CN101988080A - Method for producing soybean small peptide - Google Patents
Method for producing soybean small peptide Download PDFInfo
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- CN101988080A CN101988080A CN2009100173781A CN200910017378A CN101988080A CN 101988080 A CN101988080 A CN 101988080A CN 2009100173781 A CN2009100173781 A CN 2009100173781A CN 200910017378 A CN200910017378 A CN 200910017378A CN 101988080 A CN101988080 A CN 101988080A
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- liquid
- small peptide
- soybean protein
- protein isolate
- semen sojae
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Abstract
The invention provides a method for producing a soybean small peptide, comprising the following steps of: 1, washing; 2, protein liquid thermal treatment; 3, enzymolysis, wherein the soybean separated protein liquid after the thermal treatment is hydrolyzed by a compound protease; 4, centrifugal precipitation; 5, macromolecular proteins removal, wherein the soybean small peptide liquid is ultra-filtered through a polyvinyl chloride hollow fiber membrane with the interception molecular weight of 10,000 daltons so as to remove macromolecular proteins; 6, concentration; and 7, drying. In the method for producing the soybean small peptide, the enzymolysis time can be shortened to be about 4 hours due to the usage of the compound protease; in addition, the compound protease can be synchronously acted in different sites connected by amino acids in the proteins during the enzymolysis, thus shortening the enzymolysis time, improving the enzymolysis efficiency, greatly reducing the cost, reducing, even preventing the generation of bitterness. Furthermore, an ultra-filter technique is utilized, so the solution of the soybean small peptide is clear and transparent. The protein can be directly absorbed by human bodies without passing through the digestive system due to small molecular weight of the protein; and therefore, the adsorption is fast, and the nutrition is abundant.
Description
Technical field
The invention provides a kind of soybean deep processing method, especially a kind of production method of Semen sojae atricolor small peptide.
Background technology
Semen sojae atricolor small peptide is meant soybean protein isolate behind protease hydrolysis, again the protein hydrolyzate that obtains through process such as separating, refining.Its multiple peptide mixt after by soybean protein hydrolysis is formed jointly, based on small-molecular peptides, also contains compositions such as a small amount of macromole peptide, total free aminoacids, carbohydrate and inorganic salt.The protein content of Semen sojae atricolor small peptide is more than 90%, and its amino acid is formed identical with soy-protein, and necessary amino acid whose balance is good, and content is abundant.Semen sojae atricolor small peptide water-soluble very high is even still have flowability under 50% high density; Have stronger water absorbability and moisture retention, arrestin matter form gel, adjust protein food product hardness, improve mouthfeel and characteristic such as easily digest and assimilate.Little peptide in the diet (2-3 amino acid) and big peptide (10-51 amino acid) can intactly pass through enteron aisle, are directly absorbed by enteron aisle, and little peptide absorption rate is faster than the total free aminoacids of same composition, and the protein utilization height.Semen sojae atricolor small peptide also has vegetative nervous system, the active cells immunologic function of adjusting, improves cardiovascular function and the anti-ageing physiologically active of waiting for a long time.And the Semen sojae atricolor small peptide antigenicity is low, can not produce anaphylaxis.It has been found that in recent years Semen sojae atricolor small peptide also has many medical science functions, as reducing cholesterol, inhibition elevation of blood pressure, raising nonallergic, raise immunity and Green Tea Extract damage (delaying senility) etc.
Soy proteinaceous hydrolysis reaction can be undertaken by acid, alkali or enzyme catalysis, and acid, base catalysis technology have bigger destruction because its reaction is violent to nutritive ingredient.Adopting enzyme process catalysis is the main method that present biologically active peptides is produced.But the single enzyme of this employing is hydrolyzed, and can make reasons such as the intramolecular hydrophobic amino acid residue exposure of soybean protein, makes gained soybean peptides product that comparatively serious bitter taste be arranged, and has limited the application of soybean peptides product.In order to remove the bitter taste of soybean peptides product, people have developed multiple debitterized technique and have comprised absorption, shelter etc., and independent debitterized technique unavoidably makes complex process, and cost increases, and productive rate is descended.
Summary of the invention
Technical problem to be solved by this invention is, a kind of as clear as crystal Semen sojae atricolor small peptide production method of production method of Semen sojae atricolor small peptide is provided, and to improve the quality of Semen sojae atricolor small peptide, reduces or eliminates its bitter taste.
The present invention is achieved in that a kind of production method of Semen sojae atricolor small peptide, and the step of described method is:
(1) washing
With the lyolysis of soybean protein isolate curdled milk broken after, add 6～10 times to the deionized water of soybean protein isolate curdled milk liquid weight, fully stir the back centrifugation, the deionized water that adds 4～6 times of soybean protein isolate weight that obtain to centrifugal back again, adjusting pH value with sodium hydroxide solution is 8～9, under 45～50 ℃ of temperature, stirred 13～18 minutes, get soybean protein isolate liquid;
(2) protein liquid thermal treatment
It is 85～90 ℃ that above-mentioned soybean protein isolate liquid is heated to temperature, at the uniform velocity stirs 15-20 minute with 60～80 rev/mins speed;
(3) enzymolysis
To add hydrolysis reactor through the soybean protein isolate liquid after the above-mentioned thermal treatment, in 5～10 minutes, soybean protein isolate liquid is cooled to 45～50 ℃, being adjusted to pH value with sodium hydroxide solution is 8～9, the compound protease of soybean protein solution volume thousandth to three is added in the hydrolysis reactor, temperature remains 45～50 ℃, enzymolysis 3.5～5 hours gets mixed peptide liquid; After reacting time of arrival, mixed peptide liquid was warming up to 85～95 ℃ in 5～10 minutes, kept 5～10 minutes, compound protease is lost activity;
(4) centrifugation
To collect supernatant liquor and get Semen sojae atricolor small peptide liquid through the mixed peptide liquid of above-mentioned processing under 4000 rev/mins rotating speed centrifugal 8～12 minutes;
(5) remove high molecular weight protein
Is 10000 daltonian PVC hollow fiber membrane ultrafiltration with Semen sojae atricolor small peptide liquid through the molecular weight that dams, and removes macromolecular albumen;
(6) concentrate
Carry out two and imitate or triple effect concentrates removing Semen sojae atricolor small peptide behind the high molecular weight protein, its volume is concentrated into original 1/2nd to 1/4th;
(7) drying
Spraying drying promptly gets the Semen sojae atricolor small peptide product.
The production method of this Semen sojae atricolor small peptide adopts compound protease, and enzymolysis time can shorten to about 4 hours, and compound protease acts on the different loci that amino acid connects in the protein simultaneously during enzymolysis, shorten enzymolysis time, improve enzymolysis efficiency, reduced cost significantly.Owing to adopted compound protease to be hydrolyzed, avoided adopting single enzyme to be hydrolyzed, make problems such as the intramolecular hydrophobic amino acid residue exposure of soybean protein, alleviate even avoided the generation of bitter taste.Adopt ultra-filtration technique simultaneously, Semen sojae atricolor small peptide solution clear.According to the Semen sojae atricolor small peptide that the present invention's method is produced, protein molecular weight is little, can directly not be absorption of human body by Digestive tract, absorbs fast, and is nutritious.
Embodiment
Further specify the present invention below.
The Semen sojae atricolor small peptide product that the present invention's method makes is shallow white and slightly yellowy powder, and bitter taste is arranged slightly.Its amino acid whose composition is almost completely the same with soy-protein, also has amino acid ratio balance, the characteristics that content is abundant.Its quality index is as follows:
(1) Oranoleptic indicator
Color and luster: white is yellow powder slightly.
Flavour and smell: have distinctive flavour of this product and smell, do not have other peculiar smell.
(2) physical and chemical index
Crude protein (in butt, reduction factor is 6.25): 92%
Relative molecular mass distribution/u＜1000 of 〉=80% peptide section
Peptide content (in butt): 82.5%
Moisture content: 6.5%
Ash oontent: 6.4%
Trichoroacetic acid(TCA) soluble proteins 99.9%
It is 30% sodium hydroxide solution that described sodium hydroxide solution can adopt mass percent concentration.
Described soybean protein curdled milk liquid is the intermediates in the soybean protein isolate production process.
Described " lyolysis of soybean protein isolate curdled milk is broken " finished with machine for decomposing and smashing.
Embodiment 1
A kind of production method of Semen sojae atricolor small peptide, the step of described method is:
(1) washing
With the lyolysis of soybean protein isolate curdled milk broken after, add 6 times to the deionized water of soybean protein isolate curdled milk liquid weight, fully stir the back centrifugation, the deionized water that adds 6 times of soybean protein isolate weight that obtain to centrifugal back again, adjusting the pH value with sodium hydroxide solution is 8, under 50 ℃ of temperature, stirred 13 minutes, get soybean protein isolate liquid;
(2) protein liquid thermal treatment
It is 90 ℃ that above-mentioned soybean protein isolate liquid is heated to temperature, at the uniform velocity stirs 20 minutes with 60 rev/mins speed;
(3) enzymolysis
To add hydrolysis reactor through the soybean protein isolate liquid after the above-mentioned thermal treatment, in 5 minutes, soybean protein isolate liquid is cooled to 50 ℃, being adjusted to the pH value with sodium hydroxide solution is 8, the compound protease of soybean protein solution volume 3/1000ths is added in the hydrolysis reactor, temperature remains 45 ℃, enzymolysis 5 hours gets mixed peptide liquid; After reacting time of arrival, mixed peptide liquid was warming up to 95 ℃ in 5 minutes, kept 5 minutes, compound protease is lost activity;
(4) centrifugation
To collect supernatant liquor and get Semen sojae atricolor small peptide liquid through the mixed peptide liquid of above-mentioned processing under 4000 rev/mins rotating speed centrifugal 12 minutes;
(5) remove high molecular weight protein
Is 10000 daltonian PVC hollow fiber membrane ultrafiltration with Semen sojae atricolor small peptide liquid through the molecular weight that dams, and removes macromolecular albumen;
(6) concentrate
The Semen sojae atricolor small peptide of removing behind the high molecular weight protein is carried out two effects, its volume is concentrated into original 1/4th;
(7) drying
Spraying drying promptly gets the Semen sojae atricolor small peptide product.
Embodiment 2
A kind of production method of Semen sojae atricolor small peptide, the step of described method is:
(1) washing
With the lyolysis of soybean protein isolate curdled milk broken after, add 10 times to the deionized water of soybean protein isolate curdled milk liquid weight, fully stir the back centrifugation, the deionized water that adds 4 times of soybean protein isolate weight that obtain to centrifugal back again, adjusting the pH value with sodium hydroxide solution is 9, under 45 ℃ of temperature, stirred 18 minutes, get soybean protein isolate liquid;
(2) protein liquid thermal treatment
It is 85 ℃ that above-mentioned soybean protein isolate liquid is heated to temperature, at the uniform velocity stirs 15 minutes with 80 rev/mins speed;
(3) enzymolysis
To add hydrolysis reactor through the soybean protein isolate liquid after the above-mentioned thermal treatment, in 10 minutes, soybean protein isolate liquid is cooled to 45 ℃, being adjusted to the pH value with sodium hydroxide solution is 9, the millesimal compound protease of soybean protein solution volume is added in the hydrolysis reactor, temperature remains 50 ℃, enzymolysis 3.5 hours gets mixed peptide liquid; After reacting time of arrival, mixed peptide liquid was warming up to 85 ℃ in 10 minutes, kept 10 minutes, compound protease is lost activity;
(4) centrifugation
To collect supernatant liquor and get Semen sojae atricolor small peptide liquid through the mixed peptide liquid of above-mentioned processing under 4000 rev/mins rotating speed centrifugal 8 minutes;
(5) remove high molecular weight protein
Is 10000 daltonian PVC hollow fiber membrane ultrafiltration with Semen sojae atricolor small peptide liquid through the molecular weight that dams, and removes macromolecular albumen;
(6) concentrate
Carry out triple effect and concentrate removing Semen sojae atricolor small peptide behind the high molecular weight protein, its volume is concentrated into original 1/2nd;
(7) drying
Spraying drying promptly gets the Semen sojae atricolor small peptide product.
Claims (1)
1. the production method of a Semen sojae atricolor small peptide is characterized in that, the step of described method is:
(1) washing
With the lyolysis of soybean protein isolate curdled milk broken after, add 6～10 times to the deionized water of soybean protein isolate curdled milk liquid weight, fully stir the back centrifugation, the deionized water that adds 4～6 times of soybean protein isolate weight that obtain to centrifugal back again, adjusting the pH value with sodium hydroxide solution is 8～9, under 45～50 ℃ of temperature, stirred 13～18 minutes, get soybean protein isolate liquid;
(2) protein liquid thermal treatment
It is 85～90 ℃ that above-mentioned soybean protein isolate liquid is heated to temperature, at the uniform velocity stirs 15-20 minute with 60～80 rev/mins speed;
(3) enzymolysis
To add hydrolysis reactor through the soybean protein isolate liquid after the above-mentioned thermal treatment, in 5～10 minutes, soybean protein isolate liquid is cooled to 45～50 ℃, being adjusted to the pH value with sodium hydroxide solution is 8～9, the compound protease of soybean protein solution volume thousandth to three is added in the hydrolysis reactor, temperature remains 45～50 ℃, enzymolysis 3.5～5 hours gets mixed peptide liquid; After reacting time of arrival, mixed peptide liquid was warming up to 85～95 ℃ in 5～10 minutes, kept 5～10 minutes, compound protease is lost activity;
(4) centrifugation
To collect supernatant liquor and get Semen sojae atricolor small peptide liquid through the mixed peptide liquid of above-mentioned processing under 4000 rev/mins rotating speed centrifugal 8～12 minutes;
(5) remove high molecular weight protein
Is 10000 daltonian PVC hollow fiber membrane ultrafiltration with Semen sojae atricolor small peptide liquid through the molecular weight that dams, and removes macromolecular albumen;
(6) concentrate
Carry out two and imitate or triple effect concentrates removing Semen sojae atricolor small peptide behind the high molecular weight protein, its volume is concentrated into original 1/2nd to 1/4th;
(7) drying
Spraying drying promptly gets the Semen sojae atricolor small peptide product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009100173781A CN101988080A (en) | 2009-08-01 | 2009-08-01 | Method for producing soybean small peptide |
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|---|---|---|---|
| CN2009100173781A CN101988080A (en) | 2009-08-01 | 2009-08-01 | Method for producing soybean small peptide |
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| CN101988080A true CN101988080A (en) | 2011-03-23 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102251000A (en) * | 2011-06-18 | 2011-11-23 | 唐山师范学院 | Preparation method of compound bioactive peptide |
| CN103555799A (en) * | 2013-10-26 | 2014-02-05 | 吉林大学 | Immunopeptide freeze-dried powder and preparation method thereof |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN107058000A (en) * | 2017-06-30 | 2017-08-18 | 山东覆盆子酒业有限公司 | A kind of raspberry wine with health-care efficacy and preparation method thereof |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN111758881A (en) * | 2020-07-13 | 2020-10-13 | 上海海洋大学 | Landscaping jelly containing soybean small peptides and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN1765921A (en) * | 2005-11-28 | 2006-05-03 | 黄磊 | Separated soybean protein preparation process and prepared soybean small peptide and process |
| CN1935016A (en) * | 2005-09-20 | 2007-03-28 | 中国食品发酵工业研究院 | Industrial production method for high-purity low molecular weight soybean obligopeptide powder |
| CN101457248A (en) * | 2008-11-12 | 2009-06-17 | 广州华宝香精香料有限公司 | Method for preparing maillard flavor peptides by using soja bean separation protein |
-
2009
- 2009-08-01 CN CN2009100173781A patent/CN101988080A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1428431A (en) * | 2001-12-25 | 2003-07-09 | 山东力源集团公司 | Method for preparing soybean polypeptide by utilizing ereyme method |
| CN1935016A (en) * | 2005-09-20 | 2007-03-28 | 中国食品发酵工业研究院 | Industrial production method for high-purity low molecular weight soybean obligopeptide powder |
| CN1765921A (en) * | 2005-11-28 | 2006-05-03 | 黄磊 | Separated soybean protein preparation process and prepared soybean small peptide and process |
| CN101457248A (en) * | 2008-11-12 | 2009-06-17 | 广州华宝香精香料有限公司 | Method for preparing maillard flavor peptides by using soja bean separation protein |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102251000A (en) * | 2011-06-18 | 2011-11-23 | 唐山师范学院 | Preparation method of compound bioactive peptide |
| CN103555799A (en) * | 2013-10-26 | 2014-02-05 | 吉林大学 | Immunopeptide freeze-dried powder and preparation method thereof |
| CN103555799B (en) * | 2013-10-26 | 2015-09-23 | 吉林大学 | A kind of immunocompetence peptide freeze-dried powder and preparation method thereof |
| CN105949274A (en) * | 2016-07-08 | 2016-09-21 | 刘新旗 | Novel soybean peptide separating and refining method |
| CN107058000A (en) * | 2017-06-30 | 2017-08-18 | 山东覆盆子酒业有限公司 | A kind of raspberry wine with health-care efficacy and preparation method thereof |
| CN109864134A (en) * | 2019-03-22 | 2019-06-11 | 长春大学 | Soybean and peanut or single substance are the method that raw material produces small-molecular peptides milk and powder |
| CN111758881A (en) * | 2020-07-13 | 2020-10-13 | 上海海洋大学 | Landscaping jelly containing soybean small peptides and preparation method thereof |
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