CN113749021B - Method for cultivating sepia duchenne fries - Google Patents
Method for cultivating sepia duchenne fries Download PDFInfo
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- CN113749021B CN113749021B CN202111188726.9A CN202111188726A CN113749021B CN 113749021 B CN113749021 B CN 113749021B CN 202111188726 A CN202111188726 A CN 202111188726A CN 113749021 B CN113749021 B CN 113749021B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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Abstract
The invention discloses a method for cultivating sepia duchenne fries, and belongs to the field of aquaculture. The method comprises the steps of putting fertilized eggs of the cuttlefish in a sieve basin for hatching, and putting the hatched larva into a fry breeding pond with the breeding density of 800-1000 fries/m3(ii) a 1-6 d after hatching of fertilized eggs, adding new water 10cm every day, increasing the water level from 40cm to 1m, changing water from 7 days old, gradually increasing the water exchange amount from 40% to 200%, and slightly inflating; after 30 days of age, cultivating in running water, increasing the water change amount to more than 300%, and increasing the aeration amount; the water temperature is 20-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-32, the dissolved oxygen is more than 6mg/L, the plant is 60-70 days old, the trunk length is 2-2.5 cm, and then seedling emergence can be realized; the bait for hatching the young cuttlefish is mackerel ovum of embryo body lower bag 4/5-artemia nauplius or cladocera species-bran shrimp or Penaeus vannamei Boone. The survival rate of the offspring seed cultivation by the method reaches 60 percent.
Description
Technical Field
The invention relates to a method for cultivating sepia donovani fries, and belongs to the technical field of aquaculture.
Background
The squid is characterized in that the squid is a squid (Loligo duvacueli), the same-species different-name squid (Loligo oshimai), also called tubule, Daiseusia, shallow squid, belonging to the phylum mollusca (Mollusa), Cephalopoda (Cephalopoda), Mucuna (Teuthoidea), Murashike (Loliginidae) and Murashige (Lotignidae) and distributed in the south sea and the Islands of the Islands on Marie. The small-barreled spearhead cuttlefish is one of economic types in offshore spearhead inkfish families in China, is dense in groups, produces hundreds of tons of eggs annually, has development potential, is particularly famous in Fujian Dongshan island small canals in Zhangzhou, has the characteristics of crisp mouthfeel, fine and fresh meat quality and the like, and is a special seafood which is eaten by tourists to Dongshan island in each place. However, in recent years, due to the fact that a great amount of roe parents are caught, the resource amount is sharply reduced, and the price is continuously increased, so that research on breeding and breeding technologies of the sepiella adolescens is urgently needed to promote the recovery of the resources.
It is reported that there are 756 kinds of cephalopods, of which cuttlefish species living ashore and cuttlefish species living offshore, middle and lower layers are relatively easy to breed, and squid spears swimming quickly are relatively difficult to breed, while soft fishes living in the ocean and open sea are most difficult to breed. At present, varieties developed by breeding technologies at home and abroad are concentrated on cuttlefish and octopus, and the cuttlefish are not reported. In 2019, the research on the domestication and fry breeding technology of the sepia dobby was first developed by the yellow sea aquatic product research institute of the Chinese aquatic product science research institute, and a good foundation is laid for the development of the types of the calamus. However, in the breeding production, the breeding technology of the squid is different from the existing squid and octopus, the breeding difficulty is very high, for example, the initial baits of the squid and the octopus are generally artemia or mysorethorn, but because the squid is hatched to have small young squids and the individual artemia or mysorethorn is bigger, the rotifers with wide sources and smaller individual cannot meet the nutritional requirements of the squid under the nutritional condition, and in addition, because the rotifers have round body shapes, the catching of the larva of the squid is not facilitated, the existing baits are not suitable for the early-stage cultivation of the offspring seeds of the squid; in addition, because the squid does not swim fast, the inner shell is thin, and the rear end of the inner shell is extremely sharp, the tail of the squid is easy to fester due to collision with the wall of the pond and the rear end of the inner shell is broken to die, so that the survival rate of fry breeding is low, special breeding conditions are required to be set to prevent damage caused by collision with the wall of the pond, but the report of the squid fry breeding technology is not seen so far.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a method for cultivating the sepia donaiensis fries, which solves the problem of initial feed of the sepia donaiensis fries and can successfully cultivate healthy fries.
The invention is realized by the following technical scheme:
a method for cultivating Sepia Dolichoris fry comprises the following steps
1) Obtaining fertilized eggs of the cuttlefish in the Duchenne squid, putting the fertilized eggs into a sieve basin in an incubation pool for incubation, and disinfecting the fertilized eggs for 1-2 min by using 20-30 ppm iodophor solution every 2-3 days before the fries are incubated out; during the incubation period, the water temperature is 20-26 ℃, the water change amount is 300-400%, the air is continuously inflated, and the seedlings are incubated for 10-12 days in sequence;
2) transferring the fertilized eggs into a seedling cultivation pool for continuous incubation 1-2 days before the seedlings are incubated, after the seedlings are incubated, feeding the fertilized eggs into the seedling cultivation pool through a pot sieving hole or scooping the fertilized eggs out one by using water, counting and transferring the fertilized eggs into the seedling cultivation pool for cultivation, wherein the seedling cultivation pool is circular and has an area of 1-6 m3The height is 1.2-1.5 m, and the cultivation density is 800-1000 pieces/m3(ii) a Adding fresh water 10cm every day 1-6 days after the fertilized eggs are hatched, increasing the water level from 40cm to 1m, changing water from 7 days old, gradually increasing the water exchange amount from 40% to 200%, and simultaneously adopting a nano air pipe to oxygenate and slightly inflate; after 30 days of age, transferring the seedlings into 10-20 m3The circular cultivating pool is used for cultivating, water is grown, the water change amount is increased to more than 300%, and the aeration amount is increased; the cultivation water is used after precipitation and sand filtration, the water temperature is 20-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-32, the dissolved oxygen is more than 6mg/L, the plant is 60-70 days old, and the trunk length is 2-2.5 cm, so that seedling emergence can be realized;
after the young cuttlefishes are hatched successively, feeding the eggs of the mackerel with the embryo body lower bag 4/5 in a young cuttlefish breeding pond at one time, wherein the density is 8-10 g/m2Or directly feeding blue stone spot rudimental fry after the young cuttlefish is hatched for 1d, wherein the density is 3-5 fish/ml; feeding artemia nauplii or cladocerans with the density of 2-3 per ml at the age of 10-25 days; feeding the young shrimps or the penaeus vannamei boone after 20 days of age, wherein the density is 1-2/ml; feeding the mackerel roe once, and feeding the rest baits 2 times a day; EM bacteria and artemia shrimp slices are uniformly sprayed in the seedling pool every day to improve the water quality and the water color and further reduce the stress.
Further, the eggs of the mackerel are obtained by natural spawning, and the newly hatched larvae are hatched in a high density by adopting a hatching barrel or a hatching net cage, wherein the hatching density is 800-1000 g/m3Before feeding the cuttlefish, the cuttlefish is fished out by a mesh bag.
Further, 30-day-old Sepiella donovani fries are transferred into 10-20 m3When the cuttlefish seeds are poured into the culture pond, the water level is slowly reduced, then the cuttlefish larvae are driven into the washbasin by the diddle net, and the whole process is operated with water, so that the cuttlefish seeds are prevented from leaving water and jetting ink.
Further, the concentration of the EM bacteria is 8-10 ml/m3(ii) a When the artemia salina prawn slices are used, the artemia salina prawn slices are scrubbed by a 300-mesh net bag, and the concentration is 5-10 g/m3
Further, the fertilized eggs of the cuttlefish come from artificial cultivation, and the cultivation method comprises the following steps:
(1) harvesting of parents
Fishing the adult squid of the Dolichi gun in 7-9 months every year, and transferring the captured adult squid into a net cage or a cabin for temporary culture;
(2) transportation of parents
Transporting the captured parent to a wharf, weighing the parent with water, transferring the parent to a live fish transport vehicle, filling pure oxygen into the water vehicle by adopting nano air stones, adding 20-30 ppm of florfenicol and 8-10 ppm of aquatic product excitant into the transport water to reduce the occurrence of ink jet behaviors and prevent bacterial infection, wherein the transport density is 8-10 kg/m3The transportation time is not more than 1h, and the operation with water is carried out during loading and unloading so as to reduce the operation damage;
(3) temporary raising of parents
A circle of nano-air pipes are arranged at the bottom of the inner wall of the culture pond, an air curtain is formed during air inflation, the parents can be effectively prevented from colliding with the pond wall, air inflation and oxygenation are continuously performed, and the illumination intensity is controlled to be 400 lx-800 lx; the water temperature is 20-28 ℃, and the salinity is 25-32; a density of 5 to 8kg/m3(ii) a The running water cultivation is adopted, the water changing rate is 400-500%, and the interior of a workshop needs to be kept quiet; fresh and live baits are adopted for nutrient domestication, the daily feeding amount is 2% -3% of the weight of the cuttlefish, the cuttlefish is fed for 2 times every day, the residual baits and excrement are removed in the morning every day, and water is thoroughly changed; 3d after entering the pool, 10-20 ppm of florfenicol medicated bath is used for 1-2 h every day;
(4) collection of fertilized egg
The method is characterized in that a plastic plate with holes is used for surrounding a central pipe of a culture pond as a center and is cylindrical, the diameter of the plastic plate is 60-100 cm, the height of the plastic plate is 10-20 cm higher than that of the central pipe, the bottom of the culture pond is fixed, the hole diameter of the plastic plate is 1-2 cm, the hole distance is 2-3 mm, collected egg sheaths are picked off from the plastic plate with holes every day after a parent lays eggs, and the plastic plate with holes is placed into hatching equipment for hatching.
Compared with the prior art, the invention has the beneficial effects that:
1) the sepia gunnii seedlings are difficult to grow, and no breeding technical report is seen at home and abroad. Because the young squid hatchlings are smaller, the invention selects the blue-spotted newly hatched fries which are suitable in size, approximate in suitable water temperature, high in hatching and cultivating survival rate, cheap and easy to obtain as the cuttlefish initial feed, not only meets the nutritional requirements of the cuttlefish, but also is beneficial to the predation of the cuttlefish due to the slender fish bodies, and further improves the survival rate.
2) The cuttlefish in the present invention has fast swimming, thin inner shell and sharp back end, and is easy to damage and death; the early-stage micro-inflation reduces the collision caused by the impact of the airflow; EM bacteria are added into water to control the water quality, so that stress collision and ink jet caused by multiple bottom suction are avoided; in addition, the artemia salina prawn slices are splashed on the offspring seeds, the water color is adjusted to be brown, the stress caused by strong light is reduced, a perfect offspring seed cultivation technology is established, and important reference is provided for research and development of a gunyloid cephalopodium breeding technology.
The method can obtain healthy larvae, and the survival rate of fry culture reaches 60 percent.
Detailed Description
The invention is further explained by the embodiments, but the scope of protection of the invention is not limited in any way by the embodiments.
Example 1
(1) 620 cuttlefish parents were collected from the sea area. And (3) hatching the fertilized eggs in a sieve basin, wherein the diameter of the sieve basin is 30cm, the height of the sieve basin is 10cm, the diameter of a basin eye is 2mm, the water permeability is good, and ootheca is not exposed. And (3) sterilizing the fertilized eggs for 1-2 min by using 20ppm iodophor solution every 2-3 d before the offspring seeds are hatched, wherein the ootheca is thinner and thinner because the fertilized eggs are gradually expanded before hatching. And hatching the seedlings for 10-12 days at the water temperature of 24-26 ℃. During the hatching period, the water exchange amount is 300-400%, and the air is continuously inflated.
(2) Fry rearing
And transferring the fertilized eggs into a seedling cultivation pond for continuous incubation 1-2 days before the seedlings are incubated. After the larvae are hatched out, the larvae enter a fry rearing pond through a pot sieving hole, or are scooped out one by water, and are transferred into the fry rearing pond for rearing after counting. Because the young fries of the newly hatched larvae are small, the fry culture pond is not too large for convenient observation and management, and a circular pond is suitable for being adopted according to the circumferential swimming habit of the cuttlefish, therefore, a circular glass fiber reinforced plastic water tank or a small circular cement pond is adopted for culture in the early stage, and the area is 1-6 m3The area of the fry rearing pond is 2m in the embodiment3Height of 1.2m and cultivation density of 800 pieces/m3(ii) a And (3) hatching the young black-bone fish for 1-6 days, adding 10cm of fresh water every day, increasing the water level from 40cm to 1m, starting water change after 7 days of age, gradually increasing the water exchange amount from 40% to 200%, and simultaneously adopting a nano air pipe for oxygenation and micro-inflation to prevent the young bodies from colliding with the wall of the pool. After 30 days of age, transferring the seedlings to the area of 10m3The water exchange amount of the culture pond is increased to more than 300%, and meanwhile, the aeration amount is increased. After the young cuttlefish is hatched, the young cuttlefish is fed with an embryo bag 4/5 for one time, and the density of the eggs of the mackerel which are to be hatched to the membrane is 8-10 g/m2Or directly feeding blue stone spot rudimental fry after the young cuttlefish is hatched for 1d, wherein the density is 3-5 fish/ml; feeding artemia nauplii or cladocerans with the density of 2-3 per ml at the age of 10-25 days; feeding mythic fungus or penaeus vannamei boone after 20 days of age, wherein the density is 1-2/ml, the mackerel roe is fed once, and the rest baits are fed 2 times per day. In order to prevent ink jet of cuttlefish caused by bottom suction operation and light irradiation stress, EM bacteria (10 ml/m) can be uniformly sprinkled in the fry rearing pond every day without bottom suction3) And artemia salina prawn slices (washed by a 300-mesh net bag when in use, the concentration is 5-10 g/m3) To improve water quality and water color and further reduce stress. The cultivation water is used after precipitation and sand filtration, the water temperature is 22-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-29, and the dissolved oxygen is more than 6 mg/L. The trunk length can reach 2cm at about 60 days old, and can be used for proliferation, discharge and productionIndustrialized popularization and cultivation.
The survival rate of the cuttlefish fries cultured in the embodiment is 60%, and 4.2 ten thousand fries with 2cm trunk length are cultured.
Example 2
(1) Harvesting of parents
And 7-9 months in 2020, catching adult Dolichi gun cuttlefishes by using a special illumination platform or a small illumination ship, transferring the caught adult Dolichi gun cuttlefishes into a net cage or a cabin for temporary culture, and collecting 820 parents in total.
(2) Transportation of parents
The captured parents are transported to a wharf, weighed with water and transferred to a live fish transport vehicle. The waterwheel adopts nano-aerolith filled with pure oxygen, and 20ppm of florfenicol and 8ppm of aquatic excitant are added into the transportation water so as to reduce the occurrence of ink-jet behavior and prevent and treat bacterial infection. The transportation density is 8-10 kg/m3The transportation time is not more than 1h, and the operation with water is carried out during loading and unloading, so that the operation damage is reduced.
(3) Temporary raising of parents
A circle of nano-air pipes are arranged along the inner wall of the culture pond and tightly attached to the bottom of the pond, and an air curtain is formed when the nano-air pipes are inflated, so that the parents can be effectively prevented from colliding with the wall of the culture pond. The area of the domesticating and breeding pond is 20m2Continuously inflating and oxygenating when the water depth is 0.8-1.0 m, and controlling the illumination intensity to be 400-800 lx, uniform and soft; the water temperature is 24-26 ℃, and the salinity is 28-32; a density of 5 to 8kg/m3(ii) a The method adopts running water cultivation, the water change rate is 400-500%, and the interior of a workshop needs to be kept quiet. The method comprises the steps of carrying out nutrition domestication on fresh decapterus maruadsi or litopenaeus vannamei live shrimps and other high-quality baits with the length of 5-8 cm, feeding the decapterus maruadsi or litopenaeus vannamei 2-3% of the weight of the sepiella margaritae daily, preferably feeding the sepiella margarita and the litopenaeus margaritae 2 times daily, removing residual baits and excrement in the morning every day, and thoroughly changing water. 3d after the parents are in the pool, a 15ppm florfenicol medicated bath is used for 2h each day to treat handling injuries caused during fishing, weighing and transportation.
(4) Collection of fertilized egg
Aiming at the characteristics that the egg sheath of the sepia donovani is too long and is not firmly adhered, and the egg membrane of the egg sheath is easily broken due to the fact that the egg sheath is easily rotated to the central tube by water flow, and the like, a perforated plastic plate with the thickness of 1mm, the aperture of 1cm and the aperture distance of 2mm is adopted, the central tube is used as the center, the perforated plastic plate is enclosed into a cylinder shape, the diameter of the cylindrical plastic plate is 60cm, the height of the cylindrical plastic plate is 10cm higher than that of the central tube, and the bottom of the cylindrical plastic plate is fixed at the bottom of the pool. The attachment base can provide a plurality of egg hanging places, the sepiella peelii can hang eggs on the circular holes, the annular plastic plate with the holes equivalently enlarges the diameter of the central tube, and the phenomenon that the egg sheaths falling off during the water changing operation are screwed on the central tube is effectively avoided; in addition, the cuttlefish has cluster circumambulation habit, the normal swimming of the cuttlefish can be hindered by the existing cephalopod attaching bases, and the device does not influence the normal circumambulation of the cuttlefish, so that the activity space is enlarged, and the collision probability is reduced.
Collecting fertilized eggs of the cuttlefish in the morning from an annular plastic plate with holes, and hatching the fertilized eggs in a sieve basin with the diameter of 30cm, the height of 10cm and the diameter of 2mm, wherein the water permeability is good, and the ootheca is not exposed. Because the fertilized eggs gradually expand before hatching, the egg membranes become thinner and thinner, and 20ppm iodophor solution is used for disinfection for 1min every 2-3 days before the offspring seeds are hatched for 3-4 days. During the incubation period, the water temperature is 24-26 ℃, the water change amount is 300% -400%, the air is continuously inflated, and the seedlings are incubated for 10-12 days in succession.
(2) Fry rearing
And transferring the fertilized eggs into a seedling cultivation pond for continuous incubation 1-2 days before the seedlings are incubated. After the larvae are hatched out, the larvae enter a fry rearing pond through a pot sieving hole, or are scooped out one by water, and are transferred into the fry rearing pond for rearing after counting. The early stage is cultivated by a round water tank or a small round cement pond with the area of 1m3The height is 1.2m, and the cultivation density is 800-1000 pieces/m3(ii) a In the early stage (1-6 days), adding 10cm of fresh water every day, increasing the water level from 40cm to 1m, and starting to change water after 7 days, wherein the water exchange amount is gradually increased from 40% to 200%, and meanwhile, the nano air pipe is adopted to oxygenate, and the aeration amount is slightly small. After 30 days of age, transferring the seedlings to the area of 10m3The water exchange amount of the culture pond is increased to more than 300%, and meanwhile, the aeration amount can be slightly larger. After the young cuttlefish is hatched, feeding the eggs of the mackerel which are to be hatched to the membrane at one time, wherein the density of the eggs is 8-10 g/m2Or directly feeding blue stone spot rudimental fry after the young cuttlefish is hatched for 1d, wherein the density is 3-5 fish/ml; 10E up to tenFeeding artemia nauplii or cladocerans at the age of 25 days, wherein the density is 2-3 per ml; feeding mythic fungus or penaeus vannamei boone after 20 days of age, wherein the density is 1-2/ml, the mackerel roe is fed once, and the rest baits are fed 2 times per day. Simultaneously, EM bacteria (10 ml/m) are uniformly sprayed in the seedling pool every day3) And artemia salina prawn slices (washed by a 300-mesh net bag when in use, the concentration is 5-10 g/m3) To improve water quality and water color. The cultivation water is used after precipitation and sand filtration, the water temperature is 22-26 ℃, the daily temperature difference is less than 1 ℃, the pH is 7.8-8.2, the salinity is 28-29, and the dissolved oxygen is more than 6 mg/L. The carcass length can reach 2cm after about 50-60 days of age, and the method can be used for propagation and release and industrialized popularization and cultivation.
In the embodiment, 820 parents of the sepia dobbica are collected, the survival rate is 85 percent, 510 sexually mature sepia dobby parents are obtained, 1200 oothecas are collected, the egg laying amount is about 12.2 ten thousand, the hatchability is 90 percent, the fry breeding survival rate is 62 percent, and 6.6 thousand fries with the carcass length of about 2cm are bred.
Claims (5)
1. A method for cultivating the fry of Sepiella maindroni comprises the following steps
1) Obtaining fertilized eggs of the cuttlefish in the Duchenne squid, putting the fertilized eggs into a sieve basin in an incubation pool for incubation, and disinfecting the fertilized eggs for 1-2 min by using 20-30 ppm iodophor solution every 2-3 days before the fries are incubated out; during the incubation period, the water temperature is 20-26 ℃, the water change amount is 300-400%, the air is continuously inflated, and the seedlings are incubated for 10-12 days in sequence;
2) transferring the fertilized eggs into a seedling cultivation pond for continuous incubation 1-2 days before the seedlings are incubated; after the larvae are hatched, the larvae enter a fry rearing pond through a pot sieving hole, or are scooped out one by using water, the larvae are counted and transferred into a round fry rearing pond for rearing, and the area of the larvae is 1-6 m3The height is 1.2-1.5 m, and the cultivation density is 800-1000 pieces/m3(ii) a Adding fresh water 10cm every day 1-6 days after the fertilized eggs are hatched, increasing the water level from 40cm to 1m, changing water from 7 days old, gradually increasing the water exchange amount from 40% to 200%, and simultaneously adopting a nano air pipe to oxygenate and slightly inflate; after 30 days of age, transferring the seedlings into 10-20 m3The water exchange amount is increased to more than 300 percent by cultivating in a circular cultivating pool and cultivating in long flowing water, andincreasing the inflation quantity; precipitating and sand-filtering the cultivation water, using the water with the water temperature of 20-26 ℃, the daily temperature difference of less than 1 ℃, the pH of 7.8-8.2, the salinity of 28-32, the dissolved oxygen of more than 6mg/L, the growth of 60-70 days, and the trunk length of 2-2.5 cm, and then, sprouting;
after the young cuttlefishes are hatched successively, feeding the eggs of the mackerel with the embryo body lower bag 4/5 in a young cuttlefish breeding pond at one time, wherein the density is 8-10 g/m2Or directly feeding blue stone spot rudimental fry after the young cuttlefish is hatched for 1d, wherein the density is 3-5 fish/ml; feeding artemia nauplii or cladocerans with the density of 2-3 per ml at the age of 10-25 days; feeding the young shrimps or the penaeus vannamei boone after 20 days of age, wherein the density is 1-2/ml; feeding the mackerel roe once, and feeding the rest baits 2 times a day; EM bacteria and artemia prawn slices are uniformly sprayed in the offspring seed culture pond every day, the water color is adjusted to be tawny, the bottom is not sucked, and the stress is further reduced.
2. The method for cultivating sepia doodii offspring seeds according to claim 1, wherein the mackerel eggs are obtained by natural spawning, and the newly hatched sepia doodii are hatched in high density by adopting a hatching barrel or a hatching net cage, wherein the hatching density is 800-1000 g/m3Before feeding the cuttlefish, fishing out the cuttlefish by using a mesh bag.
3. The method for cultivating the Sepiella donnaeus seeds of claim 1, wherein the 30-day-old Sepiella donnaeus seeds are transferred into the Sepiella donnaeus seeds of 10-20 m3When the cuttlefish seeds are poured into the culture pond, the water level is slowly reduced, then the cuttlefish larvae are driven into the washbasin by the diddle net, and the whole process is operated with water, so that the cuttlefish seeds are prevented from leaving water and jetting ink.
4. The method for cultivating sepia dobby fry according to claim 1, wherein the concentration of EM bacteria is 8-10 ml/m3(ii) a When the artemia salina prawn slices are used, the artemia salina prawn slices are scrubbed by a 300-mesh net bag, and the concentration is 5-10 g/m3
5. The method for cultivating the sepia doodii offspring seeds according to claim 1, wherein the fertilized eggs of the sepia doodii are derived from the artificial cultivation of the parents, and the cultivation method comprises the following steps:
(1) harvesting of parents
Fishing the adult squid of the Dolichi gun in 7-9 months every year, and transferring the captured adult squid into a net cage or a cabin for temporary culture;
(2) transportation of parents
Transporting the captured parent to a wharf, weighing the parent with water, transferring the parent to a live fish transport vehicle, filling pure oxygen into the water vehicle by adopting nano air stones, adding 20-30 ppm of florfenicol and 8-10 ppm of aquatic product excitant into the transport water to reduce the occurrence of ink jet behaviors and prevent bacterial infection, wherein the transport density is 8-10 kg/m3The transportation time is not more than 1h, and the operation with water is carried out during loading and unloading so as to reduce the operation damage;
(3) temporary raising of parents
A circle of nano-air pipes are arranged at the bottom of the inner wall of the culture pond, an air curtain is formed during air inflation, the parents can be effectively prevented from colliding with the pond wall, air inflation and oxygenation are continuously performed, and the illumination intensity is controlled to be 400 lx-800 lx; the water temperature is 20-28 ℃, and the salinity is 25-32; a density of 5 to 8kg/m3(ii) a The running water cultivation is adopted, the water changing rate is 400-500%, and the interior of a workshop needs to be kept quiet; fresh and live baits are adopted for nutrient domestication, the daily feeding amount is 2% -3% of the weight of the cuttlefish, the cuttlefish is fed for 2 times every day, the residual baits and excrement are removed in the morning every day, and water is thoroughly changed; 3d after entering the pool, 10-20 ppm of florfenicol medicated bath is used for 1-2 h every day;
(4) collection of fertilized egg
The method is characterized in that a plastic plate with holes is adopted, a central tube of a culture pond is used as a center, the plastic plate is enclosed into a cylinder shape, the diameter of the plastic plate ranges from 60cm to 100cm, the height of the plastic plate is 10cm to 20cm higher than that of the central tube, the bottom of the culture pond is fixed, the hole diameter of the plastic plate ranges from 1cm to 2cm, the hole distance ranges from 2mm to 3mm, collected ootheca is picked off from the plastic plate with holes every day after a parent lays eggs, and the plastic plate with holes is placed into hatching equipment for hatching.
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