CN1775744A - Leucine extracting method - Google Patents
Leucine extracting method Download PDFInfo
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- CN1775744A CN1775744A CNA2005101230444A CN200510123044A CN1775744A CN 1775744 A CN1775744 A CN 1775744A CN A2005101230444 A CNA2005101230444 A CN A2005101230444A CN 200510123044 A CN200510123044 A CN 200510123044A CN 1775744 A CN1775744 A CN 1775744A
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- China
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- leucine
- precipitate
- villaumite
- leucic
- extraction
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Abstract
The invention relates to a method for extracting leucine, adding chloric salt into leucine- containing fermented solution or protein hydrolysate to separate out the coarse leucine product, and then refining to obtain the leucine product. The invention adopts a compact processing course and neither uses poisonous precipitator nor uses ion exchange resin, reducing production cost, simplifying the process and shortening producing cycle on the basis of assuring the recovery and quality.
Description
Technical field
The present invention relates to a kind of leucine extracting method.
Background technology
Leucine is one of 8 kinds of indispensable amino acids of people and animal, and main the employing extracted proteolysis or fermentative Production.From protein hydrolyzate, extract leucine and mainly contain the precipitator method, ion exchange method and fermentation method, wherein the precipitator method mainly are with some organic sulfonic acids leucine precipitate and separate from hydrolyzate solution to be come out, get the leucine finished product again through making with extra care, this method is because precipitation agent costs an arm and a leg and reclaims and not exclusively causes extraction cost higher, and precipitation agent has certain toxicity, and the discharging of mother liquor has certain pollution to environment.Ion exchange method exists the shortcoming that the production cycle is long, output is little, product purity is not high, production cost is higher.Fermentation method is because bacterial classification mostly is the foreign patent technology greatly, need the expensive patent royalties of payment, the fermentation method one-time investment is big simultaneously, the production cycle long, production operation requires harshness, the fermentation back produces a large amount of by products and its separation and purification difficulty, leucine in the fermented liquid also must just can obtain qualified product through separating to purify, and causes production cost higher.
Summary of the invention
The object of the present invention is to provide a kind of production cost lower, work simplification, the leucic method of extraction that the production cycle is short.
Technical scheme of the present invention is such: the leucic method of a kind of extraction, adopt in containing leucic fermented liquid or proteolysate solution, to add villaumite, the leucine crude product is separated out, again through refining get final product the leucine product.
The present invention can realize by following process:
Stir down to reaching capacity, leave standstill adding villaumite to villaumite in leucic fermented liquid or the protein hydrolystate solution containing of pH regulator to 0～7, filter precipitate.Press precipitate: water=1: 10～100 mass ratioes are soluble in water with precipitate, add alkali and regulate pH to 4～9, press precipitate: activated carbon=10～100: 1 mass ratio adds activated carbon, 60～90 ℃ of insulated and stirred 10～50 minutes, filter destainer, the destainer condensing crystal goes out the leucine crude product, gets leucine behind the recrystallization.
After adopting such scheme, the present invention has adopted a new leucic succinct technological process of separation from fermented liquid or proteolysate solution, present method is neither used deleterious precipitation agent, do not make spent ion exchange resin yet, on the basis that guarantees the rate of recovery and quality, reduce production costs, simplify technology, shorten the production cycle.
Embodiment
Embodiment 1:
Get the protein hydrolyzate 1000L after the deacidification, regulating pH is 2, stirs to add sodium-chlor down to saturated, leaves standstill 12 hours, filter precipitate.Precipitate is dissolved in the water of 800L, adding ammoniacal liquor, to be neutralized to pH be 7, is heated to 80 ℃, adds 5Kg gac constant temperature and stir 0.5hr down, filters to get filtrate.Filtrate has been concentrated to a large amount of leucines and has separated out, and cooling is left standstill, filter the leucine crude product.The leucine crude product gets finished product behind recrystallization.
Embodiment 2:
The thalline of having got filtering and the leucine fermented liquid 1000L after decolouring, regulating pH is 2.5, stirs to add sodium-chlor down to saturated, leaves standstill 12 hours, filter precipitate.Precipitate is dissolved in the water of 1000L, adding ammoniacal liquor, to be neutralized to pH be 6, is heated to 80 ℃, adds 5Kg gac constant temperature and stir 0.5hr down, filters to get filtrate.Filtrate has been concentrated to a large amount of leucines and has separated out, and cooling is left standstill, filter the leucine crude product.Get finished product behind bright this crude product of ammonia recrystallization.
Embodiment 3:
Get the protein hydrolyzate 1000L after the deacidification, regulating pH is 2, stirs to add Repone K down to saturated, leaves standstill 12 hours, filter precipitate.Precipitate is dissolved in the water of 800L, adding ammoniacal liquor, to be neutralized to pH be 7, is heated to 80 ℃, adds 5Kg gac constant temperature and stir 0.5hr down, filters to get filtrate.Filtrate has been concentrated to a large amount of leucines and has separated out, and cooling is left standstill, filter the leucine crude product.The leucine crude product gets finished product behind recrystallization.
Embodiment 4:
Get the protein hydrolyzate 1000L after the deacidification, regulating pH is 2.5, stirs to add ammonium chloride down to saturated, leaves standstill 12 hours, filter precipitate.Precipitate is dissolved in the water of 800L, adding ammoniacal liquor, to be neutralized to pH be 7, is heated to 80 ℃, adds 5Kg gac constant temperature and stir 0.5hr down, filters to get filtrate.Filtrate decompression has been concentrated to a large amount of leucines and has separated out, and cooling is left standstill, filter the leucine crude product.The leucine crude product gets finished product behind recrystallization.
Claims (3)
1, the leucic method of a kind of extraction, it is characterized in that: realize: stir down to pH regulator is added villaumite to villaumite containing between 0～7 in leucic fermented liquid or the protein hydrolystate solution and reach capacity by following process, leave standstill, filter precipitate; Press precipitate: the mass ratio of water=1: 10～100 is soluble in water with precipitate, add alkali and regulate pH to 4～9, press precipitate: activated carbon=10～100: 1 mass ratio adds activated carbon, 60～90 ℃ of insulated and stirred 10～50 minutes, filter destainer, the destainer condensing crystal goes out the leucine crude product, gets leucine behind the recrystallization.
2, the leucic method of a kind of extraction as claimed in claim 1, it is characterized in that: above-mentioned villaumite is a sodium-chlor.
3, the leucic method of a kind of extraction as claimed in claim 1, it is characterized in that: above-mentioned villaumite is a Repone K.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005101230444A CN1775744A (en) | 2005-12-08 | 2005-12-08 | Leucine extracting method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2005101230444A CN1775744A (en) | 2005-12-08 | 2005-12-08 | Leucine extracting method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1775744A true CN1775744A (en) | 2006-05-24 |
Family
ID=36765484
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2005101230444A Pending CN1775744A (en) | 2005-12-08 | 2005-12-08 | Leucine extracting method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1775744A (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922951A (en) * | 2014-05-06 | 2014-07-16 | 鲁成宪 | Method for extracting leucine by utilizing corn protein powder |
| CN104926670A (en) * | 2015-06-02 | 2015-09-23 | 江苏三宝药业有限公司 | Method for extracting L-leucine from vegetable protein |
| CN110330441A (en) * | 2019-08-19 | 2019-10-15 | 精晶药业股份有限公司 | A kind of method of purification of D-Leu |
| CN114560782A (en) * | 2022-02-10 | 2022-05-31 | 无锡晶海氨基酸股份有限公司 | Preparation method of high-purity leucine |
-
2005
- 2005-12-08 CN CNA2005101230444A patent/CN1775744A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103922951A (en) * | 2014-05-06 | 2014-07-16 | 鲁成宪 | Method for extracting leucine by utilizing corn protein powder |
| CN103922951B (en) * | 2014-05-06 | 2015-06-17 | 鲁成宪 | Method for extracting leucine by utilizing corn protein powder |
| CN104926670A (en) * | 2015-06-02 | 2015-09-23 | 江苏三宝药业有限公司 | Method for extracting L-leucine from vegetable protein |
| CN104926670B (en) * | 2015-06-02 | 2017-03-29 | 江苏三宝药业有限公司 | It is a kind of to extract the leucic methods of L from vegetable protein |
| CN110330441A (en) * | 2019-08-19 | 2019-10-15 | 精晶药业股份有限公司 | A kind of method of purification of D-Leu |
| CN110330441B (en) * | 2019-08-19 | 2022-03-11 | 精晶药业股份有限公司 | Purification method of D-leucine |
| CN114560782A (en) * | 2022-02-10 | 2022-05-31 | 无锡晶海氨基酸股份有限公司 | Preparation method of high-purity leucine |
| CN114560782B (en) * | 2022-02-10 | 2024-03-01 | 无锡晶海氨基酸股份有限公司 | Preparation method of high-purity leucine |
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| C06 | Publication | ||
| PB01 | Publication | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20060524 |