CN101773191B - Broad bean peptide nourishment and preparation method thereof - Google Patents
Broad bean peptide nourishment and preparation method thereof Download PDFInfo
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- CN101773191B CN101773191B CN2009103126316A CN200910312631A CN101773191B CN 101773191 B CN101773191 B CN 101773191B CN 2009103126316 A CN2009103126316 A CN 2009103126316A CN 200910312631 A CN200910312631 A CN 200910312631A CN 101773191 B CN101773191 B CN 101773191B
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Abstract
The invention relates to a broad bean peptide nourishment and a preparation method thereof, aiming to overcome the adverse factors of broad bean to human bodies, improve the conversion rate of broad bean peptide and degrade and convert high-content starch of the broad bean into oligosaccharides easily absorbed by the human bodies. The preparation method comprises the following steps of: milling the broad bean into serum after soaking and swashing, grinding and homogenizing the serum under the pressure of 600-700 kg until the fineness achieves 550 meshes - 600 meshes, stirring at 130-140 DEG Cand under the pressure of 0.6-0.7 MPa, then cooling to 40-85 DEG C, adding amylase for hydrolysis, then adding glucoamylase for hydrolysis, and finally adding neutral papain; fully stirring when the pH value is 6-8 and then standing for biochemical reaction, extracting supernatant liquid generated after the biochemical reaction, and filtering by using 11-20 micrometer vacuum micropores to obtain a basic solution of the broad bean peptide. The concentrated basic solution of the broad bean peptide can be made into nourishments of broad bean peptide powder, oral solutions, capsule or tablets and the like.
Description
Technical field
The present invention relates to utilize the beans nutriment of bioengineering processing, particularly relate to a kind of broad bean peptide nourishment and preparation method thereof.
Background technology
The Vicia legume, it contains a large amount of protein, also contain a large amount of calcium, potassium, magnesium, vitamin C etc., and the amino acid kind is comparatively complete, and particularly lysine content is abundant.Contain the important component calcium of regulating brain and nerve fiber, zinc, phosphorus etc. in the broad bean, and contain abundant choline, the brain strengthening function of enhance memory power is arranged.Rich in protein in the broad bean, and do not contain cholesterol; Vitamin C in the broad bean can delay artery sclerosis; Dietary fiber in the broad bean skin has the effect that reduces cholesterol, promotes enterocinesia.The modern thinks that also broad bean also is one of anticancer food, has prevention intestinal cancer that effect is arranged.Traditional medicine thinks that the broad bean flavor is sweet, property flat, goes into spleen, stomach warp; But tonifying middle-Jiao and Qi, invigorating spleen and reinforcing stomach, clearing heat and promoting diuresis, hemostasis step-down, relieving leukorrhea by astringents; Cure mainly the deficiency of qi in middle-jiao, burnout is eaten less, hypertension, and spitting of blood, bleeding from five sense organs or subcutaneous tissue, the women is with inferior illness.Though broad bean has so many nutritive value; But owing to contain material such as vicine in the broad bean; Cause the edible broad bean of much human irritated; Even making the people of heredity glucose-6-phosphate dehydrogenase (G6PD) (G-6-PD) defective cause side effects such as favism, problems such as the starch-containing height of broad bean, difficult processing in addition are so present people can only simply be processed into broad bean pot foods such as fried broad bean, strange taste Chinese indigo; Still belong to (slightly) process segment just of primitiveness, cause the nutritive value of broad bean to fail to be not fully exerted.
Summary of the invention
Problem to be solved by this invention is to utilize biotechnology that harmful factor in the broad bean is overcome; And the protein degradation in the broad bean generated compounds such as oligopeptide that human body is directly absorbed, small peptide, polypeptide, thereby a kind of broad bean peptide nourishment and preparation method thereof is provided.
The difficult point that addresses the above problem is: the one, how to overcome vicine of falling in the broad bean etc. has harm to human body material; The 2nd, how the high-load starch degradation of broad bean is changed into and be prone to be the glucose of absorption of human body, fructose and maltose; The 3rd, how to solve the problem that broad bean protein once-used degradeable is difficult for being converted into peptide (being that conversion ratio is lower than 8%).The applicant solves these problems through research and test preferably.
Broad bean peptide nourishment provided by the invention is that raw material is processed with the broad bean; Contain by weight: the broad bean peptide is 2～60%; The compound sugar that contains glucose, fructose and maltose is 1～30%, and all the other are lower fatty acid, vitamin, trace element, niacin, thiamine, riboflavin and water.
Above-mentioned broad bean peptide nourishment can be broad bean peptide powder, broad bean peptide oral liquid, broad bean peptide capsule or broad bean peptide tablet.
Preparation method's step of broad bean peptide nourishment provided by the invention is following:
1) will work as the broad bean of producing per year and clean the back to use weight concentration be 0.5～1.5%, temperature is 20～48 ℃ of alkali lye water loggings bubbles 15～50 hours;
2) soaked broad bean was soaked 4～10 hours with clear water, purpose is the alkali lye in the stripping broad bean, and the broad bean water after will soaking washes repeatedly, till residual alkali lye complete flushing totally;
3) cleaned broad bean is broken, then by broad bean: pure water is that 1: 8～10 weight ratio adds behind the water in slurry slag separator defibrination and extracts the broad bean protein slurry, and the filter screen fineness is 80～120 orders;
4) place the homogenizer of 600～700Kg pressure to grind homogeneous above-mentioned broad bean protein slurry, fineness reaches 550 orders～600 orders, its objective is the activity of harmful substances such as passivation vicine, promptly overcome its be prone to cause irritated be prone to cause side effects such as favism;
The broad bean protein slurry that 5) will grind after the homogenization processing is 0.6～0.7Mpa at pressure; Temperature be stir under 130～140 ℃ the condition, constant temperature handled 3～5 minutes; This process should constantly stir; In order to avoid the fully fully passivation of vicine in gelatinization and the broad bean of starch sedimentation, thereby further harmful substances such as vicine in the broad bean are carried out Passivation Treatment, thoroughly overcome it and be prone to cause irritated and the side effects such as favism that are prone to cause genetic defect to reach;
6) above-mentioned slurries are cooled to 40～85 ℃, add 0.5～3% amylase of slurry weight, stir; It in temperature hydrolysis 4～5.5 hours under 40～85 ℃ the condition; Starch hydrolysis in the broad bean is generated dextrin, carry out 3～6 hours hydrolysis after the carbohydrase that under temperature is 55～70 ℃ condition, adds slurry weight 0.5～4% then stirs, obtain containing the compound sugar (being easy to absorption of human body) of glucose, fructose and maltose; After this neutral papain that adds slurry weight 1.6～5.0 ‰ again; Under the condition of pH value 6～8, fully stir, enzyme-to-substrate is fully mixed, leave standstill again and carried out biochemical reaction in 6～14 hours;
7) extract supernatant after above-mentioned biochemical reaction is accomplished, through 11～20 microns vacuum micro porous filtrations, promptly get broad bean peptide stoste, be heated to 95～110 ℃ then, the enzyme deactivation of doing to go out is handled, and heats usually to get final product in 30～55 minutes;
The broad bean peptide liquid that 8) will go out behind the enzyme carries out concentration by the rank of demand;
9) the broad bean peptide liquid high-temperature short-time sterilization after above-mentioned the concentrating is handled;
10) the broad bean peptide liquid after will sterilizing is spray dried to broad bean peptide powder, perhaps is prepared into broad bean peptide oral liquid, broad bean peptide capsule or broad bean peptide tablet.
In step 6), add temperature that amylase is hydrolyzed and be 40～50 ℃ comparatively suitable.
In step 6), add temperature that carbohydrase is hydrolyzed and be 55～60 ℃ comparatively suitable.
The broad bean peptide stoste of the present invention's preparation is as clear as crystal; Slightly be dark brown; The conversion ratio that its broad bean protein once-used degradeable is converted into peptide can reach more than 85%; And overcome broad bean intrinsic easy initiation is irritated gets problem with side effects such as being prone to cause favism, starch is converted into more be prone to be compound sugar such as the glucose of absorption of human body, fructose and maltose, started the new way of an intensive processing for the development and use of broad bean.
The specific embodiment
Below in conjunction with specific embodiment content of the present invention is specified.
1, the broad bean that the nothing of selecting 100Kg to work as annual output is gone mouldy carries out 2 times and cleans;
2, using weight concentration is 0.5%, and temperature is that 20 ℃ of alkali lye water loggings were steeped above-mentioned broad bean 15 hours;
3, soaked broad bean, is washed 5 times with clear water after 4 hours with the clear water immersion repeatedly, alkali lye complete flushing residual on the broad bean is clean;
4, the pure water that the broad bean of rinsing well is added 800Kg after the fragmentation in disintegrating machine is through the about 900Kg of the centrifugal extraction broad bean of slurry slag separator nutrient protein slurries;
5, place the high pressure homogenizer of 600Kg pressure to grind homogeneous four times in above-mentioned slurries, make fineness reach 550 orders, carry out Passivation Treatment;
6, be 0.6Mpa with the slurries after the above-mentioned Passivation Treatment at pressure, temperature is to stir under 130 ℃ the condition, and constant temperature was handled 3 minutes, made further Passivation Treatment;
7, the slurries after the further Passivation Treatment are cooled to 40 ℃ through plate type heat exchanger;
8, will cool off the amylase that disposed slurry adds slurry weight 0.5%; Stir, make its hydrolysis 4 hours under 40 ℃ condition, carry out 3 hours hydrolysis after the carbohydrase that under temperature is 55 ℃ condition, adds slurry weight 0.5% then stirs again; Add slurry weight 1.6 ‰ neutral papains afterwards again; Be fully to stir under 6 the condition at pH value, enzyme-to-substrate is fully mixed, leave standstill then and carried out biochemical reaction in 6 hours;
9, extract the good supernatant 600Kg of biochemical reaction, promptly get the as clear as crystal dark brown broad bean peptide stoste that slightly is, be heated to 95 ℃ then and do the enzyme deactivation processing of going out of 30 minutes do through 11 microns vacuum micro porous filtrations;
About 600Kg peptide liquid that 10, will go out behind the enzyme is squeezed into double-effect falling film evaporator, by contain peptide 10% contain compound sugar such as glucose, fructose, maltose totally 5% rank concentrate and promptly get 150Kg broad bean peptide liquid;
11, above-mentioned 150Kg protein peptides liquid is handled through 1～3 second ultra high temperature short time sterilization down for 135 ℃ in temperature;
12, the broad bean peptide liquid after the sterilization treatment is squeezed into aseptic subpackaged machine and carry out packing; Promptly obtain containing broad bean peptide (oligopeptide, small peptide, polypeptide) 10%, contain compound sugar such as glucose, fructose and maltose totally 5% and abundant vitamin, trace element and multiple organic acid broad bean peptide oral liquid 150Kg (be 10ml * 15000 bottle).
Claims (4)
1. broad bean peptide nourishment; It is characterized in that with the broad bean being that raw material is processed; Contain by weight: the broad bean peptide is 2～60%; The compound sugar that contains glucose, fructose and maltose is 1～30%, and all the other are lower fatty acid, vitamin, trace element, niacin, thiamine, riboflavin and water, and described broad bean peptide nourishment is processed according to the following steps:
1) will work as the broad bean of producing per year and clean the back to use weight concentration be 0.5～1.5%, temperature is 20～48 ℃ of alkali lye water loggings bubbles 15～50 hours;
2) soaked broad bean was soaked 4～10 hours with clear water, and the broad bean water after will soaking washes repeatedly, till residual alkali lye complete flushing totally;
3) cleaned broad bean is broken, then by broad bean: pure water is that 1: 8～10 weight ratio adds behind the water in slurry slag separator defibrination and extracts the broad bean protein slurry, and the filter screen fineness is 80～120 orders;
4) place the homogenizer of 600～700Kg pressure to grind homogeneous above-mentioned broad bean protein slurry, fineness reaches 550 orders～600 orders;
The broad bean protein slurry that 5) will grind after the homogenization processing is 0.6～0.7Mpa at pressure, temperature be stir under 130～140 ℃ the condition, constant temperature handled 3～5 minutes;
6) above-mentioned slurries are cooled to 40～85 ℃, add 0.5～3% amylase of slurry weight, stir; It in temperature hydrolysis 4～5.5 hours under 40～85 ℃ the condition; Starch hydrolysis in the broad bean is generated dextrin, carry out 3～6 hours hydrolysis after the carbohydrase that under temperature is 55～70 ℃ condition, adds slurry weight 0.5～4% then stirs, obtain containing the compound sugar of glucose, fructose and maltose; After this neutral papain that adds slurry weight 1.6～5.0 ‰ again; Under the condition of pH value 6～8, fully stir, enzyme-to-substrate is fully mixed, leave standstill again and carried out biochemical reaction in 6～14 hours;
7) extract supernatant after above-mentioned biochemical reaction is accomplished, through 11～20 microns vacuum micro porous filtrations, promptly get broad bean peptide stoste, be heated to 95～110 ℃ then, the enzyme deactivation of doing to go out is handled;
The broad bean peptide liquid that 8) will go out behind the enzyme carries out concentration by the rank of demand;
9) the broad bean peptide liquid high-temperature short-time sterilization after above-mentioned the concentrating is handled;
10) the broad bean peptide liquid after will sterilizing is spray dried to broad bean peptide powder, perhaps is prepared into broad bean peptide oral liquid, broad bean peptide capsule or broad bean peptide tablet.
2. according to the preparation method of the said broad bean peptide nourishment of claim 1, it is characterized in that step is following:
1) will work as the broad bean of producing per year and clean the back to use weight concentration be 0.5～1.5%, temperature is 20～48 ℃ of alkali lye water loggings bubbles 15～50 hours;
2) soaked broad bean was soaked 4～10 hours with clear water, and the broad bean water after will soaking washes repeatedly, till residual alkali lye complete flushing totally;
3) cleaned broad bean is broken, then by broad bean: pure water is that 1: 8～10 weight ratio adds behind the water in slurry slag separator defibrination and extracts the broad bean protein slurry, and the filter screen fineness is 80～120 orders;
4) place the homogenizer of 600～700Kg pressure to grind homogeneous above-mentioned broad bean protein slurry, fineness reaches 550 orders～600 orders;
The broad bean protein slurry that 5) will grind after the homogenization processing is 0.6～0.7Mpa at pressure, temperature be stir under 130～140 ℃ the condition, constant temperature handled 3～5 minutes;
6) above-mentioned slurries are cooled to 40～85 ℃, add 0.5～3% amylase of slurry weight, stir; It in temperature hydrolysis 4～5.5 hours under 40～85 ℃ the condition; Starch hydrolysis in the broad bean is generated dextrin, carry out 3～6 hours hydrolysis after the carbohydrase that under temperature is 55～70 ℃ condition, adds slurry weight 0.5～4% then stirs, obtain containing the compound sugar of glucose, fructose and maltose; After this neutral papain that adds slurry weight 1.6～5.0 ‰ again; Under the condition of pH value 6～8, fully stir, enzyme-to-substrate is fully mixed, leave standstill again and carried out biochemical reaction in 6～14 hours;
7) extract supernatant after above-mentioned biochemical reaction is accomplished, through 11～20 microns vacuum micro porous filtrations, promptly get broad bean peptide stoste, be heated to 95～110 ℃ then, the enzyme deactivation of doing to go out is handled;
The broad bean peptide liquid that 8) will go out behind the enzyme carries out concentration by the rank of demand;
9) the broad bean peptide liquid high-temperature short-time sterilization after above-mentioned the concentrating is handled;
10) the broad bean peptide liquid after will sterilizing is spray dried to broad bean peptide powder, perhaps is prepared into broad bean peptide oral liquid, broad bean peptide capsule or broad bean peptide tablet.
3. preparation method according to claim 2 is characterized in that the temperature that adding amylase is hydrolyzed in step 6) is 40～50 ℃.
4. according to claim 2 or 3 described preparation methods, it is characterized in that the temperature that the adding carbohydrase is hydrolyzed in step 6) is 55～60 ℃.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009103126316A CN101773191B (en) | 2009-12-30 | 2009-12-30 | Broad bean peptide nourishment and preparation method thereof |
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| CN2009103126316A CN101773191B (en) | 2009-12-30 | 2009-12-30 | Broad bean peptide nourishment and preparation method thereof |
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| CN101773191B true CN101773191B (en) | 2012-05-30 |
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| CN103409403B (en) * | 2013-08-29 | 2015-06-24 | 江苏丘陵地区镇江农业科学研究所 | Method for extracting glutamic acid decarboxylase from mulberry leaves and producing soybean meal rich in polypeptides and GABA (gamma-aminobutyric acid) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1386862A (en) * | 2002-07-09 | 2002-12-25 | 邹远东 | Process for preparing cicer protein polypeptide by enzyme method and its use |
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN1932027A (en) * | 2006-09-20 | 2007-03-21 | 吉林农业大学 | Double enzyme hydrolysis process for preparing soybean peptide without bitter |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN1386862A (en) * | 2002-07-09 | 2002-12-25 | 邹远东 | Process for preparing cicer protein polypeptide by enzyme method and its use |
| CN1932027A (en) * | 2006-09-20 | 2007-03-21 | 吉林农业大学 | Double enzyme hydrolysis process for preparing soybean peptide without bitter |
Non-Patent Citations (1)
| Title |
|---|
| 邓成萍 等.双酶水解制备大豆多肽的研究.《粮油食品科技》.2006,第14卷(第1期),23-24. * |
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