CN106578421A - Preparation method of high-quality bean pulp protein peptide - Google Patents
Preparation method of high-quality bean pulp protein peptide Download PDFInfo
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- CN106578421A CN106578421A CN201611168419.3A CN201611168419A CN106578421A CN 106578421 A CN106578421 A CN 106578421A CN 201611168419 A CN201611168419 A CN 201611168419A CN 106578421 A CN106578421 A CN 106578421A
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- bean cake
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Abstract
The invention relates to a preparation method of high-quality bean pulp protein peptide. The preparation method mainly comprises the following steps: pulverizing bean pulp; preparing bean pulp mixed liquid; grinding into thick liquid; carrying out enzymolysis; carrying out pressure filtration; carrying out ultra-filtration; and carrying out spray drying to obtain the bean pulp protein peptide. According to the method, the bean pulp which is low in price and extensive in source is used as a raw material, bean pulp protein is hydrolyzed into perfect small molecule peptide by a segmented enzymolysis method, a ultra-filtration membrane separating technology is combined with a spray drying processing technology, the technological process is simple, the production efficiency is high, the cost is low, and therefore, the high-quality bean pulp protein peptide is suitable for large-scale industrial production. The product prepared in the invention is high in protein content and high in active small molecule peptide (the relative molecular weight is between 180 Da and 1,000 Da), growth of weaned pigs can be promoted remarkably, immunity and disease resistance of piglets are improved, and therefore, the high-quality bean pulp protein peptide is a high-quality functional protein feedstuff.
Description
Technical field
The present invention relates to soybean protein peptide extracts processing technique field, more particularly to a kind of system of high-quality bean cake protein peptide
Preparation Method.
Background technology
Bean cake is a kind of side-product obtained after Semen sojae atricolor extracting bean oil, cheap, and source is relatively wide, and its crude protein content is high
Up to 43～48%, it is one of main protein feedss of animal.But, it has been widely used in animal husbandry and feed manufacturing at present
The bean cake overwhelming majority in industry is high-temperature soybean meal, and its albuminous degeneration is serious, and molecular weight is larger, and dissolubility is poor, while also
Containing certain antinutritional factor, this have impact on the digestibility and feeding value of bean cake to a great extent, limits bean
Use of the dregs of rice in breeding production particularly young stock daily ration.
Bean cake albumen can be made to be broken down into small molecular protein and small peptide using biological enzymolysis technology.Small peptide refers to relative point
Not only there is good dissolubility, anti-gel to be formed for dipeptides of the son amount between 180～1000Da, tripeptides and tetrapeptide, small peptide
The characteristics such as property, low-viscosity, and directly absorb in animal body, the low, bioavailability that consumes energy it is high, while aminoacid can also be promoted
Absorb, improve the absorption rate of protein synthesis speed and mineral, with antioxidation, enhancing human body immunity power, promote to move
The physiological functions such as thing growth.Therefore, the nutritional quality of bean cake albumen is improved using modern biotechnology, exploitation is high additional
Value, the functional bioactive albumen feedstuff of high feeding value, particularly promote young stock growth tool to improve growth of animal performance
There is important meaning.
The content of the invention
The technical problem to be solved in the present invention is to provide a kind of preparation method of high-quality bean cake protein peptide, and the method is with rich
Rich bean cake is raw material, and process is simple, low cost, it is easy to implement, products obtained therefrom protein content are high, and active small peptide content is high, easily
In digesting and absorbing, piglet disease-resistant and anti-stress ability can be significantly improved, promote ablactational baby pig growth, be a kind of high-quality
Functional protein feedstuff.
For achieving the above object, the present invention employs the following technical solutions to realize：A kind of high-quality bean cake protein peptide
Preparation method, comprises the following steps：
（1）Prepare bean cake mixed liquor：Defatted soybean meal is crushed into 80-100 mesh sieves, is added water and is stirred, be configured to protein compression
Spend for 8-12%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 8.0-9.0, Cathepsin B AP is added, 2-3h is digested at a temperature of 52-55 DEG C,
Protease F PC is subsequently adding, is continued enzymolysis 2-3h at a temperature of being kept for 45-48 DEG C, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes,
Bean cake enzymolysis solution is obtained；
（4）Filter pressing：By bean cake enzymolysis solution Jing filter press filter pressings, pressing filtering liquid and filter cake are obtained, filter cake drying can after crushing
Make bean dregs feedstuff；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide powder.
Above-mentioned steps（3）Described Cathepsin B AP is the alkaline protease that fermenting bacillus subtilis are extracted, and enzyme activity is
180000-200000U/ml, Cathepsin B AP addition is the 0.5-0.8% of albumen quality in bean cake serosity；Described protease
Neutral protease of the FPC for aspergillus oryzae fermented extracted, enzyme activity are 280000-300000U/ml, and Protease F PC addition is bean
The 0.1-0.3% of albumen quality in dregs of rice serosity.
Above-mentioned steps（4）The filter cloth mesh number of described filter press is 200 mesh.
Above-mentioned steps（6）The intake air temperature of described spray drying is 200-240 DEG C, and air outlet temperature is 70-90 DEG C.
By implementing above-mentioned technical proposal, the present invention has following beneficial effect：
（1）The method of the present invention using the efficient endo protease extracted from microorganism, using subsection enzymolysis method by bean cake egg
Optimal small-molecular peptides are hydrolyzed in vain, and hydrolysis efficiency is high.
（2）Bean cake albumen peptide product protein content prepared by the present invention more than 72%, active small molecular in its total protein
Peptide（Relative molecular weight is between 180～1000Da）Content up to more than 75%, it is easy to digest and assimilate, biological activity is strong, can show
Writing strengthens pigling immunity and premunition, effectively facilitates piglet growth, and life-time service is had no side effect safely.
（3）Method of the present invention technological process is simple, with short production cycle, and production efficiency is high, low cost, is adapted to scale
Commercial production.
Specific embodiment
With reference to specific embodiment, the present invention is further detailed explanation, but embodiments of the present invention are simultaneously
It is not limited to the scope that this embodiment is represented.
Embodiment 1：
（1）Prepare bean cake mixed liquor：Defatted soybean meal was crushed after 100 mesh sieves, is added water and is stirred, being configured to protein concentration was
8%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 8.2, adds the Cathepsin B AP of albumen quality 0.5% in bean cake serosity,
After 3h is digested at a temperature of 52-55 DEG C, Protease F PC of albumen quality 0.3% in bean cake serosity is added, at a temperature of being kept for 45-48 DEG C
Continue enzymolysis 2h, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes, that is, obtains bean cake enzymolysis solution；
（4）Filter pressing：It is 200 mesh by bean cake enzymolysis solution Jing filter press filter pressings, filter cloth mesh number, obtains pressing filtering liquid and filter cake, filters
Cake drying can be made into bean dregs feedstuff after crushing；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide；It is spray-dried
Intake air temperature is 220 DEG C, and air outlet temperature is 80 DEG C.
Embodiment 2：
（1）Prepare bean cake mixed liquor：Defatted soybean meal was crushed after 80 mesh sieves, is added water and is stirred, being configured to protein concentration was
10%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 8.5, adds the Cathepsin B AP of albumen quality 0.8% in bean cake serosity,
After 2h is digested at a temperature of 52-55 DEG C, Protease F PC of albumen quality 0.1% in bean cake serosity is added, at a temperature of being kept for 45-48 DEG C
Continue enzymolysis 3h, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes, that is, obtains bean cake enzymolysis solution；
（4）Filter pressing：It is 200 mesh by bean cake enzymolysis solution Jing filter press filter pressings, filter cloth mesh number, obtains pressing filtering liquid and filter cake, filters
Cake drying can be made into bean dregs feedstuff after crushing；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide；It is spray-dried
Intake air temperature is 230 DEG C, and air outlet temperature is 85 DEG C.
Embodiment 3：
（1）Prepare bean cake mixed liquor：Defatted soybean meal was crushed after 100 mesh sieves, is added water and is stirred, being configured to protein concentration was
10%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 8.8, adds the Cathepsin B AP of albumen quality 0.6% in bean cake serosity,
Protease F PC of albumen quality 0.2% in bean cake serosity after 2.5h is digested at a temperature of 52-55 DEG C, is added, 45-48 DEG C of temperature is kept
It is lower to continue enzymolysis 2.5h, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes, that is, obtains bean cake enzymolysis solution；
（4）Filter pressing：It is 200 mesh by bean cake enzymolysis solution Jing filter press filter pressings, filter cloth mesh number, obtains pressing filtering liquid and filter cake, filters
Cake drying can be made into bean dregs feedstuff after crushing；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide；It is spray-dried
Intake air temperature is 240 DEG C, and air outlet temperature is 90 DEG C.
Embodiment 4：
（1）Prepare bean cake mixed liquor：Defatted soybean meal was crushed after 80 mesh sieves, is added water and is stirred, being configured to protein concentration was
12%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 9.0, adds the Cathepsin B AP of albumen quality 0.7% in bean cake serosity,
After 3h is digested at a temperature of 52-55 DEG C, Protease F PC of albumen quality 0.3% in bean cake serosity is added, at a temperature of being kept for 45-48 DEG C
Continue enzymolysis 2h, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes, that is, obtains bean cake enzymolysis solution；
（4）Filter pressing：It is 200 mesh by bean cake enzymolysis solution Jing filter press filter pressings, filter cloth mesh number, obtains pressing filtering liquid and filter cake, filters
Cake drying can be made into bean dregs feedstuff after crushing；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide；It is spray-dried
Intake air temperature is 210 DEG C, and air outlet temperature is 70 DEG C.
The product obtained to above-described embodiment carries out the detection of crude protein content, molecular weight distribution and product efficacy.
Crude protein content and molecular weight distribution detection：Crude protein content is determined using GB/T6432-94 methods, protein peptide point
Son amount distribution adopts high-efficient gel filtration chromatography（HPLC）Method is determined, and does reference with known molecular amount protein.
As a result such as table 1：
From table 1 it follows that in product obtained by embodiment crude protein content more than 72%, wherein relative molecular weight exists
The small-molecular peptides component of 180-1000Da accounts for the ratio of gross protein and reaches more than 75%, free amine group of the molecular weight less than 180Da
Sour proportion is within 14%.
Efficacy detection：Du × the length of 35 ages in days × Dasanyuan hybridization piglet 120 is selected, by body weight is close, hereditary basiies
Similar principle, is randomly divided into 3 treatment groups, respectively basal diet group ,+0.8% bean cake protein peptide group of basal diet, basis
+ 1.5% bean cake protein peptide group of daily ration, each treatment group set four repetitions, and each repeats 10 pigs, 30 days experimental periods.Bean cake albumen
Peptide is the product prepared by embodiment 1.
The results are shown in Table 2:
From Table 2, it can be seen that compared with matched group, 0.8%, 1.5% bean cake protein peptide group average daily gain of addition is respectively increased
17.1%, 21.2%, diarrhea rate reduces by 50.2%, 37.8% respectively；Blood total protein, globulin, IgG content are respectively increased
19.2-22.4%, 10.1-19.95%, 28.1-55.4%, bean cake protein peptide prepared by this explanation present invention can significantly increase piglet
Immune function and premunition, promote the growth promoter of piglet, improve weaned piglets.
Claims (4)
1. a kind of preparation method of high-quality bean cake protein peptide, comprises the following steps：
（1）Prepare bean cake mixed liquor：Defatted soybean meal is crushed into 80-100 mesh sieves, is added water and is stirred, be configured to protein compression
Spend for 8-12%（w/w）Bean cake mixed liquor；
（2）Defibrination：By bean cake mixed liquor finely ground machine grinding beating 3min, bean cake serosity is obtained；
（3）Enzymolysis：Bean cake plasm PH value is adjusted to 8.0-9.0, Cathepsin B AP is added, 2-3h is digested at a temperature of 52-55 DEG C,
Protease F PC is subsequently adding, is continued enzymolysis 2-3h at a temperature of being kept for 45-48 DEG C, enzymolysis solution is warming up to into 85 DEG C of enzyme denaturing 15 minutes,
Bean cake enzymolysis solution is obtained；
（4）Filter pressing：By bean cake enzymolysis solution Jing filter press filter pressings, pressing filtering liquid and filter cake are obtained, filter cake drying can after crushing
Make bean dregs feedstuff；
（5）Ultrafiltration：Ultra-filtration membrane device of the filtrate Jing molecular cut offs of collection for 5000Da is carried out into ultrafiltration, permeate is collected；
（6）It is spray-dried：Resulting ultrafiltration permeate is spray-dried, that is, is obtained bean cake protein peptide powder.
2. the preparation method of a kind of high-quality bean cake protein peptide according to claim 1, it is characterised in that：Step（（3）Institute
The Cathepsin B AP for stating is the alkaline protease that fermenting bacillus subtilis are extracted, and enzyme activity is 180000-200000U/ml, protease
BAP additions are the 0.5-0.8% of albumen quality in bean cake serosity；Neutrality of described Protease F PC for aspergillus oryzae fermented extracted
Protease, enzyme activity are 280000-300000U/ml, and Protease F PC addition is the 0.1- of albumen quality in bean cake serosity
0.3%。
3. the preparation method of a kind of high-quality bean cake protein peptide according to claim 1, it is characterised in that：Step（4）Institute
The filter cloth mesh number of the filter press stated is 200 mesh.
4. the preparation method of a kind of high-quality bean cake protein peptide according to claim 1, it is characterised in that：Step（6）Institute
The intake air temperature of the spray drying stated is 200-240 DEG C, and air outlet temperature is 70-90 DEG C.
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| CN201611168419.3A CN106578421A (en) | 2016-12-16 | 2016-12-16 | Preparation method of high-quality bean pulp protein peptide |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109438552A (en) * | 2018-09-17 | 2019-03-08 | 天津科技大学 | A kind of methods and applications preparing defatted soybean meal storage iron polypeptide using protease |
| CN110200127A (en) * | 2019-06-18 | 2019-09-06 | 康地饲料（中国）有限公司 | A kind of function nutrition packet and the preparation method and application thereof promoting sow in lactation fecund milk |
| CN113812507A (en) * | 2021-09-27 | 2021-12-21 | 长春大学 | Method for preparing digestion-free soybean protein by taking high-temperature and low-temperature soybean meal as raw material |
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| CN1489923A (en) * | 2003-09-16 | 2004-04-21 | 北京陶正生物工程技术有限公司 | Method for producing soya protein bioactive peptide |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109438552A (en) * | 2018-09-17 | 2019-03-08 | 天津科技大学 | A kind of methods and applications preparing defatted soybean meal storage iron polypeptide using protease |
| CN109438552B (en) * | 2018-09-17 | 2022-03-25 | 天津科技大学 | Method for preparing defatted soybean meal iron-storing polypeptide by using protease and application |
| CN110200127A (en) * | 2019-06-18 | 2019-09-06 | 康地饲料（中国）有限公司 | A kind of function nutrition packet and the preparation method and application thereof promoting sow in lactation fecund milk |
| CN113812507A (en) * | 2021-09-27 | 2021-12-21 | 长春大学 | Method for preparing digestion-free soybean protein by taking high-temperature and low-temperature soybean meal as raw material |
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Application publication date: 20170426 |