CN106987614B - Method for purifying starch and soluble black rice protein from germinated black rice - Google Patents
Method for purifying starch and soluble black rice protein from germinated black rice Download PDFInfo
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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Abstract
A method for purifying starch and soluble black rice protein from germinated black rice comprises: (1) crushing germinated black rice to prepare black rice milk; (2) sieving to remove rice bran layer; (3) microfluid, strengthening the combination of the dispersed protein and the starch; (4) fermenting to loosen the structure of the protein and the starch; (5) performing enzymolysis reaction to hydrolyze the protein into small molecular peptides; (6) washing with swirling flow to obtain high-purity black rice starch and supernatant containing peptides and active biological elements; (7) the protein which is not completely hydrolyzed is subjected to deamidation modification, so that the solubility is fully improved; (8) neutralizing, desalting, removing ash content in soluble protein, and retaining bioactive elements; (9) spray drying, and embedding gamma-aminobutyric acid, flavone, etc. to obtain soluble black rice protein. The invention has strong practicability, fully utilizes the advantages of the germinated black rice, and simultaneously coproduces protein and starch for the first time in the market of the brown rice at present, thereby having larger market space in the future.
Description
Technical Field
The invention relates to the technical field of food processing, in particular to a method for preparing high-purity black rice starch and high-solubility black rice protein rich in bioactive components by taking germinated black rice as a raw material.
Background
The black rice is a processed product of black rice, belongs to the japonica rice class, and is a special variety formed by long-term cultivation of gramineous plant rice. The rice grains are oblong in appearance, the rice hulls are grey brown, the grain types comprise indica rice and japonica rice, and the black rice is non-glutinous rice. The black rice is black or black brown, has rich nutrition and high food and medicinal value, can be used for making various nutritional foods and wines besides porridge, and is in the reputation of black pearl and the king of world rice. The black rice has more bran layers, less starch content and higher protein content, and the protein and the starch are combined very tightly. Therefore, although the nutrition value is high, the fine processing difficulty is high, the separate purification difficulty of the protein and the starch is extremely high, and particularly, the high-purity starch and the soluble black rice protein are extracted. After the black rice germinates, the black rice has higher nutritive value, higher content of histidine and arginine and higher content of antioxidant flavone; the gamma-aminobutyric acid is rich in gamma-aminobutyric acid, is a non-protein amino acid and has extremely important physiological functions; the bioactive factors are enriched in soluble protein, and have high nutritive value. In addition, in the germination process of the black rice, tissues are softened, the activity of amylase is in a large increasing trend, and the content of starch is in a decreasing trend; this facilitates the separation of starch from protein during subsequent processing.
In the prior art, the black rice is crushed, so that the structure wrapped by protein and starch is difficult to destroy completely; in addition, high-purity black rice starch is difficult to obtain by hydrolyzing starch, and the content of general protein is more than 2%; at present, the soluble black rice protein is impossible to obtain from the phoenix feather unicorn of the domestic black rice starch producing enterprise.
Disclosure of Invention
In view of the above problems with the prior art, the present application provides a method for purifying starch and soluble black rice protein from germinated black rice. The invention has stronger practicability, fully utilizes the advantages of the germinated black rice, and simultaneously, the brown rice co-produces protein and starch for the first time in the market, so that the invention has larger market space in the future.
The technical scheme of the invention is as follows:
a method for purifying starch and soluble black rice protein from germinated black rice, comprising the steps of:
(1) soaking and crushing: pulverizing germinated fructus Zizaniae Caduciflorae with a beater, and circularly reacting for 3-10min with a colloid mill;
(2) screening: sieving the feed liquid obtained in the step (1) through a sieve of 80-150 meshes, and screening and intercepting a rice bran layer to ensure that the weight of undersize products is 2-6% of the weight of the feed liquid obtained in the step (1);
(3) ultramicro jet flow: dispersing the undersize products screened in the step (2) at a high speed by ultramicro jet, wherein the operating pressure is 30-45MPa, and the particle size of the dispersed feed liquid is 0.2-5 mu m;
(4) and (3) standardization: adding water into the feed liquid obtained in the step (3) to adjust the concentration of the substrate to be 5-15%, and adding acid to adjust the pH value to be 2.5-5.5;
(5) fermentation: adding fermentation strains into the black rice pulp obtained after standardization in the step (4), and fermenting for 12-24h at 40-50 ℃;
(6) enzyme reaction: adding compound protease into the fermented feed liquid obtained in the step (5), and stirring and reacting for 2-6h at 40-55 ℃;
(7) and (3) cyclone washing: carrying out cyclone washing on the feed liquid obtained in the step (6) to obtain a cyclone light phase and a cyclone heavy phase;
(8) deamidation: adding 0.2-0.4N hydrochloric acid into the cyclone light phase obtained in the step (7), and stirring and reacting for 2-4h at 75-90 ℃;
(9) plate and frame filter pressing and air flow drying: performing plate-and-frame filter pressing on the rotational flow heavy phase obtained in the step (7), and drying by airflow to obtain black rice starch;
(10) neutralizing: adjusting the pH of the feed liquid obtained in the step (8) to 6.0-6.5 by using potassium hydroxide or sodium hydroxide with the concentration of 4% -10%;
(11) desalting: passing the feed liquid neutralized in the step (10) through a nanofiltration-reverse osmosis combined system to remove ash salts and retain bioactive elements;
(12) spray drying: and (4) carrying out spray drying on the feed liquid obtained in the step (11), and embedding flavone and gamma-aminobutyric acid to obtain soluble black rice protein.
Preferably, the germinated black rice in the step (1) is obtained by intermittently adding water to soak and culture at 22-28 ℃, and germinating in dark, is rich in histidine, arginine, flavone and gamma-aminobutyric acid, belongs to one of brown rice, and comprises black polished round-grained rice and black long-grained nonglutinous rice; after the reaction of the colloid mill, the grain diameter D50 of the feed liquid is 50-150 μm.
Preferably, the acid solution used for adjusting the pH in the step (4) is one or more of hydrochloric acid, acetic acid and phosphoric acid, and the concentration of the acid solution is 1-2 mol/L.
Preferably, the fermentation strain in the step (5) is one or more of lactobacillus, lactobacillus bulgaricus, lactobacillus acidophilus and bifidobacterium; the addition amount of the fermentation strain is 0.1-0.5% of the dry matter.
Preferably, the compound protease in the step (6) is two or more of papain, trypsin, pepsin, bromelain and subtilisin; the addition amount of the compound protease is 2-10 per mill of dry matter; the stirring speed is 60-100 rpm.
Preferably, the cyclone washing in the step (7) is 3-9 stages of step-by-step gradient separation, wherein the ratio of the feeding amount to the washing water flow is 1.2-3.8:1, and the washing pressure is 0.4-1.0 Mpa.
Preferably, the solid content of the cyclone light phase in the step (8) is 0.7% -1.5%; adding hydrochloric acid to the concentration of 0.2-0.4 mol/L; the stirring speed is 120-200 rpm; the deamidation degree of the protein reaches 32-50%.
Preferably, the solid content of the cyclone heavy phase in the step (9) is 10% -15%; the pressure of plate-frame filter pressing is 0.6-1.0 MPa; the air inlet temperature of the airflow drying is 130-150 ℃, the air outlet temperature is 60-70 ℃, and the compressed air is more than 0.5 Mpa.
Preferably, in the step (11), the nanofiltration-reverse osmosis combined membrane separation system is NF-RO, the conductivity of the feed liquid before desalination is 2200-; removing ash salt and retaining bioactive components.
Preferably, in the step (12), the spray drying is performed, the temperature of the material liquid is maintained at 70-85 ℃, the temperature of the inlet air is 180-220 ℃, and the temperature of the outlet air is 80-90 ℃.
The beneficial technical effects of the invention are as follows:
according to the invention, the germinated black rice is innovatively used as a raw material, the structure of protein and starch is thoroughly opened by combining the processes of microjet, fermentation and the like, and the protein and the starch are completely separated and purified by using the technologies of enzymolysis, cyclone washing, membrane and the like, so that the high-purity black rice starch is obtained; by utilizing a spray drying mode, bioactive factors such as flavone, gamma-aminobutyric acid and the like are embedded and perfectly combined with soluble black rice protein.
In order to overcome the technical difficulties of more bran layers of the black rice, less starch content and particularly tight combination of protein and starch, the invention removes the bran layers of the rice by screening and strengthens the combination of the dispersed protein and the starch by microjet. The high-purity black rice starch is obtained by the efficient synergy of enzymolysis and cyclone washing, and the structural damage of the starch in the process of preparing the starch by the traditional hydrolysis method is avoided. The method ensures the solubility of the protein to be improved by a deamidation process, selectively removes ash and the like by combining a membrane technology, and retains soluble protein and bioactive factors. Embedding flavone and gamma-aminobutyric acid enriched in germinated black rice, keeping bioactivity by spray drying, and perfectly combining with soluble protein
The black rice starch prepared by the invention has higher purity, and the protein content is less than or equal to 0.5 percent; the prepared soluble black rice protein is rich in bioactive substances, the content of gamma-aminobutyric acid is 80-90mg/100g, and the content of flavone is 8500-8850 mg/kg; the protein content is up to 40-50%; the protein solubility index (NSI) is more than or equal to 80; the emulsion stability is more than or equal to 88, and the emulsion has better physiological function to human body.
Detailed Description
The present invention will be described in detail with reference to examples.
Example 1:
(1) soaking and crushing: intermittently soaking germinated black long-shaped rice cultured in dark condition at 22 deg.C in water, pulverizing by beater, and circularly reacting for 10min by colloid mill;
(2) screening: passing the feed liquid obtained after the colloid mill circularly reacts through an 80-mesh sieve, and sieving and intercepting a rice bran layer, wherein the undersize is 2% of the raw materials;
(3) ultramicro jet flow: dispersing the sieved undersize through ultramicro jet flow at high speed, wherein the operating pressure is 45 MPa;
(4) and (3) standardization: adding water into the feed liquid obtained after ultramicro jet flow to adjust the concentration of a substrate to 5%, and adjusting the pH value to 5.5 by using 1mol/L hydrochloric acid or acetic acid;
(5) fermentation: adding one or more fermentation strains of lactobacillus, Lactobacillus bulgaricus or Lactobacillus acidophilus 0.1% by weight of the raw materials into the standardized black rice pulp, and fermenting at 50 deg.C for 24 hr;
(6) enzyme reaction: adding two or three of papain, trypsin or pepsin of 2 ‰ of the raw materials into the fermented feed liquid, stirring at 40 deg.C for 6 hr, and stirring at 100 rpm;
(7) and (3) cyclone washing: gradually and gradiently separating the feed liquid after the enzyme reaction, and carrying out cyclone washing to obtain a cyclone light phase and a cyclone heavy phase; wherein the ratio of the feeding amount to the washing water flow is 1.2:1, the washing pressure is 0.4Mpa, and the washing grade is 3 grades;
(8) deamidation: adding 0.2N hydrochloric acid into the swirling light phase, and stirring and reacting for 4 hours at the temperature of 75 ℃; the solid content of the rotational flow light phase is 0.7%; adding hydrochloric acid to the concentration of 0.2 mol/L; the stirring speed is 120 rpm;
(9) plate and frame filter pressing and air flow drying: performing plate-and-frame filter pressing on the rotational flow heavy phase obtained in the step (7), and drying by airflow to obtain black rice starch; wherein the solid content of the rotational flow heavy phase is 10 percent; the pressure of plate-frame filter pressing is 0.6 MPa; the air inlet temperature of the air flow drying is 130 ℃, the air outlet temperature is 60 ℃, and the compressed air is more than 0.5 Mpa.
(10) Neutralizing: adjusting the pH of the feed liquid obtained in the step (8) to 6.0 by using potassium hydroxide with the concentration of 4%;
(11) desalting: removing salts such as ash and the like from the neutralized feed liquid by a nanofiltration-reverse osmosis combined system NF-RO, and reserving bioactive elements; wherein the conductivity of the feed liquid before desalination is 2200 mus/ml;
(12) spray drying: and (3) carrying out spray drying on the desalted feed liquid, and embedding flavone, gamma-aminobutyric acid and the like to obtain the soluble black rice protein. Wherein the temperature of the feed liquid is maintained at 70 ℃, the air inlet temperature of the spray drying is 180 ℃, and the air outlet temperature is 80 ℃.
Example 2:
(1) soaking and crushing: intermittently soaking germinated black long-shaped rice cultured in dark condition at 25 deg.C in water, pulverizing by beater, and circularly reacting for 6min by colloid mill;
(2) screening: passing the feed liquid obtained after the colloid mill circularly reacts through a 120-mesh sieve, and sieving and intercepting a rice bran layer, wherein the undersize is 4% of the raw materials;
(3) ultramicro jet flow: dispersing the sieved undersize through ultramicro jet flow at high speed, wherein the operating pressure is 38 MPa;
(4) and (3) standardization: adding water into the feed liquid obtained after ultramicro jet flow to adjust the concentration of the substrate to 10 percent, and adjusting the pH value to 3.25 by using 1.5mol/L hydrochloric acid or phosphoric acid;
(5) fermentation: adding 0.3% of one or more fermentation strains of lactobacillus, lactobacillus bulgaricus or bifidobacterium according to the raw materials into the standardized black rice pulp, and fermenting at 45 ℃ for 12 h;
(6) enzyme reaction: adding two or three of papain, trypsin or subtilisin of 6 per mill based on the raw materials into the fermented feed liquid, stirring at 48 deg.C for 4h, and stirring at 80 rpm;
(7) and (3) cyclone washing: gradually and gradiently separating the feed liquid after the enzyme reaction, and carrying out cyclone washing to obtain a cyclone light phase and a cyclone heavy phase; wherein the ratio of the feeding amount to the washing water flow is 2.5:1, the washing pressure is 0.7Mpa, and the washing grade is 6 grades;
(8) deamidation: adding 0.3N hydrochloric acid into the swirling light phase, and stirring and reacting for 3 hours at 82 ℃; the solid content of the rotational flow light phase is 1.1%; adding hydrochloric acid to the concentration of 0.3 mol/L; the stirring speed is 160 rpm;
(9) plate and frame filter pressing and air flow drying: performing plate-and-frame filter pressing on the rotational flow heavy phase obtained in the step (7), and drying by airflow to obtain black rice starch; wherein the solid content of the rotational flow heavy phase is 12.5 percent; the pressure of plate-frame filter pressing is 0.8 MPa; the air inlet temperature of the air flow drying is 140 ℃, the air outlet temperature is 65 ℃, and the compressed air is more than 0.5 Mpa.
(10) Neutralizing: adjusting the pH of the feed liquid obtained in the step (8) to 6.25 by using 7% potassium hydroxide;
(11) desalting: removing salts such as ash and the like from the neutralized feed liquid by a nanofiltration-reverse osmosis combined system NF-RO, and reserving bioactive elements; wherein the conductivity of the feed liquid before desalination is 3600 mus/ml;
(12) spray drying: and (3) carrying out spray drying on the desalted feed liquid, and embedding flavone, gamma-aminobutyric acid and the like to obtain the soluble black rice protein. Wherein the temperature of the feed liquid is maintained at 78 ℃, the air inlet temperature of spray drying is 200 ℃, and the air outlet temperature is 85 ℃.
Example 3:
(1) soaking and crushing: intermittently soaking germinated black long-shaped rice cultured in dark condition at 28 deg.C in water, pulverizing by beater, and circularly reacting for 3min by colloid mill;
(2) screening: passing the feed liquid obtained after the colloid mill circularly reacts through a 150-mesh sieve, and sieving and intercepting a rice bran layer, wherein the undersize is 6% of the raw materials;
(3) ultramicro jet flow: dispersing the sieved undersize through ultramicro jet flow at high speed, wherein the operating pressure is 30 MPa;
(4) and (3) standardization: adding water into the feed liquid obtained after ultramicro jet flow to adjust the concentration of a substrate to 15%, and adjusting the pH value to 2.5 by using 2mol/L acetic acid or phosphoric acid;
(5) fermentation: adding 0.5% of one or more fermentation strains of lactobacillus, bifidobacterium or lactobacillus acidophilus into the standardized black rice pulp, and fermenting at 40 deg.C for 18 h;
(6) enzyme reaction: adding two or three of 10 per mill of trypsin, pepsin or subtilisin into the fermented feed liquid, stirring at 55 deg.C for 2 hr, and stirring at 60 rpm;
(7) and (3) cyclone washing: gradually and gradiently separating the feed liquid after the enzyme reaction, and carrying out cyclone washing to obtain a cyclone light phase and a cyclone heavy phase; wherein the ratio of the feeding amount to the washing water flow is 3.8:1, the washing pressure is 1.0Mpa, and the washing grade is 9 grades;
(8) deamidation: adding 0.2N hydrochloric acid into the swirling light phase, and stirring and reacting for 2 hours at 90 ℃; the solid content of the rotational flow light phase is 1.5 percent; adding hydrochloric acid to the concentration of 0.4 mol/L; the stirring speed is 200 rpm;
(9) plate and frame filter pressing and air flow drying: performing plate-and-frame filter pressing on the rotational flow heavy phase obtained in the step (7), and drying by airflow to obtain black rice starch; wherein the solid content of the rotational flow heavy phase is 15 percent; the pressure of plate-frame filter pressing is 1.0 MPa; the inlet air temperature of the air flow drying is 150 ℃, the outlet air temperature is 70 ℃, and the compressed air is more than 0.5 Mpa.
(10) Neutralizing: adjusting the pH of the feed liquid obtained in the step (8) to 6.5 by using sodium hydroxide with the concentration of 4%;
(11) desalting: removing salts such as ash and the like from the neutralized feed liquid by a nanofiltration-reverse osmosis combined system NF-RO, and reserving bioactive elements; wherein the conductivity of the feed liquid before desalination is 5000 mus/ml;
(12) spray drying: and (3) carrying out spray drying on the desalted feed liquid, and embedding flavone, gamma-aminobutyric acid and the like to obtain the soluble black rice protein. Wherein the temperature of the feed liquid is maintained at 85 ℃, the air inlet temperature of the spray drying is 220 ℃, and the air outlet temperature is 90 ℃.
Test example 1:
the feed liquids obtained in the step (1) and the step (3) in the preparation processes of the examples 1 to 3 were tested and compared with the particle size obtained after the existing crushing technology (wet shearing crusher, crushing time 5min), and the particle size results are shown in table 1.
TABLE 1
Test example 2:
the deamidation degree of the deamidated feed liquid obtained in step (8) in the preparation processes of examples 1 to 3 was measured, and the results are shown in table 2.
TABLE 2
| Example 1 | Example 2 | Example 3 | |
| Degree of deamidation (%) | 32.4 | 42.1 | 50.8 |
Test example 3:
the feed liquid obtained after the nanofiltration-reverse osmosis combined system in the step (11) in the preparation processes of examples 1 to 3 was tested, and the conductivity results are shown in table 3.
TABLE 3
| Example 1 | Example 2 | Example 3 | |
| Conductivity (μ s/cm) | 221 | 338 | 446 |
Test example 4:
the protein content of the black rice starch obtained by air-drying in step (9) of the preparation processes of examples 1 to 3 was measured and compared with the protein content of the black rice starch prepared by the prior art (the black rice starch prepared by acid hydrolysis), and the results are shown in table 4.
TABLE 4
Test example 5:
the soluble black rice protein obtained by spray drying in step (12) of the preparation processes of examples 1 to 3 was subjected to protein content, nitrogen solubility index, and emulsion stability tests, and the contents of flavone and γ -aminobutyric acid were measured by inspection, and the results are shown in tables 5 and 6.
TABLE 5
| Protein content (%) | Nitrogen solubility index (%) | Emulsion stability (%) | |
| Example 1 | 40.83 | 80.22 | 88.2 |
| Example 2 | 49.88 | 85.68 | 82.6 |
| Example 3 | 46.21 | 86.04 | 94.0 |
TABLE 6
| Flavone content (mg/kg) | Content of gamma-aminobutyric acid (mg/100g) | |
| Example 1 | 8521 | 80.3 |
| Example 2 | 8724 | 85.6 |
| Example 3 | 8862 | 90.2 |
The specific embodiments described herein are merely illustrative of the spirit and some of the experiments performed. Various modifications or additions may be made or substituted in a similar manner to the specific embodiments described herein by those skilled in the art without departing from the spirit of the invention or exceeding the scope thereof as defined in the appended claims.
Claims (2)
1. A method for purifying starch and soluble black rice protein from germinated black rice, characterized by comprising the steps of:
(1) soaking and crushing: pulverizing germinated fructus Zizaniae Caduciflorae with a beater, and circularly reacting for 3-10min with a colloid mill; the germinated black rice is obtained by intermittently adding water at 22-28 deg.C, soaking and culturing, and germinating in dark, is rich in histidine, arginine, flavone, and gamma-aminobutyric acid, and is one of brown rice, including black polished round-grained rice and black long-grained nonglutinous rice; after the reaction of the colloid mill, the grain diameter D50 of the feed liquid is 50-150 μm;
(2) screening: sieving the feed liquid obtained in the step (1) through a sieve of 80-150 meshes, and screening and intercepting a rice bran layer to ensure that the weight of undersize products is 2-6% of the weight of the feed liquid obtained in the step (1);
(3) ultramicro jet flow: dispersing the undersize products screened in the step (2) at a high speed by ultramicro jet, wherein the operating pressure is 30-45MPa, and the particle size of the dispersed feed liquid is 0.2-5 mu m;
(4) and (3) standardization: adding water into the feed liquid obtained in the step (3) to adjust the concentration of the substrate to be 5-15%, and adding acid to adjust the pH value to be 2.5-5.5;
(5) fermentation: adding fermentation strains into the black rice pulp obtained after standardization in the step (4), and fermenting for 12-24h at 40-50 ℃; the fermentation strain is one or more of lactobacillus bulgaricus, lactobacillus acidophilus and bifidobacterium; the addition amount of the fermentation strain is 0.1 to 0.5 percent of the dry matter;
(6) enzyme reaction: adding compound protease into the fermented feed liquid obtained in the step (5), and stirring and reacting for 2-6h at 40-55 ℃; the compound protease is two or more of papain, trypsin, pepsin and subtilisin; the addition amount of the compound protease is 2-10 per mill of dry matter; the stirring speed is 60-100 rpm;
(7) and (3) cyclone washing: carrying out cyclone washing on the feed liquid obtained in the step (6) to obtain a cyclone light phase and a cyclone heavy phase; the cyclone washing is 3-9 stage-by-stage gradient separation, wherein the ratio of the feeding amount to the washing water flow is 1.2-3.8:1, and the washing pressure is 0.4-1.0 Mpa;
(8) deamidation: adding hydrochloric acid into the rotational flow light phase obtained in the step (7), and stirring and reacting for 2-4h at 75-90 ℃; the solid content of the rotational flow light phase is 0.7-1.5%; adding hydrochloric acid to the concentration of 0.2-0.4 mol/L; the stirring speed is 120-200 rpm; the deamidation degree of the protein reaches 32-50 percent;
(9) plate and frame filter pressing and air flow drying: performing plate-and-frame filter pressing on the rotational flow heavy phase obtained in the step (7), and drying by airflow to obtain black rice starch; the solid content of the rotational flow heavy phase is 10-15%; the pressure of plate-frame filter pressing is 0.6-1.0 MPa; the air inlet temperature of the airflow drying is 130-150 ℃, the air outlet temperature is 60-70 ℃, and the compressed air is more than 0.5 Mpa;
(10) neutralizing: adjusting the pH of the feed liquid obtained in the step (8) to 6.0-6.5 by using potassium hydroxide or sodium hydroxide with the concentration of 4% -10%;
(11) desalting: passing the feed liquid neutralized in the step (10) through a nanofiltration-reverse osmosis combined system to remove ash salts and retain bioactive elements; the nanofiltration-reverse osmosis combined membrane separation system is NF-RO, the conductivity of the feed liquid before desalination is 2200-; removing ash salt and retaining bioactive components;
(12) spray drying: carrying out spray drying on the feed liquid obtained in the step (11), and embedding flavone and gamma-aminobutyric acid to obtain soluble black rice protein; and the spray drying is carried out, wherein the temperature of the material liquid is maintained at 70-85 ℃, the temperature of the inlet air is 180-220 ℃, and the temperature of the outlet air is 80-90 ℃.
2. The method of claim 1, wherein: and (4) adjusting the pH value in the step (4) by using one or more of hydrochloric acid, acetic acid and phosphoric acid, wherein the concentration of the acid solution is 1-2 mol/L.
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| CN103931867A (en) * | 2014-05-08 | 2014-07-23 | 无锡群硕谷唐生物科技有限公司 | Method for coproduction of rice starch and rice protein |
| CN104082673A (en) * | 2014-07-23 | 2014-10-08 | 中恩（天津）营养科技有限公司 | Method for simultaneously preparing low-protein rice and rice immunization peptide |
| CN106071855A (en) * | 2016-06-15 | 2016-11-09 | 中南林业科技大学 | A kind of processing method of the fructus zizaniae caduciflorae and products thereof that germinates |
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| CN102165989A (en) * | 2011-04-12 | 2011-08-31 | 江南大学 | Preparation method of soluble rice protein |
| CN103931867A (en) * | 2014-05-08 | 2014-07-23 | 无锡群硕谷唐生物科技有限公司 | Method for coproduction of rice starch and rice protein |
| CN104082673A (en) * | 2014-07-23 | 2014-10-08 | 中恩（天津）营养科技有限公司 | Method for simultaneously preparing low-protein rice and rice immunization peptide |
| CN106071855A (en) * | 2016-06-15 | 2016-11-09 | 中南林业科技大学 | A kind of processing method of the fructus zizaniae caduciflorae and products thereof that germinates |
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Denomination of invention: A method for purifying starch and soluble black rice protein from sprouted black rice Effective date of registration: 20230828 Granted publication date: 20210219 Pledgee: Agricultural Bank of China Limited by Share Ltd. Wuxi Huishan branch Pledgor: WUXI JINNONG BIOTECHNOLOGY Co.,Ltd. Registration number: Y2023980053750 |