CN113597985A - Tripterygium wilfordii hook seed seedling raising method - Google Patents

Tripterygium wilfordii hook seed seedling raising method Download PDF

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Publication number
CN113597985A
CN113597985A CN202110896150.5A CN202110896150A CN113597985A CN 113597985 A CN113597985 A CN 113597985A CN 202110896150 A CN202110896150 A CN 202110896150A CN 113597985 A CN113597985 A CN 113597985A
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seeds
tripterygium wilfordii
seedlings
field
transplanted
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CN202110896150.5A
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Chinese (zh)
Inventor
郭盛合
刘三波
龚达林
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China Resources Sanjiu Huangshi Pharmaceutical Co ltd
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China Resources Sanjiu Huangshi Pharmaceutical Co ltd
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/28Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Inorganic Chemistry (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention relates to the technical field of seed seedling culture, in particular to a method for culturing tripterygium wilfordii seeds, which comprises the following steps; step 1: selecting healthy tripterygium wilfordii stock plant without plant diseases and insect pests in 11-12 months of each year, collecting tripterygium wilfordii fruits, sun-drying, removing peel, winnowing and removing impurities to obtain pure seeds; step 2: sand storage is carried out on the seeds in the step 1 outdoors, the seeds are taken out and soaked in water, the seeds are taken out and drained, and sandy loam or light loam with good water permeability and air permeability is selected as a seeding substrate; and step 3: the seeds germinate after sowing, and the seedlings can be transplanted to a field for planting after the height of the seedlings reaches 20 cm; and 4, step 4: before transplanting seedlings, the field needs to be subjected to leveling fertilization and then transplanted. The invention utilizes the germination characteristics of the tripterygium wilfordii seeds to carry out the tripterygium wilfordii seed seedling culture, and the seed seedlings combine the advantages of both male parent and female parent of plants, thereby having the advantages of strong stress resistance, robust root growth, high root biomass and the like, and ensuring the survival rate of the seed seedling culture.

Description

Tripterygium wilfordii hook seed seedling raising method
Technical Field
The invention relates to the technical field of seed seedling culture, in particular to a method for culturing tripterygium wilfordii seeds.
Background
Tripterygium wilfordii is a common wind-damp-dispelling medicinal material for plants in the genus of Tripterygium in the family of Celastraceae, and as the application of Tripterygium wilfordii is continuously deepened, the wild resources cannot meet the market demand, and the artificial cultivation is in the forefront; since artificial cultivation of tripterygium wilfordii has started in recent years, various researches are relatively weak, seedling breeding researches which are the most important of planting work are very good, most of the researches are concentrated on the aspect of cutting seedling, and the research on seed seedling is relatively less;
at present, the most widely used method for raising the seedlings of the thunder god vine is cutting propagation, but the cutting seedlings have the defects of poor stress resistance, relatively slow root growth and even incapability of rooting in the growth process, and the like, thereby seriously restricting the development of the thunder god vine planting work.
Disclosure of Invention
The invention aims to solve the defect that the root of a cutting seedling grows slowly in the prior art, and provides a method for raising the seedlings of tripterygium wilfordii seeds.
In order to achieve the purpose, the invention adopts the following technical scheme:
designing a method for raising seedlings of tripterygium wilfordii seeds, comprising the following steps;
step 1: selecting healthy tripterygium wilfordii stock plant without plant diseases and insect pests to grow in 11-12 months of each year, harvesting tripterygium wilfordii fruits, then placing the tripterygium wilfordii stock plant in a place with sufficient sunlight for drying for 2-3 days, removing peel after drying in the sun, winnowing and removing impurities to obtain pure seeds;
step 2: the seeds in the step 1 are stored in sand for 2-3 months outdoors, namely taken out for seeding in the second 2-3 months, taken out and soaked in water at the temperature of 20-25 ℃ for 12h, taken out and drained, and sandy loam or light loam with good water permeability and air permeability is selected as a seeding substrate;
and step 3: after sowing, keeping the air humidity between 60% and 70%, keeping the temperature between 25 ℃ and 30 ℃, germinating 1 month later, spraying 0.01% urea solution after the seedling of the seed reaches 5cm, continuously spraying for 3 times at intervals of 15 days every time, and transplanting the seedling to a field for planting after the seedling reaches 20 cm;
and 4, step 4: before the seedlings are transplanted, the field needs to be subjected to flat fertilization, the row spacing of the transplanted seedlings is 100cm multiplied by 100cm, the planting density is kept at 9000-; among them, timely weeding, scientific topdressing and reasonable irrigation are required.
Preferably, in step 1, the harvested tripterygium wilfordii fruits need to be winged, so that the quality of the seeds can be prevented from being influenced by oxidative necrosis of the tripterygium wilfordii fruits in the transportation process.
Preferably, in step 2, the required relative humidity of the sowing soil is kept between 60% and 70%, the row spacing of the sowing plants is 5cm multiplied by 10cm, fine sand with the thickness of 3cm is covered after sowing, and the seeds are watered thoroughly, so that the normal growth of the seeds can be ensured.
Preferably, in the step 4, the process of leveling the transplanted field comprises the following steps;
firstly, loosening and irrigating the transplanted field to keep the soil moist, and removing surface weeds;
then, adding fertilizer into the transplanted field, deeply ploughing the soil 3 days after fertilizing, leveling and thinning, and hilling and compacting around the bed;
and finally, spreading nutrient soil with the thickness of 3-4cm on the bed surface, and transplanting the seedlings after leveling.
Preferably, the nutrient soil comprises leaf mold, plant ash, wormcast, diatomite, mushroom dregs, decomposed farmyard manure, polyglutamic acid, trace element fertilizer and aspirin, so that sufficient nutrient components required by seedling growth can be ensured in the soil transplanted to the field.
The method for raising the seedlings of the tripterygium wilfordii seeds has the beneficial effects that: the invention utilizes the germination characteristics of the tripterygium wilfordii seeds to carry out the tripterygium wilfordii seed seedling culture, and the seed seedlings combine the advantages of both male parent and female parent of plants, thereby having the advantages of strong stress resistance, robust root growth, high root biomass and the like, and ensuring the survival rate of the seed seedling culture.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments.
Example 1:
a method for raising seedling of Tripterygium wilfordii hook F seed comprises the following steps;
step 1: selecting healthy tripterygium wilfordii parent plant without plant diseases and insect pests to grow in 11-12 months of each year to harvest tripterygium wilfordii fruits, wherein the harvested tripterygium wilfordii fruits need to be winged, so that the quality of seeds can be prevented from being influenced by oxidative necrosis of the tripterygium wilfordii fruits in the process of transportation; then placing the seeds in a place with sufficient sunlight for drying for 2 days, removing peels after drying in the sun, and winnowing to remove impurities to obtain pure seeds;
step 2: the seeds in the step 1 are stored in sand for 3 months outdoors, namely, the seeds are taken out for sowing in 2-3 months of the next year, the seeds are taken out and soaked in water at the temperature of 20 ℃ for 12 hours, the seeds are taken out and drained, sandy loam or light loam with good water permeability and air permeability is selected as a sowing substrate, the required relative humidity of the sowing soil is kept between 70 percent, the row spacing of sowing plants is 5cm multiplied by 10cm, fine sand with the thickness of 3cm is covered after sowing, and water is poured thoroughly, so that the normal growth of the seeds can be ensured;
and step 3: after sowing, keeping the air humidity between 60 percent and the temperature between 30 ℃, germinating the seeds after 1 month, spraying 0.01 percent urea solution after the seedlings reach 5cm, continuously spraying for 3 times at intervals of 15 days every time, and transplanting the seedlings to a field for planting after the seedlings reach 20 cm;
and 4, step 4: before transplanting seedlings, the field needs to be subjected to leveling fertilization, and the method comprises the following steps;
firstly, loosening and irrigating the transplanted field to keep the soil moist, and removing surface weeds; then, adding fertilizer into the transplanted field, deeply ploughing the soil 3 days after fertilizing, leveling and thinning, and hilling and compacting around the bed; finally, laying nutrient soil with the thickness of 3cm on the bed surface, and transplanting seedlings after leveling; the nutrient soil comprises leaf mold, plant ash, wormcast, diatomite, mushroom dregs, decomposed farmyard manure, polyglutamic acid, trace element fertilizer and aspirin, so that sufficient nutrient components required by seedling growth can be ensured in the soil transplanted to the field;
the row spacing of transplanted plants is 100cm multiplied by 100cm, the planting density is kept at 9000 plants per hectare, the survival rate needs to be checked in time after the plants are transplanted to a field, and the survival rate is ensured when the plants are found to be replanted in time; among them, timely weeding, scientific topdressing and reasonable irrigation are required.
The invention utilizes the germination characteristics of the tripterygium wilfordii seeds to carry out the tripterygium wilfordii seed seedling culture, and the seed seedlings combine the advantages of both male parent and female parent of plants, thereby having the advantages of strong stress resistance, robust root growth, high root biomass and the like, and ensuring the survival rate of the seed seedling culture.
Example 2:
a method for raising seedling of Tripterygium wilfordii hook F seed comprises the following steps;
step 1: selecting healthy tripterygium wilfordii parent plant without plant diseases and insect pests to grow in 11-12 months of each year to harvest tripterygium wilfordii fruits, wherein the harvested tripterygium wilfordii fruits need to be winged, so that the quality of seeds can be prevented from being influenced by oxidative necrosis of the tripterygium wilfordii fruits in the process of transportation; then placing the seeds in a place with sufficient sunlight for 3 days, removing peels after drying in the sun, and winnowing to remove impurities to obtain pure seeds;
step 2: the seeds in the step 1 are stored in sand outdoors for 2 months, namely, the seeds are taken out for sowing in 2-3 months of the next year, the seeds are taken out and soaked in water at 22 ℃ for 12 hours, the seeds are taken out and drained, sandy loam or light loam with good water permeability and air permeability is selected as a sowing substrate, the required relative humidity of the sowing soil is kept between 65 percent, the row spacing of sowing plants is 5cm multiplied by 10cm, fine sand with the thickness of 3cm is covered after sowing, and water is poured thoroughly, so that the normal growth of the seeds can be ensured;
and step 3: after sowing, keeping the air humidity between 65 percent and the temperature between 27 ℃, germinating the seeds after 1 month, spraying 0.01 percent urea solution after the seedlings reach 5cm, continuously spraying for 3 times at intervals of 15 days every time, and transplanting the seedlings to a field for planting after the seedlings reach 20 cm;
and 4, step 4: before transplanting seedlings, the field needs to be subjected to leveling fertilization, and the method comprises the following steps;
firstly, loosening and irrigating the transplanted field to keep the soil moist, and removing surface weeds; then, adding fertilizer into the transplanted field, deeply ploughing the soil 3 days after fertilizing, leveling and thinning, and hilling and compacting around the bed; finally, laying nutrient soil with the thickness of 3.5cm on the bed surface, and transplanting seedlings after leveling; the nutrient soil comprises leaf mold, plant ash, wormcast, diatomite, mushroom dregs, decomposed farmyard manure, polyglutamic acid, trace element fertilizer and aspirin, so that sufficient nutrient components required by seedling growth can be ensured in the soil transplanted to the field;
the row spacing of transplanted plants is 100cm multiplied by 100cm, the planting density is kept at 9500 plants per hectare, the survival rate needs to be checked in time after the plants are transplanted to a field, and the survival rate is ensured when the plants are found to be replanted in time; among them, timely weeding, scientific topdressing and reasonable irrigation are required.
Example 3:
a method for raising seedling of Tripterygium wilfordii hook F seed comprises the following steps;
step 1: selecting healthy tripterygium wilfordii parent plant without plant diseases and insect pests to grow in 11-12 months of each year to harvest tripterygium wilfordii fruits, wherein the harvested tripterygium wilfordii fruits need to be winged, so that the quality of seeds can be prevented from being influenced by oxidative necrosis of the tripterygium wilfordii fruits in the process of transportation; then placing the seeds in a place with sufficient sunlight for 3 days, removing peels after drying in the sun, and winnowing to remove impurities to obtain pure seeds;
step 2: the seeds in the step 1 are stored in sand outdoors for 2 months, namely, the seeds are taken out for sowing in 2-3 months of the next year, the seeds are taken out and soaked in water at 25 ℃ for 12 hours, the seeds are taken out and drained, sandy loam or light loam with good water permeability and air permeability is selected as a sowing substrate, the required relative humidity of the sowing soil is kept between 60 percent, the row spacing of sowing plants is 5cm multiplied by 10cm, fine sand with the thickness of 3cm is covered after sowing, and water is poured thoroughly, so that the normal growth of the seeds can be ensured;
and step 3: after sowing, keeping the air humidity between 70% and the temperature between 25 ℃, germinating 1 month later, spraying 0.01% urea solution after the seedling reaches 5cm, continuously spraying for 3 times at intervals of 15 days each time, and transplanting the seedling to a field for planting after the seedling reaches 20 cm;
and 4, step 4: before transplanting seedlings, the field needs to be subjected to leveling fertilization, and the method comprises the following steps;
firstly, loosening and irrigating the transplanted field to keep the soil moist, and removing surface weeds; then, adding fertilizer into the transplanted field, deeply ploughing the soil 3 days after fertilizing, leveling and thinning, and hilling and compacting around the bed; finally, laying nutrient soil with the thickness of 4cm on the bed surface, and transplanting seedlings after leveling; the nutrient soil comprises leaf mold, plant ash, wormcast, diatomite, mushroom dregs, decomposed farmyard manure, polyglutamic acid, trace element fertilizer and aspirin, so that sufficient nutrient components required by seedling growth can be ensured in the soil transplanted to the field;
the row spacing of transplanted plants is 100cm multiplied by 100cm, the planting density is kept at 10000 plants per hectare, the survival rate needs to be checked in time after the plants are transplanted to a field, and the survival rate is guaranteed when the plants are found to be replanted in time; among them, timely weeding, scientific topdressing and reasonable irrigation are required.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.

Claims (5)

1. A method for raising seedlings of tripterygium wilfordii seeds is characterized by comprising the following steps;
step 1: selecting healthy tripterygium wilfordii stock plant without plant diseases and insect pests to grow in 11-12 months of each year, harvesting tripterygium wilfordii fruits, then placing the tripterygium wilfordii stock plant in a place with sufficient sunlight for drying for 2-3 days, removing peel after drying in the sun, winnowing and removing impurities to obtain pure seeds;
step 2: the seeds in the step 1 are stored in sand for 2-3 months outdoors, namely taken out for seeding in the second 2-3 months, taken out and soaked in water at the temperature of 20-25 ℃ for 12h, taken out and drained, and sandy loam or light loam with good water permeability and air permeability is selected as a seeding substrate;
and step 3: after sowing, keeping the air humidity between 60% and 70%, keeping the temperature between 25 ℃ and 30 ℃, germinating 1 month later, spraying 0.01% urea solution after the seedling of the seed reaches 5cm, continuously spraying for 3 times at intervals of 15 days every time, and transplanting the seedling to a field for planting after the seedling reaches 20 cm;
and 4, step 4: before the seedlings are transplanted, the field needs to be subjected to flat fertilization, the row spacing of the transplanted seedlings is 100cm multiplied by 100cm, the planting density is kept at 9000-; among them, timely weeding, scientific topdressing and reasonable irrigation are required.
2. A method for raising seedlings of Tripterygium wilfordii seeds according to claim 1, wherein in step 1, the harvested Tripterygium wilfordii fruits need to have wings to avoid the oxidation necrosis of the Tripterygium wilfordii fruits during transportation back to affect the quality of the seeds.
3. A method for raising seedlings of tripterygium wilfordii seeds according to claim 1, wherein in step 2, the relative humidity required for sowing soil is kept between 60% and 70%, the row spacing of sowing plants is 5cm x 10cm, fine sand with the thickness of 3cm is covered after sowing, and the seeds are watered thoroughly to ensure the normal growth of the seeds.
4. A method for raising seedlings of Tripterygium wilfordii hook F seeds according to claim 1, wherein in the step 4, the step of flattening the field for transplanting comprises the following steps;
firstly, loosening and irrigating the transplanted field to keep the soil moist, and removing surface weeds;
then, adding fertilizer into the transplanted field, deeply ploughing the soil 3 days after fertilizing, leveling and thinning, and hilling and compacting around the bed;
and finally, spreading nutrient soil with the thickness of 3-4cm on the bed surface, and transplanting the seedlings after leveling.
5. A method for raising seedlings of tripterygium wilfordii seeds as claimed in claim 4, wherein the nutrient soil is composed of leaf mold, plant ash, wormcast, diatomite, mushroom dregs, decomposed farmyard manure, polyglutamic acid, trace element fertilizer and aspirin.
CN202110896150.5A 2021-08-05 2021-08-05 Tripterygium wilfordii hook seed seedling raising method Pending CN113597985A (en)

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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2352179A1 (en) * 1998-11-23 2000-06-02 The United States Of America, As Represented By The Secretary Of Agriculture Thlaspi caerulescens subspecies for cadmium and zinc recovery
CN1994044A (en) * 2006-12-21 2007-07-11 浙江省中药研究所有限公司 Method for planting Chinese herb triperygium wilfordii
CN102106231A (en) * 2010-12-01 2011-06-29 福建省汉堂生物制药股份有限公司 Tripterygium wilfordii hook acclimatization and high-yielding method
CN108849301A (en) * 2018-07-26 2018-11-23 安龙县欣蔓生物科技有限责任公司 A kind of cultural method of tripterygium wilfordii
CN113141888A (en) * 2020-01-07 2021-07-23 云南罗瑞生物科技有限公司 Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2352179A1 (en) * 1998-11-23 2000-06-02 The United States Of America, As Represented By The Secretary Of Agriculture Thlaspi caerulescens subspecies for cadmium and zinc recovery
CN1994044A (en) * 2006-12-21 2007-07-11 浙江省中药研究所有限公司 Method for planting Chinese herb triperygium wilfordii
CN102106231A (en) * 2010-12-01 2011-06-29 福建省汉堂生物制药股份有限公司 Tripterygium wilfordii hook acclimatization and high-yielding method
CN108849301A (en) * 2018-07-26 2018-11-23 安龙县欣蔓生物科技有限责任公司 A kind of cultural method of tripterygium wilfordii
CN113141888A (en) * 2020-01-07 2021-07-23 云南罗瑞生物科技有限公司 Quick cutting propagation and seedling raising technology for medicinal vine tripterygium wilfordii twigs

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
刘三波 等: "雷公藤种子发芽特性研究", 湖北农业科学, no. 2, pages 143 - 145 *

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