CN1298735C - Process for preparing low-molecular insect protein by pressure hydrolysis of fly maggot protein - Google Patents
Process for preparing low-molecular insect protein by pressure hydrolysis of fly maggot protein Download PDFInfo
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- CN1298735C CN1298735C CNB2005100263830A CN200510026383A CN1298735C CN 1298735 C CN1298735 C CN 1298735C CN B2005100263830 A CNB2005100263830 A CN B2005100263830A CN 200510026383 A CN200510026383 A CN 200510026383A CN 1298735 C CN1298735 C CN 1298735C
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Abstract
The present invention relates to a method for preparing insect protein of low molecular weight by hydrolyzing fly maggot protein in a pressurization mode in the technical field of biological engineering. Fresh fly maggots are used as raw materials; after water washing, homogenate and filtering are carried out, the raw materials are directly heated, pressurized and stirred in a reaction still for hydrolyzation; then, a finished product of protein is obtained by spray drying after self-evaporation of pressure reduction, water-oil separation and the filtering of hydrolysate are carried out. The method of the present invention has the characteristics of a few steps, high utilization ratio of the raw materials and no addition of any additive; the insect protein of low molecular weight is a pure natural product, the quality index reaches an edible level, and the insect protein of low molecular weight can be used as an additive for a nutrient enhancer, a food additive, a health-care food, a culture medium in fermentation industry and other biological products. The present invention has the advantages of simple process, less equipment investment, high yield, no pollution, high utilization ratio of raw materials, low cost, obvious economic benefit, etc.
Description
Technical field
That the present invention relates to is a kind of preparation method of technical field of bioengineering, particularly a kind of the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein.
Background technology
Housefly is a kind of insect that the most of area of China is the most common, quantity is maximum.As culturing development object, at present domestic can mass-producing, mechanize mass production Musca domestica larva (fly maggot).Fly maggot is a kind of high protein, low-fat good animal proteinum.Contain crude protein 15.62% in the bright fly maggot, contain crude protein 59%～65% in the dried maggot powder, fat 2.6%～12%, and contain abundant biologically active substance and nutritive substance, can be used as medicinal.
Find by prior art documents, fly maggot or fly maggot protein matter are applied in bird and aquaculture as a kind of high-quality animal protein feed, but utilize the research of application facet such as fly maggot protein exploitation new resource food, protective foods, functional food additives also few.Fly maggot protein is carried out soda acid to patent CN1297691A or enzymatic treatment prepares fly maggot protolysate, but molecular weight ranges and its biological activity of the protolysate that obtains are not studied, there is complex process in the employed acid-alkali treatment of this method simultaneously, equipment corrosion is serious, shortcomings such as production environment difference; Though enzyme process prepares the protolysate method than acid hydrolyzation mild condition, enzyme costs an arm and a leg, and will inevitably increase product cost.Pressurizing hydrolysis as a kind of preparation method of low molecular weight protein applied to poultry feather and livestock blood protolysate preparation [Zhang Daogang etc. print during chemical industry, 1996,10 (8), 10-13; Zhao Hongyan etc., amino acid and Biological resources, 1997,19 (2), 28-31], but there is the hydrolysis hypertonia in these methods of being reported or still needs to add acid-base method and enzyme process increases shortcomings such as hydrolysis efficiency.
Summary of the invention
The objective of the invention is to overcome deficiency of the prior art, provide a kind of the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein.Make its technology simple, the raw material availability height, the low and yield height of cost, environmental pollution is little simultaneously.
The present invention is achieved by the following technical solutions, and the present invention is a raw material with fresh fly maggot, and after washing, filtering, hydrolysis is stirred in the pressurization of directly heating in reactor, step-down self-evaporatint n. again, oily water separation, and hydrolyzed solution filters the back spraying drying and gets the protein finished product.
Described fly maggot is meant: when treating that fly maggot grows to 3 ages in days, with the ultrasonication of 19～22kHz of 10～100W 1～15 minute; Polypide after handling is continued to cultivate 48～72 hours, during only supply suitable physiological saline and stop for food.
The described pressurized hydrolysis of heating, 150～200 ℃ of hydrolysis temperatures, hydrolysis pressure 0.5～1.5MPa, hydrolysis time 8～16 hours.
The described pressurized hydrolysis of heating, filtrate with the ratio of water is: 1: 1.5～4.
The described pressurized hydrolysis of heating, hydrolysis time 8～16 hours planted agents do not stop to stir.
Described protein, the proteinic molecular weight of its low-molecular insect below 30000 dalton, average out to 10000～20000 dalton.Animal experiment shows that this product has the effect that significantly improves body's immunity.
The protein powder that adopts technology of the present invention to obtain is made into 1% the aqueous solution and carries out safe toxicological evaluation test by the GB15193-94 relevant regulations, and sample is to the acute oral LD of male and female small white mouse
50All,, belong to the nontoxicity material with acute toxicity medium lethal dose toxicity grading greater than 21500mg/kg; Particulate experimental result feminine gender; Teratospermia experimental result feminine gender.
The inventive method step is few, and the raw material availability height does not add any additives, is all-natural product, and quality index reaches food grade, can be used as the additive of nutrition-fortifying agent, foodstuff additive, protective foods, fermentation industry substratum and other biological product.The present invention compared with the prior art, hydrolytic process is simple, hydrolytic process is not added any catalyzer, additive; The static oily water separation of hydrolyzed solution, spraying drying get final product product.Thereby the present invention has, and technology is simple, facility investment is few, yield is high, pollution-free, advantages such as raw material availability is high, cost is low, remarkable in economical benefits.
Press GB4789-94 hygiology index to coliform, pathogenic bacterium, total plate count check, total plate count is less than 1000/gram, and coliform is less than 90/100 grams, and pathogenic bacterium do not detect, and meet the food-grade microorganisms index.The heavy metal index of sample is qualified, so product of the present invention can be used as food grade.
The protein powder that adopts technology of the present invention to obtain is made into 1% the aqueous solution, and look light is, transparent, the nothing precipitation; Salmonellas, intestinal bacteria, faecalis upgrowth situation on the nutrient agar plate that this protein powder is joined is good, illustrates that product of the present invention can be used as microbial culture and uses.The present invention can make protein powder 191～252g with the bright fly maggot of per kilogram, and its aminoacids content is as shown in table 1.
The aminoacids content (g/100g) of table 1 highly pressured hydrolysis protein powder
| Amino acid | Content | Amino acid | Content |
| Aspartic acid | 7.06～8.05 | Methionine(Met) | 2.17～2.69 |
| Threonine | 2.94～3.39 | Isoleucine | 3.83～4.40 |
| Serine | 3.21～3.68 | Tyrosine | 5.11～5.92 |
| L-glutamic acid | 10.35～12.24 | Phenylalanine | 4.78～5.64 |
| Proline(Pro) | 2.21～2.65 | Methionin | 4.13～5.23 |
| Glycine | 2.67～3.07 | Arginine | 3.17～4.06 |
| L-Ala | 3.45～3.98 | Histidine | 1.84～2.55 |
| Gelucystine | 0.57～1.01 | Tryptophane | - |
| Xie Ansuan | 3.37～3.88 |
Embodiment
Below in conjunction with embodiment the present invention is elaborated.
Embodiment 1:
Artificial Musca domestica larva of being cultured, when treating that fly maggot grows to 3 ages in days, the ultrasonication of 19～22kHz of usefulness 100W 1 minute; Polypide after handling is continued to cultivate 48 hours, during only supply suitable physiological saline and stop for food.Get the 2kg Musca domestica larva, through washing, homogenate is ground with colloidal mill in the sterilization back, collect the fly maggot slurries after grinding, with centrifugal separation filter residue is separated with filtrate, filter residue can be used for chitin extraction, and filtrate is packed in the hydrolysis kettle, adds suitable water, the weight ratio that makes filtrate and water is 1/3, sealing equipment is also opened and is stirred, and rotating speed is 170 rev/mins, is warming up to 175 ℃ naturally, red-tape operati pressure is 0.8MPa, keep directly putting to normal pressure oily water separation after the hydrolyzed solution self-evaporatint n. after 12 hours, oil phase can be used for extracting lipid acid, and the water spraying drying gets the beige pressed powder.The protein molecular weight of gained is less than 30000 dalton, and molecular-weight average is 10000-20000, and this example can get protein powder 425g.
Embodiment 2
Artificial Musca domestica larva of being cultured, when treating that fly maggot grows to 3 ages in days, the ultrasonication of the 19-22kHz of usefulness 10W 15 minutes; Polypide after handling is continued to cultivate 72 hours, during only supply suitable physiological saline and stop for food.Get the 2kg Musca domestica larva, after washing, sterilization, grind homogenate with colloidal mill, collect the fly maggot slurries after grinding, with centrifugal separation filter residue is separated with filtrate, filtrate is packed in the hydrolysis kettle, adds suitable water, and the weight ratio that makes filtrate and water is 1/4, sealing equipment is also opened and is stirred, rotating speed is 180 rev/mins, is warming up to 180 ℃ naturally, under this temperature, red-tape operati pressure is 0.85MPa, keep directly putting to normal pressure oily water separation after the hydrolyzed solution self-evaporatint n. after 10 hours, oil phase can be used for extracting lipid acid, and the water spraying drying gets the beige pressed powder.The protein molecular weight of gained is less than 30000 dalton, and molecular-weight average is 10000-20000, and this example can get protein powder 432g.
Embodiment 3
Artificial Musca domestica larva of being cultured, when treating that fly maggot grows to 3 ages in days, the ultrasonication of the 19-22kHz of usefulness 50W 8 minutes; Polypide after handling is continued to cultivate 60 hours, during only supply suitable physiological saline and stop for food.Get the 3kg Musca domestica larva, through washing, homogenate is ground with colloidal mill in the sterilization back, collect the fly maggot slurries after grinding, with centrifugal separation filter residue is separated with filtrate, filter residue can be used for chitin extraction, and filtrate is packed in the hydrolysis kettle, add suitable water, the weight ratio that makes filtrate and water is 1/2, and sealing equipment is also opened and stirred, and rotating speed is 190 rev/mins, naturally be warming up to 150 ℃, under this temperature, red-tape operati pressure is 0.7MPa, keeps after 11 hours, directly put to normal pressure, oily water separation after the hydrolyzed solution self-evaporatint n., oil phase can be used for extracting lipid acid, and the water spraying drying gets the beige pressed powder.The protein molecular weight of gained is less than 30000 dalton, and molecular-weight average is 12000-20000, and this example can get protein powder 650g.
Claims (5)
1, a kind of the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein, it is characterized in that, fresh fly maggot length is during to 3 ages in days, ultrasonication with 19～22kHz of 10～100W continues after 1～15 minute to cultivate 48～72 hours, only supply suitable physiological saline during this time and stop to supply food, after washing, homogenate, filtration, hydrolysis is stirred in directly heating in reactor pressurization then, filtrate with the ratio of water is: 1: 1.5～4, and 150～200 ℃ of hydrolysis temperatures; Hydrolysis pressure 0.5～1.5MPa; Hydrolysis time 8～16 hours, step-down self-evaporatint n. again, oily water separation, it is the following protein finished products of 30000 dalton that hydrolyzed solution filtration back spraying drying obtains molecular weight.
2, according to claim 1 the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein, it is characterized in that the proteinic molecular-weight average of described low-molecular insect is 10000-20000.
3, according to claim 1 the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein, it is characterized in that, described fly maggot length is during to 3 ages in days, with the ultrasonication of the 19-22kHz of 100W 1 minute, the polypide after handling continued to cultivate 48 hours.
4, according to claim 1 the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein, it is characterized in that, described fly maggot length is during to 3 ages in days, with the ultrasonication of the 19-22kHz of 10W 15 minutes, the polypide after handling continued to cultivate 72 hours.
5, according to claim 1 the fly maggot protein pressurized hydrolysis is prepared the low-molecular insect method of protein, it is characterized in that, described fly maggot length is during to 3 ages in days, with the ultrasonication of the 19-22kHz of 50W 8 minutes, the polypide after handling continued to cultivate 60 hours.
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| Application Number | Priority Date | Filing Date | Title |
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| CNB2005100263830A CN1298735C (en) | 2005-06-02 | 2005-06-02 | Process for preparing low-molecular insect protein by pressure hydrolysis of fly maggot protein |
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| CNB2005100263830A CN1298735C (en) | 2005-06-02 | 2005-06-02 | Process for preparing low-molecular insect protein by pressure hydrolysis of fly maggot protein |
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| CN1699405A CN1699405A (en) | 2005-11-23 |
| CN1298735C true CN1298735C (en) | 2007-02-07 |
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Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829151B (en) * | 2010-05-11 | 2012-06-06 | 广东药学院 | Preparation method of fly micromolecular active substance with blood fat-reducing function |
| CN101991608B (en) * | 2010-11-19 | 2012-06-06 | 浙江省医学科学院 | Fly maggot extractive as well as preparation method and application thereof |
| CN102578361B (en) * | 2012-03-28 | 2013-09-11 | 常熟市汇康食品厂 | Maggot compound protein powder |
| CN110683913A (en) * | 2019-10-21 | 2020-01-14 | 武汉楚强生物科技有限公司 | Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof |
| CN112970937A (en) * | 2021-03-26 | 2021-06-18 | 刘文治 | Method for preparing feed additive by cracking insect pulp and other animal and vegetable proteins |
| CN116924837A (en) * | 2023-06-05 | 2023-10-24 | 华南农业大学 | Indigestible nitrogen-rich waste water thermal depolymerization method and plant protection liquid prepared by same |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1397191A (en) * | 2002-01-10 | 2003-02-19 | 司忠 | Process for preparing protein from aseptic fly maggot |
| CN1415757A (en) * | 2002-11-20 | 2003-05-07 | 江南大学 | Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297691A (en) * | 1999-12-01 | 2001-06-06 | 中国科学院动物研究所 | Separation and extraction process of effective component in resource insect |
| CN1397191A (en) * | 2002-01-10 | 2003-02-19 | 司忠 | Process for preparing protein from aseptic fly maggot |
| CN1415757A (en) * | 2002-11-20 | 2003-05-07 | 江南大学 | Method for extracting protein and chitin from fly maggot by using enzyme hydrolysis as well as preparing chitosan from chitin |
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