CN1896262A - Technology of animal high-calcium powder, chondrine and collagen by composite enzyme method - Google Patents
Technology of animal high-calcium powder, chondrine and collagen by composite enzyme method Download PDFInfo
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- CN1896262A CN1896262A CN 200610044758 CN200610044758A CN1896262A CN 1896262 A CN1896262 A CN 1896262A CN 200610044758 CN200610044758 CN 200610044758 CN 200610044758 A CN200610044758 A CN 200610044758A CN 1896262 A CN1896262 A CN 1896262A
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- chrondroitin
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- collagen protein
- calcium powder
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Abstract
The present invention relates to a biological fermentative production technology of extracting high-calcium powder, chondroitin sulfate and collagen from animal cartilage which includes the following steps: the animal cartilage is simply broken into 5-20mm fragments, mixed with 1-4 times weight of water in the reaction kettle, heated to 100DEG C and kept for 1-2 hours to denature the proteins, then cold water is passed over into the kettle jacket to cool down the kettle to 40-50DEG C, compound enzyme in mass ratio of 1:0.001 which is mainly collagenase is then added, the mixture is stirred and hydrolyzed for 8-12 hours under the condition of 40-50DEG C, pH 7-8, bone residue that contains calcium phosphate is obtained after simple filtration through 100-meshed sieves; the filtrate is clarificated after fine filtration with filter press, then ethanol is added to the final concentration of 60-70%, chondroitin will be sedimentated, while remaining liquid is the mixture of collagen and ethanol. The sedimentated calcium phosphate is washed and dried and becomes calcium phosphate powder. The crystal sediment of chondroitin is purified by ethanol-washing. Collagen is separated after the mixture of collagen and ethanol goes through the ethanol regenerating column. This technology can produce different products with high purity from animal cartilage and solves the problems of pollution and single product of traditional technology.
Description
Technical field
The present invention relates to a kind of processing technology of animal cartilage, specifically a kind of biological fermentation process extracts the production technology of high-calcium powder, chrondroitin, collagen protein from animal cartilage.
Background technology
Animal cartilage comprises the cartilage of terricole and aquatic animal now being mainly used in the extraction chrondroitin, and animal cartilage is when containing chrondroitin, and also containing has an appointment is equivalent to chrondroitin 5-10 protein doubly, wherein is collagen protein more than 90%.Extracting the existing technology of chrondroitin, all is after destroying protein with highly basic, high temperature, to isolate chrondroitin with enzymolysis process again, and ruined protein is with discharge of wastewater, and the not only unrestrained protein resource of preciousness in the animal cartilage that given up also causes serious environmental to pollute.
Summary of the invention
The purpose of this invention is to provide a kind of biological fermentation process and decompose animal cartilage, the technology of producing high-calcium powder, chrondroitin and collagen protein simultaneously changes the current domestic existing old technology that can only extract single product, reduces environmental pollution.
The objective of the invention is to realize as follows: the animal cartilage simple crushing after will cleaning becomes the 5-20mm fragment, add quality 1-4 water mixing doubly and put into reactor, being heated to 100 ℃ kept 1-2 hour, make protein denaturation, when feeding cold water is cooled to 40-50 ℃ in reacting kettle jacketing, the adding mass ratio is 1: 0.001 the prozyme based on Collagenase, keep 40-50 ℃ of temperature, pH value 7-8, stir hydrolysis 8-12 hour, obtain the bone slag of phosphoric acid calcium through 100 mesh sieve coarse filtration; The liquid that coarse filtration goes out is filtered to such an extent that clarify smart filtrate through the filter-press essence, and adding ethanol to alcohol concn is 60-70%, and chrondroitin crystalline deposit, remaining liq are the mixing solutions of collagen protein and alcohol.
Sedimentary calcium phosphate is the phosphoric acid calcium powder through cleaning, drying.
The chrondroitin crystalline deposit is with the washing with alcohol chrondroitin of purifying.
The mixing solutions of collagen protein and alcohol is isolated collagen protein behind alcohol distillation column.
From animal cartilage, separate each component with this technology, and then can produce highly purified biological high-calcium powder, chrondroitin, collagen protein, be widely used in various pharmaceutical preparations and food, in beverage and the various healthcare products, this technology has solved the seriously polluted of traditional technology existence, can only produce the drawback of single product, the theoretical yield of protein is 100%, output value rate improves more than 30%, the comprehensive pollution indexes that chrondroitin is produced descends more than 95%, acid and alkali consumption has descended more than 90%, can form great economic and social benefit, meet the industry policy guiding of " recycling economy; save society ", have very high popularizing value.
Embodiment 1:
With 1 ton of fragment that simply washes, is broken into 5-20mm of fresh ox cartilage (comprising costicartilage, trunnion), adding water mixing back adding in 1: 1 ratio wards off in the reactor, being heated to 100 ℃ kept 1-2 hour, guarantee that it is to spend that the bone piece boils, in reacting kettle jacketing, feed the cold water cooling then, when being cooled to 40-50 ℃, add Collagenase and be 1 kilogram of main prozyme, keep 40-50 ℃ of temperature, stir hydrolysis 8 hours, coarse filtration goes out the bone slag, and the bone slag gets about 200 kilograms of high-calcium powder after flushing, oven dry, pulverizing.The liquid that coarse filtration goes out essence is again filtered to such an extent that clarify smart filtrate, and this liquid adds 90% above alcohol to ethanol concn 60-70%, and the chrondroitin crystallization settles out.Liquid is the mixing solutions of collagen protein and alcohol, the chrondroitin crystallization dry after with the washing of 90% above alcohol 30 kilograms in chrondroitin powder.Be the solution of collagen protein behind the mixed solution recovery of alcohol distillation, promptly obtain Collagen Hydrolysate powder, 160 kilograms of yield through concentrating, spray to do.
Embodiment 2:
With 1 kilogram of fragment that is crushed to 5-20mm of exsiccant shark skull, ratio in 1: 4 adds entry, be heated to 100 ℃, kept 1-2 hour, be cooled to 40-50 ℃ then, adding is based on the prozyme of Collagenase, keep 40-50 ℃ temperature, stir hydrolysis 8 hours, 100 order coarse filtration go out the bone slag, the bone slag gets about 300 grams of high-calcium powder after flushing, oven dry, pulverizing; The liquid that coarse filtration goes out again essence filter the clarification smart filtrate, it is 60-70% that this liquid adds 90% above alcohol to ethanol concn, and the chrondroitin crystallization settles out, and liquid is the mixing solutions of collagen protein and alcohol, the chrondroitin crystallization gets chrondroitin powder 120 grams with 90% above alcohol washing, oven dry; Mixed solution is collagen solution behind recovery of alcohol distillation, be concentrated into 70% concentration, gets sticking cream 300 grams of honey shape.
Embodiment 3:
Step method is identical with embodiment 1,2, be raw material just with exsiccant hog snout bone, the hog snout bone contains the chrondroitin about 40%, extract with present technique, recovery rate can reach 35% (detecting finished product purity 90% through the CPC method), proof present technique coproduction chrondroitin when producing collagen protein, its yield, product purity, outward appearance all can meet or exceed the level of single production chrondroitin.
Claims (4)
1, a kind of biological fermentation process processing animals cartilage extracts high-calcium powder, chrondroitin, the production technology of collagen protein, it is characterized in that: the animal cartilage simple crushing after will cleaning becomes the 5-20mm fragment, add quality 1-4 water mixing doubly and put into reactor, being heated to 100 ℃ kept 1-2 hour, make protein denaturation, when feeding cold water is cooled to 40-50 ℃ in reacting kettle jacketing, the adding mass ratio is 1: 0.001 the prozyme based on Collagenase, keep 40-50 ℃ of temperature, pH value 7-8, stir hydrolysis 8-12 hour, obtain the bone slag of phosphoric acid calcium through 100 mesh sieve coarse filtration; The liquid that coarse filtration goes out is filtered to such an extent that clarify smart filtrate through the filter-press essence, and it is 60-70% that this liquid adds ethanol to alcohol concn, and chrondroitin crystalline deposit, remaining liq are the mixing solutions of collagen protein and alcohol.
2, biological fermentation process processing animals cartilage according to claim 1 extracts the production technology of high-calcium powder, chrondroitin, collagen protein, it is characterized in that: sedimentary calcium phosphate after filtration, clean, oven dry is the phosphoric acid calcium powder.
3, biological fermentation process processing animals cartilage according to claim 1 extracts the production technology of high-calcium powder, chrondroitin, collagen protein, it is characterized in that: the chrondroitin crystalline deposit is with the washing with alcohol chrondroitin of purifying.
4, biological fermentation process processing animals cartilage according to claim 1 extracts the production technology of high-calcium powder, chrondroitin, collagen protein, it is characterized in that: the mixing solutions of collagen protein and alcohol, and by isolating collagen protein behind the alcohol distillation column.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 200610044758 CN1896262A (en) | 2006-06-09 | 2006-06-09 | Technology of animal high-calcium powder, chondrine and collagen by composite enzyme method |
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| CN 200610044758 CN1896262A (en) | 2006-06-09 | 2006-06-09 | Technology of animal high-calcium powder, chondrine and collagen by composite enzyme method |
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| CN1896262A true CN1896262A (en) | 2007-01-17 |
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Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101913583A (en) * | 2010-08-20 | 2010-12-15 | 曹荣军 | Method for extracting chondroitin, collagen and high-calcium powder from animal cartilage |
| CN101948900A (en) * | 2010-10-09 | 2011-01-19 | 上海阿敏生物技术有限公司 | Method for extracting hydrolyzed collagen from bovine cartilage |
| CN101429531B (en) * | 2008-12-17 | 2011-08-31 | 安徽怡康生物科技有限公司 | Method for extracting biological sclerotin calcium phosphate |
| CN102382167A (en) * | 2011-11-01 | 2012-03-21 | 美泰科技(青岛)有限公司 | Method of recycling protein during production of chondroitin |
| CN102827906A (en) * | 2012-06-15 | 2012-12-19 | 四川大学 | Comprehensive extracting process for collagen type II and chondroitin sulfate |
| CN103263024A (en) * | 2013-05-10 | 2013-08-28 | 贝尔特（烟台）海洋生物科技有限公司 | Method for simultaneously producing collagens, chicken oil and high-calcium powder through using chicken bones |
| CN103397069A (en) * | 2013-08-17 | 2013-11-20 | 青岛贝尔特生物科技有限公司 | Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal |
| CN103918803A (en) * | 2013-08-07 | 2014-07-16 | 青岛博研达工业技术研究所(普通合伙) | Method for clean and high-valued extraction of chicken bones |
| CN103919149A (en) * | 2013-08-07 | 2014-07-16 | 青岛博研达工业技术研究所(普通合伙) | Method for preparation of collagen feed and combined production of chicken oil and calcium powder |
| CN104336190A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for making collagen protein film coproduction organic compounds |
| CN104336636A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method of extracting nutrients from pig bones |
| CN104336297A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for extracting organic compounds of collagen and the like from chicken bones |
| CN105420322A (en) * | 2015-12-23 | 2016-03-23 | 威海芝恩药业股份有限公司 | Method for extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen protein simultaneously |
| CN108354096A (en) * | 2018-02-06 | 2018-08-03 | 北京姿美堂生物技术有限公司 | A kind of crease-resistant moisturizing, the collagen compound beverage for improving skin elasticity |
| CN110846368A (en) * | 2019-12-10 | 2020-02-28 | 美泰科技（青岛）股份有限公司 | Clean extraction process for producing chondroitin and co-producing high-quality type II collagen from pig and ox nasal bones |
-
2006
- 2006-06-09 CN CN 200610044758 patent/CN1896262A/en active Pending
Cited By (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101429531B (en) * | 2008-12-17 | 2011-08-31 | 安徽怡康生物科技有限公司 | Method for extracting biological sclerotin calcium phosphate |
| CN101913583B (en) * | 2010-08-20 | 2012-06-27 | 曹荣军 | Method for extracting chondroitin, collagen and high-calcium powder from animal cartilage |
| CN101913583A (en) * | 2010-08-20 | 2010-12-15 | 曹荣军 | Method for extracting chondroitin, collagen and high-calcium powder from animal cartilage |
| CN101948900A (en) * | 2010-10-09 | 2011-01-19 | 上海阿敏生物技术有限公司 | Method for extracting hydrolyzed collagen from bovine cartilage |
| CN101948900B (en) * | 2010-10-09 | 2013-06-12 | 上海阿敏生物技术有限公司 | Method for extracting hydrolyzed collagen from bovine cartilage |
| CN102382167A (en) * | 2011-11-01 | 2012-03-21 | 美泰科技(青岛)有限公司 | Method of recycling protein during production of chondroitin |
| CN102827906B (en) * | 2012-06-15 | 2014-01-01 | 四川大学 | Comprehensive extracting process for collagen type II and chondroitin sulfate |
| CN102827906A (en) * | 2012-06-15 | 2012-12-19 | 四川大学 | Comprehensive extracting process for collagen type II and chondroitin sulfate |
| CN103263024B (en) * | 2013-05-10 | 2014-07-16 | 贝尔特（烟台）海洋生物科技有限公司 | Method for simultaneously producing collagens, chicken oil and high-calcium powder through using chicken bones |
| CN103263024A (en) * | 2013-05-10 | 2013-08-28 | 贝尔特（烟台）海洋生物科技有限公司 | Method for simultaneously producing collagens, chicken oil and high-calcium powder through using chicken bones |
| CN103918803B (en) * | 2013-08-07 | 2015-05-13 | 青岛博研达工业技术研究所(普通合伙) | Method for clean and high-valued extraction of chicken bones |
| CN104336297B (en) * | 2013-08-07 | 2018-05-11 | 青岛博研达工业技术研究所(普通合伙) | A kind of method for extracting the organic matter such as collagen in chicken bone |
| CN103918803A (en) * | 2013-08-07 | 2014-07-16 | 青岛博研达工业技术研究所(普通合伙) | Method for clean and high-valued extraction of chicken bones |
| CN104336190A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for making collagen protein film coproduction organic compounds |
| CN104336190B (en) * | 2013-08-07 | 2018-07-17 | 青岛博研达工业技术研究所(普通合伙) | A kind of method of glue collagen film coproduction organic matter |
| CN104336297A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method for extracting organic compounds of collagen and the like from chicken bones |
| CN103919149A (en) * | 2013-08-07 | 2014-07-16 | 青岛博研达工业技术研究所(普通合伙) | Method for preparation of collagen feed and combined production of chicken oil and calcium powder |
| CN104336636B (en) * | 2013-08-07 | 2018-07-13 | 青岛博研达工业技术研究所(普通合伙) | A method of extracting nutriment from pig bone |
| CN104336636A (en) * | 2013-08-07 | 2015-02-11 | 青岛博研达工业技术研究所(普通合伙) | Method of extracting nutrients from pig bones |
| CN103397069B (en) * | 2013-08-17 | 2015-04-22 | 青岛贝尔特生物科技有限公司 | Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal |
| CN103397069A (en) * | 2013-08-17 | 2013-11-20 | 青岛贝尔特生物科技有限公司 | Method for producing chondroitin sulfate and realizing clean co-production of protein peptides, bone oil and bone meal |
| CN105420322A (en) * | 2015-12-23 | 2016-03-23 | 威海芝恩药业股份有限公司 | Method for extracting Spanish mackerel chondroitin and Spanish mackerel bone collagen protein simultaneously |
| CN108354096A (en) * | 2018-02-06 | 2018-08-03 | 北京姿美堂生物技术有限公司 | A kind of crease-resistant moisturizing, the collagen compound beverage for improving skin elasticity |
| CN108354096B (en) * | 2018-02-06 | 2021-05-07 | 北京姿美堂生物技术有限公司 | Collagen compound beverage for resisting wrinkles, preserving moisture and improving skin elasticity |
| CN110846368A (en) * | 2019-12-10 | 2020-02-28 | 美泰科技（青岛）股份有限公司 | Clean extraction process for producing chondroitin and co-producing high-quality type II collagen from pig and ox nasal bones |
| CN110846368B (en) * | 2019-12-10 | 2021-07-02 | 美泰科技（青岛）股份有限公司 | Clean extraction process for producing chondroitin and co-producing high-quality type II collagen from pig and ox nasal bones |
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
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Open date: 20070117 |