CN113811618B - 基于甲基化dna目标区域构建测序文库及系统和应用 - Google Patents

基于甲基化dna目标区域构建测序文库及系统和应用 Download PDF

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CN113811618B
CN113811618B CN201980092935.8A CN201980092935A CN113811618B CN 113811618 B CN113811618 B CN 113811618B CN 201980092935 A CN201980092935 A CN 201980092935A CN 113811618 B CN113811618 B CN 113811618B
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sequence
primer
universal
sequencing
dna sample
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CN113811618A (zh
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杨林
张艳艳
王其伟
卢佳
陈芳
蒋慧
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MGI Tech Co Ltd
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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CN201980092935.8A 2019-05-21 2019-05-21 基于甲基化dna目标区域构建测序文库及系统和应用 Active CN113811618B (zh)

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CN114908151B (zh) * 2022-04-29 2026-02-03 上海伯杰医疗科技股份有限公司 用于基因cds区域一代测序的嵌套引物对、测序引物对、测序试剂、测序试剂盒及应用
CN115386966B (zh) * 2022-10-26 2023-03-21 北京寻因生物科技有限公司 Dna表观修饰的建库方法、测序方法及其建库试剂盒
WO2024119481A1 (zh) * 2022-12-09 2024-06-13 深圳华大智造科技股份有限公司 一种快速制备多重pcr测序文库的方法及其应用
WO2024124400A1 (zh) * 2022-12-13 2024-06-20 深圳华大智造科技股份有限公司 一种基于多重pcr的靶向甲基化建库体系、方法及其应用
WO2024259564A1 (zh) * 2023-06-19 2024-12-26 深圳华大智造科技股份有限公司 一种一步法构建靶向文库的方法及其应用
CN117316289B (zh) * 2023-09-06 2024-04-26 复旦大学附属华山医院 一种中枢神经系统肿瘤的甲基化测序分型方法及系统
CN117778578B (zh) * 2023-12-29 2024-10-15 深圳海普洛斯医学检验实验室 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用
CN118390167A (zh) * 2024-03-15 2024-07-26 臻赫医药(杭州)有限公司 高通量测序文库的构建方法、试剂盒及应用
CN118389494B (zh) * 2024-06-21 2025-06-20 北京寻因生物科技有限公司 细胞标签微珠及其制备方法和应用

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CN106011230A (zh) * 2016-05-10 2016-10-12 人和未来生物科技（长沙）有限公司 用于检测碎片化dna目标区域的引物组合物及其应用
CN107937985A (zh) * 2017-10-25 2018-04-20 人和未来生物科技（长沙）有限公司 一种微量碎片化dna甲基化检测文库的构建方法和检测方法

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US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
ES2922281T3 (es) * 2016-12-07 2022-09-12 Mgi Tech Co Ltd Método para construir una biblioteca de secuenciación de una célula individual y uso del mismo
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EP3950956A1 (en) 2022-02-09
JP2022525373A (ja) 2022-05-12
JP7203276B2 (ja) 2023-01-12
EP3950956A4 (en) 2022-05-04
CN113811618A (zh) 2021-12-17
US20220056519A1 (en) 2022-02-24

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