CN1135267C - Method for preparing soybean polypeptide by utilizing ereyme method - Google Patents
Method for preparing soybean polypeptide by utilizing ereyme method Download PDFInfo
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- CN1135267C CN1135267C CNB011428236A CN01142823A CN1135267C CN 1135267 C CN1135267 C CN 1135267C CN B011428236 A CNB011428236 A CN B011428236A CN 01142823 A CN01142823 A CN 01142823A CN 1135267 C CN1135267 C CN 1135267C
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Abstract
The present invention provides a production technique for preparing soybean polypeptide through the enzymolysis of soybean proteins using protease. Soybean concentrated proteins are mainly used as raw materials, and the soybean proteins are hydrolyzed by two kinds of protease to prepare the soybean polypeptide. Production processes comprise the pretreatment of raw material liquid, enzyme decomposition, separation through ultrafiltration, concentration and desiccation, and the technique is simple and is suitable for industrial production. Products can be directly eaten, can also be used as food nutrition enrichments to increase protein content, can also be used for acid plant protein beverages, sport health foods, military foods, etc., and the present invention opens up a new way for the deep processing of the soybean proteins.
Description
The present invention relates to a kind of production technique of method for preparing soybean polypeptide by utilizing ereyme, soybean polypeptide can be used as the nutrition-fortifying agent of nutritious prod, protective foods.
Soybean polypeptide is the hydrolysate of soy-protein after the enzyme effect, because it has good nutrition absorption characteristic and splendid physiological function and processing characteristics, is just receiving albumen producer's concern.Patent documentation once utilized the microbial process soybean protein to produce soybean polypeptide in the past, but complicated operating process, technique controlling difficulty are big, are not easy to large-scale industrial production.Therefore, general soybean processing polypeptide multiselect is produced soybean polypeptide with proteolytic enzyme direct hydrolysis soybean protein.But the soybean polypeptide of Production by Enzymes is often higher relatively with bitter taste and cost, and this also is the principal element of restriction enzyme process suitability for industrialized production soybean polypeptide.Wherein the inventive method utilization complex enzyme hydrolysis technology, hydrolyzed soy protein concentrate, ultra-filtration and separation make soybean polypeptide and yet there are no bibliographical information.
It is simple to the purpose of this invention is to provide a kind of technology, is suitable for the method for the method for preparing soybean polypeptide by utilizing ereyme of suitability for industrialized production.
The object of the present invention is achieved like this: a kind of preparation method of method for preparing soybean polypeptide by utilizing ereyme, and method may further comprise the steps:
1, stock liquid pre-treatment
Selecting the concentrated albumen-powder of soybeans (protein 〉=65%) for use is raw material, add 40-80 ℃ water 5-8 doubly, stir, transfer pH value 7.0-9.0,150-280 order net slurry throwing machine separates, 90-100 ℃ of 10min sterilising treatment, sterilized solution cool to 50-55 ℃ of preparation and add enzymic hydrolysis, and the oven dry of slurry throwing machine isolate is made feed and used;
2, complex enzyme hydrolysis reaction
Sterilized solution adds papoid earlier, and add-on is 0.6% of a protein powder, reacts 1-2 hour, adds compound protease reaction 10-14 hour again, and enzyme concentration is the 1-3% of protein powder, and temperature is 50-55 ℃ between the reaction period, and pH value is 9.0-6.0;
3, ultra-filtration and separation
After enzyme digestion reaction finishes, through the ultrafiltration of the following PS membrane ultrafiltration of molecular weight cut-off 6000Da device, be 40-55 ℃ in temperature, pressure is under the 0.2-0.25Mpa condition, and ultrafiltration sees through liquid and enters next procedure, and trapped fluid returns retort and continues enzymolysis.
4, sterilization, concentrated
See through liquid through 125-135 ℃ of 3-5S Ultra High Temperature Short Time, be concentrated into 40-45Bx through economic benefits and social benefits falling liquid film vacuum concentrator then.
Need to prove, in the stock liquid pre-treatment, used soybean protein concentrate, be to adopt disclosed prior art to make, can oneself produce, also can buy by market, the soybean protein concentrate of producing as Shandong Province Dongying Wan Defu vegetable-protein Science and Technology Ltd. is the used main raw material of our company.And for example, write by Liu Dachuan, Su Wangyi, the oilseed plant protein product production technology 5.5 of the 5th chapter of " edible vegetable oil and vegetable-protein " that Chemical Industry Press publishes January calendar year 2001 saves soybean protein concentrates, just describes the production principle and the production technique of soybean protein concentrate in detail.In production technique, introduced 1, washing with alcohol method reparation technology and equipment, 2, the diluted acid precipitation reparation technology.And the concentrated albumen-powder of soybeans by above any technology gained, the present invention all can use.In above complex enzyme hydrolysis reaction, can replace papoid with bromeline, also can replace papoid with the mixed enzyme of bromeline and papoid.
An optimum implementation of the present invention is: carry out spraying drying, make powdery, be about to concentrate good concentrated solution and squeeze into the press spray drying tower through high-pressure pump, at air intake 180-200 ℃, air draft 70-85 ℃, high-pressure pump pressure is to carry out drying under the 6Mpa, makes Powdered soybean polypeptide.Described feed composition contains more than the protein 65% (butt) by weight percentage, and nitrogen soluble index is more than 85%, and moisture is less than 6%, and fat is less than 0.5%.The enzyme activity of described proteolytic enzyme is: papoid 900,000 units/g, compound protease 500LAPU/g.
The soybean polypeptide that the inventive method is produced does not have obvious bitter taste, total production cost is relatively low, invest less, products made thereby not only can be directly as the high protein nutritious supplementary, also can be used as the additive of acidic vegetable protein drink, motion food, infant or baby food, military supplies food, diet food, also can be used as the main raw material of hypotensor and senior plant makeup in addition, have purposes widely.
Embodiment:
A kind of preparation method of method for preparing soybean polypeptide by utilizing ereyme comprises the steps:
1, stock liquid pre-treatment
Get the 2000g the concentrated albumen-powder of soybeans, place retort, add 8 times of clear water again, stir, transfer pH value to 8.5,200 order net filtrations see through 100 ℃ of sterilizations of liquid 10min, cool to 55 ℃, send into enzymatic vessel.Filtering filter residue oven dry is capable of using as feed.
2, enzyme digestion reaction
Claim papoid 12g (0.6%), with a small amount of protein liquid dissolving, pour into then in the whole protein liquid earlier that 55 ℃ were reacted 2 hours.This moment, enzymolysis solution PH was 6.8, took by weighing compound protease 30g (1.5%) again and directly poured in the enzymolysis solution, and 50 ℃ were reacted 10 hours, and this moment, pH value was 6.2.
3, ultra-filtration and separation
Enzymolysis solution is squeezed into the ultra-filtration and separation device (MWCO6000Da) of PS film at 50 ℃ with constant flow pump, and pressure 0.25Mpa can obtain to see through 15438 milliliters of liquid, and trapped fluid returns the continuation enzymolysis.
4, concentrate
To be concentrated to 45BX with vacuum concentrator through liquid.
5, drying
Concentrated solution through being concentrated in vacuo to 45BX is pressed into 180 ℃ of air intakes with the pressure of 6Mpa, obtains the soybean polypeptide powder of 980g in the spray-dryer that air draft is 75 ℃.
This product is measured (N * 6.25) protein 93.5% with Kjeldahl determination, and the product yield is 70.5%, and the protein following with gel chromatography analyzing molecules amount 5000Da accounts for 90% of total protein.
Described zymin is purchased in Guangzhou zymin factory and Tianjin Novo Nordisk zymin factory.
Claims (4)
1, a kind of method of method for preparing soybean polypeptide by utilizing ereyme is characterized in that described method comprises the steps:
(1) stock liquid pre-treatment
Selecting the concentrated albumen-powder of soybeans (protein 〉=65%) for use is raw material, adds 40-80 ℃ water 5-8 times, stirs, transfer pH value 7.0-9.0,150-280 order net slurry throwing machine separates, 90-100 ℃ of 10min sterilising treatment, and sterilized solution cools to 50-55 ℃ of preparation and adds enzymic hydrolysis;
(2) complex enzyme hydrolysis reaction
Sterilized solution adds papoid earlier, and add-on is 0.6% of a protein powder, reacts 1-2 hour, adds compound protease reaction 10-14 hour again, and enzyme concentration is the 1-3% of protein powder, and temperature is 50-55 ℃ between the reaction period, and pH value is 9.0-6.0;
(3) ultra-filtration and separation
After enzyme digestion reaction finishes, through the ultrafiltration of the following PS membrane ultrafiltration of molecular weight cut-off 6000Da device, be 40-55 ℃ in temperature, pressure is under the 0.2-0.25Mpa condition, and ultrafiltration sees through liquid and enters next procedure, and trapped fluid returns retort and continues enzymolysis;
(4) sterilization, concentrated
See through liquid through 125-135 ℃ of 3-5S Ultra High Temperature Short Time, be concentrated into 40-45Bx through economic benefits and social benefits falling liquid film vacuum concentrator then.
2, the method for method for preparing soybean polypeptide by utilizing ereyme according to claim 1, it is characterized in that carrying out spraying drying, promptly concentrate good concentrated solution and squeeze into the press spray drying tower through high-pressure pump, at air intake 180-200 ℃, air draft 70-85 ℃, high-pressure pump pressure is to carry out drying under the 6Mpa, makes Powdered soybean polypeptide.
3, the method for method for preparing soybean polypeptide by utilizing ereyme according to claim 1 and 2, it is characterized in that described raw material by weight percentage, comprising: soybean protein concentrate contains protein more than 65%, nitrogen soluble index (NSI) 〉=85%, moisture≤6%, fat≤0.5%.
4, the method for method for preparing soybean polypeptide by utilizing ereyme according to claim 3, the vigor that it is characterized in that papoid is 900,000 units/g, the compound protease vigor is 500LAPU/g.
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| CNB011428236A CN1135267C (en) | 2001-12-25 | 2001-12-25 | Method for preparing soybean polypeptide by utilizing ereyme method |
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| CNB011428236A CN1135267C (en) | 2001-12-25 | 2001-12-25 | Method for preparing soybean polypeptide by utilizing ereyme method |
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| CN1135267C true CN1135267C (en) | 2004-01-21 |
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| CN100463969C (en) * | 2006-03-17 | 2009-02-25 | 华南理工大学 | Process for preparing biological active peptide for improving lactic acid bacteria proliferation and fermentation to produce acid |
| CN1698879B (en) * | 2005-06-21 | 2010-08-11 | 蔺益民 | Product having sobering up and liver protecting functions and its preparation method and usage |
| CN101974591B (en) * | 2010-11-11 | 2012-07-25 | 湖北远成药业有限公司 | Enzymatic method for extracting soy peptide |
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2001
- 2001-12-25 CN CNB011428236A patent/CN1135267C/en not_active Expired - Fee Related
Cited By (3)
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|---|---|---|---|---|
| CN1698879B (en) * | 2005-06-21 | 2010-08-11 | 蔺益民 | Product having sobering up and liver protecting functions and its preparation method and usage |
| CN100463969C (en) * | 2006-03-17 | 2009-02-25 | 华南理工大学 | Process for preparing biological active peptide for improving lactic acid bacteria proliferation and fermentation to produce acid |
| CN101974591B (en) * | 2010-11-11 | 2012-07-25 | 湖北远成药业有限公司 | Enzymatic method for extracting soy peptide |
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