CN1210409C

CN1210409C - Technological process of extracting rice protein from distiller's grains

Info

Publication number: CN1210409C
Application number: CN 02153853
Authority: CN
Inventors: 袁张度; 曹锡洪
Original assignee: Individual
Current assignee: Individual
Priority date: 2002-12-05
Filing date: 2002-12-05
Publication date: 2005-07-13
Anticipated expiration: 2022-12-05
Also published as: CN1417344A

Abstract

The present invention relates to a new technology for extracting rice proteins from monosodium glutamate leftovers. By using the technology, the rice proteins can be prepared from the monosodium glutamate leftovers by means of enzymolysis by compound enzymes (amylase, lipase, cellulase and protease). The technology uses the monosodium glutamate leftovers which are side products of monosodium glutamate plants as raw materials and has the advantages of simple step, less investment for equipment and low cost. The rice proteins can be used as food or food additives, and the absorption rate for a human body is 98%.

Description

From rice is poor, extract the novel process of rice protein

Technical field

The present invention relates to the extracting method of food, particularly relate to the novel process of from rice is poor, extracting rice protein.

Background technology

Protein is the basis of life.High-quality protein is the important substance that guarantees human health.Owing to contain each seed amino acid of needed by human body, and collocation rationally in the rice protein, and the digestibility height is so be precious complete protein.Its nutritive value is equivalent to beef, the flesh of fish, in addition because rice protein does not contain cholesterol, so its actual nutritive value and security all are better than animal proteinum.

Always rice protein all is to extract from rice bran, because operational path is long, byproduct is many, produces investment greatly, and the cost height is so be difficult to form commercialization production.

It is raw material that many Gourmet Powder Factories all use rice, produces monosodium glutamate.A large amount of rice is poor as feed applications, has caused great waste.It is about 30% to contain protein during the rice of monosodium glutamate is poor, and the rice that how to make full use of Gourmet Powder Factory's byproduct is pickled with grains or in wine and extracts rice protein, has become one of current urgent problem.

Summary of the invention

The objective of the invention is to remedy the deficiencies in the prior art part, and provide a kind of byproduct rice that utilizes poor, the enzymic hydrolysis single stage method makes the novel process of rice protein.

Goal of the invention can realize by following measure:

(1) novel process of extraction rice protein from rice is poor: the byproduct rice of Gourmet Powder Factory is pickled with grains or in wine and prozyme proportioning by weight is 1000: 1.5～4.5, and the poor enzymolysis that carries out of rice, a step promptly gets the concentrated rice protein of content 75%.

(2) prozyme is by amylase 0.05%-0.15%, lipase 0.025%-0.075%, and cellulase 0.025%-0.075% and proteinase-10 .05%-0.15% form.

(3) preferred prozyme is by amylase 0.1%, lipase 0.05%, and cellulase 0.05% and proteinase-10 .1% form.

The present invention's rice bran method compared to existing technology has following advantage:

1, raw material difference, prepared using of the present invention Gourmet Powder Factory byproduct rice is poor to be raw material, and the prior art rice bran is a raw material;

2, the present invention adopts the plurality of enzymes hydrolysis to carry out in a step, does not adopt the method for enzymolysis in the prior art;

3, two kinds of methods are different on facility investment and production cost:

By the protein of producing 1 ton of 75% content every day

The facility investment production cost

Rice bran method 5,500,000 3.8 ten thousand/ton

The rice of the present invention method 500,000 1.5 ten thousand/ton that is pickled with grains or in wine

Short because of operational path, so producing investment, the present invention is about about 10% of old technology, production cost of the present invention is about about 1/2 of old technology;

Description of drawings

Fig. 1 is a rice bran method flow process

Fig. 2 is the poor method flow process of rice.

In the rice bran method, produce the rice protein of 1 ton of content 75% with 20 tons of defatted bran slags, and produce inositol with phytic acid ca.

Embodiment

Enumerate an embodiment below, the present invention is further specified.

Embodiment 1

In the 5000L reactor, drop into the poor raw material (containing albumen about 30%) of 1 ton of rice and 5 tons of water, to transfer about pH to 3 (3.0～3.5) with 1NHcl, temperature transfers to 35～40 ℃, adds 0.5 kilogram of cellulase, reacts 30 minutes; Transfer pH to 5.0-5.5 with 30%NaOH, temperature-resistant, add 0.5 kilogram of lipase, reacted 30 minutes; Transfer pH to neutrality 7.0 with NaOH again, add 1 kilogram of amylase, 1 kilogram of proteolytic enzyme, 35～40 ℃ are reacted after 20 minutes down, are warming up to 60～70 ℃, react 30 minutes, so far finish enzyme digestion reaction.Reactant is filtered, the liquid that adds dilute alkali in the filter residue (pH 9.5) dissolving, controlled temperature obtains protein solution below 60 ℃, with acid pH is transferred to 5.5 again, makes albumen precipitation, promptly obtains the concentrated rice protein of content＞75%.Be raw material with this protein concentrate again, repeat above-mentionedly, make the step of albumen precipitation, can obtain content and be the protein isolate more than 90% with sig water dissolving with acid accent pH to 5.5.The specification 40000U/g enzyme of enzyme.

Can get the rice protein of 1.7 ton of 75% content or the protein isolate of 1 ton of 90% content with 2 tons of rice are poor.

Rice protein product of the present invention can be used as food or foodstuff additive, and the human absorptivity is 98%, its biological value＞77, (biological value of other food is: beef 75, egg 56, fish 71, shrimp 70, breast milk 81, soybean 45, wheat 41).

Claims

1, from rice is poor, extracts the novel process of rice protein, it is characterized in that the poor and prozyme proportioning by weight of the byproduct rice of Gourmet Powder Factory is 1000: 1.5～4.5; Wherein prozyme is made up of amylase 0.05%-0.15%, lipase 0.025%-0.075%, cellulase 0.025%-0.075% and proteinase-10 .05%-0.15%;

Technology is: in the poor adding of rice container, transfer PH to 3 with hydrochloric acid, temperature transfers to 35-40 ℃, adds cellulase by proportioning, reacted 30 minutes, add 30%NaOH, transfer PH5.0-5.5, add lipase, reacted 30 minutes, add NaOH again and transfer PH, add amylase, proteolytic enzyme, react and be warming up to 60-70 ℃ after 20 minutes to neutral 7.0, reacted 30 minutes, filter, add the sig water dissolving of PH9.5 in the filter residue, controlled temperature is below 60 ℃, obtain protein solution, with acid PH is transferred to 5.5 again, albumen precipitation promptly obtains the concentrated rice protein of content 75%.

2, novel process according to claim 1 is characterized in that prozyme by amylase 0.1%, lipase 0.05%, and cellulase 0.05% and proteinase-10 .1% form.