CN113597900A - Miniature Chinese rose cuttage efficient rooting method - Google Patents
Miniature Chinese rose cuttage efficient rooting method Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/28—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing peat, moss or sphagnum
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
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Abstract
The invention discloses a miniature Chinese rose cutting efficient rooting method, which comprises the following steps: s1: selecting annual and biennial miniature Chinese rose branches to trim, trimming into 5-8cm cuttings, at least reserving 2 axillary buds and 1-2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5-1.0cm, trimming into a flat port, and the distance between the lower port of each cutting and the axillary bud is 0.6-0.8cm, and trimming into an oblique port; s2: refrigerating the cuttings obtained by the trimming of the step S1; s3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking; s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4-5 weeks to obtain a miniature Chinese rose cutting rooting seedling; s5: transplanting the miniature Chinese rose cutting rooted seedlings obtained in the step S4, wherein the matrix components adopted by transplanting comprise peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the cutting propagation method for obtaining the miniature Chinese rose cutting seedlings has the advantages of short time, high survival rate, low cost and energy consumption, easiness in operation, regular flowering, capability of realizing the efficient large-scale production of the miniature Chinese rose and wide application prospect.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a micro-China rose cuttage efficient rooting method.
Background
Miniature Chinese Roses (Miniature Roses) are evergreen and short shrubs of Rosa (Rosaceae) genus (Rosa). The flower fragrance is unique, the flower color is fantastic, the flower shape is elegant, the medicinal effect can be realized, and the ornamental value and the economic value are very unique. Flowers are clustered or singly grown, the florescence is continuous, the cultivation is easy, the adaptability is strong, and the method is a popular flower germplasm resource for household gardening and gardens. Due to the gradual increase of the market demand and the use amount of the miniature Chinese roses, the cultivation and breeding technology of modern miniature Chinese roses is gradually improved.
However, the traditional simple cutting method has the defects of poor cutting quality, low cutting rooting rate and the like. In order to improve the rooting rate, the survival rate and the quality of transplanted flowers of the miniature Chinese rose cutting, researchers at home and abroad carry out a large number of miniature Chinese rose cutting breeding experiments. In addition to the plant physiological properties of miniature chinese rose varieties, numerous research results indicate: the main factors influencing the cuttage of the miniature Chinese roses comprise cuttage substrates, growth climate conditions, cutting positions of the cutting slips, the types and the concentrations of plant growth regulators, the processing time, rhizosphere germs and the like. Although the survival rate of the cutting production of the miniature Chinese rose is greatly improved compared with the survival rate of the cutting production of the miniature Chinese rose in the past, in the factory seedling culture of the miniature Chinese rose, although a plurality of enterprises construct mature industrial processes and systems, the cutting rooting agent still depends on foreign import, and the obtained theoretical reserve is not timely applied to the practice of cutting propagation of the miniature Chinese rose, and has a certain difference with the international cutting level. Therefore, it is urgently needed to develop a micro-China rose cutting efficient rooting method to solve the technical problems.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a miniature Chinese rose cutting efficient rooting method, which is short in time required for obtaining miniature Chinese rose cutting seedlings through cutting propagation, high in survival rate, low in cost and energy consumption, easy to operate, regular in flowering, capable of realizing miniature Chinese rose efficient large-scale production, wide in application prospect and beneficial to popularization and application.
In order to achieve the purpose, the invention provides a miniature Chinese rose cutting efficient rooting method, which comprises the following steps:
s1: selecting annual and biennial miniature Chinese rose branches to trim, trimming into 5-8cm cuttings, at least reserving 2 axillary buds and 1-2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5-1.0cm, trimming into a flat port, and the distance between the lower port of each cutting and the axillary bud is 0.6-0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1;
s3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4-5 weeks to obtain a miniature Chinese rose cutting rooting seedling;
s5: transplanting the miniature Chinese rose cutting rooted seedlings obtained in the step S4, wherein the matrix components adopted by transplanting comprise peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3.
Preferably, in S1, the standard for selecting the miniature chinese rose branches is: selecting branches at the middle and lower parts of the first or second year, wherein the branches are healthy and strong and have no diseases and insect pests, no mechanical damage and plump axillary buds but do not sprout or flower branches 1-2 weeks after flower falling as the branches.
Preferably, in S2, the refrigeration time of the cuttings is 2-5 days, and the refrigeration temperature is 3-5 ℃.
Preferably, in the S3, the soaking time of the cutting in the naphthylacetic acid solution is 15-30 min.
Preferably, in the S3, the concentration of the naphthylacetic acid solution is 25-50 mg/L.
Preferably, in S4, the specification of the 128-hole plug is 3cm × 1.5cm × 3.8cm, and one cutting strip is inserted into each hole.
Preferably, in the step S4, the matrix component in the 128-hole plug comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 8: 2.
Preferably, in the step S4, the cutting slip is inserted into the 128-hole plug to a depth of 1/2-2/3 of the plug.
Preferably, in the S4, the culture temperature is 18-30 ℃.
Preferably, in the S5, the culture temperature is 15-28 ℃.
The miniature Chinese rose cuttage efficient rooting method provided by the invention has the following beneficial effects.
1. The method for obtaining the miniature Chinese rose cutting seedlings through cutting propagation has the advantages of short time, high survival rate, low cost and energy consumption, regular flowering, capability of realizing high-efficiency large-scale production of the miniature Chinese rose, wide application prospect and contribution to popularization and application.
2. Compared with the traditional cuttage method adopting substrates such as garden soil, yellow core soil, sand bed and the like, the cuttage method disclosed by the invention is based on a 128-hole plug, and then the substrates are filled, wherein the substrates adopt peat soil and perlite, and the volume ratio of the peat soil to the perlite is 8: 2, the cutting slips and the matrix can be tightly combined together, the bottom of the matrix can be kept moist for a long time, the infection of harmful germs can be prevented, and the cutting failure caused by the germ infection or water shortage of the cutting slips can be avoided.
3. The transplanting process of the plug seedlings does not damage root systems, the survival rate is high after the planting, the seedling revival is not needed, the seedlings are more suitable for long-distance transportation, the commercialized supply is convenient, and the like.
4. The invention can refrigerate the cuttings, effectively improve the rooting rate and survival rate of the cuttings, and can obviously improve the flowering uniformity and the flower number after the pots are changed and transplanted.
5. The cutting is soaked in the naphthylacetic acid solution with a certain concentration for a certain time, so that a considerable amount of exogenous hormones are accumulated, the rooting rate and the survival rate of the cutting are promoted, and the survival rate of transplanting is improved.
Detailed Description
The present invention will be further described with reference to specific examples to assist understanding of the invention.
The invention provides a miniature Chinese rose cuttage efficient rooting method, which comprises the following steps:
s1: selecting annual and biennial miniature Chinese rose branches to trim, trimming into 5-8cm cuttings, at least reserving 2 axillary buds and 1-2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5-1.0cm, trimming into a flat port, and the distance between the lower port of each cutting and the axillary bud is 0.6-0.8cm, and trimming into an oblique port; the standard for selecting the miniature Chinese rose branches is as follows: selecting branches at the middle and lower parts of the first or second year, wherein the branches are healthy and strong and have no diseases and insect pests, no mechanical damage and plump axillary buds but do not sprout or flower branches 1-2 weeks after flower falling as the branches. If the leaves of the cutting to be trimmed are larger, 1/2 leaf areas of 1-2 leaves are preferentially reserved, so that water and nutrients in the cutting can be effectively prevented from losing, and rooting of the cutting and growth control of branches and leaves are facilitated. In the invention, the plain end of the upper port of the cutting is not easy to lose more water, thus being beneficial to survival; the lower end opening of the cutting is cut into an oblique opening, the oblique opening is preferably kept smooth, the cutting resistance is reduced, the callus is favorably formed, and the rooting rate and the survival rate of the miniature Chinese rose cutting are improved.
S2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 2-5 days, and the cold storage temperature is 3-5 ℃.
S3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking; soaking the cutting in naphthylacetic acid solution for 15-30 min. The concentration of the naphthylacetic acid solution is 25-50 mg/L. The naphthylacetic acid is used for promoting the cutting to take root, and the external hormone is added to ensure that the base part of the cutting absorbs the rooting hormone, thereby promoting the taking root, shortening the rooting time and improving the rooting rate and the survival rate of the miniature Chinese rose cutting.
S4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4-5 weeks at the culture temperature of 18-30 ℃ to obtain miniature Chinese rose cutting rooting seedlings which can be subjected to cutting all year round; the specification of the 128-hole plug tray is 3cm multiplied by 1.5cm multiplied by 3.8cm, and one cutting strip is inserted in each hole. The matrix components in the 128-hole plug comprise peat soil and perlite, and the volume ratio of the peat soil to the perlite is 8: 2. the depth of the cutting inserted into the 128-hole plug tray is 1/2-2/3 of the plug tray. More preferably 2/3, the depth of the plug is more favorable for moisture preservation, callus formation and rooting. When cutting is carried out, the leaves are preferably kept to be consistent in orientation, the leaves are not mutually shielded, the illumination is sufficient, and the transportation and the metabolism of nutrients and moisture are facilitated. The culture temperature is more preferably 20 to 25 ℃. The invention preferably uses the sunshade net in autumn when the temperature difference is obvious and the sunshine is strong in noon and in noon. The invention preferably adopts a ventilation state, scatters light or builds a sunshade net with 60-70% sunshade rate, waters 3 times a day before rooting, and adopts the intelligent ventilation and illumination condition of the glass greenhouse. In the invention, the root starts to grow after 2 weeks of cuttage, the root system grows well after 4-5 weeks, and the rooting rate reaches more than 93%.
S5: transplanting the miniature Chinese rose cutting rooted seedlings obtained in the step S4, wherein the matrix components adopted by transplanting comprise peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3. the culture temperature is 15-28 ℃. The mixed matrix is loose and breathable, has good drainage, can provide a superior growing environment for the root system, has a proper air/water ratio, and is beneficial to fully absorbing nutrients. The source of the peat soil and perlite in the present invention is not particularly limited, and conventional commercial products of imported peat soil and perlite well known to those skilled in the art may be used. The survival rate after transplanting is more than 90%.
Example 1
S1: selecting and pruning 5 first-second-generation miniature Chinese rose branches of different varieties into 5 cm-long cutting slips, wherein each cutting slip at least reserves 2 axillary buds and 2 top leaves, the distance between the upper port of the cutting slip and the upper axillary bud is 0.5m, the cutting slip is cut into a flat port, the distance between the lower port of the cutting slip and the axillary bud is 0.8cm, and the cutting slip is cut into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking, wherein the concentration of the naphthylacetic acid is 25mg/L, and the soaking time is 15 min;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4 weeks at the culture temperature of 25 ℃ to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after culturing for 4 weeks, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
After 4 weeks of culture, the rooting rate is 93%, the cutting has 3 roots, the roots of the cutting grow out, and a small amount of the roots penetrate out of the bottom of the plug tray.
Example 2
S1: selecting 5 annual and biennial miniature Chinese rose branches of different varieties to trim, trimming into 8cm cuttings, wherein each cutting at least retains 2 axillary buds and 1 top leaf, the upper port of each cutting is 1.0cm away from the upper axillary bud, and is trimmed into a flat port, and the lower port of each cutting is 0.6cm away from the axillary bud, and is trimmed into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: and (3) soaking the refrigerated cutting of S2 in a naphthylacetic acid solution, wherein the concentration of the naphthylacetic acid is 25mg/L, and the soaking time is 30 min:
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 5 weeks at the culture temperature of 25 ℃ to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after 5 weeks of culture, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
After 5 weeks of culture, the rooting rate is 96%, the cutting has 3 roots, the roots of the cutting grow out, and part of the roots penetrate out of the bottom of the plug tray.
Example 3
S1: selecting 5 annual and biennial miniature Chinese rose branches of different varieties to trim, trimming into 5cm cuttings, at least reserving 2 axillary buds and 2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5cm, trimming into a flat port, the distance between the lower port of each cutting and the axillary bud is 0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: soaking the refrigerated cutting of S2 in naphthylacetic acid solution at concentration of 50mg/L for 15 min;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4 weeks at the culture temperature of 25 ℃ to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after culturing for 4 weeks, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
After 4 weeks of culture, the rooting rate of the cuttings can reach more than 98%, the cuttings have 3-5 roots, and most of the cuttings penetrate out of the bottom of the plug tray.
Comparative example 1
S1: selecting 5 annual and biennial miniature Chinese rose branches of different varieties to trim, trimming into 5cm cuttings, wherein each cutting at least retains 2 axillary buds and 2 top leaves, the distance between the upper port of each cutting and the upper axillary bud is 0.5cm, trimming into a flat port, the distance between the lower port of each cutting and the axillary bud is 0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4 weeks at the culture temperature of 25 ℃, and taking fine river sand as a cutting medium in the plug tray to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after culturing for 4 weeks, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
Compared with the method in the embodiment 3, due to the poor water retention of sand, the seedlings cannot be supplemented with water in time, the water shortage is serious, the rooting time of the method is delayed by 1.5 weeks compared with that of the method in the embodiment 3, and the rooting rate of the cuttage is 40%.
Comparative example 2
S1: selecting 5 annual and biennial miniature Chinese rose branches of different varieties to trim, trimming into 5cm cuttings, wherein each cutting at least retains 2 axillary buds and 2 top leaves, the distance between the upper port of each cutting and the upper axillary bud is 0.5cm, trimming into a flat port, the distance between the lower port of each cutting and the axillary bud is 0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking, wherein the concentration of the naphthylacetic acid is 100mg/L, and the soaking time is 1 h;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4 weeks at the culture temperature of 25 ℃ to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after culturing for 4 weeks, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
The transplanting survival rate of the transplanting method is 70%, but compared with the scheme of the embodiment 3, the transplanting method is slow in late-stage growth of the nursery stock, and the experiment proves that: the rooting agent has more obvious concentration and treatment time, is more favorable for the growth of seedling roots and has better growth vigor.
Comparative example 3
S1: selecting 5 annual and biennial miniature Chinese rose branches of different varieties to trim, trimming into 5cm cuttings, at least reserving 2 axillary buds and 2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5cm, trimming into a flat port, the distance between the lower port of each cutting and the axillary bud is 0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1; the cold storage time of the cutting is 3 days, and the cold storage temperature is 4 ℃.
S3: soaking the refrigerated cuttings of the step S2 in water;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4 weeks at the culture temperature of 25 ℃ to obtain 5 miniature Chinese rose cutting rooting seedlings of different varieties;
s5: after culturing for 4 weeks, transplanting, wherein the adopted matrix for transplanting comprises peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3, the culture temperature is 20 ℃.
The transplanting survival rate of the transplanting method is 60%, but compared with the scheme of the embodiment 3, by adopting the transplanting method, the seedling grows slowly in the later period and flowers irregularly, and the experiment proves that: the rooting agent has a more remarkable using effect, is more beneficial to the growth of seedling roots and has better growth vigor.
The method for obtaining the miniature Chinese rose cutting seedlings through cutting propagation has the advantages of short time, high survival rate, low cost and energy consumption, regular flowering, capability of realizing high-efficiency large-scale production of the miniature Chinese rose, wide application prospect and contribution to popularization and application. Compared with the traditional cuttage method adopting substrates such as garden soil, yellow core soil, sand bed and the like, the cuttage method disclosed by the invention is based on a 128-hole plug, and then the substrates are filled, wherein the substrates adopt peat soil and perlite, and the volume ratio of the peat soil to the perlite is 8: 2, the cutting slips and the matrix can be tightly combined together, the bottom of the matrix can be kept moist for a long time, the infection of harmful germs can be prevented, and the cutting failure caused by the germ infection or water shortage of the cutting slips can be avoided. The transplanting process of the plug seedlings does not damage root systems, the survival rate is high after the planting, the seedling revival is not needed, the seedlings are more suitable for long-distance transportation, the commercialized supply is convenient, and the like. The invention can refrigerate the cuttings, effectively improve the rooting rate and survival rate of the cuttings, and can obviously improve the flowering uniformity and the flower number after the pots are changed and transplanted. The cutting is soaked in the naphthylacetic acid solution with a certain concentration for a certain time, so that a considerable amount of exogenous hormones are accumulated, the rooting rate and the survival rate of the cutting are promoted, and the survival rate of transplanting is improved.
The inventive concept is explained in detail herein using specific examples, which are given only to aid in understanding the core concepts of the invention. It should be understood that any obvious modifications, equivalents and other improvements made by those skilled in the art without departing from the spirit of the present invention are included in the scope of the present invention.
Claims (10)
1. A micro-China rose cuttage efficient rooting method is characterized by comprising the following steps:
s1: selecting annual and biennial miniature Chinese rose branches to trim, trimming into 5-8cm cuttings, at least reserving 2 axillary buds and 1-2 top leaves for each cutting, wherein the distance between the upper port of each cutting and the upper axillary bud is 0.5-1.0cm, trimming into a flat port, and the distance between the lower port of each cutting and the axillary bud is 0.6-0.8cm, and trimming into an oblique port;
s2: refrigerating the cuttings obtained by the trimming of the step S1;
s3: placing the refrigerated cutting of S2 into a naphthylacetic acid solution for soaking;
s4: inserting the cutting obtained in the step S3 into a 128-hole plug tray for culturing for 4-5 weeks to obtain a miniature Chinese rose cutting rooting seedling;
s5: transplanting the miniature Chinese rose cutting rooted seedlings obtained in the step S4, wherein the matrix components adopted by transplanting comprise peat soil and perlite, and the volume ratio of the peat soil to the perlite is 7: 3.
2. The micro-Chinese rose cutting efficient rooting method according to claim 1, wherein in S1, the standards for selecting micro-Chinese rose branches are as follows: selecting branches at the middle and lower parts of the first or second year, wherein the branches are healthy and strong and have no diseases and insect pests, no mechanical damage and plump axillary buds but do not sprout or flower branches 1-2 weeks after flower falling as the branches.
3. The method for rooting by cutting of miniature Chinese roses efficiently according to claim 2, wherein in S2, the cold storage time of the cuttings is 2-5 days, and the cold storage temperature is 3-5 ℃.
4. The method for rooting by micro-rose cutting with high efficiency according to claim 3, wherein in S3, the soaking time of the cutting in the naphthylacetic acid solution is 15-30 min.
5. The method for rooting by cutting of miniature Chinese roses efficiently according to claim 4, wherein in S3, the concentration of the naphthylacetic acid solution is 25-50 mg/L.
6. The method for rooting by cutting Chinese rose in high efficiency according to claim 5, wherein in S4, the specification of a 128-hole plug is 3cm x 1.5cm x 3.8cm, and one cutting slip is inserted in each hole.
7. The method for rooting by cutting of miniature Chinese roses efficiently according to claim 6, wherein in S4, the matrix components in the 128-hole plug comprise peat soil and perlite in a volume ratio of 8: 2.
8. The method for rooting by cutting of miniature Chinese roses efficiently according to claim 7, wherein in S4, the cutting slips are inserted into a 128-hole plug tray to a depth of 1/2-2/3 of the plug tray.
9. The method for efficient rooting by cutting of miniature Chinese roses according to claim 8, wherein the culture temperature in S4 is 18-30 ℃.
10. The method for efficient rooting by cutting of miniature Chinese roses according to claim 9, wherein the culture temperature in S5 is 15-28 ℃.
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| CN104365482A (en) * | 2014-11-17 | 2015-02-25 | 苏州市新巷农艺科技园 | Miniature Chinese rose tissue culture breeding method |
| CN107125120A (en) * | 2017-06-19 | 2017-09-05 | 房丹 | A kind of Chinese rose cuttage breeding method |
| CN111602525A (en) * | 2020-06-03 | 2020-09-01 | 中国科学院成都生物研究所 | Method for raising seedlings of single-petal Chinese roses through cutting in winter |
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2021
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104365482A (en) * | 2014-11-17 | 2015-02-25 | 苏州市新巷农艺科技园 | Miniature Chinese rose tissue culture breeding method |
| CN107125120A (en) * | 2017-06-19 | 2017-09-05 | 房丹 | A kind of Chinese rose cuttage breeding method |
| CN111602525A (en) * | 2020-06-03 | 2020-09-01 | 中国科学院成都生物研究所 | Method for raising seedlings of single-petal Chinese roses through cutting in winter |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114946500A (en) * | 2022-05-23 | 2022-08-30 | 罗开春 | Miniature Chinese rose cultivation method |
| CN114946500B (en) * | 2022-05-23 | 2023-06-27 | 罗开春 | Miniature Chinese rose cultivation method |
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