JP7203276B2 - メチル化されたｄｎａの標的領域に基づいてシーケンシングライブラリーを構築する方法及びキット - Google Patents

メチル化されたｄｎａの標的領域に基づいてシーケンシングライブラリーを構築する方法及びキット Download PDF

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JP7203276B2
JP7203276B2 JP2022502317A JP2022502317A JP7203276B2 JP 7203276 B2 JP7203276 B2 JP 7203276B2 JP 2022502317 A JP2022502317 A JP 2022502317A JP 2022502317 A JP2022502317 A JP 2022502317A JP 7203276 B2 JP7203276 B2 JP 7203276B2
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primer
universal
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sequencing
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JP2022525373A (ja
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楊林
張艷艷
王其偉
盧佳
陳芳
蒋慧
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MGI Tech Co Ltd
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    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6869—Methods for sequencing
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups

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JP2022502317A 2019-05-21 2019-05-21 メチル化されたｄｎａの標的領域に基づいてシーケンシングライブラリーを構築する方法及びキット Active JP7203276B2 (ja)

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PCT/CN2019/087824 WO2020232635A1 (zh) 2019-05-21 2019-05-21 基于甲基化dna目标区域构建测序文库及系统和应用

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JP2022525373A JP2022525373A (ja) 2022-05-12
JP2022525373A5 JP2022525373A5 (https=) 2022-11-18
JP7203276B2 true JP7203276B2 (ja) 2023-01-12

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US (1) US20220056519A1 (https=)
EP (1) EP3950956A4 (https=)
JP (1) JP7203276B2 (https=)
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CN114908151B (zh) * 2022-04-29 2026-02-03 上海伯杰医疗科技股份有限公司 用于基因cds区域一代测序的嵌套引物对、测序引物对、测序试剂、测序试剂盒及应用
CN115386966B (zh) * 2022-10-26 2023-03-21 北京寻因生物科技有限公司 Dna表观修饰的建库方法、测序方法及其建库试剂盒
WO2024119481A1 (zh) * 2022-12-09 2024-06-13 深圳华大智造科技股份有限公司 一种快速制备多重pcr测序文库的方法及其应用
WO2024124400A1 (zh) * 2022-12-13 2024-06-20 深圳华大智造科技股份有限公司 一种基于多重pcr的靶向甲基化建库体系、方法及其应用
WO2024259564A1 (zh) * 2023-06-19 2024-12-26 深圳华大智造科技股份有限公司 一种一步法构建靶向文库的方法及其应用
CN117316289B (zh) * 2023-09-06 2024-04-26 复旦大学附属华山医院 一种中枢神经系统肿瘤的甲基化测序分型方法及系统
CN117778578B (zh) * 2023-12-29 2024-10-15 深圳海普洛斯医学检验实验室 一种基于高通量测序检测目标基因甲基化程度的引物组合物及其应用
CN118390167A (zh) * 2024-03-15 2024-07-26 臻赫医药(杭州)有限公司 高通量测序文库的构建方法、试剂盒及应用
CN118389494B (zh) * 2024-06-21 2025-06-20 北京寻因生物科技有限公司 细胞标签微珠及其制备方法和应用

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2018103025A1 (zh) 2016-12-07 2018-06-14 深圳华大智造科技有限公司 单细胞测序文库的构建方法及其应用
US20190002872A1 (en) 2017-06-30 2019-01-03 Life Technologies Corporation Library preparation methods and compositions and uses therefor

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CN102796808B (zh) * 2011-05-23 2014-06-18 深圳华大基因科技服务有限公司 甲基化高通量检测方法
CN106011230A (zh) * 2016-05-10 2016-10-12 人和未来生物科技（长沙）有限公司 用于检测碎片化dna目标区域的引物组合物及其应用
CN105861724B (zh) * 2016-06-03 2019-07-16 人和未来生物科技（长沙）有限公司 一种kras基因超低频突变检测试剂盒
AU2018243360B2 (en) * 2017-03-29 2023-10-26 Cornell University Devices, processes, and systems for determination of nucleic acid sequence, expression, copy number, or methylation changes using combined nuclease, ligase, polymerase, and sequencing reactions
CN107937985A (zh) * 2017-10-25 2018-04-20 人和未来生物科技（长沙）有限公司 一种微量碎片化dna甲基化检测文库的构建方法和检测方法
CN107541791A (zh) * 2017-10-26 2018-01-05 中国科学院北京基因组研究所 血浆游离dna甲基化检测文库的构建方法、试剂盒及应用
CN109666720A (zh) * 2018-12-28 2019-04-23 北京中科遗传与生殖医学研究院有限责任公司 一种对胚胎培养液进行DedscRRBS-PGS分析的方法

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
WO2018103025A1 (zh) 2016-12-07 2018-06-14 深圳华大智造科技有限公司 单细胞测序文库的构建方法及其应用
US20190002872A1 (en) 2017-06-30 2019-01-03 Life Technologies Corporation Library preparation methods and compositions and uses therefor

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WO2020232635A1 (zh) 2020-11-26
EP3950956A1 (en) 2022-02-09
JP2022525373A (ja) 2022-05-12
CN113811618B (zh) 2024-02-09
EP3950956A4 (en) 2022-05-04
CN113811618A (zh) 2021-12-17
US20220056519A1 (en) 2022-02-24

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