CN102134260A - Method for preparing phytic acid - Google Patents

Abstract

The invention discloses a method for preparing phytic acid, which comprises: adding dry defatted rice bran into 2-percent HCl solution, soaking with constant stirring, filtering to obtain primary filtrate, adding diluted HCl into filter residue, constantly stirring and impregnating, filtering secondary extract, washing the filter residue with a small amount of water, and obtaining mixed extract and washing liquor; passing the mixed extract through an anion exchange resin to absorb phytin, washing the resin with countercurrent of warm water to remove proteins and sugars, washing off inorganic phosphorus and other impurities with NaCl solution to obtain sodium phytate eluant; adding strongly acidic cation exchange resin into the sodium phytate eluant, stirring, regulating, filtering, washing the resin with deionized water, mixing the washing liquor with filtrate, and obtaining effluent, namely diluted phytic acid, after the phytate flows through the exchange column; and concentrating the diluted phytic acid by half volume, decolorizing with active carbon and continuously concentrating under reduced pressure to obtain phytic acid solution at a concentration of 71 percent, wherein the extraction rate is 84.6 percent. The method has the advantages of simple process, low cost and high product yield and quality.

Description

A kind of preparation method of phytic acid

Technical field:

The present invention relates to a kind of preparation method of phytic acid.Be applied to fields such as medicine, food, chemical industry, metallurgy, light industry.

Technical background:

Phytic acid [C ₆H ₁₈O ₂₄P ₆] have special physiological properties and chemical functional, be subject to people's attention more and more.From rice bran or maize germ, extract phytic acid and have important economic value, in 20th century the eighties list in the national spark program.

Many researchs of having produced of relevant phytic acid are reported, the tradition method for making is all passed through the neutralization precipitation step, promptly extract with diluted acid earlier, use liming caustic soda again, ammoniacal liquor etc. are settled out phytin, this moment phytin in the pasty state thing separate out protein, impurity such as carbohydrate precipitate together in a large number, contain a certain amount of acid or inorganic phosphate in the diluted acid extraction liquid simultaneously, when making precipitation agent with lime, the insoluble calcium phosphate magnesium salts that generates is in company with the phytin coprecipitation, so that want to remove effectively relatively difficulty of these impurity, not only increased the subsequent disposal burden but also influenced the output and the quality of phytic acid product.

Summary of the invention;

It is simple to the purpose of this invention is to provide a kind of technology, cost reduction, the yield of product and the preparation method of a kind of phytic acid that quality all can improve.

In order to solve the problems of the technologies described above, the invention provides a kind of preparation method of phytic acid, the steps include:

Step 1, the exsiccant defatted rice bran is added 2%HCl solution 6L regulate pH value to 3-4, under constantly stirring, soak 3-4h, the filtration time filtrate won of slagging tap, add 1% rare HCl solution 3L in the filter residue and regulate pH value to 4-5, the continuous stirring washed filter residue 1-2 time with less water after dipping 3-4h filters the vat liquor second time, makes to merge vat liquor and washing lotion;

The exchange of step 2, resin anion(R.A): with above-mentioned amalgamation liquid with 3-4L speed per hour by 717 type anionite-exchange resin with the absorption phytin, with 40-50 ℃ of this resin of warm water countercurrent washing to remove deproteinize and carbohydrate, use the foreign material such as speed flush away inorganic phosphorus of 0.15mol/L NaCl again, obtain 1.5L sodium phytate eluant with 0.7mol/L NaCl with the speed wash-out of 2L/h then with 2L/h;

Step 3, resin cation exchange: in above-mentioned sodium phytate eluant, add the 0.1L storng-acid cation exchange resin, the stirring and adjusting pH value filters to 5-6 and uses the deionized water wash resin, washing lotion is incorporated filtrate into, filtrate is broken through 0.4L 732 type Zeo-karbs with 1-2L/h speed, when phytate is flowed through this exchange column, the Na in the solution ⁺With H ^-Exchange, effluent liquid is thin planting acid;

Step 4, decolouring concentrate: above-mentioned dilute phytic acid solution concentrated half that reduces to volume, use activated carbon decolorizing, and the continuation concentrating under reduced pressure, temperature is controlled at≤70 ℃, obtains content and be 71% plant acid solution, and extraction yield is 84.6%.

Described resin anion(R.A) is Cl ^-Type.

The present invention has following remarkable advantages and beneficial effect:

1) technology is simple, and cost reduces;

2) yield of product and quality height.

Embodiment:

Preparation method to a kind of phytic acid further is illustrated by the following examples:

Step 1, take by weighing exsiccant defatted rice bran 1000g and add 2%HCl solution 6L and regulate pH value to 3-4, under constantly stirring, soak 3-4h, the filtration time filtrate won of slagging tap, add 1% rare HCl solution 3L in the filter residue and regulate pH value to 4-5, constantly stir dipping 3-4h and filter for the second time after the vat liquor with less water washing filter residue 1-2 time, merge vat liquor and washing lotion be total to 10L;

The exchange of step 2, resin anion(R.A): (this resin is anticipated and is Cl by 0.7L717 type anionite-exchange resin with 3-4L speed per hour with above-mentioned amalgamation liquid ^-Type) with the absorption phytin, with 40-50 ℃ of this resin of warm water countercurrent washing to remove deproteinize and carbohydrate, use the foreign material such as speed flush away inorganic phosphorus of 0.15mol/L NaCl again, obtain 1.5L sodium phytate eluant with 0.7mol/L NaCl with the speed wash-out of 2L/h then with 2L/h;

Step 3, resin cation exchange: in above-mentioned phytic acid eluant, add the 0.1L storng-acid cation exchange resin, the stirring and adjusting pH value filters to 5-6 and uses the deionized water wash resin, washing lotion is incorporated filtrate into, filtrate is broken through 0.4L 732 type Zeo-karbs with 1-2L/h speed, when phytate is flowed through this exchange column, the Na in the solution ⁺With H ^-Exchange, effluent liquid is thin planting acid;

Step 4, decolouring concentrate: above-mentioned dilute phytic acid solution concentrated half that reduces to volume, use activated carbon decolorizing, and the continuation concentrating under reduced pressure, temperature is controlled at≤and 70 ℃, obtaining content is 71%, plant acid solution 85.8g, extraction yield are 84.6%.

It should be noted that at last: above embodiment only in order to the explanation the present invention and and unrestricted technical scheme described in the invention; Therefore, although this specification sheets has been described in detail the present invention with reference to each above-mentioned embodiment,, those of ordinary skill in the art should be appreciated that still and can make amendment or be equal to replacement the present invention; And all do not break away from the technical scheme and the improvement thereof of the spirit and scope of invention, and it all should be encompassed in the middle of the claim scope of the present invention.

Claims (2)

1. the preparation method of a phytic acid, it is characterized in that: its preparation process is:

Step 1, the exsiccant defatted rice bran is added 2%HCl solution 6L regulate pH value to 3-4, under constantly stirring, soak 3-4h, the filtration time filtrate won of slagging tap, add 1% rare HCl solution 3L in the filter residue and regulate pH value to 4-5, the continuous stirring washed filter residue 1-2 time with less water after dipping 3-4h filters the vat liquor second time, makes to merge vat liquor and washing lotion;

The exchange of step 2, resin anion(R.A): with above-mentioned amalgamation liquid with 3-4L speed per hour by 717 type anionite-exchange resin with the absorption phytin, with 40-50 ℃ of this resin of warm water countercurrent washing to remove deproteinize and carbohydrate, use the foreign material such as speed flush away inorganic phosphorus of 0.15mol/L NaCl again, obtain 1.5L sodium phytate eluant with 0.7mol/L NaCl with the speed wash-out of 2L/h then with 2L/h;

Step 3, resin cation exchange: in above-mentioned sodium phytate eluant, add the 0.1L storng-acid cation exchange resin, the stirring and adjusting pH value filters to 5-6 and uses the deionized water wash resin, washing lotion is incorporated filtrate into, filtrate is broken through 0.4L 732 type Zeo-karbs with 1-2L/h speed, when phytate is flowed through this exchange column, the Na in the solution ⁺With H ^-Exchange, effluent liquid is thin planting acid;

Step 4, decolouring concentrate: above-mentioned dilute phytic acid solution concentrated half that reduces to volume, use activated carbon decolorizing, and the continuation concentrating under reduced pressure, temperature is controlled at≤70 ℃, obtains content and be 71% plant acid solution, and extraction yield is 84.6%.

2. the preparation method of a kind of phytic acid as claimed in claim 1, it is characterized in that: described resin anion(R.A) is Cl ^-Type.