CN110592168A - Method for preparing small molecule peptide - Google Patents
Method for preparing small molecule peptide Download PDFInfo
- Publication number
- CN110592168A CN110592168A CN201910930999.2A CN201910930999A CN110592168A CN 110592168 A CN110592168 A CN 110592168A CN 201910930999 A CN201910930999 A CN 201910930999A CN 110592168 A CN110592168 A CN 110592168A
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- China
- Prior art keywords
- small molecule
- heating
- protease
- molecule peptide
- producing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for preparing small molecular peptide, which comprises the steps of putting protein raw materials, water and protease into an enzymolysis tank for emulsification for 1 hour, heating to 58 ℃ for enzymolysis for 10 hours, heating to 90 ℃ for stirring for 1 hour, sieving with a 300-mesh sieve at 75 ℃ to 90 ℃, decoloring, deodorizing by a centrifugal machine, concentrating, and spray-drying to obtain the small molecular peptide. The invention has low production cost, zero pollution and no environmental protection pressure; the production period is short, the production process flow is simple and easy to operate, and the efficiency is high.
Description
Technical Field
The invention belongs to the technical field of food health care, and particularly relates to a preparation method of a small molecular peptide.
Background
The protein peptide not only has various human metabolism and physiological regulation functions, but also can provide nutrition for organisms, has the physiological functions of resisting bacteria and viruses, reducing blood pressure, reducing blood fat, enhancing immunity and the like, has extremely high edible safety, and is the most popular research topic of the current food science and technology and a functional factor with great development prospect. At present, small molecular protein peptide is mainly extracted from plants or animals, the extraction process is complex, the extraction efficiency is low, and the production period is long.
Disclosure of Invention
The invention aims to provide a method for preparing small molecular peptide, which has low production cost, zero pollution and no environmental protection pressure; the production period is short, the production process flow is simple and easy to operate, and the efficiency is high.
In order to achieve the purpose, the invention is realized by the following technical scheme: a process for preparing small-molecular peptide includes such steps as adding protein, water and proteinase to enzymolysis tank, emulsifying for 1 hr, heating to 58 deg.C for 10 hr, heating to 90 deg.C, stirring for 1 hr, sieving by 300 meshes at 75-90 deg.C, centrifugal separation, removing odour, concentrating and spray drying.
In a specific embodiment of the invention, the weight parts of the protein raw material, the water and the protease are respectively 1000 parts, 5000 parts and 10 parts.
In a specific embodiment of the invention, the protein raw materials comprise moringa seeds, bean flour, walnuts, corns and wheat.
In a specific embodiment of the present invention, the protease includes bromelain, papain, flavourzyme, and neutral protease.
In one embodiment of the present invention, the centrifuge is a disk centrifuge.
Compared with the prior art, the invention has the following beneficial effects: the invention has low production cost, zero pollution and no environmental protection pressure; the production period is short, the production process flow is simple and easy to operate, and the efficiency is high.
Detailed Description
Other aspects, features and advantages of the present invention will become apparent from the following detailed description, which, when taken in conjunction with the drawings, illustrate by way of example the principles of the invention.
A process for preparing small molecular peptide includes such steps as loading 1000kg of protein material, 5000kg of water and 10kg of protease into enzymolysis tank, emulsifying for 1 hr, heating to 58 deg.C for enzymolysis for 10 hr, heating to 90 deg.C, stirring for 1 hr, sieving by 300 meshes at 75-90 deg.C, decoloring and deodoring by disk-type centrifugal machine, vacuum concentrating, and spray drying. Wherein the protein raw materials comprise moringa seed, bean flour, walnut, corn and wheat, and the protease comprises bromelain, papain, flavourzyme and neutral protease.
Specifically, the disk centrifuge used in the invention is an automatic slag discharge separation of three-phase separation (water, oil and slag), and plays a role in decoloring and deodorizing. The enzymolysis tank used in the invention has the functions of temperature, pressure and stirring.
While the foregoing is directed to the preferred embodiment of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow.
Claims (5)
1. A process for preparing small-molecular peptide includes such steps as adding protein, water and proteinase to an enzymolysis tank, emulsifying for 1 hr, heating to 58 deg.C for 10 hr, heating to 90 deg.C, stirring for 1 hr, sieving at 75-90 deg.C with 300-mesh sieve, centrifugal decoloring, deodouring, concentrating and spray drying.
2. The method for producing a small molecule peptide according to claim 1, wherein the weight parts of the protein material, water and the protease are 1000 parts, 5000 parts and 10 parts, respectively.
3. The method for producing a small molecule peptide according to claim 2, wherein the protein material comprises moringa seeds, bean flour, walnuts, corn, wheat.
4. The method for producing a small molecule peptide according to claim 2, wherein the protease includes bromelain, papain, flavourzyme, and neutral protease.
5. The method for producing a small molecule peptide according to claim 1, wherein the centrifuge is a disk centrifuge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910930999.2A CN110592168A (en) | 2019-09-29 | 2019-09-29 | Method for preparing small molecule peptide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910930999.2A CN110592168A (en) | 2019-09-29 | 2019-09-29 | Method for preparing small molecule peptide |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110592168A true CN110592168A (en) | 2019-12-20 |
Family
ID=68864371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910930999.2A Pending CN110592168A (en) | 2019-09-29 | 2019-09-29 | Method for preparing small molecule peptide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110592168A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN101824455A (en) * | 2009-03-06 | 2010-09-08 | 香港百特有限公司 | Soy protein oligopeptide, and preparation method and use thereof |
| CN103103241A (en) * | 2011-11-14 | 2013-05-15 | 广东好普多肽生物科技有限公司 | Method for preparing corn oligopeptide by using zein as raw material |
-
2019
- 2019-09-29 CN CN201910930999.2A patent/CN110592168A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN101824455A (en) * | 2009-03-06 | 2010-09-08 | 香港百特有限公司 | Soy protein oligopeptide, and preparation method and use thereof |
| CN103103241A (en) * | 2011-11-14 | 2013-05-15 | 广东好普多肽生物科技有限公司 | Method for preparing corn oligopeptide by using zein as raw material |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191220 |