CN103131754B - 一种检测核酸羟甲基化修饰的方法及其应用 - Google Patents
一种检测核酸羟甲基化修饰的方法及其应用 Download PDFInfo
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- CN103131754B CN103131754B CN201110376589.1A CN201110376589A CN103131754B CN 103131754 B CN103131754 B CN 103131754B CN 201110376589 A CN201110376589 A CN 201110376589A CN 103131754 B CN103131754 B CN 103131754B
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
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- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
- C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
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- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
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Priority Applications (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201110376589.1A CN103131754B (zh) | 2011-11-24 | 2011-11-24 | 一种检测核酸羟甲基化修饰的方法及其应用 |
PCT/CN2012/084964 WO2013075629A1 (zh) | 2011-11-24 | 2012-11-21 | 一种检测核酸羟甲基化修饰的方法及其应用 |
US14/360,594 US9567633B2 (en) | 2011-11-24 | 2012-11-21 | Method for detecting hydroxylmethylation modification in nucleic acid and use thereof |
Applications Claiming Priority (1)
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CN201110376589.1A CN103131754B (zh) | 2011-11-24 | 2011-11-24 | 一种检测核酸羟甲基化修饰的方法及其应用 |
Publications (2)
Publication Number | Publication Date |
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CN103131754A CN103131754A (zh) | 2013-06-05 |
CN103131754B true CN103131754B (zh) | 2014-07-30 |
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CN201110376589.1A Active CN103131754B (zh) | 2011-11-24 | 2011-11-24 | 一种检测核酸羟甲基化修饰的方法及其应用 |
Country Status (3)
Country | Link |
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US (1) | US9567633B2 (zh) |
CN (1) | CN103131754B (zh) |
WO (1) | WO2013075629A1 (zh) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9115386B2 (en) | 2008-09-26 | 2015-08-25 | Children's Medical Center Corporation | Selective oxidation of 5-methylcytosine by TET-family proteins |
ES2872073T3 (es) | 2011-12-13 | 2021-11-02 | Univ Oslo Hf | Procedimientos y kits de detección de estado de metilación |
EP3351644B1 (en) | 2012-11-30 | 2020-01-29 | Cambridge Epigenetix Limited | Oxidising agent for modified nucleotides |
CN103388024B (zh) * | 2013-07-04 | 2015-06-17 | 徐州医学院 | 一种基于桥式pcr检测dna羟甲基化的方法 |
CN103409514B (zh) * | 2013-07-23 | 2016-01-06 | 徐州医学院 | 一种基于芯片的高通量高灵敏检测5-羟甲基化胞嘧啶的方法 |
CN103911439A (zh) * | 2014-03-13 | 2014-07-09 | 眭维国 | 系统性红斑狼疮羟甲基化状态的差异表达基因的分析方法和应用 |
CN104480214B (zh) * | 2014-12-30 | 2018-01-16 | 深圳市易基因科技有限公司 | 羟甲基化暨甲基化长序列标签测序技术 |
EP3355939A4 (en) | 2015-09-30 | 2019-04-17 | Trustees of Boston University | MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES |
EP3170883B1 (en) * | 2015-11-20 | 2021-08-11 | The Procter & Gamble Company | Cleaning product |
CN105648537B (zh) * | 2016-03-02 | 2018-06-29 | 上海易毕恩基因科技有限公司 | Dna5-甲基胞嘧啶与5-羟甲基胞嘧啶基因图谱测序方法 |
US11162139B2 (en) | 2016-03-02 | 2021-11-02 | Shanghai Epican Genetech Co. Ltd. | Method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine |
AU2017312953A1 (en) * | 2016-08-16 | 2019-01-24 | The Regents Of The University Of California | Method for finding low abundance sequences by hybridization (flash) |
CN107142320B (zh) * | 2017-06-16 | 2021-03-09 | 上海易毕恩基因科技有限公司 | 用于检测肝癌的基因标志物及其用途 |
WO2019051484A1 (en) * | 2017-09-11 | 2019-03-14 | Ludwig Institute For Cancer Research Ltd | SELECTIVE MARKING OF 5-METHYLCYTOSINE IN CIRCULATING ACELLULAR DNA |
CN109097460A (zh) * | 2018-08-30 | 2018-12-28 | 青岛大学 | 一种氧化修饰的含氮碱基的检测方法 |
CN109321647A (zh) * | 2018-10-26 | 2019-02-12 | 苏州森苗生物科技有限公司 | 标记组合物及羟甲基化核酸文库的构建方法 |
CN109811037B (zh) * | 2018-11-15 | 2022-02-11 | 华南师范大学 | 一种dna甲基化过程的连续在线检测方法 |
CN111254144B (zh) * | 2020-01-23 | 2023-04-28 | 南开大学 | 一种分子尺发夹结构及其用于测量单分子磁镊空间尺度准确性的方法 |
CA3187549A1 (en) | 2020-07-30 | 2022-02-03 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
CN115386966B (zh) * | 2022-10-26 | 2023-03-21 | 北京寻因生物科技有限公司 | Dna表观修饰的建库方法、测序方法及其建库试剂盒 |
Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011000721A1 (en) * | 2009-07-03 | 2011-01-06 | Universita' Degli Studi Di Milano | Inhibitors of microbial infections |
WO2011025819A1 (en) * | 2009-08-25 | 2011-03-03 | New England Biolabs, Inc. | Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation |
WO2011127136A1 (en) * | 2010-04-06 | 2011-10-13 | University Of Chicago | Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc) |
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2011
- 2011-11-24 CN CN201110376589.1A patent/CN103131754B/zh active Active
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2012
- 2012-11-21 WO PCT/CN2012/084964 patent/WO2013075629A1/zh active Application Filing
- 2012-11-21 US US14/360,594 patent/US9567633B2/en active Active
Patent Citations (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2011000721A1 (en) * | 2009-07-03 | 2011-01-06 | Universita' Degli Studi Di Milano | Inhibitors of microbial infections |
WO2011025819A1 (en) * | 2009-08-25 | 2011-03-03 | New England Biolabs, Inc. | Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation |
WO2011127136A1 (en) * | 2010-04-06 | 2011-10-13 | University Of Chicago | Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc) |
Non-Patent Citations (2)
Title |
---|
Chun-Xiao Song et al..Detection of 5-Hydroxymethylcytosine in a Combined.《Bioorg Med Chem Lett.》.2011,第21卷(第17期),第5075-5077页. * |
WA Pastor et al..Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells.《nature》.2011,第473卷(第7347期),第394-397页. * |
Also Published As
Publication number | Publication date |
---|---|
CN103131754A (zh) | 2013-06-05 |
US20150031552A1 (en) | 2015-01-29 |
WO2013075629A1 (zh) | 2013-05-30 |
US9567633B2 (en) | 2017-02-14 |
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