CN101215594B - Method for preparing pinctada martensii antihypertensive peptide by continuous enzyme membrane reaction - Google Patents
Method for preparing pinctada martensii antihypertensive peptide by continuous enzyme membrane reaction Download PDFInfo
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- CN101215594B CN101215594B CN2007103035125A CN200710303512A CN101215594B CN 101215594 B CN101215594 B CN 101215594B CN 2007103035125 A CN2007103035125 A CN 2007103035125A CN 200710303512 A CN200710303512 A CN 200710303512A CN 101215594 B CN101215594 B CN 101215594B
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Abstract
The invention relates to a process for preparing japanese pearl oyster antihypertensive peptides, which is characterized in that the japanese pearl oyster antihypertensive peptides are prepared through adopting alkaline protease to hydrolyze japanese pearl oyster flesh and an enzymatic membrane reactor. The process for preparation comprises following steps: disintegrating the japanese pearl oyster flesh to prepare slurry and heating until denaturation, using water to dilute and obtain reaction solution, continuingly adding the reaction solution into the enzymatic membrane reactor, reacting and separating, continuingly penetrating hydrolysis completed liquid through films and flowing out the enzymatic membrane reactor, spraying and drying the hydrolysis completed liquid, and thereby obtaining the product of japanese pearl oyster antihypertensive peptides whose protein hydrolysis yield is 40-70% and IC50 is 0.3-0.6mg/ml. The process for preparation has high product yield and low production cost and is suitable for scale production and the product can reach the requirement of general functional food.
Description
Technical field
The present invention is specifically related to a kind of preparation method of active polypeptide, particularly the method for continuous enzyme membrane prepared in reaction pinctada martensii antihypertensive peptide.
Background technology
Functional food is called as the food of 21st century, and its functions peculiar just is the adjusting to body ' s physiological rhythm of the activeconstituents that contains itself, to reach prevention or adjuvant treatment of diseases, sanatory purpose.Bioactive peptide has become functional food research that has vigor now and the frontier of processing in the fixed functional food ingredient.Blood pressure lowering peptide is a kind of important bioactive peptide, is prepared by the protease hydrolysis animal/vegetable protein.Different proteolytic enzyme, protein and preparation technology, the blood pressure lowering peptide quantity that obtains with active have bigger different." aquatic science " 2006 the 3rd phase 158-160 page or leaf has been reported and has been utilized aquatic product protein matter exploitation enzymolysis blood pressure lowering peptide, found that its antihypertensive activity of enzymolysis blood pressure lowering peptide that makes is better than other food protein from aquatic animal protein such as the flesh of fish, shrimp, crab." food and fermentation industries " 2006 the 2nd phase 113-115 page or leaf has been reported the preliminary study that pteria martensii meat ACEIP separates and extracts, the pinctada martensii antihypertensive peptide that obtains with stomach en-enzymolysis pteria martensii meat, adopt the discontinuous method enzymic hydrolysis, go out then enzyme, ultrafiltration and chromatographic separation, the bioactive peptide that obtains is 0.033mg/ml to the inhibition activity index IC50 of angiotensin-converting enzyme, but does not report the yield of blood pressure lowering peptide.
Adopting enzymatic hydrolysis reaction and membrane sepn coupled enzyme mebrane reactor is a kind of effective ways that improve the bioactive peptide yield, because the process of proteolytic enzyme protolysate matter is a non-reversible process, along with the carrying out of hydrolytic process, peptide chain constantly is cut off, and last product is an amino acid.So expect the peptide of certain seed amino acid combination, except will selecting suitable proteolytic enzyme, the more important thing is in time satisfactory peptide is separated from reaction system by film, thereby avoid being continued to decompose by enzyme, and bigger peptide and the proteolytic enzyme of molecule is stayed continuation reaction in the reactor, thereby has improved product yield and activity." chemical engineering " 2006 the 4th phase 43-46 page or leaf has been reported enzyme digestion reaction and membrane sepn coupling preparation phosphopeptide caseinate continuously, to intermittently comparing analysis with the continuous enzymolysis process, proved response and separation coupling technology can make enzymolysis efficiency and proteolytic enzyme utilization ratio significantly improve, but report does not utilize the enzyme membrane reaction to prepare the method for pinctada martensii antihypertensive peptide continuously.
Summary of the invention
The purpose of this invention is to provide the preparation method of a kind of material protein yield height, pinctada martensii antihypertensive peptide that blood pressure lowering effect is good, it is characterized in that adopting hydrolysis by novo pteria martensii meat and enzyme mebrane reactor to prepare pinctada martensii antihypertensive peptide.
Above-mentioned said Sumizyme MP is the proteolytic enzyme that Bacillus licheniformis or subtilis produced.
Above-mentioned said enzyme mebrane reactor is for to be placed on the reactor of realizing in the membrane separation unit to enzymolysis process, and the molecular weight cut-off of the membrane module of this reactor is the tubular ceramic membrane of 8-10KDa.
Performing step of the present invention and condition are as follows:
Broken slurrying of pteria martensii digested tankage and heat denatured, being diluted with water to protein content is 2-5%, and adjusting the pH value with NaOH is 9-10, obtains reaction raw materials liquid; 2, reaction raw materials liquid being added enzyme mebrane reactor continuously, is that 35-60 ℃, pressure are that 0.3-0.5MPa, basic protein enzyme concn are under the 0.2-2% in service temperature, reacts simultaneously and separates, and hydrolysis is finished liquid and seen through film continuously and flow out enzyme mebrane reactor; 3, hydrolysis is finished the liquid spraying drying, controlled spray-dired air intlet and temperature out and be respectively 170-190 ℃ and 90-110 ℃, promptly get the proteolysis yield and be 40～70%, IC50 is the pteria martensii meat decrease blood pressure peptide manufacture of 0.3～0.6mg/ml.
The present invention compares with existing technology of preparing, and its outstanding substantive distinguishing features and obvious improvement is:
(1) product yield height, production cost is low, is fit to scale production.
(2) the IC50 value of product is not passed through complicated separation, has reached lower numerical value, reaches the requirement of general utility functions food.
(3) technology is simple, and operation is continuous, less investment.
Embodiment
Embodiment one
Broken slurrying of pteria martensii digested tankage and heat denatured, being diluted with water to protein content is 3%, and adjusting the pH value with NaOH is 9, obtains reaction raw materials liquid; Reaction raw materials liquid is added the enzyme mebrane reactor that molecular weight cut-off is 8KDa continuously, in service temperature is that 45 ℃, pressure are that the concentration of 0.35MPa, proteolytic enzyme that Bacillus licheniformis produces is 0.4% time, react simultaneously and separate, hydrolysis is finished liquid and is seen through film continuously and flow out enzyme mebrane reactor; The liquid spraying drying is finished in hydrolysis, controlled spray-dired air intlet and temperature out and be respectively 180 ℃ and 100 ℃, promptly get the proteolysis yield and be 40%, IC50 is the pteria martensii meat decrease blood pressure peptide manufacture of 0.35mg/ml.
Embodiment two
Broken slurrying of pteria martensii digested tankage and heat denatured, being diluted with water to protein content is 4%, and adjusting the pH value with NaOH is 10, obtains reaction raw materials liquid; Reaction raw materials liquid is added the enzyme mebrane reactor that molecular weight cut-off is 10KDa continuously, in service temperature is that 55 ℃, pressure are that the concentration of 0.45MPa, proteolytic enzyme that subtilis produces is 1% time, react simultaneously and separate, hydrolysis is finished liquid and is seen through film continuously and flow out enzyme mebrane reactor; The liquid spraying drying is finished in hydrolysis, controlled spray-dired air intlet and temperature out and be respectively 180 ℃ and 100 ℃, promptly get the proteolysis yield and be 50%, IC50 is the pteria martensii meat decrease blood pressure peptide manufacture of 0.39mg/ml.
Embodiment three
Broken slurrying of pteria martensii digested tankage and heat denatured, being diluted with water to protein content is 4%, and adjusting the pH value with NaOH is 10, obtains reaction raw materials liquid; Reaction raw materials liquid is added the enzyme mebrane reactor that molecular weight cut-off is 10KDa continuously, in service temperature is that 50 ℃, pressure are that the concentration of 0.4MPa, proteolytic enzyme that Bacillus licheniformis produces is 0.8% time, react simultaneously and separate, hydrolysis is finished liquid and is seen through film continuously and flow out enzyme mebrane reactor; The liquid spraying drying is finished in hydrolysis, controlled spray-dired air intlet and temperature out and be respectively 180 ℃ and 100 ℃, promptly get the proteolysis yield and be 50%, IC50 is the pteria martensii meat decrease blood pressure peptide manufacture of 0.39mg/ml.
Claims (2)
1. the preparation method of a pinctada martensii antihypertensive peptide, it is characterized in that adopting hydrolysis by novo pteria martensii meat and enzyme mebrane reactor to prepare pinctada martensii antihypertensive peptide, described enzyme mebrane reactor is for to be placed on the reactor of realizing in the membrane separation unit to enzymolysis process, and the molecular weight cut-off of the membrane module of this reactor is the tubular ceramic membrane of 8-10KDa.
2. method according to claim 1, the concrete processing condition that it is characterized in that preparing pinctada martensii antihypertensive peptide are as follows:
(1) broken slurrying of pteria martensii digested tankage and heat denatured, being diluted with water to protein content is 2-5%, and adjusting the pH value with NaOH is 9-10, obtains reaction raw materials liquid;
(2) reaction raw materials liquid being added enzyme mebrane reactor continuously, is that 35-60 ℃, pressure are that 0.3-0.5MPa, basic protein enzyme concn are under the 0.2-2% in service temperature, reacts simultaneously and separates, and hydrolysis is finished liquid and seen through film continuously and flow out enzyme mebrane reactor;
(3) hydrolysis is finished the liquid spraying drying, controlled spray-dired air intlet and temperature out and be respectively 170-190 ℃ and 90-110 ℃, promptly get pteria martensii meat decrease blood pressure peptide manufacture.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007103035125A CN101215594B (en) | 2007-12-28 | 2007-12-28 | Method for preparing pinctada martensii antihypertensive peptide by continuous enzyme membrane reaction |
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| CN2007103035125A CN101215594B (en) | 2007-12-28 | 2007-12-28 | Method for preparing pinctada martensii antihypertensive peptide by continuous enzyme membrane reaction |
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| CN101215594A CN101215594A (en) | 2008-07-09 |
| CN101215594B true CN101215594B (en) | 2011-04-06 |
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Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101756237B (en) * | 2009-11-27 | 2012-03-21 | 广东海洋大学 | Method for producing Pinctada martensii meat antihypertensive functional nutrient solution |
| CN103194515B (en) * | 2013-04-02 | 2014-11-05 | 江苏科技大学 | Method for preparing silkworm pupa protein ACE (angiotensin-I converting enzyme) inhibitory peptide by continuous enzyme membrane reaction, and product and application thereof |
| CN103233054B (en) * | 2013-04-19 | 2014-07-23 | 江苏科技大学 | Method for continuously preparing silkworm chrysalis protein antioxidant peptides by using enzyme membrane reactor |
| CN109694409B (en) * | 2018-11-29 | 2021-03-19 | 中国科学院南海海洋研究所 | Angiotensin converting enzyme inhibitory activity functional peptide and application thereof |
| CN115386420A (en) * | 2022-04-20 | 2022-11-25 | 山东太爱肽生物科技股份有限公司 | Method for combined separation of peony seed oil and polypeptide |
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2007
- 2007-12-28 CN CN2007103035125A patent/CN101215594B/en not_active Expired - Fee Related
Non-Patent Citations (6)
| Title |
|---|
| 章超桦 等.马氏珠母贝肉酶解蛋白抗疲劳功能的初步研究.《中国海洋药物》.2006, |
| 章超桦等.马氏珠母贝肉酶解蛋白抗疲劳功能的初步研究.《中国海洋药物》.2006, * |
| 郝记明 等.酶解制备马氏珠母贝肉ACEIP的工艺研究.《食品科学》.2007, |
| 郝记明 等.马氏珠母贝肉ACEIP分离及提取的初步研究.《食品与发酵工业》.2006, |
| 郝记明等.酶解制备马氏珠母贝肉ACEIP的工艺研究.《食品科学》.2007, * |
| 郝记明等.马氏珠母贝肉ACEIP分离及提取的初步研究.《食品与发酵工业》.2006, * |
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Application publication date: 20080709 Assignee: Dali Hyaluronic acid Co., Ltd of Liuzhou Chemical Group Assignor: Guangxi University Contract record no.: 2011450000016 Denomination of invention: Method for preparing pinctada martensii antihypertensive peptide by continuous enzyme membrane reaction Granted publication date: 20110406 License type: Exclusive License Record date: 20110523 |
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