CN110683913A - Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof - Google Patents

Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof Download PDF

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CN110683913A
CN110683913A CN201910999778.0A CN201910999778A CN110683913A CN 110683913 A CN110683913 A CN 110683913A CN 201910999778 A CN201910999778 A CN 201910999778A CN 110683913 A CN110683913 A CN 110683913A
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insect
hydrolysate
residues
virus
amino acid
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尹涛
陈娇
林春鸿
高琪
吕惠红
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WUHAN CHUQIANG BIOLOGICAL TECHNOLOGY Co Ltd
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WUHAN CHUQIANG BIOLOGICAL TECHNOLOGY Co Ltd
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05BPHOSPHATIC FERTILISERS
    • C05B7/00Fertilisers based essentially on alkali or ammonium orthophosphates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C9/00Fertilisers containing urea or urea compounds

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Abstract

The invention provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following components in percentage by mass: 5-30% of virus-carrying insect residue hydrolysate, 30-60% of amino acid stock solution, 0-30% of urea, 0-15% of phosphate, 0-25% of potassium salt and 5-15% of medium trace elements. The invention takes insect residues with viruses as raw materials to hydrolyze, hydrolysate is chelated with trace elements after being filtered, and other raw materials are gradually added to prepare the water-soluble fertilizer containing amino acid. The insect residues with the viruses contain insect targeted viruses, can form part of specific amino acids after hydrolysis, and meanwhile, the insect residues contain rich amino oligosaccharides, polypeptides, monosaccharides, polysaccharides and other nutrient substances after hydrolysis, so that the growth of crops and the absorption of nutrients can be promoted, and the yield and the quality of the crops are effectively improved.

Description

Amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and preparation method thereof
Technical Field
The invention belongs to the technical field of agricultural fertilizers, and particularly relates to an amino acid-containing fertilizer based on virus-containing insect residue hydrolysate and a preparation method thereof.
Background
At present, insect viruses are a mature technology applied to the field of plant protection, and have the characteristics of high targeting property, strong infectivity in insect groups, environmental friendliness and the like, so that the insect viruses play an important role in a green ecological prevention and control system of agricultural insect pests, and along with the increasing aggravation of international environmental problems and food safety problems in recent years, the application of the insect viruses is greatly promoted, and the insect viruses are gradually becoming an important means for preventing and controlling the ecological agricultural insect pests.
After insect viruses are separated and purified in industrial production, a large number of insect disease residues are left, a large number of virus bodies still exist in the insect disease residues, specific amino acids contained in the virus bodies have a special regulation and control effect on the growth of crops, the content of the specific amino acids in the environment is extremely low, and the amino acids generated after virus proteins are hydrolyzed are applied to the crops or soil, so that the content of free amino acids in the soil can be increased, the growth promotion effect on the crops is realized, the types of the free amino acids in the soil can be enriched, and more regulation and control effects on the crops are realized.
The insect residues are mostly insect tissues containing a large amount of chitin, protein or fat, and are good organic nutrition sources for soil and crops, the chitin can form amino oligosaccharide substances after being decomposed, the amino oligosaccharide substances have important effects on controlling the growth and resistance gene expression of the crops, amino acid or polypeptide formed after the protein is decomposed can be utilized by the crops or soil microorganisms, and the fat is decomposed to form various monosaccharides or polysaccharides which can be used as carbon sources of rhizosphere microorganisms and can also be directly absorbed and utilized by crop roots.
In addition, the insect residues with viruses are main wastes in the insect virus production process, have special unpleasant odor, and can influence the living environment of people on one hand and increase the burden of waste treatment on the other hand if the insect residues are not treated. However, at present, there are few patents for recycling the insect disease residues in the domestic virus industry to produce fertilizers, and mainly because there are few institutions for related research in the domestic insect virus industry, there has not been any deep research on the influence mechanism of specific amino acids constituting insect viruses on crop growth.
Disclosure of Invention
The invention aims to solve the problems of environmental pollution caused by the waste of the existing insect residues with viruses and resource waste caused by the increase of waste treatment burden.
Therefore, the invention provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following components in percentage by mass: 5-30% of virus-carrying insect residue hydrolysate, 30-60% of amino acid stock solution, 0-30% of urea, 0-15% of phosphate, 0-25% of potassium salt and 5-15% of medium trace elements.
Further, the virus-carrying insect residue hydrolysate is prepared by taking virus-carrying insect residues as raw materials, sequentially crushing and centrifuging, separating virus-carrying insect residue solid matters, and performing acidic or alkaline hydrolysis.
Further, the virus-bearing insect residues are at least one of Blattaria insect residues, Lepidoptera insect residues and coleoptera insect residues; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.
Further, the amino acid stock solution contains at least one of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine and glutamic acid, and the mass content of free amino acid is 12-40%.
Further, the phosphate comprises at least one of monoammonium phosphate, diammonium phosphate, monopotassium phosphate and dipotassium phosphate.
Further, the potassium salt includes at least one of potassium chloride, potassium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, and potassium nitrate.
Further, the medium trace elements comprise at least two of ammonium calcium nitrate, calcium chloride, chelated calcium, anhydrous magnesium sulfate, magnesium sulfate heptahydrate, chelated magnesium, zinc sulfate monohydrate, chelated zinc, boric acid, sodium tetraborate decahydrate, sodium octaborate tetrahydrate, ferrous sulfate heptahydrate, chelated iron, copper sulfate pentahydrate, chelated copper, manganese sulfate monohydrate and chelated manganese.
In addition, the invention also provides a preparation method of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate, which comprises the following steps:
1) primarily crushing phosphate, sylvite and medium trace elements to form uniform powder;
2) filtering the hydrolysate of the residual body of the insect with the virus to remove impurities and insoluble substances to form clear and uniform liquid, and premixing the filtered hydrolysate of the residual body of the insect with the virus and the amino acid stock solution according to a proportion to form uniform liquid;
3) putting the powder of urea, phosphate, sylvite and medium trace elements in the step 1) into the mixed solution in the step 2) in proportion, and stirring for dissolving;
4) filtering the reaction liquid completely dissolved in the step 3) to remove insoluble substances and impurities, wherein the filtrate is the amino acid-containing fertilizer.
Further, the granularity of the powder in the step 1) is more than or equal to 60 meshes.
Further, the dissolving temperature in the step 3) is 40-50 ℃, and the dissolving time is 20-160 min.
Compared with the prior art, the invention has the beneficial effects that:
(1) according to the fertilizer containing amino acid and based on the virus-containing insect residue hydrolysate, provided by the invention, according to the theories of plant nutrition and plant physiology, macroelements and medium trace elements required by plant growth are reasonably matched, and are matched with various amino acids necessary for plant growth, and then matched with specific amino acid formed after insect virus hydrolysis and saccharide formed after insect residue hydrolysis, the crop growth can be comprehensively regulated, the comprehensive regulation of various exogenous amino acids can be realized, the absorption and transformation of the crop on the nutrient elements can be improved, and the crop yield can be effectively improved; meanwhile, insect residues with viruses are hydrolyzed to form a plurality of specific amino acids and sugars, and the amino acids and the sugars can be directly absorbed and utilized by crops to promote the secondary metabolism of the crops and form a plurality of secondary metabolites including cellulose, sugars, starch and the like, so that the quality of the crops can be effectively improved.
(2) The amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate provided by the invention takes the virus-containing insect residues as raw materials, and the insect viruses have the characteristics of high targeting property, strong infectivity in insect groups, environmental friendliness and the like, are non-pathogenic to organisms outside the targeting property, and have extremely high safety to crops; the amino acid generated by hydrolyzing the virus protein is applied to the crops or soil, so that the content of free amino acid in the soil can be increased, the variety of free amino acid in the soil can be enriched, and more regulation and control effects on the crops can be realized; meanwhile, most insect residues are insect tissues containing a large amount of chitin, protein or fat, the insect residues are good organic nutrition sources for soil and crops, chitin can form amino oligosaccharide substances after decomposition, the amino oligosaccharide substances have important effects on controlling crop growth and resistance gene expression, amino acid or polypeptide formed after decomposition of protein can be utilized by crops or soil microorganisms, fat is decomposed to form various monosaccharides or polysaccharides which can be used as carbon sources of rhizosphere microorganisms and can also be directly absorbed and utilized by crop root systems.
(3) The preparation method of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate is simple to operate, and overcomes the defects that excessive insoluble substances remain after hydrolysis due to incomplete hydrolysis of insect residues and valence changes occur when trace element ferrous ions and amino acids coexist.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1:
the embodiment provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following raw material components: 25% of virus-bearing insect residue hydrolysate, 40% of amino acid stock solution, 18% of urea, 7% of monopotassium phosphate, 3% of potassium sulfate, 1% of anhydrous magnesium sulfate, 2.5% of zinc sulfate monohydrate, 1.8% of boric acid, 1.5% of chelated iron and 0.2% of blue vitriod. Wherein the total content of free amino acids in the amino acid stock solution accounts for 18% of the total amount of the amino acid stock solution, and the free amino acids comprise 18 kinds of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine and glutamic acid.
The virus-carrying insect residue hydrolysate is prepared by taking virus-carrying insect residues as raw materials, sequentially smashing and centrifuging, separating virus-carrying insect residue solid matters, and performing acidic or alkaline hydrolysis. The virus-carrying insect residue is at least one of Blattaria insect disease residue, Lepidoptera insect disease residue, and Coleoptera insect disease residue; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.
The preparation process of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate comprises the following steps:
(1) crushing: primarily crushing potassium dihydrogen phosphate, potassium nitrate, zinc sulfate monohydrate and citric acid to obtain uniform powder with fineness not less than 60 meshes.
(2) Filtering hydrolysate of residual bodies of insects with viruses: the virus-contained insect residue hydrolysate is filtered by a plate-and-frame filter to remove impurities and insoluble substances, and a clear and uniform liquid is formed.
(3) Premixing liquid raw materials: premixing the filtered residual body hydrolysate with the virus and the amino acid stock solution in a reaction kettle according to a proportion to form uniform liquid.
(4) Dissolving: adding urea, potassium dihydrogen phosphate, potassium nitrate, zinc sulfate monohydrate, boric acid, chelated iron and citric acid into the mixed solution in proportion, and stirring and dissolving in a reaction kettle for 60min at the temperature of 45 ℃.
(5) And (3) filtering: and filtering the completely dissolved reaction solution by a plate-frame filter to remove insoluble substances and impurities, and separating filtrate to obtain the amino acid-containing fertilizer.
Example 2:
the embodiment provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following raw material components: 30% of virus-containing insect residue hydrolysate, 30% of amino acid stock solution, 15% of monopotassium phosphate, 10% of potassium nitrate, 10% of anhydrous magnesium sulfate, 1.5% of zinc sulfate monohydrate, 2% of boric acid and 1.5% of chelated iron. Wherein the total content of free amino acids in the amino acid stock solution accounts for 20% of the total amount of the amino acid stock solution, and the free amino acids comprise 18 kinds of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine and glutamic acid.
The virus-carrying insect residue hydrolysate is prepared by taking virus-carrying insect residues as raw materials, sequentially smashing and centrifuging, separating virus-carrying insect residue solid matters, and performing acidic or alkaline hydrolysis. The virus-carrying insect residue is at least one of Blattaria insect disease residue, Lepidoptera insect disease residue, and Coleoptera insect disease residue; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.
The preparation process of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate comprises the following steps:
(1) crushing: primarily crushing the monopotassium phosphate to obtain uniform powder with fineness not less than 60 meshes.
(2) Filtering hydrolysate of residual bodies of insects with viruses: the virus-contained insect residue hydrolysate is filtered by a plate-and-frame filter to remove impurities and insoluble substances, and a clear and uniform liquid is formed.
(3) Premixing liquid raw materials: premixing the filtered residual body hydrolysate with the virus and the amino acid stock solution in a reaction kettle according to a proportion to form uniform liquid.
(4) Dissolving: adding urea, potassium dihydrogen phosphate, potassium sulfate and boric acid into the mixed solution in proportion, stirring and dissolving in a reaction kettle for 60min, wherein the temperature in the dissolving process is 40 ℃.
(5) And (3) filtering: and filtering the completely dissolved reaction solution by a plate-frame filter to remove insoluble substances and impurities, and separating filtrate to obtain the amino acid-containing fertilizer.
Example 3:
the embodiment provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following raw material components: 5% of virus-carrying insect residue hydrolysate, 60% of amino acid stock solution, 30% of urea, 2.5% of zinc sulfate monohydrate, 1.8% of boric acid, 1.5% of chelated iron and 0.2% of blue vitriol. Wherein the total content of free amino acids in the amino acid stock solution accounts for 20% of the total amount of the amino acid stock solution, and the free amino acids comprise 18 kinds of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine and glutamic acid.
The virus-carrying insect residue hydrolysate is prepared by taking virus-carrying insect residues as raw materials, sequentially smashing and centrifuging, separating virus-carrying insect residue solid matters, and performing acidic or alkaline hydrolysis. The virus-carrying insect residue is at least one of Blattaria insect disease residue, Lepidoptera insect disease residue, and Coleoptera insect disease residue; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.
The preparation process of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate comprises the following steps:
(1) crushing: primarily crushing potassium dihydrogen phosphate, zinc sulfate monohydrate, copper sulfate pentahydrate and citric acid to obtain uniform powder with fineness not less than 60 meshes.
(2) Filtering hydrolysate of residual bodies of insects with viruses: the virus-contained insect residue hydrolysate is filtered by a plate-and-frame filter to remove impurities and insoluble substances, and a clear and uniform liquid is formed.
(3) Premixing liquid raw materials: premixing the filtered residual body hydrolysate with the virus and the amino acid stock solution in a reaction kettle according to a proportion to form uniform liquid.
(4) Dissolving: adding urea, potassium dihydrogen phosphate, potassium sulfate, zinc sulfate monohydrate, boric acid, chelated iron, copper sulfate pentahydrate and citric acid into the mixed solution in proportion, stirring and dissolving in a reaction kettle for 60min, wherein the temperature in the dissolving process is 50 ℃.
(5) And (3) filtering: and filtering the completely dissolved reaction solution by a plate-frame filter to remove insoluble substances and impurities, and separating filtrate to obtain the amino acid-containing fertilizer.
Example 4:
the embodiment provides an amino acid-containing fertilizer based on hydrolysate of residues of insects with viruses, which comprises the following raw material components: 20% of virus-carrying insect residue hydrolysate, 45% of amino acid stock solution, 20% of urea, 7% of monopotassium phosphate, 3% of anhydrous magnesium sulfate, 2.8% of zinc sulfate monohydrate, 1.0% of boric acid, 1.0% of chelated iron and 0.2% of copper sulfate pentahydrate. Wherein the total content of free amino acids in the amino acid stock solution accounts for 20% of the total amount of the amino acid stock solution, and the free amino acids comprise 18 kinds of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine and glutamic acid.
The virus-carrying insect residue hydrolysate is prepared by taking virus-carrying insect residues as raw materials, sequentially smashing and centrifuging, separating virus-carrying insect residue solid matters, and performing acidic or alkaline hydrolysis. The virus-carrying insect residue is at least one of Blattaria insect disease residue, Lepidoptera insect disease residue, and Coleoptera insect disease residue; wherein the insect disease residues of Blattaria include periplaneta americana disease residues, Blattella germanica disease residues, Termite disease residues, and the like, the lepidoptera insect disease residues include Autographa californica disease residues, beet armyworm disease residues, prodenia litura disease residues, cabbage caterpillar disease residues, pine moth disease residues, cotton bollworm disease residues, tea geometrid disease residues, and coleoptera insect disease residues include Typha angustifolia disease residues, scarab beetle disease residues, and the like.
The preparation process of the amino acid-containing fertilizer based on the virus-containing insect residue hydrolysate comprises the following steps:
(1) crushing: primarily crushing potassium dihydrogen phosphate, zinc sulfate monohydrate, copper sulfate pentahydrate and citric acid to obtain uniform powder with fineness not less than 60 meshes.
(2) Filtering hydrolysate of residual bodies of insects with viruses: the virus-contained insect residue hydrolysate is filtered by a plate-and-frame filter to remove impurities and insoluble substances, and a clear and uniform liquid is formed.
(3) Premixing liquid raw materials: premixing the filtered residual body hydrolysate with the virus and the amino acid stock solution in a reaction kettle according to a proportion to form uniform liquid.
(4) Dissolving: adding urea, potassium dihydrogen phosphate, potassium sulfate, zinc sulfate monohydrate, boric acid, chelated iron, copper sulfate pentahydrate and citric acid into the mixed solution in proportion, stirring and dissolving in a reaction kettle for 60min, wherein the temperature in the dissolving process is 50 ℃.
(5) And (3) filtering: and filtering the completely dissolved reaction solution by a plate-frame filter to remove insoluble substances and impurities, and separating filtrate to obtain the amino acid-containing fertilizer.
Application example:
the fertilizer containing amino acid prepared by the invention is applied to potted quick-boiled vegetables by flushing and topdressing, and experiments are carried out in 7 months and 1 day in 2019 in HubeiThe method is carried out in laboratories of Wuhan Chu intensive Biotechnology Limited company in Chua Dian region of Wuhan City, province, and the liquid fertilizer containing amino acid prepared in example 1 is used for flushing and topdressing, the potting matrix is vegetable garden sandy loam, 3 plants are planted in each pot, topdressing is carried out once every 6 days for 4 times, harvesting is carried out after 30 days, and the dry weight of the overground part, the dry weight of the underground part, the soluble sugar content and nitrate Nitrogen (NO) of the test work are measured3 -) Content, and analyzing the difference significance of the results by SPSS software, wherein the analysis results are shown in Table 1.
Table 1: influence of amino acid-containing fertilizer on yield and quality of fast food
Figure 607078DEST_PATH_IMAGE001
Note: the statistical analysis results are the significance of the differences in data between treatments at the level of P < 0.05.
As can be seen from table 1, compared with the application of the compound fertilizer, the dry weight of the overground part and the dry weight of the underground part are both obviously increased after the liquid amino acid-containing fertilizer produced by using the hydrolysate of the residues of the insects with the viruses as the raw material is applied, which indicates that the yield of the fast food can be obviously increased after the amino acid-containing fertilizer is applied for topdressing; compared with the application of the compound fertilizer, after the liquid amino acid-containing fertilizer produced by taking the virus-containing insect residue hydrolysate as the raw material is applied, the soluble sugar content is obviously increased, and the nitrate nitrogen content is obviously reduced, which shows that after the amino acid-containing fertilizer is applied for topdressing, the soluble sugar content of fast vegetables can be obviously increased, and the nitrate nitrogen content of the fast vegetables can be reduced.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.

Claims (10)

1. The fertilizer containing amino acid based on the hydrolysate of the residues of the insects with the viruses is characterized by comprising the following components in percentage by mass: 5-30% of virus-carrying insect residue hydrolysate, 30-60% of amino acid stock solution, 0-30% of urea, 0-15% of phosphate, 0-25% of potassium salt and 5-15% of medium trace elements.
2. The fertilizer containing amino acid and based on the hydrolysate of insect residues with virus of claim 1, wherein the hydrolysate of insect residues with virus is prepared by using insect residues with virus as raw materials, sequentially breaking, centrifuging, separating solid matters of insect residues with virus, and performing acidic or alkaline hydrolysis.
3. The amino acid-containing fertilizer based on the virus-carrying insect residue hydrolysate according to claim 2, wherein the virus-carrying insect residue is at least one of a Blattaria insect disease residue, a Lepidoptera insect disease residue, and a Coleoptera insect disease residue.
4. The fertilizer containing amino acids based on the hydrolysate of residues of viral insects as claimed in claim 1, wherein the amino acid stock solution comprises at least one of leucine, proline, isoleucine, phenylalanine, lysine, threonine, methionine, tryptophan, histidine, tyrosine, valine, alanine, serine, glycine, arginine, aspartic acid, cysteine, glutamic acid, and the content of free amino acids is 12-40% by mass.
5. The amino acid-containing fertilizer based on the hydrolysate of residues of viral insects of claim 1, wherein said phosphate comprises at least one of monoammonium phosphate, diammonium phosphate, monopotassium phosphate, and dipotassium phosphate.
6. The fertilizer containing amino acids based on the hydrolysate of viral insect residues according to claim 1, wherein the potassium salt comprises at least one of potassium chloride, potassium sulfate, potassium dihydrogen phosphate, dipotassium hydrogen phosphate, and potassium nitrate.
7. The amino acid-containing fertilizer based on viral insect residue hydrolysate of claim 1, wherein the medium trace elements comprise at least two of calcium ammonium nitrate, calcium chloride, chelated calcium, anhydrous magnesium sulfate, magnesium sulfate heptahydrate, chelated magnesium, zinc sulfate monohydrate, chelated zinc, boric acid, sodium tetraborate decahydrate, sodium octaborate tetrahydrate, ferrous sulfate heptahydrate, chelated iron, copper sulfate pentahydrate, chelated copper, manganese sulfate monohydrate, and chelated manganese.
8. The method for preparing the fertilizer containing amino acids based on the virus-containing insect residue hydrolysate according to any one of claims 1 to 7, comprising the following steps:
1) primarily crushing phosphate, sylvite and medium trace elements to form uniform powder;
2) filtering the hydrolysate of the residual body of the insect with the virus to remove impurities and insoluble substances to form clear and uniform liquid, and premixing the filtered hydrolysate of the residual body of the insect with the virus and the amino acid stock solution according to a proportion to form uniform liquid;
3) putting the powder of urea, phosphate, sylvite and medium trace elements in the step 1) into the mixed solution in the step 2) in proportion, and stirring for dissolving;
4) filtering the reaction liquid completely dissolved in the step 3) to remove insoluble substances and impurities, wherein the filtrate is the amino acid-containing fertilizer.
9. The method for preparing the fertilizer containing amino acid based on the virus-containing insect residue hydrolysate of claim 8, wherein the particle size of the powder in the step 1) is not less than 60 meshes.
10. The method for preparing the fertilizer containing amino acids based on the hydrolysate of residues of insects with viruses as claimed in claim 8, wherein the dissolving temperature in step 3) is 40-50 ℃ and the dissolving time is 20-160 min.
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CN102964175A (en) * 2012-11-12 2013-03-13 北京依科曼生物技术有限公司 Composite amino-acid-containing water-soluble fertilizer and preparation method thereof
CN103172437A (en) * 2012-12-31 2013-06-26 天津北洋百川生物技术有限公司 Liquid compound microbial fertilizer containing polyglutamic acid, and preparation method and application thereof
CN104557155A (en) * 2013-10-10 2015-04-29 江西省新龙生物科技有限公司 Preparation method of insect body peptide plant physiology conditioning agent
CN103588585A (en) * 2013-11-28 2014-02-19 成都绿金生物科技有限责任公司 Preparation method of amino acid water-soluble fertilizer containing polyaspartic acid
CN103626581A (en) * 2013-11-28 2014-03-12 成都绿金生物科技有限责任公司 Method for preparing abscisic-acid-containing amino acid water-soluble fertilizer
CN105906403A (en) * 2016-07-08 2016-08-31 史丹利化肥股份有限公司 Long-acting fruit-expanding liquid fertilizer and preparation method thereof
CN109627100A (en) * 2019-01-31 2019-04-16 武汉楚强生物科技有限公司 One planting fruit-trees organic water-soluble fertilizer
CN110028354A (en) * 2019-04-22 2019-07-19 河南捷农生化有限公司 One kind of multiple chelating amino acid fertilizers and preparation method thereof

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* Cited by examiner, † Cited by third party
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CN115611680A (en) * 2022-09-09 2023-01-17 中国科学院遗传与发育生物学研究所农业资源研究中心 Special multifunctional compound agent for alfalfa in cold and dry areas and use method of special multifunctional compound agent
CN115611680B (en) * 2022-09-09 2024-05-17 中国科学院遗传与发育生物学研究所农业资源研究中心 Multifunctional compound agent special for alfalfa in cold and dry regions and application method of multifunctional compound agent

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Application publication date: 20200114