition to deep skin through the horny layer of the skin, improves the percutaneous absorption efficiency, simultaneously relieves the continuous moisture retention of the skin, and brings the effects of efficient whitening, brightening and skin color uniformity.
The invention also aims to provide a preparation method of the soluble microneedle patch composition for skin whitening and anti-saccharification beauty, which is simple, low in energy consumption and production cost and easy to realize continuous mass production.
The invention is realized by adopting the following technical scheme.
A micro-needle film composition for skin whitening, anti-saccharification and soluble beauty treatment comprises a base, a needle body positioned on the base and a hydrogel film for relieving and fixing the needle body; the skin whitening and anti-saccharification composition is embedded in the needle body, and comprises the following components in percentage by weight:
0.1-3% of glycyrrhiza glabra root extract, 0.1-2% of 4-butyl resorcinol, 10.1-3% of nonapeptide and 0.1-5% of carnosine.
Preferably, the skin whitening and anti-glycation composition comprises the following components in percentage by weight based on the total weight of the microneedle:
0.1-1% of glycyrrhiza glabra root extract, 0.1-0.5% of 4-butyl resorcinol, 10.1-1% of nonapeptide and 0.1-2% of carnosine.
Preferably, the skin whitening and anti-saccharification composition further comprises the following components in percentage by weight:
5-15% of glycerin, 1-3% of sodium hyaluronate with low molecular weight and 7-9% of sodium hyaluronate with high molecular weight.
Preferably, the soluble cosmetic microneedle is conical, the main component is hyaluronic acid, the length of the needle head is 150-.
Preferably, the hydrogel adhesive film has 4 layers, and the components are 10-20.
The hydrogel adhesive film consists of non-woven fabric, hydrogel, active component layer and protecting film.
The non-woven fabric is non-woven fabric and is formed by solidifying high polymer slices, short fibers or filaments.
The hydrogel comprises the following specific components in percentage by weight of the hydrogel adhesive film: 5-15% of glycerin, 1-2% of sodium polyacrylate, 1-3% of aluminum dihydroxyglycinate, 0.5-2% of cellulose gum, 1-5% of tartaric acid, 5-15% of butanediol and 50-80% of water.
According to the total weight of the hydrogel adhesive film, the active component layer comprises the following specific components:
0.1-3% of centella asiatica extract, 0.2-2% of polygonum cuspidatum root extract, 0.2-3% of scutellaria baicalensis root extract, 0.1-2% of tea leaf extract, 0.2-3% of glycyrrhiza glabra root extract, 0.3-1% of chamomile flower extract and 0.1-2% of rosemary leaf extract.
The protective film is a white plastic film.
In another aspect, the invention provides a preparation method of a soluble microneedle patch composition for skin whitening and anti-glycation, which comprises the following steps:
1) preparation of viscous liquid:
adding the skin whitening and anti-saccharification composition into the balance of water, stirring and dissolving, adding 5-15% of glycerol, 1-3% of sodium hyaluronate (low molecular weight) and 7-9% of sodium hyaluronate (high molecular weight), continuously stirring to enable the mixture to be uniformly swelled, and centrifuging to remove bubbles to obtain the viscous liquid.
2) Preparation of soluble cosmetic microneedles:
and injecting the viscous liquid into a microneedle mould, drying and demoulding to obtain the soluble cosmetic microneedle.
3) Preparation of hydrogel adhesive film:
uniformly mixing 1-2% of sodium polyacrylate and 1-3% of dihydroxy aluminum glycinate, adding 5-15% of glycerol and 5-15% of butanediol, and fully stirring to obtain a phase A solution;
adding 0.5-2% cellulose gum into water, stirring, adding 1-5% tartaric acid, adding active component layer, stirring to dissolve completely to obtain B phase solution;
and adding the B phase solution into the A phase solution, stirring in vacuum to obtain hydrogel paste, and finally coating the hydrogel paste on a coating machine together with a non-woven fabric and a protective film to obtain the hydrogel adhesive film.
Preferably, in the step 1), the molecular weight of the low molecular weight sodium hyaluronate is 2000-10000 daltons; the molecular weight of the high molecular weight sodium hyaluronate is 40-100 ten thousand daltons.
The mass ratio of the high molecular weight sodium hyaluronate to the low molecular weight sodium hyaluronate is 1: 3.0-5.0.
Preferably, in step 2), the microneedle mould may have a square, rectangular or arc shape, and may be a relatively expensive mask type.
Preferably, the drying temperature is room temperature, the humidity is 15-25%, and the drying time is 12-24 h.
Preferably, in the step 3), the stirring speed of the vacuum stirring is 50-120r/min, and the stirring time is 5-15 min.
By means of the technical scheme, the invention has the following beneficial technical effects:
1) the invention discloses a soluble microneedle patch composition for skin whitening and anti-saccharification and a preparation method thereof. The micro-needle film-sticking composition comprises a base, a needle body positioned on the base and a hydrogel film with the functions of relieving and fixing the needle body, wherein the needle body is internally embedded with a skin whitening and anti-saccharification composition: 0.1-3% of glycyrrhiza glabra root extract, 0.1-2% of 4-butyl resorcinol, 10.1-3% of nonapeptide and 0.1-5% of carnosine.
2) The composition provided by the invention can inhibit the activity of tyrosinase in the three processes of before, during and after melanin synthesis, and has multiple effects of whitening; meanwhile, active carbohydrate compounds are removed, carbohydrate toxins are removed, AGEs are prevented from generating multiple anti-glycation, and the skin is finally white and bright due to the multi-path synergistic effect.
3) The whitening and anti-glycation composition is embedded in the hyaluronic acid soluble microneedle body, so that the composition can be directly supplied to deep skin through the skin cuticle, the percutaneous absorption efficiency is improved, the skin is relieved and continuously moisturized, and the effects of efficient whitening, brightening and skin color uniformization are achieved.
Drawings
Fig. 1 is a structural diagram of a soluble cosmetic microneedle for skin whitening and anti-glycation provided by the invention.
Fig. 2 is a graph comparing the permeability results of the soluble cosmetic microneedles and the effective ingredients of the external skin care products.
Detailed Description
The technical solution of the present invention is further specifically described below by way of specific examples. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
As shown in the structural diagram of the soluble cosmetic microneedle of fig. 1, the invention discloses a soluble cosmetic microneedle patch composition for skin whitening and anti-glycation and a preparation method thereof. The micro-needle film-sticking composition comprises a base, a needle body positioned on the base and a hydrogel film with the functions of relieving and fixing the needle body, wherein a skin whitening and anti-saccharification composition is embedded in the needle body, and the composition comprises:
0.1-3% of glycyrrhiza glabra root extract, 0.1-2% of 4-butyl resorcinol, 10.1-3% of nonapeptide and 0.1-5% of carnosine.
The skin whitening and anti-saccharification soluble cosmetic microneedle patch composition comprises a base, a needle body positioned on the base and a hydrogel patch. The base is composed of hyaluronic acid; the microneedle body contains viscous liquid; the hydrogel adhesive film consists of non-woven fabric, hydrogel, active component layer and protecting film. The non-woven fabric has the functions of improving the air permeability of the film, the hydrogel body has the function of locking water for a long time, the active component layer has the functions of slowly releasing water, assisting in moisture preservation and relieving irritation and sensitivity, and the protective film has the function of preventing nutrition and water loss of the gel.
As shown in the comparison of the permeability results of the active ingredients of the soluble cosmetic micro-needle and the external skin care product in fig. 2, the permeability of the active ingredients of the soluble micro-needle technology is 4-6 times that of the common smearing type skin care product within 90 minutes.
The following description focuses on the efficacy of the raw materials of the composition.
Extract of glycyrrhiza glabra root
Glycyrrhiza glabra root extract, which penetrates deeply into the skin and maintains high activity, inhibits tyrosinase activity and dopachrome interconversion and DHICA oxidase activity, has oxygen radical scavenging ability similar to SOD (superoxide dismutase), has oxygen radical resisting ability similar to vitamin E, and also has skin roughness preventing, anti-inflammatory and antibacterial effects, and Glycyrrhiza glabra root extract used as a natural whitening agent is a component which has only been used so far as having a direct effect in 3 stages out of 5 stages of melanin formation.
2- (4-Butylresorcinol)
The 4-butyl resorcinol is a high-efficiency skin whitening agent, can effectively inhibit tyrosinase, reduce the formation of melanin, interfere the synthesis and glycosylation of the tyrosinase, improve the degradation of the tyrosinase and inhibit the transformation of the melanin to keratinocytes, and also has the advantages of oxidation resistance, ultraviolet resistance, quick penetration, low sensitization and the like.
③ nonapeptide-1
Nonapeptide-1 has good matching property with MC1 receptor on melanocyte, can be used as antagonistic agent of melanocyte stimulating hormone, competitively binds with MC1 receptor, prevents tyrosinase from being further activated to generate melanin, reduces melanin production, and can obviously brighten skin color.
Tetra (carnosine)
The research proves that the carnosine has good anti-glycation effect. Carbonylated proteins and glycation end products (AGEs) are signals for cellular senescence. They accumulate abnormally in cells, accelerating cross-linking with other proteins. Carnosine can react with sugar instead of protein in vivo to react with sugar before the sugar erodes skin, thereby helping to protect protein from glycosylation and achieving the effect of resisting glycation.
Therefore, the composition formed by combining the four components not only inhibits the activity of tyrosinase before, during and after the synthesis of melanin, but also has multiple effects of whitening; meanwhile, active carbohydrate compounds are removed, carbohydrate toxins are removed, AGEs are prevented from generating multiple anti-glycation, and the skin is finally white and bright due to the multi-path synergistic effect.
The skin whitening and anti-glycation composition is embedded in the hyaluronic acid soluble microneedle body, so that the composition can be directly supplied to deep skin through the skin cuticle, the percutaneous absorption efficiency is improved, the skin is relieved and continuously moisturized, and the effects of efficient whitening and brightening and uniform skin color are achieved.
The following percentage contents are all weight percentage contents. All the raw material components of the invention are commercial products.
The Glycyrrhiza glabra root extract is prepared by cleaning Glycyrrhiza glabra root, drying, and pulverizing to obtain Glycyrrhiza glabra root powder; mixing the powder, the crushed material for crushing the fine bubbles and deionized water, reacting for 4-8 hours at 70-80 ℃ to obtain a mixed solution, and performing ultrasonic treatment to obtain an extracting solution; and finally, passing the extracting solution through macroporous adsorption resin, eluting with water and 70% ethanol water solution respectively, and drying to obtain the glycyrrhiza glabra root extract.
Glycyrrhiza glabra root extract is purchased from Bolus for pharmaceutical use; 4-butyl resorcinol and carnosine were purchased from Dezhixin; nonapeptide-1 was purchased from Zhejiang surge. Centella asiatica extract, polygonum cuspidatum root extract, scutellaria baicalensis root extract, tea leaf extract, glycyrrhiza glabra root extract, chamomile flower extract, and rosemary leaf extract were obtained from Beijing university of Industrial and commercial sciences or Galium odoratum leaf Biotech Co.
Example 1
A preparation method of a soluble microneedle patch composition for skin whitening and anti-saccharification and beauty treatment comprises the following steps:
1) preparation of viscous liquid:
adding 0.6% of glycyrrhiza glabra root extract, 0.4% of 4-butyl resorcinol, 0.8% of nonapeptide-1 and 1.2% of carnosine into the balance of water, stirring and dissolving, adding 10% of glycerol, 2% of sodium hyaluronate (with the molecular weight of 5000 daltons) and 8% of sodium hyaluronate (with the molecular weight of 60 ten thousand daltons), continuously stirring to enable the mixture to be uniformly swelled, and centrifuging to remove bubbles to obtain the viscous liquid.
2) Preparation of soluble cosmetic microneedles:
and injecting the viscous liquid into a square microneedle mould, drying for 20 hours at room temperature under the condition that the humidity is 20%, and demoulding to obtain the soluble cosmetic microneedle.
3) Preparation of hydrogel adhesive film:
uniformly mixing 1.5% of sodium polyacrylate and 2% of dihydroxy aluminum glycinate, adding 10% of glycerol and 10% of butanediol, and fully stirring to obtain a phase A solution;
adding 1% cellulose gum into water, stirring, adding 3% tartaric acid, adding 0.5% herba Centellae extract, 0.6% rhizoma Polygoni Cuspidati extract, 0.4% radix Scutellariae extract, 0.5% folium Camelliae sinensis extract, 0.6% radix Glycytthizae extract, 0.8% flos Matricariae Chamomillae extract and 0.6% herba Rosmarini officinalis leaf extract, stirring to dissolve completely to obtain phase B solution;
and adding the B phase solution into the A phase solution, stirring in vacuum to obtain hydrogel paste, stirring at 80r/min for 10min, and finally coating on a coating machine together with a non-woven fabric and a protective film to obtain the hydrogel adhesive film.
Example 2
A preparation method of a soluble microneedle patch composition for skin whitening and anti-saccharification and beauty treatment comprises the following steps:
1) preparation of viscous liquid:
adding 0.4% of glycyrrhiza glabra root extract, 0.3% of 4-butyl resorcinol, 0.5% of nonapeptide-1 and 1% of carnosine into the balance of water, stirring and dissolving, adding 10% of glycerol, 2% of sodium hyaluronate (with the molecular weight of 5000 daltons) and 8% of sodium hyaluronate (with the molecular weight of 60 ten thousand daltons), continuously stirring to enable the mixture to be uniformly swelled, and centrifuging to remove bubbles to obtain the viscous liquid.
2) Preparation of soluble cosmetic microneedles: the same as in example 1.
3) Preparation of hydrogel adhesive film: the same as in example 1.
Example 3
A preparation method of a soluble microneedle patch composition for skin whitening and anti-saccharification and beauty treatment comprises the following steps:
1) preparation of viscous liquid:
adding 0.2% of glycyrrhiza glabra root extract, 0.1% of 4-butyl resorcinol, 0.3% of nonapeptide-1 and 0.5% of carnosine into the balance water, stirring and dissolving, adding 10% of glycerol, 2% of sodium hyaluronate (with the molecular weight of 5000 daltons) and 8% of sodium hyaluronate (with the molecular weight of 60 ten thousand daltons), continuously stirring to enable the mixture to be uniformly swelled, and centrifuging to remove bubbles to obtain the viscous liquid.
2) Preparation of soluble cosmetic microneedles: the same as in example 1.
3) Preparation of hydrogel adhesive film: the same as in example 1.
Blank example 1
A preparation method of a soluble cosmetic microneedle patch composition comprises the following steps:
1) preparation of viscous liquid:
adding 10% glycerol, 2% sodium hyaluronate (molecular weight of 5000 daltons) and 8% sodium hyaluronate (molecular weight of 60 ten thousand daltons) into the balance of water, stirring to make it swell uniformly, centrifuging to remove bubbles, and obtaining the viscous liquid.
2) Preparation of soluble cosmetic microneedles: the same as in example 1.
3) Preparation of hydrogel adhesive film: the same as in example 1.
Test example for Effect verification
Anti-glycation efficacy analysis experiment
Inhibition of non-enzymatic glycosylation studies:
enzymatic glycosylation is a complex series of non-enzymatic reactions, proteins and glucose which occur in vivo and ultimately form "aggressive" AGEs of skin glycation. Through research on non-enzymatic glycosylation reaction, the capacity of the skin whitening and anti-glycation composition for resisting glycation can be reflected.
Under aseptic conditions, 20.00g/L bovine serum albumin solution sterilized with a 0.2 μm sterilizing membrane and 5.00mL each of 0.50mol/L glucose solution were added to a sterile cell culture flask, and 1% NaN was added310.00mL of 0.20mol/L phosphate buffer solution with pH 7.4, and establishing a complete glycosylation system control group a; meanwhile, a control group b without adding viscous liquid containing the skin whitening and anti-saccharification composition and without adding glucose solution is set; adding a control group c containing a viscous liquid containing a skin whitening and anti-glycation composition and no protein; control group d containing viscous liquid containing skin whitening and anti-glycation composition without glucose solution.
The viscous liquids of examples 1 to 3 containing the skin whitening and anti-glycation compositions and the dry preparation of blank example 1 were set in a solution and incubated at a constant temperature of 37 ℃ for 28 days. The tests were carried out on days 0, 2, 4, 8, 12, 16, 20, 24, 26, 28, respectively.
0.5mL of culture solution is taken out, diluted to 10mL, the fluorescence value F of the glycosylation end product at the excitation wavelength of 370nm and the emission wavelength of 440nm is measured, and the inhibition rate IE of non-enzymatic glycosylation is calculated.
Formula for inhibition rate of non-enzymatic glycosylation IE: IE (%) - [1- (drug F-control cF-control dF)/(control aF-control bF) ] × 100%.
Wherein, the drug F is the fluorescence value of the sample to be tested, the control cF is the fluorescence value of the control group c, the control dF is the fluorescence value of the control group d, the control aF is the fluorescence value of the control group a, and the control bF is the fluorescence value of the control group b. After the glycosylated product is incubated, detecting the fluorescence intensity at 370nm/440nm, and calculating the inhibition rate, wherein the specific test results are shown in table 1:
TABLE 1 test results
| Item | Inhibition ratio% |
| Example 1 | 39.1 |
| Example 2 | 35.2 |
| Example 3 | 33.9 |
| Blank example 1 | 0.4 |
As can be seen from Table 1, the viscous liquids containing the skin whitening and anti-glycation compositions obtained in examples 1-3 of the present invention were able to inhibit nonenzymatic glycosylation well, and the inhibition IE for nonenzymatic glycosylation was 33% or more, whereas the viscous liquids containing no skin whitening and anti-glycation compositions obtained in blank example 1 were weak in inhibition ability. It is demonstrated that the skin whitening, anti-glycation composition has an anti-glycation effect.
Second, synergistic Experimental example
Comparative examples 1 to 14
A soluble cosmetic microneedle patch composition having a viscous liquid component as shown in tables 2-3 below.
TABLE 2 ingredient ratio (wt%)
TABLE 3 viscous liquid composition ratio (% by weight)
The preparation process is the same as that described in example 1.
The whitening effect test was performed on example 1, comparative examples 1 to 14, and blank example 1.
1. The tester: 160 healthy women (25-45 years old) divided into 16 groups of 10 persons each; each group used the same formulation.
2. Test area: face (left face blank example 1 sample, right face example, comparative example).
3. Use of: it is administered once daily for 1-2 hr for 4 weeks.
4. Testing an instrument: DelfinSkinColorCatch (finland).
5. The effect indexes are as follows: the change in color brightness (Δ L), ITA degree, and the L value (brightness) and ITA degree (color individual type angle) were measured and mainly used as indices.
Equation (1): Δ L ═ L (after 1 month of the test for the comparative or example products) -L (before the start of the test) ] - [ L ═ L (after 1 month of the test for the blank product) -L (before the start of the test).
(2): ITA degree (representing the overall change of chromaticity, the larger the ITA degree value, the lighter the skin color).
TABLE 4 skin color Classification for different ITA ° ranges
The test effects are shown in table 5 below.
TABLE 5 test results
As can be seen from the experimental data in table 5, the whitening effects are ranked as example 1> comparative examples 11, 12, 13, 14> comparative examples 5, 6, 7, 8, 9, 10> comparative examples 1, 2, 3, 4, and it is confirmed that the skin whitening, anti-glycation composition has better whitening effects through the synergistic effect between the components than the combination of only one, two or three components.
Third, human body trial evaluation experiment
The experimental method comprises the following steps: the skin whitening and anti-glycation soluble cosmetic microneedle patch compositions prepared in examples 1, 2 and 3 and blank example 1 were selected as experimental samples and respectively subjected to trial evaluation on volunteers. 80 volunteers with darker skin color and 40 men and women are selected and randomly divided into 4 groups of 10 men and 10 women.
Samples of the skin whitening, anti-glycation soluble cosmetic microneedle patch composition prepared in examples 1, 2, 3 and blank example 1 were used in 4 groups of volunteers, respectively. The using method comprises the following steps: the product is used after cleansing once a day for 1-2 hr for 4 weeks, and is subjected to sensory evaluation according to skin color improvement.
The scoring standard is 1 point-no effect, 2 points-effective and weak, 3 points-effective and obvious, 4 points-effective and better, and 5 points-effective and very good.
The score is more than or equal to 3, the effect is judged to be obvious, and the proportion of the score more than or equal to 3 in each group is counted.
The results obtained are shown in table 6:
TABLE 6 test results
| Evaluation results | Has obvious whitening effect | Has the effects of | Without effect |
| Example 1 | 85％ | 10％ | 5％ |
| Example 2 | 65％ | 25％ | 10％ |
| Example 3 | 45％ | 45％ | 10％ |
| Blank example 1 | 0 | 5％ | 95％ |
As can be seen from table 6, the soluble microneedle cosmetic film compositions for skin whitening and anti-glycation obtained in examples 1 to 3 of the present invention can effectively whiten skin, and have better effects than the soluble microneedle cosmetic film composition of the blank example 1 which does not contain a skin whitening and anti-glycation composition.
In conclusion, the skin whitening and anti-saccharification composition not only inhibits tyrosinase activity in the three processes before, during and after melanin synthesis, but also has multiple effects of whitening; meanwhile, active carbohydrate compounds are removed, carbohydrate toxins are removed, AGEs are prevented from generating multiple anti-glycation, and the skin is finally white and bright due to the multi-path synergistic effect.
The whitening and anti-glycation composition is embedded in the hyaluronic acid soluble microneedle body, so that the composition can be directly supplied to deep skin through the skin cuticle, the percutaneous absorption efficiency is improved, the skin is relieved and continuously moisturized, and the effects of efficient whitening, brightening and skin color uniformization are achieved.
The hydrogel patch is matched with the soluble microneedle for use, can release the moisture of the hydrogel patch in the absence of water, has self-adhesion and low sensitization, can cover the soluble cosmetic microneedle on the skin and is not easy to fall off, and ensures the stable exertion of the whitening and anti-saccharification effects of the microneedle.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention in any way, and other variations and modifications may be made without departing from the spirit of the invention as set forth in the claims.
Claims (10)
1. A microneedle patch composition for skin whitening, anti-saccharification and soluble beauty treatment is characterized in that: comprises a base, a needle body positioned on the base and a hydrogel adhesive film for relieving and fixing the needle body;
the skin whitening and anti-saccharification composition is embedded in the needle body, and comprises the following components in percentage by weight:
0.1-3% of glycyrrhiza glabra root extract, 0.1-2% of 4-butyl resorcinol, 10.1-3% of nonapeptide and 0.1-5% of carnosine.
2. The skin whitening, anti-glycation soluble cosmetic microneedle patch composition according to claim 1, wherein: the skin whitening and anti-saccharification composition comprises the following components in percentage by weight:
0.1-1% of glycyrrhiza glabra root extract, 0.1-0.5% of 4-butyl resorcinol, 10.1-1% of nonapeptide and 0.1-2% of carnosine.
3. The skin whitening, anti-glycation soluble cosmetic microneedle patch composition according to claim 1, wherein:
the skin whitening and anti-saccharification composition also comprises the following components in percentage by weight:
5-15% of glycerin, 1-3% of sodium hyaluronate with low molecular weight and 7-9% of sodium hyaluronate with high molecular weight.
4. The skin whitening, anti-glycation soluble cosmetic microneedle patch composition according to claim 1, wherein:
the soluble cosmetic microneedle is conical, the main component is hyaluronic acid, the length of the needle head is 150-.
5. The skin whitening, anti-glycation soluble cosmetic microneedle patch composition according to claim 1, wherein:
the hydrogel adhesive film has 4 layers, and the components are 10-20;
the hydrogel adhesive film consists of non-woven fabric, hydrogel, an active component layer and a protective film from top to bottom; the non-woven fabric is non-woven fabric and is formed by solidifying high polymer slices, short fibers or filaments;
the hydrogel comprises the following specific components in percentage by weight of the hydrogel adhesive film: 5-15% of glycerol, 1-2% of sodium polyacrylate, 1-3% of aluminum dihydroxyglycinate, 0.5-2% of cellulose gum, 1-5% of tartaric acid, 5-15% of butanediol and 50-80% of water;
according to the total weight of the hydrogel adhesive film, the active component layer comprises the following specific components:
0.1-3% of centella asiatica extract, 0.2-2% of polygonum cuspidatum root extract, 0.2-3% of scutellaria baicalensis root extract, 0.1-2% of tea leaf extract, 0.2-3% of glycyrrhiza glabra root extract, 0.3-1% of chamomile flower extract and 0.1-2% of rosemary leaf extract;
the protective film is a white plastic film.
6. The method for preparing the soluble microneedle patch composition for skin whitening and anti-glycation cosmetics according to any one of claims 1 to 5, comprising the steps of:
1) preparation of viscous liquid:
adding the skin whitening and anti-saccharification composition into the balance of water, stirring and dissolving, adding 5-15% of glycerol, 1-3% of sodium hyaluronate with low molecular weight and 7-9% of sodium hyaluronate with high molecular weight, continuing stirring to enable the mixture to be uniformly swelled, and centrifuging to remove bubbles to obtain viscous liquid;
2) preparation of soluble cosmetic microneedles:
injecting the viscous liquid into a microneedle mould, drying and demoulding to obtain a soluble cosmetic microneedle;
3) preparation of hydrogel adhesive film:
uniformly mixing 1-2% of sodium polyacrylate and 1-3% of dihydroxy aluminum glycinate, adding 5-15% of glycerol and 5-15% of butanediol, and fully stirring to obtain a phase A solution;
adding 0.5-2% cellulose gum into water, stirring, adding 1-5% tartaric acid, adding active component layer, stirring to dissolve completely to obtain B phase solution;
and adding the B phase solution into the A phase solution, stirring in vacuum to obtain hydrogel paste, and finally coating the hydrogel paste on a coating machine together with a non-woven fabric and a protective film to obtain the hydrogel adhesive film.
7. The method for preparing a soluble microneedle patch composition for skin whitening and anti-glycation according to claim 6, wherein:
in the step 1), the molecular weight of the low molecular weight sodium hyaluronate is 2000-10000 Dalton; the molecular weight of the high molecular weight sodium hyaluronate is 40-100 ten thousand daltons;
the mass ratio of the high molecular weight sodium hyaluronate to the low molecular weight sodium hyaluronate is 1: 3.0-5.0.
8. The method for preparing a soluble microneedle patch composition for skin whitening and anti-glycation according to claim 6, wherein:
in the step 2), the microneedle mould comprises a square shape, a rectangular shape or an arc shape, or is made into a facial mask type with higher cost.
9. The method for preparing a soluble microneedle patch composition for skin whitening and anti-glycation according to claim 6, wherein:
in the step 2), the drying temperature is room temperature, the humidity is 15-25%, and the drying time is 12-24 h.
10. The method for preparing a soluble microneedle patch composition for skin whitening and anti-glycation according to claim 6, wherein:
in the step 3), the stirring speed of the vacuum stirring is 50-120r/min, and the stirring time is 5-15 min.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111273771.4A CN113893197B (en) | 2021-10-29 | 2021-10-29 | Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111273771.4A CN113893197B (en) | 2021-10-29 | 2021-10-29 | Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113893197A true CN113893197A (en) | 2022-01-07 |
| CN113893197B CN113893197B (en) | 2024-01-30 |
Family
ID=79026980
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111273771.4A Active CN113893197B (en) | 2021-10-29 | 2021-10-29 | Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113893197B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115120536A (en) * | 2022-03-08 | 2022-09-30 | 佛山市谷邦生物科技有限公司 | Composition for improving skin color and inhibiting melanin migration and application thereof |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105310938A (en) * | 2015-11-18 | 2016-02-10 | 中山市艾丽意素生物科技有限公司 | Natural plant whitening cream and preparation method thereof |
| CN108272741A (en) * | 2018-03-30 | 2018-07-13 | 广州科盈化妆品有限公司 | A kind of anti-wrinkle essence breast and preparation method thereof |
| CN108542831A (en) * | 2018-07-05 | 2018-09-18 | 上海新高姿化妆品有限公司 | A kind of whitening and skin-protecting water and preparation method thereof with collaboration whitening effect |
| CN109568159A (en) * | 2018-12-05 | 2019-04-05 | 合肥卡迪尔生物科技有限公司 | Cosmetic composition and its preparation method and application is repaired in a kind of whitening |
| CN111481470A (en) * | 2020-06-09 | 2020-08-04 | 无锡元旭生物技术有限公司 | Hydrogel patch for soluble microneedle and preparation method thereof |
| CN111514087A (en) * | 2020-05-21 | 2020-08-11 | 上海江笙生物科技有限公司 | Whitening and skin-brightening composition and preparation method thereof |
| CN111743796A (en) * | 2020-05-26 | 2020-10-09 | 泉后(广州)生物科技研究院有限公司 | Whitening milk and preparation method thereof |
| CN111960924A (en) * | 2020-08-13 | 2020-11-20 | 常州大学 | Preparation method of 4-butyl resorcinol |
| CN112641656A (en) * | 2020-12-30 | 2021-04-13 | 宇肽生物(东莞)有限公司 | Polypeptide microneedle and preparation method thereof |
-
2021
- 2021-10-29 CN CN202111273771.4A patent/CN113893197B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105310938A (en) * | 2015-11-18 | 2016-02-10 | 中山市艾丽意素生物科技有限公司 | Natural plant whitening cream and preparation method thereof |
| CN108272741A (en) * | 2018-03-30 | 2018-07-13 | 广州科盈化妆品有限公司 | A kind of anti-wrinkle essence breast and preparation method thereof |
| CN108542831A (en) * | 2018-07-05 | 2018-09-18 | 上海新高姿化妆品有限公司 | A kind of whitening and skin-protecting water and preparation method thereof with collaboration whitening effect |
| CN109568159A (en) * | 2018-12-05 | 2019-04-05 | 合肥卡迪尔生物科技有限公司 | Cosmetic composition and its preparation method and application is repaired in a kind of whitening |
| CN111514087A (en) * | 2020-05-21 | 2020-08-11 | 上海江笙生物科技有限公司 | Whitening and skin-brightening composition and preparation method thereof |
| CN111743796A (en) * | 2020-05-26 | 2020-10-09 | 泉后(广州)生物科技研究院有限公司 | Whitening milk and preparation method thereof |
| CN111481470A (en) * | 2020-06-09 | 2020-08-04 | 无锡元旭生物技术有限公司 | Hydrogel patch for soluble microneedle and preparation method thereof |
| CN111960924A (en) * | 2020-08-13 | 2020-11-20 | 常州大学 | Preparation method of 4-butyl resorcinol |
| CN112641656A (en) * | 2020-12-30 | 2021-04-13 | 宇肽生物(东莞)有限公司 | Polypeptide microneedle and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115120536A (en) * | 2022-03-08 | 2022-09-30 | 佛山市谷邦生物科技有限公司 | Composition for improving skin color and inhibiting melanin migration and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113893197B (en) | 2024-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105963153B (en) | Active compound and cosmetics or skin care item comprising blue copper victory peptide | |
| CN103520081B (en) | The external use skin care of a kind of regulation of skin immunity, delay skin aging | |
| CN111388346A (en) | Freeze-dried powder live mask with vegetable protein and polypeptide formula and preparation method thereof | |
| CN103054771A (en) | Ultra-elastic water-locking facial mask essence and preparation method thereof | |
| CN113576975B (en) | Easily-absorbed anti-aging composition and preparation method and application thereof | |
| CN102274141B (en) | Herba selaginellae revival moisturizing cream and preparation method thereof | |
| CN104921961B (en) | A kind of degradable biological microneedle patch of multiple-effect reparation | |
| CN108078880A (en) | A kind of lightening compositions for including coix seed zymotic fluid | |
| CN110013447A (en) | A kind of cream and preparation method thereof for dispelling striae of pregnancy | |
| CN111265441A (en) | Composition capable of enhancing skin night repair and regeneration capacity and application thereof | |
| CN113893197B (en) | Skin whitening and anti-saccharification soluble beautifying microneedle patch composition and preparation method thereof | |
| CN110302128A (en) | A kind of whitening antiallergic rich in natural product extraction ingredient is releived sleep mask and preparation method thereof | |
| CN108670933B (en) | Skin care composition with moisturizing, whitening and anti-aging functions and application thereof | |
| CN105560092A (en) | Micro-repairing cream and preparation method thereof | |
| CN109431899A (en) | One kind 5 joins circle proferment pulp cosmetic and the preparation method and application thereof | |
| CN102228415B (en) | Composition containing tranexamic acid, and preparation method and uses thereof | |
| CN109260120A (en) | It is a kind of jade-like stone the flesh such as fine jade distribute ferment magma cosmetics and the preparation method and application thereof | |
| CN113908092A (en) | Skin acne removing and oil control soluble beautifying microneedle patch composition and preparation method thereof | |
| CN104546621A (en) | Anti-aging skincare composition and preparation method thereof | |
| CN111991319A (en) | Repair composition, preparation method and application thereof | |
| CN111658587A (en) | Collagenase inhibitor composition, anti-aging water lotion and preparation method thereof | |
| CN115518012B (en) | Composition with effects of inhibiting melanin protein and improving black eye and eye pouch and application thereof | |
| KR101435679B1 (en) | Composition for moisturizing and improving wrinkle on skin | |
| CN111700830B (en) | Composition and application thereof in preparation of anti-glycosylation cosmetics | |
| KR20170021278A (en) | Functional food composition comprising complex ferment consisting of soybean and barley |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |